MD12G1146100.v1.1

Belongs to the cyclin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010288
Physical Location & Seq
Reverse (-)
22499258 .. 22501212
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1146100.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGTTTGCATGGAAAGTTTGCTTTGTGATGAGGTATGCCCTTCAAGCCCCCCTGCAGTGACTACTGAGCCTTTCGGTCACAGTAACTTTGGATCCTCAATGTATACAACTAAGGAAGACTATGAGCAGGCTCTTACCATATGCATGGAGAAGGAGATCAGCTACATGCCTGAACCTAATTATCTAGAGAACCTATCTTCCAATAATTTGGTCATTGCTAGGTTAAAATGCATTCAGTGGTTCATCAAGTCTGCAGGTCGGTTGAACCTCTCCCTTGGAACCGTTTTCTATGCTGCAAATTACCTTGATCGGTTCATATCCATGAACCATTGCACTGGATGGAAATATTGGATGGTTGAATTGGTGTCTGTTGCCTGCTTATCTATTGCCAACAAGTTCAGTGATACCTGCAATCCTTCCCTGCTTGAACTTCAGATGGAAGATCTGGACCATTCATTTGAGCCAAGCACAATCCAACGAATGGAAATGATGGTGCTAAATGCATTAGGATGGCGCCTTGCGTCTACAACAGCTTACTCTTACCTAGAACTGTTGGAATGGATTCTGGACTCTTTGAAGCCCCAACTTCATGAAAAGCTCATAGGCCGACTTAACAAATTGCTTCTTGGAGCTATTTCAGATTCCAAGCTTCTGGATTTTAGGCCAAGTGTGGTTGCAATATCTGCTCTAAGGTGCAGTCTGGATAAGATACAACTTTCAGAAGCCATATCTGATAACGCCTACCTCACGTGTCTTACAAGTCTATTGGATCATGACCAAAAGTGTGAGTTGGTGAAGTGCCATATGATGATGGAGGAGCAATATTCATCAGTTGATTGTTTGGACAACTTAAGAGCTCACCGGGATTTTCATTACTGCCCTTCAAGTCCCACGACCGTGCTGTTGAAGAATCGTATTAATTTTTATGATTTTCATGTTGATTTCTCTCTCTTTAAGATGAGAGATCAAACAATGATCACCGGGATTAACAAGAGAAAGCGAGAAGATCACTACCCTAATTAA

Protein Analysis

341

Amino Acids

39.06

Weight (kDa)

5.4

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 47 - 173 2.7e-21 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 245, 416
AccB1I GGYRCC 1 cut(s) 513
AccB7I CCANNNNNTGG 1 cut(s) 481
AccI GTMKAC 2 cut(s) 104, 524
AclWI GGATC 3 cut(s) 87, 100, 777
AcuI CTGAAG 1 cut(s) 416
AcvI CACGTG 1 cut(s) 750
AcyI GRCGYC 1 cut(s) 514
AfiI CCNNNNNNNGG 1 cut(s) 481
AflII CTTAAG 1 cut(s) 850
AflIII ACRYGT 1 cut(s) 749
AgsI TTSAA 7 cut(s) 45, 265, 359, 428, 577, 885, 907
AleI CACNNNNGTG 1 cut(s) 896
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 6 cut(s) 162, 533, 598, 632, 649, 857
AluI AGCT 6 cut(s) 162, 533, 598, 632, 649, 857
Alw21I GWGCWC 1 cut(s) 859
AlwI GGATC 3 cut(s) 87, 100, 777
AoxI GGCC 2 cut(s) 604, 662
ApeKI GCWGC 1 cut(s) 293
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 2 cut(s) 863, 982
AsuHPI GGTGA 3 cut(s) 805, 851, 970
AvaII GGWCC 1 cut(s) 448
BamHI GGATCC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 513
BanII GRGCYC 1 cut(s) 859
BbrPI CACGTG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 1 cut(s) 280
BccI CCATC 6 cut(s) 333, 346, 430, 484, 504, 805
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 2 cut(s) 863, 982
BfaI CTAG 3 cut(s) 185, 219, 545
BfmI CTRYAG 2 cut(s) 54, 252
BfoI RGCGCY 1 cut(s) 517
BfrI CTTAAG 1 cut(s) 850
BfuAI ACCTGC 2 cut(s) 245, 416
BglII AGATCT 1 cut(s) 442
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
Bme1390I CCNGG 2 cut(s) 863, 982
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 3 cut(s) 94, 280, 515
BmrFI CCNGG 2 cut(s) 863, 982
BpiI GAAGAC 1 cut(s) 123
BpuMI CCSGG 2 cut(s) 863, 982
BsaAI YACGTR 1 cut(s) 750
BsaHI GRCGYC 1 cut(s) 514
BsaJI CCNNGG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 2 cut(s) 213, 328
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 3 cut(s) 344, 357, 515
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMI GCAATG 2 cut(s) 213, 328
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 830
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 715
Bsh1285I CGRYCG 1 cut(s) 897
BshFI GGCC 2 cut(s) 606, 664
BshNI GGYRCC 1 cut(s) 513
BsiEI CGRYCG 1 cut(s) 897
BsiHKAI GWGCWC 1 cut(s) 859
BsiSI CCGG 2 cut(s) 862, 981
BslFI GGGAC 1 cut(s) 873
BslI CCNNNNNNNGG 1 cut(s) 481
BsmFI GGGAC 1 cut(s) 873
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 2 cut(s) 606, 664
Bsp1286I GDGCHC 1 cut(s) 859
Bsp143I GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
BspANI GGCC 2 cut(s) 606, 664
BspCNI CTCAG 1 cut(s) 58
BspHI TCATGA 2 cut(s) 589, 772
BspLI GGNNCC 3 cut(s) 94, 280, 515
BspMAI CTGCAG 2 cut(s) 58, 256
BspMI ACCTGC 2 cut(s) 245, 416
BspPI GGATC 3 cut(s) 87, 100, 777
BspT107I GGYRCC 1 cut(s) 513
BspTI CTTAAG 1 cut(s) 850
BsrDI GCAATG 2 cut(s) 213, 328
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
BssNAI GTATAC 1 cut(s) 105
BssNI GRCGYC 1 cut(s) 514
BssT1I CCWWGG 1 cut(s) 274
Bst1107I GTATAC 1 cut(s) 105
Bst4CI ACNGT 4 cut(s) 83, 283, 552, 898
BstACI GRCGYC 1 cut(s) 514
BstAFI CTTAAG 1 cut(s) 850
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 750
BstC8I GCNNGC 2 cut(s) 129, 376
BstDEI CTNAG 3 cut(s) 66, 111, 689
BstF5I GGATG 3 cut(s) 344, 357, 515
BstH2I RGCGCY 1 cut(s) 517
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 8 cut(s) 95, 159, 310, 445, 772, 967, 978, 1009
BstMBI GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
BstMCI CGRYCG 1 cut(s) 897
BstMWI GCNNNNNNNGC 2 cut(s) 45, 683
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 861, 980
BstSFI CTRYAG 2 cut(s) 54, 252
BstV1I GCAGC 1 cut(s) 280
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 92, 442
BstXI CCANNNNNNTGG 4 cut(s) 145, 208, 335, 652
BstYI RGATCY 2 cut(s) 92, 442
BstZ17I GTATAC 1 cut(s) 105
BsuRI GGCC 2 cut(s) 606, 664
BtsCI GGATG 3 cut(s) 344, 357, 515
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 4 cut(s) 63, 242, 333, 406
BveI ACCTGC 2 cut(s) 245, 416
Cac8I GCNNGC 2 cut(s) 129, 376
CciI TCATGA 2 cut(s) 589, 772
CfoI GCGC 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 448
CseI GACGC 1 cut(s) 510
CviAII CATG 7 cut(s) 10, 145, 166, 322, 590, 773, 935
DdeI CTNAG 3 cut(s) 66, 111, 689
DinI GGCGCC 1 cut(s) 515
DpnI GATC 8 cut(s) 94, 158, 309, 444, 771, 966, 977, 1008
DpnII GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
Ecl136II GAGCTC 1 cut(s) 857
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 416
Eco72I CACGTG 1 cut(s) 750
EcoICRI GAGCTC 1 cut(s) 857
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 3 cut(s) 146, 233, 505
EcoT38I GRGCYC 1 cut(s) 859
EgeI GGCGCC 1 cut(s) 515
EheI GGCGCC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 7 cut(s) 13, 148, 169, 325, 593, 776, 938
FaqI GGGAC 1 cut(s) 873
FatI CATG 7 cut(s) 9, 144, 165, 321, 589, 772, 934
FauNDI CATATG 2 cut(s) 140, 804
FbaI TGATCA 1 cut(s) 975
FblI GTMKAC 2 cut(s) 104, 524
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 3 cut(s) 351, 364, 522
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 3 cut(s) 185, 219, 545
GlaI GCGC 1 cut(s) 515
GluI GCNGC 1 cut(s) 294
HaeII RGCGCY 1 cut(s) 517
HaeIII GGCC 2 cut(s) 606, 664
HapII CCGG 2 cut(s) 862, 981
HgaI GACGC 1 cut(s) 510
HhaI GCGC 1 cut(s) 516
Hin1I GRCGYC 1 cut(s) 514
Hin1II CATG 7 cut(s) 13, 148, 169, 325, 593, 776, 938
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 4 cut(s) 562, 569, 641, 910
HpaII CCGG 2 cut(s) 862, 981
HphI GGTGA 3 cut(s) 805, 851, 970
Hpy166II GTNNAC 2 cut(s) 105, 525
Hpy188I TCNGA 4 cut(s) 435, 640, 721, 733
Hpy188III TCNNGA 7 cut(s) 185, 446, 566, 590, 653, 701, 773
Hpy8I GTNNAC 2 cut(s) 105, 525
HpyAV CCTTC 4 cut(s) 51, 145, 426, 891
HpyCH4III ACNGT 4 cut(s) 83, 283, 552, 898
HpyCH4IV ACGT 1 cut(s) 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 683
HpyF3I CTNAG 3 cut(s) 66, 111, 689
HpySE526I ACGT 1 cut(s) 749
Hsp92I GRCGYC 1 cut(s) 514
Hsp92II CATG 7 cut(s) 13, 148, 169, 325, 593, 776, 938
HspAI GCGC 1 cut(s) 514
KasI GGCGCC 1 cut(s) 513
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
LmnI GCTCC 2 cut(s) 629, 817
Lsp1109I GCAGC 1 cut(s) 280
MaeI CTAG 3 cut(s) 185, 219, 545
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 3 cut(s) 58, 77, 83
MalI GATC 8 cut(s) 94, 158, 309, 444, 771, 966, 977, 1008
MboI GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
MboII GAAGA 5 cut(s) 128, 189, 452, 919, 1016
MflI RGATCY 2 cut(s) 92, 442
MhlI GDGCHC 1 cut(s) 859
MluCI AATT 7 cut(s) 178, 205, 298, 359, 617, 919, 1018
Mly113I GGCGCC 1 cut(s) 514
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 499, 534
MnlI CCTC 5 cut(s) 25, 106, 278, 755, 808
Mph1103I ATGCAT 3 cut(s) 146, 233, 505
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 7 cut(s) 224, 612, 851, 918, 954, 987, 1021
MslI CAYNNNNRTG 4 cut(s) 143, 320, 508, 896
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 2 cut(s) 862, 981
MspR9I CCNGG 2 cut(s) 863, 982
Mva1269I GAATGC 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 45, 683
NarI GGCGCC 1 cut(s) 514
NciI CCSGG 2 cut(s) 863, 982
NdeI CATATG 2 cut(s) 140, 804
NdeII GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
NlaIII CATG 7 cut(s) 13, 148, 169, 325, 593, 776, 938
NlaIV GGNNCC 3 cut(s) 94, 280, 515
NmuCI GTSAC 2 cut(s) 58, 77
NsiI ATGCAT 3 cut(s) 146, 233, 505
NspI RCATGY 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 896
PagI TCATGA 2 cut(s) 589, 772
PctI GAATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 562, 641, 910
PflMI CCANNNNNTGG 1 cut(s) 481
PkrI GCNGC 1 cut(s) 295
PleI GAGTC 1 cut(s) 563
PluTI GGCGCC 1 cut(s) 517
PmaCI CACGTG 1 cut(s) 750
PmlI CACGTG 1 cut(s) 750
PpsI GAGTC 1 cut(s) 563
Ppu21I YACGTR 1 cut(s) 750
PshBI ATTAAT 1 cut(s) 918
Psp124BI GAGCTC 1 cut(s) 859
PspCI CACGTG 1 cut(s) 750
PspN4I GGNNCC 3 cut(s) 94, 280, 515
PspPI GGNCC 1 cut(s) 448
PstI CTGCAG 2 cut(s) 58, 256
PsuI RGATCY 2 cut(s) 92, 442
RseI CAYNNNNRTG 4 cut(s) 143, 320, 508, 896
SacI GAGCTC 1 cut(s) 859
SaqAI TTAA 7 cut(s) 224, 612, 851, 918, 954, 987, 1021
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 8 cut(s) 92, 156, 307, 442, 769, 964, 975, 1006
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 863, 982
SduI GDGCHC 1 cut(s) 859
SfcI CTRYAG 2 cut(s) 54, 252
SfoI GGCGCC 1 cut(s) 515
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 4 cut(s) 143, 320, 508, 896
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 7 cut(s) 178, 205, 298, 359, 617, 919, 1018
SspDI GGCGCC 1 cut(s) 513
SspI AATATT 2 cut(s) 347, 824
SspMI CTAG 3 cut(s) 185, 219, 545
SstI GAGCTC 1 cut(s) 859
StyD4I CCNGG 2 cut(s) 861, 980
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 4 cut(s) 83, 283, 552, 898
TaiI ACGT 1 cut(s) 752
TaqII GACCGA 1 cut(s) 65
TasI AATT 7 cut(s) 178, 205, 298, 359, 617, 919, 1018
TfiI GAWTC 3 cut(s) 562, 641, 910
Tru1I TTAA 7 cut(s) 224, 612, 851, 918, 954, 987, 1021
Tru9I TTAA 7 cut(s) 224, 612, 851, 918, 954, 987, 1021
TscAI CASTG 4 cut(s) 63, 242, 340, 406
TseFI GTSAC 2 cut(s) 58, 77
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 2 cut(s) 58, 77
TspDTI ATGAA 9 cut(s) 232, 304, 338, 444, 578, 606, 816, 860, 923
TspRI CASTG 4 cut(s) 63, 242, 340, 406
Van91I CCANNNNNTGG 1 cut(s) 481
Vha464I CTTAAG 1 cut(s) 850
VpaK11BI GGWCC 1 cut(s) 448
VspI ATTAAT 1 cut(s) 918
XbaI TCTAGA 1 cut(s) 184
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 104, 524
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 3 cut(s) 185, 219, 545
Zsp2I ATGCAT 3 cut(s) 146, 233, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.