Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008467

Physical Location & Seq

Genomic Coordinates Forward (+)

35067831 .. 35080462

Gene Structure 12632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00355910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3627 bp

ATGGATTGCGTGCCTTCGCTGAACACCTCCGCTTCTTCTTCTTCACAGTTCCTCCGTCACAAGCAATTCAGATTCCTCCACAATCACCTTCAATGCCGTCGAAGCGTTGCGGCACTCGGCCGTAGAACAAGCTTCCGTCCTCTCCATCACAAATCAAACAATCTTCGGATTCTCTGCGGCGTCGGTGTTTCTCCTCCTCAATCCACAGAGGAGCAGAAGGAGACCAAGATGAAGTCCAAGTCAAAGTCTGGGCGCGGCAAGGTTTGGCTTGATATTAGGCTGGATCACCAAGTTGCGTTTGGAGAGAGTGTTGCGGTTTTGGGATCATCAAAGGAGTTAGGGTCGTGGAAGAAGAAGGTGCCTCTGAATTGGACAGAGAGTGGATGGGTATGCAAATTGGAGTTCAAGGGGGGCGAGTCCGTTGAGTATAAGTTTGTAACCGTTAGGGCTGACAAGAGTATGCTTTGGGAAGGTGGTGATAACCGGGCTTTGAAACTTCCCAACGGAGGAAGCTTTGGGATGGTTTCCCACTGGGATGCAACTGGGGAGAATGTAGATCTATTCCCATTGGACAAGGAGGATGGTGTGGAACATAAGGGGTCTTCTGTTGCCCAAACTGCTAGTACAACTGCTAGTTCTCCAGATGTGGAGACTAGTCCTTTTGTTGGTCAATGGAAAGGGAATGCCATCTCATTTATGCGGTCAAATGAGCATCGGGATCGTGAATCAGAAAGAAATTGGGACACTTCAGGTCTTGAAGGGTTGTCTCTGAAGTTGGTTGAAGGTGATCGAAATGCCAGGAATTGGTGGCAAAAGCTTGAAGTTGTACGTGATATACTACTTGAAAGTTCGCAAAGTGAGGAGCGGTTGGATGCTCTCATAAATTCTTCCATTTATTTGAAGGTCTACTTCGCTATTCTTTTCCATTTTTTTCTATTTTGGTTTCTTGCTTGCATTGCTTATATCTCCTTCTTAATGCAGTGGATAAACACAGGCCAGATTCCTTGCTTTGAAGGTGGAGGTCATCATCGACCAAACCGGCATGCTGAGATTTCCAGGGTTATATTTCGAGAACTGGAAAGAATTTCCTGTAGGAAAGATACTTCACCCCAGGAAGTACTTGTTATCCGCAAAATTCATCCATGTCTGCCATCATTCAAGGCAGAATTTACTGCATCTGTCCCACTAACACGAATCAGAGACATTGCTCATCGGAATGATATCCCTCATGACCTCAAGCAAGAAATTAAGCACACAATACAAAACAAACTTCACCGCAATGCTGGTCCTGAAGATCTAGTTGCTACAGAAGCCATGCTAGCAAGAATAACCGAGAACCCTGGACAATATAGTGAAGCATTTGTGGAGCAGTTCAAGATTTTCCATCATGAACTCAAAGACTTTTTCAATGCTGGAAGCCTTGCTGAACAACTTGAATCCATCAAAGATTCCATCGATGATAAAGGCCGTTCAGCTCTTACATTGTTTTTGGAGTGCAAAAAGGGTTTGGATGCTTCAGCAGAATCAAGTAAAGTTTTGGGATCTGATTTGTTGTTCAAGACCATGCAATCCTTGAGTGCTCTGAGAGAAATAATTGCAAAGGGTCTTGAAAGTGGCCTTAGAAATGATGCTTCTGATGCTGCTATAGCTATGCGTCAAATGTTGACAATCATTGACTTGGTTAGATTCGCTAATGAGCTTGAAGCTATGGGAGGGGCTCATTGGCTGGCACAAGATGTGAAATCAAAGGATGTAAGCTCTTGGAATGATCCACTTGGTGCTCTTGTAGTTGGTGTTCATCAGCTAAGGTTATCTGGTTGGAAGCCTGAAGAATGTGCTGCTATTCAAAACGAGCTCCTTGCCTGGAAAGCAATAGGCCTTTCTGAAACAGAAGCGAGTGAAGATGGCAAAACAATCTGGGGACTACGGCATAAAGCTACTCTCGATAGAGCCAGGAGGTTAACTGAAGAATATTCTGAAGCACTTCTTCAAATATTTCCCCAAAATGTCCAGGTATTAGGAAAAGCTTTTGGAATACCTGAGAACAGTGTAAGGACATACGCTGAAGCTGAAATTCGGGCTGGTGTAATATTTCAGGTTTCAAAACTCTGTACCCTACTTTTGAAAGCAGTTAGAACTACCATTGGTTCTCAGGGTTGGGATGTTATTGTGCCGGGAGCTGCTATGGGAACATTAGTTCAGGTTGAGAGGATTGTCCCCGGTTCAATACCATCAACTGTGGAGGGACCTGTTGTTCTTGTTGTCAACAAAGCAGATGGAGATGAAGAGGTAACAGCGGCTGGGAGTAACATTGTGGGAGTTGTTCTTCTGCAAGAGCTTCCTCACTTGTCTCATCTCGGTGTCAGGGCTCGACAAGAGAAGGTTGTGTTTGTGACATGTGAAGATGATGACAAAGTTGCTGATATACAAAAACTCATGGGAAAATATGTGAGGTTAGAAGCATCCTCGTCCAGTGTTGATATATATCCTTCATCAGAAAACAGCAGTGGCACTTTGGCAGTGAAAAATGTTTCAAGTGATGTTGCACCAAAATTTGAATCCCGTGGAACTCCTGACCATTCCTGGTCTGCTGCTAAAACCCCAGAATCCAATCAGGGGGTATCTGCCGGTGGAGTTTTACTACTTGCTGATGCAGAAGCACGGAATTCGGGTGCAAAAGCTGCTGCTTGTGGTCGTTTAGCTTCTTTGGCAGCAGCATCTGATAAAGTTTTTAGTGACCAAGGGGTTCCAGCTTCCTTTAATGTCCCTGCTGGAGCAGTGATACCATTTGGCTCTATGGAACTGGCATTAGAGCAAAGCAAATCCATGGAATCATTCAGGTCCTTTCTAAATAAGATAGAAACATTGAAGCCAGAAAGTGGAGAACTTGACCAAGTATGTGGTCAGCTCCAGGAACTGATCTCTTCTCTACAGCCCTCCAAAGATATCATTGATATAATAGCCAAAATATTTCCAGGCAATGCGCGGCTAATTGTTCGTTCAAGTGCTAATGTTGAGGACTTAGCCGGAATGTCGGCTGCTGGACTATATGATTCAATTCCCAATGTTAGTGTTTCAAATCCGACAGTATTTGCAAATTCTATAAGCAGAGTGTGGGCATCACTTTATACTAGGAGGGCAGTTTTGAGCCGTCGAGTTGCCGGTGTACCCCAGAAGGATGCTACAATGGCGATTCTGGTGCAAGAAATGCTTTCACCAGACTTATCGTTTGTGCTTCATACAGTCAGCCCAACAGACCAAGACCATAATTTGGTTGAGGCTGAAATTGCTTCTGGCCTCGGTGAAACTTTGGCTTCAGGCACCAGGGGCACACCCTGGCGTATATCGTCTGGAAAATTTGATGGGAATGTGCGCACATTGGCATTTGCTAATTTCAGTGAGGAACTACTTGGTGCAGGCCCTGCAGACGGGGAGGTTATCCATTTGACTGTAGACTACAGCAAGAAACCATTAACTGTTGATCCAGTTTTCCGTCGGCAGCTTGGTCAGCGCCTCGGTGCGGTGGGATTTTTCCTTGAGCAGAAGTTTGGCTGCCCACAGGATGTGGAAGGATGTGTTGTTGGCAAAGACATCTTTATAGTCCAGACACGTCCACAGCCCCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0019200 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046835 GO:0051752 GO:0071704 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 PPDK_N

Protein Analysis

1208

Amino Acids

132.39

Weight (kDa)

6.23

Isoelectric Point (pI)

40.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	94 - 171	6.3e-15	Starch binding domain
GWD1_pHisD	PF22973	720 - 834	2.4e-34	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	919 - 1206	1.4e-30	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26570 AT5G26570
fragaria_vesca	FvH4_4g12510 FvH4_4g12510
malus_domestica	MD04G1025900.v1.1
prunus_persica	Prupe.1G175200_v2.0.a1
pyrus_communis	pycom04g02200
rosa_chinensis	RchiOBHm_Chr4g0411251 RchiOBHm_Chr4g0411331
rosa_laevigata	RLG00000008365 RLG00000008368
rosa_multiflora	Rmu_sc0000543.1_g000027 Rmu_sc0000543.1_g000049
rosa_roxburghii	Rroxscaffold_5G00355910
rosa_rugosa	Rorug04G0110100
rosa_samantha	Rh4AG167100 Rh4BG163600 Rh4BG164200 Rh4CG178800 Rh4DG161900
rosa_wichuraiana	Rw4G013760 Rw4G013810 Rw4G013910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3374
AccB1I	GGYRCC	2 cut(s)	358, 3320
AccB7I	CCANNNNNTGG	3 cut(s)	804, 2879, 3271
AccBSI	CCGCTC	1 cut(s)	865
AccI	GTMKAC	2 cut(s)	906, 3453
AccII	CGCG	2 cut(s)	255, 2986
AclWI	GGATC	6 cut(s)	291, 331, 726, 1549, 1763, 3476
AcoI	YGGCCR	1 cut(s)	118
AcsI	RAATTY	9 cut(s)	883, 1083, 1134, 1166, 2073, 2552, 2665, 3097, 3356
AcuI	CTGAAG	9 cut(s)	732, 791, 1311, 1500, 1848, 1986, 1998, 2085, 3300
AcyI	GRCGYC	1 cut(s)	180
AdeI	CACNNNGTG	1 cut(s)	1778
AfaI	GTAC	5 cut(s)	625, 828, 1119, 2113, 3168
AfiI	CCNNNNNNNGG	8 cut(s)	506, 665, 804, 2616, 2879, 3271, 3428, 3520
AflIII	ACRYGT	2 cut(s)	2396, 3608
AhlI	ACTAGT	1 cut(s)	653
AjiI	CACGTC	1 cut(s)	3611
AloI	GAACNNNNNNTCC	4 cut(s)	2181, 2213, 2238, 2270
Alw21I	GWGCWC	3 cut(s)	1582, 1783, 1857
Alw26I	GTCTC	5 cut(s)	215, 644, 771, 1194, 2355
AlwI	GGATC	6 cut(s)	291, 331, 726, 1549, 1763, 3476
AlwNI	CAGNNNCTG	4 cut(s)	2300, 2720, 2917, 3422
AoxI	GGCC	7 cut(s)	118, 994, 1465, 1615, 1876, 3295, 3418
ApoI	RAATTY	9 cut(s)	883, 1083, 1134, 1166, 2073, 2552, 2665, 3097, 3356
ArsI	GACNNNNNNTTYG	2 cut(s)	3212, 3244
Asp700I	GAANNNNTTC	1 cut(s)	1983
AspLEI	GCGC	4 cut(s)	255, 2986, 3375, 3513
AspS9I	GGNCC	4 cut(s)	1286, 2246, 2841, 3419
AsuC2I	CCSGG	3 cut(s)	485, 2175, 2220
AsuHPI	GGTGA	8 cut(s)	77, 278, 488, 797, 1098, 1265, 3207, 3314
AsuNHI	GCTAGC	1 cut(s)	1318
AvaII	GGWCC	3 cut(s)	1286, 2246, 2841
BaeGI	GKGCMC	1 cut(s)	3332
BanI	GGYRCC	2 cut(s)	358, 3320
BanII	GRGCYC	3 cut(s)	1720, 1857, 2371
BbsI	GAAGAC	1 cut(s)	594
Bbv12I	GWGCWC	3 cut(s)	1582, 1783, 1857
BceAI	ACGGC	5 cut(s)	81, 105, 1452, 1943, 3135
BcgI	CGANNNNNNTGC	8 cut(s)	701, 735, 1841, 1875, 2649, 2683, 3181, 3215
BcnI	CCSGG	3 cut(s)	485, 2175, 2220
BcoDI	GTCTC	5 cut(s)	215, 644, 771, 1194, 2355
BcuI	ACTAGT	1 cut(s)	653
BfaI	CTAG	6 cut(s)	621, 633, 654, 1298, 1319, 3132
BfmI	CTRYAG	7 cut(s)	1090, 1305, 1644, 2930, 3423, 3450, 3457
BfoI	RGCGCY	1 cut(s)	3514
BglII	AGATCT	2 cut(s)	556, 1294
BmcAI	AGTACT	1 cut(s)	1119
Bme18I	GGWCC	3 cut(s)	1286, 2246, 2841
BmgBI	CACGTC	1 cut(s)	3611
BmgT120I	GGNCC	4 cut(s)	1286, 2246, 2841, 3419
BmiI	GGNNCC	4 cut(s)	360, 2247, 2748, 3322
BmrI	ACTGGG	2 cut(s)	541, 552
BmtI	GCTAGC	1 cut(s)	1322
BmuI	ACTGGG	2 cut(s)	541, 552
BpiI	GAAGAC	1 cut(s)	594
BpmI	CTGGAG	3 cut(s)	624, 2793, 2894
Bpu10I	CCTNAGC	1 cut(s)	1805
BpuEI	CTTGAG	3 cut(s)	1220, 1594, 3557
BpuMI	CCSGG	3 cut(s)	485, 2175, 2220
Bsa29I	ATCGAT	1 cut(s)	1455
BsaAI	YACGTR	1 cut(s)	830
BsaBI	GATNNNNATC	1 cut(s)	2484
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	215
BsaXI	ACNNNNNCTCC	6 cut(s)	36, 66, 294, 324, 326, 356
Bsc4I	CCNNNNNNNGG	8 cut(s)	506, 665, 804, 2616, 2879, 3271, 3428, 3520
Bse118I	RCCGGY	3 cut(s)	1038, 2627, 3161
Bse1I	ACTGG	6 cut(s)	536, 547, 1080, 2472, 2808, 3485
Bse3DI	GCAATG	4 cut(s)	954, 1203, 1285, 2986
Bse8I	GATNNNNATC	1 cut(s)	2484
BseCI	ATCGAT	1 cut(s)	1455
BseJI	GATNNNNATC	1 cut(s)	2484
BseLI	CCNNNNNNNGG	8 cut(s)	506, 665, 804, 2616, 2879, 3271, 3428, 3520
BseMI	GCAATG	4 cut(s)	954, 1203, 1285, 2986
BseMII	CTCAG	4 cut(s)	1038, 1574, 2031, 2165
BseNI	ACTGG	6 cut(s)	536, 547, 1080, 2472, 2808, 3485
BseRI	GAGGAG	4 cut(s)	183, 186, 224, 875
BseSI	GKGCMC	1 cut(s)	3332
BseX3I	CGGCCG	1 cut(s)	118
BseYI	CCCAGC	1 cut(s)	2300
BsgI	GTGCAG	1 cut(s)	3435
Bsh1236I	CGCG	2 cut(s)	255, 2986
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	7 cut(s)	120, 996, 1467, 1617, 1878, 3297, 3420
BshNI	GGYRCC	2 cut(s)	358, 3320
BshVI	ATCGAT	1 cut(s)	1455
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	3 cut(s)	1582, 1783, 1857
BsiSI	CCGG	7 cut(s)	484, 1039, 2174, 2220, 2628, 3027, 3162
BslFI	GGGAC	6 cut(s)	755, 1166, 1935, 2201, 2259, 2750
BslI	CCNNNNNNNGG	8 cut(s)	506, 665, 804, 2616, 2879, 3271, 3428, 3520
BsmAI	GTCTC	5 cut(s)	215, 644, 771, 1194, 2355
BsmFI	GGGAC	6 cut(s)	755, 1166, 1935, 2201, 2259, 2750
BsmI	GAATGC	1 cut(s)	688
BsnI	GGCC	7 cut(s)	120, 996, 1467, 1617, 1878, 3297, 3420
Bso31I	GGTCTC	1 cut(s)	215
Bsp1286I	GDGCHC	6 cut(s)	1582, 1720, 1783, 1857, 2371, 3332
Bsp19I	CCATGG	1 cut(s)	2826
BspANI	GGCC	7 cut(s)	120, 996, 1467, 1617, 1878, 3297, 3420
BspCNI	CTCAG	4 cut(s)	1039, 1575, 2032, 2164
BspDI	ATCGAT	1 cut(s)	1455
BspFNI	CGCG	2 cut(s)	255, 2986
BspHI	TCATGA	2 cut(s)	1228, 1387
BspLI	GGNNCC	4 cut(s)	360, 2247, 2748, 3322
BspMAI	CTGCAG	1 cut(s)	3427
BspOI	GCTAGC	1 cut(s)	1322
BspPI	GGATC	6 cut(s)	291, 331, 726, 1549, 1763, 3476
BspT107I	GGYRCC	2 cut(s)	358, 3320
BspTNI	GGTCTC	1 cut(s)	215
BsrBI	CCGCTC	1 cut(s)	865
BsrDI	GCAATG	4 cut(s)	954, 1203, 1285, 2986
BsrFI	RCCGGY	3 cut(s)	1038, 2627, 3161
BsrI	ACTGG	6 cut(s)	536, 547, 1080, 2472, 2808, 3485
BssAI	RCCGGY	3 cut(s)	1038, 2627, 3161
BssNI	GRCGYC	1 cut(s)	180
BssT1I	CCWWGG	2 cut(s)	2740, 2826
Bst4CI	ACNGT	8 cut(s)	48, 442, 2048, 2239, 3088, 3244, 3451, 3478
Bst6I	CTCTTC	2 cut(s)	2280, 2929
BstACI	GRCGYC	1 cut(s)	180
BstAPI	GCANNNNNTGC	3 cut(s)	2681, 3208, 3422
BstBAI	YACGTR	1 cut(s)	830
BstC8I	GCNNGC	6 cut(s)	11, 952, 1044, 1320, 1728, 3418
BstDEI	CTNAG	7 cut(s)	1047, 1583, 1619, 1805, 2040, 2151, 3022
BstDSI	CCRYGG	2 cut(s)	2563, 2826
BstFNI	CGCG	2 cut(s)	255, 2986
BstH2I	RGCGCY	1 cut(s)	3514
BstHHI	GCGC	4 cut(s)	255, 2986, 3375, 3513
BstMAI	GTCTC	5 cut(s)	215, 644, 771, 1194, 2355
BstMCI	CGRYCG	1 cut(s)	121
BstNSI	RCATGY	2 cut(s)	1046, 2400
BstSFI	CTRYAG	7 cut(s)	1090, 1305, 1644, 2930, 3423, 3450, 3457
BstSLI	GKGCMC	1 cut(s)	3332
BstUI	CGCG	2 cut(s)	255, 2986
BstV2I	GAAGAC	1 cut(s)	594
BstX2I	RGATCY	3 cut(s)	556, 1294, 1541
BstXI	CCANNNNNNTGG	1 cut(s)	2900
BstYI	RGATCY	3 cut(s)	556, 1294, 1541
BstZI	CGGCCG	1 cut(s)	118
Bsu15I	ATCGAT	1 cut(s)	1455
BsuRI	GGCC	7 cut(s)	120, 996, 1467, 1617, 1878, 3297, 3420
BsuTUI	ATCGAT	1 cut(s)	1455
BtgI	CCRYGG	2 cut(s)	2563, 2826
BtrI	CACGTC	1 cut(s)	3611
BtsI	GCAGTG	4 cut(s)	986, 2512, 2526, 2784
BtsIMutI	CAGTG	8 cut(s)	529, 986, 2053, 2479, 2512, 2526, 2784, 3403
Cac8I	GCNNGC	6 cut(s)	11, 952, 1044, 1320, 1728, 3418
CaiI	CAGNNNCTG	4 cut(s)	2300, 2720, 2917, 3422
CciI	TCATGA	2 cut(s)	1228, 1387
CfoI	GCGC	4 cut(s)	255, 2986, 3375, 3513
Cfr10I	RCCGGY	3 cut(s)	1038, 2627, 3161
Cfr13I	GGNCC	4 cut(s)	1286, 2246, 2841, 3419
ClaI	ATCGAT	1 cut(s)	1455
CseI	GACGC	2 cut(s)	169, 1643
Csp6I	GTAC	5 cut(s)	624, 827, 1118, 2112, 3167
CviAII	CATG	9 cut(s)	1043, 1143, 1229, 1315, 1388, 1564, 2397, 2437, 2827
CviQI	GTAC	5 cut(s)	624, 827, 1118, 2112, 3167
DdeI	CTNAG	7 cut(s)	1047, 1583, 1619, 1805, 2040, 2151, 3022
DraIII	CACNNNGTG	1 cut(s)	1778
EaeI	YGGCCR	1 cut(s)	118
EagI	CGGCCG	1 cut(s)	118
Eam1104I	CTCTTC	2 cut(s)	2280, 2929
EarI	CTCTTC	2 cut(s)	2280, 2929
Ecl136II	GAGCTC	1 cut(s)	1855
EclXI	CGGCCG	1 cut(s)	118
Eco130I	CCWWGG	2 cut(s)	2740, 2826
Eco147I	AGGCCT	1 cut(s)	1878
Eco24I	GRGCYC	3 cut(s)	1720, 1857, 2371
Eco31I	GGTCTC	1 cut(s)	215
Eco32I	GATATC	2 cut(s)	1222, 2947
Eco47I	GGWCC	3 cut(s)	1286, 2246, 2841
Eco52I	CGGCCG	1 cut(s)	118
Eco53kI	GAGCTC	1 cut(s)	1855
Eco57I	CTGAAG	9 cut(s)	732, 791, 1311, 1500, 1848, 1986, 1998, 2085, 3300
EcoICRI	GAGCTC	1 cut(s)	1855
EcoO109I	RGGNCCY	3 cut(s)	2246, 2841, 3419
EcoRI	GAATTC	1 cut(s)	2665
EcoRV	GATATC	2 cut(s)	1222, 2947
EcoT14I	CCWWGG	2 cut(s)	2740, 2826
EcoT38I	GRGCYC	3 cut(s)	1720, 1857, 2371
ErhI	CCWWGG	2 cut(s)	2740, 2826
FaeI	CATG	9 cut(s)	1046, 1146, 1232, 1318, 1391, 1567, 2400, 2440, 2830
FalI	AAGNNNNNCTT	6 cut(s)	893, 925, 1971, 2003, 3195, 3227
FaqI	GGGAC	6 cut(s)	755, 1166, 1935, 2201, 2259, 2750
FatI	CATG	9 cut(s)	1042, 1142, 1228, 1314, 1387, 1563, 2396, 2436, 2826
FblI	GTMKAC	2 cut(s)	906, 3453
FriOI	GRGCYC	3 cut(s)	1720, 1857, 2371
FspAI	RTGCGCAY	1 cut(s)	3374
FspBI	CTAG	6 cut(s)	621, 633, 654, 1298, 1319, 3132
FspI	TGCGCA	1 cut(s)	3374
GlaI	GCGC	4 cut(s)	254, 2985, 3374, 3512
GsaI	CCCAGC	1 cut(s)	2304
GsuI	CTGGAG	3 cut(s)	624, 2793, 2894
HaeII	RGCGCY	1 cut(s)	3514
HaeIII	GGCC	7 cut(s)	120, 996, 1467, 1617, 1878, 3297, 3420
HapII	CCGG	7 cut(s)	484, 1039, 2174, 2220, 2628, 3027, 3162
HgaI	GACGC	2 cut(s)	169, 1643
HhaI	GCGC	4 cut(s)	255, 2986, 3375, 3513
Hin1I	GRCGYC	1 cut(s)	180
Hin1II	CATG	9 cut(s)	1046, 1146, 1232, 1318, 1391, 1567, 2400, 2440, 2830
Hin6I	GCGC	4 cut(s)	253, 2984, 3373, 3511
HinP1I	GCGC	4 cut(s)	253, 2984, 3373, 3511
HincII	GTYRAC	3 cut(s)	1665, 1962, 2266
HindII	GTYRAC	3 cut(s)	1665, 1962, 2266
HindIII	AAGCTT	4 cut(s)	130, 511, 815, 2025
HpaI	GTTAAC	1 cut(s)	1962
HpaII	CCGG	7 cut(s)	484, 1039, 2174, 2220, 2628, 3027, 3162
HphI	GGTGA	8 cut(s)	77, 278, 488, 797, 1098, 1265, 3207, 3314
Hpy166II	GTNNAC	7 cut(s)	907, 1665, 1962, 2266, 3167, 3454, 3614
Hpy8I	GTNNAC	7 cut(s)	907, 1665, 1962, 2266, 3167, 3454, 3614
Hpy99I	CGWCG	4 cut(s)	102, 185, 3156, 3498
HpyCH4III	ACNGT	8 cut(s)	48, 442, 2048, 2239, 3088, 3244, 3451, 3478
HpyCH4IV	ACGT	2 cut(s)	829, 3610
HpyF3I	CTNAG	7 cut(s)	1047, 1583, 1619, 1805, 2040, 2151, 3022
HpySE526I	ACGT	2 cut(s)	829, 3610
Hsp92I	GRCGYC	1 cut(s)	180
Hsp92II	CATG	9 cut(s)	1046, 1146, 1232, 1318, 1391, 1567, 2400, 2440, 2830
HspAI	GCGC	4 cut(s)	253, 2984, 3373, 3511
KspAI	GTTAAC	1 cut(s)	1962
LmnI	GCTCC	7 cut(s)	211, 862, 1366, 1860, 2177, 2774, 2913
MaeI	CTAG	6 cut(s)	621, 633, 654, 1298, 1319, 3132
MaeII	ACGT	2 cut(s)	829, 3610
MaeIII	GTNAC	6 cut(s)	56, 436, 2290, 2306, 2392, 2735
MbiI	CCGCTC	1 cut(s)	865
MflI	RGATCY	3 cut(s)	556, 1294, 1541
MhlI	GDGCHC	6 cut(s)	1582, 1720, 1783, 1857, 2371, 3332
MlyI	GAGTC	1 cut(s)	425
MmeI	TCCRAC	3 cut(s)	849, 1799, 3107
MroXI	GAANNNNTTC	1 cut(s)	1983
MseI	TTAA	5 cut(s)	974, 1248, 1961, 2760, 3473
MslI	CAYNNNNRTG	4 cut(s)	534, 1215, 1278, 2358
MspA1I	CMGCKG	1 cut(s)	2297
MspI	CCGG	7 cut(s)	484, 1039, 2174, 2220, 2628, 3027, 3162
Mva1269I	GAATGC	1 cut(s)	688
MvnI	CGCG	2 cut(s)	255, 2986
NciI	CCSGG	3 cut(s)	485, 2175, 2220
NcoI	CCATGG	1 cut(s)	2826
NheI	GCTAGC	1 cut(s)	1318
NlaIII	CATG	9 cut(s)	1046, 1146, 1232, 1318, 1391, 1567, 2400, 2440, 2830
NlaIV	GGNNCC	4 cut(s)	360, 2247, 2748, 3322
NmeAIII	GCCGAG	1 cut(s)	96
NmuCI	GTSAC	3 cut(s)	56, 2392, 2735
NsbI	TGCGCA	1 cut(s)	3374
NspI	RCATGY	2 cut(s)	1046, 2400
PaeI	GCATGC	1 cut(s)	1046
PagI	TCATGA	2 cut(s)	1228, 1387
PceI	AGGCCT	1 cut(s)	1878
PciI	ACATGT	1 cut(s)	2396
PctI	GAATGC	1 cut(s)	688
PdmI	GAANNNNTTC	1 cut(s)	1983
PflMI	CCANNNNNTGG	3 cut(s)	804, 2879, 3271
PfoI	TCCNGGA	1 cut(s)	2910
PleI	GAGTC	1 cut(s)	424
PpsI	GAGTC	1 cut(s)	424
Ppu21I	YACGTR	1 cut(s)	830
PpuMI	RGGWCCY	2 cut(s)	2246, 2841
PscI	ACATGT	1 cut(s)	2396
Psp124BI	GAGCTC	1 cut(s)	1857
Psp5II	RGGWCCY	2 cut(s)	2246, 2841
PspFI	CCCAGC	1 cut(s)	2300
PspN4I	GGNNCC	4 cut(s)	360, 2247, 2748, 3322
PspPI	GGNCC	4 cut(s)	1286, 2246, 2841, 3419
PspPPI	RGGWCCY	2 cut(s)	2246, 2841
PsrI	GAACNNNNNNTAC	2 cut(s)	1779, 1811
PstI	CTGCAG	1 cut(s)	3427
PstNI	CAGNNNCTG	4 cut(s)	2300, 2720, 2917, 3422
PsuI	RGATCY	3 cut(s)	556, 1294, 1541
RsaI	GTAC	5 cut(s)	625, 828, 1119, 2113, 3168
RsaNI	GTAC	5 cut(s)	624, 827, 1118, 2112, 3167
RseI	CAYNNNNRTG	4 cut(s)	534, 1215, 1278, 2358
SacI	GAGCTC	1 cut(s)	1857
SaqAI	TTAA	5 cut(s)	974, 1248, 1961, 2760, 3473
Sau96I	GGNCC	4 cut(s)	1286, 2246, 2841, 3419
ScaI	AGTACT	1 cut(s)	1119
SchI	GAGTC	1 cut(s)	425
SduI	GDGCHC	6 cut(s)	1582, 1720, 1783, 1857, 2371, 3332
SfcI	CTRYAG	7 cut(s)	1090, 1305, 1644, 2930, 3423, 3450, 3457
SinI	GGWCC	3 cut(s)	1286, 2246, 2841
SmiMI	CAYNNNNRTG	4 cut(s)	534, 1215, 1278, 2358
SmlI	CTYRAG	3 cut(s)	1235, 1573, 3536
SmoI	CTYRAG	3 cut(s)	1235, 1573, 3536
SpeI	ACTAGT	1 cut(s)	653
SphI	GCATGC	1 cut(s)	1046
SseBI	AGGCCT	1 cut(s)	1878
SspI	AATATT	4 cut(s)	1973, 1995, 2091, 2970
SspMI	CTAG	6 cut(s)	621, 633, 654, 1298, 1319, 3132
SstI	GAGCTC	1 cut(s)	1857
StuI	AGGCCT	1 cut(s)	1878
StyI	CCWWGG	2 cut(s)	2740, 2826
TaaI	ACNGT	8 cut(s)	48, 442, 2048, 2239, 3088, 3244, 3451, 3478
TaiI	ACGT	2 cut(s)	832, 3613
TaqI	TCGA	8 cut(s)	100, 790, 1030, 1069, 1455, 1944, 2371, 3154
TatI	WGTACW	2 cut(s)	623, 1117
TauI	GCSGC	5 cut(s)	113, 180, 258, 2300, 2989
Tru1I	TTAA	5 cut(s)	974, 1248, 1961, 2760, 3473
Tru9I	TTAA	5 cut(s)	974, 1248, 1961, 2760, 3473
TscAI	CASTG	8 cut(s)	536, 986, 2053, 2479, 2512, 2526, 2784, 3403
TseFI	GTSAC	3 cut(s)	56, 2392, 2735
Tsp45I	GTSAC	3 cut(s)	56, 2392, 2735
TspDTI	ATGAA	7 cut(s)	245, 1127, 1404, 1787, 2298, 2481, 3227
TspGWI	ACGGA	6 cut(s)	44, 125, 409, 519, 2677, 3482
TspRI	CASTG	8 cut(s)	536, 986, 2053, 2479, 2512, 2526, 2784, 3403
Van91I	CCANNNNNTGG	3 cut(s)	804, 2879, 3271
VpaK11BI	GGWCC	3 cut(s)	1286, 2246, 2841
XapI	RAATTY	9 cut(s)	883, 1083, 1134, 1166, 2073, 2552, 2665, 3097, 3356
XceI	RCATGY	2 cut(s)	1046, 2400
XcmI	CCANNNNNNNNNTGG	1 cut(s)	296
XmiI	GTMKAC	2 cut(s)	906, 3453
XmnI	GAANNNNTTC	1 cut(s)	1983
XspI	CTAG	6 cut(s)	621, 633, 654, 1298, 1319, 3132
ZrmI	AGTACT	1 cut(s)	1119

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.