RchiOBHm_Chr2g0146311

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014812
Physical Location & Seq
Reverse (-)
63964787 .. 63965212
426 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51607

Sequence Viewer

Length: 318 bp
ATGAGTAATGTACTTGTGAGCTGTGTGTTATTTTGCATTCTTGCTTCAACAGAATTTAGAATTGGTTATGGCCAAACGACTTATAATGTACTCGACCATGGTGCAGTTGGAGATGGCCAAAGAGATGATTCTGAAGCTTTCTTGAAAGCATGGAACGCCGCATGTCTAGATAAAGGGACACCAAGACTTGTAATACCAGGGGAGAAGACATTTTTACTTCAACCTACCAAATTCTCAGGTCCTTGCCAGTCCAGCTCCATTCAGGTTGAGGTAAGAAACCAAGTAGCGGATGAAACTGGAGAAGTGTTTTCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

105

Amino Acids

11.38

Weight (kDa)

4.75

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 84
AciI CCGC 2 cut(s) 159, 287
AcoI YGGCCR 2 cut(s) 70, 115
AcsI RAATTY 2 cut(s) 53, 230
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 2 cut(s) 12, 90
AfiI CCNNNNNNNGG 1 cut(s) 286
AgsI TTSAA 3 cut(s) 48, 145, 221
AjnI CCWGG 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AluBI AGCT 3 cut(s) 21, 137, 255
AluI AGCT 3 cut(s) 21, 137, 255
AoxI GGCC 2 cut(s) 70, 115
ApoI RAATTY 2 cut(s) 53, 230
AspS9I GGNCC 1 cut(s) 239
AvaII GGWCC 1 cut(s) 239
BalI TGGCCA 2 cut(s) 72, 117
BbsI GAAGAC 1 cut(s) 212
BccI CCATC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 1 cut(s) 198
BfaI CTAG 1 cut(s) 167
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 97, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 2 cut(s) 247, 301
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 97, 197
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 2 cut(s) 247, 301
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 72, 117
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 2 cut(s) 72, 117
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 159, 287
BspANI GGCC 2 cut(s) 72, 117
BspCNI CTCAG 1 cut(s) 248
BsrI ACTGG 2 cut(s) 247, 301
BssECI CCNNGG 2 cut(s) 97, 197
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 155, 252
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 212
BsuRI GGCC 2 cut(s) 72, 117
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 11, 89
CviAII CATG 3 cut(s) 98, 150, 162
CviJI RGCY 5 cut(s) 21, 72, 117, 137, 255
CviKI_1 RGCY 5 cut(s) 21, 72, 117, 137, 255
CviQI GTAC 2 cut(s) 11, 89
DdeI CTNAG 1 cut(s) 235
EaeI YGGCCR 2 cut(s) 70, 115
Eco130I CCWWGG 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 101, 153, 165
FaiI YATR 5 cut(s) 69, 84, 99, 151, 163
FaqI GGGAC 1 cut(s) 190
FatI CATG 3 cut(s) 97, 149, 161
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 167
GluI GCNGC 1 cut(s) 159
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 2 cut(s) 72, 117
Hin1II CATG 3 cut(s) 101, 153, 165
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 2 cut(s) 142, 167
HpyCH4V TGCA 2 cut(s) 36, 104
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 252
HpyF3I CTNAG 1 cut(s) 235
Hsp92II CATG 3 cut(s) 101, 153, 165
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 7 cut(s) 183, 210, 222, 248, 260, 265, 282
MaeI CTAG 1 cut(s) 167
MboII GAAGA 1 cut(s) 217
MlsI TGGCCA 2 cut(s) 72, 117
MluCI AATT 3 cut(s) 53, 60, 230
MluNI TGGCCA 2 cut(s) 72, 117
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 1 cut(s) 262
Mox20I TGGCCA 2 cut(s) 72, 117
MscI TGGCCA 2 cut(s) 72, 117
Msp20I TGGCCA 2 cut(s) 72, 117
MspR9I CCNGG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 155, 252
NcoI CCATGG 1 cut(s) 97
NlaIII CATG 3 cut(s) 101, 153, 165
NspI RCATGY 1 cut(s) 165
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 239
PsiI TTATAA 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 239
PspPPI RGGWCCY 1 cut(s) 239
RsaI GTAC 2 cut(s) 12, 90
RsaNI GTAC 2 cut(s) 11, 89
SatI GCNGC 1 cut(s) 159
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 198
SetI ASST 7 cut(s) 23, 139, 226, 241, 257, 267, 273
SinI GGWCC 1 cut(s) 239
Sse9I AATT 3 cut(s) 53, 60, 230
SsiI CCGC 2 cut(s) 159, 287
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 97
TaqI TCGA 1 cut(s) 93
TasI AATT 3 cut(s) 53, 60, 230
TatI WGTACW 2 cut(s) 10, 88
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 128
TspDTI ATGAA 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 2 cut(s) 53, 230
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.