MD17G1274500.v1.1

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
Physical Location & Seq
Reverse (-)
33605546 .. 33612409
6864 bp
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UTR
Exon/CDS
Intron
MD17G1274500.v1.1.491

Sequence Viewer

Length: 426 bp
ATGCAGGCCGAGGTTAAGGGGCAAAAAATCACATGGTTGACCCCTCACCCTTTGCATTTGAGTCTACTGAGTTCTGCGGATTCACTCAATTGCAATCATCTCTTCTACAACACTTGCACCGTGAAGTCGATGAAATTGGGTTCCATTTGTCCAGTTTATAAAATTCCATATCGACGCAGAGGCACTGGGAAGACGACTCTGTCTACGGATCATAATCGGTATCTAATTGGAGATGATGAGCACTGCTGGGGCAACAATGGTGTGTCAAACATTGAAGGTGGTTGCTATGCCAAGTGCATTGATTTGTCAATGGACAAGGAACCAGATATTTGGAATGCTATCAAATTTGGGACTGTTTTGGAGAATGTCGTGTTCGATGACCATAATCGAGAAGTTGATTACTCAGAGAAATCTGTTACTGTTTAG

Protein Analysis

142

Amino Acids

15.91

Weight (kDa)

6.28

Isoelectric Point (pI)

29.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 61 - 140 3.1e-39 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 159
AasI GACNNNNNNGTC 1 cut(s) 199
AccI GTMKAC 2 cut(s) 64, 203
AciI CCGC 1 cut(s) 77
AclWI GGATC 1 cut(s) 216
AcsI RAATTY 2 cut(s) 162, 344
AgsI TTSAA 1 cut(s) 275
Alw21I GWGCWC 1 cut(s) 243
AlwI GGATC 1 cut(s) 216
AoxI GGCC 1 cut(s) 6
ApoI RAATTY 2 cut(s) 162, 344
AsuHPI GGTGA 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 142, 321
BmrI ACTGGG 1 cut(s) 195
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 152, 190
Bse8I GATNNNNATC 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 9
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 2 cut(s) 59, 417
BseNI ACTGG 2 cut(s) 152, 190
BseYI CCCAGC 1 cut(s) 246
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 243
BslFI GGGAC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 1 cut(s) 77
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 60, 416
BspLI GGNNCC 2 cut(s) 142, 321
BspPI GGATC 1 cut(s) 216
BsrI ACTGG 2 cut(s) 152, 190
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 1 cut(s) 208
Bst4CI ACNGT 3 cut(s) 121, 355, 421
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 68, 403
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstV2I GAAGAC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 330
BsuRI GGCC 1 cut(s) 8
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 183, 241
Cac8I GCNNGC 1 cut(s) 6
CseI GACGC 1 cut(s) 183
CviAII CATG 1 cut(s) 33
CviJI RGCY 1 cut(s) 8
CviKI_1 RGCY 1 cut(s) 8
DdeI CTNAG 2 cut(s) 68, 403
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
DrdI GACNNNNNNGTC 1 cut(s) 199
DseDI GACNNNNNNGTC 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
FaeI CATG 1 cut(s) 36
FaiI YATR 6 cut(s) 34, 159, 169, 213, 288, 384
FaqI GGGAC 1 cut(s) 364
FatI CATG 1 cut(s) 32
FblI GTMKAC 2 cut(s) 64, 203
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 1 cut(s) 183
Hin1II CATG 1 cut(s) 36
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 3 cut(s) 61, 80, 196
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 39, 65, 204
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 3 cut(s) 39, 65, 204
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 3 cut(s) 121, 355, 421
HpyCH4V TGCA 5 cut(s) 4, 55, 93, 117, 297
HpyF3I CTNAG 2 cut(s) 68, 403
Hsp92II CATG 1 cut(s) 36
Kzo9I GATC 1 cut(s) 208
LpnPI CCDG 4 cut(s) 165, 171, 232, 336
MaeIII GTNAC 1 cut(s) 415
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 2 cut(s) 94, 202
MfeI CAATTG 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 5 cut(s) 88, 134, 162, 225, 344
MlyI GAGTC 2 cut(s) 70, 190
MnlI CCTC 3 cut(s) 4, 54, 173
MseI TTAA 1 cut(s) 15
MunI CAATTG 1 cut(s) 88
Mva1269I GAATGC 1 cut(s) 340
NdeII GATC 1 cut(s) 208
NlaIII CATG 1 cut(s) 36
NlaIV GGNNCC 2 cut(s) 142, 321
NmeAIII GCCGAG 1 cut(s) 34
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 1 cut(s) 80
PflFI GACNNNGTC 1 cut(s) 199
PleI GAGTC 2 cut(s) 69, 190
PpsI GAGTC 2 cut(s) 69, 190
PsiI TTATAA 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 142, 321
PsyI GACNNNGTC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 15
Sau3AI GATC 1 cut(s) 208
SchI GAGTC 2 cut(s) 70, 190
SduI GDGCHC 1 cut(s) 243
SetI ASST 2 cut(s) 15, 280
Sse9I AATT 5 cut(s) 88, 134, 162, 225, 344
SsiI CCGC 1 cut(s) 77
TaaI ACNGT 3 cut(s) 121, 355, 421
TaqI TCGA 4 cut(s) 128, 172, 375, 388
TasI AATT 5 cut(s) 88, 134, 162, 225, 344
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 190, 248
TspDTI ATGAA 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 2 cut(s) 190, 248
Tth111I GACNNNGTC 1 cut(s) 199
XapI RAATTY 2 cut(s) 162, 344
XmiI GTMKAC 2 cut(s) 64, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.