MD04G1109800.v1.1

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013725
Physical Location & Seq
Forward (+)
19431988 .. 19434914
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1109800.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGTTCACCTCTTTGTGGCATTACCTGCTATTGAGGACAGAATTGATGTCAAATGCATTCATAACTGTCGAAGTTTGAGTCTACACAGTTCTGCGGATTCACTCAATTGCAACAATCTCTTCTGCAACACTTGCACCGTGAAATCGATGAAATCGGGTTCCATTTGTCCAGTTTATAAAATTCCATATCGACGCAGAGGCACTGGGAAGACTACTCTGTCTACGGATCATAATCAGTATCTGATTGGAGATGATGAGCACTGCTGGAGCAACAATGGTGTGTCAAACATTAAAGGTCGTTGCTATGCCAATGCCAAGTGCATTGATTTGTTAAAGGACAAGGAACCAGATATTTGGAATGCCATCAAATTTGGGACTGGATCTTGGTGGATCACTAGGAAGAGATGCAGCAACCGGAAGAGGAGTACTTTTTGCGACAGAGGCTTTACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000460 GO:0000462 GO:0000463 GO:0000466 GO:0000470 GO:0000793 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0006997 GO:0007000 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007417 GO:0008104 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009267 GO:0009405 GO:0009605 GO:0009987 GO:0009991 GO:0010001 GO:0010467 GO:0010468 GO:0010570 GO:0010876 GO:0016043 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0019915 GO:0022008 GO:0022402 GO:0022613 GO:0030154 GO:0030447 GO:0030490 GO:0030684 GO:0030686 GO:0030687 GO:0031016 GO:0031017 GO:0031323 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032101 GO:0032102 GO:0032104 GO:0032105 GO:0032107 GO:0032108 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034470 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0035272 GO:0035690 GO:0036170 GO:0040007 GO:0040008 GO:0042063 GO:0042221 GO:0042254 GO:0042273 GO:0042274 GO:0042493 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044182 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0044786 GO:0045926 GO:0046483 GO:0048513 GO:0048519 GO:0048583 GO:0048585 GO:0048709 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051235 GO:0051252 GO:0051641 GO:0051704 GO:0051716 GO:0051726 GO:0055123 GO:0060255 GO:0060258 GO:0065007 GO:0065008 GO:0070013 GO:0070545 GO:0070727 GO:0070887 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090069 GO:0090304 GO:0097159 GO:1900428 GO:1900429 GO:1900434 GO:1900435 GO:1901360 GO:1901363 GO:1901576 GO:1990904 GO:2000232
KEGG Pathways
Metabolic & Signaling
ko04130 ko04721 ko04911 ko05031 map04130 map04721 map04911 map05031
Pfam Domains
Protein Families
BRCT_2 Pescadillo_N

Protein Analysis

152

Amino Acids

17.18

Weight (kDa)

9.21

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 67 - 126 1.4e-23 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AasI GACNNNNNNGTC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 34
AccI GTMKAC 2 cut(s) 82, 221
AciI CCGC 1 cut(s) 95
AclWI GGATC 3 cut(s) 234, 388, 398
AcsI RAATTY 2 cut(s) 180, 368
AfaI GTAC 1 cut(s) 427
AfiI CCNNNNNNNGG 1 cut(s) 16
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 3 cut(s) 234, 388, 398
AlwNI CAGNNNCTG 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 408
ApoI RAATTY 2 cut(s) 180, 368
ArsI GACNNNNNNTTYG 2 cut(s) 64, 96
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 420
BccI CCATC 1 cut(s) 371
BfaI CTAG 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
BmcAI AGTACT 1 cut(s) 427
BmiI GGNNCC 2 cut(s) 160, 345
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 395
BmuI ACTGGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 414
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse1I ACTGG 3 cut(s) 170, 208, 382
Bse8I GATNNNNATC 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 16
BseNI ACTGG 3 cut(s) 170, 208, 382
BseRI GAGGAG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 420
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 415
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 2 cut(s) 57, 364
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 226, 380, 390
BspACI CCGC 1 cut(s) 95
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 160, 345
BspMI ACCTGC 1 cut(s) 34
BspPI GGATC 3 cut(s) 234, 388, 398
BsrI ACTGG 3 cut(s) 170, 208, 382
BssMI GATC 3 cut(s) 226, 380, 390
Bst4CI ACNGT 3 cut(s) 68, 89, 139
Bst6I CTCTTC 3 cut(s) 125, 395, 413
BstAPI GCANNNNNTGC 2 cut(s) 26, 132
BstKTI GATC 3 cut(s) 229, 383, 393
BstMBI GATC 3 cut(s) 226, 380, 390
BstMWI GCNNNNNNNGC 3 cut(s) 26, 132, 441
BstV1I GCAGC 1 cut(s) 420
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 380
BstXI CCANNNNNNTGG 1 cut(s) 354
BstYI RGATCY 1 cut(s) 380
Bsu15I ATCGAT 1 cut(s) 146
BsuTUI ATCGAT 1 cut(s) 146
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 201, 259
BveI ACCTGC 1 cut(s) 34
CaiI CAGNNNCTG 1 cut(s) 241
ClaI ATCGAT 1 cut(s) 146
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 426
CviJI RGCY 1 cut(s) 444
CviKI_1 RGCY 1 cut(s) 444
CviQI GTAC 1 cut(s) 426
DpnI GATC 3 cut(s) 228, 382, 392
DpnII GATC 3 cut(s) 226, 380, 390
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 125, 395, 413
EarI CTCTTC 3 cut(s) 125, 395, 413
EcoT22I ATGCAT 1 cut(s) 59
FaiI YATR 5 cut(s) 63, 177, 187, 231, 306
FaqI GGGAC 1 cut(s) 388
FblI GTMKAC 2 cut(s) 82, 221
Fnu4HI GCNGC 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 1 cut(s) 396
GluI GCNGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 286
HapII CCGG 1 cut(s) 415
HgaI GACGC 1 cut(s) 201
HinfI GANTC 2 cut(s) 79, 98
HpaII CCGG 1 cut(s) 415
Hpy166II GTNNAC 3 cut(s) 7, 83, 222
Hpy188I TCNGA 1 cut(s) 243
Hpy8I GTNNAC 3 cut(s) 7, 83, 222
Hpy99I CGWCG 1 cut(s) 195
HpyCH4III ACNGT 3 cut(s) 68, 89, 139
HpyCH4V TGCA 6 cut(s) 57, 111, 126, 135, 321, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 132, 441
Kzo9I GATC 3 cut(s) 226, 380, 390
LmnI GCTCC 1 cut(s) 267
LpnPI CCDG 7 cut(s) 39, 183, 189, 250, 360, 363, 428
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 396
MalI GATC 3 cut(s) 228, 382, 392
MboI GATC 3 cut(s) 226, 380, 390
MboII GAAGA 4 cut(s) 112, 220, 412, 430
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 4 cut(s) 42, 106, 180, 368
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 5 cut(s) 20, 28, 191, 414, 434
Mph1103I ATGCAT 1 cut(s) 59
MseI TTAA 2 cut(s) 291, 332
MspI CCGG 1 cut(s) 415
MunI CAATTG 1 cut(s) 106
Mva1269I GAATGC 2 cut(s) 57, 364
MwoI GCNNNNNNNGC 3 cut(s) 26, 132, 441
NdeII GATC 3 cut(s) 226, 380, 390
NlaIV GGNNCC 2 cut(s) 160, 345
NsiI ATGCAT 1 cut(s) 59
PctI GAATGC 2 cut(s) 57, 364
PfeI GAWTC 1 cut(s) 98
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PsiI TTATAA 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 160, 345
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 380
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 291, 332
SatI GCNGC 1 cut(s) 409
Sau3AI GATC 3 cut(s) 226, 380, 390
ScaI AGTACT 1 cut(s) 427
SchI GAGTC 1 cut(s) 88
SduI GDGCHC 1 cut(s) 261
SetI ASST 3 cut(s) 12, 28, 298
SfaNI GCATC 1 cut(s) 395
Sse9I AATT 4 cut(s) 42, 106, 180, 368
SsiI CCGC 1 cut(s) 95
SspMI CTAG 1 cut(s) 396
TaaI ACNGT 3 cut(s) 68, 89, 139
TaqI TCGA 3 cut(s) 70, 146, 190
TasI AATT 4 cut(s) 42, 106, 180, 368
TatI WGTACW 1 cut(s) 425
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 2 cut(s) 291, 332
Tru9I TTAA 2 cut(s) 291, 332
TscAI CASTG 2 cut(s) 208, 266
TseI GCWGC 1 cut(s) 408
TspDTI ATGAA 2 cut(s) 50, 164
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 2 cut(s) 208, 266
XapI RAATTY 2 cut(s) 180, 368
XmiI GTMKAC 2 cut(s) 82, 221
XspI CTAG 1 cut(s) 396
ZrmI AGTACT 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.