Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0008467

Physical Location & Seq

Genomic Coordinates Forward (+)

32229323 .. 32239283

Gene Structure 9961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG161900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3591 bp

ATGGATTGCGTGCCTTCGCTGCACACCTCCGCTTCTTCTTCTTCTTCTTCGTCGCAGTTCCTTCGTCACAAGCAATTCAGATTCCTCCACAATCACCTTCAATGCCGTCGAAGCGTTGCGGCACTCGGCCGTAGTAGAACAAGTTTCCGTCCTCTCCATCACAAATCAAACAATCTTCGCATTCTCTGCGGCGTCGGTGTTTCTCCTCCTCAATCCACAGAGGAGCAGAAGGAGACCAAGATGAAGTCCAAGTCAAAGTCTGGGCGCGGCAAGGTTTGGCTTGATATTAGGTTGGATCACCAAGTTGCGTTTGGAGAGAGTGTTGCGGTTTTGGGATCATCAAAGGAGTTAGGGTCGTGGAAGAAGAAGGTGCCTCTGAATTGGACAGAGAGTGGATGGGTATGCAAATTGGAGTTCAAGGGGGCTGAGTCCATTGAGTATAAGTTTGTAACTGTTGGGGCTGACAAGAGTATGCTTTGGGAAGGTGGTGATAACCGGGTTTTGAAACTTCCCAACGGAGGAAGCTTTGGGATGGTTTGCCGCTGGAATGCAACTGGGGAGAATGTAGATCTATTCCCATTGGACAATGAGGATGGTGTTGAATATAAGGGGTCTTCTGCTGCCCAAACTGCTAATACAACTGCCAGTTCTCCAGATGTGGAGACTAGTCCTTTTGTTGGTCAATGGAAAGGGAATGCCATCTCATTTATGCGGTCAAATGAGCATCGGGATCGTGAATCAGGAAGAAATTGGGACACTTCAGGTCTTGAAGGGTTGTCTCTTAAGTTGGTTGAAGGTGATCGAAATGCCAGGAATTGGTGGCAAAAGCTTGAAGTTGTACGAGATATACTACTTGAAAGTTCGCAAAGTGAGGAGCGGTTGGATGCTCTCATAAATTCTTCCATTTATTTGAAGTGGATAAACACAGGCCAGATTCCTTGCTTTGAAGGTGGAGGTCATCATCGACCAAACCGGCATGCTGAGATTTCCAGGGTTATATTTCGAGAACTGGAAAGAATTTCCTGTAGGAAAGATACTTCACCCCAGGAAGTACTTGTTATCCGCAAAATTCATCCATGTCTGCCATCGTTCAAAGCAGAATTTACTGCATCTGTCCCCCTAACACGAATCAGAGACATTGCTCATCGGAATGATATCCCTCATGACCTCAAGCAAGAAATTAAGCACACAATACAAAACAAACTTCACCGCAATGCTGGTCCTGAAGATCTAGTTGCTACAGAAGCCATGCTAGCAAGAATAACCAAGAACCCTGGACAATATAGTGAAGCATTTGTGGAGCAGTTCAAGATATTCCATCATGAGCTCAAAGACTTTTTCAATGCTGGAAGCCTTGCTGAACAACTTGAATCCATCAAAGATTCCATCGATGATAAAGGCCGTTCAGCTCTTACATTGTTTTTGGAGTGCAAAAAGGGTTTGGATGCTTCAGCAGAATCAAGTAAAGTTTTGGGGTCTGATTTGTTGTTCAAGACCATGCAATCCTTGAGTGCTCTGAGAGATATAATTGCAAAGGGTCTTGAAAGTGGCCTTAGAAATGATGCTTCTGATGCTGCTATAGCTATGCGTCAAAAGTGGCGTCTTTGCGAGATTGGACTTGAGGACTATTCATTCATCCTTCTAAGCAGATTCGCTAATGAGCTTGAAGCTATGGGAGGGGCTCATTGGCTGGCACAAGATGTGAAATCAAAGGATGTAAGCTCTTGGAATGATCCACTTGGTGCTCTTGTAGTTGGTGTTCATCAGCTAAGGTTATCTGGTTGGAAGCCTGAAGAATGTGTGGCTATTCAAAATGAGCTCCTTGCCTGGAAAGCAAGAGGTCTTTCTCAAACAGAAGCGAGTGAAGATGGCAAAACAATCTGGGGACTACGACATAAAGCTACTCTCGATAGAGCCAGGAGGTTAACTGAAGAATATTCTGAAGCACTTCTTCAAATATTTCCCCAAAATGTCCAGGTATTAGGAAAAGCTTTTGGAATACCTGAGAACAGTGTAAGGACATACGCTGAAGCTGAAATTCGCGCTGGTGTAATATTTCAGGTTTCAAAACTCTGTACCCTGCTTTTGAAAGCAGTTAGAACTACCATTGGTTCTCAGGGTTGGGATGTTATTGTGCCGGGAGCTGCTATGGGAACATTGGTTCAGGTTGAGAGGATTGTCCCCGGTTCAATACCATCAACTGTTGAGGGACCTGTTGTTCTCGTTGTCAACAAAGCAGATGGAGATGAAGAGGTAACAGCGGCTGGGAGTAACATTGTGGGAGTTGTTCTTCTGCAAGAGCTTCCTCACTTGTCTCATCTTGGTGTCAGGGCTCGACAAGAGAAGGTTGTGTTTGTGACATGTGAAGATGATGACAAAGTTGCTGATATACAAAAACTCATGGGAAAATATGTGAGGTTAGAAGCATCCTCGTCCAGTGTTGATATATATCCTTCAACAGAAAACAGCAATGGCACTTTTGCAGTGAAAAATGTTTCAAGTGATGTTGCACCAAAAGTTGAATCCCGTGGAACTCCTGACCCTTCCTGGTCTGCTGCTAAAACCCCAGAATCCAATAAGGGGGTATCTGCCGGTGGAGTTTTACTACTTGCTGATGCAGAAGCACGGAATTCGGGTGCAAAAGCTGCTGCTTGTGGTCGTTTAGCTTCTTTGGCAGCGGCATCTGATAAAGTTTTTAGTGACCAAGGGGTACCAGCTTCCTTTAATGTCCCTGCTGGAGCAGTGATACCATTTGGCTCTATGGAACTGGCATTAGAGCAAAGCAAATCCATGGAATCATTCAGGTCCTTTCTAAATATGATAGAAACATTGAAGCCAGAAAGTGGAGAACTTGACCAAGTATGTGGTCAGCTCCAGGAACTGATCTCTTCTCTACAGCCCTCCAAAGATATCATTGATAGAATAGCCAAAATATTTCCAGGCAATGCGCGGCTAATTGTTCGTTCAAGTGCTAATGTTGAGGACTTAGCTGGAATGTCGGCTGCTGGACTATATGATTCAATTCCCAATGTTAGTGTTTCAAATCCGACAGTATTTGCAAATTCTATAAGCAAAGTGTGGGCATCACTTTATACTAGGAGGGCAGTTTTGAGCCGTCGAGTTGCCGGTGTACCTCAGAAGGATGCTACAATGGCAATTCTGGTGCAAGAAATGCTTTCACCAGACTTATCGTTTGTGCTTCATACAGTCAGCCCAACAGACCAAGACCATAATTTGGTTGAGGCTGAAATTGCTTCTGGCCTCGGTGAAACTTTGGCTTCAGGCACCAGGGGCACACCCTGGCGTATATCATCTGGAAAATTTGATGGGAATGTGCGCACATTGGCATTTGCTAATTTCAGTGAGGAACTACTTGGTGGAGGCCCTGCAGACGGGGAGGTTATCCATTTGACTGTAGACTACAGCAAGAAACCATTAACTGTTGATCCAGTTTTCCGTCGGCAGCTTGGTCAGCGCCTCGGTGCGGTGGGATTTTTCCTTGAGCAGAAGTTCGGCTGCCCACAGGATGTGGAAGGATGTGTTGTTGGCAAAGACATCTTTATAGTCCAGACACGTCCACAGCCCCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0019200 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046835 GO:0051752 GO:0071704 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM_20 PPDK_N

Protein Analysis

1196

Amino Acids

130.77

Weight (kDa)

6.55

Isoelectric Point (pI)

41.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_20	PF00686	98 - 174	2.5e-14	Starch binding domain
GWD1_pHisD	PF22973	708 - 821	2.5e-34	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	907 - 1194	1.9e-30	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26570 AT5G26570
fragaria_vesca	FvH4_4g12510 FvH4_4g12510
malus_domestica	MD04G1025900.v1.1
prunus_persica	Prupe.1G175200_v2.0.a1
pyrus_communis	pycom04g02200
rosa_chinensis	RchiOBHm_Chr4g0411251 RchiOBHm_Chr4g0411331
rosa_laevigata	RLG00000008365 RLG00000008368
rosa_multiflora	Rmu_sc0000543.1_g000027 Rmu_sc0000543.1_g000049
rosa_roxburghii	Rroxscaffold_5G00355910
rosa_rugosa	Rorug04G0110100
rosa_samantha	Rh4AG167100 Rh4BG163600 Rh4BG164200 Rh4CG178800 Rh4DG161900
rosa_wichuraiana	Rw4G013760 Rw4G013810 Rw4G013910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3338
Acc65I	GGTACC	1 cut(s)	2710
AccB1I	GGYRCC	3 cut(s)	370, 2710, 3284
AccB7I	CCANNNNNTGG	3 cut(s)	816, 2843, 3235
AccBSI	CCGCTC	1 cut(s)	877
AccI	GTMKAC	1 cut(s)	3417
AccII	CGCG	3 cut(s)	267, 2043, 2950
AclWI	GGATC	5 cut(s)	303, 343, 738, 1727, 3440
AcoI	YGGCCR	1 cut(s)	127
AcsI	RAATTY	8 cut(s)	895, 1017, 1068, 1100, 2037, 2629, 3061, 3320
AcuI	CTGAAG	8 cut(s)	744, 1245, 1434, 1812, 1950, 1962, 2049, 3264
AcyI	GRCGYC	2 cut(s)	192, 1600
AdeI	CACNNNGTG	1 cut(s)	1742
AfaI	GTAC	5 cut(s)	840, 1053, 2077, 2712, 3132
AfiI	CCNNNNNNNGG	8 cut(s)	518, 677, 816, 2580, 2843, 3235, 3392, 3484
AflII	CTTAAG	1 cut(s)	782
AflIII	ACRYGT	2 cut(s)	2360, 3572
AhlI	ACTAGT	1 cut(s)	665
AjiI	CACGTC	1 cut(s)	3575
AloI	GAACNNNNNNTCC	4 cut(s)	2145, 2177, 2202, 2234
Alw21I	GWGCWC	4 cut(s)	1329, 1516, 1747, 1821
Alw26I	GTCTC	5 cut(s)	227, 656, 783, 1128, 2319
AlwI	GGATC	5 cut(s)	303, 343, 738, 1727, 3440
AlwNI	CAGNNNCTG	2 cut(s)	2264, 2881
AoxI	GGCC	6 cut(s)	127, 928, 1399, 1549, 3259, 3382
ApoI	RAATTY	8 cut(s)	895, 1017, 1068, 1100, 2037, 2629, 3061, 3320
ArsI	GACNNNNNNTTYG	4 cut(s)	1585, 1617, 3176, 3208
Asp700I	GAANNNNTTC	1 cut(s)	1947
Asp718I	GGTACC	1 cut(s)	2710
AspLEI	GCGC	5 cut(s)	267, 2045, 2950, 3339, 3477
AspS9I	GGNCC	4 cut(s)	1220, 2210, 2805, 3383
AsuC2I	CCSGG	3 cut(s)	497, 2139, 2184
AsuHPI	GGTGA	8 cut(s)	86, 290, 500, 809, 1032, 1199, 3171, 3278
AsuNHI	GCTAGC	1 cut(s)	1252
AvaII	GGWCC	3 cut(s)	1220, 2210, 2805
BaeGI	GKGCMC	1 cut(s)	3296
BanI	GGYRCC	3 cut(s)	370, 2710, 3284
BanII	GRGCYC	4 cut(s)	1329, 1684, 1821, 2335
BbsI	GAAGAC	1 cut(s)	606
Bbv12I	GWGCWC	4 cut(s)	1329, 1516, 1747, 1821
BceAI	ACGGC	4 cut(s)	90, 114, 1386, 3099
BcgI	CGANNNNNNTGC	6 cut(s)	44, 78, 713, 747, 2613, 2647
BcnI	CCSGG	3 cut(s)	497, 2139, 2184
BcoDI	GTCTC	5 cut(s)	227, 656, 783, 1128, 2319
BcuI	ACTAGT	1 cut(s)	665
BfaI	CTAG	4 cut(s)	666, 1232, 1253, 3096
BfmI	CTRYAG	7 cut(s)	1024, 1239, 1578, 2894, 3387, 3414, 3421
BfoI	RGCGCY	1 cut(s)	3478
BfrI	CTTAAG	1 cut(s)	782
BglII	AGATCT	2 cut(s)	568, 1228
BmcAI	AGTACT	1 cut(s)	1053
Bme18I	GGWCC	3 cut(s)	1220, 2210, 2805
BmgBI	CACGTC	1 cut(s)	3575
BmgT120I	GGNCC	4 cut(s)	1220, 2210, 2805, 3383
BmiI	GGNNCC	4 cut(s)	372, 2211, 2712, 3286
BmrI	ACTGGG	1 cut(s)	564
BmtI	GCTAGC	1 cut(s)	1256
BmuI	ACTGGG	1 cut(s)	564
BpiI	GAAGAC	1 cut(s)	606
BpmI	CTGGAG	3 cut(s)	636, 2757, 2858
Bpu10I	CCTNAGC	1 cut(s)	1769
BpuEI	CTTGAG	4 cut(s)	1154, 1528, 1640, 3521
BpuMI	CCSGG	3 cut(s)	497, 2139, 2184
Bsa29I	ATCGAT	1 cut(s)	1389
BsaBI	GATNNNNATC	1 cut(s)	2448
BsaHI	GRCGYC	2 cut(s)	192, 1600
BsaI	GGTCTC	1 cut(s)	227
BsaXI	ACNNNNNCTCC	4 cut(s)	306, 336, 338, 368
Bsc4I	CCNNNNNNNGG	8 cut(s)	518, 677, 816, 2580, 2843, 3235, 3392, 3484
Bse118I	RCCGGY	3 cut(s)	972, 2591, 3125
Bse1I	ACTGG	6 cut(s)	559, 645, 1014, 2436, 2772, 3449
Bse3DI	GCAATG	4 cut(s)	1137, 1219, 2476, 2950
Bse8I	GATNNNNATC	1 cut(s)	2448
BseCI	ATCGAT	1 cut(s)	1389
BseJI	GATNNNNATC	1 cut(s)	2448
BseLI	CCNNNNNNNGG	8 cut(s)	518, 677, 816, 2580, 2843, 3235, 3392, 3484
BseMI	GCAATG	4 cut(s)	1137, 1219, 2476, 2950
BseMII	CTCAG	6 cut(s)	417, 972, 1508, 1995, 2129, 3149
BseNI	ACTGG	6 cut(s)	559, 645, 1014, 2436, 2772, 3449
BseRI	GAGGAG	4 cut(s)	195, 198, 236, 887
BseSI	GKGCMC	1 cut(s)	3296
BseX3I	CGGCCG	1 cut(s)	127
BseYI	CCCAGC	1 cut(s)	2264
BsgI	GTGCAG	1 cut(s)	5
Bsh1236I	CGCG	3 cut(s)	267, 2043, 2950
Bsh1285I	CGRYCG	1 cut(s)	130
BshFI	GGCC	6 cut(s)	129, 930, 1401, 1551, 3261, 3384
BshNI	GGYRCC	3 cut(s)	370, 2710, 3284
BshVI	ATCGAT	1 cut(s)	1389
BsiEI	CGRYCG	1 cut(s)	130
BsiHKAI	GWGCWC	4 cut(s)	1329, 1516, 1747, 1821
BsiSI	CCGG	6 cut(s)	496, 973, 2138, 2184, 2592, 3126
BslFI	GGGAC	6 cut(s)	767, 1100, 1899, 2165, 2223, 2714
BslI	CCNNNNNNNGG	8 cut(s)	518, 677, 816, 2580, 2843, 3235, 3392, 3484
BsmAI	GTCTC	5 cut(s)	227, 656, 783, 1128, 2319
BsmFI	GGGAC	6 cut(s)	767, 1100, 1899, 2165, 2223, 2714
BsmI	GAATGC	3 cut(s)	180, 553, 700
BsnI	GGCC	6 cut(s)	129, 930, 1401, 1551, 3261, 3384
Bso31I	GGTCTC	1 cut(s)	227
Bsp1286I	GDGCHC	7 cut(s)	1329, 1516, 1684, 1747, 1821, 2335, 3296
Bsp143I	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
Bsp19I	CCATGG	1 cut(s)	2790
BspANI	GGCC	6 cut(s)	129, 930, 1401, 1551, 3261, 3384
BspCNI	CTCAG	6 cut(s)	418, 973, 1509, 1996, 2128, 3148
BspDI	ATCGAT	1 cut(s)	1389
BspFNI	CGCG	3 cut(s)	267, 2043, 2950
BspHI	TCATGA	2 cut(s)	1162, 1321
BspLI	GGNNCC	4 cut(s)	372, 2211, 2712, 3286
BspMAI	CTGCAG	1 cut(s)	3391
BspOI	GCTAGC	1 cut(s)	1256
BspPI	GGATC	5 cut(s)	303, 343, 738, 1727, 3440
BspT107I	GGYRCC	3 cut(s)	370, 2710, 3284
BspTI	CTTAAG	1 cut(s)	782
BspTNI	GGTCTC	1 cut(s)	227
BsrBI	CCGCTC	1 cut(s)	877
BsrDI	GCAATG	4 cut(s)	1137, 1219, 2476, 2950
BsrFI	RCCGGY	3 cut(s)	972, 2591, 3125
BsrI	ACTGG	6 cut(s)	559, 645, 1014, 2436, 2772, 3449
BssAI	RCCGGY	3 cut(s)	972, 2591, 3125
BssMI	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
BssNI	GRCGYC	2 cut(s)	192, 1600
BssT1I	CCWWGG	2 cut(s)	2704, 2790
Bst4CI	ACNGT	7 cut(s)	454, 2012, 2203, 3052, 3208, 3415, 3442
Bst6I	CTCTTC	2 cut(s)	2244, 2893
BstACI	GRCGYC	2 cut(s)	192, 1600
BstAFI	CTTAAG	1 cut(s)	782
BstAPI	GCANNNNNTGC	3 cut(s)	186, 2645, 3172
BstC8I	GCNNGC	4 cut(s)	11, 978, 1254, 1692
BstDSI	CCRYGG	2 cut(s)	2527, 2790
BstFNI	CGCG	3 cut(s)	267, 2043, 2950
BstH2I	RGCGCY	1 cut(s)	3478
BstHHI	GCGC	5 cut(s)	267, 2045, 2950, 3339, 3477
BstKTI	GATC	9 cut(s)	298, 338, 571, 733, 802, 1231, 1735, 2886, 3448
BstMAI	GTCTC	5 cut(s)	227, 656, 783, 1128, 2319
BstMBI	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
BstMCI	CGRYCG	1 cut(s)	130
BstNSI	RCATGY	2 cut(s)	980, 2364
BstSFI	CTRYAG	7 cut(s)	1024, 1239, 1578, 2894, 3387, 3414, 3421
BstSLI	GKGCMC	1 cut(s)	3296
BstUI	CGCG	3 cut(s)	267, 2043, 2950
BstV2I	GAAGAC	1 cut(s)	606
BstX2I	RGATCY	2 cut(s)	568, 1228
BstXI	CCANNNNNNTGG	1 cut(s)	2864
BstYI	RGATCY	2 cut(s)	568, 1228
BstZI	CGGCCG	1 cut(s)	127
Bsu15I	ATCGAT	1 cut(s)	1389
BsuRI	GGCC	6 cut(s)	129, 930, 1401, 1551, 3261, 3384
BsuTUI	ATCGAT	1 cut(s)	1389
BtgI	CCRYGG	2 cut(s)	2527, 2790
BtrI	CACGTC	1 cut(s)	3575
BtsI	GCAGTG	2 cut(s)	2490, 2748
BtsIMutI	CAGTG	5 cut(s)	2017, 2443, 2490, 2748, 3367
Cac8I	GCNNGC	4 cut(s)	11, 978, 1254, 1692
CaiI	CAGNNNCTG	2 cut(s)	2264, 2881
CciI	TCATGA	2 cut(s)	1162, 1321
CfoI	GCGC	5 cut(s)	267, 2045, 2950, 3339, 3477
Cfr10I	RCCGGY	3 cut(s)	972, 2591, 3125
Cfr13I	GGNCC	4 cut(s)	1220, 2210, 2805, 3383
ClaI	ATCGAT	1 cut(s)	1389
CseI	GACGC	3 cut(s)	181, 1577, 1589
Csp6I	GTAC	5 cut(s)	839, 1052, 2076, 2711, 3131
CviAII	CATG	9 cut(s)	977, 1077, 1163, 1249, 1322, 1498, 2361, 2401, 2791
CviQI	GTAC	5 cut(s)	839, 1052, 2076, 2711, 3131
DpnI	GATC	9 cut(s)	297, 337, 570, 732, 801, 1230, 1734, 2885, 3447
DpnII	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
DraIII	CACNNNGTG	1 cut(s)	1742
EaeI	YGGCCR	1 cut(s)	127
EagI	CGGCCG	1 cut(s)	127
Eam1104I	CTCTTC	2 cut(s)	2244, 2893
EarI	CTCTTC	2 cut(s)	2244, 2893
Ecl136II	GAGCTC	2 cut(s)	1327, 1819
EclXI	CGGCCG	1 cut(s)	127
Eco130I	CCWWGG	2 cut(s)	2704, 2790
Eco24I	GRGCYC	4 cut(s)	1329, 1684, 1821, 2335
Eco31I	GGTCTC	1 cut(s)	227
Eco32I	GATATC	2 cut(s)	1156, 2911
Eco47I	GGWCC	3 cut(s)	1220, 2210, 2805
Eco52I	CGGCCG	1 cut(s)	127
Eco53kI	GAGCTC	2 cut(s)	1327, 1819
Eco57I	CTGAAG	8 cut(s)	744, 1245, 1434, 1812, 1950, 1962, 2049, 3264
EcoICRI	GAGCTC	2 cut(s)	1327, 1819
EcoO109I	RGGNCCY	3 cut(s)	2210, 2805, 3383
EcoRI	GAATTC	1 cut(s)	2629
EcoRV	GATATC	2 cut(s)	1156, 2911
EcoT14I	CCWWGG	2 cut(s)	2704, 2790
EcoT38I	GRGCYC	4 cut(s)	1329, 1684, 1821, 2335
ErhI	CCWWGG	2 cut(s)	2704, 2790
FaeI	CATG	9 cut(s)	980, 1080, 1166, 1252, 1325, 1501, 2364, 2404, 2794
FalI	AAGNNNNNCTT	4 cut(s)	1935, 1967, 3159, 3191
FaqI	GGGAC	6 cut(s)	767, 1100, 1899, 2165, 2223, 2714
FatI	CATG	9 cut(s)	976, 1076, 1162, 1248, 1321, 1497, 2360, 2400, 2790
FblI	GTMKAC	1 cut(s)	3417
FriOI	GRGCYC	4 cut(s)	1329, 1684, 1821, 2335
FspAI	RTGCGCAY	1 cut(s)	3338
FspBI	CTAG	4 cut(s)	666, 1232, 1253, 3096
FspI	TGCGCA	1 cut(s)	3338
GlaI	GCGC	5 cut(s)	266, 2044, 2949, 3338, 3476
GsaI	CCCAGC	1 cut(s)	2268
GsuI	CTGGAG	3 cut(s)	636, 2757, 2858
HaeII	RGCGCY	1 cut(s)	3478
HaeIII	GGCC	6 cut(s)	129, 930, 1401, 1551, 3261, 3384
HapII	CCGG	6 cut(s)	496, 973, 2138, 2184, 2592, 3126
HgaI	GACGC	3 cut(s)	181, 1577, 1589
HhaI	GCGC	5 cut(s)	267, 2045, 2950, 3339, 3477
Hin1I	GRCGYC	2 cut(s)	192, 1600
Hin1II	CATG	9 cut(s)	980, 1080, 1166, 1252, 1325, 1501, 2364, 2404, 2794
Hin6I	GCGC	5 cut(s)	265, 2043, 2948, 3337, 3475
HinP1I	GCGC	5 cut(s)	265, 2043, 2948, 3337, 3475
HincII	GTYRAC	2 cut(s)	1926, 2230
HindII	GTYRAC	2 cut(s)	1926, 2230
HindIII	AAGCTT	3 cut(s)	523, 827, 1989
HpaI	GTTAAC	1 cut(s)	1926
HpaII	CCGG	6 cut(s)	496, 973, 2138, 2184, 2592, 3126
HphI	GGTGA	8 cut(s)	86, 290, 500, 809, 1032, 1199, 3171, 3278
Hpy166II	GTNNAC	5 cut(s)	1926, 2230, 3131, 3418, 3578
Hpy8I	GTNNAC	5 cut(s)	1926, 2230, 3131, 3418, 3578
Hpy99I	CGWCG	5 cut(s)	55, 111, 197, 3120, 3462
HpyCH4III	ACNGT	7 cut(s)	454, 2012, 2203, 3052, 3208, 3415, 3442
HpyCH4IV	ACGT	1 cut(s)	3574
HpySE526I	ACGT	1 cut(s)	3574
Hsp92I	GRCGYC	2 cut(s)	192, 1600
Hsp92II	CATG	9 cut(s)	980, 1080, 1166, 1252, 1325, 1501, 2364, 2404, 2794
HspAI	GCGC	5 cut(s)	265, 2043, 2948, 3337, 3475
KpnI	GGTACC	1 cut(s)	2714
KspAI	GTTAAC	1 cut(s)	1926
Kzo9I	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
LmnI	GCTCC	7 cut(s)	223, 874, 1300, 1824, 2141, 2738, 2877
MaeI	CTAG	4 cut(s)	666, 1232, 1253, 3096
MaeII	ACGT	1 cut(s)	3574
MaeIII	GTNAC	6 cut(s)	65, 448, 2254, 2270, 2356, 2699
MalI	GATC	9 cut(s)	297, 337, 570, 732, 801, 1230, 1734, 2885, 3447
MbiI	CCGCTC	1 cut(s)	877
MboI	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
MflI	RGATCY	2 cut(s)	568, 1228
MhlI	GDGCHC	7 cut(s)	1329, 1516, 1684, 1747, 1821, 2335, 3296
MlyI	GAGTC	1 cut(s)	437
MmeI	TCCRAC	4 cut(s)	273, 861, 1763, 3071
MroXI	GAANNNNTTC	1 cut(s)	1947
MseI	TTAA	5 cut(s)	783, 1182, 1925, 2724, 3437
MslI	CAYNNNNRTG	3 cut(s)	1149, 1212, 2322
MspA1I	CMGCKG	3 cut(s)	543, 2261, 2678
MspCI	CTTAAG	1 cut(s)	782
MspI	CCGG	6 cut(s)	496, 973, 2138, 2184, 2592, 3126
Mva1269I	GAATGC	3 cut(s)	180, 553, 700
MvnI	CGCG	3 cut(s)	267, 2043, 2950
NciI	CCSGG	3 cut(s)	497, 2139, 2184
NcoI	CCATGG	1 cut(s)	2790
NdeII	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
NheI	GCTAGC	1 cut(s)	1252
NlaIII	CATG	9 cut(s)	980, 1080, 1166, 1252, 1325, 1501, 2364, 2404, 2794
NlaIV	GGNNCC	4 cut(s)	372, 2211, 2712, 3286
NmeAIII	GCCGAG	1 cut(s)	105
NmuCI	GTSAC	3 cut(s)	65, 2356, 2699
NsbI	TGCGCA	1 cut(s)	3338
NspI	RCATGY	2 cut(s)	980, 2364
PaeI	GCATGC	1 cut(s)	980
PagI	TCATGA	2 cut(s)	1162, 1321
PciI	ACATGT	1 cut(s)	2360
PctI	GAATGC	3 cut(s)	180, 553, 700
PdmI	GAANNNNTTC	1 cut(s)	1947
PflMI	CCANNNNNTGG	3 cut(s)	816, 2843, 3235
PfoI	TCCNGGA	1 cut(s)	2874
PleI	GAGTC	1 cut(s)	436
PpsI	GAGTC	1 cut(s)	436
PpuMI	RGGWCCY	2 cut(s)	2210, 2805
PscI	ACATGT	1 cut(s)	2360
Psp124BI	GAGCTC	2 cut(s)	1329, 1821
Psp5II	RGGWCCY	2 cut(s)	2210, 2805
PspFI	CCCAGC	1 cut(s)	2264
PspN4I	GGNNCC	4 cut(s)	372, 2211, 2712, 3286
PspPI	GGNCC	4 cut(s)	1220, 2210, 2805, 3383
PspPPI	RGGWCCY	2 cut(s)	2210, 2805
PsrI	GAACNNNNNNTAC	2 cut(s)	1743, 1775
PstI	CTGCAG	1 cut(s)	3391
PstNI	CAGNNNCTG	2 cut(s)	2264, 2881
PsuI	RGATCY	2 cut(s)	568, 1228
RsaI	GTAC	5 cut(s)	840, 1053, 2077, 2712, 3132
RsaNI	GTAC	5 cut(s)	839, 1052, 2076, 2711, 3131
RseI	CAYNNNNRTG	3 cut(s)	1149, 1212, 2322
SacI	GAGCTC	2 cut(s)	1329, 1821
SaqAI	TTAA	5 cut(s)	783, 1182, 1925, 2724, 3437
Sau3AI	GATC	9 cut(s)	295, 335, 568, 730, 799, 1228, 1732, 2883, 3445
Sau96I	GGNCC	4 cut(s)	1220, 2210, 2805, 3383
ScaI	AGTACT	1 cut(s)	1053
SchI	GAGTC	1 cut(s)	437
SduI	GDGCHC	7 cut(s)	1329, 1516, 1684, 1747, 1821, 2335, 3296
SfcI	CTRYAG	7 cut(s)	1024, 1239, 1578, 2894, 3387, 3414, 3421
SinI	GGWCC	3 cut(s)	1220, 2210, 2805
SmiMI	CAYNNNNRTG	3 cut(s)	1149, 1212, 2322
SmlI	CTYRAG	5 cut(s)	782, 1169, 1507, 1619, 3500
SmoI	CTYRAG	5 cut(s)	782, 1169, 1507, 1619, 3500
SpeI	ACTAGT	1 cut(s)	665
SphI	GCATGC	1 cut(s)	980
SspI	AATATT	4 cut(s)	1937, 1959, 2055, 2934
SspMI	CTAG	4 cut(s)	666, 1232, 1253, 3096
SstI	GAGCTC	2 cut(s)	1329, 1821
StyI	CCWWGG	2 cut(s)	2704, 2790
TaaI	ACNGT	7 cut(s)	454, 2012, 2203, 3052, 3208, 3415, 3442
TaiI	ACGT	1 cut(s)	3577
TaqI	TCGA	8 cut(s)	109, 802, 964, 1003, 1389, 1908, 2335, 3118
TatI	WGTACW	1 cut(s)	1051
TauI	GCSGC	7 cut(s)	122, 192, 270, 543, 2264, 2681, 2953
Tru1I	TTAA	5 cut(s)	783, 1182, 1925, 2724, 3437
Tru9I	TTAA	5 cut(s)	783, 1182, 1925, 2724, 3437
TscAI	CASTG	5 cut(s)	2017, 2443, 2490, 2748, 3367
TseFI	GTSAC	3 cut(s)	65, 2356, 2699
Tsp45I	GTSAC	3 cut(s)	65, 2356, 2699
TspDTI	ATGAA	7 cut(s)	257, 1061, 1620, 1624, 1751, 2262, 3191
TspGWI	ACGGA	4 cut(s)	137, 531, 2641, 3446
TspRI	CASTG	5 cut(s)	2017, 2443, 2490, 2748, 3367
Van91I	CCANNNNNTGG	3 cut(s)	816, 2843, 3235
Vha464I	CTTAAG	1 cut(s)	782
VpaK11BI	GGWCC	3 cut(s)	1220, 2210, 2805
XapI	RAATTY	8 cut(s)	895, 1017, 1068, 1100, 2037, 2629, 3061, 3320
XceI	RCATGY	2 cut(s)	980, 2364
XcmI	CCANNNNNNNNNTGG	1 cut(s)	308
XmiI	GTMKAC	1 cut(s)	3417
XmnI	GAANNNNTTC	1 cut(s)	1947
XspI	CTAG	4 cut(s)	666, 1232, 1253, 3096
ZrmI	AGTACT	1 cut(s)	1053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.