MD04G1133300.v1.1

Belongs to the cyclin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010288
Physical Location & Seq
Reverse (-)
22000002 .. 22001837
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1133300.v1.1.491

Sequence Viewer

Length: 648 bp
ATGATTTGCATAGAAAGTTTGCTTTGCGATGAGGTATGCCCTTCAAGCCCTGCAGCGACTACTGAAACTATCCCTGGCAATAACTTTGGTTCAAAAAGTTACTGTAGATCAATATTAATGTATACAACTAAGGAAGAGTATGAGCAGGCTCTGACCATATGCATGGAGAAGGAGAAGAGCTATATGCCTGAACCTAATTACCTCGATAACCTGTGTTCGAATAATTTGGTCATTGCTAGGTTAAAAAGCACTCAATGGTTCATCAAGTCTTCAAGTCGGTTGAACCTCTCCCTTGGAACTGTTTTTTATGCTGCAAATTACCTTGATCGGTTCATATCCACGAACCATTGCAATGGATGGAAATTTGGATGGTGGAATTGGTGTCTATTGCCTGCTTATCTATTGCCTCCAAGTTCAGTGATACCTACAATCCTTCCATGCTTGAAATTCAGCACAATCCAACGGGTGGAAATGATGGTGCTAAATGCATTAGGATGGCGCCTTGCTTCTACAACGGCTTACTCTTACCTAGAACTGTTTTCATGGTTTCTGGACTCATTGAAGCCCCAACTTCATGAAGAGATCATTGCCCTACTCAACAATTTGCTTCTTCGAGCTATTTCGGGGCGAGTTGGTGAAGTGCCATAA

Protein Analysis

216

Amino Acids

24.49

Weight (kDa)

6.14

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 53 - 117 2.1e-06 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 498
AccB7I CCANNNNNTGG 1 cut(s) 466
AccI GTMKAC 1 cut(s) 122
AcsI RAATTY 2 cut(s) 362, 446
AcyI GRCGYC 1 cut(s) 499
AfiI CCNNNNNNNGG 1 cut(s) 466
AgsI TTSAA 6 cut(s) 45, 93, 273, 283, 445, 562
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 2 cut(s) 180, 617
AluI AGCT 2 cut(s) 180, 617
AlwNI CAGNNNCTG 1 cut(s) 151
ApeKI GCWGC 2 cut(s) 53, 311
ApoI RAATTY 2 cut(s) 362, 446
AseI ATTAAT 1 cut(s) 116
AspLEI GCGC 1 cut(s) 501
AsuHPI GGTGA 1 cut(s) 647
AsuII TTCGAA 1 cut(s) 218
BanI GGYRCC 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 65, 298
BccI CCATC 4 cut(s) 351, 363, 469, 489
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 1 cut(s) 75
BfaI CTAG 2 cut(s) 237, 530
BfmI CTRYAG 2 cut(s) 51, 103
BfoI RGCGCY 1 cut(s) 502
BisI GCNGC 2 cut(s) 54, 312
BlsI GCNGC 2 cut(s) 55, 313
Bme1390I CCNGG 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 500
BmrFI CCNGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 73, 292
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse3DI GCAATG 4 cut(s) 231, 346, 358, 585
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 73, 292
BseGI GGATG 3 cut(s) 362, 374, 500
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMI GCAATG 4 cut(s) 231, 346, 358, 585
BseXI GCAGC 2 cut(s) 65, 298
BshNI GGYRCC 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 218
Bsp143I GATC 3 cut(s) 107, 325, 582
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 500
BspMAI CTGCAG 1 cut(s) 55
BspQI GCTCTTC 1 cut(s) 170
BspT104I TTCGAA 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 498
BsrDI GCAATG 4 cut(s) 231, 346, 358, 585
BssECI CCNNGG 2 cut(s) 73, 292
BssMI GATC 3 cut(s) 107, 325, 582
BssNAI GTATAC 1 cut(s) 123
BssNI GRCGYC 1 cut(s) 499
BssT1I CCWWGG 1 cut(s) 292
Bst1107I GTATAC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 3 cut(s) 104, 301, 537
Bst6I CTCTTC 3 cut(s) 129, 170, 573
BstACI GRCGYC 1 cut(s) 499
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 147, 393
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 3 cut(s) 362, 374, 500
BstH2I RGCGCY 1 cut(s) 502
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 3 cut(s) 110, 328, 585
BstMBI GATC 3 cut(s) 107, 325, 582
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 2 cut(s) 51, 103
BstV1I GCAGC 2 cut(s) 65, 298
BstV2I GAAGAC 1 cut(s) 261
BstXI CCANNNNNNTGG 2 cut(s) 163, 353
BstZ17I GTATAC 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 3 cut(s) 362, 374, 500
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 2 cut(s) 147, 393
CaiI CAGNNNCTG 1 cut(s) 151
CciI TCATGA 1 cut(s) 574
CfoI GCGC 1 cut(s) 501
CviAII CATG 4 cut(s) 163, 438, 543, 575
CviJI RGCY 6 cut(s) 48, 149, 180, 518, 565, 617
CviKI_1 RGCY 6 cut(s) 48, 149, 180, 518, 565, 617
DdeI CTNAG 1 cut(s) 129
DinI GGCGCC 1 cut(s) 500
DpnI GATC 3 cut(s) 109, 327, 584
DpnII GATC 3 cut(s) 107, 325, 582
Eam1104I CTCTTC 3 cut(s) 129, 170, 573
EarI CTCTTC 3 cut(s) 129, 170, 573
Eco130I CCWWGG 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 292
EcoT22I ATGCAT 2 cut(s) 164, 490
EgeI GGCGCC 1 cut(s) 500
EheI GGCGCC 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 4 cut(s) 166, 441, 546, 578
FatI CATG 4 cut(s) 162, 437, 542, 574
FauNDI CATATG 1 cut(s) 158
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 54, 312
FokI GGATG 3 cut(s) 369, 381, 507
Fsp4HI GCNGC 2 cut(s) 54, 312
FspBI CTAG 2 cut(s) 237, 530
GlaI GCGC 1 cut(s) 500
GluI GCNGC 2 cut(s) 54, 312
HaeII RGCGCY 1 cut(s) 502
HhaI GCGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 499
Hin1II CATG 4 cut(s) 166, 441, 546, 578
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HinfI GANTC 1 cut(s) 554
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 551, 575
Hpy8I GTNNAC 1 cut(s) 123
HpyAV CCTTC 3 cut(s) 51, 163, 443
HpyCH4III ACNGT 3 cut(s) 104, 301, 537
HpyCH4V TGCA 6 cut(s) 9, 53, 162, 314, 351, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 499
Hsp92II CATG 4 cut(s) 166, 441, 546, 578
HspAI GCGC 1 cut(s) 499
KasI GGCGCC 1 cut(s) 498
Kzo9I GATC 3 cut(s) 107, 325, 582
LguI GCTCTTC 1 cut(s) 170
LpnPI CCDG 8 cut(s) 60, 63, 87, 131, 201, 224, 405, 536
Lsp1109I GCAGC 2 cut(s) 65, 298
MaeI CTAG 2 cut(s) 237, 530
MaeIII GTNAC 1 cut(s) 98
MalI GATC 3 cut(s) 109, 327, 584
MboI GATC 3 cut(s) 107, 325, 582
MboII GAAGA 5 cut(s) 146, 187, 261, 590, 602
MluCI AATT 7 cut(s) 196, 223, 316, 362, 376, 446, 601
Mly113I GGCGCC 1 cut(s) 499
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 4 cut(s) 25, 212, 296, 417
Mph1103I ATGCAT 2 cut(s) 164, 490
MseI TTAA 2 cut(s) 116, 242
MslI CAYNNNNRTG 3 cut(s) 161, 351, 493
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 45
NarI GGCGCC 1 cut(s) 499
NdeI CATATG 1 cut(s) 158
NdeII GATC 3 cut(s) 107, 325, 582
NlaIII CATG 4 cut(s) 166, 441, 546, 578
NlaIV GGNNCC 1 cut(s) 500
NsiI ATGCAT 2 cut(s) 164, 490
NspV TTCGAA 1 cut(s) 218
PagI TCATGA 1 cut(s) 574
PciSI GCTCTTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 2 cut(s) 55, 313
PleI GAGTC 1 cut(s) 548
PluTI GGCGCC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 548
PshBI ATTAAT 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 500
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 151
RseI CAYNNNNRTG 3 cut(s) 161, 351, 493
SapI GCTCTTC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 116, 242
SatI GCNGC 2 cut(s) 54, 312
Sau3AI GATC 3 cut(s) 107, 325, 582
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 1 cut(s) 75
SfcI CTRYAG 2 cut(s) 51, 103
SfoI GGCGCC 1 cut(s) 500
SfuI TTCGAA 1 cut(s) 218
SmiMI CAYNNNNRTG 3 cut(s) 161, 351, 493
Sse9I AATT 7 cut(s) 196, 223, 316, 362, 376, 446, 601
SspDI GGCGCC 1 cut(s) 498
SspI AATATT 1 cut(s) 114
SspMI CTAG 2 cut(s) 237, 530
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 3 cut(s) 104, 301, 537
TaqI TCGA 3 cut(s) 204, 218, 613
TasI AATT 7 cut(s) 196, 223, 316, 362, 376, 446, 601
Tru1I TTAA 2 cut(s) 116, 242
Tru9I TTAA 2 cut(s) 116, 242
TscAI CASTG 1 cut(s) 423
TseI GCWGC 2 cut(s) 53, 311
TspDTI ATGAA 5 cut(s) 250, 322, 531, 563, 591
TspRI CASTG 1 cut(s) 423
Van91I CCANNNNNTGG 1 cut(s) 466
VspI ATTAAT 1 cut(s) 116
XapI RAATTY 2 cut(s) 362, 446
XmiI GTMKAC 1 cut(s) 122
XspI CTAG 2 cut(s) 237, 530
Zsp2I ATGCAT 2 cut(s) 164, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.