Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009476

Physical Location & Seq

Genomic Coordinates Forward (+)

26929819 .. 26932730

Gene Structure 2912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1178400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGAGACGTGCGCCGTGCGTTCACACGCCCTCGATTCGACTTGCGAGTTCACAAACGTCCCCTTCCTTCCCTCATCTCCTTCATTTCTCCAACGACCGAGTGAGTGAGACCTTCGTCCTCCCCAAAACTCACGTTTTCAGAAATTTCACTGCATTTCTCAGATCGGAGCTTTATGTGACGATCGGAGTTTTTCAGATGGATGCAGCCTCACAAAGCTTCTTGGAAGAGTTGCTTCAGTCCGCTGTCGACAATCATGAGGATAGTGGTGTGGTAAATGAACATACTGGGCAGCCTGCAACGTGTGCGCTATGCCAAAGGGCTATTTCACCCGAGAATGAGGCGACTGGAGATGTTGAAATTATGTGCGGGGACTGTAAATTTCTGTACCTTGAAGATCTTGGTTCCCCCTCACATGATTCTTATCAGAGGACGCCACCTACAAGAAGAAGAGCTAGGACTGGGAGTTCCGAGTCACTTGAGAATACTTTTTCACAACAGTTCTCGCATATGATTAATCTGGTGCGGCAGAACCAATCCCCTGGCTCTGGGATTGAGGAACTACCTGTGGGTGGTGACTCTGCGACTAGGATACCGCTGCGCACAAGCAACCGTACTACACCAAGTGGGTCTAGAAGATGGCGGCGAGTGCTCTCTGATACTGAAAGTGAGGGTTACGAAAATGTGGATTCTTTCTATGGAGAAACTGAATCAAATCTCAGTTTTGGTCGGTACAGGGTATTTCATAGTGAGAGCGATGCAATTTCTTTAAGTGCATATGGAGGGGATTCTGATGCTTCTGTGGACCTACATGGTTTACTGGATACTGAAACGATTATTCCACCAGATGATGGAAGTGCTTATGATAGTGATACTGACATTGATCCAATGCATGCTGGTCTTAGCCAGTGGAACTCAGATGACACTGAGGACGATGATGATGAGGGGGATGAAGAAGATGATGAGTGGGAAGAAGCCGACATTGAAGTAAACATGGTTGAATCTAGTGAAACCCCAGGTGGCCGGCTTCGTAATCTTTTAGTTTCGCGTCCAGGCAGAAGTAATGGACCATCCATTGGGCGCCACCATACATATTTTCCCGATTCTGAGGGTACGTTTCATTGGAGAATGAGGAGGAGGAGTCAAATATATACCCGTACCCTCTTTTCTAACGTGGACGAATCAGAGATGCTGCCTTATGATGGAAATTCTGGTGATTATCTTGATGCTGGAGGCTTTGAAGAATTGCTCGAGCATCTTGCTGAGGTTGATAGCTTAAGACGAGGAGCTCCTCCAGCTTCTGCGTCCTTTGTGAATAATCTGCCTCGTGTTGTCATTAGCAAGGAACATGACAAGCATGATGACTTAGCCTGTGCAATTTGCAAAGATGTATTGACAATTGGTACTGAAGTGAATAAGCTTCCTTGCTCTCACCTCTATCACTCTATGTGTATTTTGCCATGGTTGAGTACACGGAATACTTGTCCACTGTGTCGATATGAGCTACCGACTGATGACAGAGACTACGAAGAGGGGAAACGGAACGTTAGTGCTAGAGCAGAGATCCACAATGTCCGGCAGCAGAATGCAAGTGTGGACAGTTCCTCCGGTGCTTTCAACAGAGCTTATGAAGATGAGGAGCATGAGCAGAGTGCAGCAAATAGGGAACCAACCACTAATGGCTCGAGAGGAGAAAATAGTCGGGGGAGATGGTTTCTTCTCGCAGCTGCTCCAATTGTCAGTCTTGTGGGTATTGTTCTTGTGCTGTGGTTAGGAAGTCCGCTGTTGGAAAGAAGAGGTCTGGCAGGAAACCAGAACTTCGCCAATCACGCCCGCCACCAGAGTCCTATCACCGGCGCCTCACCCAACCACAGGGAGAACAGAAGCCGGAGATGGTGGTCACTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2 zf-RING_2

Protein Analysis

636

Amino Acids

70.95

Weight (kDa)

4.97

Isoelectric Point (pI)

60.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	456 - 498	1.2e-11	Ring finger domain
zf-C3HC4_2	PF13923	457 - 497	1.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	457 - 497	7.5e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	457 - 484	7.6e-07	RING-like zinc finger
zf-rbx1	PF12678	468 - 498	6.2e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01980
fragaria_vesca	FvH4_6g09350 FvH4_6g09350 FvH4_6g09350
malus_domestica	MD04G1178400.v1.1 MD12G1193900.v1.1
prunus_persica	Prupe.6G298400_v2.0.a1
pyrus_communis	pycom04g15740 pycom12g18010
rosa_chinensis	RchiOBHm_Chr2g0091181 RchiOBHm_Chr3g0459741
rosa_laevigata	RLG00000016189 RLG00000025061
rosa_multiflora	Rmu_sc0000240.1_g000035
rosa_roxburghii	Rroxscaffold_2G00150420 Rroxscaffold_6G00420010
rosa_rugosa	Rorug02G0014100 Rorug03G0038100
rosa_samantha	Rh3AG096500 Rh3BG099200 Rh3CG101100 Rh3DG100500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	599
AccB1I	GGYRCC	2 cut(s)	1077, 1853
AccB7I	CCANNNNNTGG	2 cut(s)	848, 1073
AccI	GTMKAC	1 cut(s)	246
AccII	CGCG	1 cut(s)	1045
AciI	CCGC	7 cut(s)	240, 366, 523, 593, 640, 1778, 1831
AclI	AACGTT	1 cut(s)	1542
AclWI	GGATC	2 cut(s)	875, 1555
AcoI	YGGCCR	1 cut(s)	1018
AcsI	RAATTY	3 cut(s)	142, 377, 1204
AcuI	CTGAAG	2 cut(s)	218, 1425
AcyI	GRCGYC	3 cut(s)	431, 1078, 1854
AdeI	CACNNNGTG	1 cut(s)	623
AfaI	GTAC	7 cut(s)	386, 613, 731, 1111, 1156, 1402, 1468
AfiI	CCNNNNNNNGG	7 cut(s)	545, 569, 848, 1073, 1199, 1850, 1869
AflII	CTTAAG	1 cut(s)	1273
AflIII	ACRYGT	1 cut(s)	299
AgsI	TTSAA	6 cut(s)	356, 392, 983, 998, 1238, 1615
AjiI	CACGTC	1 cut(s)	8
AjnI	CCWGG	3 cut(s)	538, 1012, 1048
AloI	GAACNNNNNNTCC	2 cut(s)	448, 480
Alw21I	GWGCWC	2 cut(s)	651, 1288
Alw26I	GTCTC	2 cut(s)	101, 1512
AlwI	GGATC	2 cut(s)	875, 1555
AlwNI	CAGNNNCTG	1 cut(s)	1298
Ama87I	CYCGRG	3 cut(s)	329, 1247, 1681
AoxI	GGCC	1 cut(s)	1018
ApeKI	GCWGC	8 cut(s)	203, 289, 595, 1189, 1576, 1652, 1721, 1724
ApoI	RAATTY	3 cut(s)	142, 377, 1204
AseI	ATTAAT	1 cut(s)	513
AspLEI	GCGC	5 cut(s)	13, 307, 600, 1080, 1856
AspS9I	GGNCC	2 cut(s)	802, 1064
AsuHPI	GGTGA	6 cut(s)	318, 584, 1223, 1421, 1840, 1851
AvaI	CYCGRG	3 cut(s)	329, 1247, 1681
AvaII	GGWCC	2 cut(s)	802, 1064
BaeI	ACNNNNGTAYC	2 cut(s)	1392, 1425
BanI	GGYRCC	2 cut(s)	1077, 1853
BanII	GRGCYC	1 cut(s)	1288
BauI	CACGAG	1 cut(s)	1323
Bbv12I	GWGCWC	2 cut(s)	651, 1288
BbvCI	CCTCAGC	1 cut(s)	1260
BbvI	GCAGC	8 cut(s)	215, 301, 582, 1176, 1588, 1664, 1711, 1733
BccI	CCATC	7 cut(s)	190, 630, 842, 1075, 1193, 1701, 1884
BcgI	CGANNNNNNTGC	2 cut(s)	1238, 1272
BciT130I	CCWGG	3 cut(s)	540, 1014, 1050
BciVI	GTATCC	2 cut(s)	582, 814
BcoDI	GTCTC	2 cut(s)	101, 1512
BfaI	CTAG	5 cut(s)	453, 585, 630, 1002, 1551
BfoI	RGCGCY	2 cut(s)	1081, 1857
BfrI	CTTAAG	1 cut(s)	1273
BfuI	GTATCC	2 cut(s)	582, 814
BglII	AGATCT	1 cut(s)	394
Bme1390I	CCNGG	3 cut(s)	540, 1014, 1050
Bme18I	GGWCC	2 cut(s)	802, 1064
BmeT110I	CYCGRG	3 cut(s)	329, 1247, 1681
BmgBI	CACGTC	1 cut(s)	8
BmgT120I	GGNCC	2 cut(s)	802, 1064
BmiI	GGNNCC	4 cut(s)	403, 1079, 1665, 1855
BmrFI	CCNGG	3 cut(s)	540, 1014, 1050
BmrI	ACTGGG	2 cut(s)	294, 468
BmsI	GCATC	6 cut(s)	190, 745, 781, 1176, 1213, 1261
BmuI	ACTGGG	2 cut(s)	294, 468
BoxI	GACNNNNGTC	1 cut(s)	113
BpmI	CTGGAG	3 cut(s)	366, 1248, 1275
Bpu10I	CCTNAGC	1 cut(s)	1260
BpuEI	CTTGAG	1 cut(s)	497
BsaBI	GATNNNNATC	1 cut(s)	420
BsaHI	GRCGYC	3 cut(s)	431, 1078, 1854
BsaI	GGTCTC	1 cut(s)	101
BsaJI	CCNNGG	3 cut(s)	538, 1012, 1457
BsaWI	WCCGGW	1 cut(s)	1604
BsaXI	ACNNNNNCTCC	4 cut(s)	1123, 1153, 1586, 1616
Bsc4I	CCNNNNNNNGG	7 cut(s)	545, 569, 848, 1073, 1199, 1850, 1869
Bse118I	RCCGGY	2 cut(s)	1020, 1850
Bse1I	ACTGG	5 cut(s)	289, 349, 463, 822, 904
Bse8I	GATNNNNATC	1 cut(s)	420
BseBI	CCWGG	3 cut(s)	540, 1014, 1050
BseDI	CCNNGG	3 cut(s)	538, 1012, 1457
BseGI	GGATG	3 cut(s)	205, 952, 1067
BseJI	GATNNNNATC	1 cut(s)	420
BseLI	CCNNNNNNNGG	7 cut(s)	545, 569, 848, 1073, 1199, 1850, 1869
BseMII	CTCAG	6 cut(s)	172, 730, 915, 927, 1095, 1251
BseNI	ACTGG	5 cut(s)	289, 349, 463, 822, 904
BseRI	GAGGAG	7 cut(s)	1144, 1147, 1150, 1278, 1296, 1649, 1701
BseXI	GCAGC	8 cut(s)	215, 301, 582, 1176, 1588, 1664, 1711, 1733
BsgI	GTGCAG	1 cut(s)	1671
Bsh1236I	CGCG	1 cut(s)	1045
Bsh1285I	CGRYCG	2 cut(s)	97, 183
BshFI	GGCC	1 cut(s)	1020
BshNI	GGYRCC	2 cut(s)	1077, 1853
BsiEI	CGRYCG	2 cut(s)	97, 183
BsiHKAI	GWGCWC	2 cut(s)	651, 1288
BsiHKCI	CYCGRG	3 cut(s)	329, 1247, 1681
BsiSI	CCGG	5 cut(s)	1021, 1573, 1605, 1851, 1885
BslFI	GGGAC	2 cut(s)	43, 383
BslI	CCNNNNNNNGG	7 cut(s)	545, 569, 848, 1073, 1199, 1850, 1869
BsmAI	GTCTC	2 cut(s)	101, 1512
BsmFI	GGGAC	2 cut(s)	43, 383
BsmI	GAATGC	1 cut(s)	1588
BsnI	GGCC	1 cut(s)	1020
Bso31I	GGTCTC	1 cut(s)	101
BsoBI	CYCGRG	3 cut(s)	329, 1247, 1681
Bsp1286I	GDGCHC	2 cut(s)	651, 1288
Bsp143I	GATC	5 cut(s)	161, 180, 394, 880, 1560
Bsp19I	CCATGG	1 cut(s)	1457
BspACI	CCGC	7 cut(s)	240, 366, 523, 593, 640, 1778, 1831
BspANI	GGCC	1 cut(s)	1020
BspCNI	CTCAG	6 cut(s)	171, 729, 916, 926, 1096, 1252
BspFNI	CGCG	1 cut(s)	1045
BspHI	TCATGA	1 cut(s)	253
BspLI	GGNNCC	4 cut(s)	403, 1079, 1665, 1855
BspPI	GGATC	2 cut(s)	875, 1555
BspQI	GCTCTTC	1 cut(s)	442
BspT107I	GGYRCC	2 cut(s)	1077, 1853
BspTI	CTTAAG	1 cut(s)	1273
BspTNI	GGTCTC	1 cut(s)	101
BsrFI	RCCGGY	2 cut(s)	1020, 1850
BsrI	ACTGG	5 cut(s)	289, 349, 463, 822, 904
BssAI	RCCGGY	2 cut(s)	1020, 1850
BssECI	CCNNGG	3 cut(s)	538, 1012, 1457
BssMI	GATC	5 cut(s)	161, 180, 394, 880, 1560
BssNI	GRCGYC	3 cut(s)	431, 1078, 1854
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	1 cut(s)	1457
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	3 cut(s)	540, 1014, 1050
Bst4CI	ACNGT	5 cut(s)	374, 498, 611, 1488, 1598
Bst6I	CTCTTC	4 cut(s)	219, 442, 1521, 1786
BstACI	GRCGYC	3 cut(s)	431, 1078, 1854
BstAFI	CTTAAG	1 cut(s)	1273
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstC8I	GCNNGC	4 cut(s)	294, 891, 1022, 1831
BstDEI	CTNAG	8 cut(s)	158, 716, 899, 913, 924, 1104, 1260, 1363
BstDSI	CCRYGG	1 cut(s)	1457
BstF5I	GGATG	3 cut(s)	205, 952, 1067
BstFNI	CGCG	1 cut(s)	1045
BstH2I	RGCGCY	2 cut(s)	1081, 1857
BstHHI	GCGC	5 cut(s)	13, 307, 600, 1080, 1856
BstKTI	GATC	5 cut(s)	164, 183, 397, 883, 1563
BstMAI	GTCTC	2 cut(s)	101, 1512
BstMBI	GATC	5 cut(s)	161, 180, 394, 880, 1560
BstMCI	CGRYCG	2 cut(s)	97, 183
BstMWI	GCNNNNNNNGC	4 cut(s)	302, 646, 1292, 1826
BstNI	CCWGG	3 cut(s)	540, 1014, 1050
BstNSI	RCATGY	1 cut(s)	893
BstPAI	GACNNNNGTC	1 cut(s)	113
BstSCI	CCNGG	3 cut(s)	538, 1012, 1048
BstUI	CGCG	1 cut(s)	1045
BstV1I	GCAGC	8 cut(s)	215, 301, 582, 1176, 1588, 1664, 1711, 1733
BstX2I	RGATCY	2 cut(s)	394, 1560
BstXI	CCANNNNNNTGG	1 cut(s)	539
BstYI	RGATCY	2 cut(s)	394, 1560
BsuI	GTATCC	2 cut(s)	582, 814
BsuRI	GGCC	1 cut(s)	1020
BtgI	CCRYGG	1 cut(s)	1457
BtgZI	GCGATG	1 cut(s)	768
BtrI	CACGTC	1 cut(s)	8
BtsCI	GGATG	3 cut(s)	205, 952, 1067
BtsI	GCAGTG	1 cut(s)	147
BtsIMutI	CAGTG	4 cut(s)	147, 911, 921, 1484
Cac8I	GCNNGC	4 cut(s)	294, 891, 1022, 1831
CaiI	CAGNNNCTG	1 cut(s)	1298
CciI	TCATGA	1 cut(s)	253
CfoI	GCGC	5 cut(s)	13, 307, 600, 1080, 1856
Cfr10I	RCCGGY	2 cut(s)	1020, 1850
Cfr13I	GGNCC	2 cut(s)	802, 1064
CseI	GACGC	3 cut(s)	439, 1034, 1290
Csp6I	GTAC	7 cut(s)	385, 612, 730, 1110, 1155, 1401, 1467
CviAII	CATG	9 cut(s)	254, 413, 809, 890, 991, 1346, 1355, 1458, 1640
CviQI	GTAC	7 cut(s)	385, 612, 730, 1110, 1155, 1401, 1467
DdeI	CTNAG	8 cut(s)	158, 716, 899, 913, 924, 1104, 1260, 1363
DinI	GGCGCC	2 cut(s)	1079, 1855
DpnI	GATC	5 cut(s)	163, 182, 396, 882, 1562
DpnII	GATC	5 cut(s)	161, 180, 394, 880, 1560
DraIII	CACNNNGTG	1 cut(s)	623
EaeI	YGGCCR	1 cut(s)	1018
Eam1104I	CTCTTC	4 cut(s)	219, 442, 1521, 1786
EarI	CTCTTC	4 cut(s)	219, 442, 1521, 1786
Ecl136II	GAGCTC	1 cut(s)	1286
Eco130I	CCWWGG	1 cut(s)	1457
Eco24I	GRGCYC	1 cut(s)	1288
Eco31I	GGTCTC	1 cut(s)	101
Eco47I	GGWCC	2 cut(s)	802, 1064
Eco53kI	GAGCTC	1 cut(s)	1286
Eco57I	CTGAAG	2 cut(s)	218, 1425
Eco88I	CYCGRG	3 cut(s)	329, 1247, 1681
EcoICRI	GAGCTC	1 cut(s)	1286
EcoRII	CCWGG	3 cut(s)	538, 1012, 1048
EcoT14I	CCWWGG	1 cut(s)	1457
EcoT22I	ATGCAT	1 cut(s)	891
EcoT38I	GRGCYC	1 cut(s)	1288
EgeI	GGCGCC	2 cut(s)	1079, 1855
EheI	GGCGCC	2 cut(s)	1079, 1855
ErhI	CCWWGG	1 cut(s)	1457
FaeI	CATG	9 cut(s)	257, 416, 812, 893, 994, 1349, 1358, 1461, 1643
FalI	AAGNNNNNCTT	2 cut(s)	216, 248
FaqI	GGGAC	2 cut(s)	43, 383
FatI	CATG	9 cut(s)	253, 412, 808, 889, 990, 1345, 1354, 1457, 1639
FauI	CCCGC	2 cut(s)	359, 1838
FauNDI	CATATG	2 cut(s)	507, 775
FblI	GTMKAC	1 cut(s)	246
FokI	GGATG	3 cut(s)	212, 959, 1054
FriOI	GRGCYC	1 cut(s)	1288
FspBI	CTAG	5 cut(s)	453, 585, 630, 1002, 1551
FspI	TGCGCA	1 cut(s)	599
GlaI	GCGC	5 cut(s)	12, 306, 599, 1079, 1855
GsuI	CTGGAG	3 cut(s)	366, 1248, 1275
HaeII	RGCGCY	2 cut(s)	1081, 1857
HaeIII	GGCC	1 cut(s)	1020
HapII	CCGG	5 cut(s)	1021, 1573, 1605, 1851, 1885
HgaI	GACGC	3 cut(s)	439, 1034, 1290
HhaI	GCGC	5 cut(s)	13, 307, 600, 1080, 1856
Hin1I	GRCGYC	3 cut(s)	431, 1078, 1854
Hin1II	CATG	9 cut(s)	257, 416, 812, 893, 994, 1349, 1358, 1461, 1643
Hin6I	GCGC	5 cut(s)	11, 305, 598, 1078, 1854
HinP1I	GCGC	5 cut(s)	11, 305, 598, 1078, 1854
HincII	GTYRAC	1 cut(s)	247
HindII	GTYRAC	1 cut(s)	247
HindIII	AAGCTT	2 cut(s)	214, 1415
HpaII	CCGG	5 cut(s)	1021, 1573, 1605, 1851, 1885
HphI	GGTGA	6 cut(s)	318, 584, 1223, 1421, 1840, 1851
Hpy188III	TCNNGA	5 cut(s)	254, 630, 1097, 1220, 1683
HpyAV	CCTTC	4 cut(s)	72, 76, 89, 121
HpyCH4III	ACNGT	5 cut(s)	374, 498, 611, 1488, 1598
HpyCH4IV	ACGT	7 cut(s)	7, 56, 132, 299, 1112, 1170, 1542
HpyF10VI	GCNNNNNNNGC	4 cut(s)	302, 646, 1292, 1826
HpyF3I	CTNAG	8 cut(s)	158, 716, 899, 913, 924, 1104, 1260, 1363
HpySE526I	ACGT	7 cut(s)	7, 56, 132, 299, 1112, 1170, 1542
Hsp92I	GRCGYC	3 cut(s)	431, 1078, 1854
Hsp92II	CATG	9 cut(s)	257, 416, 812, 893, 994, 1349, 1358, 1461, 1643
HspAI	GCGC	5 cut(s)	11, 305, 598, 1078, 1854
KasI	GGCGCC	2 cut(s)	1077, 1853
KroI	GCCGGC	1 cut(s)	1020
KroNI	GCCGGC	1 cut(s)	1022
Kzo9I	GATC	5 cut(s)	161, 180, 394, 880, 1560
LguI	GCTCTTC	1 cut(s)	442
LmnI	GCTCC	5 cut(s)	166, 1283, 1291, 1636, 1732
Lsp1109I	GCAGC	8 cut(s)	215, 301, 582, 1176, 1588, 1664, 1711, 1733
LweI	GCATC	6 cut(s)	190, 745, 781, 1176, 1213, 1261
MaeI	CTAG	5 cut(s)	453, 585, 630, 1002, 1551
MaeII	ACGT	7 cut(s)	7, 56, 132, 299, 1112, 1170, 1542
MaeIII	GTNAC	5 cut(s)	175, 471, 572, 671, 1896
MalI	GATC	5 cut(s)	163, 182, 396, 882, 1562
MboI	GATC	5 cut(s)	161, 180, 394, 880, 1560
MfeI	CAATTG	2 cut(s)	1395, 1731
MflI	RGATCY	2 cut(s)	394, 1560
MhlI	GDGCHC	2 cut(s)	651, 1288
MluCI	AATT	9 cut(s)	142, 357, 377, 759, 1204, 1241, 1374, 1395, 1731
Mly113I	GGCGCC	2 cut(s)	1078, 1854
MlyI	GAGTC	4 cut(s)	479, 569, 1147, 1849
MmeI	TCCRAC	2 cut(s)	114, 1764
Mph1103I	ATGCAT	1 cut(s)	891
MroNI	GCCGGC	1 cut(s)	1020
MseI	TTAA	3 cut(s)	513, 767, 1274
MspA1I	CMGCKG	4 cut(s)	242, 595, 1724, 1780
MspCI	CTTAAG	1 cut(s)	1273
MspI	CCGG	5 cut(s)	1021, 1573, 1605, 1851, 1885
MspR9I	CCNGG	3 cut(s)	540, 1014, 1050
MunI	CAATTG	2 cut(s)	1395, 1731
Mva1269I	GAATGC	1 cut(s)	1588
MvaI	CCWGG	3 cut(s)	540, 1014, 1050
MvnI	CGCG	1 cut(s)	1045
MwoI	GCNNNNNNNGC	4 cut(s)	302, 646, 1292, 1826
NaeI	GCCGGC	1 cut(s)	1022
NarI	GGCGCC	2 cut(s)	1078, 1854
NcoI	CCATGG	1 cut(s)	1457
NdeI	CATATG	2 cut(s)	507, 775
NdeII	GATC	5 cut(s)	161, 180, 394, 880, 1560
NgoMIV	GCCGGC	1 cut(s)	1020
NlaIII	CATG	9 cut(s)	257, 416, 812, 893, 994, 1349, 1358, 1461, 1643
NlaIV	GGNNCC	4 cut(s)	403, 1079, 1665, 1855
NmuCI	GTSAC	4 cut(s)	175, 471, 572, 1896
NsbI	TGCGCA	1 cut(s)	599
NsiI	ATGCAT	1 cut(s)	891
NspI	RCATGY	1 cut(s)	893
PaeI	GCATGC	1 cut(s)	893
PaeR7I	CTCGAG	2 cut(s)	1247, 1681
PagI	TCATGA	1 cut(s)	253
PciSI	GCTCTTC	1 cut(s)	442
PctI	GAATGC	1 cut(s)	1588
PdiI	GCCGGC	1 cut(s)	1022
PfeI	GAWTC	8 cut(s)	34, 416, 686, 707, 785, 998, 1100, 1178
PflMI	CCANNNNNTGG	2 cut(s)	848, 1073
Ple19I	CGATCG	1 cut(s)	183
PleI	GAGTC	4 cut(s)	478, 569, 1146, 1848
PluTI	GGCGCC	2 cut(s)	1081, 1857
PpsI	GAGTC	4 cut(s)	478, 569, 1146, 1848
PshAI	GACNNNNGTC	1 cut(s)	113
PshBI	ATTAAT	1 cut(s)	513
Psp124BI	GAGCTC	1 cut(s)	1288
Psp1406I	AACGTT	1 cut(s)	1542
Psp6I	CCWGG	3 cut(s)	538, 1012, 1048
PspGI	CCWGG	3 cut(s)	538, 1012, 1048
PspN4I	GGNNCC	4 cut(s)	403, 1079, 1665, 1855
PspPI	GGNCC	2 cut(s)	802, 1064
PspXI	VCTCGAGB	1 cut(s)	1247
PstNI	CAGNNNCTG	1 cut(s)	1298
PsuI	RGATCY	2 cut(s)	394, 1560
PvuI	CGATCG	1 cut(s)	183
PvuII	CAGCTG	1 cut(s)	1724
RsaI	GTAC	7 cut(s)	386, 613, 731, 1111, 1156, 1402, 1468
RsaNI	GTAC	7 cut(s)	385, 612, 730, 1110, 1155, 1401, 1467
SacI	GAGCTC	1 cut(s)	1288
SalI	GTCGAC	1 cut(s)	245
SapI	GCTCTTC	1 cut(s)	442
SaqAI	TTAA	3 cut(s)	513, 767, 1274
Sau3AI	GATC	5 cut(s)	161, 180, 394, 880, 1560
Sau96I	GGNCC	2 cut(s)	802, 1064
SchI	GAGTC	4 cut(s)	479, 569, 1147, 1849
ScrFI	CCNGG	3 cut(s)	540, 1014, 1050
SduI	GDGCHC	2 cut(s)	651, 1288
SfaNI	GCATC	6 cut(s)	190, 745, 781, 1176, 1213, 1261
SfoI	GGCGCC	2 cut(s)	1079, 1855
Sfr274I	CTCGAG	2 cut(s)	1247, 1681
SgrAI	CRCCGGYG	1 cut(s)	1850
SinI	GGWCC	2 cut(s)	802, 1064
SlaI	CTCGAG	2 cut(s)	1247, 1681
SmlI	CTYRAG	4 cut(s)	476, 1247, 1273, 1681
SmoI	CTYRAG	4 cut(s)	476, 1247, 1273, 1681
SphI	GCATGC	1 cut(s)	893
Sse9I	AATT	9 cut(s)	142, 357, 377, 759, 1204, 1241, 1374, 1395, 1731
SsiI	CCGC	7 cut(s)	240, 366, 523, 593, 640, 1778, 1831
SspDI	GGCGCC	2 cut(s)	1077, 1853
SspMI	CTAG	5 cut(s)	453, 585, 630, 1002, 1551
SstI	GAGCTC	1 cut(s)	1288
StyD4I	CCNGG	3 cut(s)	538, 1012, 1048
StyI	CCWWGG	1 cut(s)	1457
TaaI	ACNGT	5 cut(s)	374, 498, 611, 1488, 1598
TaiI	ACGT	7 cut(s)	10, 59, 135, 302, 1115, 1173, 1545
TaqI	TCGA	6 cut(s)	32, 37, 246, 1248, 1492, 1682
TaqII	GACCGA	1 cut(s)	111
TasI	AATT	9 cut(s)	142, 357, 377, 759, 1204, 1241, 1374, 1395, 1731
TatI	WGTACW	1 cut(s)	1466
TauI	GCSGC	2 cut(s)	526, 643
TfiI	GAWTC	8 cut(s)	34, 416, 686, 707, 785, 998, 1100, 1178
Tru1I	TTAA	3 cut(s)	513, 767, 1274
Tru9I	TTAA	3 cut(s)	513, 767, 1274
TscAI	CASTG	4 cut(s)	154, 911, 928, 1491
TseFI	GTSAC	4 cut(s)	175, 471, 572, 1896
TseI	GCWGC	8 cut(s)	203, 289, 595, 1189, 1576, 1652, 1721, 1724
Tsp45I	GTSAC	4 cut(s)	175, 471, 572, 1896
TspDTI	ATGAA	6 cut(s)	71, 291, 731, 963, 1106, 1641
TspGWI	ACGGA	2 cut(s)	1486, 1552
TspRI	CASTG	4 cut(s)	154, 911, 928, 1491
Van91I	CCANNNNNTGG	2 cut(s)	848, 1073
Vha464I	CTTAAG	1 cut(s)	1273
VpaK11BI	GGWCC	2 cut(s)	802, 1064
VspI	ATTAAT	1 cut(s)	513
XapI	RAATTY	3 cut(s)	142, 377, 1204
XbaI	TCTAGA	1 cut(s)	629
XceI	RCATGY	1 cut(s)	893
XhoI	CTCGAG	2 cut(s)	1247, 1681
XmiI	GTMKAC	1 cut(s)	246
XspI	CTAG	5 cut(s)	453, 585, 630, 1002, 1551
Zsp2I	ATGCAT	1 cut(s)	891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.