MD12G1113400.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005742
Physical Location & Seq
Forward (+)
18052351 .. 18052800
450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1113400.v1.1.491

Sequence Viewer

Length: 450 bp
ATGTCTACGGAGAAGAACTGCGGATCGAAGAAAGCCGAGGATCGAGGTTTGATGATGCTGAGGCTGCTCATTGGGAAGCTAGTGCACAAGGGTCTCTCAATCTTGGCATCAAGAGGCCCTCACGATCACCACCCTCGTCACAATGATTTCGACGAAGATTCAGAAGTGGCGACGGTGGTGCCGAAAGACGTAAAAGAAGGTCACTTTACAGTTTTTGCAGTGAAGGGTAATGAAGCAGAGAGGTTTGTTGTTAAACTGGAGTCGTTGAGTAACCCTGAATTTTTGAGGTTATTGGAAGAGGCCAAGGAAGAGTACGGATTTGAGCAGAAAGGTGCTCTTGCAATTCCTTGCAGGCCTCAAGAATTGCAGAAAATCTTACAAACATGCAGGAGGAAGAAGAGTAGTAGAACATCTAATAATATTAATTTTGCAGTTATACAAGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

150

Amino Acids

16.86

Weight (kDa)

8.78

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 55 - 127 1.6e-16 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 21
AclWI GGATC 2 cut(s) 31, 48
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 1 cut(s) 314
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw21I GWGCWC 2 cut(s) 87, 337
Alw26I GTCTC 1 cut(s) 98
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 2 cut(s) 31, 48
AoxI GGCC 3 cut(s) 115, 300, 353
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 278
AseI ATTAAT 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 178
Bbv12I GWGCWC 2 cut(s) 87, 337
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciVI GTATCC 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 2 cut(s) 80, 448
BfuI GTATCC 1 cut(s) 437
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 180
BmsI GCATC 2 cut(s) 45, 116
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 36, 303
Bse1I ACTGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 36, 303
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 51
BshFI GGCC 3 cut(s) 117, 302, 355
BshNI GGYRCC 1 cut(s) 178
BsiHKAI GWGCWC 2 cut(s) 87, 337
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 3 cut(s) 117, 302, 355
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 87, 337
Bsp143I GATC 3 cut(s) 23, 40, 124
BspACI CCGC 1 cut(s) 21
BspANI GGCC 3 cut(s) 117, 302, 355
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 2 cut(s) 31, 48
BspT107I GGYRCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 2 cut(s) 36, 303
BssMI GATC 3 cut(s) 23, 40, 124
BssT1I CCWWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 175, 211
Bst6I CTCTTC 3 cut(s) 291, 303, 392
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 59
BstKTI GATC 3 cut(s) 26, 43, 127
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 23, 40, 124
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 51
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 3 cut(s) 117, 302, 355
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 313
CviAII CATG 1 cut(s) 384
CviJI RGCY 6 cut(s) 35, 64, 79, 117, 302, 355
CviKI_1 RGCY 6 cut(s) 35, 64, 79, 117, 302, 355
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 59
DpnI GATC 3 cut(s) 25, 42, 126
DpnII GATC 3 cut(s) 23, 40, 124
Eam1104I CTCTTC 3 cut(s) 291, 303, 392
EarI CTCTTC 3 cut(s) 291, 303, 392
Eco130I CCWWGG 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 98
EcoO109I RGGNCCY 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 387
FaiI YATR 2 cut(s) 385, 437
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FatI CATG 1 cut(s) 383
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 65
FspBI CTAG 2 cut(s) 80, 448
GluI GCNGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 3 cut(s) 117, 302, 355
Hin1II CATG 1 cut(s) 387
HinfI GANTC 2 cut(s) 158, 260
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 2 cut(s) 6, 85
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 111, 122, 359
Hpy8I GTNNAC 2 cut(s) 6, 85
Hpy99I CGWCG 2 cut(s) 155, 175
HpyAV CCTTC 2 cut(s) 191, 217
HpyCH4III ACNGT 2 cut(s) 175, 211
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 7 cut(s) 85, 218, 341, 351, 367, 387, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 1 cut(s) 387
Kzo9I GATC 3 cut(s) 23, 40, 124
LpnPI CCDG 4 cut(s) 242, 288, 337, 373
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 2 cut(s) 45, 116
MaeI CTAG 2 cut(s) 80, 448
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 137, 200, 269
MalI GATC 3 cut(s) 25, 42, 126
MboI GATC 3 cut(s) 23, 40, 124
MboII GAAGA 7 cut(s) 25, 40, 167, 308, 320, 406, 409
MhlI GDGCHC 2 cut(s) 87, 337
MluCI AATT 4 cut(s) 278, 342, 362, 424
MlyI GAGTC 1 cut(s) 269
MseI TTAA 2 cut(s) 252, 423
MwoI GCNNNNNNNGC 1 cut(s) 64
NdeII GATC 3 cut(s) 23, 40, 124
NlaIII CATG 1 cut(s) 387
NlaIV GGNNCC 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 137, 200
NspI RCATGY 1 cut(s) 387
PceI AGGCCT 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 1 cut(s) 66
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 116
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 2 cut(s) 252, 423
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 3 cut(s) 23, 40, 124
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 87, 337
SetI ASST 7 cut(s) 49, 81, 192, 202, 245, 290, 334
SfaNI GCATC 2 cut(s) 45, 116
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 4 cut(s) 278, 342, 362, 424
SseBI AGGCCT 1 cut(s) 355
SsiI CCGC 1 cut(s) 21
SspI AATATT 1 cut(s) 421
SspMI CTAG 2 cut(s) 80, 448
StuI AGGCCT 1 cut(s) 355
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 175, 211
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 26, 43, 150
TasI AATT 4 cut(s) 278, 342, 362, 424
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 252, 423
Tru9I TTAA 2 cut(s) 252, 423
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 2 cut(s) 137, 200
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 2 cut(s) 137, 200
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 2 cut(s) 23, 330
TspRI CASTG 1 cut(s) 225
VneI GTGCAC 1 cut(s) 83
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 278
XceI RCATGY 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 80, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.