MD04G1041900.v1.1

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009654
Physical Location & Seq
Reverse (-)
4964686 .. 4969045
4360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1041900.v1.1.491

Sequence Viewer

Length: 609 bp
ATGAACGTAGAAGCTTTAGAACCGTATATAGGCATGGAGTTCAACTCAAGAGATGAAGCTAGAGAATTTTATGTTGCATATGGCAGACGCTCCGGTTTCACCGTACGGATACACCACAATCGTCGTTCACGAGTAAATAATCAGATTATTGGTCAAGATTTTGTTTGCTCAAAAGAAGGCTTTCGAGCAAAAAAGTATGTGCACAGAAAAGACAGAGTTCTTCCTCCACCCCCAGTAACCCGTGAGGGCTGTCAAGCTATGATAAGGCTAGCTCTAAGAGATGGACCGAAGTGGGTAGTCACAAAATTTGTTAAAGAGCATAATCACAAACTAATGAGTCCCAGCAAGGTTCCATGGCGGGGATCAGGAAAGCACTTAGCAAGTGAGGATGAGAAGGATAAAAGAATCCGAGAGCTGTCCCTTGAGCTGTACAACGAGAGGCAAAAATGTAAGCGACGTTGTGCTGCATATGAAGAACAGCTAAATATGATCTTGAAAGATTTGGAAAAACACACAGAGCACATGTCAAAAAAAGTTTCGGATACAGTTCAGAGCATAAAAGAAATTGAGGAGGAACAATCAGAGGACTCGGATAGCGGATGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

203

Amino Acids

23.75

Weight (kDa)

9.21

Isoelectric Point (pI)

64.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 22 - 112 3.7e-26 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 358, 597
AclWI GGATC 1 cut(s) 370
AcsI RAATTY 2 cut(s) 65, 305
AfaI GTAC 2 cut(s) 105, 431
AfiI CCNNNNNNNGG 2 cut(s) 29, 359
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 2 cut(s) 43, 496
AluBI AGCT 7 cut(s) 14, 59, 257, 272, 415, 427, 481
AluI AGCT 7 cut(s) 14, 59, 257, 272, 415, 427, 481
Alw21I GWGCWC 2 cut(s) 204, 522
Alw44I GTGCAC 1 cut(s) 200
AlwI GGATC 1 cut(s) 370
ApaLI GTGCAC 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 2 cut(s) 65, 305
Asp700I GAANNNNTTC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 268
AvaII GGWCC 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 204
BauI CACGAG 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 204, 522
BbvI GCAGC 1 cut(s) 451
BccI CCATC 2 cut(s) 275, 594
BciVI GTATCC 2 cut(s) 102, 535
BfaI CTAG 2 cut(s) 60, 269
BfuI GTATCC 2 cut(s) 102, 535
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 227
BmtI GCTAGC 1 cut(s) 272
BmuI ACTGGG 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpuEI CTTGAG 2 cut(s) 31, 443
BsaJI CCNNGG 1 cut(s) 353
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 359
Bse1I ACTGG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 353
BseGI GGATG 2 cut(s) 394, 605
BseLI CCNNNNNNNGG 2 cut(s) 29, 359
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 204
BseXI GCAGC 1 cut(s) 451
BseYI CCCAGC 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 204, 522
BsiSI CCGG 1 cut(s) 93
BsiWI CGTACG 1 cut(s) 103
BslFI GGGAC 2 cut(s) 324, 403
BslI CCNNNNNNNGG 2 cut(s) 29, 359
BsmFI GGGAC 2 cut(s) 324, 403
Bsp1286I GDGCHC 2 cut(s) 204, 522
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 2 cut(s) 362, 489
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 2 cut(s) 358, 597
BspLI GGNNCC 1 cut(s) 351
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 370
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 353
BssMI GATC 2 cut(s) 362, 489
BssSI CACGAG 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 24, 103, 547
BstAUI TGTACA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 275, 376
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 394, 605
BstKTI GATC 2 cut(s) 365, 492
BstMBI GATC 2 cut(s) 362, 489
BstNSI RCATGY 1 cut(s) 526
BstSLI GKGCMC 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 451
BsuI GTATCC 2 cut(s) 102, 535
BtgI CCRYGG 1 cut(s) 353
BtsCI GGATG 2 cut(s) 394, 605
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 284
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 104, 430
CviAII CATG 3 cut(s) 34, 354, 523
CviQI GTAC 2 cut(s) 104, 430
DdeI CTNAG 2 cut(s) 275, 376
DpnI GATC 2 cut(s) 364, 491
DpnII GATC 2 cut(s) 362, 489
Eco130I CCWWGG 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 3 cut(s) 37, 357, 526
FaqI GGGAC 2 cut(s) 324, 403
FatI CATG 3 cut(s) 33, 353, 522
FauI CCCGC 1 cut(s) 351
FauNDI CATATG 2 cut(s) 79, 469
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 465
FspBI CTAG 2 cut(s) 60, 269
GluI GCNGC 1 cut(s) 465
GsaI CCCAGC 1 cut(s) 345
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 96
Hin1II CATG 3 cut(s) 37, 357, 526
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 337, 405, 587
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 128, 202
Hpy188I TCNGA 6 cut(s) 144, 410, 541, 552, 583, 592
Hpy188III TCNNGA 5 cut(s) 48, 129, 155, 366, 493
Hpy8I GTNNAC 2 cut(s) 128, 202
Hpy99I CGWCG 2 cut(s) 126, 459
HpyAV CCTTC 2 cut(s) 170, 388
HpyCH4III ACNGT 3 cut(s) 24, 103, 547
HpyCH4IV ACGT 2 cut(s) 6, 457
HpyCH4V TGCA 3 cut(s) 77, 202, 467
HpyF3I CTNAG 2 cut(s) 275, 376
HpySE526I ACGT 2 cut(s) 6, 457
Hsp92II CATG 3 cut(s) 37, 357, 526
Kzo9I GATC 2 cut(s) 362, 489
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 4 cut(s) 106, 246, 351, 355
Lsp1109I GCAGC 1 cut(s) 451
MaeI CTAG 2 cut(s) 60, 269
MaeII ACGT 2 cut(s) 6, 457
MaeIII GTNAC 2 cut(s) 235, 298
MalI GATC 2 cut(s) 364, 491
MboI GATC 2 cut(s) 362, 489
MboII GAAGA 2 cut(s) 212, 485
MhlI GDGCHC 2 cut(s) 204, 522
MluCI AATT 3 cut(s) 65, 305, 564
MlyI GAGTC 2 cut(s) 346, 581
MnlI CCTC 7 cut(s) 234, 238, 379, 432, 562, 565, 577
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 2 cut(s) 312, 607
MspI CCGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 353
NdeI CATATG 2 cut(s) 79, 469
NdeII GATC 2 cut(s) 362, 489
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 3 cut(s) 37, 357, 526
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 298
NspI RCATGY 1 cut(s) 526
PciI ACATGT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 405
Pfl23II CGTACG 1 cut(s) 103
PkrI GCNGC 1 cut(s) 466
PleI GAGTC 2 cut(s) 345, 581
PpsI GAGTC 2 cut(s) 345, 581
PscI ACATGT 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 341
PspLI CGTACG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 284
RsaI GTAC 2 cut(s) 105, 431
RsaNI GTAC 2 cut(s) 104, 430
SaqAI TTAA 2 cut(s) 312, 607
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 2 cut(s) 362, 489
Sau96I GGNCC 1 cut(s) 284
SchI GAGTC 2 cut(s) 346, 581
SduI GDGCHC 2 cut(s) 204, 522
SinI GGWCC 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 46, 422
SmoI CTYRAG 2 cut(s) 46, 422
Sse9I AATT 3 cut(s) 65, 305, 564
SsiI CCGC 2 cut(s) 358, 597
SspMI CTAG 2 cut(s) 60, 269
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 3 cut(s) 24, 103, 547
TaiI ACGT 2 cut(s) 9, 460
TaqI TCGA 1 cut(s) 184
TaqII GACCGA 1 cut(s) 301
TasI AATT 3 cut(s) 65, 305, 564
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 1 cut(s) 405
Tru1I TTAA 2 cut(s) 312, 607
Tru9I TTAA 2 cut(s) 312, 607
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 17, 69, 486
TspGWI ACGGA 1 cut(s) 121
VneI GTGCAC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 2 cut(s) 65, 305
XceI RCATGY 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 60, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.