Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003135

Physical Location & Seq

Genomic Coordinates Reverse (-)

23662380 .. 23665820

Gene Structure 3441 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1148200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGGACGTCATCAAAACGCAGCAAGTTTCAGCGAGACTGATCGAGAAGGTGATAGTTCACCCGCTCGTTCTGCTCAGCATCGTAGACAACTACAACCGAGTCGCCAAGGACACTCGCAAGCGGGTAGTTGGCGTTCTGCTCGGAAGCACCTACAAAGGTGTCGTCGACGTCTCCAACAGCTACGCAGTGCCCTTCGAAGAAGATGAGAAAGACCCTAGCATTTGGTTTCTTGACCATAACTACCATGAAGCAATGTTCTCCATGTTCAAGAGGATCAATGCCAAAGAGCACGTTGTAGGTTGGTATAGCACCGGGCCAAAACTTAAAGAGAATGATCTGGATATTCACAGTTTATTCCACAATTATGTCCCAAATCCAGTCCTGGTCATTATTGATGTTCAGCCTAAGGAGTTGGGAATACCCACCAAAGCCTACTATGATGTCGAAGAAGTTAAAGAGAATGCAACTCAAAAAAGCCAGAAGATTTTTGTTCATGTGCCCTCTGAAATTGCTGCTCATGAGGTTGAGGAAATCGGAGTGGAACACTTACTAAGAGATGTAAAGGATACAACCATCAGCACACTTGCAAATGAGGTTACTGGAAAACTTACTGCTCTAAAGGGTTTGGATGCACGGTTACGAGAAATACGCAGTTACCTAGATCTTGTCATTGATGAGAAGCTTCCACTTAATCATGAGATACTTTACCATTTACAGGACGTGTTCAATCTACTTCCAAATCTCAATGTGGCAGAATTAGTGAAGGGATTTTCAGTAAAAACAAATGACATGATGTTGGTTATATATCTTTCTTCTCTCATCCGAAGCGTCATCGCCCTCCACAACTTGATCAACAACAAGATGCTCAACAAAGAACATGAGAAAGCTGAGGATGCAAAGCCGACAACTGCCCCGTCTGTCGCTGGAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000502 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010498 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0032501 GO:0032502 GO:0032991 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051603 GO:0061458 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 ko05169 map03050 map05169

Pfam Domains

Protein Families

JAB MitMem_reg

Protein Analysis

311

Amino Acids

35.07

Weight (kDa)

5.91

Isoelectric Point (pI)

33.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JAB	PF01398	15 - 125	3.7e-35	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
MitMem_reg	PF13012	174 - 285	3.8e-39	Maintenance of mitochondrial structure and function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11270 AT3G11270 AT5G05780 AT5G05780
fragaria_vesca	FvH4_6g11900 FvH4_7g24080
malus_domestica	MD01G1147900.v1.1 MD04G1148200.v1.1 MD07G1215000.v1.1 MD12G1161700.v1.1
prunus_persica	Prupe.2G247000_v2.0.a1 Prupe.2G247000_v2.0.a1 Prupe.6G272100_v2.0.a1
pyrus_communis	pycom01g16670 pycom04g13410 pycom07g19730 pycom12g15350
rosa_chinensis	RchiOBHm_Chr1g0369511 RchiOBHm_Chr3g0463641
rosa_laevigata	RLG00000024766 RLG00000027123
rosa_multiflora	Rmu_sc0003267.1_g000028 Rmu_ssc0000484.1_g000026
rosa_roxburghii	Rroxscaffold_4G00287570 Rroxscaffold_6G00416390
rosa_rugosa	Rorug01G0351000 Rorug03G0066700
rosa_samantha	Rh1AG359100 Rh1BG322800 Rh1CG336100 Rh1DG353600 Rh3AG123700 Rh3BG128400 Rh3CG130600 Rh3DG129000
rosa_wichuraiana	Rw1G031620 Rw3G010460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	9, 171
AccBSI	CCGCTC	1 cut(s)	64
AccI	GTMKAC	2 cut(s)	84, 165
AciI	CCGC	2 cut(s)	62, 121
AclWI	GGATC	1 cut(s)	281
AcyI	GRCGYC	2 cut(s)	6, 168
AfiI	CCNNNNNNNGG	1 cut(s)	715
AflIII	ACRYGT	1 cut(s)	720
AgsI	TTSAA	2 cut(s)	268, 727
AjiI	CACGTC	1 cut(s)	721
AjnI	CCWGG	1 cut(s)	381
AluBI	AGCT	4 cut(s)	180, 682, 887, 930
AluI	AGCT	4 cut(s)	180, 682, 887, 930
Alw21I	GWGCWC	1 cut(s)	291
Alw26I	GTCTC	2 cut(s)	28, 175
AlwI	GGATC	1 cut(s)	281
AoxI	GGCC	1 cut(s)	314
ApeKI	GCWGC	2 cut(s)	19, 512
ArsI	GACNNNNNNTTYG	2 cut(s)	147, 179
AspS9I	GGNCC	1 cut(s)	314
AsuC2I	CCSGG	1 cut(s)	313
AsuHPI	GGTGA	2 cut(s)	50, 61
AsuII	TTCGAA	1 cut(s)	195
AxyI	CCTNAGG	1 cut(s)	405
BaeGI	GKGCMC	2 cut(s)	192, 501
Bbv12I	GWGCWC	1 cut(s)	291
BbvCI	CCTCAGC	1 cut(s)	888
BbvI	GCAGC	2 cut(s)	31, 499
BccI	CCATC	1 cut(s)	581
BciT130I	CCWGG	1 cut(s)	383
BciVI	GTATCC	1 cut(s)	559
BclI	TGATCA	1 cut(s)	849
BcnI	CCSGG	1 cut(s)	313
BcoDI	GTCTC	2 cut(s)	28, 175
BfaI	CTAG	2 cut(s)	216, 659
BfuI	GTATCC	1 cut(s)	559
BglII	AGATCT	1 cut(s)	661
BisI	GCNGC	2 cut(s)	20, 513
BlpI	GCTNAGC	1 cut(s)	74
BlsI	GCNGC	2 cut(s)	21, 514
Bme1390I	CCNGG	2 cut(s)	313, 383
BmgBI	CACGTC	1 cut(s)	721
BmgT120I	GGNCC	1 cut(s)	314
BmrFI	CCNGG	2 cut(s)	313, 383
BmsI	GCATC	4 cut(s)	87, 619, 852, 883
Bpu10I	CCTNAGC	1 cut(s)	888
Bpu1102I	GCTNAGC	1 cut(s)	74
Bpu14I	TTCGAA	1 cut(s)	195
BpuMI	CCSGG	1 cut(s)	313
BsaHI	GRCGYC	2 cut(s)	6, 168
BsaJI	CCNNGG	1 cut(s)	105
Bsc4I	CCNNNNNNNGG	1 cut(s)	715
Bse1I	ACTGG	2 cut(s)	377, 604
Bse21I	CCTNAGG	1 cut(s)	405
Bse3DI	GCAATG	1 cut(s)	258
BseBI	CCWGG	1 cut(s)	383
BseDI	CCNNGG	1 cut(s)	105
BseGI	GGATG	3 cut(s)	634, 819, 898
BseLI	CCNNNNNNNGG	1 cut(s)	715
BseMI	GCAATG	1 cut(s)	258
BseMII	CTCAG	2 cut(s)	88, 879
BseNI	ACTGG	2 cut(s)	377, 604
BseSI	GKGCMC	2 cut(s)	192, 501
BseXI	GCAGC	2 cut(s)	31, 499
BshFI	GGCC	1 cut(s)	316
BsiHKAI	GWGCWC	1 cut(s)	291
BsiSI	CCGG	1 cut(s)	312
BslFI	GGGAC	1 cut(s)	353
BslI	CCNNNNNNNGG	1 cut(s)	715
BsmAI	GTCTC	2 cut(s)	28, 175
BsmBI	CGTCTC	1 cut(s)	175
BsmFI	GGGAC	1 cut(s)	353
BsmI	GAATGC	1 cut(s)	466
BsnI	GGCC	1 cut(s)	316
Bsp119I	TTCGAA	1 cut(s)	195
Bsp1286I	GDGCHC	3 cut(s)	192, 291, 501
Bsp143I	GATC	5 cut(s)	39, 273, 334, 661, 849
Bsp1720I	GCTNAGC	1 cut(s)	74
BspACI	CCGC	2 cut(s)	62, 121
BspANI	GGCC	1 cut(s)	316
BspCNI	CTCAG	2 cut(s)	87, 880
BspHI	TCATGA	2 cut(s)	517, 694
BspPI	GGATC	1 cut(s)	281
BspT104I	TTCGAA	1 cut(s)	195
BsrBI	CCGCTC	1 cut(s)	64
BsrDI	GCAATG	1 cut(s)	258
BsrI	ACTGG	2 cut(s)	377, 604
BssECI	CCNNGG	1 cut(s)	105
BssMI	GATC	5 cut(s)	39, 273, 334, 661, 849
BssNI	GRCGYC	2 cut(s)	6, 168
BssT1I	CCWWGG	1 cut(s)	105
Bst2UI	CCWGG	1 cut(s)	383
Bst4CI	ACNGT	2 cut(s)	350, 636
BstACI	GRCGYC	2 cut(s)	6, 168
BstBI	TTCGAA	1 cut(s)	195
BstC8I	GCNNGC	1 cut(s)	119
BstDEI	CTNAG	4 cut(s)	74, 405, 551, 888
BstF5I	GGATG	3 cut(s)	634, 819, 898
BstKTI	GATC	5 cut(s)	42, 276, 337, 664, 852
BstMAI	GTCTC	2 cut(s)	28, 175
BstMBI	GATC	5 cut(s)	39, 273, 334, 661, 849
BstMWI	GCNNNNNNNGC	2 cut(s)	70, 893
BstNI	CCWGG	1 cut(s)	383
BstSCI	CCNGG	2 cut(s)	311, 381
BstSLI	GKGCMC	2 cut(s)	192, 501
BstV1I	GCAGC	2 cut(s)	31, 499
BstX2I	RGATCY	1 cut(s)	661
BstYI	RGATCY	1 cut(s)	661
Bsu36I	CCTNAGG	1 cut(s)	405
BsuI	GTATCC	1 cut(s)	559
BsuRI	GGCC	1 cut(s)	316
BtgZI	GCGATG	1 cut(s)	817
BtrI	CACGTC	1 cut(s)	721
BtsCI	GGATG	3 cut(s)	634, 819, 898
BtsI	GCAGTG	1 cut(s)	192
BtsIMutI	CAGTG	1 cut(s)	192
Cac8I	GCNNGC	1 cut(s)	119
CciI	TCATGA	2 cut(s)	517, 694
Cfr13I	GGNCC	1 cut(s)	314
CseI	GACGC	1 cut(s)	817
CviAII	CATG	7 cut(s)	245, 262, 494, 518, 695, 790, 878
CviJI	RGCY	9 cut(s)	180, 316, 403, 431, 477, 682, 887, 901, 930
CviKI_1	RGCY	9 cut(s)	180, 316, 403, 431, 477, 682, 887, 901, 930
DdeI	CTNAG	4 cut(s)	74, 405, 551, 888
DpnI	GATC	5 cut(s)	41, 275, 336, 663, 851
DpnII	GATC	5 cut(s)	39, 273, 334, 661, 849
Eco130I	CCWWGG	1 cut(s)	105
Eco81I	CCTNAGG	1 cut(s)	405
EcoRII	CCWGG	1 cut(s)	381
EcoT14I	CCWWGG	1 cut(s)	105
ErhI	CCWWGG	1 cut(s)	105
Esp3I	CGTCTC	1 cut(s)	175
FaeI	CATG	7 cut(s)	248, 265, 497, 521, 698, 793, 881
FaqI	GGGAC	1 cut(s)	353
FatI	CATG	7 cut(s)	244, 261, 493, 517, 694, 789, 877
FauI	CCCGC	2 cut(s)	69, 114
FbaI	TGATCA	1 cut(s)	849
FblI	GTMKAC	2 cut(s)	84, 165
Fnu4HI	GCNGC	2 cut(s)	20, 513
FokI	GGATG	3 cut(s)	641, 806, 905
Fsp4HI	GCNGC	2 cut(s)	20, 513
FspBI	CTAG	2 cut(s)	216, 659
GluI	GCNGC	2 cut(s)	20, 513
HaeIII	GGCC	1 cut(s)	316
HapII	CCGG	1 cut(s)	312
HgaI	GACGC	1 cut(s)	817
Hin1I	GRCGYC	2 cut(s)	6, 168
Hin1II	CATG	7 cut(s)	248, 265, 497, 521, 698, 793, 881
HincII	GTYRAC	1 cut(s)	166
HindII	GTYRAC	1 cut(s)	166
HindIII	AAGCTT	1 cut(s)	680
HinfI	GANTC	1 cut(s)	99
HpaII	CCGG	1 cut(s)	312
HphI	GGTGA	2 cut(s)	50, 61
Hpy166II	GTNNAC	3 cut(s)	58, 85, 166
Hpy188I	TCNGA	4 cut(s)	143, 505, 536, 824
Hpy188III	TCNNGA	6 cut(s)	43, 230, 268, 338, 518, 695
Hpy8I	GTNNAC	3 cut(s)	58, 85, 166
Hpy99I	CGWCG	2 cut(s)	167, 170
HpyAV	CCTTC	3 cut(s)	40, 202, 757
HpyCH4III	ACNGT	2 cut(s)	350, 636
HpyCH4IV	ACGT	4 cut(s)	6, 168, 291, 720
HpyCH4V	TGCA	4 cut(s)	464, 587, 632, 896
HpyF10VI	GCNNNNNNNGC	2 cut(s)	70, 893
HpyF3I	CTNAG	4 cut(s)	74, 405, 551, 888
HpySE526I	ACGT	4 cut(s)	6, 168, 291, 720
Hsp92I	GRCGYC	2 cut(s)	6, 168
Hsp92II	CATG	7 cut(s)	248, 265, 497, 521, 698, 793, 881
Ksp22I	TGATCA	1 cut(s)	849
Kzo9I	GATC	5 cut(s)	39, 273, 334, 661, 849
LpnPI	CCDG	9 cut(s)	323, 325, 368, 390, 395, 491, 585, 701, 909
Lsp1109I	GCAGC	2 cut(s)	31, 499
LweI	GCATC	4 cut(s)	87, 619, 852, 883
MaeI	CTAG	2 cut(s)	216, 659
MaeII	ACGT	4 cut(s)	6, 168, 291, 720
MaeIII	GTNAC	3 cut(s)	595, 636, 653
MalI	GATC	5 cut(s)	41, 275, 336, 663, 851
MbiI	CCGCTC	1 cut(s)	64
MboI	GATC	5 cut(s)	39, 273, 334, 661, 849
MboII	GAAGA	5 cut(s)	209, 212, 458, 493, 804
MflI	RGATCY	1 cut(s)	661
MhlI	GDGCHC	3 cut(s)	192, 291, 501
MluCI	AATT	3 cut(s)	361, 507, 755
MlyI	GAGTC	1 cut(s)	108
MmeI	TCCRAC	1 cut(s)	198
MnlI	CCTC	7 cut(s)	264, 511, 514, 520, 586, 848, 883
MseI	TTAA	3 cut(s)	324, 453, 690
MslI	CAYNNNNRTG	1 cut(s)	363
MspI	CCGG	1 cut(s)	312
MspR9I	CCNGG	2 cut(s)	313, 383
Mva1269I	GAATGC	1 cut(s)	466
MvaI	CCWGG	1 cut(s)	383
MwoI	GCNNNNNNNGC	2 cut(s)	70, 893
NciI	CCSGG	1 cut(s)	313
NdeII	GATC	5 cut(s)	39, 273, 334, 661, 849
NlaIII	CATG	7 cut(s)	248, 265, 497, 521, 698, 793, 881
NspV	TTCGAA	1 cut(s)	195
PagI	TCATGA	2 cut(s)	517, 694
PctI	GAATGC	1 cut(s)	466
PkrI	GCNGC	2 cut(s)	21, 514
PleI	GAGTC	1 cut(s)	107
PpsI	GAGTC	1 cut(s)	107
Psp6I	CCWGG	1 cut(s)	381
PspGI	CCWGG	1 cut(s)	381
PspPI	GGNCC	1 cut(s)	314
PsrI	GAACNNNNNNTAC	2 cut(s)	117, 149
PsuI	RGATCY	1 cut(s)	661
RseI	CAYNNNNRTG	1 cut(s)	363
SalI	GTCGAC	1 cut(s)	164
SaqAI	TTAA	3 cut(s)	324, 453, 690
SatI	GCNGC	2 cut(s)	20, 513
Sau3AI	GATC	5 cut(s)	39, 273, 334, 661, 849
Sau96I	GGNCC	1 cut(s)	314
SchI	GAGTC	1 cut(s)	108
ScrFI	CCNGG	2 cut(s)	313, 383
SduI	GDGCHC	3 cut(s)	192, 291, 501
SfaNI	GCATC	4 cut(s)	87, 619, 852, 883
SfuI	TTCGAA	1 cut(s)	195
SgrDI	CGTCGACG	1 cut(s)	164
SmiMI	CAYNNNNRTG	1 cut(s)	363
Sse9I	AATT	3 cut(s)	361, 507, 755
SsiI	CCGC	2 cut(s)	62, 121
SspMI	CTAG	2 cut(s)	216, 659
StyD4I	CCNGG	2 cut(s)	311, 381
StyI	CCWWGG	1 cut(s)	105
TaaI	ACNGT	2 cut(s)	350, 636
TaiI	ACGT	4 cut(s)	9, 171, 294, 723
TaqI	TCGA	4 cut(s)	42, 165, 195, 444
TasI	AATT	3 cut(s)	361, 507, 755
Tru1I	TTAA	3 cut(s)	324, 453, 690
Tru9I	TTAA	3 cut(s)	324, 453, 690
TscAI	CASTG	1 cut(s)	192
TseI	GCWGC	2 cut(s)	19, 512
TspDTI	ATGAA	2 cut(s)	261, 482
TspRI	CASTG	1 cut(s)	192
XmiI	GTMKAC	2 cut(s)	84, 165
XspI	CTAG	2 cut(s)	216, 659
ZraI	GACGTC	2 cut(s)	7, 169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.