RchiOBHm_Chr2g0091181

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009476
Physical Location & Seq
Forward (+)
4855254 .. 4855895
642 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46638

Sequence Viewer

Length: 642 bp
ATGCACCAATGGAGACATGTTACTGAAACTGATGGTCCACTTCGTTGGGGAATGAAGGGAAGGAACTCCCATGATATCTTATCTAACTTCGATGATCCAGACATGATGCCTTATGTTACAAATTCTGGTGATTATCTTGATGCACTAGGCTTTGAAGAATTGCTGGAGCATCTTGCTCAGGCAGAGAGATTAAGACTAGGGGCACCTCCTGCTGCTGTCTCCTTTTTGAATAATCTGCCTCTCATTCTTATTAGTAAGGAACATGGGAAGCATGATGACTTAGCCTGTGCAATTTGCAAGGATGTATTGACTATTAGCACTGCAATGAATCGGCTTCCTTGCTTTCACTTGTATCACACTTCCTGTATTCTGCCATGGTTGAGTACACGAAATTCTTGCCCCCTTTGTCGTTATAAGATACCAGCTGATGACAGAGACTACGAAGAGGGGAAGCGAAATGCTGGTGGTAGAGTGGAGATCCACACCCGGCGGCAGAATGCTAGAGATGACAGGTCTGCTGGTGTTTTTAATAGAGCTGAAGAAGGCGAAGATCATGCTTTTAGTGAAACTAGAGTTAAGTATAGGTTCCAAGTAGGTAGGGAATCTGACTCTAATACCTCCGGTGGAGAAAATAGTCAATGA

Protein Analysis

213

Amino Acids

24.17

Weight (kDa)

5.92

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 95 - 137 7.5e-11 Ring finger domain
zf-C3HC4_2 PF13923 96 - 136 3.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 96 - 136 9.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 109 - 137 2.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 202
AciI CCGC 1 cut(s) 490
AclWI GGATC 2 cut(s) 89, 472
AcsI RAATTY 2 cut(s) 121, 391
AcuI CTGAAG 1 cut(s) 558
AfaI GTAC 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 2 cut(s) 155, 229
AluBI AGCT 2 cut(s) 425, 536
AluI AGCT 2 cut(s) 425, 536
Alw26I GTCTC 3 cut(s) 7, 223, 429
AlwI GGATC 2 cut(s) 89, 472
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 2 cut(s) 121, 391
AspS9I GGNCC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 199
BccI CCATC 1 cut(s) 26
BcgI CGANNNNNNTGC 4 cut(s) 378, 412, 536, 570
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 7, 223, 429
BfaI CTAG 4 cut(s) 146, 197, 501, 570
BisI GCNGC 2 cut(s) 213, 491
BlsI GCNGC 2 cut(s) 214, 492
Bme1390I CCNGG 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 2 cut(s) 204, 587
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 3 cut(s) 96, 130, 178
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 620
Bse3DI GCAATG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 1 cut(s) 307
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 202
BsiSI CCGG 2 cut(s) 487, 621
BsmAI GTCTC 3 cut(s) 7, 223, 429
BsmI GAATGC 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 3 cut(s) 94, 477, 550
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 1 cut(s) 490
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 204, 587
BspPI GGATC 2 cut(s) 89, 472
BspT107I GGYRCC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 3 cut(s) 94, 477, 550
BssT1I CCWWGG 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 177, 280
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 3 cut(s) 97, 480, 553
BstMAI GTCTC 3 cut(s) 7, 223, 429
BstMBI GATC 3 cut(s) 94, 477, 550
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 485
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 477
BstXI CCANNNNNNTGG 1 cut(s) 45
BstYI RGATCY 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 384
CviAII CATG 7 cut(s) 17, 71, 103, 263, 272, 375, 554
CviJI RGCY 5 cut(s) 150, 284, 334, 425, 536
CviKI_1 RGCY 5 cut(s) 150, 284, 334, 425, 536
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 177, 280
DpnI GATC 3 cut(s) 96, 479, 552
DpnII GATC 3 cut(s) 94, 477, 550
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 374
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 558
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 7 cut(s) 20, 74, 106, 266, 275, 378, 557
FatI CATG 7 cut(s) 16, 70, 102, 262, 271, 374, 553
Fnu4HI GCNGC 2 cut(s) 213, 491
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 213, 491
FspBI CTAG 4 cut(s) 146, 197, 501, 570
GluI GCNGC 2 cut(s) 213, 491
GsuI CTGGAG 1 cut(s) 185
HapII CCGG 2 cut(s) 487, 621
Hin1II CATG 7 cut(s) 20, 74, 106, 266, 275, 378, 557
HinfI GANTC 3 cut(s) 328, 602, 608
HpaII CCGG 2 cut(s) 487, 621
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 38, 386
Hpy188I TCNGA 1 cut(s) 607
Hpy188III TCNNGA 2 cut(s) 98, 137
Hpy8I GTNNAC 2 cut(s) 38, 386
HpyAV CCTTC 3 cut(s) 49, 54, 536
HpyCH4V TGCA 5 cut(s) 4, 143, 290, 297, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 2 cut(s) 177, 280
Hsp92II CATG 7 cut(s) 20, 74, 106, 266, 275, 378, 557
Kzo9I GATC 3 cut(s) 94, 477, 550
LmnI GCTCC 1 cut(s) 166
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 3 cut(s) 96, 130, 178
MaeI CTAG 4 cut(s) 146, 197, 501, 570
MaeIII GTNAC 2 cut(s) 19, 115
MalI GATC 3 cut(s) 96, 479, 552
MboI GATC 3 cut(s) 94, 477, 550
MboII GAAGA 4 cut(s) 167, 455, 551, 560
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 4 cut(s) 121, 158, 291, 391
MlyI GAGTC 1 cut(s) 602
MnlI CCTC 4 cut(s) 216, 249, 439, 628
MseI TTAA 3 cut(s) 191, 528, 576
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 2 cut(s) 487, 621
MspR9I CCNGG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 502
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 487
NcoI CCATGG 1 cut(s) 374
NdeII GATC 3 cut(s) 94, 477, 550
NlaIII CATG 7 cut(s) 20, 74, 106, 266, 275, 378, 557
NlaIV GGNNCC 2 cut(s) 204, 587
NspI RCATGY 1 cut(s) 20
PciI ACATGT 1 cut(s) 16
PctI GAATGC 1 cut(s) 502
PfeI GAWTC 2 cut(s) 328, 602
PkrI GCNGC 2 cut(s) 214, 492
PleI GAGTC 1 cut(s) 602
PpsI GAGTC 1 cut(s) 602
PscI ACATGT 1 cut(s) 16
PsiI TTATAA 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 204, 587
PspPI GGNCC 1 cut(s) 35
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 3 cut(s) 191, 528, 576
SatI GCNGC 2 cut(s) 213, 491
Sau3AI GATC 3 cut(s) 94, 477, 550
Sau96I GGNCC 1 cut(s) 35
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 487
SduI GDGCHC 1 cut(s) 205
SetI ASST 7 cut(s) 208, 427, 515, 538, 587, 598, 620
SfaNI GCATC 3 cut(s) 96, 130, 178
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 4 cut(s) 121, 158, 291, 391
SsiI CCGC 1 cut(s) 490
SspMI CTAG 4 cut(s) 146, 197, 501, 570
StyD4I CCNGG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 374
TaqI TCGA 1 cut(s) 90
TasI AATT 4 cut(s) 121, 158, 291, 391
TatI WGTACW 1 cut(s) 383
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 2 cut(s) 328, 602
Tru1I TTAA 3 cut(s) 191, 528, 576
Tru9I TTAA 3 cut(s) 191, 528, 576
TscAI CASTG 1 cut(s) 325
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 68, 341
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 2 cut(s) 121, 391
XceI RCATGY 1 cut(s) 20
XspI CTAG 4 cut(s) 146, 197, 501, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.