Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003329

Physical Location & Seq

Genomic Coordinates Forward (+)

20418703 .. 20419358

Gene Structure 656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 243 bp

ATGACGGGTGATGGCTTCCGCTGGAGGAAGTACGGGCAGAAGGTTGTTAAGGGGAATCCATATCCCAGAAGTTACTACAGATGTACCAGTCTCAAATGCAATGTGCGTAAGCATGTCGAAAGAGTATCGGATGATCCCAAAGCTTTCATCACAACGTACGAGGGAAAGCACAACCACGACATACCACTGAGGAACGCACACACAGGAGCATCCGATAAGGATACAACTAAACAAAAGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001708 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0008544 GO:0009791 GO:0009888 GO:0009889 GO:0009913 GO:0009957 GO:0009987 GO:0010154 GO:0010214 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0030855 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0045165 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0060429 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

81

Amino Acids

9.28

Weight (kDa)

9.85

Isoelectric Point (pI)

18.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	4 - 60	4.9e-28	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003329)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37260 AT2G37260 AT2G37260
fragaria_vesca	FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050 FvH4_6g15050
malus_domestica	MD04G1112800.v1.1 MD04G1113100.v1.1 MD12G1128800.v1.1 MD12G1129000.v1.1
prunus_persica	Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1 Prupe.6G244300_v2.0.a1
pyrus_communis	pycom04g10230 pycom12g12530
rosa_chinensis	RchiOBHm_Chr3g0468221
rosa_laevigata	RLG00000024440
rosa_multiflora	Rmu_sc0004524.1_g000004
rosa_roxburghii	Rroxscaffold_6G00412890
rosa_rugosa	Rorug03G0094300 Rorug03G0094400 Rorug03G0094500 Rorug03G0094600 Rorug03G0094600
rosa_samantha	Rh3AG142000 Rh3BG163400 Rh3DG164100
rosa_wichuraiana	Rw3G013440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	19
AclWI	GGATC	1 cut(s)	128
AfaI	GTAC	3 cut(s)	32, 85, 158
AluBI	AGCT	1 cut(s)	143
AluI	AGCT	1 cut(s)	143
Alw26I	GTCTC	1 cut(s)	95
AlwI	GGATC	1 cut(s)	128
AsuHPI	GGTGA	1 cut(s)	20
BaeI	ACNNNNGTAYC	2 cut(s)	67, 100
BccI	CCATC	1 cut(s)	5
BciVI	GTATCC	1 cut(s)	214
BcoDI	GTCTC	1 cut(s)	95
BfmI	CTRYAG	1 cut(s)	76
BfuI	GTATCC	1 cut(s)	214
BmsI	GCATC	1 cut(s)	218
BpmI	CTGGAG	1 cut(s)	43
Bse1I	ACTGG	1 cut(s)	87
Bse3DI	GCAATG	1 cut(s)	106
BseGI	GGATG	2 cut(s)	136, 209
BseMI	GCAATG	1 cut(s)	106
BseMII	CTCAG	1 cut(s)	179
BseNI	ACTGG	1 cut(s)	87
BsiWI	CGTACG	1 cut(s)	156
BsmAI	GTCTC	1 cut(s)	95
Bsp143I	GATC	1 cut(s)	133
BspACI	CCGC	1 cut(s)	19
BspCNI	CTCAG	1 cut(s)	180
BspPI	GGATC	1 cut(s)	128
BsrDI	GCAATG	1 cut(s)	106
BsrI	ACTGG	1 cut(s)	87
BssMI	GATC	1 cut(s)	133
BstDEI	CTNAG	1 cut(s)	188
BstF5I	GGATG	2 cut(s)	136, 209
BstKTI	GATC	1 cut(s)	136
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	1 cut(s)	133
BstNSI	RCATGY	1 cut(s)	116
BstSFI	CTRYAG	1 cut(s)	76
BsuI	GTATCC	1 cut(s)	214
BtsCI	GGATG	2 cut(s)	136, 209
BtsIMutI	CAGTG	1 cut(s)	185
Csp6I	GTAC	3 cut(s)	31, 84, 157
CviAII	CATG	2 cut(s)	113, 240
CviJI	RGCY	3 cut(s)	15, 143, 238
CviKI_1	RGCY	3 cut(s)	15, 143, 238
CviQI	GTAC	3 cut(s)	31, 84, 157
DdeI	CTNAG	1 cut(s)	188
DpnI	GATC	1 cut(s)	135
DpnII	GATC	1 cut(s)	133
FaeI	CATG	2 cut(s)	116, 243
FaiI	YATR	4 cut(s)	61, 114, 182, 241
FatI	CATG	2 cut(s)	112, 239
FokI	GGATG	2 cut(s)	143, 196
GsuI	CTGGAG	1 cut(s)	43
Hin1II	CATG	2 cut(s)	116, 243
HindIII	AAGCTT	1 cut(s)	141
HinfI	GANTC	1 cut(s)	55
HphI	GGTGA	1 cut(s)	20
Hpy188I	TCNGA	2 cut(s)	130, 214
HpyAV	CCTTC	1 cut(s)	34
HpyCH4IV	ACGT	1 cut(s)	155
HpyCH4V	TGCA	1 cut(s)	99
HpyF3I	CTNAG	1 cut(s)	188
HpySE526I	ACGT	1 cut(s)	155
Hsp92II	CATG	2 cut(s)	116, 243
Kzo9I	GATC	1 cut(s)	133
LmnI	GCTCC	1 cut(s)	206
LpnPI	CCDG	4 cut(s)	7, 79, 100, 189
LweI	GCATC	1 cut(s)	218
MaeII	ACGT	1 cut(s)	155
MaeIII	GTNAC	1 cut(s)	71
MalI	GATC	1 cut(s)	135
MboI	GATC	1 cut(s)	133
MnlI	CCTC	3 cut(s)	18, 154, 183
MseI	TTAA	1 cut(s)	48
MspA1I	CMGCKG	1 cut(s)	21
NdeII	GATC	1 cut(s)	133
NlaIII	CATG	2 cut(s)	116, 243
NspI	RCATGY	1 cut(s)	116
PfeI	GAWTC	1 cut(s)	55
Pfl23II	CGTACG	1 cut(s)	156
PspLI	CGTACG	1 cut(s)	156
RsaI	GTAC	3 cut(s)	32, 85, 158
RsaNI	GTAC	3 cut(s)	31, 84, 157
SaqAI	TTAA	1 cut(s)	48
Sau3AI	GATC	1 cut(s)	133
SetI	ASST	3 cut(s)	45, 145, 158
SfaNI	GCATC	1 cut(s)	218
SfcI	CTRYAG	1 cut(s)	76
SgeI	CNNG	9 cut(s)	18, 34, 46, 78, 99, 125, 172, 188, 216
SsiI	CCGC	1 cut(s)	19
TaiI	ACGT	1 cut(s)	158
TaqI	TCGA	1 cut(s)	117
TfiI	GAWTC	1 cut(s)	55
Tru1I	TTAA	1 cut(s)	48
Tru9I	TTAA	1 cut(s)	48
TscAI	CASTG	1 cut(s)	192
TspDTI	ATGAA	1 cut(s)	136
TspRI	CASTG	1 cut(s)	192
XceI	RCATGY	1 cut(s)	116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.