RchiOBHm_Chr3g0459741

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009476
Physical Location & Seq
Reverse (-)
8123534 .. 8126321
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42633

Sequence Viewer

Length: 1617 bp
ATGGTGGCTGACCATTCTGTGGAACCTGCAACATGTGCTCGGTGCCACAGGGCTCTTTCACCCGAAAATGAGTCAATTGGAGATCTTGTAACTGCCAACATGTGTGGGGACTGTAAATTTTTGTACTTTGAAGATCTTGGTACCCCCACACGGGACTCTTATAGGATGAGGCCGTCTAGGAGAAGAAGAGCTAGATACAGTAGTTCCGAGTCGGTTGACGATGTTCTCTCACATTTCTCACATATGATTAATCTGGTGCGGCAAAATCAGTCCCCTGGCTCTGGGCTTGAGCATCTACCTGTAGATGGTGATTCTGCTGCCAGGATATCGCAGCGCACAAGTTCCCGTACTACACCAACTGGGTCTAGAAGATGGAGGCGGGTTCTCTCTGATAGTGAGAGTGATGGTTTCGATAATGCGGATTCTCTCTATGGGGAGAGTGAATCAAATGTCAGTTTTGGGCGGTACAGGGTGTCTCATGATGTGAGTGATGTAGTTTCTTTTAGTGCCTATGGTGGTGATTCTGATGCTTCTGTAGACCTGCATGGTTTCCTGGATACAGAGACATTGATCCAACCAGATGATGGAAGCGCTTTTGATAGTGATACTGACATTGATCCAATGCATGCTGGTCTCAACCCATGGAACTCAGATGACGCTGAAGAAGATGATGACGATGACGATGATGAGGAGGATGAAGAAGATGATGAATGGGAAGAAGTTGAGTCTGTGGAAAACATGGTTGAATCTGCAGAAGCCAATTCTGGTAGAAGAAACCGCCCAGTCAATTGGCACCAACGAAGACATATTACTGAAACTGATGCTCCACTTCATTGGGGAATGAGGGGACGGAATTCCCGTGATATCTTATCTAACTTCGATGATTATGTTACAGATTCTGGTGATTATCTTGATGCAGTAGGCTTTGAAGAATTGCTGGAGCATCTTGCTGAGGCAGACAGCTTAAGACGAGGAGCACCTCCTGCTGCTGTCTCGTTTTTGAATAATCTGCCTCTCATTCTTATTGGTAAGGAACATGAGAAGCATGATGACTTAGCCTGTGCAATTTGCAAGGATGTCTTGACTATTGGCACTGAAGTGAATCAGCTTCCTTGCTTTCACTTGTATCACCCTTCCTGTATTCTGCCATGGTTGAGTACACGGAATTCTTGCCCCCTTTGTCGTTACGAGCTACCAACTGATGACAGAGACTACGAAGAGGGGAAGCGGAATGCTGGTGGTAGAGTGGAGATCCACGTCCGGCGGAATGCTAGAGAGGACAGGTCTGCTGGTGTTTATAATAGAGCTGAAGAAGATGAAGATCATGCTTTTAGTGAAACTAGAGTGGAGCATAGGTTCCAAGTAGGTAGGGAACCTGACTCTAATACCTCCGGAGGAGAAAATAATCGGGGGAGTTGGTTTCTTTTTGCCGCTGCTCCAATTGTTAGTCTTGTGGGCATAGTTCTCGTGCTTTGGTTGGGAGCTCCCCTAATGGAAAGAAGAGGTCTGGCAGGACAGGGCAACTTTGCTGATCAGGCCCGACGCCACGGTCATATTGCTGGTGCAGCACCCCACCACAGGCAGAATAGAAGCAGGAGGTGGTGGTCTCTTTTCTGA

Protein Analysis

538

Amino Acids

60.46

Weight (kDa)

4.82

Isoelectric Point (pI)

57.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 353 - 395 8.6e-11 Ring finger domain
zf-C3HC4_2 PF13923 354 - 394 4.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 354 - 395 4.7e-06 RING-H2 zinc finger domain
zf-C3HC4 PF00097 354 - 394 6.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
AasI GACNNNNNNGTC 1 cut(s) 1548
Acc36I ACCTGC 2 cut(s) 34, 549
Acc65I GGTACC 1 cut(s) 140
AccB1I GGYRCC 3 cut(s) 42, 140, 792
AccB7I CCANNNNNTGG 2 cut(s) 19, 584
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 1391
AciI CCGC 8 cut(s) 259, 379, 419, 463, 778, 1228, 1264, 1431
AclWI GGATC 3 cut(s) 565, 611, 1246
AcsI RAATTY 3 cut(s) 116, 853, 1165
AcuI CTGAAG 3 cut(s) 681, 1116, 1329
AcyI GRCGYC 1 cut(s) 1543
AfaI GTAC 5 cut(s) 125, 142, 349, 467, 1159
AfeI AGCGCT 1 cut(s) 592
AfiI CCNNNNNNNGG 7 cut(s) 19, 150, 151, 281, 305, 584, 1578
AflII CTTAAG 1 cut(s) 964
AflIII ACRYGT 2 cut(s) 32, 99
AgsI TTSAA 4 cut(s) 131, 746, 929, 1003
AjiI CACGTC 1 cut(s) 1258
AjnI CCWGG 3 cut(s) 274, 320, 552
AleI CACNNNNGTG 1 cut(s) 1097
AloI GAACNNNNNNTCC 2 cut(s) 1340, 1372
AluBI AGCT 6 cut(s) 191, 963, 1108, 1192, 1307, 1484
AluI AGCT 6 cut(s) 191, 963, 1108, 1192, 1307, 1484
Alw21I GWGCWC 3 cut(s) 40, 979, 1486
Alw26I GTCTC 6 cut(s) 480, 557, 638, 997, 1203, 1611
AlwI GGATC 3 cut(s) 565, 611, 1246
AlwNI CAGNNNCTG 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 1391
Aor51HI AGCGCT 1 cut(s) 592
AoxI GGCC 2 cut(s) 170, 1536
ApeKI GCWGC 5 cut(s) 317, 331, 986, 1433, 1565
ApoI RAATTY 3 cut(s) 116, 853, 1165
AseI ATTAAT 1 cut(s) 249
Asp718I GGTACC 1 cut(s) 140
AspLEI GCGC 2 cut(s) 336, 593
AspS9I GGNCC 1 cut(s) 1537
AsuHPI GGTGA 5 cut(s) 51, 320, 530, 914, 1121
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BanI GGYRCC 3 cut(s) 42, 140, 792
BanII GRGCYC 2 cut(s) 55, 1486
BarI GAAGNNNNNNTAC 2 cut(s) 793, 825
BauI CACGAG 1 cut(s) 1466
BbsI GAAGAC 1 cut(s) 808
Bbv12I GWGCWC 3 cut(s) 40, 979, 1486
BbvCI CCTCAGC 1 cut(s) 951
BbvI GCAGC 5 cut(s) 304, 343, 973, 1420, 1577
BccI CCATC 4 cut(s) 299, 366, 398, 578
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 1447, 1481
BciT130I CCWGG 3 cut(s) 276, 322, 554
BciVI GTATCC 1 cut(s) 550
BclI TGATCA 1 cut(s) 1531
BcoDI GTCTC 6 cut(s) 480, 557, 638, 997, 1203, 1611
BfaI CTAG 5 cut(s) 177, 192, 366, 1272, 1341
BfmI CTRYAG 3 cut(s) 300, 534, 750
BfoI RGCGCY 1 cut(s) 594
BfrI CTTAAG 1 cut(s) 964
BfuAI ACCTGC 2 cut(s) 34, 549
BfuI GTATCC 1 cut(s) 550
BglII AGATCT 2 cut(s) 82, 133
BisI GCNGC 7 cut(s) 260, 318, 332, 987, 1431, 1434, 1566
BlsI GCNGC 7 cut(s) 261, 319, 333, 988, 1432, 1435, 1567
Bme1390I CCNGG 3 cut(s) 276, 322, 554
BmgBI CACGTC 1 cut(s) 1258
BmgT120I GGNCC 1 cut(s) 1537
BmiI GGNNCC 6 cut(s) 24, 44, 142, 794, 1358, 1374
BmrFI CCNGG 3 cut(s) 276, 322, 554
BmrI ACTGGG 2 cut(s) 369, 776
BmsI GCATC 5 cut(s) 301, 517, 811, 904, 952
BmuI ACTGGG 2 cut(s) 369, 776
BpiI GAAGAC 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 959
Bpu10I CCTNAGC 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 1320
BsaHI GRCGYC 1 cut(s) 1543
BsaI GGTCTC 2 cut(s) 638, 1611
BsaJI CCNNGG 4 cut(s) 274, 641, 1148, 1546
BsaWI WCCGGW 1 cut(s) 1391
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 808, 838, 1340, 1370
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 150, 151, 281, 305, 584, 1578
Bse1I ACTGG 2 cut(s) 364, 782
Bse8I GATNNNNATC 1 cut(s) 1320
BseAI TCCGGA 1 cut(s) 1391
BseBI CCWGG 3 cut(s) 276, 322, 554
BseDI CCNNGG 4 cut(s) 274, 641, 1148, 1546
BseGI GGATG 3 cut(s) 171, 700, 1081
BseJI GATNNNNATC 1 cut(s) 1320
BseLI CCNNNNNNNGG 7 cut(s) 19, 150, 151, 281, 305, 584, 1578
BseMII CTCAG 2 cut(s) 663, 942
BseNI ACTGG 2 cut(s) 364, 782
BseRI GAGGAG 3 cut(s) 704, 987, 1410
BseXI GCAGC 5 cut(s) 304, 343, 973, 1420, 1577
BsgI GTGCAG 1 cut(s) 1584
BshFI GGCC 2 cut(s) 172, 1538
BshNI GGYRCC 3 cut(s) 42, 140, 792
BsiHKAI GWGCWC 3 cut(s) 40, 979, 1486
BsiSI CCGG 2 cut(s) 1261, 1392
BslFI GGGAC 4 cut(s) 122, 167, 256, 861
BslI CCNNNNNNNGG 7 cut(s) 19, 150, 151, 281, 305, 584, 1578
BsmAI GTCTC 6 cut(s) 480, 557, 638, 997, 1203, 1611
BsmFI GGGAC 4 cut(s) 122, 167, 256, 861
BsmI GAATGC 2 cut(s) 1237, 1273
BsnI GGCC 2 cut(s) 172, 1538
Bso31I GGTCTC 2 cut(s) 638, 1611
Bsp1286I GDGCHC 4 cut(s) 40, 55, 979, 1486
Bsp13I TCCGGA 1 cut(s) 1391
Bsp143I GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
Bsp19I CCATGG 2 cut(s) 641, 1148
BspACI CCGC 8 cut(s) 259, 379, 419, 463, 778, 1228, 1264, 1431
BspANI GGCC 2 cut(s) 172, 1538
BspCNI CTCAG 2 cut(s) 662, 943
BspEI TCCGGA 1 cut(s) 1391
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 6 cut(s) 24, 44, 142, 794, 1358, 1374
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 2 cut(s) 34, 549
BspPI GGATC 3 cut(s) 565, 611, 1246
BspQI GCTCTTC 1 cut(s) 181
BspT107I GGYRCC 3 cut(s) 42, 140, 792
BspTI CTTAAG 1 cut(s) 964
BspTNI GGTCTC 2 cut(s) 638, 1611
BsrI ACTGG 2 cut(s) 364, 782
BssECI CCNNGG 4 cut(s) 274, 641, 1148, 1546
BssMI GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
BssNI GRCGYC 1 cut(s) 1543
BssSI CACGAG 1 cut(s) 1466
BssT1I CCWWGG 2 cut(s) 641, 1148
Bst2BI CACGAG 1 cut(s) 1466
Bst2UI CCWGG 3 cut(s) 276, 322, 554
Bst4CI ACNGT 3 cut(s) 113, 200, 1550
Bst6I CTCTTC 3 cut(s) 181, 1212, 1495
BstACI GRCGYC 1 cut(s) 1543
BstAFI CTTAAG 1 cut(s) 964
BstAPI GCANNNNNTGC 2 cut(s) 35, 983
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 3 cut(s) 649, 951, 1054
BstDSI CCRYGG 3 cut(s) 641, 1148, 1546
BstF5I GGATG 3 cut(s) 171, 700, 1081
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 2 cut(s) 336, 593
BstKTI GATC 7 cut(s) 85, 136, 573, 619, 1254, 1324, 1534
BstMAI GTCTC 6 cut(s) 480, 557, 638, 997, 1203, 1611
BstMBI GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
BstMWI GCNNNNNNNGC 4 cut(s) 35, 983, 1535, 1565
BstNI CCWGG 3 cut(s) 276, 322, 554
BstNSI RCATGY 3 cut(s) 36, 103, 629
BstSCI CCNGG 3 cut(s) 274, 320, 552
BstSFI CTRYAG 3 cut(s) 300, 534, 750
BstV1I GCAGC 5 cut(s) 304, 343, 973, 1420, 1577
BstV2I GAAGAC 1 cut(s) 808
BstX2I RGATCY 3 cut(s) 82, 133, 1251
BstXI CCANNNNNNTGG 2 cut(s) 789, 834
BstYI RGATCY 3 cut(s) 82, 133, 1251
BsuI GTATCC 1 cut(s) 550
BsuRI GGCC 2 cut(s) 172, 1538
BtgI CCRYGG 3 cut(s) 641, 1148, 1546
BtrI CACGTC 1 cut(s) 1258
BtsCI GGATG 3 cut(s) 171, 700, 1081
BtsIMutI CAGTG 1 cut(s) 1092
BveI ACCTGC 2 cut(s) 34, 549
Cac8I GCNNGC 1 cut(s) 627
CaiI CAGNNNCTG 1 cut(s) 899
CciI TCATGA 1 cut(s) 478
CfoI GCGC 2 cut(s) 336, 593
Cfr13I GGNCC 1 cut(s) 1537
CseI GACGC 2 cut(s) 665, 1551
Csp6I GTAC 5 cut(s) 124, 141, 348, 466, 1158
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviQI GTAC 5 cut(s) 124, 141, 348, 466, 1158
DdeI CTNAG 3 cut(s) 649, 951, 1054
DpnI GATC 7 cut(s) 84, 135, 572, 618, 1253, 1323, 1533
DpnII GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
DrdI GACNNNNNNGTC 1 cut(s) 1548
DseDI GACNNNNNNGTC 1 cut(s) 1548
Eam1104I CTCTTC 3 cut(s) 181, 1212, 1495
EarI CTCTTC 3 cut(s) 181, 1212, 1495
EciI GGCGGA 1 cut(s) 1279
Ecl136II GAGCTC 1 cut(s) 1484
Eco130I CCWWGG 2 cut(s) 641, 1148
Eco24I GRGCYC 2 cut(s) 55, 1486
Eco31I GGTCTC 2 cut(s) 638, 1611
Eco32I GATATC 2 cut(s) 327, 865
Eco47III AGCGCT 1 cut(s) 592
Eco53kI GAGCTC 1 cut(s) 1484
Eco57I CTGAAG 3 cut(s) 681, 1116, 1329
EcoICRI GAGCTC 1 cut(s) 1484
EcoRI GAATTC 2 cut(s) 853, 1165
EcoRII CCWGG 3 cut(s) 274, 320, 552
EcoRV GATATC 2 cut(s) 327, 865
EcoT14I CCWWGG 2 cut(s) 641, 1148
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 2 cut(s) 55, 1486
ErhI CCWWGG 2 cut(s) 641, 1148
FalI AAGNNNNNCTT 2 cut(s) 1064, 1096
FaqI GGGAC 4 cut(s) 122, 167, 256, 861
FauI CCCGC 1 cut(s) 372
FauNDI CATATG 1 cut(s) 243
FbaI TGATCA 1 cut(s) 1531
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 7 cut(s) 260, 318, 332, 987, 1431, 1434, 1566
FokI GGATG 3 cut(s) 178, 707, 1088
FriOI GRGCYC 2 cut(s) 55, 1486
Fsp4HI GCNGC 7 cut(s) 260, 318, 332, 987, 1431, 1434, 1566
FspBI CTAG 5 cut(s) 177, 192, 366, 1272, 1341
GlaI GCGC 2 cut(s) 335, 592
GluI GCNGC 7 cut(s) 260, 318, 332, 987, 1431, 1434, 1566
GsuI CTGGAG 1 cut(s) 959
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 2 cut(s) 172, 1538
HapII CCGG 2 cut(s) 1261, 1392
HgaI GACGC 2 cut(s) 665, 1551
HhaI GCGC 2 cut(s) 336, 593
Hin1I GRCGYC 1 cut(s) 1543
Hin6I GCGC 2 cut(s) 334, 591
HinP1I GCGC 2 cut(s) 334, 591
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HpaII CCGG 2 cut(s) 1261, 1392
HphI GGTGA 5 cut(s) 51, 320, 530, 914, 1121
Hpy166II GTNNAC 3 cut(s) 217, 538, 1160
Hpy188I TCNGA 5 cut(s) 208, 391, 526, 652, 1616
Hpy188III TCNNGA 5 cut(s) 366, 479, 911, 1081, 1392
Hpy8I GTNNAC 3 cut(s) 217, 538, 1160
Hpy99I CGWCG 1 cut(s) 1545
HpyAV CCTTC 1 cut(s) 1143
HpyCH4III ACNGT 3 cut(s) 113, 200, 1550
HpyCH4IV ACGT 1 cut(s) 1257
HpyCH4V TGCA 8 cut(s) 29, 544, 625, 752, 917, 1064, 1071, 1565
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 983, 1535, 1565
HpyF3I CTNAG 3 cut(s) 649, 951, 1054
HpySE526I ACGT 1 cut(s) 1257
Hsp92I GRCGYC 1 cut(s) 1543
HspAI GCGC 2 cut(s) 334, 591
Kpn2I TCCGGA 1 cut(s) 1391
KpnI GGTACC 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 1531
Kzo9I GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
LguI GCTCTTC 1 cut(s) 181
LmnI GCTCC 7 cut(s) 829, 940, 974, 1348, 1441, 1481, 1489
Lsp1109I GCAGC 5 cut(s) 304, 343, 973, 1420, 1577
LweI GCATC 5 cut(s) 301, 517, 811, 904, 952
MaeI CTAG 5 cut(s) 177, 192, 366, 1272, 1341
MaeII ACGT 1 cut(s) 1257
MaeIII GTNAC 3 cut(s) 88, 889, 1184
MalI GATC 7 cut(s) 84, 135, 572, 618, 1253, 1323, 1533
MboI GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
MfeI CAATTG 3 cut(s) 75, 787, 1440
MflI RGATCY 3 cut(s) 82, 133, 1251
MhlI GDGCHC 4 cut(s) 40, 55, 979, 1486
MluCI AATT 9 cut(s) 75, 116, 760, 787, 853, 932, 1065, 1165, 1440
MlyI GAGTC 5 cut(s) 80, 149, 218, 734, 1373
MmeI TCCRAC 1 cut(s) 598
Mph1103I ATGCAT 1 cut(s) 627
MroI TCCGGA 1 cut(s) 1391
MseI TTAA 2 cut(s) 249, 965
MslI CAYNNNNRTG 1 cut(s) 1097
MspA1I CMGCKG 1 cut(s) 1433
MspCI CTTAAG 1 cut(s) 964
MspI CCGG 2 cut(s) 1261, 1392
MspR9I CCNGG 3 cut(s) 276, 322, 554
MunI CAATTG 3 cut(s) 75, 787, 1440
Mva1269I GAATGC 2 cut(s) 1237, 1273
MvaI CCWGG 3 cut(s) 276, 322, 554
MwoI GCNNNNNNNGC 4 cut(s) 35, 983, 1535, 1565
NcoI CCATGG 2 cut(s) 641, 1148
NdeI CATATG 1 cut(s) 243
NdeII GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
NlaIV GGNNCC 6 cut(s) 24, 44, 142, 794, 1358, 1374
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 3 cut(s) 36, 103, 629
OliI CACNNNNGTG 1 cut(s) 1097
PaeI GCATGC 1 cut(s) 629
PagI TCATGA 1 cut(s) 478
PciI ACATGT 2 cut(s) 32, 99
PciSI GCTCTTC 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 856
PctI GAATGC 2 cut(s) 1237, 1273
PfeI GAWTC 7 cut(s) 311, 422, 443, 521, 746, 896, 1102
PflMI CCANNNNNTGG 2 cut(s) 19, 584
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 7 cut(s) 261, 319, 333, 988, 1432, 1435, 1567
PleI GAGTC 5 cut(s) 79, 149, 217, 733, 1373
PpsI GAGTC 5 cut(s) 79, 149, 217, 733, 1373
PscI ACATGT 2 cut(s) 32, 99
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 1 cut(s) 1299
Psp124BI GAGCTC 1 cut(s) 1486
Psp6I CCWGG 3 cut(s) 274, 320, 552
PspGI CCWGG 3 cut(s) 274, 320, 552
PspN4I GGNNCC 6 cut(s) 24, 44, 142, 794, 1358, 1374
PspPI GGNCC 1 cut(s) 1537
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 3 cut(s) 82, 133, 1251
RsaI GTAC 5 cut(s) 125, 142, 349, 467, 1159
RsaNI GTAC 5 cut(s) 124, 141, 348, 466, 1158
RseI CAYNNNNRTG 1 cut(s) 1097
SacI GAGCTC 1 cut(s) 1486
SapI GCTCTTC 1 cut(s) 181
SaqAI TTAA 2 cut(s) 249, 965
SatI GCNGC 7 cut(s) 260, 318, 332, 987, 1431, 1434, 1566
Sau3AI GATC 7 cut(s) 82, 133, 570, 616, 1251, 1321, 1531
Sau96I GGNCC 1 cut(s) 1537
SchI GAGTC 5 cut(s) 80, 149, 218, 734, 1373
ScrFI CCNGG 3 cut(s) 276, 322, 554
SduI GDGCHC 4 cut(s) 40, 55, 979, 1486
SfaNI GCATC 5 cut(s) 301, 517, 811, 904, 952
SfcI CTRYAG 3 cut(s) 300, 534, 750
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 2 cut(s) 287, 964
SmoI CTYRAG 2 cut(s) 287, 964
SphI GCATGC 1 cut(s) 629
Sse9I AATT 9 cut(s) 75, 116, 760, 787, 853, 932, 1065, 1165, 1440
SsiI CCGC 8 cut(s) 259, 379, 419, 463, 778, 1228, 1264, 1431
SspMI CTAG 5 cut(s) 177, 192, 366, 1272, 1341
SstI GAGCTC 1 cut(s) 1486
StyD4I CCNGG 3 cut(s) 274, 320, 552
StyI CCWWGG 2 cut(s) 641, 1148
TaaI ACNGT 3 cut(s) 113, 200, 1550
TaiI ACGT 1 cut(s) 1260
TaqI TCGA 2 cut(s) 411, 879
TasI AATT 9 cut(s) 75, 116, 760, 787, 853, 932, 1065, 1165, 1440
TatI WGTACW 2 cut(s) 123, 1157
TauI GCSGC 2 cut(s) 262, 1433
TfiI GAWTC 7 cut(s) 311, 422, 443, 521, 746, 896, 1102
Tru1I TTAA 2 cut(s) 249, 965
Tru9I TTAA 2 cut(s) 249, 965
TscAI CASTG 1 cut(s) 1099
TseI GCWGC 5 cut(s) 317, 331, 986, 1433, 1565
TspDTI ATGAA 4 cut(s) 711, 723, 821, 1332
TspGWI ACGGA 2 cut(s) 865, 1177
TspRI CASTG 1 cut(s) 1099
Van91I CCANNNNNTGG 2 cut(s) 19, 584
Vha464I CTTAAG 1 cut(s) 964
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 3 cut(s) 116, 853, 1165
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 3 cut(s) 36, 103, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 5 cut(s) 177, 192, 366, 1272, 1341
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.