MD04G1062000.v1.1

Belongs to the eukaryotic ribosomal protein eL15 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005376
Physical Location & Seq
Forward (+)
8176477 .. 8178741
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1062000.v1.1.491

Sequence Viewer

Length: 711 bp
ATGTCCAGTTTTCACCACAAACCCTCACTCTGCGCTTCGAAAACCCTAGCCCCGACTGCCCAACCCTGCGCGTCGCAGCTCATTTCTGAACCGGCCATGGGGGCTTACAAGTACGTATCCGAGATATGGAGGAAGAAGCAGTCTGATGTGATGAGGTTCCTCCAGAGGGTCCGCTGCTGGGAGTATCGCCAGCATCCTTCGATTGTCCGAGTTACAAGACCCACACGCCCTGATAAGGCGCGTCGTTTGGGTTACAAGGCCAAGCAGGGTTACGTTGTTTACCGTGTCCGTGTGAGGCGTGGTGGGCGCAAGAGGCCTGTTTCCAAGGGTATTGTATATGGAAAGCCCACAAACCAGGGTGTGACACAACTCAAGTTCCAGCGTAGCAAGAGGTCTGTTGCTGAGGAGCGTGCTGGGCGCAAGTTGGGAGGCCTCAGGGTTCTCAACTCATACTGGCTCAATGAGGATTCGACCTACAAGTACTTTGAGGTCATCATGGTTGATGTTGCTCACAATGCCATCAGGAACGACCCAAGAATCAACTGGCTCTGCAACCCTGTTCACAAGCACAGAGAGCTTCGTGGTCTTACTTCTGCTGGAAAGAAATACAGGGGTCTGCGTGGAAAGGGTCATCTGTACCACAAGAACCGACCTTCTCGCAGGGCAACATGGAAGAGAAATACCACCCTTTCACTCCGTCGTTACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L15e

Protein Analysis

237

Amino Acids

27.65

Weight (kDa)

11.23

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L15e PF00827 34 - 222 2.9e-91 Ribosomal L15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 71, 241
AciI CCGC 1 cut(s) 172
AcoI YGGCCR 1 cut(s) 93
AfaI GTAC 3 cut(s) 113, 482, 638
AfiI CCNNNNNNNGG 5 cut(s) 98, 126, 166, 178, 235
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 79, 577
AluI AGCT 2 cut(s) 79, 577
AoxI GGCC 4 cut(s) 93, 258, 314, 430
ApeKI GCWGC 2 cut(s) 76, 174
AspLEI GCGC 5 cut(s) 35, 71, 241, 309, 420
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 5
AsuII TTCGAA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 402
BbvI GCAGC 2 cut(s) 88, 161
BccI CCATC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 356
BciVI GTATCC 1 cut(s) 127
BfaI CTAG 1 cut(s) 47
BfuI GTATCC 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 2 cut(s) 77, 175
BlsI GCNGC 2 cut(s) 78, 176
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 158, 170
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 96, 324, 355
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 126, 166, 178, 235
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 6, 458, 548
Bse21I CCTNAGG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 96, 324, 355
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 5 cut(s) 98, 126, 166, 178, 235
BseMII CTCAG 2 cut(s) 393, 448
BseNI ACTGG 3 cut(s) 6, 458, 548
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 88, 161
BseYI CCCAGC 2 cut(s) 177, 413
Bsh1236I CGCG 2 cut(s) 71, 241
BshFI GGCC 4 cut(s) 95, 260, 316, 432
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 5 cut(s) 98, 126, 166, 178, 235
BsnI GGCC 4 cut(s) 95, 260, 316, 432
Bsp119I TTCGAA 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 1 cut(s) 172
BspANI GGCC 4 cut(s) 95, 260, 316, 432
BspCNI CTCAG 2 cut(s) 394, 447
BspFNI CGCG 2 cut(s) 71, 241
BspLI GGNNCC 2 cut(s) 158, 170
BspT104I TTCGAA 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 3 cut(s) 6, 458, 548
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 96, 324, 355
BssT1I CCWWGG 2 cut(s) 96, 324
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 284, 707
Bst6I CTCTTC 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 115
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 191, 411
BstDEI CTNAG 2 cut(s) 402, 434
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 2 cut(s) 71, 241
BstHHI GCGC 5 cut(s) 35, 71, 241, 309, 420
BstMWI GCNNNNNNNGC 7 cut(s) 56, 101, 304, 313, 415, 515, 574
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstSNI TACGTA 1 cut(s) 115
BstUI CGCG 2 cut(s) 71, 241
BstV1I GCAGC 2 cut(s) 88, 161
Bsu36I CCTNAGG 1 cut(s) 434
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 4 cut(s) 95, 260, 316, 432
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 191, 411
CfoI GCGC 5 cut(s) 35, 71, 241, 309, 420
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 2 cut(s) 60, 230
Csp6I GTAC 3 cut(s) 112, 481, 637
CviAII CATG 3 cut(s) 97, 496, 669
CviQI GTAC 3 cut(s) 112, 481, 637
DdeI CTNAG 2 cut(s) 402, 434
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
Eco105I TACGTA 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 96, 324
Eco147I AGGCCT 2 cut(s) 316, 432
Eco47I GGWCC 1 cut(s) 169
Eco81I CCTNAGG 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 2 cut(s) 96, 324
ErhI CCWWGG 2 cut(s) 96, 324
FaeI CATG 3 cut(s) 100, 499, 672
FaiI YATR 7 cut(s) 98, 127, 337, 339, 451, 497, 670
FatI CATG 3 cut(s) 96, 495, 668
Fnu4HI GCNGC 2 cut(s) 77, 175
FokI GGATG 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 77, 175
FspBI CTAG 1 cut(s) 47
GlaI GCGC 5 cut(s) 34, 70, 240, 308, 419
GluI GCNGC 2 cut(s) 77, 175
GsaI CCCAGC 2 cut(s) 181, 417
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 4 cut(s) 95, 260, 316, 432
HapII CCGG 1 cut(s) 92
HgaI GACGC 2 cut(s) 60, 230
HhaI GCGC 5 cut(s) 35, 71, 241, 309, 420
Hin1II CATG 3 cut(s) 100, 499, 672
Hin6I GCGC 5 cut(s) 33, 69, 239, 307, 418
HinP1I GCGC 5 cut(s) 33, 69, 239, 307, 418
HinfI GANTC 2 cut(s) 467, 537
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 5
Hpy166II GTNNAC 2 cut(s) 280, 562
Hpy188I TCNGA 4 cut(s) 88, 121, 145, 209
Hpy188III TCNNGA 2 cut(s) 163, 523
Hpy8I GTNNAC 2 cut(s) 280, 562
Hpy99I CGWCG 3 cut(s) 76, 246, 702
HpyAV CCTTC 2 cut(s) 207, 663
HpyCH4III ACNGT 2 cut(s) 284, 707
HpyCH4IV ACGT 2 cut(s) 114, 273
HpyCH4V TGCA 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 101, 304, 313, 415, 515, 574
HpyF3I CTNAG 2 cut(s) 402, 434
HpySE526I ACGT 2 cut(s) 114, 273
Hsp92II CATG 3 cut(s) 100, 499, 672
HspAI GCGC 5 cut(s) 33, 69, 239, 307, 418
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 2 cut(s) 88, 161
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 47
MaeII ACGT 2 cut(s) 114, 273
MaeIII GTNAC 5 cut(s) 211, 251, 269, 361, 701
MboII GAAGA 2 cut(s) 145, 685
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 71, 241
MwoI GCNNNNNNNGC 7 cut(s) 56, 101, 304, 313, 415, 515, 574
NcoI CCATGG 1 cut(s) 96
NlaIII CATG 3 cut(s) 100, 499, 672
NlaIV GGNNCC 2 cut(s) 158, 170
NmuCI GTSAC 1 cut(s) 361
NspV TTCGAA 1 cut(s) 38
PceI AGGCCT 2 cut(s) 316, 432
PfeI GAWTC 2 cut(s) 467, 537
PkrI GCNGC 2 cut(s) 78, 176
Ppu21I YACGTR 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 2 cut(s) 177, 413
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 158, 170
PspPI GGNCC 1 cut(s) 169
RsaI GTAC 3 cut(s) 113, 482, 638
RsaNI GTAC 3 cut(s) 112, 481, 637
SatI GCNGC 2 cut(s) 77, 175
Sau96I GGNCC 1 cut(s) 169
ScaI AGTACT 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 356
SetI ASST 9 cut(s) 81, 117, 158, 276, 395, 476, 492, 579, 655
SfaNI GCATC 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SnaBI TACGTA 1 cut(s) 115
SseBI AGGCCT 2 cut(s) 316, 432
SsiI CCGC 1 cut(s) 172
SspMI CTAG 1 cut(s) 47
StuI AGGCCT 2 cut(s) 316, 432
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 2 cut(s) 96, 324
TaaI ACNGT 2 cut(s) 284, 707
TaiI ACGT 2 cut(s) 117, 276
TaqI TCGA 3 cut(s) 38, 200, 470
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 2 cut(s) 467, 537
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 2 cut(s) 76, 174
Tsp45I GTSAC 1 cut(s) 361
TspGWI ACGGA 2 cut(s) 278, 686
VpaK11BI GGWCC 1 cut(s) 169
XcmI CCANNNNNNNNNTGG 1 cut(s) 540
XspI CTAG 1 cut(s) 47
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.