Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003329

Physical Location & Seq

Genomic Coordinates Forward (+)

20397217 .. 20403016

Gene Structure 5800 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1128800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGATATTAAGGAAGCAGAGCGTGTAGTTATAGCTAAACCAGTTGCTTCAAGGCCTACATGTTCCACCTTTAGGTCCTTCACAGAGCTTCTTGCAGGAGCTATTGATGCCTCTCCCTCTAATGTATCTTCTGAAACTGCTGTTCCTGCCATCAGACCAAAGACCGTGAGGTTCAAGCCATTGGTGAATCATGCTGTCGATGGATTAGTTTCTTCCCAGGTGGATATCTCTGGTGCAGCACATAGACATTCATCGGGGAAGGTTTCGAAACCAGATAGCAGATCAACTGTGGTATATAAACCATTGGCGAAAGTTGTTTCAAGGGCAACCGTTTCTGTCTTGGCAAATATGGGAAACTTCAATACCTGCCACCAATCTACACGATCATCAGTTAATGCGGATGTTCTACGTCCAAATCAAGATAAAAGCTTTAGATCTCAAAGTGTAATTCTGCGCCATAATAATCCATCATGTGCCGAGACAAATCAGACAACAGAGCCTCCGAAAATAGCCTCACAAAACATGGAAGAGGATCCAAAAATTGTACCATCCACAGCCAATACTGATAGGCCTTCTTATGATGGGTATAATTGGAGAAAATATGGACAGAAGCAAGTCAAGGGAAGCGAGTACCCCCGAAGTTACTATAAATGCACGCATCCAAATTGTCCTGTGAAAAAGAAGGTAGAACGATCTCTGGATGGACAGATTGCTGAGATTGTCTACAAGGGTGAACACAACCATTCGAAGCCTCAACCTCCAAAGCGTGGCTCATCAGGAACGCAAGGGTTAGGTGTAGCCTCTGATGGAACTGGTCAAGATACCAACAACAGGTTATGTAATAGTCATCATAATGAAAGAAATGATGGTTCGGAAGTTAGATTAGAAGATCAGAATGAAGTTGGACTGCCGGTGCATTTTTACCAAAGCAAAGCTCTACTACCTTATGATCCCCTAGCAAGTGGAGGAATAAATGGTGGTGGTGCAACTCCTGATAATTCTTGTGGTCTTAGTGGAGAATGCGAGGAAGGAAGCAAGGGGCTTGAAGCAGAGGATTGTGAGCCTAGAAGTAAAAGAAGAAAAAGTGAGAATCAATCCAATGAAGCAGGGATATCAGGGGAAGGTGTCCAAGATCCCCGTGTTGTGGTGCAAAGTTCAGTTGAATCTGATATGACGGGTGATGGCTTCCGCTGGAGGAAGTACGGGCAGAAGGTTGTTAAGGGGAATCCATATCCCAGAAGTTACTACAGATGTACCAGTCTCAAATGCAATGTGCGTAAGCATGTCGAAAGAGTCTCGGATGATCCCAAAGCTTTCATCACAACGTACGAGGGAAAGCACAACCACGACATGCCACTGAGGAACGCGAACACAGGAGCATCCGAAAAGGATACAATCAAAGAAAAGCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0008544 GO:0009791 GO:0009888 GO:0009889 GO:0009913 GO:0009957 GO:0009987 GO:0010154 GO:0010214 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0030855 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0045165 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0060429 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

471

Amino Acids

51.45

Weight (kDa)

9.0

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	194 - 248	1e-26	WRKY DNA -binding domain
WRKY	PF03106	394 - 450	1.9e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37260 AT2G37260 AT2G37260
fragaria_vesca	FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050
malus_domestica	MD04G1112800.v1.1 MD04G1113100.v1.1 MD12G1128800.v1.1 MD12G1129000.v1.1
prunus_persica	Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1
pyrus_communis	pycom04g10230 pycom12g12530
rosa_chinensis	RchiOBHm_Chr3g0468221
rosa_laevigata	RLG00000024440
rosa_multiflora	Rmu_sc0004524.1_g000004
rosa_roxburghii	Rroxscaffold_6G00412890
rosa_rugosa	Rorug03G0094300 Rorug03G0094400 Rorug03G0094500 Rorug03G0094600 Rorug03G0094600
rosa_samantha	Rh3AG142000 Rh3BG163400 Rh3DG164100
rosa_wichuraiana	Rw3G013440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	374
AccB7I	CCANNNNNTGG	1 cut(s)	767
AccI	GTMKAC	1 cut(s)	723
AccII	CGCG	1 cut(s)	1367
AciI	CCGC	2 cut(s)	398, 1189
AclWI	GGATC	5 cut(s)	527, 540, 944, 1127, 1298
AfaI	GTAC	5 cut(s)	546, 632, 1202, 1255, 1328
AfiI	CCNNNNNNNGG	4 cut(s)	72, 767, 831, 1144
AflIII	ACRYGT	1 cut(s)	59
AgsI	TTSAA	6 cut(s)	51, 175, 321, 361, 1046, 1163
AjnI	CCWGG	1 cut(s)	216
AluBI	AGCT	6 cut(s)	35, 88, 101, 429, 935, 1313
AluI	AGCT	6 cut(s)	35, 88, 101, 429, 935, 1313
Alw26I	GTCTC	3 cut(s)	473, 1265, 1300
AlwI	GGATC	5 cut(s)	527, 540, 944, 1127, 1298
AoxI	GGCC	2 cut(s)	53, 569
ApeKI	GCWGC	1 cut(s)	236
AspLEI	GCGC	1 cut(s)	456
AspS9I	GGNCC	1 cut(s)	75
AsuHPI	GGTGA	3 cut(s)	196, 743, 1190
AsuII	TTCGAA	2 cut(s)	266, 746
AvaII	GGWCC	1 cut(s)	75
BaeI	ACNNNNGTAYC	2 cut(s)	1237, 1270
BamHI	GGATCC	1 cut(s)	532
BbvI	GCAGC	1 cut(s)	248
BccI	CCATC	9 cut(s)	158, 194, 475, 556, 575, 695, 800, 860, 1175
BciT130I	CCWGG	1 cut(s)	218
BciVI	GTATCC	1 cut(s)	1384
BcoDI	GTCTC	3 cut(s)	473, 1265, 1300
BfaI	CTAG	2 cut(s)	956, 1065
BfmI	CTRYAG	1 cut(s)	1246
BfuAI	ACCTGC	1 cut(s)	374
BfuI	GTATCC	1 cut(s)	1384
BglII	AGATCT	1 cut(s)	434
BisI	GCNGC	1 cut(s)	237
BlsI	GCNGC	1 cut(s)	238
Bme1390I	CCNGG	1 cut(s)	218
Bme18I	GGWCC	1 cut(s)	75
BmgT120I	GGNCC	1 cut(s)	75
BmiI	GGNNCC	1 cut(s)	534
BmrFI	CCNGG	1 cut(s)	218
BmsI	GCATC	3 cut(s)	97, 667, 1388
BpmI	CTGGAG	1 cut(s)	1213
Bpu14I	TTCGAA	2 cut(s)	266, 746
BsaJI	CCNNGG	1 cut(s)	216
BsaXI	ACNNNNNCTCC	2 cut(s)	484, 514
Bsc4I	CCNNNNNNNGG	4 cut(s)	72, 767, 831, 1144
Bse118I	RCCGGY	1 cut(s)	910
Bse1I	ACTGG	3 cut(s)	41, 817, 1257
Bse3DI	GCAATG	1 cut(s)	1276
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	1 cut(s)	216
BseGI	GGATG	6 cut(s)	406, 548, 658, 706, 1306, 1379
BseLI	CCNNNNNNNGG	4 cut(s)	72, 767, 831, 1144
BseMI	GCAATG	1 cut(s)	1276
BseMII	CTCAG	2 cut(s)	705, 1349
BseNI	ACTGG	3 cut(s)	41, 817, 1257
BseXI	GCAGC	1 cut(s)	248
BsgI	GTGCAG	1 cut(s)	255
Bsh1236I	CGCG	1 cut(s)	1367
BshFI	GGCC	2 cut(s)	55, 571
BsiSI	CCGG	1 cut(s)	911
BsiWI	CGTACG	1 cut(s)	1326
BslI	CCNNNNNNNGG	4 cut(s)	72, 767, 831, 1144
BsmAI	GTCTC	3 cut(s)	473, 1265, 1300
BsmI	GAATGC	1 cut(s)	1025
BsnI	GGCC	2 cut(s)	55, 571
Bsp119I	TTCGAA	2 cut(s)	266, 746
Bsp143I	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
BspACI	CCGC	2 cut(s)	398, 1189
BspANI	GGCC	2 cut(s)	55, 571
BspCNI	CTCAG	2 cut(s)	706, 1350
BspFNI	CGCG	1 cut(s)	1367
BspLI	GGNNCC	1 cut(s)	534
BspMI	ACCTGC	1 cut(s)	374
BspPI	GGATC	5 cut(s)	527, 540, 944, 1127, 1298
BspT104I	TTCGAA	2 cut(s)	266, 746
BsrDI	GCAATG	1 cut(s)	1276
BsrFI	RCCGGY	1 cut(s)	910
BsrI	ACTGG	3 cut(s)	41, 817, 1257
BssAI	RCCGGY	1 cut(s)	910
BssECI	CCNNGG	1 cut(s)	216
BssMI	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	3 cut(s)	166, 289, 331
Bst6I	CTCTTC	1 cut(s)	522
BstBI	TTCGAA	2 cut(s)	266, 746
BstC8I	GCNNGC	1 cut(s)	656
BstDEI	CTNAG	3 cut(s)	714, 1010, 1358
BstF5I	GGATG	6 cut(s)	406, 548, 658, 706, 1306, 1379
BstFNI	CGCG	1 cut(s)	1367
BstHHI	GCGC	1 cut(s)	456
BstKTI	GATC	9 cut(s)	284, 386, 437, 535, 695, 892, 952, 1135, 1306
BstMAI	GTCTC	3 cut(s)	473, 1265, 1300
BstMBI	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
BstMWI	GCNNNNNNNGC	2 cut(s)	107, 146
BstNI	CCWGG	1 cut(s)	218
BstNSI	RCATGY	3 cut(s)	63, 1286, 1354
BstSCI	CCNGG	1 cut(s)	216
BstSFI	CTRYAG	1 cut(s)	1246
BstUI	CGCG	1 cut(s)	1367
BstV1I	GCAGC	1 cut(s)	248
BstX2I	RGATCY	3 cut(s)	434, 532, 1132
BstYI	RGATCY	3 cut(s)	434, 532, 1132
BsuI	GTATCC	1 cut(s)	1384
BsuRI	GGCC	2 cut(s)	55, 571
BtsCI	GGATG	6 cut(s)	406, 548, 658, 706, 1306, 1379
BtsIMutI	CAGTG	1 cut(s)	1355
BveI	ACCTGC	1 cut(s)	374
Cac8I	GCNNGC	1 cut(s)	656
CfoI	GCGC	1 cut(s)	456
Cfr10I	RCCGGY	1 cut(s)	910
Cfr13I	GGNCC	1 cut(s)	75
Csp6I	GTAC	5 cut(s)	545, 631, 1201, 1254, 1327
CviAII	CATG	6 cut(s)	60, 191, 471, 523, 1283, 1351
CviQI	GTAC	5 cut(s)	545, 631, 1201, 1254, 1327
DdeI	CTNAG	3 cut(s)	714, 1010, 1358
DpnI	GATC	9 cut(s)	283, 385, 436, 534, 694, 891, 951, 1134, 1305
DpnII	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
Eam1104I	CTCTTC	1 cut(s)	522
EarI	CTCTTC	1 cut(s)	522
Eco147I	AGGCCT	2 cut(s)	55, 571
Eco32I	GATATC	2 cut(s)	226, 1113
Eco47I	GGWCC	1 cut(s)	75
EcoO109I	RGGNCCY	1 cut(s)	75
EcoRII	CCWGG	1 cut(s)	216
EcoRV	GATATC	2 cut(s)	226, 1113
FaeI	CATG	6 cut(s)	63, 194, 474, 526, 1286, 1354
FatI	CATG	6 cut(s)	59, 190, 470, 522, 1282, 1350
FblI	GTMKAC	1 cut(s)	723
Fnu4HI	GCNGC	1 cut(s)	237
FokI	GGATG	6 cut(s)	413, 535, 645, 713, 1313, 1366
Fsp4HI	GCNGC	1 cut(s)	237
FspBI	CTAG	2 cut(s)	956, 1065
GlaI	GCGC	1 cut(s)	455
GluI	GCNGC	1 cut(s)	237
GsuI	CTGGAG	1 cut(s)	1213
HaeIII	GGCC	2 cut(s)	55, 571
HapII	CCGG	1 cut(s)	911
HhaI	GCGC	1 cut(s)	456
Hin1II	CATG	6 cut(s)	63, 194, 474, 526, 1286, 1354
Hin6I	GCGC	1 cut(s)	454
HinP1I	GCGC	1 cut(s)	454
HindIII	AAGCTT	2 cut(s)	427, 1311
HinfI	GANTC	5 cut(s)	187, 1090, 1163, 1225, 1293
HpaII	CCGG	1 cut(s)	911
HphI	GGTGA	3 cut(s)	196, 743, 1190
Hpy166II	GTNNAC	2 cut(s)	724, 734
Hpy188III	TCNNGA	5 cut(s)	419, 698, 777, 818, 992
Hpy8I	GTNNAC	2 cut(s)	724, 734
HpyAV	CCTTC	7 cut(s)	88, 253, 582, 676, 1022, 1115, 1204
HpyCH4III	ACNGT	3 cut(s)	166, 289, 331
HpyCH4IV	ACGT	2 cut(s)	409, 1325
HpyCH4V	TGCA	7 cut(s)	95, 236, 654, 916, 986, 1150, 1269
HpyF10VI	GCNNNNNNNGC	2 cut(s)	107, 146
HpyF3I	CTNAG	3 cut(s)	714, 1010, 1358
HpySE526I	ACGT	2 cut(s)	409, 1325
Hsp92II	CATG	6 cut(s)	63, 194, 474, 526, 1286, 1354
HspAI	GCGC	1 cut(s)	454
Kzo9I	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
LmnI	GCTCC	2 cut(s)	98, 1376
Lsp1109I	GCAGC	1 cut(s)	248
LweI	GCATC	3 cut(s)	97, 667, 1388
MaeI	CTAG	2 cut(s)	956, 1065
MaeII	ACGT	2 cut(s)	409, 1325
MaeIII	GTNAC	2 cut(s)	641, 1241
MalI	GATC	9 cut(s)	283, 385, 436, 534, 694, 891, 951, 1134, 1305
MboI	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
MboII	GAAGA	5 cut(s)	120, 204, 539, 899, 1089
MflI	RGATCY	3 cut(s)	434, 532, 1132
MluCI	AATT	5 cut(s)	447, 540, 589, 664, 997
MlyI	GAGTC	1 cut(s)	1302
MmeI	TCCRAC	1 cut(s)	883
MseI	TTAA	3 cut(s)	9, 393, 1218
MslI	CAYNNNNRTG	1 cut(s)	852
MspA1I	CMGCKG	1 cut(s)	1191
MspI	CCGG	1 cut(s)	911
MspR9I	CCNGG	1 cut(s)	218
Mva1269I	GAATGC	1 cut(s)	1025
MvaI	CCWGG	1 cut(s)	218
MvnI	CGCG	1 cut(s)	1367
MwoI	GCNNNNNNNGC	2 cut(s)	107, 146
NdeII	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
NlaIII	CATG	6 cut(s)	63, 194, 474, 526, 1286, 1354
NlaIV	GGNNCC	1 cut(s)	534
NmeAIII	GCCGAG	1 cut(s)	502
NspI	RCATGY	3 cut(s)	63, 1286, 1354
NspV	TTCGAA	2 cut(s)	266, 746
PceI	AGGCCT	2 cut(s)	55, 571
PciI	ACATGT	1 cut(s)	59
PctI	GAATGC	1 cut(s)	1025
PfeI	GAWTC	4 cut(s)	187, 1090, 1163, 1225
Pfl23II	CGTACG	1 cut(s)	1326
PflMI	CCANNNNNTGG	1 cut(s)	767
PkrI	GCNGC	1 cut(s)	238
PleI	GAGTC	1 cut(s)	1301
PpsI	GAGTC	1 cut(s)	1301
PpuMI	RGGWCCY	1 cut(s)	75
PscI	ACATGT	1 cut(s)	59
Psp5II	RGGWCCY	1 cut(s)	75
Psp6I	CCWGG	1 cut(s)	216
PspGI	CCWGG	1 cut(s)	216
PspLI	CGTACG	1 cut(s)	1326
PspN4I	GGNNCC	1 cut(s)	534
PspPI	GGNCC	1 cut(s)	75
PspPPI	RGGWCCY	1 cut(s)	75
PsuI	RGATCY	3 cut(s)	434, 532, 1132
RsaI	GTAC	5 cut(s)	546, 632, 1202, 1255, 1328
RsaNI	GTAC	5 cut(s)	545, 631, 1201, 1254, 1327
RseI	CAYNNNNRTG	1 cut(s)	852
SaqAI	TTAA	3 cut(s)	9, 393, 1218
SatI	GCNGC	1 cut(s)	237
Sau3AI	GATC	9 cut(s)	281, 383, 434, 532, 692, 889, 949, 1132, 1303
Sau96I	GGNCC	1 cut(s)	75
SchI	GAGTC	1 cut(s)	1302
ScrFI	CCNGG	1 cut(s)	218
SfaNI	GCATC	3 cut(s)	97, 667, 1388
SfcI	CTRYAG	1 cut(s)	1246
SfuI	TTCGAA	2 cut(s)	266, 746
SinI	GGWCC	1 cut(s)	75
SmiMI	CAYNNNNRTG	1 cut(s)	852
Sse9I	AATT	5 cut(s)	447, 540, 589, 664, 997
SseBI	AGGCCT	2 cut(s)	55, 571
SsiI	CCGC	2 cut(s)	398, 1189
SspMI	CTAG	2 cut(s)	956, 1065
StuI	AGGCCT	2 cut(s)	55, 571
StyD4I	CCNGG	1 cut(s)	216
TaaI	ACNGT	3 cut(s)	166, 289, 331
TaiI	ACGT	2 cut(s)	412, 1328
TaqI	TCGA	4 cut(s)	198, 266, 746, 1287
TasI	AATT	5 cut(s)	447, 540, 589, 664, 997
TfiI	GAWTC	4 cut(s)	187, 1090, 1163, 1225
Tru1I	TTAA	3 cut(s)	9, 393, 1218
Tru9I	TTAA	3 cut(s)	9, 393, 1218
TscAI	CASTG	1 cut(s)	1362
TseI	GCWGC	1 cut(s)	236
TspDTI	ATGAA	5 cut(s)	240, 870, 912, 1116, 1306
TspRI	CASTG	1 cut(s)	1362
Van91I	CCANNNNNTGG	1 cut(s)	767
VpaK11BI	GGWCC	1 cut(s)	75
XceI	RCATGY	3 cut(s)	63, 1286, 1354
XmiI	GTMKAC	1 cut(s)	723
XspI	CTAG	2 cut(s)	956, 1065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.