Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003174

Physical Location & Seq

Genomic Coordinates Reverse (-)

1154156 .. 1159393

Gene Structure 5238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1010600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGGAACGAGACCGAGCCCAGCCTGCAGAGTCGACGAAGAGCGATCTCGAAAACGTGAAGGTGATCGAATGGGAGGAATTCGAGCAAGAGATTGTGAGGTTATGGAGTCTTTCTTCTGCTTTTAGCGAAGCTCAGGAGAAGAAGCAGAGTCTTCAGCGGAAGCTCGAGTTTCTTATTGAGGTTGAAGCTGAGTCATTGAGACGATCAAATGAGCTTGAGGAGATGCAGCAGAAACTGGAAGCGAGAAAAATGGTGATGGGAAACATGTCAAGGCATTCTATGCTTGCTGTGGAGAATACAAAAAAGAAAGAGGAGCAGCTTAGTATAGAGCTTCGGTCGCTATTGGTTGCTGGGACGTCTCTATCTGTTGCTAGGAAGCGGTTGGAGGAGTCCAATCAGCTACTTTCCAGAGACAAGGGCTATGTTCGTCTTAAAAGCTTACAAAAGATGCTAAGAAGGCGACAACAACATATGATATCACAAGTTTCGTTGCTATATCCTGTAAAGTTCACTATGGGTCCTGCACAAGAACAGGAACTTGAGTCATTTCCAAACACTAGTAGATCAGGTAATTCTGCTCGATTTAAGCCTGCAAATCAAGGAACATTGACAATTTTAGGTCTCCATTTGACAATGCTGCCTTTTAAAATGATGAGTTTTTTCACTGACAAAAAGGAGGTTCAGAAGTCTGCAACAGCCCTGGGATATGTCGGTCATGCTGTCTTACTTATAGCTTCCTACTTAAAAGTTCCGTTGCGTTATCCATTGCGTTTGGGTGGTTCCCATTCCTATATTATTGATTATGGACCATCCGTAGAGCCTACTTCATCCGATGTGTCGTCAAATACAGTGGTTTCAACGGATGTGAAGCATGTGGAGTTCCCTCTCTTTTTAGATGGCCAAGACACAACGAGAGCCGCATATGCTGTATTCTTATTAAACAAGGATTTAGAGCAGCTTTTAAATTACATTGGCGTTAAGAGCTTAGGGCCACGCAATGTCCTTGCTAATTTGAAGGAGCTTGTAAGGACTATTCAATCTGTAGATTATATGGATACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04140 map04140

Pfam Domains

Protein Families

Atg14

Protein Analysis

354

Amino Acids

40.2

Weight (kDa)

8.97

Isoelectric Point (pI)

55.89

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003174)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32760 AT2G32760 AT2G32760
fragaria_vesca	FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790 FvH4_4g13790
malus_domestica	MD00G1113200.v1.1 MD04G1010600.v1.1
prunus_persica	Prupe.1G010300_v2.0.a1 Prupe.1G010300_v2.0.a1 Prupe.1G010300_v2.0.a1
pyrus_communis	pycom04g00870 pycom420g01290
rosa_chinensis	RchiOBHm_Chr4g0414711
rosa_laevigata	RLG00000008122
rosa_multiflora	Rmu_sc0001192.1_g000027 Rmu_sc0010606.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358520
rosa_rugosa	Rorug04G0129800 Rorug04G0129900 Rorug04G0130000 Rorug04G0130100 Rorug04G0130200 Rorug04G0130200 Rorug04G0130200
rosa_samantha	Rh4AG188300 Rh4BG187300 Rh4CG200600 Rh4DG187000
rosa_wichuraiana	Rw4G016070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	359
AccI	GTMKAC	1 cut(s)	32
AciI	CCGC	3 cut(s)	157, 379, 918
AcoI	YGGCCR	1 cut(s)	898
AcsI	RAATTY	1 cut(s)	77
AcuI	CTGAAG	1 cut(s)	137
AcyI	GRCGYC	1 cut(s)	356
AflIII	ACRYGT	1 cut(s)	264
AgsI	TTSAA	4 cut(s)	185, 858, 1015, 1037
AhlI	ACTAGT	1 cut(s)	557
AjnI	CCWGG	1 cut(s)	699
Alw26I	GTCTC	5 cut(s)	3, 193, 363, 405, 626
AlwNI	CAGNNNCTG	1 cut(s)	235
Ama87I	CYCGRG	1 cut(s)	164
AoxI	GGCC	2 cut(s)	898, 989
ApeKI	GCWGC	4 cut(s)	226, 316, 637, 955
ApoI	RAATTY	1 cut(s)	77
AspS9I	GGNCC	3 cut(s)	518, 806, 989
AsuHPI	GGTGA	2 cut(s)	73, 265
AvaI	CYCGRG	1 cut(s)	164
AvaII	GGWCC	2 cut(s)	518, 806
BalI	TGGCCA	1 cut(s)	900
BanII	GRGCYC	1 cut(s)	19
BbsI	GAAGAC	1 cut(s)	143
BbvI	GCAGC	4 cut(s)	238, 328, 624, 967
BccI	CCATC	3 cut(s)	250, 817, 890
BciT130I	CCWGG	1 cut(s)	701
BciVI	GTATCC	1 cut(s)	1048
BcoDI	GTCTC	5 cut(s)	3, 193, 363, 405, 626
BcuI	ACTAGT	1 cut(s)	557
BfaI	CTAG	2 cut(s)	372, 558
BfmI	CTRYAG	2 cut(s)	24, 1041
BfuI	GTATCC	1 cut(s)	1048
BisI	GCNGC	5 cut(s)	227, 317, 638, 918, 956
BlsI	GCNGC	5 cut(s)	228, 318, 639, 919, 957
Bme1390I	CCNGG	1 cut(s)	701
Bme18I	GGWCC	2 cut(s)	518, 806
BmeT110I	CYCGRG	1 cut(s)	164
BmgT120I	GGNCC	3 cut(s)	518, 806, 989
BmiI	GGNNCC	2 cut(s)	519, 781
BmrFI	CCNGG	1 cut(s)	701
BmsI	GCATC	2 cut(s)	213, 438
BpiI	GAAGAC	1 cut(s)	143
Bpu10I	CCTNAGC	2 cut(s)	132, 985
BpuEI	CTTGAG	2 cut(s)	236, 560
BsaAI	YACGTR	1 cut(s)	1059
BsaHI	GRCGYC	1 cut(s)	356
BsaI	GGTCTC	2 cut(s)	3, 626
BsaJI	CCNNGG	2 cut(s)	699, 700
Bse1I	ACTGG	1 cut(s)	240
Bse3DI	GCAATG	2 cut(s)	764, 1003
BseBI	CCWGG	1 cut(s)	701
BseDI	CCNNGG	2 cut(s)	699, 700
BseGI	GGATG	3 cut(s)	809, 827, 868
BseMI	GCAATG	2 cut(s)	764, 1003
BseMII	CTCAG	2 cut(s)	146, 180
BseNI	ACTGG	1 cut(s)	240
BseRI	GAGGAG	3 cut(s)	233, 326, 401
BseXI	GCAGC	4 cut(s)	238, 328, 624, 967
BseYI	CCCAGC	2 cut(s)	18, 350
BsgI	GTGCAG	1 cut(s)	507
Bsh1285I	CGRYCG	1 cut(s)	338
BshFI	GGCC	2 cut(s)	900, 991
BsiEI	CGRYCG	1 cut(s)	338
BsiHKCI	CYCGRG	1 cut(s)	164
BslFI	GGGAC	1 cut(s)	367
BsmAI	GTCTC	5 cut(s)	3, 193, 363, 405, 626
BsmBI	CGTCTC	2 cut(s)	193, 363
BsmFI	GGGAC	1 cut(s)	367
BsmI	GAATGC	1 cut(s)	274
BsnI	GGCC	2 cut(s)	900, 991
Bso31I	GGTCTC	2 cut(s)	3, 626
BsoBI	CYCGRG	1 cut(s)	164
Bsp1286I	GDGCHC	1 cut(s)	19
Bsp143I	GATC	4 cut(s)	43, 63, 203, 563
BspACI	CCGC	3 cut(s)	157, 379, 918
BspANI	GGCC	2 cut(s)	900, 991
BspCNI	CTCAG	2 cut(s)	145, 181
BspLI	GGNNCC	2 cut(s)	519, 781
BspMAI	CTGCAG	1 cut(s)	28
BspQI	GCTCTTC	1 cut(s)	32
BspTNI	GGTCTC	2 cut(s)	3, 626
BsrDI	GCAATG	2 cut(s)	764, 1003
BsrI	ACTGG	1 cut(s)	240
BssECI	CCNNGG	2 cut(s)	699, 700
BssMI	GATC	4 cut(s)	43, 63, 203, 563
BssNI	GRCGYC	1 cut(s)	356
Bst2UI	CCWGG	1 cut(s)	701
Bst4CI	ACNGT	1 cut(s)	850
Bst6I	CTCTTC	1 cut(s)	32
BstACI	GRCGYC	1 cut(s)	356
BstAPI	GCANNNNNTGC	1 cut(s)	280
BstBAI	YACGTR	1 cut(s)	1059
BstC8I	GCNNGC	3 cut(s)	24, 285, 591
BstDEI	CTNAG	5 cut(s)	132, 189, 320, 452, 985
BstF5I	GGATG	3 cut(s)	809, 827, 868
BstKTI	GATC	4 cut(s)	46, 66, 206, 566
BstMAI	GTCTC	5 cut(s)	3, 193, 363, 405, 626
BstMBI	GATC	4 cut(s)	43, 63, 203, 563
BstMCI	CGRYCG	1 cut(s)	338
BstMWI	GCNNNNNNNGC	6 cut(s)	23, 280, 337, 457, 923, 981
BstNI	CCWGG	1 cut(s)	701
BstNSI	RCATGY	2 cut(s)	268, 875
BstSCI	CCNGG	1 cut(s)	699
BstSFI	CTRYAG	2 cut(s)	24, 1041
BstV1I	GCAGC	4 cut(s)	238, 328, 624, 967
BstV2I	GAAGAC	1 cut(s)	143
BsuI	GTATCC	1 cut(s)	1048
BsuRI	GGCC	2 cut(s)	900, 991
BtsCI	GGATG	3 cut(s)	809, 827, 868
BtsIMutI	CAGTG	2 cut(s)	663, 855
Cac8I	GCNNGC	3 cut(s)	24, 285, 591
CaiI	CAGNNNCTG	1 cut(s)	235
Cfr13I	GGNCC	3 cut(s)	518, 806, 989
CspCI	CAANNNNNGTGG	2 cut(s)	831, 866
CviAII	CATG	3 cut(s)	265, 716, 872
DdeI	CTNAG	5 cut(s)	132, 189, 320, 452, 985
DpnI	GATC	4 cut(s)	45, 65, 205, 565
DpnII	GATC	4 cut(s)	43, 63, 203, 563
DraI	TTTAAA	2 cut(s)	646, 963
EaeI	YGGCCR	1 cut(s)	898
Eam1104I	CTCTTC	1 cut(s)	32
EarI	CTCTTC	1 cut(s)	32
Eco24I	GRGCYC	1 cut(s)	19
Eco31I	GGTCTC	2 cut(s)	3, 626
Eco32I	GATATC	1 cut(s)	477
Eco47I	GGWCC	2 cut(s)	518, 806
Eco57I	CTGAAG	1 cut(s)	137
Eco88I	CYCGRG	1 cut(s)	164
EcoO109I	RGGNCCY	1 cut(s)	518
EcoRI	GAATTC	1 cut(s)	77
EcoRII	CCWGG	1 cut(s)	699
EcoRV	GATATC	1 cut(s)	477
EcoT38I	GRGCYC	1 cut(s)	19
Esp3I	CGTCTC	2 cut(s)	193, 363
FaeI	CATG	3 cut(s)	268, 719, 875
FaqI	GGGAC	1 cut(s)	367
FatI	CATG	3 cut(s)	264, 715, 871
FauNDI	CATATG	2 cut(s)	471, 922
FblI	GTMKAC	1 cut(s)	32
Fnu4HI	GCNGC	5 cut(s)	227, 317, 638, 918, 956
FokI	GGATG	3 cut(s)	796, 814, 875
FriOI	GRGCYC	1 cut(s)	19
Fsp4HI	GCNGC	5 cut(s)	227, 317, 638, 918, 956
FspBI	CTAG	2 cut(s)	372, 558
GluI	GCNGC	5 cut(s)	227, 317, 638, 918, 956
GsaI	CCCAGC	2 cut(s)	22, 354
HaeIII	GGCC	2 cut(s)	900, 991
Hin1I	GRCGYC	1 cut(s)	356
Hin1II	CATG	3 cut(s)	268, 719, 875
HincII	GTYRAC	1 cut(s)	33
HindII	GTYRAC	1 cut(s)	33
HindIII	AAGCTT	1 cut(s)	436
HinfI	GANTC	6 cut(s)	29, 106, 148, 191, 389, 542
HphI	GGTGA	2 cut(s)	73, 265
Hpy166II	GTNNAC	2 cut(s)	33, 510
Hpy188I	TCNGA	2 cut(s)	684, 832
Hpy188III	TCNNGA	3 cut(s)	47, 134, 408
Hpy8I	GTNNAC	2 cut(s)	33, 510
Hpy99I	CGWCG	1 cut(s)	37
HpyAV	CCTTC	3 cut(s)	52, 450, 1009
HpyCH4III	ACNGT	1 cut(s)	850
HpyCH4IV	ACGT	3 cut(s)	54, 356, 1058
HpyCH4V	TGCA	5 cut(s)	26, 226, 524, 593, 692
HpyF10VI	GCNNNNNNNGC	6 cut(s)	23, 280, 337, 457, 923, 981
HpyF3I	CTNAG	5 cut(s)	132, 189, 320, 452, 985
HpySE526I	ACGT	3 cut(s)	54, 356, 1058
Hsp92I	GRCGYC	1 cut(s)	356
Hsp92II	CATG	3 cut(s)	268, 719, 875
Kzo9I	GATC	4 cut(s)	43, 63, 203, 563
LguI	GCTCTTC	1 cut(s)	32
LmnI	GCTCC	2 cut(s)	313, 1018
Lsp1109I	GCAGC	4 cut(s)	238, 328, 624, 967
LweI	GCATC	2 cut(s)	213, 438
MaeI	CTAG	2 cut(s)	372, 558
MaeII	ACGT	3 cut(s)	54, 356, 1058
MalI	GATC	4 cut(s)	45, 65, 205, 565
MboI	GATC	4 cut(s)	43, 63, 203, 563
MboII	GAAGA	4 cut(s)	49, 105, 143, 151
MhlI	GDGCHC	1 cut(s)	19
MlsI	TGGCCA	1 cut(s)	900
MluCI	AATT	5 cut(s)	77, 571, 612, 964, 1009
MluNI	TGGCCA	1 cut(s)	900
MlyI	GAGTC	6 cut(s)	38, 115, 157, 200, 398, 551
MmeI	TCCRAC	1 cut(s)	363
MnlI	CCTC	8 cut(s)	67, 90, 172, 211, 304, 379, 670, 894
Mox20I	TGGCCA	1 cut(s)	900
MscI	TGGCCA	1 cut(s)	900
MseI	TTAA	7 cut(s)	432, 585, 645, 743, 938, 962, 978
Msp20I	TGGCCA	1 cut(s)	900
MspA1I	CMGCKG	1 cut(s)	157
MspR9I	CCNGG	1 cut(s)	701
Mva1269I	GAATGC	1 cut(s)	274
MvaI	CCWGG	1 cut(s)	701
MwoI	GCNNNNNNNGC	6 cut(s)	23, 280, 337, 457, 923, 981
NdeI	CATATG	2 cut(s)	471, 922
NdeII	GATC	4 cut(s)	43, 63, 203, 563
NlaIII	CATG	3 cut(s)	268, 719, 875
NlaIV	GGNNCC	2 cut(s)	519, 781
NspI	RCATGY	2 cut(s)	268, 875
PaeR7I	CTCGAG	1 cut(s)	164
PasI	CCCWGGG	1 cut(s)	700
PciI	ACATGT	1 cut(s)	264
PciSI	GCTCTTC	1 cut(s)	32
PctI	GAATGC	1 cut(s)	274
PkrI	GCNGC	5 cut(s)	228, 318, 639, 919, 957
PleI	GAGTC	6 cut(s)	37, 114, 156, 199, 397, 550
PpsI	GAGTC	6 cut(s)	37, 114, 156, 199, 397, 550
Ppu21I	YACGTR	1 cut(s)	1059
PpuMI	RGGWCCY	1 cut(s)	518
PscI	ACATGT	1 cut(s)	264
Psp5II	RGGWCCY	1 cut(s)	518
Psp6I	CCWGG	1 cut(s)	699
PspFI	CCCAGC	2 cut(s)	18, 350
PspGI	CCWGG	1 cut(s)	699
PspN4I	GGNNCC	2 cut(s)	519, 781
PspPI	GGNCC	3 cut(s)	518, 806, 989
PspPPI	RGGWCCY	1 cut(s)	518
PspXI	VCTCGAGB	1 cut(s)	164
PstI	CTGCAG	1 cut(s)	28
PstNI	CAGNNNCTG	1 cut(s)	235
SalI	GTCGAC	1 cut(s)	31
SapI	GCTCTTC	1 cut(s)	32
SaqAI	TTAA	7 cut(s)	432, 585, 645, 743, 938, 962, 978
SatI	GCNGC	5 cut(s)	227, 317, 638, 918, 956
Sau3AI	GATC	4 cut(s)	43, 63, 203, 563
Sau96I	GGNCC	3 cut(s)	518, 806, 989
SchI	GAGTC	6 cut(s)	38, 115, 157, 200, 398, 551
ScrFI	CCNGG	1 cut(s)	701
SduI	GDGCHC	1 cut(s)	19
SfaNI	GCATC	2 cut(s)	213, 438
SfcI	CTRYAG	2 cut(s)	24, 1041
Sfr274I	CTCGAG	1 cut(s)	164
SinI	GGWCC	2 cut(s)	518, 806
SlaI	CTCGAG	1 cut(s)	164
SmlI	CTYRAG	3 cut(s)	164, 215, 539
SmoI	CTYRAG	3 cut(s)	164, 215, 539
SpeI	ACTAGT	1 cut(s)	557
Sse9I	AATT	5 cut(s)	77, 571, 612, 964, 1009
SsiI	CCGC	3 cut(s)	157, 379, 918
SspMI	CTAG	2 cut(s)	372, 558
StyD4I	CCNGG	1 cut(s)	699
TaaI	ACNGT	1 cut(s)	850
TaiI	ACGT	3 cut(s)	57, 359, 1061
TaqI	TCGA	6 cut(s)	32, 48, 66, 81, 165, 580
TaqII	GACCGA	3 cut(s)	27, 324, 701
TasI	AATT	5 cut(s)	77, 571, 612, 964, 1009
TauI	GCSGC	1 cut(s)	920
Tru1I	TTAA	7 cut(s)	432, 585, 645, 743, 938, 962, 978
Tru9I	TTAA	7 cut(s)	432, 585, 645, 743, 938, 962, 978
TscAI	CASTG	2 cut(s)	670, 855
TseI	GCWGC	4 cut(s)	226, 316, 637, 955
TspDTI	ATGAA	1 cut(s)	816
TspGWI	ACGGA	3 cut(s)	741, 802, 875
TspRI	CASTG	2 cut(s)	670, 855
VpaK11BI	GGWCC	2 cut(s)	518, 806
XapI	RAATTY	1 cut(s)	77
XceI	RCATGY	2 cut(s)	268, 875
XhoI	CTCGAG	1 cut(s)	164
XmiI	GTMKAC	1 cut(s)	32
XspI	CTAG	2 cut(s)	372, 558
ZraI	GACGTC	1 cut(s)	357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.