Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003329

Physical Location & Seq

Genomic Coordinates Reverse (-)

19846116 .. 19851149

Gene Structure 5034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1113100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGATATTAAGGAAGCAGAGCGTGTAGTTATAGCTAAACCAGTTGCTTCAAGGCCAACTTGTTCCACCTTTAGGTCCTTCACGGAGCTTCTTGCAGGAGCTATTGATGCTTCTCCTTTGAATGTATCTTCTGAAACTGCTGTTCCTGCCATCAGACCAAAGACCGTGAGGTTCAAGCCAATGGTGAATCATACTGTCGCTGGATTGGTTTCTTCCCAGGCACATATCTCTGGTGCAGCACATAGTCATTCATCGGAGCAGGTTTCGAAATCAGATAGCAGATCAACTGTGATATATAAACCATTGGCAAAAGTTGTTTCAAGGGCAACCTTTTCTGTCTTGGCAAATATGGGAAACTTCAATACCAGCCACCAATCTACACAATCATCAGTTAATGTGGGTGTTCTACATCCAAATCAAGATAAATGCTTTAGATCCCAAAGCGCAAATCTGCACCATAATAATCCATCATGTGCCGAGACAAATCAGACAACAGAGCCTCCGAAAATAGCCTCACAAAACATGGAAGAGGATCTGAAACTTATACCATCCACAGCCAATACTGATAGGCCTTCTTATGATGGGTATAATTGGAGAAAATATGGACAGAAGCAAGTCAAAGGAAGTGAGTACCCCCGAAGTTACTATAAGTGCACTCATCCAAATTGTCCTGTGAAAAAGAAGGTTGAACGATCTCTGGATGGCCAGATTGCTGAGATTGTGTACAAGGGGGAACACAACCACTCAAAGCCTCAGCCTCCAAAGCGGAGCTCATCAGGGACGCAAGGGTTAGGCGTACCATCTAATGGAACTGGTCAAGATACCAACAACCGGCTATTGAATAGTCATATTAATGAAAGAAATGAAGGTTCCGAAGGTAGAGTAGAAGATCAAAATGAAGTTGGACTACCTGTGCAGTCTTACCAAACCAAAGCTCCACTACCTTATGATCCCCATGCGACTGGAGGAATAACTGCTGGTGGTGCAACTCCTGATAATTCTTGTGGTCTTAGTGGCGAATGTGAGGAAGCAAGTAAGAGGCTTGAAGCAGAGGATTATGAACCTAGAAGTAAAAGAAGAAAAAGTGAGAATCATTCCCTTGAAGCAGGGATATCAGGGGAAGGTGTCCAAGATCCTCGTGTTGTGGTGCAAAATTCTGTTGAATCTGATATGACGGGTGATGGCTTCCGCTGGAGGAAGTACGGGCAGAAAGTTGTTAAGGGAAATCCATATCCCAGTTATTACAGATGTACCAGTCTCAAATGCAGCGTGCGCAAGCATGTCGAAAGAGTCTCGGATGATCCCAAAGCTTTCATCACCACGTACGAGGGAAAGCACAACCACGACATGCCACTGAGGAACACGAACGCAGGAGCATCTGAGAAGGATACAACAACTAAAGAAAAGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0008544 GO:0009791 GO:0009888 GO:0009889 GO:0009913 GO:0009957 GO:0009987 GO:0010154 GO:0010214 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0030855 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0045165 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0060429 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

471

Amino Acids

51.52

Weight (kDa)

8.93

Isoelectric Point (pI)

47.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	194 - 248	1e-26	WRKY DNA -binding domain
WRKY	PF03106	394 - 449	4.6e-23	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37260 AT2G37260 AT2G37260
fragaria_vesca	FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050
malus_domestica	MD04G1112800.v1.1 MD04G1113100.v1.1 MD12G1128800.v1.1 MD12G1129000.v1.1
prunus_persica	Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1
pyrus_communis	pycom04g10230 pycom12g12530
rosa_chinensis	RchiOBHm_Chr3g0468221
rosa_laevigata	RLG00000024440
rosa_multiflora	Rmu_sc0004524.1_g000004
rosa_roxburghii	Rroxscaffold_6G00412890
rosa_rugosa	Rorug03G0094300 Rorug03G0094400 Rorug03G0094500 Rorug03G0094600 Rorug03G0094600
rosa_samantha	Rh3AG142000 Rh3BG163400 Rh3DG164100
rosa_wichuraiana	Rw3G013440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1274
Acc36I	ACCTGC	1 cut(s)	250
AccB7I	CCANNNNNTGG	1 cut(s)	806
AciI	CCGC	2 cut(s)	766, 1189
AclWI	GGATC	5 cut(s)	429, 540, 944, 1127, 1295
AcoI	YGGCCR	1 cut(s)	703
AcsI	RAATTY	1 cut(s)	1153
AfaI	GTAC	6 cut(s)	632, 725, 798, 1202, 1252, 1325
AfiI	CCNNNNNNNGG	3 cut(s)	72, 806, 831
AjnI	CCWGG	1 cut(s)	216
AluBI	AGCT	6 cut(s)	35, 88, 101, 771, 935, 1310
AluI	AGCT	6 cut(s)	35, 88, 101, 771, 935, 1310
Alw21I	GWGCWC	2 cut(s)	656, 773
Alw26I	GTCTC	3 cut(s)	473, 1262, 1297
Alw44I	GTGCAC	1 cut(s)	652
AlwI	GGATC	5 cut(s)	429, 540, 944, 1127, 1295
AoxI	GGCC	3 cut(s)	53, 569, 703
ApaLI	GTGCAC	1 cut(s)	652
ApeKI	GCWGC	2 cut(s)	236, 1266
ApoI	RAATTY	1 cut(s)	1153
AseI	ATTAAT	1 cut(s)	852
AspLEI	GCGC	2 cut(s)	446, 1275
AspS9I	GGNCC	1 cut(s)	75
AsuHPI	GGTGA	3 cut(s)	196, 1190, 1309
AsuII	TTCGAA	1 cut(s)	266
AvaII	GGWCC	1 cut(s)	75
BaeGI	GKGCMC	1 cut(s)	656
BaeI	ACNNNNGTAYC	2 cut(s)	1234, 1267
BalI	TGGCCA	1 cut(s)	705
BanII	GRGCYC	1 cut(s)	773
BarI	GAAGNNNNNNTAC	2 cut(s)	891, 923
BauI	CACGAG	1 cut(s)	1137
Bbv12I	GWGCWC	2 cut(s)	656, 773
BbvCI	CCTCAGC	1 cut(s)	753
BbvI	GCAGC	2 cut(s)	248, 1278
BccI	CCATC	7 cut(s)	158, 475, 556, 575, 695, 808, 1175
BcgI	CGANNNNNNTGC	2 cut(s)	1264, 1298
BciT130I	CCWGG	1 cut(s)	218
BciVI	GTATCC	1 cut(s)	1381
BcoDI	GTCTC	3 cut(s)	473, 1262, 1297
BfaI	CTAG	1 cut(s)	1065
BfuAI	ACCTGC	1 cut(s)	250
BfuI	GTATCC	1 cut(s)	1381
BisI	GCNGC	2 cut(s)	237, 1267
BlsI	GCNGC	2 cut(s)	238, 1268
Bme1390I	CCNGG	1 cut(s)	218
Bme18I	GGWCC	1 cut(s)	75
BmgT120I	GGNCC	1 cut(s)	75
BmiI	GGNNCC	1 cut(s)	871
BmrFI	CCNGG	1 cut(s)	218
BmrI	ACTGGG	1 cut(s)	1230
BmsI	GCATC	2 cut(s)	97, 1385
BmuI	ACTGGG	1 cut(s)	1230
BpmI	CTGGAG	2 cut(s)	984, 1213
Bpu10I	CCTNAGC	1 cut(s)	753
Bpu14I	TTCGAA	1 cut(s)	266
BsaAI	YACGTR	1 cut(s)	1323
BsaJI	CCNNGG	1 cut(s)	216
BsaXI	ACNNNNNCTCC	4 cut(s)	484, 514, 919, 949
Bsc4I	CCNNNNNNNGG	3 cut(s)	72, 806, 831
Bse118I	RCCGGY	1 cut(s)	831
Bse1I	ACTGG	5 cut(s)	41, 817, 967, 1236, 1254
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	1 cut(s)	216
BseGI	GGATG	5 cut(s)	409, 548, 658, 706, 1303
BseLI	CCNNNNNNNGG	3 cut(s)	72, 806, 831
BseMII	CTCAG	4 cut(s)	705, 767, 1346, 1371
BseNI	ACTGG	5 cut(s)	41, 817, 967, 1236, 1254
BseSI	GKGCMC	1 cut(s)	656
BseXI	GCAGC	2 cut(s)	248, 1278
BsgI	GTGCAG	3 cut(s)	255, 437, 935
BshFI	GGCC	3 cut(s)	55, 571, 705
BsiHKAI	GWGCWC	2 cut(s)	656, 773
BsiSI	CCGG	1 cut(s)	832
BsiWI	CGTACG	1 cut(s)	1323
BslFI	GGGAC	1 cut(s)	793
BslI	CCNNNNNNNGG	3 cut(s)	72, 806, 831
BsmAI	GTCTC	3 cut(s)	473, 1262, 1297
BsmFI	GGGAC	1 cut(s)	793
BsnI	GGCC	3 cut(s)	55, 571, 705
Bsp119I	TTCGAA	1 cut(s)	266
Bsp1286I	GDGCHC	2 cut(s)	656, 773
Bsp1407I	TGTACA	1 cut(s)	723
Bsp143I	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
BspACI	CCGC	2 cut(s)	766, 1189
BspANI	GGCC	3 cut(s)	55, 571, 705
BspCNI	CTCAG	4 cut(s)	706, 766, 1347, 1372
BspLI	GGNNCC	1 cut(s)	871
BspMI	ACCTGC	1 cut(s)	250
BspPI	GGATC	5 cut(s)	429, 540, 944, 1127, 1295
BspT104I	TTCGAA	1 cut(s)	266
BsrFI	RCCGGY	1 cut(s)	831
BsrGI	TGTACA	1 cut(s)	723
BsrI	ACTGG	5 cut(s)	41, 817, 967, 1236, 1254
BssAI	RCCGGY	1 cut(s)	831
BssECI	CCNNGG	1 cut(s)	216
BssMI	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
BssSI	CACGAG	1 cut(s)	1137
Bst2BI	CACGAG	1 cut(s)	1137
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	3 cut(s)	166, 196, 289
Bst6I	CTCTTC	1 cut(s)	522
BstAUI	TGTACA	1 cut(s)	723
BstBAI	YACGTR	1 cut(s)	1323
BstBI	TTCGAA	1 cut(s)	266
BstC8I	GCNNGC	2 cut(s)	1271, 1277
BstDEI	CTNAG	5 cut(s)	714, 753, 1010, 1355, 1380
BstF5I	GGATG	5 cut(s)	409, 548, 658, 706, 1303
BstHHI	GCGC	2 cut(s)	446, 1275
BstKTI	GATC	8 cut(s)	284, 437, 535, 695, 892, 952, 1135, 1303
BstMAI	GTCTC	3 cut(s)	473, 1262, 1297
BstMBI	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
BstMWI	GCNNNNNNNGC	5 cut(s)	107, 146, 763, 983, 1272
BstNI	CCWGG	1 cut(s)	218
BstNSI	RCATGY	2 cut(s)	1283, 1351
BstSCI	CCNGG	1 cut(s)	216
BstSLI	GKGCMC	1 cut(s)	656
BstV1I	GCAGC	2 cut(s)	248, 1278
BstX2I	RGATCY	3 cut(s)	434, 532, 1132
BstXI	CCANNNNNNTGG	1 cut(s)	962
BstYI	RGATCY	3 cut(s)	434, 532, 1132
BsuI	GTATCC	1 cut(s)	1381
BsuRI	GGCC	3 cut(s)	55, 571, 705
BtsCI	GGATG	5 cut(s)	409, 548, 658, 706, 1303
BtsIMutI	CAGTG	1 cut(s)	1352
BveI	ACCTGC	1 cut(s)	250
Cac8I	GCNNGC	2 cut(s)	1271, 1277
CfoI	GCGC	2 cut(s)	446, 1275
Cfr10I	RCCGGY	1 cut(s)	831
Cfr13I	GGNCC	1 cut(s)	75
CseI	GACGC	1 cut(s)	790
Csp6I	GTAC	6 cut(s)	631, 724, 797, 1201, 1251, 1324
CviAII	CATG	6 cut(s)	471, 523, 956, 1280, 1348, 1410
CviQI	GTAC	6 cut(s)	631, 724, 797, 1201, 1251, 1324
DdeI	CTNAG	5 cut(s)	714, 753, 1010, 1355, 1380
DpnI	GATC	8 cut(s)	283, 436, 534, 694, 891, 951, 1134, 1302
DpnII	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
EaeI	YGGCCR	1 cut(s)	703
Eam1104I	CTCTTC	1 cut(s)	522
EarI	CTCTTC	1 cut(s)	522
Ecl136II	GAGCTC	1 cut(s)	771
Eco147I	AGGCCT	1 cut(s)	571
Eco24I	GRGCYC	1 cut(s)	773
Eco32I	GATATC	1 cut(s)	1113
Eco47I	GGWCC	1 cut(s)	75
Eco53kI	GAGCTC	1 cut(s)	771
EcoICRI	GAGCTC	1 cut(s)	771
EcoO109I	RGGNCCY	1 cut(s)	75
EcoRII	CCWGG	1 cut(s)	216
EcoRV	GATATC	1 cut(s)	1113
EcoT38I	GRGCYC	1 cut(s)	773
FaeI	CATG	6 cut(s)	474, 526, 959, 1283, 1351, 1413
FalI	AAGNNNNNCTT	2 cut(s)	43, 75
FaqI	GGGAC	1 cut(s)	793
FatI	CATG	6 cut(s)	470, 522, 955, 1279, 1347, 1409
Fnu4HI	GCNGC	2 cut(s)	237, 1267
FokI	GGATG	5 cut(s)	396, 535, 645, 713, 1310
FriOI	GRGCYC	1 cut(s)	773
Fsp4HI	GCNGC	2 cut(s)	237, 1267
FspBI	CTAG	1 cut(s)	1065
FspI	TGCGCA	1 cut(s)	1274
GlaI	GCGC	2 cut(s)	445, 1274
GluI	GCNGC	2 cut(s)	237, 1267
GsuI	CTGGAG	2 cut(s)	984, 1213
HaeIII	GGCC	3 cut(s)	55, 571, 705
HapII	CCGG	1 cut(s)	832
HgaI	GACGC	1 cut(s)	790
HhaI	GCGC	2 cut(s)	446, 1275
Hin1II	CATG	6 cut(s)	474, 526, 959, 1283, 1351, 1413
Hin6I	GCGC	2 cut(s)	444, 1273
HinP1I	GCGC	2 cut(s)	444, 1273
HindIII	AAGCTT	1 cut(s)	1308
HinfI	GANTC	4 cut(s)	187, 1090, 1163, 1290
HpaII	CCGG	1 cut(s)	832
HphI	GGTGA	3 cut(s)	196, 1190, 1309
Hpy166II	GTNNAC	2 cut(s)	654, 724
Hpy188III	TCNNGA	4 cut(s)	419, 698, 818, 992
Hpy8I	GTNNAC	2 cut(s)	654, 724
HpyAV	CCTTC	7 cut(s)	88, 582, 676, 860, 869, 1115, 1378
HpyCH4III	ACNGT	3 cut(s)	166, 196, 289
HpyCH4IV	ACGT	1 cut(s)	1322
HpyCH4V	TGCA	8 cut(s)	95, 236, 454, 654, 916, 986, 1150, 1266
HpyF10VI	GCNNNNNNNGC	5 cut(s)	107, 146, 763, 983, 1272
HpyF3I	CTNAG	5 cut(s)	714, 753, 1010, 1355, 1380
HpySE526I	ACGT	1 cut(s)	1322
Hsp92II	CATG	6 cut(s)	474, 526, 959, 1283, 1351, 1413
HspAI	GCGC	2 cut(s)	444, 1273
Kzo9I	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
LmnI	GCTCC	6 cut(s)	85, 98, 256, 768, 940, 1373
Lsp1109I	GCAGC	2 cut(s)	248, 1278
LweI	GCATC	2 cut(s)	97, 1385
MaeI	CTAG	1 cut(s)	1065
MaeII	ACGT	1 cut(s)	1322
MaeIII	GTNAC	1 cut(s)	641
MalI	GATC	8 cut(s)	283, 436, 534, 694, 891, 951, 1134, 1302
MboI	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
MboII	GAAGA	5 cut(s)	120, 204, 539, 899, 1089
MflI	RGATCY	3 cut(s)	434, 532, 1132
MhlI	GDGCHC	2 cut(s)	656, 773
MlsI	TGGCCA	1 cut(s)	705
MluCI	AATT	4 cut(s)	589, 664, 997, 1153
MluNI	TGGCCA	1 cut(s)	705
MlyI	GAGTC	1 cut(s)	1299
MmeI	TCCRAC	1 cut(s)	883
Mox20I	TGGCCA	1 cut(s)	705
MscI	TGGCCA	1 cut(s)	705
MseI	TTAA	4 cut(s)	9, 393, 852, 1218
MslI	CAYNNNNRTG	1 cut(s)	852
Msp20I	TGGCCA	1 cut(s)	705
MspA1I	CMGCKG	1 cut(s)	1191
MspI	CCGG	1 cut(s)	832
MspR9I	CCNGG	1 cut(s)	218
MvaI	CCWGG	1 cut(s)	218
MwoI	GCNNNNNNNGC	5 cut(s)	107, 146, 763, 983, 1272
NdeII	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
NlaIII	CATG	6 cut(s)	474, 526, 959, 1283, 1351, 1413
NlaIV	GGNNCC	1 cut(s)	871
NmeAIII	GCCGAG	1 cut(s)	502
NsbI	TGCGCA	1 cut(s)	1274
NspI	RCATGY	2 cut(s)	1283, 1351
NspV	TTCGAA	1 cut(s)	266
PceI	AGGCCT	1 cut(s)	571
PfeI	GAWTC	3 cut(s)	187, 1090, 1163
Pfl23II	CGTACG	1 cut(s)	1323
PflMI	CCANNNNNTGG	1 cut(s)	806
PkrI	GCNGC	2 cut(s)	238, 1268
PleI	GAGTC	1 cut(s)	1298
PpsI	GAGTC	1 cut(s)	1298
Ppu21I	YACGTR	1 cut(s)	1323
PpuMI	RGGWCCY	1 cut(s)	75
PshBI	ATTAAT	1 cut(s)	852
Psp124BI	GAGCTC	1 cut(s)	773
Psp5II	RGGWCCY	1 cut(s)	75
Psp6I	CCWGG	1 cut(s)	216
PspGI	CCWGG	1 cut(s)	216
PspLI	CGTACG	1 cut(s)	1323
PspN4I	GGNNCC	1 cut(s)	871
PspPI	GGNCC	1 cut(s)	75
PspPPI	RGGWCCY	1 cut(s)	75
PsuI	RGATCY	3 cut(s)	434, 532, 1132
RsaI	GTAC	6 cut(s)	632, 725, 798, 1202, 1252, 1325
RsaNI	GTAC	6 cut(s)	631, 724, 797, 1201, 1251, 1324
RseI	CAYNNNNRTG	1 cut(s)	852
SacI	GAGCTC	1 cut(s)	773
SaqAI	TTAA	4 cut(s)	9, 393, 852, 1218
SatI	GCNGC	2 cut(s)	237, 1267
Sau3AI	GATC	8 cut(s)	281, 434, 532, 692, 889, 949, 1132, 1300
Sau96I	GGNCC	1 cut(s)	75
SchI	GAGTC	1 cut(s)	1299
ScrFI	CCNGG	1 cut(s)	218
SduI	GDGCHC	2 cut(s)	656, 773
SfaNI	GCATC	2 cut(s)	97, 1385
SfuI	TTCGAA	1 cut(s)	266
SinI	GGWCC	1 cut(s)	75
SmiMI	CAYNNNNRTG	1 cut(s)	852
Sse9I	AATT	4 cut(s)	589, 664, 997, 1153
SseBI	AGGCCT	1 cut(s)	571
SsiI	CCGC	2 cut(s)	766, 1189
SspMI	CTAG	1 cut(s)	1065
SstI	GAGCTC	1 cut(s)	773
StuI	AGGCCT	1 cut(s)	571
StyD4I	CCNGG	1 cut(s)	216
TaaI	ACNGT	3 cut(s)	166, 196, 289
TaiI	ACGT	1 cut(s)	1325
TaqI	TCGA	2 cut(s)	266, 1284
TasI	AATT	4 cut(s)	589, 664, 997, 1153
TatI	WGTACW	1 cut(s)	723
TfiI	GAWTC	3 cut(s)	187, 1090, 1163
Tru1I	TTAA	4 cut(s)	9, 393, 852, 1218
Tru9I	TTAA	4 cut(s)	9, 393, 852, 1218
TscAI	CASTG	1 cut(s)	1359
TseI	GCWGC	2 cut(s)	236, 1266
TspDTI	ATGAA	6 cut(s)	240, 870, 879, 912, 1074, 1303
TspGWI	ACGGA	1 cut(s)	98
TspRI	CASTG	1 cut(s)	1359
Van91I	CCANNNNNTGG	1 cut(s)	806
VneI	GTGCAC	1 cut(s)	652
VpaK11BI	GGWCC	1 cut(s)	75
VspI	ATTAAT	1 cut(s)	852
XapI	RAATTY	1 cut(s)	1153
XceI	RCATGY	2 cut(s)	1283, 1351
XspI	CTAG	1 cut(s)	1065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.