MD04G1102600.v1.1

Protamine P1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013294
Physical Location & Seq
Reverse (-)
18995855 .. 18996832
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1102600.v1.1.491

Sequence Viewer

Length: 978 bp
ATGAAGCTATCTGCAAAACCCATATCGAGTCCGGGGCGGACGGACAAGTTTCCGCCGCCGTTGGTGAGGTTTCTGAGGACCAACGTCGGGAGAAGAAGCAGAGGCCGGTTGCGTTCGAGCCCAATGTTCGTGAGGAAGAAGAACGCCGCCGCCGCCGCTGTCGAAACCCAAGAGCCGTCTTCTCCTAAAGTCACGTGCATGGGGCAAGTCCGAGTCCGGCGGTCATCTAAGCAAGGCGGTTCGCGGACCGGAGGAGCCAGACGGGCCACCGGAGCTCCGACCCGACGAAGGTGCAAATGGATCTCAAACGCCCTGTTTTGCCGCCATTTTTCCGGGATAATCAAGGCCAAGTCTTTTCGACCCGTTTGGCGCAAATGGGTACCGTTTTTTCAACTGGGTTTTTGTAGAAAAGGCGAAATCAAAGAGGACCCACCAAAGATTGAATCGAAATTTGAAGACCCAATTGAAGGTTGTTCAGATGAGGAAGATTTTATCGAAGAACAAGAAGACCAAGAATTCGAAAGAAAGGCCAAAGCTTTCGTCTATTCTTCTTCTGCCTCCACGCCGCCGAAGAACGCTTTGCTGTTAACCCGATGCAGATCTGCGCCGTACAGGACGTCGTCTCTGGCGAGCAGATTTTGGGGGTCGCCGGTGAGAGCCGAGGAAACAGAGGCACAACAAAAACCTACGTCAGAAGGGGAGTCGATTTCGGGTCAGGAATCGAGATTGGAATCGGAGAGCGAAGAAAAGTTGGAATTTTTCAGGGAATTGGAGAGCGAAATTCGAGAGAGAATGGCGAAATCGGCGAGCACCGAAGATAAAGACTGGGATTTCGTCGGGTCGGGTCGGCCGCTGATACTGACGAGGTGTAAATCGGAGCCGGCGAGAACGGCGGCGGAGAAGCTCGACCCGGAGTTGTGTTTCTGGAAGACGAGAAGGTTGGGAATAGTTGACTCATGCTCACCCAGTATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

326

Amino Acids

36.77

Weight (kDa)

9.72

Isoelectric Point (pI)

71.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37100 AT5G03110
fragaria_vesca FvH4_6g15510
malus_domestica MD04G1102600.v1.1 MD12G1122400.v1.1
prunus_persica Prupe.6G240400_v2.0.a1
pyrus_communis pycom12g12120
rosa_chinensis RchiOBHm_Chr3g0469011
rosa_laevigata RLG00000024390
rosa_multiflora Rmu_co8173580.1_g000001 Rmu_sc0037667.1_g000001
rosa_roxburghii Rroxscaffold_2G00112190 Rroxscaffold_6G00412370
rosa_rugosa Rorug03G0098400
rosa_samantha Rh3AG147900 Rh3BG170500 Rh3DG204500
rosa_wichuraiana Rw3G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 620
Acc65I GGTACC 1 cut(s) 379
AccB1I GGYRCC 1 cut(s) 379
AccII CGCG 1 cut(s) 244
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 848
AcsI RAATTY 4 cut(s) 449, 515, 755, 780
AcvI CACGTG 1 cut(s) 195
AcyI GRCGYC 1 cut(s) 617
AfaI GTAC 2 cut(s) 381, 611
AfiI CCNNNNNNNGG 3 cut(s) 87, 288, 467
AgsI TTSAA 4 cut(s) 392, 443, 455, 467
AluBI AGCT 4 cut(s) 7, 275, 536, 904
AluI AGCT 4 cut(s) 7, 275, 536, 904
Alw21I GWGCWC 2 cut(s) 277, 812
Alw26I GTCTC 1 cut(s) 627
AlwI GGATC 1 cut(s) 308
AoxI GGCC 5 cut(s) 103, 264, 345, 528, 848
ApoI RAATTY 4 cut(s) 449, 515, 755, 780
ArsI GACNNNNNNTTYG 6 cut(s) 500, 525, 532, 557, 815, 847
Asp718I GGTACC 1 cut(s) 379
AspLEI GCGC 2 cut(s) 372, 607
AspS9I GGNCC 4 cut(s) 78, 246, 264, 427
AsuC2I CCSGG 3 cut(s) 33, 334, 911
AsuHPI GGTGA 3 cut(s) 76, 664, 954
AsuII TTCGAA 1 cut(s) 519
AvaII GGWCC 3 cut(s) 78, 246, 427
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 2 cut(s) 122, 277
BbrPI CACGTG 1 cut(s) 195
BbsI GAAGAC 4 cut(s) 171, 462, 513, 935
Bbv12I GWGCWC 2 cut(s) 277, 812
BceAI ACGGC 4 cut(s) 43, 160, 592, 906
BcgI CGANNNNNNTGC 2 cut(s) 273, 307
BcnI CCSGG 3 cut(s) 33, 334, 911
BcoDI GTCTC 1 cut(s) 627
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 1 cut(s) 599
BisI GCNGC 9 cut(s) 56, 147, 150, 153, 156, 322, 566, 851, 894
BlsI GCNGC 9 cut(s) 57, 148, 151, 154, 157, 323, 567, 852, 895
Bme1390I CCNGG 3 cut(s) 33, 334, 911
Bme18I GGWCC 3 cut(s) 78, 246, 427
BmgT120I GGNCC 4 cut(s) 78, 246, 264, 427
BmiI GGNNCC 4 cut(s) 256, 381, 429, 879
BmrFI CCNGG 3 cut(s) 33, 334, 911
BmrI ACTGGG 3 cut(s) 404, 835, 960
BmsI GCATC 1 cut(s) 584
BmuI ACTGGG 3 cut(s) 404, 835, 960
BoxI GACNNNNGTC 1 cut(s) 83
BpiI GAAGAC 4 cut(s) 171, 462, 513, 935
Bpu14I TTCGAA 1 cut(s) 519
BpuMI CCSGG 3 cut(s) 33, 334, 911
BsaAI YACGTR 1 cut(s) 195
BsaBI GATNNNNATC 2 cut(s) 598, 730
BsaHI GRCGYC 1 cut(s) 617
BsaJI CCNNGG 2 cut(s) 32, 660
BsaWI WCCGGW 2 cut(s) 248, 269
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 288, 467
Bse118I RCCGGY 3 cut(s) 105, 649, 880
Bse1I ACTGG 3 cut(s) 399, 830, 966
Bse8I GATNNNNATC 2 cut(s) 598, 730
BseDI CCNNGG 2 cut(s) 32, 660
BseJI GATNNNNATC 2 cut(s) 598, 730
BseLI CCNNNNNNNGG 3 cut(s) 87, 288, 467
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 3 cut(s) 399, 830, 966
BseRI GAGGAG 1 cut(s) 267
BseX3I CGGCCG 1 cut(s) 848
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 851
BshFI GGCC 5 cut(s) 105, 266, 347, 530, 850
BshNI GGYRCC 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 851
BsiHKAI GWGCWC 2 cut(s) 277, 812
BsiSI CCGG 9 cut(s) 32, 106, 217, 249, 270, 333, 650, 881, 911
BslI CCNNNNNNNGG 3 cut(s) 87, 288, 467
BsmAI GTCTC 1 cut(s) 627
BsmBI CGTCTC 1 cut(s) 627
BsnI GGCC 5 cut(s) 105, 266, 347, 530, 850
Bsp119I TTCGAA 1 cut(s) 519
Bsp1286I GDGCHC 3 cut(s) 122, 277, 812
Bsp143I GATC 2 cut(s) 300, 599
BspANI GGCC 5 cut(s) 105, 266, 347, 530, 850
BspCNI CTCAG 1 cut(s) 66
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 4 cut(s) 256, 381, 429, 879
BspPI GGATC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 379
BsrFI RCCGGY 3 cut(s) 105, 649, 880
BsrI ACTGG 3 cut(s) 399, 830, 966
BssAI RCCGGY 3 cut(s) 105, 649, 880
BssECI CCNNGG 2 cut(s) 32, 660
BssMI GATC 2 cut(s) 300, 599
BssNI GRCGYC 1 cut(s) 617
Bst4CI ACNGT 1 cut(s) 384
BstACI GRCGYC 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 195
BstBI TTCGAA 1 cut(s) 519
BstC8I GCNNGC 3 cut(s) 631, 808, 882
BstDEI CTNAG 2 cut(s) 74, 228
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 2 cut(s) 372, 607
BstKTI GATC 2 cut(s) 303, 602
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 2 cut(s) 300, 599
BstMCI CGRYCG 1 cut(s) 851
BstMWI GCNNNNNNNGC 6 cut(s) 152, 155, 263, 272, 803, 890
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 3 cut(s) 31, 332, 909
BstUI CGCG 1 cut(s) 244
BstV2I GAAGAC 4 cut(s) 171, 462, 513, 935
BstX2I RGATCY 2 cut(s) 300, 599
BstYI RGATCY 2 cut(s) 300, 599
BstZI CGGCCG 1 cut(s) 848
BsuRI GGCC 5 cut(s) 105, 266, 347, 530, 850
Cac8I GCNNGC 3 cut(s) 631, 808, 882
CfoI GCGC 2 cut(s) 372, 607
Cfr10I RCCGGY 3 cut(s) 105, 649, 880
Cfr13I GGNCC 4 cut(s) 78, 246, 264, 427
CpoI CGGWCCG 1 cut(s) 246
Csp6I GTAC 2 cut(s) 380, 610
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 2 cut(s) 199, 957
CviQI GTAC 2 cut(s) 380, 610
DdeI CTNAG 2 cut(s) 74, 228
DpnI GATC 2 cut(s) 302, 601
DpnII GATC 2 cut(s) 300, 599
EaeI YGGCCR 1 cut(s) 848
EagI CGGCCG 1 cut(s) 848
EciI GGCGGA 3 cut(s) 42, 52, 911
Ecl136II GAGCTC 1 cut(s) 275
EclXI CGGCCG 1 cut(s) 848
Eco24I GRGCYC 2 cut(s) 122, 277
Eco47I GGWCC 3 cut(s) 78, 246, 427
Eco52I CGGCCG 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 275
Eco72I CACGTG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 515
EcoT38I GRGCYC 2 cut(s) 122, 277
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 2 cut(s) 202, 960
FaiI YATR 3 cut(s) 23, 200, 958
FatI CATG 2 cut(s) 198, 956
Fnu4HI GCNGC 9 cut(s) 56, 147, 150, 153, 156, 322, 566, 851, 894
FriOI GRGCYC 2 cut(s) 122, 277
Fsp4HI GCNGC 9 cut(s) 56, 147, 150, 153, 156, 322, 566, 851, 894
GlaI GCGC 2 cut(s) 371, 606
GluI GCNGC 9 cut(s) 56, 147, 150, 153, 156, 322, 566, 851, 894
HaeIII GGCC 5 cut(s) 105, 266, 347, 530, 850
HapII CCGG 9 cut(s) 32, 106, 217, 249, 270, 333, 650, 881, 911
HhaI GCGC 2 cut(s) 372, 607
Hin1I GRCGYC 1 cut(s) 617
Hin1II CATG 2 cut(s) 202, 960
Hin6I GCGC 2 cut(s) 370, 605
HinP1I GCGC 2 cut(s) 370, 605
HincII GTYRAC 2 cut(s) 588, 952
HindII GTYRAC 2 cut(s) 588, 952
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 7 cut(s) 28, 213, 443, 701, 719, 731, 953
HpaI GTTAAC 1 cut(s) 588
HpaII CCGG 9 cut(s) 32, 106, 217, 249, 270, 333, 650, 881, 911
HphI GGTGA 3 cut(s) 76, 664, 954
Hpy166II GTNNAC 2 cut(s) 588, 952
Hpy188I TCNGA 7 cut(s) 75, 212, 279, 478, 694, 736, 877
Hpy188III TCNNGA 6 cut(s) 88, 130, 716, 723, 785, 925
Hpy8I GTNNAC 2 cut(s) 588, 952
Hpy99I CGWCG 4 cut(s) 89, 288, 622, 839
HpyAV CCTTC 4 cut(s) 282, 461, 689, 930
HpyCH4III ACNGT 1 cut(s) 384
HpyCH4IV ACGT 4 cut(s) 84, 194, 617, 689
HpyCH4V TGCA 4 cut(s) 14, 198, 294, 597
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 155, 263, 272, 803, 890
HpyF3I CTNAG 2 cut(s) 74, 228
HpySE526I ACGT 4 cut(s) 84, 194, 617, 689
Hsp92I GRCGYC 1 cut(s) 617
Hsp92II CATG 2 cut(s) 202, 960
HspAI GCGC 2 cut(s) 370, 605
KpnI GGTACC 1 cut(s) 383
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
KspAI GTTAAC 1 cut(s) 588
Kzo9I GATC 2 cut(s) 300, 599
LmnI GCTCC 4 cut(s) 254, 272, 280, 877
LweI GCATC 1 cut(s) 584
MaeII ACGT 4 cut(s) 84, 194, 617, 689
MaeIII GTNAC 1 cut(s) 190
MalI GATC 2 cut(s) 302, 601
MboI GATC 2 cut(s) 300, 599
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 300, 599
MhlI GDGCHC 3 cut(s) 122, 277, 812
MluCI AATT 6 cut(s) 449, 462, 515, 755, 767, 780
MlyI GAGTC 4 cut(s) 37, 222, 710, 947
MmeI TCCRAC 2 cut(s) 302, 732
MroNI GCCGGC 1 cut(s) 880
MseI TTAA 1 cut(s) 587
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 2 cut(s) 158, 853
MspI CCGG 9 cut(s) 32, 106, 217, 249, 270, 333, 650, 881, 911
MspR9I CCNGG 3 cut(s) 33, 334, 911
MunI CAATTG 1 cut(s) 462
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 6 cut(s) 152, 155, 263, 272, 803, 890
NaeI GCCGGC 1 cut(s) 882
NciI CCSGG 3 cut(s) 33, 334, 911
NdeII GATC 2 cut(s) 300, 599
NgoMIV GCCGGC 1 cut(s) 880
NlaIII CATG 2 cut(s) 202, 960
NlaIV GGNNCC 4 cut(s) 256, 381, 429, 879
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 190
NspV TTCGAA 1 cut(s) 519
PcsI WCGNNNNNNNCGW 2 cut(s) 626, 881
PdiI GCCGGC 1 cut(s) 882
PfeI GAWTC 3 cut(s) 443, 719, 731
PflFI GACNNNGTC 1 cut(s) 619
PfoI TCCNGGA 1 cut(s) 332
PkrI GCNGC 9 cut(s) 57, 148, 151, 154, 157, 323, 567, 852, 895
PleI GAGTC 4 cut(s) 36, 221, 709, 947
PmaCI CACGTG 1 cut(s) 195
PmlI CACGTG 1 cut(s) 195
PpsI GAGTC 4 cut(s) 36, 221, 709, 947
Ppu21I YACGTR 1 cut(s) 195
PpuMI RGGWCCY 1 cut(s) 427
PshAI GACNNNNGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 277
Psp5II RGGWCCY 1 cut(s) 427
PspCI CACGTG 1 cut(s) 195
PspN4I GGNNCC 4 cut(s) 256, 381, 429, 879
PspPI GGNCC 4 cut(s) 78, 246, 264, 427
PspPPI RGGWCCY 1 cut(s) 427
PsuI RGATCY 2 cut(s) 300, 599
PsyI GACNNNGTC 1 cut(s) 619
RsaI GTAC 2 cut(s) 381, 611
RsaNI GTAC 2 cut(s) 380, 610
RseI CAYNNNNRTG 1 cut(s) 197
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 9 cut(s) 56, 147, 150, 153, 156, 322, 566, 851, 894
Sau3AI GATC 2 cut(s) 300, 599
Sau96I GGNCC 4 cut(s) 78, 246, 264, 427
SchI GAGTC 4 cut(s) 37, 222, 710, 947
ScrFI CCNGG 3 cut(s) 33, 334, 911
SduI GDGCHC 3 cut(s) 122, 277, 812
SfaNI GCATC 1 cut(s) 584
SfuI TTCGAA 1 cut(s) 519
SgrAI CRCCGGYG 1 cut(s) 649
SinI GGWCC 3 cut(s) 78, 246, 427
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 6 cut(s) 449, 462, 515, 755, 767, 780
SstI GAGCTC 1 cut(s) 277
StyD4I CCNGG 3 cut(s) 31, 332, 909
TaaI ACNGT 1 cut(s) 384
TaiI ACGT 4 cut(s) 87, 197, 620, 692
TasI AATT 6 cut(s) 449, 462, 515, 755, 767, 780
TauI GCSGC 9 cut(s) 58, 149, 152, 155, 158, 324, 568, 853, 896
TfiI GAWTC 3 cut(s) 443, 719, 731
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 56
Tth111I GACNNNGTC 1 cut(s) 619
VpaK11BI GGWCC 3 cut(s) 78, 246, 427
XapI RAATTY 4 cut(s) 449, 515, 755, 780
ZraI GACGTC 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.