Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0008467

Physical Location & Seq

Genomic Coordinates Forward (+)

42174264 .. 42184211

Gene Structure 9948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG167100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGATTGCGTGCCTTCGCTGCACACCTCCGCTTCTTCTTCTTCGTCGCAGTTCCTTCGTCACAAGCAATTCAGATTCCTCCACAATCACCTTCAATGCCGTCGAAGCGTTGCGGCACTCGGCCGTACAACAAGTTTCCGTCCTCTCCATCACAAATCAAACAATCTTCGCATTCTCTGCGGCGTCGGTGTTTCTCCTCCTCAATCCACAGAGGAGCAGAAGGAGACCAAGATGAAGTCCAAGTCAAAGTCTGGGCGCGGCAAGGTTTGGCTTGATATTAGGTTGGATCACCAAGTTGCGTTTGGAGAGAGTGTTGCGGTTTTGGGATCATCAAAGGAGTTAGGGTCGTGGAAGAAGAAGGTGCCTCTGAATTGGACAGAGAGTGGATGGGTATGCAAATTGGAGTTCAAGGGTGGTGAGTCCGTTGAGTTTAAGTTTGTAACTGTTAGGGCTGACAAGAGTATGCTTTGGGAAGGTGATGATAACCGGGTTTTGAAACTTCCCAACGGAGGAAGCTTTGGGATGGTTTGCCACTGGAATGCAACTGGGGAGAATGTAGATCTATTCCCATTGGACAAGGAGGATGGTGTTGAATATAAGGGGTCTTCTGCTGCCCAAACTGCTAATACAACTGCTAGTTCTCCCGATGTGGAGACTAGTCCTTTTGTTGGTCAATGGAAAGGGAATGCCATCTCATTTATGCGGTCAAATGAGCATCGGGATCGTGAATCAGGAAGAAATTGGGACACTTCAGGTCTTGAAGGGTTGTCTCTGAAGTTGGTTGAAGGTGATCGAAATGCCAGGAATTGGTGGCAAAAGCTTGAAGTTGTACGTGATATACTACTTGAAAGTTCGCAAAGTGAGGAGCGGTTGGATGCTCTCATAAATTCTTCCATTTATTTGAAGTGGATAAACACAGGCCAGATTCCTTGCTTTGAAGGTGGAGGTCATCATCGACCAAACCGGCATGCTGAGATTTCCAGGGTTATATTTCGAGAACTGGAAAGAATTTCCTGTAGGAAAGATACTTCACCCCAGGAAGTACTTGTTATCCGCAAAATTCATCCATGTCTGCCATCGTTCAAAGCAGAATTTACTGCATCTGTCCCCCTAACACGAATCAGAGACATTGCTCATCGGAATGATATCCCTCATGACCTCAAGCAAGAAATTAAGCACACAATACAAAACAAACTTCACCGCAATGCTGGTCCTGAAGATCTAGTTGCTACAGAAGCCATGCTAGCAAGAATAACCAAGAACCCTGGACAATATAGTGAAGCATTTGTGGAGCAGTTCAAGATATTCCATCATGAGCTCAAAGACTTTTTCAATGCTGGAAGCCTTGCTGAACAACTTGAATCCATCAAAGATTCCATCGATGATAAAGGTCGTTCAGCTCTTACATTGTTTTTGGAGTGCAAAAAGGGTTTGGATGCTTCAGCAGAATCAAGTAAAGTTTTGGGGTCTGATTTGTTGTTCAAGACCATGCAATCCTTGAGTGCTCTGAGAGATATAATTGCAAAGGGTCTTGAAAGTGGCCTTAGAAATGATGCTTCTGATGCTGCTATAGCTATGCGTCAAAAGTGGCGTCTTTGCGAGATTGGACTTGAGGACTATTCATTCATCCTTCTAAGCAGATTCGCTAATGAGCTTGAAGCTATGGGAGGGGCTCATTGGCTGGCACAAGATGTGAAATCAAAGGATGTAAGCTCTTGGAATGATCCACTTGGTGCTCTTGTAGTTGGTGTTCATCAGCTAAGGTTATCTGGTTGGAAGCCTGAAGAATGTGCGGCTATTCAAAACGAGCTCCTTGCCTGGAAAGCAAGAGGTCTTTCTCAAACAGAAGCGAGTGAAGATGGCAAAACAATCTGGGGACTACGGCATAAAGCTACTCTCGATAGAGCCAGGAGGTTAACTGAAGAATATTCTGAAGCACTTCTTCAAATATTTCCCCAAAATGTCCAGGTATTAGGAAAAGCTTTTGGAATACCTGAGAACAGTGTAAGGACATACGCTGAAGCTGAAATTCGCGCTGGTGTAATATTTCAGGTTTCAAAACTCTGTTCCCTGCTTTTGAAAGCAGTTAGAACTACCATTGGTTCTCAGGGTTGGGATGTTATTGTGCCGGGAGCTGCTATGGGAACATTGGTTCAGGTTGAGAGGATTGTCCCCGGTTCAATACCATCAACTGTTGAGGGACCTGTTGTTCTCGTTGTCAACAAAGCAGATGGAGATGAAGAGGTAACAGCGGCTGGGAGTAACATTGTGGGAGTTGTTCTTCTGCAAGAGCTTCCTCACTTGTCTCATCTTGGTGTCAGGGCTCGACAAGAGAAGGTTGTGTTTGTGACATGTGAAGATGATGACAAAGTTGCTGATATACAAAAACTCATGGGAAAATATGTGAGGTTAGAAGCATCCTCGTCCAGTGTTGATATATATCCTTCAACAGAAAACAGCAATGGCACTTTTGCAGTGAAAAATGTTTCAAGTGATGTTGCACCAAAAGTTGAATCCCGTGGAACTCCTGACCCTTCCTGGTCTGCTGCTAAAACCCCAGAATCCAATAAGGGGGTATCTGCCGGTGGAGTTTTACTACTTGCTGATGCAGAAGCACGGAATTCGGGTGCAAAAGCTGCTGCTTGTGGTCGTTTAGCTTCTTTGGCAGCGGCATCTGATAAAGTTTTTAGTGACCAAGGGGTACCAGCTTCCTTTAATGTCCCTGCTGGAGCAGTTATACCATTTGGCTCTATGGAACTGGCATTAGAGCAAAGCAAATCCATGGAATCATTCAGGTCCTTTCTAAATATGATAGAAACATTGAAGCCAGAAAGTGGAGAACTTGACCAAGTATGTGGTCAGCTCCGGGAACTGATCTCTTCTCTACAGCCCTCCAAAGATATCATTGATAGAATAGCCAAAATATTTCCAGGCAATGCGCGGCTAATTGTTCGTTCAAGTGCTAATGTTGAGGACTTAGCTGGAATGTCGGCTGCTGGACTATATGATTCAATTCCCAATGTTAGTGTTTCAAATCCGACAGTATTTGCAAATTCTATAAGCAAAGTGTGGGCATCACTTTATACTAGGAGGGCAGTTTTGAGCCGTCGAGTTGCCGGTGTACCTCAGAAGGATGCTACAATGGCAATTCTGGTGCAAGAAATGCTTTCACCAGACTTATCGTTTGTGCTTCATACAGTCAGCCCAACAGACCAAGACCATAATTTGGTTGAGGCTGAAATTGCTTCTGGCCTCGGTGAAACTTTGGCTTCAGGCACCAGGGGCACACCCTGGCGTATATCGTCTGGAAAATTTGATGGGAATGTGCGCACATTGGCATTTGCTAATTTCAGTGAGGAACTACTTGGTGGAGGCCCTGCAGACGGGGAGGTTATCCATTTGACTGTAGACTACAGCAAGAAACCATTAACTGTTGATCCAGTTTTCCGTCGGCAGCTTGGTCAGCGCCTCGGTGCGGTGGGATTTTTCCTTGAGCAGAAGTTCGGCTGCCCACAGGATGTGGAAGGATGTGTTGTTGGCAAAGACATCTTTATAGTCCAGACACGTCCACAGCCCCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0019200 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046835 GO:0051752 GO:0071704 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 PPDK_N

Protein Analysis

1193

Amino Acids

130.55

Weight (kDa)

6.57

Isoelectric Point (pI)

39.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	95 - 171	2.3e-14	Starch binding domain
GWD1_pHisD	PF22973	705 - 818	2.5e-34	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	904 - 1191	1.4e-30	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26570 AT5G26570
fragaria_vesca	FvH4_4g12510 FvH4_4g12510
malus_domestica	MD04G1025900.v1.1
prunus_persica	Prupe.1G175200_v2.0.a1
pyrus_communis	pycom04g02200
rosa_chinensis	RchiOBHm_Chr4g0411251 RchiOBHm_Chr4g0411331
rosa_laevigata	RLG00000008365 RLG00000008368
rosa_multiflora	Rmu_sc0000543.1_g000027 Rmu_sc0000543.1_g000049
rosa_roxburghii	Rroxscaffold_5G00355910
rosa_rugosa	Rorug04G0110100
rosa_samantha	Rh4AG167100 Rh4BG163600 Rh4BG164200 Rh4CG178800 Rh4DG161900
rosa_wichuraiana	Rw4G013760 Rw4G013810 Rw4G013910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3329
Acc65I	GGTACC	1 cut(s)	2701
AccB1I	GGYRCC	3 cut(s)	361, 2701, 3275
AccB7I	CCANNNNNTGG	3 cut(s)	807, 2834, 3226
AccBSI	CCGCTC	1 cut(s)	868
AccI	GTMKAC	1 cut(s)	3408
AccII	CGCG	3 cut(s)	258, 2034, 2941
AclWI	GGATC	5 cut(s)	294, 334, 729, 1718, 3431
AcoI	YGGCCR	1 cut(s)	121
AcsI	RAATTY	8 cut(s)	886, 1008, 1059, 1091, 2028, 2620, 3052, 3311
AcuI	CTGAAG	9 cut(s)	735, 794, 1236, 1425, 1803, 1941, 1953, 2040, 3255
AcyI	GRCGYC	2 cut(s)	183, 1591
AdeI	CACNNNGTG	1 cut(s)	1733
AfaI	GTAC	5 cut(s)	127, 831, 1044, 2703, 3123
AfiI	CCNNNNNNNGG	8 cut(s)	509, 668, 807, 2571, 2834, 3226, 3383, 3475
AflIII	ACRYGT	2 cut(s)	2351, 3563
AhlI	ACTAGT	1 cut(s)	656
AjiI	CACGTC	1 cut(s)	3566
AloI	GAACNNNNNNTCC	4 cut(s)	2136, 2168, 2193, 2225
Alw21I	GWGCWC	4 cut(s)	1320, 1507, 1738, 1812
Alw26I	GTCTC	5 cut(s)	218, 647, 774, 1119, 2310
AlwI	GGATC	5 cut(s)	294, 334, 729, 1718, 3431
AlwNI	CAGNNNCTG	1 cut(s)	2255
AoxI	GGCC	5 cut(s)	121, 919, 1540, 3250, 3373
ApoI	RAATTY	8 cut(s)	886, 1008, 1059, 1091, 2028, 2620, 3052, 3311
ArsI	GACNNNNNNTTYG	4 cut(s)	1576, 1608, 3167, 3199
Asp700I	GAANNNNTTC	1 cut(s)	1938
Asp718I	GGTACC	1 cut(s)	2701
AspLEI	GCGC	5 cut(s)	258, 2036, 2941, 3330, 3468
AspS9I	GGNCC	4 cut(s)	1211, 2201, 2796, 3374
AsuC2I	CCSGG	4 cut(s)	488, 2130, 2175, 2867
AsuHPI	GGTGA	9 cut(s)	80, 281, 428, 488, 800, 1023, 1190, 3162, 3269
AsuNHI	GCTAGC	1 cut(s)	1243
AvaII	GGWCC	3 cut(s)	1211, 2201, 2796
BaeGI	GKGCMC	1 cut(s)	3287
BanI	GGYRCC	3 cut(s)	361, 2701, 3275
BanII	GRGCYC	4 cut(s)	1320, 1675, 1812, 2326
BbsI	GAAGAC	1 cut(s)	597
Bbv12I	GWGCWC	4 cut(s)	1320, 1507, 1738, 1812
BceAI	ACGGC	4 cut(s)	84, 108, 1898, 3090
BcgI	CGANNNNNNTGC	8 cut(s)	38, 72, 704, 738, 1796, 1830, 2604, 2638
BcnI	CCSGG	4 cut(s)	488, 2130, 2175, 2867
BcoDI	GTCTC	5 cut(s)	218, 647, 774, 1119, 2310
BcuI	ACTAGT	1 cut(s)	656
BfaI	CTAG	5 cut(s)	636, 657, 1223, 1244, 3087
BfmI	CTRYAG	7 cut(s)	1015, 1230, 1569, 2885, 3378, 3405, 3412
BfoI	RGCGCY	1 cut(s)	3469
BglII	AGATCT	2 cut(s)	559, 1219
BmcAI	AGTACT	1 cut(s)	1044
Bme18I	GGWCC	3 cut(s)	1211, 2201, 2796
BmgBI	CACGTC	1 cut(s)	3566
BmgT120I	GGNCC	4 cut(s)	1211, 2201, 2796, 3374
BmiI	GGNNCC	4 cut(s)	363, 2202, 2703, 3277
BmrI	ACTGGG	1 cut(s)	555
BmtI	GCTAGC	1 cut(s)	1247
BmuI	ACTGGG	1 cut(s)	555
BpiI	GAAGAC	1 cut(s)	597
BpmI	CTGGAG	1 cut(s)	2748
Bpu10I	CCTNAGC	1 cut(s)	1760
BpuEI	CTTGAG	4 cut(s)	1145, 1519, 1631, 3512
BpuMI	CCSGG	4 cut(s)	488, 2130, 2175, 2867
Bsa29I	ATCGAT	1 cut(s)	1380
BsaAI	YACGTR	1 cut(s)	833
BsaBI	GATNNNNATC	1 cut(s)	2439
BsaHI	GRCGYC	2 cut(s)	183, 1591
BsaI	GGTCTC	1 cut(s)	218
BsaXI	ACNNNNNCTCC	4 cut(s)	297, 327, 329, 359
Bsc4I	CCNNNNNNNGG	8 cut(s)	509, 668, 807, 2571, 2834, 3226, 3383, 3475
Bse118I	RCCGGY	3 cut(s)	963, 2582, 3116
Bse1I	ACTGG	6 cut(s)	539, 550, 1005, 2427, 2763, 3440
Bse3DI	GCAATG	4 cut(s)	1128, 1210, 2467, 2941
Bse8I	GATNNNNATC	1 cut(s)	2439
BseCI	ATCGAT	1 cut(s)	1380
BseJI	GATNNNNATC	1 cut(s)	2439
BseLI	CCNNNNNNNGG	8 cut(s)	509, 668, 807, 2571, 2834, 3226, 3383, 3475
BseMI	GCAATG	4 cut(s)	1128, 1210, 2467, 2941
BseMII	CTCAG	5 cut(s)	963, 1499, 1986, 2120, 3140
BseNI	ACTGG	6 cut(s)	539, 550, 1005, 2427, 2763, 3440
BseRI	GAGGAG	4 cut(s)	186, 189, 227, 878
BseSI	GKGCMC	1 cut(s)	3287
BseX3I	CGGCCG	1 cut(s)	121
BseYI	CCCAGC	1 cut(s)	2255
BsgI	GTGCAG	1 cut(s)	5
Bsh1236I	CGCG	3 cut(s)	258, 2034, 2941
Bsh1285I	CGRYCG	1 cut(s)	124
BshFI	GGCC	5 cut(s)	123, 921, 1542, 3252, 3375
BshNI	GGYRCC	3 cut(s)	361, 2701, 3275
BshVI	ATCGAT	1 cut(s)	1380
BsiEI	CGRYCG	1 cut(s)	124
BsiHKAI	GWGCWC	4 cut(s)	1320, 1507, 1738, 1812
BsiSI	CCGG	7 cut(s)	487, 964, 2129, 2175, 2583, 2866, 3117
BslFI	GGGAC	6 cut(s)	758, 1091, 1890, 2156, 2214, 2705
BslI	CCNNNNNNNGG	8 cut(s)	509, 668, 807, 2571, 2834, 3226, 3383, 3475
BsmAI	GTCTC	5 cut(s)	218, 647, 774, 1119, 2310
BsmFI	GGGAC	6 cut(s)	758, 1091, 1890, 2156, 2214, 2705
BsmI	GAATGC	3 cut(s)	171, 544, 691
BsnI	GGCC	5 cut(s)	123, 921, 1542, 3252, 3375
Bso31I	GGTCTC	1 cut(s)	218
Bsp1286I	GDGCHC	7 cut(s)	1320, 1507, 1675, 1738, 1812, 2326, 3287
Bsp143I	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
Bsp19I	CCATGG	1 cut(s)	2781
BspANI	GGCC	5 cut(s)	123, 921, 1542, 3252, 3375
BspCNI	CTCAG	5 cut(s)	964, 1500, 1987, 2119, 3139
BspDI	ATCGAT	1 cut(s)	1380
BspFNI	CGCG	3 cut(s)	258, 2034, 2941
BspHI	TCATGA	2 cut(s)	1153, 1312
BspLI	GGNNCC	4 cut(s)	363, 2202, 2703, 3277
BspMAI	CTGCAG	1 cut(s)	3382
BspOI	GCTAGC	1 cut(s)	1247
BspPI	GGATC	5 cut(s)	294, 334, 729, 1718, 3431
BspT107I	GGYRCC	3 cut(s)	361, 2701, 3275
BspTNI	GGTCTC	1 cut(s)	218
BsrBI	CCGCTC	1 cut(s)	868
BsrDI	GCAATG	4 cut(s)	1128, 1210, 2467, 2941
BsrFI	RCCGGY	3 cut(s)	963, 2582, 3116
BsrI	ACTGG	6 cut(s)	539, 550, 1005, 2427, 2763, 3440
BssAI	RCCGGY	3 cut(s)	963, 2582, 3116
BssMI	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
BssNI	GRCGYC	2 cut(s)	183, 1591
BssT1I	CCWWGG	2 cut(s)	2695, 2781
Bst4CI	ACNGT	7 cut(s)	445, 2003, 2194, 3043, 3199, 3406, 3433
Bst6I	CTCTTC	2 cut(s)	2235, 2884
BstACI	GRCGYC	2 cut(s)	183, 1591
BstAPI	GCANNNNNTGC	3 cut(s)	177, 2636, 3163
BstBAI	YACGTR	1 cut(s)	833
BstC8I	GCNNGC	4 cut(s)	11, 969, 1245, 1683
BstDEI	CTNAG	9 cut(s)	972, 1508, 1544, 1634, 1760, 1995, 2106, 2977, 3126
BstDSI	CCRYGG	2 cut(s)	2518, 2781
BstFNI	CGCG	3 cut(s)	258, 2034, 2941
BstH2I	RGCGCY	1 cut(s)	3469
BstHHI	GCGC	5 cut(s)	258, 2036, 2941, 3330, 3468
BstKTI	GATC	9 cut(s)	289, 329, 562, 724, 793, 1222, 1726, 2877, 3439
BstMAI	GTCTC	5 cut(s)	218, 647, 774, 1119, 2310
BstMBI	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
BstMCI	CGRYCG	1 cut(s)	124
BstNSI	RCATGY	2 cut(s)	971, 2355
BstSFI	CTRYAG	7 cut(s)	1015, 1230, 1569, 2885, 3378, 3405, 3412
BstSLI	GKGCMC	1 cut(s)	3287
BstUI	CGCG	3 cut(s)	258, 2034, 2941
BstV2I	GAAGAC	1 cut(s)	597
BstX2I	RGATCY	2 cut(s)	559, 1219
BstXI	CCANNNNNNTGG	1 cut(s)	2855
BstYI	RGATCY	2 cut(s)	559, 1219
BstZI	CGGCCG	1 cut(s)	121
Bsu15I	ATCGAT	1 cut(s)	1380
BsuRI	GGCC	5 cut(s)	123, 921, 1542, 3252, 3375
BsuTUI	ATCGAT	1 cut(s)	1380
BtgI	CCRYGG	2 cut(s)	2518, 2781
BtrI	CACGTC	1 cut(s)	3566
BtsI	GCAGTG	1 cut(s)	2481
BtsIMutI	CAGTG	5 cut(s)	532, 2008, 2434, 2481, 3358
Cac8I	GCNNGC	4 cut(s)	11, 969, 1245, 1683
CaiI	CAGNNNCTG	1 cut(s)	2255
CciI	TCATGA	2 cut(s)	1153, 1312
CfoI	GCGC	5 cut(s)	258, 2036, 2941, 3330, 3468
Cfr10I	RCCGGY	3 cut(s)	963, 2582, 3116
Cfr13I	GGNCC	4 cut(s)	1211, 2201, 2796, 3374
ClaI	ATCGAT	1 cut(s)	1380
CseI	GACGC	3 cut(s)	172, 1568, 1580
Csp6I	GTAC	5 cut(s)	126, 830, 1043, 2702, 3122
CviAII	CATG	9 cut(s)	968, 1068, 1154, 1240, 1313, 1489, 2352, 2392, 2782
CviQI	GTAC	5 cut(s)	126, 830, 1043, 2702, 3122
DdeI	CTNAG	9 cut(s)	972, 1508, 1544, 1634, 1760, 1995, 2106, 2977, 3126
DpnI	GATC	9 cut(s)	288, 328, 561, 723, 792, 1221, 1725, 2876, 3438
DpnII	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
DraIII	CACNNNGTG	1 cut(s)	1733
EaeI	YGGCCR	1 cut(s)	121
EagI	CGGCCG	1 cut(s)	121
Eam1104I	CTCTTC	2 cut(s)	2235, 2884
EarI	CTCTTC	2 cut(s)	2235, 2884
Ecl136II	GAGCTC	2 cut(s)	1318, 1810
EclXI	CGGCCG	1 cut(s)	121
Eco130I	CCWWGG	2 cut(s)	2695, 2781
Eco24I	GRGCYC	4 cut(s)	1320, 1675, 1812, 2326
Eco31I	GGTCTC	1 cut(s)	218
Eco32I	GATATC	2 cut(s)	1147, 2902
Eco47I	GGWCC	3 cut(s)	1211, 2201, 2796
Eco52I	CGGCCG	1 cut(s)	121
Eco53kI	GAGCTC	2 cut(s)	1318, 1810
Eco57I	CTGAAG	9 cut(s)	735, 794, 1236, 1425, 1803, 1941, 1953, 2040, 3255
EcoICRI	GAGCTC	2 cut(s)	1318, 1810
EcoO109I	RGGNCCY	3 cut(s)	2201, 2796, 3374
EcoRI	GAATTC	1 cut(s)	2620
EcoRV	GATATC	2 cut(s)	1147, 2902
EcoT14I	CCWWGG	2 cut(s)	2695, 2781
EcoT38I	GRGCYC	4 cut(s)	1320, 1675, 1812, 2326
ErhI	CCWWGG	2 cut(s)	2695, 2781
FaeI	CATG	9 cut(s)	971, 1071, 1157, 1243, 1316, 1492, 2355, 2395, 2785
FalI	AAGNNNNNCTT	4 cut(s)	1926, 1958, 3150, 3182
FaqI	GGGAC	6 cut(s)	758, 1091, 1890, 2156, 2214, 2705
FatI	CATG	9 cut(s)	967, 1067, 1153, 1239, 1312, 1488, 2351, 2391, 2781
FblI	GTMKAC	1 cut(s)	3408
FriOI	GRGCYC	4 cut(s)	1320, 1675, 1812, 2326
FspAI	RTGCGCAY	1 cut(s)	3329
FspBI	CTAG	5 cut(s)	636, 657, 1223, 1244, 3087
FspI	TGCGCA	1 cut(s)	3329
GlaI	GCGC	5 cut(s)	257, 2035, 2940, 3329, 3467
GsaI	CCCAGC	1 cut(s)	2259
GsuI	CTGGAG	1 cut(s)	2748
HaeII	RGCGCY	1 cut(s)	3469
HaeIII	GGCC	5 cut(s)	123, 921, 1542, 3252, 3375
HapII	CCGG	7 cut(s)	487, 964, 2129, 2175, 2583, 2866, 3117
HgaI	GACGC	3 cut(s)	172, 1568, 1580
HhaI	GCGC	5 cut(s)	258, 2036, 2941, 3330, 3468
Hin1I	GRCGYC	2 cut(s)	183, 1591
Hin1II	CATG	9 cut(s)	971, 1071, 1157, 1243, 1316, 1492, 2355, 2395, 2785
Hin6I	GCGC	5 cut(s)	256, 2034, 2939, 3328, 3466
HinP1I	GCGC	5 cut(s)	256, 2034, 2939, 3328, 3466
HincII	GTYRAC	2 cut(s)	1917, 2221
HindII	GTYRAC	2 cut(s)	1917, 2221
HindIII	AAGCTT	3 cut(s)	514, 818, 1980
HpaI	GTTAAC	1 cut(s)	1917
HpaII	CCGG	7 cut(s)	487, 964, 2129, 2175, 2583, 2866, 3117
HphI	GGTGA	9 cut(s)	80, 281, 428, 488, 800, 1023, 1190, 3162, 3269
Hpy166II	GTNNAC	5 cut(s)	1917, 2221, 3122, 3409, 3569
Hpy8I	GTNNAC	5 cut(s)	1917, 2221, 3122, 3409, 3569
Hpy99I	CGWCG	5 cut(s)	49, 105, 188, 3111, 3453
HpyCH4III	ACNGT	7 cut(s)	445, 2003, 2194, 3043, 3199, 3406, 3433
HpyCH4IV	ACGT	2 cut(s)	832, 3565
HpyF3I	CTNAG	9 cut(s)	972, 1508, 1544, 1634, 1760, 1995, 2106, 2977, 3126
HpySE526I	ACGT	2 cut(s)	832, 3565
Hsp92I	GRCGYC	2 cut(s)	183, 1591
Hsp92II	CATG	9 cut(s)	971, 1071, 1157, 1243, 1316, 1492, 2355, 2395, 2785
HspAI	GCGC	5 cut(s)	256, 2034, 2939, 3328, 3466
KpnI	GGTACC	1 cut(s)	2705
KspAI	GTTAAC	1 cut(s)	1917
Kzo9I	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
LmnI	GCTCC	7 cut(s)	214, 865, 1291, 1815, 2132, 2729, 2868
MaeI	CTAG	5 cut(s)	636, 657, 1223, 1244, 3087
MaeII	ACGT	2 cut(s)	832, 3565
MaeIII	GTNAC	6 cut(s)	59, 439, 2245, 2261, 2347, 2690
MalI	GATC	9 cut(s)	288, 328, 561, 723, 792, 1221, 1725, 2876, 3438
MbiI	CCGCTC	1 cut(s)	868
MboI	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
MflI	RGATCY	2 cut(s)	559, 1219
MhlI	GDGCHC	7 cut(s)	1320, 1507, 1675, 1738, 1812, 2326, 3287
MlyI	GAGTC	1 cut(s)	428
MmeI	TCCRAC	4 cut(s)	264, 852, 1754, 3062
MroXI	GAANNNNTTC	1 cut(s)	1938
MseI	TTAA	5 cut(s)	432, 1173, 1916, 2715, 3428
MslI	CAYNNNNRTG	4 cut(s)	537, 1140, 1203, 2313
MspA1I	CMGCKG	2 cut(s)	2252, 2669
MspI	CCGG	7 cut(s)	487, 964, 2129, 2175, 2583, 2866, 3117
Mva1269I	GAATGC	3 cut(s)	171, 544, 691
MvnI	CGCG	3 cut(s)	258, 2034, 2941
NciI	CCSGG	4 cut(s)	488, 2130, 2175, 2867
NcoI	CCATGG	1 cut(s)	2781
NdeII	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
NheI	GCTAGC	1 cut(s)	1243
NlaIII	CATG	9 cut(s)	971, 1071, 1157, 1243, 1316, 1492, 2355, 2395, 2785
NlaIV	GGNNCC	4 cut(s)	363, 2202, 2703, 3277
NmeAIII	GCCGAG	1 cut(s)	99
NmuCI	GTSAC	3 cut(s)	59, 2347, 2690
NsbI	TGCGCA	1 cut(s)	3329
NspI	RCATGY	2 cut(s)	971, 2355
PaeI	GCATGC	1 cut(s)	971
PagI	TCATGA	2 cut(s)	1153, 1312
PciI	ACATGT	1 cut(s)	2351
PctI	GAATGC	3 cut(s)	171, 544, 691
PdmI	GAANNNNTTC	1 cut(s)	1938
PflMI	CCANNNNNTGG	3 cut(s)	807, 2834, 3226
PfoI	TCCNGGA	1 cut(s)	2865
PleI	GAGTC	1 cut(s)	427
PpsI	GAGTC	1 cut(s)	427
Ppu21I	YACGTR	1 cut(s)	833
PpuMI	RGGWCCY	2 cut(s)	2201, 2796
PscI	ACATGT	1 cut(s)	2351
Psp124BI	GAGCTC	2 cut(s)	1320, 1812
Psp5II	RGGWCCY	2 cut(s)	2201, 2796
PspFI	CCCAGC	1 cut(s)	2255
PspN4I	GGNNCC	4 cut(s)	363, 2202, 2703, 3277
PspPI	GGNCC	4 cut(s)	1211, 2201, 2796, 3374
PspPPI	RGGWCCY	2 cut(s)	2201, 2796
PsrI	GAACNNNNNNTAC	2 cut(s)	1734, 1766
PstI	CTGCAG	1 cut(s)	3382
PstNI	CAGNNNCTG	1 cut(s)	2255
PsuI	RGATCY	2 cut(s)	559, 1219
RsaI	GTAC	5 cut(s)	127, 831, 1044, 2703, 3123
RsaNI	GTAC	5 cut(s)	126, 830, 1043, 2702, 3122
RseI	CAYNNNNRTG	4 cut(s)	537, 1140, 1203, 2313
SacI	GAGCTC	2 cut(s)	1320, 1812
SaqAI	TTAA	5 cut(s)	432, 1173, 1916, 2715, 3428
Sau3AI	GATC	9 cut(s)	286, 326, 559, 721, 790, 1219, 1723, 2874, 3436
Sau96I	GGNCC	4 cut(s)	1211, 2201, 2796, 3374
ScaI	AGTACT	1 cut(s)	1044
SchI	GAGTC	1 cut(s)	428
SduI	GDGCHC	7 cut(s)	1320, 1507, 1675, 1738, 1812, 2326, 3287
SfcI	CTRYAG	7 cut(s)	1015, 1230, 1569, 2885, 3378, 3405, 3412
SinI	GGWCC	3 cut(s)	1211, 2201, 2796
SmiMI	CAYNNNNRTG	4 cut(s)	537, 1140, 1203, 2313
SmlI	CTYRAG	4 cut(s)	1160, 1498, 1610, 3491
SmoI	CTYRAG	4 cut(s)	1160, 1498, 1610, 3491
SpeI	ACTAGT	1 cut(s)	656
SphI	GCATGC	1 cut(s)	971
SspI	AATATT	4 cut(s)	1928, 1950, 2046, 2925
SspMI	CTAG	5 cut(s)	636, 657, 1223, 1244, 3087
SstI	GAGCTC	2 cut(s)	1320, 1812
StyI	CCWWGG	2 cut(s)	2695, 2781
TaaI	ACNGT	7 cut(s)	445, 2003, 2194, 3043, 3199, 3406, 3433
TaiI	ACGT	2 cut(s)	835, 3568
TaqI	TCGA	8 cut(s)	103, 793, 955, 994, 1380, 1899, 2326, 3109
TatI	WGTACW	1 cut(s)	1042
TauI	GCSGC	7 cut(s)	116, 183, 261, 1796, 2255, 2672, 2944
Tru1I	TTAA	5 cut(s)	432, 1173, 1916, 2715, 3428
Tru9I	TTAA	5 cut(s)	432, 1173, 1916, 2715, 3428
TscAI	CASTG	5 cut(s)	539, 2008, 2434, 2481, 3358
TseFI	GTSAC	3 cut(s)	59, 2347, 2690
Tsp45I	GTSAC	3 cut(s)	59, 2347, 2690
TspDTI	ATGAA	7 cut(s)	248, 1052, 1611, 1615, 1742, 2253, 3182
TspGWI	ACGGA	5 cut(s)	128, 412, 522, 2632, 3437
TspRI	CASTG	5 cut(s)	539, 2008, 2434, 2481, 3358
Van91I	CCANNNNNTGG	3 cut(s)	807, 2834, 3226
VpaK11BI	GGWCC	3 cut(s)	1211, 2201, 2796
XapI	RAATTY	8 cut(s)	886, 1008, 1059, 1091, 2028, 2620, 3052, 3311
XceI	RCATGY	2 cut(s)	971, 2355
XcmI	CCANNNNNNNNNTGG	1 cut(s)	299
XmiI	GTMKAC	1 cut(s)	3408
XmnI	GAANNNNTTC	1 cut(s)	1938
XspI	CTAG	5 cut(s)	636, 657, 1223, 1244, 3087
ZrmI	AGTACT	1 cut(s)	1044

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.