MD10G1043800.v1.1

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013725
Physical Location & Seq
Reverse (-)
5895478 .. 5897496
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1043800.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGGTTTGATTTTTCCCCATCTGTTTTGCAGTTTGAGTCTACTCAGTTCTGCGGATTCACTCAATTGCAATAATCTCTTCTGCAACACTTGCACCGTGAAATCGATGAAATCGGGTTCCATTTGTCCAGTTTATAAAATTCCATATCGACGCAGAGGCACTGGGAAGACTACTCTGTCTACGGATCATAATCAGTATCTGATTGGAGATGATGAGCACTGCTGGAGCAACAATGGTGTGTCAAACATTAAAGGTGGTTGCTATGCCAATGCCAAGTGCATTGATTTGTTAAGGGACAAGGAACCAGATATTTGGAATGCTATCAAATTTGGGATTGGATCTTGGTGGATCACTAGGAAGAGATGCAGCAACCGGAAGAGGAGTACTTTTTTGCGACAGAGGCTTTACTCAATGAGTATGTAA

Protein Analysis

141

Amino Acids

15.94

Weight (kDa)

9.45

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 53 - 111 9.9e-25 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
AasI GACNNNNNNGTC 1 cut(s) 175
AccI GTMKAC 2 cut(s) 40, 179
AciI CCGC 1 cut(s) 53
AclWI GGATC 3 cut(s) 192, 346, 356
AcsI RAATTY 2 cut(s) 138, 326
AfaI GTAC 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 219
AlwI GGATC 3 cut(s) 192, 346, 356
AlwNI CAGNNNCTG 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 2 cut(s) 138, 326
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 378
BccI CCATC 1 cut(s) 27
BfaI CTAG 1 cut(s) 354
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
BmcAI AGTACT 1 cut(s) 385
BmiI GGNNCC 2 cut(s) 118, 303
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 244
Bsa29I ATCGAT 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 372
Bse1I ACTGG 2 cut(s) 128, 166
Bse8I GATNNNNATC 1 cut(s) 189
BseCI ATCGAT 1 cut(s) 104
BseJI GATNNNNATC 1 cut(s) 189
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 2 cut(s) 128, 166
BseRI GAGGAG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 378
BshVI ATCGAT 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 3 cut(s) 184, 338, 348
BspACI CCGC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 57
BspDI ATCGAT 1 cut(s) 104
BspLI GGNNCC 2 cut(s) 118, 303
BspPI GGATC 3 cut(s) 192, 346, 356
BsrI ACTGG 2 cut(s) 128, 166
BssMI GATC 3 cut(s) 184, 338, 348
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 3 cut(s) 83, 353, 371
BstAPI GCANNNNNTGC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 44
BstKTI GATC 3 cut(s) 187, 341, 351
BstMBI GATC 3 cut(s) 184, 338, 348
BstMWI GCNNNNNNNGC 2 cut(s) 90, 400
BstV1I GCAGC 1 cut(s) 378
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 312
BstYI RGATCY 1 cut(s) 338
Bsu15I ATCGAT 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 2 cut(s) 159, 217
CaiI CAGNNNCTG 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 104
CseI GACGC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 384
CviJI RGCY 1 cut(s) 403
CviKI_1 RGCY 1 cut(s) 403
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 1 cut(s) 44
DpnI GATC 3 cut(s) 186, 340, 350
DpnII GATC 3 cut(s) 184, 338, 348
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
Eam1104I CTCTTC 3 cut(s) 83, 353, 371
EarI CTCTTC 3 cut(s) 83, 353, 371
FaiI YATR 5 cut(s) 135, 145, 189, 264, 419
FaqI GGGAC 1 cut(s) 308
FblI GTMKAC 2 cut(s) 40, 179
Fnu4HI GCNGC 1 cut(s) 367
Fsp4HI GCNGC 1 cut(s) 367
FspBI CTAG 1 cut(s) 354
GluI GCNGC 1 cut(s) 367
GsuI CTGGAG 1 cut(s) 244
HapII CCGG 1 cut(s) 373
HgaI GACGC 1 cut(s) 159
HinfI GANTC 2 cut(s) 37, 56
HpaII CCGG 1 cut(s) 373
Hpy166II GTNNAC 2 cut(s) 41, 180
Hpy188I TCNGA 1 cut(s) 201
Hpy8I GTNNAC 2 cut(s) 41, 180
Hpy99I CGWCG 1 cut(s) 153
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 6 cut(s) 30, 69, 84, 93, 279, 366
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 400
HpyF3I CTNAG 1 cut(s) 44
Kzo9I GATC 3 cut(s) 184, 338, 348
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 5 cut(s) 141, 147, 208, 318, 386
Lsp1109I GCAGC 1 cut(s) 378
LweI GCATC 1 cut(s) 353
MaeI CTAG 1 cut(s) 354
MalI GATC 3 cut(s) 186, 340, 350
MboI GATC 3 cut(s) 184, 338, 348
MboII GAAGA 4 cut(s) 70, 178, 370, 388
MfeI CAATTG 1 cut(s) 64
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 3 cut(s) 64, 138, 326
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 3 cut(s) 149, 372, 393
MseI TTAA 2 cut(s) 249, 290
MspI CCGG 1 cut(s) 373
MunI CAATTG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 90, 400
NdeII GATC 3 cut(s) 184, 338, 348
NlaIV GGNNCC 2 cut(s) 118, 303
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 1 cut(s) 368
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
PsiI TTATAA 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 118, 303
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SaqAI TTAA 2 cut(s) 249, 290
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 3 cut(s) 184, 338, 348
ScaI AGTACT 1 cut(s) 385
SchI GAGTC 1 cut(s) 46
SduI GDGCHC 1 cut(s) 219
SetI ASST 1 cut(s) 256
SfaNI GCATC 1 cut(s) 353
Sse9I AATT 3 cut(s) 64, 138, 326
SsiI CCGC 1 cut(s) 53
SspMI CTAG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 2 cut(s) 104, 148
TasI AATT 3 cut(s) 64, 138, 326
TatI WGTACW 1 cut(s) 383
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 2 cut(s) 249, 290
Tru9I TTAA 2 cut(s) 249, 290
TscAI CASTG 2 cut(s) 166, 224
TseI GCWGC 1 cut(s) 366
TspDTI ATGAA 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 166, 224
XapI RAATTY 2 cut(s) 138, 326
XmiI GTMKAC 2 cut(s) 40, 179
XspI CTAG 1 cut(s) 354
ZrmI AGTACT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.