Rorug02G0014100

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009476
Physical Location & Seq
Forward (+)
1210391 .. 1211572
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0014100.1

Sequence Viewer

Length: 1086 bp
ATGATCACGACCGGAAGTGCGGCAGAGTCCTTGTCGGAAAACCCTCCAGTGGTGAAATCAGGCTATTATCCACCTTCGGATAATTTCCCACCTTCAGCCATAGACACTAGTTTCTTTACTCACGTCTACTATGCCTTTCTTGTGCCGAGCAACGTTACATTTAAATTTGAGCTGTCAAATGAAACGGCTTTACTCCTCTCGAACTTCACCACCACCCTCCGCCATAAATCTCCGCCGGTGAAGACCATGTTGTCCATGGGTGGTGGTGCCGGAGAAGAAGTGGCACACATCTTTGCTAGCATGGCCTCAAACCCGTCCTCACGGTCAAACTTCATTGACTCGGCCATAGAAGTTGCACGTAAGTTTGGGTTTGATGGGCTGGACCTGGACTGGGAATACCCTGAAAGCCCAAAAGAAATGAAGGCCTTGGGCCACTTGCTAAAACAATGGCGCCGCGAAATCAAGAAAGAGGCCCAGGCCACAAATAGCCAACCTCTACTGCTCACTGCCGCTGTCTACTTCTCCGCTGAGTTCTTCTTGGACGCCGTCATCCGCTCGTACCCGATTAAGTACATGGAAAAGTACTTGGATTGGGCCAATGTAATGTGCTTCGACTATCACGGGGCTTGGAGCAATATTACAGGGCCCAATGCACCATTATTTGACCCAAATAGTAATGTGAACTCGGTTTACGGGCTTAGGTCATGGATCAACGGTGGCATGACCCCAAACAAGCTCGTAATGGGCTTGCCACTCTATGGCCGGACATGGGAGCTCAAAGACCCTAAAGATAACGGTATTGGATCCATTGCTATAGGCCCAGGCCCAGGAAATGGTTCACTTGGCTTCAACGAGTTGGAGGCCCTAAATGACGAGTCGGGCGCCACCGTGGTATATGACGCCGACACCGTATCGGTATATTCTGTTAATGGGTCGAGTTGGATTGCATTTGATGATGCCTTTACAACAACCTCCAAGATAGGGTTTGCTCAAGCTCTTGGGCTTCGTGGGTACTTCTTTTGGGCCATCGTTCATGATAGCAATTGGAAGATCTCGGCCCAAGCTTCAAAATCATGGATTCTTTGA

Protein Analysis

361

Amino Acids

39.69

Weight (kDa)

5.21

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 21 - 346 2.2e-67 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 250
AccB1I GGYRCC 3 cut(s) 266, 450, 881
AccB7I CCANNNNNTGG 2 cut(s) 758, 833
AccBSI CCGCTC 1 cut(s) 555
AccI GTMKAC 2 cut(s) 126, 516
AccII CGCG 1 cut(s) 456
AciI CCGC 7 cut(s) 20, 220, 233, 454, 510, 525, 553
AclI AACGTT 1 cut(s) 153
AclWI GGATC 3 cut(s) 716, 798, 811
AcoI YGGCCR 2 cut(s) 342, 760
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 4 cut(s) 451, 543, 882, 900
AfaI GTAC 4 cut(s) 560, 572, 584, 1013
AfiI CCNNNNNNNGG 7 cut(s) 49, 391, 758, 769, 827, 833, 981
AgsI TTSAA 2 cut(s) 850, 1068
AhlI ACTAGT 1 cut(s) 107
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 4 cut(s) 384, 474, 820, 826
AluBI AGCT 5 cut(s) 172, 736, 775, 995, 1064
AluI AGCT 5 cut(s) 172, 736, 775, 995, 1064
Alw21I GWGCWC 1 cut(s) 777
AlwI GGATC 3 cut(s) 716, 798, 811
ApaI GGGCCC 1 cut(s) 648
ApoI RAATTY 1 cut(s) 164
Asp700I GAANNNNTTC 1 cut(s) 835
AspLEI GCGC 2 cut(s) 453, 884
AsuHPI GGTGA 3 cut(s) 64, 199, 250
AsuNHI GCTAGC 1 cut(s) 296
AvaII GGWCC 1 cut(s) 382
BaeGI GKGCMC 1 cut(s) 648
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 3 cut(s) 266, 450, 881
BanII GRGCYC 2 cut(s) 648, 777
BbsI GAAGAC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 777
BccI CCATC 2 cut(s) 368, 1034
BceAI ACGGC 2 cut(s) 201, 530
BciT130I CCWGG 4 cut(s) 386, 476, 822, 828
BclI TGATCA 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 2 cut(s) 108, 297
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 2 cut(s) 454, 885
BglII AGATCT 1 cut(s) 1050
BisI GCNGC 3 cut(s) 21, 454, 510
BlsI GCNGC 3 cut(s) 22, 455, 511
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 4 cut(s) 386, 476, 822, 828
Bme18I GGWCC 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 124
BmiI GGNNCC 5 cut(s) 268, 452, 646, 805, 883
BmrFI CCNGG 4 cut(s) 386, 476, 822, 828
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 1 cut(s) 946
BmtI GCTAGC 1 cut(s) 300
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 359
BsaHI GRCGYC 4 cut(s) 451, 543, 882, 900
BsaJI CCNNGG 6 cut(s) 255, 426, 474, 820, 826, 888
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 391, 758, 769, 827, 833, 981
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 47, 395
Bse3DI GCAATG 1 cut(s) 807
BseBI CCWGG 4 cut(s) 386, 476, 822, 828
BseDI CCNNGG 6 cut(s) 255, 426, 474, 820, 826, 888
BseGI GGATG 1 cut(s) 549
BseLI CCNNNNNNNGG 7 cut(s) 49, 391, 758, 769, 827, 833, 981
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 2 cut(s) 47, 395
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 456
Bsh1285I CGRYCG 1 cut(s) 12
BshNI GGYRCC 3 cut(s) 266, 450, 881
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 777
BsiSI CCGG 4 cut(s) 12, 236, 270, 763
BslI CCNNNNNNNGG 7 cut(s) 49, 391, 758, 769, 827, 833, 981
Bsp120I GGGCCC 1 cut(s) 644
Bsp1286I GDGCHC 2 cut(s) 648, 777
Bsp143I GATC 4 cut(s) 3, 708, 803, 1050
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 7 cut(s) 20, 220, 233, 454, 510, 525, 553
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 5 cut(s) 268, 452, 646, 805, 883
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 3 cut(s) 716, 798, 811
BspT107I GGYRCC 3 cut(s) 266, 450, 881
BsrBI CCGCTC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 2 cut(s) 47, 395
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 6 cut(s) 255, 426, 474, 820, 826, 888
BssMI GATC 4 cut(s) 3, 708, 803, 1050
BssNI GRCGYC 4 cut(s) 451, 543, 882, 900
BssT1I CCWWGG 2 cut(s) 255, 426
Bst2UI CCWGG 4 cut(s) 386, 476, 822, 828
Bst4CI ACNGT 5 cut(s) 324, 716, 797, 889, 910
BstACI GRCGYC 4 cut(s) 451, 543, 882, 900
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 298, 749
BstDEI CTNAG 2 cut(s) 528, 698
BstDSI CCRYGG 2 cut(s) 255, 888
BstF5I GGATG 1 cut(s) 549
BstFNI CGCG 1 cut(s) 456
BstH2I RGCGCY 2 cut(s) 454, 885
BstHHI GCGC 2 cut(s) 453, 884
BstKTI GATC 4 cut(s) 6, 711, 806, 1053
BstMBI GATC 4 cut(s) 3, 708, 803, 1050
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 4 cut(s) 386, 476, 822, 828
BstSCI CCNGG 4 cut(s) 384, 474, 820, 826
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 648
BstUI CGCG 1 cut(s) 456
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 2 cut(s) 803, 1050
BstYI RGATCY 2 cut(s) 803, 1050
BtgI CCRYGG 2 cut(s) 255, 888
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 1 cut(s) 549
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 54, 504
Cac8I GCNNGC 2 cut(s) 298, 749
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 2 cut(s) 453, 884
Cfr10I RCCGGY 1 cut(s) 235
CseI GACGC 2 cut(s) 551, 908
Csp6I GTAC 4 cut(s) 559, 571, 583, 1012
CviAII CATG 9 cut(s) 247, 256, 301, 574, 705, 721, 768, 1034, 1074
CviQI GTAC 4 cut(s) 559, 571, 583, 1012
DdeI CTNAG 2 cut(s) 528, 698
DinI GGCGCC 2 cut(s) 452, 883
DpnI GATC 4 cut(s) 5, 710, 805, 1052
DpnII GATC 4 cut(s) 3, 708, 803, 1050
DraI TTTAAA 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 2 cut(s) 342, 760
EciI GGCGGA 2 cut(s) 209, 222
Ecl136II GAGCTC 1 cut(s) 775
Eco130I CCWWGG 2 cut(s) 255, 426
Eco147I AGGCCT 1 cut(s) 425
Eco24I GRGCYC 2 cut(s) 648, 777
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 775
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 775
EcoO109I RGGNCCY 2 cut(s) 644, 862
EcoRII CCWGG 4 cut(s) 384, 474, 820, 826
EcoT14I CCWWGG 2 cut(s) 255, 426
EcoT38I GRGCYC 2 cut(s) 648, 777
EgeI GGCGCC 2 cut(s) 452, 883
EheI GGCGCC 2 cut(s) 452, 883
ErhI CCWWGG 2 cut(s) 255, 426
FaeI CATG 9 cut(s) 250, 259, 304, 577, 708, 724, 771, 1037, 1077
FatI CATG 9 cut(s) 246, 255, 300, 573, 704, 720, 767, 1033, 1073
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 126, 516
Fnu4HI GCNGC 3 cut(s) 21, 454, 510
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 2 cut(s) 648, 777
Fsp4HI GCNGC 3 cut(s) 21, 454, 510
FspBI CTAG 2 cut(s) 108, 297
GlaI GCGC 2 cut(s) 452, 883
GluI GCNGC 3 cut(s) 21, 454, 510
GsuI CTGGAG 1 cut(s) 30
HaeII RGCGCY 2 cut(s) 454, 885
HapII CCGG 4 cut(s) 12, 236, 270, 763
HgaI GACGC 2 cut(s) 551, 908
HhaI GCGC 2 cut(s) 453, 884
Hin1I GRCGYC 4 cut(s) 451, 543, 882, 900
Hin1II CATG 9 cut(s) 250, 259, 304, 577, 708, 724, 771, 1037, 1077
Hin6I GCGC 2 cut(s) 451, 882
HinP1I GCGC 2 cut(s) 451, 882
HindIII AAGCTT 1 cut(s) 1062
HinfI GANTC 4 cut(s) 26, 338, 875, 1078
HpaII CCGG 4 cut(s) 12, 236, 270, 763
HphI GGTGA 3 cut(s) 64, 199, 250
Hpy166II GTNNAC 5 cut(s) 127, 517, 682, 691, 839
Hpy188I TCNGA 2 cut(s) 37, 79
Hpy188III TCNNGA 4 cut(s) 7, 199, 463, 1034
Hpy8I GTNNAC 5 cut(s) 127, 517, 682, 691, 839
HpyAV CCTTC 3 cut(s) 84, 102, 415
HpyCH4III ACNGT 5 cut(s) 324, 716, 797, 889, 910
HpyCH4IV ACGT 3 cut(s) 123, 153, 358
HpyCH4V TGCA 3 cut(s) 356, 653, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 2 cut(s) 528, 698
HpySE526I ACGT 3 cut(s) 123, 153, 358
Hsp92I GRCGYC 4 cut(s) 451, 543, 882, 900
Hsp92II CATG 9 cut(s) 250, 259, 304, 577, 708, 724, 771, 1037, 1077
HspAI GCGC 2 cut(s) 451, 882
KasI GGCGCC 2 cut(s) 450, 881
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 708, 803, 1050
LmnI GCTCC 2 cut(s) 630, 772
LweI GCATC 1 cut(s) 946
MaeI CTAG 2 cut(s) 108, 297
MaeII ACGT 3 cut(s) 123, 153, 358
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 5, 710, 805, 1052
MbiI CCGCTC 1 cut(s) 555
MboI GATC 4 cut(s) 3, 708, 803, 1050
MboII GAAGA 4 cut(s) 253, 287, 526, 1060
MfeI CAATTG 1 cut(s) 1042
MflI RGATCY 2 cut(s) 803, 1050
MhlI GDGCHC 2 cut(s) 648, 777
MluCI AATT 3 cut(s) 82, 164, 1042
Mly113I GGCGCC 2 cut(s) 451, 882
MlyI GAGTC 3 cut(s) 35, 332, 884
MmeI TCCRAC 3 cut(s) 15, 837, 920
MnlI CCTC 9 cut(s) 54, 206, 227, 316, 328, 463, 504, 853, 982
MroXI GAANNNNTTC 1 cut(s) 835
MseI TTAA 3 cut(s) 162, 567, 927
MspA1I CMGCKG 2 cut(s) 512, 527
MspI CCGG 4 cut(s) 12, 236, 270, 763
MspR9I CCNGG 4 cut(s) 386, 476, 822, 828
MunI CAATTG 1 cut(s) 1042
MvaI CCWGG 4 cut(s) 386, 476, 822, 828
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 1 cut(s) 302
NarI GGCGCC 2 cut(s) 451, 882
NcoI CCATGG 1 cut(s) 255
NdeII GATC 4 cut(s) 3, 708, 803, 1050
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 9 cut(s) 250, 259, 304, 577, 708, 724, 771, 1037, 1077
NlaIV GGNNCC 5 cut(s) 268, 452, 646, 805, 883
NmeAIII GCCGAG 3 cut(s) 171, 320, 1034
PagI TCATGA 1 cut(s) 1033
PceI AGGCCT 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 906
PdmI GAANNNNTTC 1 cut(s) 835
PfeI GAWTC 1 cut(s) 1078
PflFI GACNNNGTC 1 cut(s) 545
PflMI CCANNNNNTGG 2 cut(s) 758, 833
PkrI GCNGC 3 cut(s) 22, 455, 511
PleI GAGTC 3 cut(s) 34, 332, 883
PluTI GGCGCC 2 cut(s) 454, 885
PpsI GAGTC 3 cut(s) 34, 332, 883
Ppu21I YACGTR 1 cut(s) 359
Psp124BI GAGCTC 1 cut(s) 777
Psp1406I AACGTT 1 cut(s) 153
Psp6I CCWGG 4 cut(s) 384, 474, 820, 826
PspGI CCWGG 4 cut(s) 384, 474, 820, 826
PspN4I GGNNCC 5 cut(s) 268, 452, 646, 805, 883
PspOMI GGGCCC 1 cut(s) 644
PsrI GAACNNNNNNTAC 2 cut(s) 674, 706
PsuI RGATCY 2 cut(s) 803, 1050
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 4 cut(s) 560, 572, 584, 1013
RsaNI GTAC 4 cut(s) 559, 571, 583, 1012
SacI GAGCTC 1 cut(s) 777
SaqAI TTAA 3 cut(s) 162, 567, 927
SatI GCNGC 3 cut(s) 21, 454, 510
Sau3AI GATC 4 cut(s) 3, 708, 803, 1050
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 3 cut(s) 35, 332, 884
ScrFI CCNGG 4 cut(s) 386, 476, 822, 828
SduI GDGCHC 2 cut(s) 648, 777
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 2 cut(s) 452, 883
SgrAI CRCCGGYG 1 cut(s) 235
SinI GGWCC 1 cut(s) 382
SmiI ATTTAAAT 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 990
SmoI CTYRAG 1 cut(s) 990
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 3 cut(s) 82, 164, 1042
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 7 cut(s) 20, 220, 233, 454, 510, 525, 553
SspDI GGCGCC 2 cut(s) 450, 881
SspI AATATT 1 cut(s) 637
SspMI CTAG 2 cut(s) 108, 297
SstI GAGCTC 1 cut(s) 777
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 4 cut(s) 384, 474, 820, 826
StyI CCWWGG 2 cut(s) 255, 426
SwaI ATTTAAAT 1 cut(s) 163
TaaI ACNGT 5 cut(s) 324, 716, 797, 889, 910
TaiI ACGT 3 cut(s) 126, 156, 361
TaqI TCGA 3 cut(s) 200, 612, 935
TasI AATT 3 cut(s) 82, 164, 1042
TatI WGTACW 2 cut(s) 570, 582
TauI GCSGC 3 cut(s) 23, 456, 512
TfiI GAWTC 1 cut(s) 1078
Tru1I TTAA 3 cut(s) 162, 567, 927
Tru9I TTAA 3 cut(s) 162, 567, 927
TscAI CASTG 2 cut(s) 54, 511
TspDTI ATGAA 4 cut(s) 195, 322, 434, 1022
TspRI CASTG 2 cut(s) 54, 511
Tth111I GACNNNGTC 1 cut(s) 545
Van91I CCANNNNNTGG 2 cut(s) 758, 833
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmiI GTMKAC 2 cut(s) 126, 516
XmnI GAANNNNTTC 1 cut(s) 835
XspI CTAG 2 cut(s) 108, 297
ZrmI AGTACT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.