MD12G1122400.v1.1

Protamine P1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013294
Physical Location & Seq
Reverse (-)
19706180 .. 19707151
972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1122400.v1.1.491

Sequence Viewer

Length: 972 bp
ATGAAGCTCTCTGCAAAACCCATGTCGAGTCCGGGTCGGACGGACAAGTTCCCGCCGCCGTTGGTGAGGTTTCTGAGGACCAACGTCGGGAGCAGAAGCAGAGCCCGGGTGCGTTCGAGCCCAATGTTCGTGAGGAAGAAGAACACACCCGCCGTTGAAACACAAGAGCCGTCGTCTCCTAAAGTCACGTGCATGGGGCAAGTCCGAGTCCGGAGGTCCTCCAAGCAAGGCGGTTCGCGGCCCCGGCGAGCTAGAGGAACTACCGGAGCTCCTTCCCGACGGCGATGCAAATGGATCCGAACCGCCCTGTTTTCCCGCCATTTTCCCGGGAAAATCAAGGCCAAGTCTTTTCGACCCGTCTGGCGCAAATGGGTACCTTTTTTCCAACTGGGTTTTTGCAGAAAGGGAGAAGTCAAAAAGGACCCACCAAAGATTGAATCGAAATTTGAAGACCCAATTGAAGGTCGTTCAGATGGGGAAGATTTTGGCGAAGAACAAGGAGACCAAGAATTCGAAAGAAAGGAGAAAGCTTTCGTCGTTTCTCCTTCTTTGTCCACGCCGCCGAGGAACGCCTTGCTGTTAACCCGATGCAGATCTGCGCCTCACAGGTCATCGTCTCTGGCGAGCAGATTTTGGGGTTCGCCGGTGAGAGCCGAGGAAACAGAGGAAGAACAGAAACCCACGTCGGAAAGGCAGTCGATTTCGGATGAGGAAGCGAGATTGGAGTCGGAGAGCGAAGAAAAGTTGGAATTTTTCAGGGAATTGGAGGGCGAAATTCGAGAAAGAATGGCGAAATCGGCGAGCATCGAAGATAAAGACGGAGACTTCGGCGGGTCGGCTCGGCCGCTGATACTGACGAGGTGTAAATCGGAACCGGCGAGAACCGCGGCGGAGAAGCTCGACCCGGAGTTGGGTTTCTGGAAAACGAGAAGGTTGGGAATTGTTGATTCATGCTCACCGAGTGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

324

Amino Acids

36.64

Weight (kDa)

10.13

Isoelectric Point (pI)

75.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37100 AT5G03110
fragaria_vesca FvH4_6g15510
malus_domestica MD04G1102600.v1.1 MD12G1122400.v1.1
prunus_persica Prupe.6G240400_v2.0.a1
pyrus_communis pycom12g12120
rosa_chinensis RchiOBHm_Chr3g0469011
rosa_laevigata RLG00000024390
rosa_multiflora Rmu_co8173580.1_g000001 Rmu_sc0037667.1_g000001
rosa_roxburghii Rroxscaffold_2G00112190 Rroxscaffold_6G00412370
rosa_rugosa Rorug03G0098400
rosa_samantha Rh3AG147900 Rh3BG170500 Rh3DG204500
rosa_wichuraiana Rw3G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 373
AccB1I GGYRCC 1 cut(s) 373
AccII CGCG 2 cut(s) 238, 887
AccIII TCCGGA 1 cut(s) 210
AclWI GGATC 2 cut(s) 289, 302
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 4 cut(s) 443, 509, 749, 774
AcvI CACGTG 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 962
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 4 cut(s) 87, 461, 910, 911
AgsI TTSAA 4 cut(s) 158, 437, 449, 461
AjiI CACGTC 1 cut(s) 684
AluBI AGCT 5 cut(s) 7, 251, 269, 530, 898
AluI AGCT 5 cut(s) 7, 251, 269, 530, 898
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 4 cut(s) 180, 495, 621, 816
AlwI GGATC 2 cut(s) 289, 302
Ama87I CYCGRG 2 cut(s) 105, 326
Aor13HI TCCGGA 1 cut(s) 210
AoxI GGCC 3 cut(s) 239, 339, 842
ApoI RAATTY 4 cut(s) 443, 509, 749, 774
ArsI GACNNNNNNTTYG 6 cut(s) 8, 40, 494, 526, 809, 841
Asp700I GAANNNNTTC 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 373
AspLEI GCGC 2 cut(s) 366, 601
AspS9I GGNCC 4 cut(s) 78, 216, 240, 421
AsuC2I CCSGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
AsuHPI GGTGA 3 cut(s) 76, 658, 948
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 2 cut(s) 105, 326
AvaII GGWCC 3 cut(s) 78, 216, 421
BamHI GGATCC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 3 cut(s) 106, 122, 271
BbrPI CACGTG 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 271
BccI CCATC 1 cut(s) 467
BceAI ACGGC 4 cut(s) 43, 137, 154, 296
BcgI CGANNNNNNTGC 2 cut(s) 267, 301
BcnI CCSGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
BcoDI GTCTC 4 cut(s) 180, 495, 621, 816
BfaI CTAG 1 cut(s) 252
BglII AGATCT 1 cut(s) 593
BisI GCNGC 5 cut(s) 56, 239, 560, 845, 888
BlsI GCNGC 5 cut(s) 57, 240, 561, 846, 889
Bme1390I CCNGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
Bme18I GGWCC 3 cut(s) 78, 216, 421
BmeT110I CYCGRG 2 cut(s) 105, 326
BmgBI CACGTC 1 cut(s) 684
BmgT120I GGNCC 4 cut(s) 78, 216, 240, 421
BmiI GGNNCC 5 cut(s) 242, 296, 375, 423, 873
BmrFI CCNGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
BmrI ACTGGG 1 cut(s) 398
BmsI GCATC 3 cut(s) 275, 578, 813
BmuI ACTGGG 1 cut(s) 398
BoxI GACNNNNGTC 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 513
BpuMI CCSGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
BsaAI YACGTR 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 592
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 6 cut(s) 105, 242, 326, 563, 654, 885
BsaWI WCCGGW 2 cut(s) 210, 263
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 461, 910, 911
Bse118I RCCGGY 2 cut(s) 643, 874
Bse1I ACTGG 1 cut(s) 393
Bse8I GATNNNNATC 1 cut(s) 592
BseAI TCCGGA 1 cut(s) 210
BseDI CCNNGG 6 cut(s) 105, 242, 326, 563, 654, 885
BseGI GGATG 1 cut(s) 712
BseJI GATNNNNATC 1 cut(s) 592
BseLI CCNNNNNNNGG 4 cut(s) 87, 461, 910, 911
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 1 cut(s) 393
BseX3I CGGCCG 1 cut(s) 842
Bsh1236I CGCG 2 cut(s) 238, 887
Bsh1285I CGRYCG 1 cut(s) 845
BshFI GGCC 3 cut(s) 241, 341, 844
BshNI GGYRCC 1 cut(s) 373
BsiEI CGRYCG 1 cut(s) 845
BsiHKAI GWGCWC 1 cut(s) 271
BsiHKCI CYCGRG 2 cut(s) 105, 326
BsiSI CCGG 9 cut(s) 32, 106, 211, 244, 264, 327, 644, 875, 905
BslI CCNNNNNNNGG 4 cut(s) 87, 461, 910, 911
BsmAI GTCTC 4 cut(s) 180, 495, 621, 816
BsmBI CGTCTC 2 cut(s) 180, 621
BsnI GGCC 3 cut(s) 241, 341, 844
Bso31I GGTCTC 1 cut(s) 495
BsoBI CYCGRG 2 cut(s) 105, 326
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 3 cut(s) 106, 122, 271
Bsp13I TCCGGA 1 cut(s) 210
Bsp143I GATC 2 cut(s) 294, 593
BspANI GGCC 3 cut(s) 241, 341, 844
BspCNI CTCAG 1 cut(s) 66
BspEI TCCGGA 1 cut(s) 210
BspFNI CGCG 2 cut(s) 238, 887
BspLI GGNNCC 5 cut(s) 242, 296, 375, 423, 873
BspPI GGATC 2 cut(s) 289, 302
BspT104I TTCGAA 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 495
BsrFI RCCGGY 2 cut(s) 643, 874
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 2 cut(s) 643, 874
BssECI CCNNGG 6 cut(s) 105, 242, 326, 563, 654, 885
BssMI GATC 2 cut(s) 294, 593
BstBAI YACGTR 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 3 cut(s) 249, 625, 802
BstDEI CTNAG 1 cut(s) 74
BstDSI CCRYGG 1 cut(s) 885
BstF5I GGATG 1 cut(s) 712
BstFNI CGCG 2 cut(s) 238, 887
BstHHI GCGC 2 cut(s) 366, 601
BstKTI GATC 2 cut(s) 297, 596
BstMAI GTCTC 4 cut(s) 180, 495, 621, 816
BstMBI GATC 2 cut(s) 294, 593
BstMCI CGRYCG 1 cut(s) 845
BstMWI GCNNNNNNNGC 3 cut(s) 244, 797, 884
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 7 cut(s) 31, 104, 105, 242, 325, 326, 903
BstUI CGCG 2 cut(s) 238, 887
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 2 cut(s) 294, 593
BstYI RGATCY 2 cut(s) 294, 593
BstZI CGGCCG 1 cut(s) 842
BsuRI GGCC 3 cut(s) 241, 341, 844
BtgI CCRYGG 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 684
BtsCI GGATG 1 cut(s) 712
Cac8I GCNNGC 3 cut(s) 249, 625, 802
CfoI GCGC 2 cut(s) 366, 601
Cfr10I RCCGGY 2 cut(s) 643, 874
Cfr13I GGNCC 4 cut(s) 78, 216, 240, 421
Cfr42I CCGCGG 1 cut(s) 888
Cfr9I CCCGGG 2 cut(s) 105, 326
Csp6I GTAC 1 cut(s) 374
CviAII CATG 3 cut(s) 22, 193, 951
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 1 cut(s) 74
DpnI GATC 2 cut(s) 296, 595
DpnII GATC 2 cut(s) 294, 593
DraIII CACNNNGTG 1 cut(s) 962
EaeI YGGCCR 1 cut(s) 842
EagI CGGCCG 1 cut(s) 842
EciI GGCGGA 1 cut(s) 905
Ecl136II GAGCTC 1 cut(s) 269
EclXI CGGCCG 1 cut(s) 842
Eco24I GRGCYC 3 cut(s) 106, 122, 271
Eco31I GGTCTC 1 cut(s) 495
Eco47I GGWCC 3 cut(s) 78, 216, 421
Eco52I CGGCCG 1 cut(s) 842
Eco53kI GAGCTC 1 cut(s) 269
Eco72I CACGTG 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 105, 326
EcoICRI GAGCTC 1 cut(s) 269
EcoO109I RGGNCCY 2 cut(s) 216, 421
EcoRI GAATTC 1 cut(s) 509
EcoT38I GRGCYC 3 cut(s) 106, 122, 271
Esp3I CGTCTC 2 cut(s) 180, 621
FaeI CATG 3 cut(s) 25, 196, 954
FaiI YATR 3 cut(s) 23, 194, 952
FatI CATG 3 cut(s) 21, 192, 950
FauI CCCGC 4 cut(s) 60, 157, 323, 824
Fnu4HI GCNGC 5 cut(s) 56, 239, 560, 845, 888
FokI GGATG 1 cut(s) 719
FriOI GRGCYC 3 cut(s) 106, 122, 271
Fsp4HI GCNGC 5 cut(s) 56, 239, 560, 845, 888
FspBI CTAG 1 cut(s) 252
GlaI GCGC 2 cut(s) 365, 600
GluI GCNGC 5 cut(s) 56, 239, 560, 845, 888
HaeIII GGCC 3 cut(s) 241, 341, 844
HapII CCGG 9 cut(s) 32, 106, 211, 244, 264, 327, 644, 875, 905
HhaI GCGC 2 cut(s) 366, 601
Hin1II CATG 3 cut(s) 25, 196, 954
Hin6I GCGC 2 cut(s) 364, 599
HinP1I GCGC 2 cut(s) 364, 599
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 5 cut(s) 28, 207, 437, 725, 947
HpaI GTTAAC 1 cut(s) 582
HpaII CCGG 9 cut(s) 32, 106, 211, 244, 264, 327, 644, 875, 905
HphI GGTGA 3 cut(s) 76, 658, 948
Hpy166II GTNNAC 2 cut(s) 555, 582
Hpy188I TCNGA 9 cut(s) 39, 75, 206, 299, 472, 688, 706, 730, 871
Hpy188III TCNNGA 6 cut(s) 88, 130, 211, 276, 779, 919
Hpy8I GTNNAC 2 cut(s) 555, 582
Hpy99I CGWCG 5 cut(s) 89, 175, 282, 539, 688
HpyAV CCTTC 4 cut(s) 282, 455, 555, 924
HpyCH4IV ACGT 3 cut(s) 84, 188, 683
HpyCH4V TGCA 5 cut(s) 14, 192, 288, 399, 591
HpyF10VI GCNNNNNNNGC 3 cut(s) 244, 797, 884
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 3 cut(s) 84, 188, 683
Hsp92II CATG 3 cut(s) 25, 196, 954
HspAI GCGC 2 cut(s) 364, 599
Kpn2I TCCGGA 1 cut(s) 210
KpnI GGTACC 1 cut(s) 377
KspAI GTTAAC 1 cut(s) 582
KspI CCGCGG 1 cut(s) 888
Kzo9I GATC 2 cut(s) 294, 593
LmnI GCTCC 3 cut(s) 90, 266, 274
LweI GCATC 3 cut(s) 275, 578, 813
MaeI CTAG 1 cut(s) 252
MaeII ACGT 3 cut(s) 84, 188, 683
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 296, 595
MboI GATC 2 cut(s) 294, 593
MboII GAAGA 8 cut(s) 148, 151, 461, 491, 503, 680, 749, 821
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 2 cut(s) 294, 593
MhlI GDGCHC 3 cut(s) 106, 122, 271
MluCI AATT 7 cut(s) 443, 456, 509, 749, 761, 774, 939
MlyI GAGTC 3 cut(s) 37, 216, 734
MmeI TCCRAC 5 cut(s) 17, 409, 666, 708, 726
MroI TCCGGA 1 cut(s) 210
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 2 cut(s) 847, 887
MspI CCGG 9 cut(s) 32, 106, 211, 244, 264, 327, 644, 875, 905
MspR9I CCNGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
MunI CAATTG 1 cut(s) 456
MvnI CGCG 2 cut(s) 238, 887
MwoI GCNNNNNNNGC 3 cut(s) 244, 797, 884
NciI CCSGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
NdeII GATC 2 cut(s) 294, 593
NlaIII CATG 3 cut(s) 25, 196, 954
NlaIV GGNNCC 5 cut(s) 242, 296, 375, 423, 873
NmeAIII GCCGAG 3 cut(s) 588, 679, 820
NmuCI GTSAC 1 cut(s) 184
NspV TTCGAA 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 620, 875
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 437, 947
PkrI GCNGC 5 cut(s) 57, 240, 561, 846, 889
PleI GAGTC 3 cut(s) 36, 215, 733
PmaCI CACGTG 1 cut(s) 189
PmlI CACGTG 1 cut(s) 189
PpsI GAGTC 3 cut(s) 36, 215, 733
Ppu21I YACGTR 1 cut(s) 189
PpuMI RGGWCCY 2 cut(s) 216, 421
PshAI GACNNNNGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 271
Psp5II RGGWCCY 2 cut(s) 216, 421
PspCI CACGTG 1 cut(s) 189
PspN4I GGNNCC 5 cut(s) 242, 296, 375, 423, 873
PspPI GGNCC 4 cut(s) 78, 216, 240, 421
PspPPI RGGWCCY 2 cut(s) 216, 421
PsuI RGATCY 2 cut(s) 294, 593
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 191
SacI GAGCTC 1 cut(s) 271
SacII CCGCGG 1 cut(s) 888
SaqAI TTAA 1 cut(s) 581
SatI GCNGC 5 cut(s) 56, 239, 560, 845, 888
Sau3AI GATC 2 cut(s) 294, 593
Sau96I GGNCC 4 cut(s) 78, 216, 240, 421
SchI GAGTC 3 cut(s) 37, 216, 734
ScrFI CCNGG 7 cut(s) 33, 106, 107, 244, 327, 328, 905
SduI GDGCHC 3 cut(s) 106, 122, 271
SfaNI GCATC 3 cut(s) 275, 578, 813
Sfr303I CCGCGG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 513
SgrAI CRCCGGYG 1 cut(s) 643
SgrBI CCGCGG 1 cut(s) 888
SinI GGWCC 3 cut(s) 78, 216, 421
SmaI CCCGGG 2 cut(s) 107, 328
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 7 cut(s) 443, 456, 509, 749, 761, 774, 939
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 271
StyD4I CCNGG 7 cut(s) 31, 104, 105, 242, 325, 326, 903
TaiI ACGT 3 cut(s) 87, 191, 686
TaqI TCGA 9 cut(s) 26, 116, 352, 440, 513, 698, 778, 807, 900
TasI AATT 7 cut(s) 443, 456, 509, 749, 761, 774, 939
TauI GCSGC 5 cut(s) 58, 241, 562, 847, 890
TfiI GAWTC 2 cut(s) 437, 947
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TseFI GTSAC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 17, 939
TspGWI ACGGA 2 cut(s) 56, 834
TspMI CCCGGG 2 cut(s) 105, 326
VpaK11BI GGWCC 3 cut(s) 78, 216, 421
XapI RAATTY 4 cut(s) 443, 509, 749, 774
XmaI CCCGGG 2 cut(s) 105, 326
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.