Rorug03G0094400
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003329
Physical Location & Seq
Reverse (-)
7384151 .. 7384594
444 bp
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UTR
Exon/CDS
Intron
Rorug03G0094400.1

Sequence Viewer

Length: 444 bp
ATGGTGATTCAAGAGAGTCTGCGTCTTTATCCACCAGCAACAATAACAGCCAAGCATGCTTTAGCAGACATAAAACTAGGGGAGCTTGATGTGCCAGGAGGCACCAACATATGGATCTTCGCACACAGATTGCACCGCGACCCTGAAAATTGGGGGCCAGATGCTAATGAGTTTAAGCCAGAGAGATTTGCAAATGGGGTTTCCAAATCGTGCAAGTATACCCAAGCATATGTGCCATTTGGTTTCGGGGCTCGAATTTGCATAGGCCAGAACTTTGCTATGAAGCAAATGAAGATAATACTGTCCCTTATCCTTTCCAAGTTCTCATTTTCTATTTCTCCGAATTATCACCATTCTCCTGTCTCTCCAATGTTACTATTGCCTGGACATGGAATTAGGCTCCTTGTTAGTCCTGTGCAAGCAAAAGCCAATTCGCTGGTTTAA

Protein Analysis

147

Amino Acids

16.3

Weight (kDa)

9.55

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 130 5.3e-37 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 111
AccI GTMKAC 1 cut(s) 218
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 1 cut(s) 255
AfiI CCNNNNNNNGG 2 cut(s) 111, 389
AgsI TTSAA 1 cut(s) 11
AjnI CCWGG 2 cut(s) 94, 382
AluBI AGCT 1 cut(s) 85
AluI AGCT 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 122
AoxI GGCC 2 cut(s) 155, 265
ApoI RAATTY 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 16, 341
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 253
BciT130I CCWGG 2 cut(s) 96, 384
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 96, 384
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 3 cut(s) 103, 156, 401
BmrFI CCNGG 2 cut(s) 96, 384
BmsI GCATC 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 389
BseBI CCWGG 2 cut(s) 96, 384
BseLI CCNNNNNNNGG 2 cut(s) 111, 389
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 2 cut(s) 157, 267
BshNI GGYRCC 1 cut(s) 101
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 2 cut(s) 111, 389
BsmAI GTCTC 1 cut(s) 367
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 2 cut(s) 157, 267
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 1 cut(s) 136
BspANI GGCC 2 cut(s) 157, 267
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 103, 156, 401
BspPI GGATC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 101
BssMI GATC 1 cut(s) 114
BssNAI GTATAC 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 96, 384
Bst4CI ACNGT 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 57, 420
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 56, 91
BstNI CCWGG 2 cut(s) 96, 384
BstNSI RCATGY 1 cut(s) 59
BstSCI CCNGG 2 cut(s) 94, 382
BstUI CGCG 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 1 cut(s) 114
BstZ17I GTATAC 1 cut(s) 219
BsuRI GGCC 2 cut(s) 157, 267
Cac8I GCNNGC 2 cut(s) 57, 420
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 1 cut(s) 11
CviAII CATG 2 cut(s) 56, 389
CviJI RGCY 8 cut(s) 50, 85, 157, 178, 251, 267, 400, 428
CviKI_1 RGCY 8 cut(s) 50, 85, 157, 178, 251, 267, 400, 428
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 94, 382
EcoT38I GRGCYC 1 cut(s) 253
FaeI CATG 2 cut(s) 59, 392
FaqI GGGAC 1 cut(s) 289
FatI CATG 2 cut(s) 55, 388
FauNDI CATATG 2 cut(s) 110, 229
FblI GTMKAC 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 253
FspBI CTAG 1 cut(s) 77
HaeIII GGCC 2 cut(s) 157, 267
HgaI GACGC 1 cut(s) 11
Hin1II CATG 2 cut(s) 59, 392
HinfI GANTC 2 cut(s) 7, 16
HphI GGTGA 2 cut(s) 16, 341
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 1 cut(s) 11
Hpy8I GTNNAC 1 cut(s) 219
HpyCH4III ACNGT 1 cut(s) 303
HpyCH4V TGCA 5 cut(s) 133, 191, 213, 261, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 91
Hsp92II CATG 2 cut(s) 59, 392
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 82, 405
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 1 cut(s) 372
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 2 cut(s) 109, 304
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 5 cut(s) 148, 255, 343, 393, 430
MlyI GAGTC 1 cut(s) 25
MnlI CCTC 1 cut(s) 92
MseI TTAA 2 cut(s) 174, 442
MspR9I CCNGG 2 cut(s) 96, 384
MvaI CCWGG 2 cut(s) 96, 384
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 56, 91
NdeI CATATG 2 cut(s) 110, 229
NdeII GATC 1 cut(s) 114
NlaIII CATG 2 cut(s) 59, 392
NlaIV GGNNCC 3 cut(s) 103, 156, 401
NspI RCATGY 1 cut(s) 59
PaeI GCATGC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 111
PleI GAGTC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 94, 382
PspGI CCWGG 2 cut(s) 94, 382
PspN4I GGNNCC 3 cut(s) 103, 156, 401
PspPI GGNCC 1 cut(s) 155
PsuI RGATCY 1 cut(s) 114
SaqAI TTAA 2 cut(s) 174, 442
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 96, 384
SduI GDGCHC 1 cut(s) 253
SetI ASST 1 cut(s) 87
SfaNI GCATC 1 cut(s) 151
SphI GCATGC 1 cut(s) 59
Sse9I AATT 5 cut(s) 148, 255, 343, 393, 430
SsiI CCGC 1 cut(s) 136
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 94, 382
TaaI ACNGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 253
TasI AATT 5 cut(s) 148, 255, 343, 393, 430
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 174, 442
Tru9I TTAA 2 cut(s) 174, 442
TspDTI ATGAA 2 cut(s) 296, 305
Van91I CCANNNNNTGG 1 cut(s) 111
XapI RAATTY 1 cut(s) 255
XceI RCATGY 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 218
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.