pycom04g08900

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005742
Physical Location & Seq
Forward (+)
11017469 .. 11017900
432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08900.1

Sequence Viewer

Length: 432 bp
ATGTCTACGGAGAAGAACTGCGGATCGAAGAAAGCCGAGGATCGAGGATTGCTGATGCTGAGGCTACTCATTGGGAAGCTAGTGCACAAGGGTCTCTCAATCTTGGCATCAAGAGGCCTTCATGGTCACCACCATCGTCACAATGATTTCAACGTAGAATCGGAAGCTACGATGGTGGTGCCGGAAGATGTTAAAGATGGACACTTTGCAGTTTTTGCTGTCAAGGGGAATGAAGCAGAGAGGTTTGTGGTTAAATTGGAGTCGTTGAGTAACCCTGAATTTTTGAGGTTATTGGAAGAGGCCAAGGAAGAGTATGGATTTGATCAGAAGGGTGCTCTTGCAGTTCCTTGCAGGCCTCGAGAATTGCAGAAAATTCTACAAAGCTGCAGGAGGAAAAATAATATTAATTTTACAGTTACACAAGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

144

Amino Acids

16.17

Weight (kDa)

8.81

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 52 - 127 2.2e-16 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 21
AclWI GGATC 2 cut(s) 31, 48
AcsI RAATTY 2 cut(s) 278, 372
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 3 cut(s) 79, 167, 384
AluI AGCT 3 cut(s) 79, 167, 384
Alw21I GWGCWC 2 cut(s) 87, 337
Alw26I GTCTC 1 cut(s) 98
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 2 cut(s) 31, 48
Ama87I CYCGRG 1 cut(s) 357
AoxI GGCC 3 cut(s) 115, 300, 353
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 2 cut(s) 278, 372
AseI ATTAAT 1 cut(s) 405
AsuHPI GGTGA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 178
Bbv12I GWGCWC 2 cut(s) 87, 337
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 371
BccI CCATC 3 cut(s) 141, 166, 191
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciVI GTATCC 1 cut(s) 419
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 2 cut(s) 80, 430
BfmI CTRYAG 1 cut(s) 385
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 180
BmsI GCATC 2 cut(s) 45, 116
Bpu10I CCTNAGC 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 36, 303
BseDI CCNNGG 2 cut(s) 36, 303
BseMII CTCAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 371
BshFI GGCC 3 cut(s) 117, 302, 355
BshNI GGYRCC 1 cut(s) 178
BsiHKAI GWGCWC 2 cut(s) 87, 337
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 3 cut(s) 117, 302, 355
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 357
Bsp1286I GDGCHC 2 cut(s) 87, 337
Bsp143I GATC 3 cut(s) 23, 40, 322
BspACI CCGC 1 cut(s) 21
BspANI GGCC 3 cut(s) 117, 302, 355
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 389
BspPI GGATC 2 cut(s) 31, 48
BspT107I GGYRCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 36, 303
BssMI GATC 3 cut(s) 23, 40, 322
BssT1I CCWWGG 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 2 cut(s) 291, 303
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 59
BstEII GGTNACC 1 cut(s) 125
BstKTI GATC 3 cut(s) 26, 43, 325
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 23, 40, 322
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstPI GGTNACC 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 385
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 371
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 3 cut(s) 117, 302, 355
Cac8I GCNNGC 1 cut(s) 353
CviAII CATG 1 cut(s) 122
CviJI RGCY 8 cut(s) 35, 64, 79, 117, 167, 302, 355, 384
CviKI_1 RGCY 8 cut(s) 35, 64, 79, 117, 167, 302, 355, 384
DdeI CTNAG 1 cut(s) 59
DpnI GATC 3 cut(s) 25, 42, 324
DpnII GATC 3 cut(s) 23, 40, 322
Eam1104I CTCTTC 2 cut(s) 291, 303
EarI CTCTTC 2 cut(s) 291, 303
Eco130I CCWWGG 1 cut(s) 303
Eco147I AGGCCT 2 cut(s) 117, 355
Eco31I GGTCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 357
Eco91I GGTNACC 1 cut(s) 125
EcoO65I GGTNACC 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 125
FaiI YATR 2 cut(s) 123, 315
FatI CATG 1 cut(s) 121
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 385
FspBI CTAG 2 cut(s) 80, 430
GluI GCNGC 1 cut(s) 385
HaeIII GGCC 3 cut(s) 117, 302, 355
HapII CCGG 1 cut(s) 182
Hin1II CATG 1 cut(s) 125
HinfI GANTC 2 cut(s) 158, 260
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 2 cut(s) 6, 85
Hpy188I TCNGA 2 cut(s) 163, 327
Hpy188III TCNNGA 2 cut(s) 111, 359
Hpy8I GTNNAC 2 cut(s) 6, 85
HpyAV CCTTC 2 cut(s) 128, 322
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 6 cut(s) 85, 209, 341, 351, 367, 387
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 3 cut(s) 23, 40, 322
LpnPI CCDG 4 cut(s) 195, 288, 337, 373
Lsp1109I GCAGC 1 cut(s) 371
LweI GCATC 2 cut(s) 45, 116
MaeI CTAG 2 cut(s) 80, 430
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 4 cut(s) 125, 137, 269, 415
MalI GATC 3 cut(s) 25, 42, 324
MboI GATC 3 cut(s) 23, 40, 322
MboII GAAGA 5 cut(s) 25, 40, 197, 308, 320
MhlI GDGCHC 2 cut(s) 87, 337
MluCI AATT 5 cut(s) 254, 278, 362, 372, 406
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 9 cut(s) 31, 38, 54, 107, 234, 279, 292, 366, 384
MseI TTAA 3 cut(s) 192, 252, 405
MspI CCGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 215
NdeII GATC 3 cut(s) 23, 40, 322
NlaIII CATG 1 cut(s) 125
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 125, 137
PaeR7I CTCGAG 1 cut(s) 357
PceI AGGCCT 2 cut(s) 117, 355
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 1 cut(s) 386
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 405
PspEI GGTNACC 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 180
PstI CTGCAG 1 cut(s) 389
SaqAI TTAA 3 cut(s) 192, 252, 405
SatI GCNGC 1 cut(s) 385
Sau3AI GATC 3 cut(s) 23, 40, 322
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 87, 337
SetI ASST 6 cut(s) 81, 156, 169, 245, 290, 386
SfaNI GCATC 2 cut(s) 45, 116
SfcI CTRYAG 1 cut(s) 385
Sfr274I CTCGAG 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 5 cut(s) 254, 278, 362, 372, 406
SseBI AGGCCT 2 cut(s) 117, 355
SsiI CCGC 1 cut(s) 21
SspI AATATT 1 cut(s) 403
SspMI CTAG 2 cut(s) 80, 430
StuI AGGCCT 2 cut(s) 117, 355
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 1 cut(s) 156
TaqI TCGA 3 cut(s) 26, 43, 358
TasI AATT 5 cut(s) 254, 278, 362, 372, 406
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 3 cut(s) 192, 252, 405
Tru9I TTAA 3 cut(s) 192, 252, 405
TseFI GTSAC 2 cut(s) 125, 137
TseI GCWGC 1 cut(s) 384
Tsp45I GTSAC 2 cut(s) 125, 137
TspDTI ATGAA 2 cut(s) 110, 246
TspGWI ACGGA 1 cut(s) 23
VneI GTGCAC 1 cut(s) 83
VspI ATTAAT 1 cut(s) 405
XapI RAATTY 2 cut(s) 278, 372
XhoI CTCGAG 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 80, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.