Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008467

Physical Location & Seq

Genomic Coordinates Reverse (-)

3014135 .. 3029365

Gene Structure 15231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1025900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3552 bp

ATGGATTGCGTGCATGCGCTGCACACCTCTTCTTCCGCGCCACAACTCCACCGTCGCAAGCAACTCCGATGCCTCCACCCTCGGCATCAATGCCTGGGAAGCCTTGCCGTCGTTCCCTTCTTCGCCCATAAGAGGGGTTTTCGTCCCCTCTACCACAAATCCAACACTTGTCAGATCATTTGTGGCGTTTCCTCCGCTCAATCAATTGACGAGGAGAAAGACAGCATGATGAAGTCCGAGTCTGGGAAAGTTCGGCTTAATATTCGATTGGATCACCAAGTTGAATTTGGTGAGAGTGTTGCAATATTGGGTTCGGTGAAGGAATTGGGATCATGGAAGAAGAAGGTGCCTATGAATTGGACAGAGAGCGGATGGGTATGTACACTGGAGTTCAAAGGGGGTGAGTCTGTTGAGTATAAGTTTTTGACTGTAAGGGCAGACAAGAGTATGCTTTGGGAAGGTGGTCACAATCGGGTCTTGAAACTTCCCAATGGAGGAAGTTTTGAGATGGTTTGTCATTGGAATGCAACTACGGAAGCTGTGGGTTTACCCTCTTCGGAGGAAGGGGAGGATGTGGGTCAGAACGGTTCTACAGTGGCTGATACTGTTGGTGCTGAAGAGGTGGAGTCTAGTCCTTTTGTTGGTCAATGGAAAGGTAATGCCATCTCATTCATGCGGTCGAATGAGCATGGGAACCGTAAAGGAGGAAAATGGGACACCTCTGGTCTTGAAGGGTTGGCTCTGAAGTTAGTTGAAGGTGATCGAAATGCAAGGAATTGGTGGAAGAAGCTTGAAGTTGTACGTGATCTACTTGTTGGAAGTTTGCAAAGTGAGGACCGGTTGGATGCTCTCATAAATTCTGCCATTTATTTAAAGTGGATAAATACAGGCCAGATTCCTTGCTTTGAAGATGGAGGTCATCATCGACCAAACCGACATGCTGAGATTTCCAGGGTTATATTTCGAGAACTGGAAAGAATTTCCTGTAGGAAAGATACTTCACCCCAGGAAGTACTTGTCATCCGCAAAATTCATCCATGTCTGCCATCTTTCAAAGCAGAGTTTACTGCATCTGTTCCCCTTACACGAATAAGAGACATTGCTCATCGGAATGATATCCCTCATGACCTCAAGCAAGAAATTAAGCATACAATTCAAAACAAGCTTCACCGTAATGCCGGCCCTGAAGATCTAGTTGCTACAGAAGCTATGCTTGCAAGAATAACCAAGAACCCTGGAGAATACAATGGAGCATTTGTAGAGCAGTTCAAGATATTCCATCATGAACTCAAGGACTTCTTCAATGCTGGAAGCCTTGCCGAACAACTTGAATCCCTCAAAGACTCTATTGATGATAAAGGCCGTTCAGCTCTTGCATTGTTTCTGGAGTGCAAAAAGAGTTTGGATACTTTAGAAGTGTCAAATAAAGGTTTGGGAAATAATGGCACTGATCTGTTGTTCAAGACCATGCAATCTTTGAGTGCCCTGAGAGAAATAATTGCAAGGGGTCTTGAAAGTGGCCTTAGAAATGATGCTCCTGATAATGCAGTGGCTATGCGTCAGAAGTGGCGCCTTTGTGAGATTGGACTTGAGGACTATTCGTTTATTCTTTTAAGCAGATTCCTTAATGAGCTTGATGCTTTGGGAGGGGCACACTGGCTGGCAGAAAATGTGAAATCAAAGGATGTAAGCTCTTGGAATGGTCCACTTGATGCCCTTATTGTCGGGATTCATCAGCTAAGTTTATCTGGTTGGAAGCCTGAAGAATGTGCTGCTATTGAGAATGAACTTGTTGCATGGAAAGCAAGAGGTCTTTCTGAAAAGGAAGGAAGTGAAGATGGCAAAACAGTCTGGGCACTAAGGCTTAAAGCTACTCTTGATAGAGCCAGGAGGCTAACTGAAGAATATTCTGAAGCACTTCTTCAAATATTCCCCCAAAATGTCCAGATTTTAGGAAAAGCTTTTGGGATCCCTGAGAATAGTGTAAGGACATATGCTGAAGCTGAGATTCGTGCTGGTGTAATATTTCAGGTCTCAAAACTGTGCACCCTACTTTTAAAAGCTGCTAGAAGTACCATTGGTTCTCAAGGTTGGGATGTTATTGTGCCAGGAGCTGCTGTGGGAACATTAGTTGAGAGGATTGTCCCTGGGTCAATACCATCAACCTTGGAAGGACCAATTGTTCTTGTGGTTAACAGAGCAGATGGAGATGAAGAGGTTACAGCTGCTGGGAGTAACATTGTGGGAGTTGTACTTCTGCAAGAGCTTCCTCACTTATCTCATCTCGGTGTCAGAGCTCGACAAGAGAAGGTTGTGTTCGTGACATGTGAAGATGATGATAAAGTTGCCGATATACAAAAACACAAGGGGAAATGTGTGAGGTTAGAAGCATCCTCATCAGGTGTTGATATATATCCTTCATCAGAAAATAGCAATGGCCATTTGTCAGTGGAGAATCTTTCAGGTGATGGTGCACCCAGGGTTGAAGCACGTGGAAGTGATGGTCCTTCATGGTCTGCTACTAAAGCCAACTCCAATCAGGGGGTTTCGGCTGGAGGAGTTTTACTACTTGCTGATGCAGATGCGGAGACATCAGGTGCAAAGGCTGCAGCTTGTGGTCGTTTAGCTTCCTTGGCTGCAGAATCTGAGAAAGTTTATAATGACGACGGGGTACCAGCTTCATTCAAAGTCCCTTTGGGCGCAGTGATACCATTTGGCTCTATGGAGTTGGCATTGGAACAAAGCAAATCCACCGAATTATTTAGGTCGTTACTAGATAAGGTAGAAACACAGAAGCTGGAAGGTGGGGAACTTGAACAACTTTGCAGTCAGCTCCAGGAACTAATCTCTTCCCTACAGCCCCCGAAAGACATTATTGATAGCATAGGGAAAATATTTCCTGAAAATGCATGCCTAATTGTTCGTTCAAGTGCTAATGTTGAGGACTTGGCTGGAATGTCAGCTGCTGGATTATATGACTCAATTCCCAATGTTAGTGTTTCAAATCCAACAGTCTTTGCAAATGCTATTAGCAGAGTATGGGCTTCACTTTACACTCGGAGGGCAGTATTGAGCCGTCGAGCTGCTGGTGTATCTCAGAAGGAAGCTACAATGGCAATTCTGGTGCAAGAAATGCTTTCACCAGACTTATCATTTGTGCTTCATACAGTCAGCCCAACGGACAAAAATCATAATTCTGTTGAGGCTGAAATTGCTTCTGGCCTTGGTGAAACTTTGGCTTCAGGCACTAGGGGCACACCCTGGCGTTTATCGTCTGGGAAATTTGATGGGAATGTACGAACTCTGGCATTTGCGAATTTCAGTGAGGAGCTAGTAGGTGCAGTCCTTGCTGATGGGGAGGTTATCCATTTGACTGTAGACTACAGCAAGAAACCGTTAACTGTTGATCCAATTTTCCGTCATCAGCTTGGTCAGCGCCTCAGTACAGTGGGATTCTTTCTTGAGCGGAAGTTTGGCTCCCCGCAGGATGTAGAAGGATGTCTTGTGGGCAAAGATATCTATATAGTCCAGACACGACCGCAACCCCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0019200 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046835 GO:0051752 GO:0071704 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 PPDK_N

Protein Analysis

1184

Amino Acids

129.21

Weight (kDa)

5.97

Isoelectric Point (pI)

37.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	85 - 167	7.2e-16	Starch binding domain
GWD1_pHisD	PF22973	698 - 810	3.8e-31	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	890 - 1181	2.2e-33	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26570 AT5G26570
fragaria_vesca	FvH4_4g12510 FvH4_4g12510
malus_domestica	MD04G1025900.v1.1
prunus_persica	Prupe.1G175200_v2.0.a1
pyrus_communis	pycom04g02200
rosa_chinensis	RchiOBHm_Chr4g0411251 RchiOBHm_Chr4g0411331
rosa_laevigata	RLG00000008365 RLG00000008368
rosa_multiflora	Rmu_sc0000543.1_g000027 Rmu_sc0000543.1_g000049
rosa_roxburghii	Rroxscaffold_5G00355910
rosa_rugosa	Rorug04G0110100
rosa_samantha	Rh4AG167100 Rh4BG163600 Rh4BG164200 Rh4CG178800 Rh4DG161900
rosa_wichuraiana	Rw4G013760 Rw4G013810 Rw4G013910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2658
Acc65I	GGTACC	1 cut(s)	2671
AccB1I	GGYRCC	3 cut(s)	346, 1569, 2671
AccBSI	CCGCTC	3 cut(s)	197, 369, 3466
AccI	GTMKAC	1 cut(s)	3378
AccII	CGCG	1 cut(s)	38
AciI	CCGC	9 cut(s)	36, 195, 369, 676, 1024, 2585, 3466, 3482, 3539
AclWI	GGATC	5 cut(s)	279, 337, 1963, 1976, 3401
AcoI	YGGCCR	1 cut(s)	2437
AcsI	RAATTY	6 cut(s)	284, 856, 978, 1029, 3281, 3316
AcuI	CTGAAG	8 cut(s)	636, 764, 1206, 1782, 1920, 1932, 2019, 3225
AcvI	CACGTG	1 cut(s)	2492
AcyI	GRCGYC	1 cut(s)	1570
AfaI	GTAC	8 cut(s)	382, 801, 1014, 2074, 2253, 2673, 3297, 3445
AfiI	CCNNNNNNNGG	6 cut(s)	132, 133, 243, 494, 641, 2541
AflIII	ACRYGT	1 cut(s)	2324
AgeI	ACCGGT	1 cut(s)	837
AloI	GAACNNNNNNTCC	2 cut(s)	2166, 2198
Alw21I	GWGCWC	3 cut(s)	2048, 2299, 2476
Alw26I	GTCTC	3 cut(s)	1089, 2038, 2582
Alw44I	GTGCAC	2 cut(s)	2044, 2472
AlwI	GGATC	5 cut(s)	279, 337, 1963, 1976, 3401
AlwNI	CAGNNNCTG	6 cut(s)	599, 2114, 2228, 2645, 2797, 2966
AoxI	GGCC	6 cut(s)	889, 1180, 1360, 1519, 2437, 3220
ApaLI	GTGCAC	2 cut(s)	2044, 2472
ApoI	RAATTY	6 cut(s)	284, 856, 978, 1029, 3281, 3316
ArsI	GACNNNNNNTTYG	2 cut(s)	3137, 3169
AsiGI	ACCGGT	1 cut(s)	837
Asp700I	GAANNNNTTC	1 cut(s)	1917
Asp718I	GGTACC	1 cut(s)	2671
AspLEI	GCGC	5 cut(s)	19, 40, 1572, 2702, 3438
AspS9I	GGNCC	5 cut(s)	835, 1181, 1703, 2174, 2504
AvaII	GGWCC	4 cut(s)	835, 1703, 2174, 2504
BaeGI	GKGCMC	6 cut(s)	1486, 1654, 1858, 2048, 2476, 3257
BalI	TGGCCA	1 cut(s)	2439
BamHI	GGATCC	1 cut(s)	1968
BanI	GGYRCC	3 cut(s)	346, 1569, 2671
BanII	GRGCYC	1 cut(s)	2299
BbrPI	CACGTG	1 cut(s)	2492
Bbv12I	GWGCWC	3 cut(s)	2048, 2299, 2476
BceAI	ACGGC	3 cut(s)	92, 1347, 3060
BciVI	GTATCC	1 cut(s)	1399
BcoDI	GTCTC	3 cut(s)	1089, 2038, 2582
BfaI	CTAG	6 cut(s)	630, 1193, 2067, 2774, 3249, 3332
BfmI	CTRYAG	8 cut(s)	591, 985, 1200, 2607, 2637, 2855, 3375, 3382
BfoI	RGCGCY	2 cut(s)	1573, 3439
BfuI	GTATCC	1 cut(s)	1399
BglII	AGATCT	1 cut(s)	1189
BmcAI	AGTACT	1 cut(s)	1014
Bme18I	GGWCC	4 cut(s)	835, 1703, 2174, 2504
BmgT120I	GGNCC	5 cut(s)	835, 1181, 1703, 2174, 2504
BmiI	GGNNCC	6 cut(s)	348, 695, 1571, 1970, 2673, 3478
BpmI	CTGGAG	5 cut(s)	407, 1257, 1406, 2574, 2819
BpuEI	CTTGAG	5 cut(s)	1115, 1274, 1610, 2070, 3482
BsaAI	YACGTR	2 cut(s)	803, 2492
BsaBI	GATNNNNATC	1 cut(s)	2412
BsaHI	GRCGYC	1 cut(s)	1570
BsaI	GGTCTC	1 cut(s)	2038
BsaWI	WCCGGW	1 cut(s)	837
BsaXI	ACNNNNNCTCC	4 cut(s)	1242, 1272, 2103, 2133
Bsc4I	CCNNNNNNNGG	6 cut(s)	132, 133, 243, 494, 641, 2541
Bse118I	RCCGGY	2 cut(s)	837, 1178
Bse1I	ACTGG	3 cut(s)	390, 975, 1661
Bse3DI	GCAATG	2 cut(s)	1098, 2440
Bse8I	GATNNNNATC	1 cut(s)	2412
BseJI	GATNNNNATC	1 cut(s)	2412
BseLI	CCNNNNNNNGG	6 cut(s)	132, 133, 243, 494, 641, 2541
BseMI	GCAATG	2 cut(s)	1098, 2440
BseMII	CTCAG	7 cut(s)	933, 1478, 1965, 1995, 2637, 3110, 3454
BseNI	ACTGG	3 cut(s)	390, 975, 1661
BseRI	GAGGAG	3 cut(s)	227, 2571, 3341
BseSI	GKGCMC	6 cut(s)	1486, 1654, 1858, 2048, 2476, 3257
BseYI	CCCAGC	1 cut(s)	2228
BsgI	GTGCAG	2 cut(s)	5, 3360
Bsh1236I	CGCG	1 cut(s)	38
Bsh1285I	CGRYCG	2 cut(s)	680, 3539
BshFI	GGCC	6 cut(s)	891, 1182, 1362, 1521, 2439, 3222
BshNI	GGYRCC	3 cut(s)	346, 1569, 2671
BshTI	ACCGGT	1 cut(s)	837
BsiEI	CGRYCG	2 cut(s)	680, 3539
BsiHKAI	GWGCWC	3 cut(s)	2048, 2299, 2476
BsiSI	CCGG	2 cut(s)	838, 1179
BslFI	GGGAC	4 cut(s)	129, 728, 2129, 2675
BslI	CCNNNNNNNGG	6 cut(s)	132, 133, 243, 494, 641, 2541
BsmAI	GTCTC	3 cut(s)	1089, 2038, 2582
BsmFI	GGGAC	4 cut(s)	129, 728, 2129, 2675
BsmI	GAATGC	1 cut(s)	529
BsnI	GGCC	6 cut(s)	891, 1182, 1362, 1521, 2439, 3222
Bso31I	GGTCTC	1 cut(s)	2038
Bsp1286I	GDGCHC	7 cut(s)	1486, 1654, 1858, 2048, 2299, 2476, 3257
Bsp1407I	TGTACA	1 cut(s)	380
Bsp143I	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
BspACI	CCGC	9 cut(s)	36, 195, 369, 676, 1024, 2585, 3466, 3482, 3539
BspANI	GGCC	6 cut(s)	891, 1182, 1362, 1521, 2439, 3222
BspCNI	CTCAG	7 cut(s)	934, 1479, 1966, 1996, 2638, 3109, 3453
BspFNI	CGCG	1 cut(s)	38
BspHI	TCATGA	2 cut(s)	1123, 1282
BspLI	GGNNCC	6 cut(s)	348, 695, 1571, 1970, 2673, 3478
BspMAI	CTGCAG	2 cut(s)	2611, 2641
BspPI	GGATC	5 cut(s)	279, 337, 1963, 1976, 3401
BspT107I	GGYRCC	3 cut(s)	346, 1569, 2671
BspTNI	GGTCTC	1 cut(s)	2038
BsrBI	CCGCTC	3 cut(s)	197, 369, 3466
BsrDI	GCAATG	2 cut(s)	1098, 2440
BsrFI	RCCGGY	2 cut(s)	837, 1178
BsrGI	TGTACA	1 cut(s)	380
BsrI	ACTGG	3 cut(s)	390, 975, 1661
BssAI	RCCGGY	2 cut(s)	837, 1178
BssMI	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
BssNI	GRCGYC	1 cut(s)	1570
BssT1I	CCWWGG	3 cut(s)	2166, 2631, 3223
Bst6I	CTCTTC	5 cut(s)	34, 559, 612, 2208, 2854
BstACI	GRCGYC	1 cut(s)	1570
BstAPI	GCANNNNNTGC	4 cut(s)	19, 2606, 3133, 3347
BstAUI	TGTACA	1 cut(s)	380
BstBAI	YACGTR	2 cut(s)	803, 2492
BstC8I	GCNNGC	7 cut(s)	11, 15, 59, 1180, 1215, 1662, 2911
BstFNI	CGCG	1 cut(s)	38
BstH2I	RGCGCY	2 cut(s)	1573, 3439
BstHHI	GCGC	5 cut(s)	19, 40, 1572, 2702, 3438
BstKTI	GATC	9 cut(s)	177, 274, 332, 763, 808, 1192, 1453, 1971, 3409
BstMAI	GTCTC	3 cut(s)	1089, 2038, 2582
BstMBI	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
BstMCI	CGRYCG	2 cut(s)	680, 3539
BstNSI	RCATGY	4 cut(s)	17, 941, 2328, 2913
BstSFI	CTRYAG	8 cut(s)	591, 985, 1200, 2607, 2637, 2855, 3375, 3382
BstSLI	GKGCMC	6 cut(s)	1486, 1654, 1858, 2048, 2476, 3257
BstUI	CGCG	1 cut(s)	38
BstX2I	RGATCY	2 cut(s)	1189, 1968
BstYI	RGATCY	2 cut(s)	1189, 1968
BsuI	GTATCC	1 cut(s)	1399
BsuRI	GGCC	6 cut(s)	891, 1182, 1362, 1521, 2439, 3222
BtsI	GCAGTG	2 cut(s)	1554, 2709
BtsIMutI	CAGTG	9 cut(s)	383, 600, 1446, 1554, 1654, 2454, 2709, 3328, 3453
Cac8I	GCNNGC	7 cut(s)	11, 15, 59, 1180, 1215, 1662, 2911
CaiI	CAGNNNCTG	6 cut(s)	599, 2114, 2228, 2645, 2797, 2966
CciI	TCATGA	2 cut(s)	1123, 1282
CfoI	GCGC	5 cut(s)	19, 40, 1572, 2702, 3438
Cfr10I	RCCGGY	2 cut(s)	837, 1178
Cfr13I	GGNCC	5 cut(s)	835, 1181, 1703, 2174, 2504
CseI	GACGC	1 cut(s)	1547
Csp6I	GTAC	8 cut(s)	381, 800, 1013, 2073, 2252, 2672, 3296, 3444
CspAI	ACCGGT	1 cut(s)	837
CviQI	GTAC	8 cut(s)	381, 800, 1013, 2073, 2252, 2672, 3296, 3444
DinI	GGCGCC	1 cut(s)	1571
DpnI	GATC	9 cut(s)	176, 273, 331, 762, 807, 1191, 1452, 1970, 3408
DpnII	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
DraI	TTTAAA	2 cut(s)	873, 2058
EaeI	YGGCCR	1 cut(s)	2437
Eam1104I	CTCTTC	5 cut(s)	34, 559, 612, 2208, 2854
EarI	CTCTTC	5 cut(s)	34, 559, 612, 2208, 2854
Ecl136II	GAGCTC	1 cut(s)	2297
Eco130I	CCWWGG	3 cut(s)	2166, 2631, 3223
Eco24I	GRGCYC	1 cut(s)	2299
Eco31I	GGTCTC	1 cut(s)	2038
Eco32I	GATATC	2 cut(s)	1117, 3517
Eco47I	GGWCC	4 cut(s)	835, 1703, 2174, 2504
Eco53kI	GAGCTC	1 cut(s)	2297
Eco57I	CTGAAG	8 cut(s)	636, 764, 1206, 1782, 1920, 1932, 2019, 3225
Eco72I	CACGTG	1 cut(s)	2492
EcoICRI	GAGCTC	1 cut(s)	2297
EcoRV	GATATC	2 cut(s)	1117, 3517
EcoT14I	CCWWGG	3 cut(s)	2166, 2631, 3223
EcoT22I	ATGCAT	1 cut(s)	2911
EcoT38I	GRGCYC	1 cut(s)	2299
EgeI	GGCGCC	1 cut(s)	1571
EheI	GGCGCC	1 cut(s)	1571
ErhI	CCWWGG	3 cut(s)	2166, 2631, 3223
FaqI	GGGAC	4 cut(s)	129, 728, 2129, 2675
FauI	CCCGC	1 cut(s)	3489
FauNDI	CATATG	1 cut(s)	1993
FblI	GTMKAC	1 cut(s)	3378
FriOI	GRGCYC	1 cut(s)	2299
FspBI	CTAG	6 cut(s)	630, 1193, 2067, 2774, 3249, 3332
GlaI	GCGC	5 cut(s)	18, 39, 1571, 2701, 3437
GsaI	CCCAGC	1 cut(s)	2232
GsuI	CTGGAG	5 cut(s)	407, 1257, 1406, 2574, 2819
HaeII	RGCGCY	2 cut(s)	1573, 3439
HaeIII	GGCC	6 cut(s)	891, 1182, 1362, 1521, 2439, 3222
HapII	CCGG	2 cut(s)	838, 1179
HgaI	GACGC	1 cut(s)	1547
HhaI	GCGC	5 cut(s)	19, 40, 1572, 2702, 3438
Hin1I	GRCGYC	1 cut(s)	1570
Hin6I	GCGC	5 cut(s)	17, 38, 1570, 2700, 3436
HinP1I	GCGC	5 cut(s)	17, 38, 1570, 2700, 3436
HincII	GTYRAC	2 cut(s)	2194, 3399
HindII	GTYRAC	2 cut(s)	2194, 3399
HindIII	AAGCTT	3 cut(s)	788, 1163, 1959
HpaI	GTTAAC	2 cut(s)	2194, 3399
HpaII	CCGG	2 cut(s)	838, 1179
Hpy166II	GTNNAC	9 cut(s)	383, 548, 1065, 1706, 2046, 2194, 2474, 3379, 3399
Hpy8I	GTNNAC	9 cut(s)	383, 548, 1065, 1706, 2046, 2194, 2474, 3379, 3399
Hpy99I	CGWCG	4 cut(s)	57, 113, 2669, 3081
HpyCH4IV	ACGT	2 cut(s)	802, 2491
HpySE526I	ACGT	2 cut(s)	802, 2491
Hsp92I	GRCGYC	1 cut(s)	1570
HspAI	GCGC	5 cut(s)	17, 38, 1570, 2700, 3436
KasI	GGCGCC	1 cut(s)	1569
KpnI	GGTACC	1 cut(s)	2675
KroI	GCCGGC	1 cut(s)	1178
KroNI	GCCGGC	1 cut(s)	1180
KspAI	GTTAAC	2 cut(s)	2194, 3399
Kzo9I	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
LmnI	GCTCC	6 cut(s)	1250, 1540, 2111, 2838, 3328, 3482
MaeI	CTAG	6 cut(s)	630, 1193, 2067, 2774, 3249, 3332
MaeII	ACGT	2 cut(s)	802, 2491
MaeIII	GTNAC	5 cut(s)	464, 2218, 2234, 2320, 2769
MalI	GATC	9 cut(s)	176, 273, 331, 762, 807, 1191, 1452, 1970, 3408
MbiI	CCGCTC	3 cut(s)	197, 369, 3466
MboI	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
MfeI	CAATTG	2 cut(s)	204, 2178
MflI	RGATCY	2 cut(s)	1189, 1968
MhlI	GDGCHC	7 cut(s)	1486, 1654, 1858, 2048, 2299, 2476, 3257
MlsI	TGGCCA	1 cut(s)	2439
MluNI	TGGCCA	1 cut(s)	2439
Mly113I	GGCGCC	1 cut(s)	1570
MlyI	GAGTC	5 cut(s)	248, 413, 635, 1337, 2972
MmeI	TCCRAC	5 cut(s)	186, 796, 822, 1733, 3032
Mox20I	TGGCCA	1 cut(s)	2439
Mph1103I	ATGCAT	1 cut(s)	2911
MroNI	GCCGGC	1 cut(s)	1178
MroXI	GAANNNNTTC	1 cut(s)	1917
MscI	TGGCCA	1 cut(s)	2439
MseI	TTAA	9 cut(s)	258, 872, 1143, 1613, 1626, 1866, 2057, 2193, 3398
MslI	CAYNNNNRTG	4 cut(s)	522, 1110, 1173, 2286
Msp20I	TGGCCA	1 cut(s)	2439
MspA1I	CMGCKG	2 cut(s)	2225, 2963
MspI	CCGG	2 cut(s)	838, 1179
MunI	CAATTG	2 cut(s)	204, 2178
Mva1269I	GAATGC	1 cut(s)	529
MvnI	CGCG	1 cut(s)	38
NaeI	GCCGGC	1 cut(s)	1180
NarI	GGCGCC	1 cut(s)	1570
NdeI	CATATG	1 cut(s)	1993
NdeII	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
NgoMIV	GCCGGC	1 cut(s)	1178
NlaIV	GGNNCC	6 cut(s)	348, 695, 1571, 1970, 2673, 3478
NmeAIII	GCCGAG	1 cut(s)	61
NmuCI	GTSAC	2 cut(s)	464, 2320
NsiI	ATGCAT	1 cut(s)	2911
NspI	RCATGY	4 cut(s)	17, 941, 2328, 2913
PaeI	GCATGC	2 cut(s)	17, 2913
PagI	TCATGA	2 cut(s)	1123, 1282
PasI	CCCWGGG	2 cut(s)	2147, 2478
PciI	ACATGT	1 cut(s)	2324
PcsI	WCGNNNNNNNCGW	1 cut(s)	931
PctI	GAATGC	1 cut(s)	529
PdiI	GCCGGC	1 cut(s)	1180
PdmI	GAANNNNTTC	1 cut(s)	1917
PfeI	GAWTC	8 cut(s)	895, 1331, 1620, 1729, 2008, 2455, 2642, 3453
PfoI	TCCNGGA	1 cut(s)	2835
PinAI	ACCGGT	1 cut(s)	837
PleI	GAGTC	5 cut(s)	247, 412, 634, 1337, 2972
PluTI	GGCGCC	1 cut(s)	1573
PmaCI	CACGTG	1 cut(s)	2492
PmlI	CACGTG	1 cut(s)	2492
PpsI	GAGTC	5 cut(s)	247, 412, 634, 1337, 2972
Ppu21I	YACGTR	2 cut(s)	803, 2492
PscI	ACATGT	1 cut(s)	2324
PsiI	TTATAA	1 cut(s)	2658
Psp124BI	GAGCTC	1 cut(s)	2299
PspCI	CACGTG	1 cut(s)	2492
PspFI	CCCAGC	1 cut(s)	2228
PspN4I	GGNNCC	6 cut(s)	348, 695, 1571, 1970, 2673, 3478
PspPI	GGNCC	5 cut(s)	835, 1181, 1703, 2174, 2504
PsrI	GAACNNNNNNTAC	2 cut(s)	2065, 2097
PstI	CTGCAG	2 cut(s)	2611, 2641
PstNI	CAGNNNCTG	6 cut(s)	599, 2114, 2228, 2645, 2797, 2966
PsuI	RGATCY	2 cut(s)	1189, 1968
PvuII	CAGCTG	2 cut(s)	2225, 2963
RsaI	GTAC	8 cut(s)	382, 801, 1014, 2074, 2253, 2673, 3297, 3445
RsaNI	GTAC	8 cut(s)	381, 800, 1013, 2073, 2252, 2672, 3296, 3444
RseI	CAYNNNNRTG	4 cut(s)	522, 1110, 1173, 2286
SacI	GAGCTC	1 cut(s)	2299
SaqAI	TTAA	9 cut(s)	258, 872, 1143, 1613, 1626, 1866, 2057, 2193, 3398
Sau3AI	GATC	9 cut(s)	174, 271, 329, 760, 805, 1189, 1450, 1968, 3406
Sau96I	GGNCC	5 cut(s)	835, 1181, 1703, 2174, 2504
ScaI	AGTACT	1 cut(s)	1014
SchI	GAGTC	5 cut(s)	248, 413, 635, 1337, 2972
SduI	GDGCHC	7 cut(s)	1486, 1654, 1858, 2048, 2299, 2476, 3257
SfcI	CTRYAG	8 cut(s)	591, 985, 1200, 2607, 2637, 2855, 3375, 3382
SfoI	GGCGCC	1 cut(s)	1571
SinI	GGWCC	4 cut(s)	835, 1703, 2174, 2504
SmiMI	CAYNNNNRTG	4 cut(s)	522, 1110, 1173, 2286
SmlI	CTYRAG	5 cut(s)	1130, 1289, 1589, 2085, 3461
SmoI	CTYRAG	5 cut(s)	1130, 1289, 1589, 2085, 3461
SphI	GCATGC	2 cut(s)	17, 2913
SsiI	CCGC	9 cut(s)	36, 195, 369, 676, 1024, 2585, 3466, 3482, 3539
SspDI	GGCGCC	1 cut(s)	1569
SspI	AATATT	6 cut(s)	262, 306, 1907, 1929, 2025, 2895
SspMI	CTAG	6 cut(s)	630, 1193, 2067, 2774, 3249, 3332
SstI	GAGCTC	1 cut(s)	2299
StyI	CCWWGG	3 cut(s)	2166, 2631, 3223
TaiI	ACGT	2 cut(s)	805, 2494
TaqI	TCGA	7 cut(s)	265, 680, 763, 925, 964, 2299, 3079
TatI	WGTACW	4 cut(s)	380, 1012, 2251, 3443
TfiI	GAWTC	8 cut(s)	895, 1331, 1620, 1729, 2008, 2455, 2642, 3453
Tru1I	TTAA	9 cut(s)	258, 872, 1143, 1613, 1626, 1866, 2057, 2193, 3398
Tru9I	TTAA	9 cut(s)	258, 872, 1143, 1613, 1626, 1866, 2057, 2193, 3398
TscAI	CASTG	9 cut(s)	390, 600, 1453, 1554, 1661, 2454, 2709, 3328, 3453
TseFI	GTSAC	2 cut(s)	464, 2320
Tsp45I	GTSAC	2 cut(s)	464, 2320
TspGWI	ACGGA	3 cut(s)	548, 3194, 3407
TspRI	CASTG	9 cut(s)	390, 600, 1453, 1554, 1661, 2454, 2709, 3328, 3453
VneI	GTGCAC	2 cut(s)	2044, 2472
VpaK11BI	GGWCC	4 cut(s)	835, 1703, 2174, 2504
XapI	RAATTY	6 cut(s)	284, 856, 978, 1029, 3281, 3316
XceI	RCATGY	4 cut(s)	17, 941, 2328, 2913
XcmI	CCANNNNNNNNNTGG	1 cut(s)	284
XmiI	GTMKAC	1 cut(s)	3378
XmnI	GAANNNNTTC	1 cut(s)	1917
XspI	CTAG	6 cut(s)	630, 1193, 2067, 2774, 3249, 3332
ZrmI	AGTACT	1 cut(s)	1014
Zsp2I	ATGCAT	1 cut(s)	2911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.