Rorug06G0113100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014812
Physical Location & Seq
Forward (+)
15417206 .. 15417961
756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0113100.1

Sequence Viewer

Length: 756 bp
ATGTTGTGGCAAGGACGTCCGCCGAAAACCTTGCCGGCAGTGGAGAAATTTTGGCAGGAGGGAAACTGGGCTTCTGCTAGGTTGACTTGTATTTGGGCTTGGGGGCCCAAGAGTTTGACATATTTGGGCAGTTTGGACCTTGAAAGCCCCAAAAAGCCTATATGTTTGACTCCTGACACCGTTCCCACAGCTGAATGGAATTCATTTCAGGAAGTTTGCGAGGAAAATGCAGACTTGCAATCATGGGACACAGACCTCAAAATCCATGCAGAAGAGAAGGGTGAAGGAGGTCTCTCGATTGATTCTTGGAAACCATTCACACTCTATGCATGGTATTCAAACAAGAAGATTGTGCAAGCAATCTTGGAATTCAAGGAAGATATAACTAAAGGGAAGGGGATTAGTAAGAAGAAGAAGACAAATGAAGAAAGAGTGAACATCCTGGCTGACGAGTATTATAAGAATAGCTCAAACATGTTGGATTTTCTTTCTGAAATGGAAAACTTCCTCATGCTTGCTCGTAATAGCCATTCATATATTGCAACAGTGATTCAAAAGTACTTTGAGATCCACAATGGCATGAGTGGAGCTGAGTTCTACGGGATGATTACGGATAAATTAAAAAAGCTCAAGGACTCTGATAATACCTTTGACCATTTTGCTAAAAACTTGCTCAAAAAGGCAGTGAGTTTGAAGAGCCAGCATGAAGAAATGATTCATAATTTGCAAGTCGAAATCAATAAACTTAGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

28.91

Weight (kDa)

6.11

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 95 - 249 3.4e-09 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AatII GACGTC 1 cut(s) 19
AciI CCGC 1 cut(s) 20
AclWI GGATC 1 cut(s) 562
AcsI RAATTY 3 cut(s) 47, 199, 368
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 2 cut(s) 560, 751
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 5 cut(s) 143, 339, 373, 554, 694
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 4 cut(s) 191, 468, 590, 628
AluI AGCT 4 cut(s) 191, 468, 590, 628
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 562
AoxI GGCC 1 cut(s) 104
ApaI GGGCCC 1 cut(s) 108
ApoI RAATTY 3 cut(s) 47, 199, 368
Asp700I GAANNNNTTC 2 cut(s) 314, 714
AspS9I GGNCC 3 cut(s) 104, 105, 136
AsuHPI GGTGA 1 cut(s) 293
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 422
BcgI CGANNNNNNTGC 4 cut(s) 13, 47, 209, 243
BciT130I CCWGG 1 cut(s) 443
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 78
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 104, 105, 136
BmiI GGNNCC 2 cut(s) 105, 106
BmrFI CCNGG 1 cut(s) 443
BmrI ACTGGG 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 614
BsaAI YACGTR 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 1 cut(s) 443
BseGI GGATG 2 cut(s) 438, 609
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 108
BshFI GGCC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 1 cut(s) 106
Bso31I GGTCTC 1 cut(s) 296
Bsp120I GGGCCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 567
BspACI CCGC 1 cut(s) 20
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 2 cut(s) 105, 106
BspPI GGATC 1 cut(s) 562
BspQI GCTCTTC 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 34
BssMI GATC 1 cut(s) 567
BssNI GRCGYC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 2 cut(s) 181, 547
Bst6I CTCTTC 2 cut(s) 267, 689
BstACI GRCGYC 1 cut(s) 16
BstBAI YACGTR 1 cut(s) 753
BstC8I GCNNGC 4 cut(s) 36, 357, 516, 701
BstDEI CTNAG 2 cut(s) 591, 746
BstF5I GGATG 2 cut(s) 438, 609
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 567
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 108
BstSNI TACGTA 1 cut(s) 753
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 2 cut(s) 438, 609
BtsI GCAGTG 2 cut(s) 45, 690
BtsIMutI CAGTG 3 cut(s) 45, 552, 690
Cac8I GCNNGC 4 cut(s) 36, 357, 516, 701
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 104, 105, 136
Csp6I GTAC 2 cut(s) 559, 750
CviAII CATG 7 cut(s) 243, 266, 330, 475, 511, 580, 704
CviQI GTAC 2 cut(s) 559, 750
DdeI CTNAG 2 cut(s) 591, 746
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
Eam1104I CTCTTC 2 cut(s) 267, 689
EarI CTCTTC 2 cut(s) 267, 689
EciI GGCGGA 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 753
Eco24I GRGCYC 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 2 cut(s) 199, 368
EcoRII CCWGG 1 cut(s) 441
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 7 cut(s) 246, 269, 333, 478, 514, 583, 707
FaqI GGGAC 1 cut(s) 260
FatI CATG 7 cut(s) 242, 265, 329, 474, 510, 579, 703
FokI GGATG 2 cut(s) 425, 616
FriOI GRGCYC 1 cut(s) 108
FspBI CTAG 1 cut(s) 78
HaeIII GGCC 1 cut(s) 106
HapII CCGG 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 7 cut(s) 246, 269, 333, 478, 514, 583, 707
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 5 cut(s) 169, 302, 550, 635, 715
HpaII CCGG 1 cut(s) 35
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 84, 436
Hpy188I TCNGA 2 cut(s) 493, 640
Hpy188III TCNNGA 3 cut(s) 173, 209, 295
Hpy8I GTNNAC 2 cut(s) 84, 436
HpyAV CCTTC 3 cut(s) 271, 278, 388
HpyCH4III ACNGT 2 cut(s) 181, 547
HpyCH4IV ACGT 2 cut(s) 16, 752
HpyCH4V TGCA 7 cut(s) 230, 238, 269, 329, 355, 542, 727
HpyF3I CTNAG 2 cut(s) 591, 746
HpySE526I ACGT 2 cut(s) 16, 752
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 7 cut(s) 246, 269, 333, 478, 514, 583, 707
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 1 cut(s) 567
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 1 cut(s) 587
LpnPI CCDG 8 cut(s) 41, 48, 52, 186, 194, 428, 455, 713
MaeI CTAG 1 cut(s) 78
MaeII ACGT 2 cut(s) 16, 752
MalI GATC 1 cut(s) 569
MboI GATC 1 cut(s) 567
MboII GAAGA 9 cut(s) 284, 358, 389, 421, 424, 427, 437, 706, 719
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 5 cut(s) 47, 199, 368, 617, 721
MlyI GAGTC 2 cut(s) 163, 629
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 52, 214, 266, 281, 518
Mph1103I ATGCAT 1 cut(s) 331
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 2 cut(s) 314, 714
MseI TTAA 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 443
MvaI CCWGG 1 cut(s) 443
NaeI GCCGGC 1 cut(s) 36
NdeII GATC 1 cut(s) 567
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 7 cut(s) 246, 269, 333, 478, 514, 583, 707
NlaIV GGNNCC 2 cut(s) 105, 106
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 478
PciI ACATGT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 689
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 314, 714
PfeI GAWTC 3 cut(s) 302, 550, 715
PleI GAGTC 2 cut(s) 163, 629
PpsI GAGTC 2 cut(s) 163, 629
Ppu21I YACGTR 1 cut(s) 753
PscI ACATGT 1 cut(s) 474
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 105, 106
PspOMI GGGCCC 1 cut(s) 104
PspPI GGNCC 3 cut(s) 104, 105, 136
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 560, 751
RsaNI GTAC 2 cut(s) 559, 750
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 1 cut(s) 620
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 3 cut(s) 104, 105, 136
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 2 cut(s) 163, 629
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 1 cut(s) 108
SinI GGWCC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SnaBI TACGTA 1 cut(s) 753
Sse9I AATT 5 cut(s) 47, 199, 368, 617, 721
SsiI CCGC 1 cut(s) 20
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 181, 547
TaiI ACGT 2 cut(s) 19, 755
TaqI TCGA 2 cut(s) 296, 732
TasI AATT 5 cut(s) 47, 199, 368, 617, 721
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 3 cut(s) 302, 550, 715
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 3 cut(s) 45, 552, 690
TspDTI ATGAA 5 cut(s) 192, 438, 522, 707, 720
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 3 cut(s) 45, 552, 690
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 3 cut(s) 47, 199, 368
XceI RCATGY 1 cut(s) 478
XmnI GAANNNNTTC 2 cut(s) 314, 714
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.