MD04G1093700.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005742
Physical Location & Seq
Forward (+)
17052592 .. 17053047
456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1093700.v1.1.491

Sequence Viewer

Length: 456 bp
ATGTCTACCGAGAAGAACATATATAGATCGAAGAAAGCCGAGGATCGAGGTTTGCTGATGCTGAGGCTGGTAATCGGGAAGCTAGTGCACAAGAGTCTCTCGATCTTAGCATCTAGAGGCCCTCTCGATCACCATCGTCGTCACAATGATTTCCATGAAGAATCGGAAGTGACAACGGTGGTGCCAGGAGACATTAAAGAAGGATACTTTGCAGTTTTTGCTGTCAAGGGGAATGAAGCAGAGAGGTTTGTGGTCAAATTGGAGTCGTTGAGTAACCCTGAATTTTTGAGGTTACTGGAAGAGGCCAAGGAAGAGTATGGATTTGATCAGAAGGGTGCTCTTGTAGTTCCTTGCAGGCCTGAGGAATTGCGTAAGATTTTACAAAACTGCAGGAGGAATAAGAGGAGTAGTAGAACATGTATTAATATTAATTTTACAGTTATACAAGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

152

Amino Acids

17.4

Weight (kDa)

9.19

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 46 - 127 1e-15 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 5
AclWI GGATC 1 cut(s) 51
AcsI RAATTY 1 cut(s) 281
AflIII ACRYGT 1 cut(s) 416
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 2 cut(s) 90, 340
Alw26I GTCTC 2 cut(s) 101, 183
Alw44I GTGCAC 1 cut(s) 86
AlwI GGATC 1 cut(s) 51
AoxI GGCC 3 cut(s) 118, 303, 356
ApaLI GTGCAC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 281
AseI ATTAAT 2 cut(s) 423, 429
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 360
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 181
Bbv12I GWGCWC 2 cut(s) 90, 340
BbvCI CCTCAGC 1 cut(s) 62
BccI CCATC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 186
BciVI GTATCC 1 cut(s) 197
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 101, 183
BfaI CTAG 2 cut(s) 83, 114
BfmI CTRYAG 1 cut(s) 388
BfuI GTATCC 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 48, 119
BplI GAGNNNNNCTC 2 cut(s) 108, 140
Bpu10I CCTNAGC 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 39, 306
Bse1I ACTGG 1 cut(s) 300
Bse21I CCTNAGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 39, 306
BseJI GATNNNNATC 1 cut(s) 132
BseMII CTCAG 2 cut(s) 53, 351
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 418
BseSI GKGCMC 1 cut(s) 90
BshFI GGCC 3 cut(s) 120, 305, 358
BshNI GGYRCC 1 cut(s) 181
BsiHKAI GWGCWC 2 cut(s) 90, 340
BsmAI GTCTC 2 cut(s) 101, 183
BsnI GGCC 3 cut(s) 120, 305, 358
Bsp1286I GDGCHC 2 cut(s) 90, 340
Bsp143I GATC 5 cut(s) 26, 43, 102, 127, 325
BspANI GGCC 3 cut(s) 120, 305, 358
BspCNI CTCAG 2 cut(s) 54, 352
BspLI GGNNCC 1 cut(s) 183
BspMAI CTGCAG 1 cut(s) 392
BspPI GGATC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 181
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 39, 306
BssMI GATC 5 cut(s) 26, 43, 102, 127, 325
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 178, 439
Bst6I CTCTTC 2 cut(s) 294, 306
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 3 cut(s) 62, 106, 360
BstKTI GATC 5 cut(s) 29, 46, 105, 130, 328
BstMAI GTCTC 2 cut(s) 101, 183
BstMBI GATC 5 cut(s) 26, 43, 102, 127, 325
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 388
BstSLI GKGCMC 1 cut(s) 90
Bsu36I CCTNAGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 3 cut(s) 120, 305, 358
Cac8I GCNNGC 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 119
CviAII CATG 2 cut(s) 155, 417
CviJI RGCY 6 cut(s) 38, 67, 82, 120, 305, 358
CviKI_1 RGCY 6 cut(s) 38, 67, 82, 120, 305, 358
DdeI CTNAG 3 cut(s) 62, 106, 360
DpnI GATC 5 cut(s) 28, 45, 104, 129, 327
DpnII GATC 5 cut(s) 26, 43, 102, 127, 325
Eam1104I CTCTTC 2 cut(s) 294, 306
EarI CTCTTC 2 cut(s) 294, 306
Eco130I CCWWGG 1 cut(s) 306
Eco147I AGGCCT 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 158, 420
FaiI YATR 7 cut(s) 20, 22, 24, 156, 318, 418, 443
FatI CATG 2 cut(s) 154, 416
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 5
FspBI CTAG 2 cut(s) 83, 114
HaeIII GGCC 3 cut(s) 120, 305, 358
Hin1II CATG 2 cut(s) 158, 420
HinfI GANTC 3 cut(s) 94, 161, 263
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 6, 88
Hpy188I TCNGA 2 cut(s) 166, 330
Hpy188III TCNNGA 4 cut(s) 76, 100, 114, 125
Hpy8I GTNNAC 2 cut(s) 6, 88
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 2 cut(s) 194, 325
HpyCH4III ACNGT 2 cut(s) 178, 439
HpyCH4V TGCA 4 cut(s) 88, 212, 354, 390
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 3 cut(s) 62, 106, 360
Hsp92II CATG 2 cut(s) 158, 420
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 5 cut(s) 26, 43, 102, 127, 325
LpnPI CCDG 8 cut(s) 53, 171, 198, 281, 291, 340, 372, 376
LweI GCATC 2 cut(s) 48, 119
MaeI CTAG 2 cut(s) 83, 114
MaeIII GTNAC 4 cut(s) 140, 169, 272, 291
MalI GATC 5 cut(s) 28, 45, 104, 129, 327
MboI GATC 5 cut(s) 26, 43, 102, 127, 325
MboII GAAGA 5 cut(s) 25, 43, 170, 311, 323
MhlI GDGCHC 2 cut(s) 90, 340
MluCI AATT 4 cut(s) 257, 281, 365, 430
MlyI GAGTC 2 cut(s) 103, 272
MseI TTAA 3 cut(s) 195, 423, 429
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 218
NdeII GATC 5 cut(s) 26, 43, 102, 127, 325
NlaIII CATG 2 cut(s) 158, 420
NlaIV GGNNCC 1 cut(s) 183
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 2 cut(s) 140, 169
NspI RCATGY 1 cut(s) 420
PceI AGGCCT 1 cut(s) 358
PciI ACATGT 1 cut(s) 416
PfeI GAWTC 1 cut(s) 161
PleI GAGTC 2 cut(s) 102, 271
PpsI GAGTC 2 cut(s) 102, 271
PscI ACATGT 1 cut(s) 416
PshBI ATTAAT 2 cut(s) 423, 429
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 119
PstI CTGCAG 1 cut(s) 392
SaqAI TTAA 3 cut(s) 195, 423, 429
Sau3AI GATC 5 cut(s) 26, 43, 102, 127, 325
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 2 cut(s) 103, 272
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 2 cut(s) 90, 340
SetI ASST 4 cut(s) 52, 84, 248, 293
SfaNI GCATC 2 cut(s) 48, 119
SfcI CTRYAG 1 cut(s) 388
Sse9I AATT 4 cut(s) 257, 281, 365, 430
SseBI AGGCCT 1 cut(s) 358
SspI AATATT 1 cut(s) 427
SspMI CTAG 2 cut(s) 83, 114
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 178, 439
TaqI TCGA 4 cut(s) 29, 46, 101, 126
TasI AATT 4 cut(s) 257, 281, 365, 430
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 3 cut(s) 195, 423, 429
Tru9I TTAA 3 cut(s) 195, 423, 429
TseFI GTSAC 2 cut(s) 140, 169
Tsp45I GTSAC 2 cut(s) 140, 169
TspDTI ATGAA 2 cut(s) 171, 249
VneI GTGCAC 1 cut(s) 86
VspI ATTAAT 2 cut(s) 423, 429
XapI RAATTY 1 cut(s) 281
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 83, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.