pycom12g18010

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009476
Physical Location & Seq
Forward (+)
20308353 .. 20309900
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18010.1

Sequence Viewer

Length: 1548 bp
ATGTGTGGGGACTGTAAATTTCTGTACCTTGAAGATCCTGGTACCCCCTCACATGATTCTTATCAGAGGATGCCGCCTACGAGAAGAAGAGCTAGGACCAGCAGTTCTGAGTCACTTGAGAATACTTTTTTGCAACAATTCTCGCATATGATTAATCCGGTGCGGCAAAACCAATCCCCTGGCGCTGGGCTTGAGGAACTACCTGTGGATGGTGATTCTGCTGCTAGGATACCGCAGCGCACAAGTAACCGTACTACACCAAGTGGGTCTAGAAGATGGCGGCGGGTACTCTCGGATACTGAAAGTGAGGGTTACGAAAATGTGGATTCTCTCTATGAAGAAACTGAATCAAATCTCAGTTTTGGTCAGTACAGGGCATTTCATGGTGAGAATGATGCAAATTCTTTTAGTGCCTATGGAGGGGACTCTGATGCTTCTGTGGACCTACTTGGTTTCCTGGATATTGAAACGGTTATTCAACCAGATGAGGGAAGTGCTTATGATAGCGATACTGACATTGATCCAATGCATGCCGGTCTTAGCCAGTGGAACTCAGATGACACTGAGGACGATGATGATGAGGGGGATGAAGACGATCATGAATGGGAAGAAGCTGGTGTTGAAGTAAACATAGTTGAATCTAGGGAAACCCCAGGTGGTCGGCTTCGTAATATTTTAGTTTCGAGTCCCGACAGAAGTAATGGCCCATCCAATTGGCTTCAACAAATATATTCTCCTGAATCTGAGGGTATGATTCGTTATAGAATGAGGGGAACCAATCAAGTATATGACCTCTTTTCTAACGTGGAAGAATCAGAGTTGCTGCCTTATGTTGGAAATTCTGGTGATTATCTTGATGCTAGAGGCTTTGAAGAATTGCTCGAGCATCTTGCTGAGGTTGATAGCTTAAGACGAGGAGCACCTCCAGCTTCCGTGTCCCTTGTGAATAATCTGCCTCGTGTTGTCATTAGTAAGGAACATGACAAGCATGATGACTTAGCCTGTGCAATTTGCAAGGATGGATTGCCAATTGGTACTGAAGTGAATCAGCTTCCCTGCTCTCACCTGTATCACTCTTCCTGTATTTTGCCATGGTTGAGTACACGGAATACTTGTCCACTCTGTCGATATGAGCTACCAACAGATGACAGAGACTACGAAGAGGGGAAACAGAACGTTAGTGCTAGAGTGGAGATCCACAACGTCCGGCAGCAGAATGCAAGTGAGGACAGTTCCTCCGGTGCTTTCAACAGAGCTGATGAAGATGAGGATGAAGAGCATGAGCAGAGTGCAGAAAATAGAGGACCAGCCACTGATGGCTCGAGAGGAGAAAATGGGCGGGGGAGATGGTTTTTTCTCGCAGCTGCTCCAATTGTCAGTCTCGTGGGGATTGTTCTTGTGCTGTGGTTAGGAAGTCCCCTGTTGGAAAGACGAAGACTGGCAGGAAACCAGAACTTTGCCAATCAGGCCCGCCGCCAAAGTCATGTCACCGGCGCCTCACCCCACCACAGGGAGAACAGAAGCCGACCATGGTGGTCACTTTTCTGA

Protein Analysis

516

Amino Acids

57.73

Weight (kDa)

4.62

Isoelectric Point (pI)

60.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 334 - 376 2.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 335 - 375 7.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 335 - 375 4.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 335 - 362 1.6e-06 RING-like zinc finger
zf-rbx1 PF12678 347 - 376 4.1e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 41, 1493
AciI CCGC 8 cut(s) 74, 163, 233, 280, 283, 1339, 1471, 1474
AclI AACGTT 1 cut(s) 1176
AclWI GGATC 3 cut(s) 29, 515, 1189
AcsI RAATTY 3 cut(s) 17, 400, 838
AcuI CTGAAG 1 cut(s) 1059
AcyI GRCGYC 1 cut(s) 1494
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 7 cut(s) 26, 43, 253, 288, 371, 1036, 1102
AfiI CCNNNNNNNGG 7 cut(s) 185, 209, 420, 488, 833, 1509, 1510
AflII CTTAAG 1 cut(s) 907
AgsI TTSAA 8 cut(s) 32, 467, 479, 623, 638, 722, 872, 1249
AjnI CCWGG 4 cut(s) 37, 178, 456, 652
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
AluBI AGCT 8 cut(s) 92, 614, 906, 929, 1051, 1135, 1256, 1364
AluI AGCT 8 cut(s) 92, 614, 906, 929, 1051, 1135, 1256, 1364
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 2 cut(s) 1146, 1385
AlwI GGATC 3 cut(s) 29, 515, 1189
AlwNI CAGNNNCTG 1 cut(s) 1313
Ama87I CYCGRG 2 cut(s) 881, 1321
AoxI GGCC 2 cut(s) 703, 1467
ApeKI GCWGC 6 cut(s) 221, 235, 823, 1210, 1361, 1364
ApoI RAATTY 3 cut(s) 17, 400, 838
AseI ATTAAT 1 cut(s) 153
Asp718I GGTACC 1 cut(s) 41
AspLEI GCGC 3 cut(s) 185, 240, 1496
AspS9I GGNCC 5 cut(s) 96, 442, 704, 1304, 1468
AsuHPI GGTGA 6 cut(s) 224, 398, 857, 1055, 1480, 1491
AvaI CYCGRG 2 cut(s) 881, 1321
AvaII GGWCC 3 cut(s) 96, 442, 1304
BaeI ACNNNNGTAYC 2 cut(s) 1026, 1059
BanI GGYRCC 2 cut(s) 41, 1493
BauI CACGAG 2 cut(s) 957, 1382
BbsI GAAGAC 2 cut(s) 597, 1441
Bbv12I GWGCWC 1 cut(s) 922
BbvCI CCTCAGC 1 cut(s) 894
BbvI GCAGC 6 cut(s) 208, 247, 810, 1222, 1351, 1373
BccI CCATC 6 cut(s) 203, 270, 715, 1013, 1310, 1341
BcgI CGANNNNNNTGC 2 cut(s) 872, 906
BciT130I CCWGG 4 cut(s) 39, 180, 458, 654
BciVI GTATCC 2 cut(s) 222, 289
BcoDI GTCTC 2 cut(s) 1146, 1385
BfaI CTAG 6 cut(s) 93, 225, 270, 642, 861, 1185
BfoI RGCGCY 2 cut(s) 186, 1497
BfrI CTTAAG 1 cut(s) 907
BfuI GTATCC 2 cut(s) 222, 289
BglI GCCNNNNNGGC 1 cut(s) 1466
Bme1390I CCNGG 4 cut(s) 39, 180, 458, 654
Bme18I GGWCC 3 cut(s) 96, 442, 1304
BmeT110I CYCGRG 2 cut(s) 881, 1321
BmgT120I GGNCC 5 cut(s) 96, 442, 704, 1304, 1468
BmiI GGNNCC 3 cut(s) 43, 775, 1495
BmrFI CCNGG 4 cut(s) 39, 180, 458, 654
BmsI GCATC 5 cut(s) 60, 385, 421, 847, 895
BpiI GAAGAC 2 cut(s) 597, 1441
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 894
BpuEI CTTGAG 2 cut(s) 137, 212
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 1494
BsaJI CCNNGG 4 cut(s) 178, 652, 1091, 1529
BsaWI WCCGGW 2 cut(s) 157, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 1220, 1250
Bsc4I CCNNNNNNNGG 7 cut(s) 185, 209, 420, 488, 833, 1509, 1510
Bse118I RCCGGY 2 cut(s) 533, 1490
Bse1I ACTGG 2 cut(s) 544, 1443
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 4 cut(s) 39, 180, 458, 654
BseDI CCNNGG 4 cut(s) 178, 652, 1091, 1529
BseGI GGATG 6 cut(s) 75, 214, 592, 707, 1024, 1276
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 7 cut(s) 185, 209, 420, 488, 833, 1509, 1510
BseMII CTCAG 6 cut(s) 99, 370, 555, 567, 735, 885
BseNI ACTGG 2 cut(s) 544, 1443
BseRI GAGGAG 2 cut(s) 930, 1341
BseXI GCAGC 6 cut(s) 208, 247, 810, 1222, 1351, 1373
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 1311
BshFI GGCC 2 cut(s) 705, 1469
BshNI GGYRCC 2 cut(s) 41, 1493
BsiHKAI GWGCWC 1 cut(s) 922
BsiHKCI CYCGRG 2 cut(s) 881, 1321
BsiSI CCGG 5 cut(s) 158, 534, 1207, 1239, 1491
BslFI GGGAC 5 cut(s) 23, 437, 672, 922, 1401
BslI CCNNNNNNNGG 7 cut(s) 185, 209, 420, 488, 833, 1509, 1510
BsmAI GTCTC 2 cut(s) 1146, 1385
BsmFI GGGAC 5 cut(s) 23, 437, 672, 922, 1401
BsmI GAATGC 1 cut(s) 1222
BsnI GGCC 2 cut(s) 705, 1469
BsoBI CYCGRG 2 cut(s) 881, 1321
Bsp1286I GDGCHC 1 cut(s) 922
Bsp143I GATC 4 cut(s) 34, 520, 595, 1194
Bsp19I CCATGG 2 cut(s) 1091, 1529
BspACI CCGC 8 cut(s) 74, 163, 233, 280, 283, 1339, 1471, 1474
BspANI GGCC 2 cut(s) 705, 1469
BspCNI CTCAG 6 cut(s) 100, 369, 556, 566, 736, 886
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 43, 775, 1495
BspPI GGATC 3 cut(s) 29, 515, 1189
BspQI GCTCTTC 2 cut(s) 82, 1269
BspT107I GGYRCC 2 cut(s) 41, 1493
BspTI CTTAAG 1 cut(s) 907
BsrFI RCCGGY 2 cut(s) 533, 1490
BsrI ACTGG 2 cut(s) 544, 1443
BssAI RCCGGY 2 cut(s) 533, 1490
BssECI CCNNGG 4 cut(s) 178, 652, 1091, 1529
BssMI GATC 4 cut(s) 34, 520, 595, 1194
BssNI GRCGYC 1 cut(s) 1494
BssSI CACGAG 2 cut(s) 957, 1382
BssT1I CCWWGG 2 cut(s) 1091, 1529
Bst2BI CACGAG 2 cut(s) 957, 1382
Bst2UI CCWGG 4 cut(s) 39, 180, 458, 654
Bst4CI ACNGT 4 cut(s) 14, 251, 472, 1232
Bst6I CTCTTC 4 cut(s) 82, 1081, 1155, 1269
BstACI GRCGYC 1 cut(s) 1494
BstAFI CTTAAG 1 cut(s) 907
BstC8I GCNNGC 2 cut(s) 531, 1471
BstDEI CTNAG 8 cut(s) 108, 356, 539, 553, 564, 744, 894, 997
BstDSI CCRYGG 2 cut(s) 1091, 1529
BstF5I GGATG 6 cut(s) 75, 214, 592, 707, 1024, 1276
BstH2I RGCGCY 2 cut(s) 186, 1497
BstHHI GCGC 3 cut(s) 185, 240, 1496
BstKTI GATC 4 cut(s) 37, 523, 598, 1197
BstMAI GTCTC 2 cut(s) 1146, 1385
BstMBI GATC 4 cut(s) 34, 520, 595, 1194
BstMWI GCNNNNNNNGC 2 cut(s) 926, 1466
BstNI CCWGG 4 cut(s) 39, 180, 458, 654
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 4 cut(s) 37, 178, 456, 652
BstV1I GCAGC 6 cut(s) 208, 247, 810, 1222, 1351, 1373
BstV2I GAAGAC 2 cut(s) 597, 1441
BstX2I RGATCY 2 cut(s) 34, 1194
BstXI CCANNNNNNTGG 2 cut(s) 179, 714
BstYI RGATCY 2 cut(s) 34, 1194
BsuI GTATCC 2 cut(s) 222, 289
BsuRI GGCC 2 cut(s) 705, 1469
BtgI CCRYGG 2 cut(s) 1091, 1529
BtsCI GGATG 6 cut(s) 75, 214, 592, 707, 1024, 1276
BtsIMutI CAGTG 3 cut(s) 551, 561, 1311
Cac8I GCNNGC 2 cut(s) 531, 1471
CaiI CAGNNNCTG 1 cut(s) 1313
CciI TCATGA 1 cut(s) 598
CfoI GCGC 3 cut(s) 185, 240, 1496
Cfr10I RCCGGY 2 cut(s) 533, 1490
Cfr13I GGNCC 5 cut(s) 96, 442, 704, 1304, 1468
Csp6I GTAC 7 cut(s) 25, 42, 252, 287, 370, 1035, 1101
CviQI GTAC 7 cut(s) 25, 42, 252, 287, 370, 1035, 1101
DdeI CTNAG 8 cut(s) 108, 356, 539, 553, 564, 744, 894, 997
DinI GGCGCC 1 cut(s) 1495
DpnI GATC 4 cut(s) 36, 522, 597, 1196
DpnII GATC 4 cut(s) 34, 520, 595, 1194
DraIII CACNNNGTG 1 cut(s) 263
Eam1104I CTCTTC 4 cut(s) 82, 1081, 1155, 1269
EarI CTCTTC 4 cut(s) 82, 1081, 1155, 1269
Eco130I CCWWGG 2 cut(s) 1091, 1529
Eco47I GGWCC 3 cut(s) 96, 442, 1304
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 2 cut(s) 881, 1321
EcoRII CCWGG 4 cut(s) 37, 178, 456, 652
EcoT14I CCWWGG 2 cut(s) 1091, 1529
EcoT22I ATGCAT 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 1495
EheI GGCGCC 1 cut(s) 1495
ErhI CCWWGG 2 cut(s) 1091, 1529
FaqI GGGAC 5 cut(s) 23, 437, 672, 922, 1401
FauI CCCGC 3 cut(s) 276, 1332, 1478
FauNDI CATATG 1 cut(s) 147
FokI GGATG 6 cut(s) 82, 221, 599, 694, 1031, 1283
FspBI CTAG 6 cut(s) 93, 225, 270, 642, 861, 1185
GlaI GCGC 3 cut(s) 184, 239, 1495
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 909
HaeII RGCGCY 2 cut(s) 186, 1497
HaeIII GGCC 2 cut(s) 705, 1469
HapII CCGG 5 cut(s) 158, 534, 1207, 1239, 1491
HhaI GCGC 3 cut(s) 185, 240, 1496
Hin1I GRCGYC 1 cut(s) 1494
Hin6I GCGC 3 cut(s) 183, 238, 1494
HinP1I GCGC 3 cut(s) 183, 238, 1494
HpaII CCGG 5 cut(s) 158, 534, 1207, 1239, 1491
HphI GGTGA 6 cut(s) 224, 398, 857, 1055, 1480, 1491
Hpy166II GTNNAC 4 cut(s) 442, 628, 1103, 1118
Hpy188I TCNGA 8 cut(s) 66, 109, 295, 430, 556, 745, 817, 1547
Hpy188III TCNNGA 6 cut(s) 270, 599, 689, 737, 854, 1323
Hpy8I GTNNAC 4 cut(s) 442, 628, 1103, 1118
HpyCH4III ACNGT 4 cut(s) 14, 251, 472, 1232
HpyCH4IV ACGT 3 cut(s) 804, 1176, 1203
HpyCH4V TGCA 7 cut(s) 133, 398, 529, 1007, 1014, 1220, 1292
HpyF10VI GCNNNNNNNGC 2 cut(s) 926, 1466
HpyF3I CTNAG 8 cut(s) 108, 356, 539, 553, 564, 744, 894, 997
HpySE526I ACGT 3 cut(s) 804, 1176, 1203
Hsp92I GRCGYC 1 cut(s) 1494
HspAI GCGC 3 cut(s) 183, 238, 1494
KasI GGCGCC 1 cut(s) 1493
KpnI GGTACC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 34, 520, 595, 1194
LguI GCTCTTC 2 cut(s) 82, 1269
LmnI GCTCC 2 cut(s) 917, 1372
Lsp1109I GCAGC 6 cut(s) 208, 247, 810, 1222, 1351, 1373
LweI GCATC 5 cut(s) 60, 385, 421, 847, 895
MaeI CTAG 6 cut(s) 93, 225, 270, 642, 861, 1185
MaeII ACGT 3 cut(s) 804, 1176, 1203
MaeIII GTNAC 5 cut(s) 111, 245, 311, 1486, 1536
MalI GATC 4 cut(s) 36, 522, 597, 1196
MboI GATC 4 cut(s) 34, 520, 595, 1194
MfeI CAATTG 3 cut(s) 712, 1029, 1371
MflI RGATCY 2 cut(s) 34, 1194
MhlI GDGCHC 1 cut(s) 922
MluCI AATT 9 cut(s) 17, 137, 400, 712, 838, 875, 1008, 1029, 1371
Mly113I GGCGCC 1 cut(s) 1494
MlyI GAGTC 3 cut(s) 119, 419, 694
MmeI TCCRAC 2 cut(s) 814, 1404
Mph1103I ATGCAT 1 cut(s) 531
MseI TTAA 2 cut(s) 153, 908
MspA1I CMGCKG 1 cut(s) 1364
MspCI CTTAAG 1 cut(s) 907
MspI CCGG 5 cut(s) 158, 534, 1207, 1239, 1491
MspR9I CCNGG 4 cut(s) 39, 180, 458, 654
MunI CAATTG 3 cut(s) 712, 1029, 1371
Mva1269I GAATGC 1 cut(s) 1222
MvaI CCWGG 4 cut(s) 39, 180, 458, 654
MwoI GCNNNNNNNGC 2 cut(s) 926, 1466
NarI GGCGCC 1 cut(s) 1494
NcoI CCATGG 2 cut(s) 1091, 1529
NdeI CATATG 1 cut(s) 147
NdeII GATC 4 cut(s) 34, 520, 595, 1194
NlaIV GGNNCC 3 cut(s) 43, 775, 1495
NmuCI GTSAC 3 cut(s) 111, 1486, 1536
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PaeR7I CTCGAG 2 cut(s) 881, 1321
PagI TCATGA 1 cut(s) 598
PciSI GCTCTTC 2 cut(s) 82, 1269
PctI GAATGC 1 cut(s) 1222
PfeI GAWTC 9 cut(s) 56, 215, 326, 347, 638, 740, 754, 812, 1045
PfoI TCCNGGA 1 cut(s) 456
PleI GAGTC 3 cut(s) 118, 419, 693
PluTI GGCGCC 1 cut(s) 1497
PpsI GAGTC 3 cut(s) 118, 419, 693
PshBI ATTAAT 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 1176
Psp6I CCWGG 4 cut(s) 37, 178, 456, 652
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 4 cut(s) 37, 178, 456, 652
PspN4I GGNNCC 3 cut(s) 43, 775, 1495
PspPI GGNCC 5 cut(s) 96, 442, 704, 1304, 1468
PspXI VCTCGAGB 1 cut(s) 881
PstNI CAGNNNCTG 1 cut(s) 1313
PsuI RGATCY 2 cut(s) 34, 1194
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 7 cut(s) 26, 43, 253, 288, 371, 1036, 1102
RsaNI GTAC 7 cut(s) 25, 42, 252, 287, 370, 1035, 1101
SapI GCTCTTC 2 cut(s) 82, 1269
SaqAI TTAA 2 cut(s) 153, 908
Sau3AI GATC 4 cut(s) 34, 520, 595, 1194
Sau96I GGNCC 5 cut(s) 96, 442, 704, 1304, 1468
SchI GAGTC 3 cut(s) 119, 419, 694
ScrFI CCNGG 4 cut(s) 39, 180, 458, 654
SduI GDGCHC 1 cut(s) 922
SfaNI GCATC 5 cut(s) 60, 385, 421, 847, 895
SfoI GGCGCC 1 cut(s) 1495
Sfr274I CTCGAG 2 cut(s) 881, 1321
SgrAI CRCCGGYG 1 cut(s) 1490
SinI GGWCC 3 cut(s) 96, 442, 1304
SlaI CTCGAG 2 cut(s) 881, 1321
SmlI CTYRAG 5 cut(s) 116, 191, 881, 907, 1321
SmoI CTYRAG 5 cut(s) 116, 191, 881, 907, 1321
SphI GCATGC 1 cut(s) 533
Sse9I AATT 9 cut(s) 17, 137, 400, 712, 838, 875, 1008, 1029, 1371
SsiI CCGC 8 cut(s) 74, 163, 233, 280, 283, 1339, 1471, 1474
SspDI GGCGCC 1 cut(s) 1493
SspI AATATT 1 cut(s) 673
SspMI CTAG 6 cut(s) 93, 225, 270, 642, 861, 1185
StyD4I CCNGG 4 cut(s) 37, 178, 456, 652
StyI CCWWGG 2 cut(s) 1091, 1529
TaaI ACNGT 4 cut(s) 14, 251, 472, 1232
TaiI ACGT 3 cut(s) 807, 1179, 1206
TaqI TCGA 4 cut(s) 683, 882, 1126, 1322
TasI AATT 9 cut(s) 17, 137, 400, 712, 838, 875, 1008, 1029, 1371
TatI WGTACW 2 cut(s) 369, 1100
TauI GCSGC 4 cut(s) 76, 166, 283, 1476
TfiI GAWTC 9 cut(s) 56, 215, 326, 347, 638, 740, 754, 812, 1045
Tru1I TTAA 2 cut(s) 153, 908
Tru9I TTAA 2 cut(s) 153, 908
TscAI CASTG 3 cut(s) 551, 568, 1318
TseFI GTSAC 3 cut(s) 111, 1486, 1536
TseI GCWGC 6 cut(s) 221, 235, 823, 1210, 1361, 1364
Tsp45I GTSAC 3 cut(s) 111, 1486, 1536
TspDTI ATGAA 6 cut(s) 351, 371, 603, 615, 1275, 1287
TspGWI ACGGA 2 cut(s) 922, 1120
TspRI CASTG 3 cut(s) 551, 568, 1318
Vha464I CTTAAG 1 cut(s) 907
VpaK11BI GGWCC 3 cut(s) 96, 442, 1304
VspI ATTAAT 1 cut(s) 153
XapI RAATTY 3 cut(s) 17, 400, 838
XbaI TCTAGA 1 cut(s) 269
XceI RCATGY 1 cut(s) 533
XhoI CTCGAG 2 cut(s) 881, 1321
XspI CTAG 6 cut(s) 93, 225, 270, 642, 861, 1185
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.