pycom04g15740

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009476
Physical Location & Seq
Forward (+)
18534320 .. 18535867
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15740.1

Sequence Viewer

Length: 1548 bp
ATGTGTGGGGACTGTAAATTTCTTTACCTTGAAGATCTTGGTTCCCCCTCACATGATTCTTATCAGAGGACGCCGCCTACAAGAAGAAGAGCTAGGACTGGGAGTTCCGAGTCACTTGAGAATACTTTTTCACAACAATTCTCGCATATGATTAATCTGGTGCGGCAGAACCAATCCCCTGGCTCTGGGATTGAGGAACTACCTGTGGGTGGTGATTCTGCTGCTAGGATACCGCTGCGCACAAGTAACCGTACTACACCAAGTGGGTCTAGAAGATGGCGGCGGGTGCTCTCTGATACTGAAAGTGAGGGTTACGAAAATGTGGATTCTTTCTATGGAGAAACTGAATCAAATCTCAGTTTTGGTCGGTACAGGGTATTTCATGGTGAGAGCGATGCAATTTCTTTAAGTGCATATGGAGGGGATTCTGATGCTTCTGTGGACCTACATGGTTTCCTGGATACTGAAACGATTATTCCACCGGATGATGGAAGTGCTTATGATAGTGATACTGACATTGATCCCATGCATGCTGGTCTTAGCCAGTGGAACTCAGATGACACTGAGGACGATGATGATGAGGGGGATGAAGAAGATGATGAGTGGGAAGAAGCCGACGTTGAAGTAAACATGGTTGAATCTAGAGAAACCCCAGGTGGCCGGCTTCGTAATCTTTTAGTTTCACGTCCAGGCAGAAGTAATGGACCATCCATTGGGCGCCACCATACATATTTTCCTGATTCTGAGGGTACGTTTCATTGGAGAATGAGGAGGAGGAGTCAAATATATACCCGTACCCTCTTTTCTAACGTGGACGAATCAGAGTTGCTGCCTTATGATGGAAATTCTGGTGATTATCTTGATGCTGGAGGCTTTGAAGAATTGCTCGAGCATCTTGCTGAGGTTGATAGCTTGAGACGAGGAGCTCCTCCTGCTTCTGCGTCCTTTGTGAATAATCTGCCTCGTGTTGTCATTAGCAAGGAACATGACAAGCATGATGACTTAGCCTGTGCAATTTGCAAAGATGTATTGACAATTGGTACTGAAGTGAATAAGCTTCCCTGCTCTCACCTCTATCACTCTTTGTGTATTTTGCCATGGTTGAGTACACGGAATACTTGTCCACTCTGTCGATATGAGCTACCGACTGATGACAGAGACTACGAAGAGGGGAAACGGAACGTTAGTGCTAGAGCAGAGATCCACAATGTCCGGCAGCAGAATGCAAGTGTGGACAGTTCCTCCGGTGCTTTCAACAGAGCTTATGAAGATGAGGAGCATGAGCAGAGTACAGCAAATAGGGAACCAACCACTAATGGCTCGAGAGGAGAAAATAGCCGGGGGAGATGGTTTCTTCTCGCAGCTGCTCCAATTGTCAGTCTTGTGGGTATTGTTCTTGTGCTGTGGTTAGGAAGTCCGCTGTTGGAAAGAAGAGGTCTGGCAGGAAACCAGAACTTCGCCAGTCAGGCCCGCCACCAGAGTCCTATCACCAGCGCCTCACCCAACCACAGGGAGAACAGAAGCCGGAGATGGTGGTCACTTTTCTGA

Protein Analysis

516

Amino Acids

57.7

Weight (kDa)

4.91

Isoelectric Point (pI)

60.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 336 - 378 1.7e-11 Ring finger domain
zf-C3HC4_2 PF13923 337 - 377 4.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 337 - 364 6e-07 RING-like zinc finger
zf-C3HC4 PF00097 337 - 377 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 348 - 378 7.3e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 717
AccB7I CCANNNNNTGG 1 cut(s) 713
AciI CCGC 7 cut(s) 74, 163, 233, 280, 283, 1418, 1471
AclI AACGTT 1 cut(s) 1182
AclWI GGATC 2 cut(s) 515, 1195
AcoI YGGCCR 1 cut(s) 658
AcsI RAATTY 2 cut(s) 17, 844
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 2 cut(s) 71, 718
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 7 cut(s) 253, 371, 751, 796, 1042, 1108, 1291
AfiI CCNNNNNNNGG 6 cut(s) 185, 209, 488, 713, 839, 1509
AgsI TTSAA 5 cut(s) 32, 623, 638, 878, 1255
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 4 cut(s) 178, 456, 652, 688
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
AluBI AGCT 7 cut(s) 92, 912, 926, 1057, 1141, 1262, 1364
AluI AGCT 7 cut(s) 92, 912, 926, 1057, 1141, 1262, 1364
Alw21I GWGCWC 2 cut(s) 291, 928
Alw26I GTCTC 2 cut(s) 910, 1152
AlwI GGATC 2 cut(s) 515, 1195
Ama87I CYCGRG 2 cut(s) 887, 1321
AoxI GGCC 2 cut(s) 658, 1467
ApeKI GCWGC 6 cut(s) 221, 235, 829, 1216, 1361, 1364
ApoI RAATTY 2 cut(s) 17, 844
AseI ATTAAT 1 cut(s) 153
AspLEI GCGC 3 cut(s) 240, 720, 1496
AspS9I GGNCC 3 cut(s) 442, 704, 1468
AsuC2I CCSGG 1 cut(s) 1340
AsuHPI GGTGA 6 cut(s) 224, 398, 863, 1061, 1480, 1491
AvaI CYCGRG 2 cut(s) 887, 1321
AvaII GGWCC 2 cut(s) 442, 704
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BanI GGYRCC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 928
BauI CACGAG 1 cut(s) 963
Bbv12I GWGCWC 2 cut(s) 291, 928
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 6 cut(s) 208, 222, 816, 1228, 1351, 1373
BccI CCATC 6 cut(s) 270, 482, 715, 833, 1341, 1524
BcgI CGANNNNNNTGC 2 cut(s) 878, 912
BciT130I CCWGG 4 cut(s) 180, 458, 654, 690
BciVI GTATCC 2 cut(s) 222, 454
BcnI CCSGG 1 cut(s) 1340
BcoDI GTCTC 2 cut(s) 910, 1152
BfaI CTAG 5 cut(s) 93, 225, 270, 642, 1191
BfoI RGCGCY 2 cut(s) 721, 1497
BfuI GTATCC 2 cut(s) 222, 454
BglI GCCNNNNNGGC 1 cut(s) 1466
BglII AGATCT 1 cut(s) 34
BisI GCNGC 9 cut(s) 74, 164, 222, 236, 281, 830, 1217, 1362, 1365
BlsI GCNGC 9 cut(s) 75, 165, 223, 237, 282, 831, 1218, 1363, 1366
Bme1390I CCNGG 5 cut(s) 180, 458, 654, 690, 1340
Bme18I GGWCC 2 cut(s) 442, 704
BmeT110I CYCGRG 2 cut(s) 887, 1321
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 3 cut(s) 442, 704, 1468
BmiI GGNNCC 3 cut(s) 43, 719, 1305
BmrFI CCNGG 5 cut(s) 180, 458, 654, 690, 1340
BmrI ACTGGG 1 cut(s) 108
BmsI GCATC 4 cut(s) 385, 421, 853, 901
BmuI ACTGGG 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 888
Bpu10I CCTNAGC 1 cut(s) 900
BpuEI CTTGAG 2 cut(s) 137, 934
BpuMI CCSGG 1 cut(s) 1340
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 2 cut(s) 71, 718
BsaJI CCNNGG 4 cut(s) 178, 652, 1097, 1339
BsaWI WCCGGW 2 cut(s) 481, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 763, 793, 1226, 1256
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 209, 488, 713, 839, 1509
Bse118I RCCGGY 1 cut(s) 660
Bse1I ACTGG 3 cut(s) 103, 544, 1461
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 4 cut(s) 180, 458, 654, 690
BseDI CCNNGG 4 cut(s) 178, 652, 1097, 1339
BseGI GGATG 3 cut(s) 490, 592, 707
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 6 cut(s) 185, 209, 488, 713, 839, 1509
BseMII CTCAG 5 cut(s) 370, 555, 567, 735, 891
BseNI ACTGG 3 cut(s) 103, 544, 1461
BseRI GAGGAG 7 cut(s) 784, 787, 790, 918, 936, 1289, 1341
BseXI GCAGC 6 cut(s) 208, 222, 816, 1228, 1351, 1373
BshFI GGCC 2 cut(s) 660, 1469
BshNI GGYRCC 1 cut(s) 717
BsiHKAI GWGCWC 2 cut(s) 291, 928
BsiHKCI CYCGRG 2 cut(s) 887, 1321
BsiSI CCGG 6 cut(s) 482, 661, 1213, 1245, 1339, 1525
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 6 cut(s) 185, 209, 488, 713, 839, 1509
BsmAI GTCTC 2 cut(s) 910, 1152
BsmBI CGTCTC 1 cut(s) 910
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 2 cut(s) 660, 1469
BsoBI CYCGRG 2 cut(s) 887, 1321
Bsp1286I GDGCHC 2 cut(s) 291, 928
Bsp143I GATC 3 cut(s) 34, 520, 1200
Bsp19I CCATGG 1 cut(s) 1097
BspACI CCGC 7 cut(s) 74, 163, 233, 280, 283, 1418, 1471
BspANI GGCC 2 cut(s) 660, 1469
BspCNI CTCAG 5 cut(s) 369, 556, 566, 736, 892
BspLI GGNNCC 3 cut(s) 43, 719, 1305
BspPI GGATC 2 cut(s) 515, 1195
BspQI GCTCTTC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 660
BsrI ACTGG 3 cut(s) 103, 544, 1461
BssAI RCCGGY 1 cut(s) 660
BssECI CCNNGG 4 cut(s) 178, 652, 1097, 1339
BssMI GATC 3 cut(s) 34, 520, 1200
BssNI GRCGYC 2 cut(s) 71, 718
BssSI CACGAG 1 cut(s) 963
BssT1I CCWWGG 1 cut(s) 1097
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 4 cut(s) 180, 458, 654, 690
Bst4CI ACNGT 3 cut(s) 14, 251, 1238
Bst6I CTCTTC 3 cut(s) 82, 1161, 1426
BstACI GRCGYC 2 cut(s) 71, 718
BstC8I GCNNGC 3 cut(s) 531, 662, 1471
BstDEI CTNAG 7 cut(s) 356, 539, 553, 564, 744, 900, 1003
BstDSI CCRYGG 1 cut(s) 1097
BstF5I GGATG 3 cut(s) 490, 592, 707
BstH2I RGCGCY 2 cut(s) 721, 1497
BstHHI GCGC 3 cut(s) 240, 720, 1496
BstKTI GATC 3 cut(s) 37, 523, 1203
BstMAI GTCTC 2 cut(s) 910, 1152
BstMBI GATC 3 cut(s) 34, 520, 1200
BstMWI GCNNNNNNNGC 3 cut(s) 286, 932, 1466
BstNI CCWGG 4 cut(s) 180, 458, 654, 690
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 5 cut(s) 178, 456, 652, 688, 1338
BstV1I GCAGC 6 cut(s) 208, 222, 816, 1228, 1351, 1373
BstX2I RGATCY 2 cut(s) 34, 1200
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 2 cut(s) 34, 1200
BsuI GTATCC 2 cut(s) 222, 454
BsuRI GGCC 2 cut(s) 660, 1469
BtgI CCRYGG 1 cut(s) 1097
BtgZI GCGATG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 3 cut(s) 490, 592, 707
BtsIMutI CAGTG 2 cut(s) 551, 561
Cac8I GCNNGC 3 cut(s) 531, 662, 1471
CfoI GCGC 3 cut(s) 240, 720, 1496
Cfr10I RCCGGY 1 cut(s) 660
Cfr13I GGNCC 3 cut(s) 442, 704, 1468
CseI GACGC 2 cut(s) 79, 930
Csp6I GTAC 7 cut(s) 252, 370, 750, 795, 1041, 1107, 1290
CviQI GTAC 7 cut(s) 252, 370, 750, 795, 1041, 1107, 1290
DdeI CTNAG 7 cut(s) 356, 539, 553, 564, 744, 900, 1003
DinI GGCGCC 1 cut(s) 719
DpnI GATC 3 cut(s) 36, 522, 1202
DpnII GATC 3 cut(s) 34, 520, 1200
DraIII CACNNNGTG 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 658
Eam1104I CTCTTC 3 cut(s) 82, 1161, 1426
EarI CTCTTC 3 cut(s) 82, 1161, 1426
Ecl136II GAGCTC 1 cut(s) 926
Eco130I CCWWGG 1 cut(s) 1097
Eco24I GRGCYC 1 cut(s) 928
Eco47I GGWCC 2 cut(s) 442, 704
Eco53kI GAGCTC 1 cut(s) 926
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 2 cut(s) 887, 1321
EcoICRI GAGCTC 1 cut(s) 926
EcoRII CCWGG 4 cut(s) 178, 456, 652, 688
EcoT14I CCWWGG 1 cut(s) 1097
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 928
EgeI GGCGCC 1 cut(s) 719
EheI GGCGCC 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 1097
Esp3I CGTCTC 1 cut(s) 910
FaqI GGGAC 1 cut(s) 23
FauI CCCGC 2 cut(s) 276, 1478
FauNDI CATATG 2 cut(s) 147, 415
Fnu4HI GCNGC 9 cut(s) 74, 164, 222, 236, 281, 830, 1217, 1362, 1365
FokI GGATG 3 cut(s) 497, 599, 694
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 9 cut(s) 74, 164, 222, 236, 281, 830, 1217, 1362, 1365
FspBI CTAG 5 cut(s) 93, 225, 270, 642, 1191
FspI TGCGCA 1 cut(s) 239
GlaI GCGC 3 cut(s) 239, 719, 1495
GluI GCNGC 9 cut(s) 74, 164, 222, 236, 281, 830, 1217, 1362, 1365
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 2 cut(s) 721, 1497
HaeIII GGCC 2 cut(s) 660, 1469
HapII CCGG 6 cut(s) 482, 661, 1213, 1245, 1339, 1525
HgaI GACGC 2 cut(s) 79, 930
HhaI GCGC 3 cut(s) 240, 720, 1496
Hin1I GRCGYC 2 cut(s) 71, 718
Hin6I GCGC 3 cut(s) 238, 718, 1494
HinP1I GCGC 3 cut(s) 238, 718, 1494
HindIII AAGCTT 1 cut(s) 1055
HpaII CCGG 6 cut(s) 482, 661, 1213, 1245, 1339, 1525
HphI GGTGA 6 cut(s) 224, 398, 863, 1061, 1480, 1491
Hpy166II GTNNAC 6 cut(s) 442, 628, 814, 1109, 1124, 1234
Hpy188I TCNGA 8 cut(s) 66, 109, 295, 430, 556, 745, 823, 1547
Hpy188III TCNNGA 5 cut(s) 270, 642, 737, 860, 1323
Hpy8I GTNNAC 6 cut(s) 442, 628, 814, 1109, 1124, 1234
Hpy99I CGWCG 1 cut(s) 620
HpyCH4III ACNGT 3 cut(s) 14, 251, 1238
HpyCH4IV ACGT 5 cut(s) 618, 685, 752, 810, 1182
HpyCH4V TGCA 6 cut(s) 398, 413, 529, 1013, 1020, 1226
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 932, 1466
HpyF3I CTNAG 7 cut(s) 356, 539, 553, 564, 744, 900, 1003
HpySE526I ACGT 5 cut(s) 618, 685, 752, 810, 1182
Hsp92I GRCGYC 2 cut(s) 71, 718
HspAI GCGC 3 cut(s) 238, 718, 1494
KasI GGCGCC 1 cut(s) 717
KroI GCCGGC 1 cut(s) 660
KroNI GCCGGC 1 cut(s) 662
Kzo9I GATC 3 cut(s) 34, 520, 1200
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 4 cut(s) 923, 931, 1276, 1372
Lsp1109I GCAGC 6 cut(s) 208, 222, 816, 1228, 1351, 1373
LweI GCATC 4 cut(s) 385, 421, 853, 901
MaeI CTAG 5 cut(s) 93, 225, 270, 642, 1191
MaeII ACGT 5 cut(s) 618, 685, 752, 810, 1182
MaeIII GTNAC 4 cut(s) 111, 245, 311, 1536
MalI GATC 3 cut(s) 36, 522, 1202
MboI GATC 3 cut(s) 34, 520, 1200
MfeI CAATTG 2 cut(s) 1035, 1371
MflI RGATCY 2 cut(s) 34, 1200
MhlI GDGCHC 2 cut(s) 291, 928
MluCI AATT 8 cut(s) 17, 137, 399, 844, 881, 1014, 1035, 1371
Mly113I GGCGCC 1 cut(s) 718
MlyI GAGTC 3 cut(s) 119, 787, 1489
MmeI TCCRAC 1 cut(s) 1404
Mph1103I ATGCAT 1 cut(s) 531
MroNI GCCGGC 1 cut(s) 660
MseI TTAA 2 cut(s) 153, 407
MspA1I CMGCKG 3 cut(s) 235, 1364, 1420
MspI CCGG 6 cut(s) 482, 661, 1213, 1245, 1339, 1525
MspR9I CCNGG 5 cut(s) 180, 458, 654, 690, 1340
MunI CAATTG 2 cut(s) 1035, 1371
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 4 cut(s) 180, 458, 654, 690
MwoI GCNNNNNNNGC 3 cut(s) 286, 932, 1466
NaeI GCCGGC 1 cut(s) 662
NarI GGCGCC 1 cut(s) 718
NciI CCSGG 1 cut(s) 1340
NcoI CCATGG 1 cut(s) 1097
NdeI CATATG 2 cut(s) 147, 415
NdeII GATC 3 cut(s) 34, 520, 1200
NgoMIV GCCGGC 1 cut(s) 660
NlaIV GGNNCC 3 cut(s) 43, 719, 1305
NmuCI GTSAC 2 cut(s) 111, 1536
NsbI TGCGCA 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PaeR7I CTCGAG 2 cut(s) 887, 1321
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 1 cut(s) 1228
PdiI GCCGGC 1 cut(s) 662
PfeI GAWTC 8 cut(s) 56, 215, 326, 347, 425, 638, 740, 818
PflMI CCANNNNNTGG 1 cut(s) 713
PfoI TCCNGGA 1 cut(s) 456
PkrI GCNGC 9 cut(s) 75, 165, 223, 237, 282, 831, 1218, 1363, 1366
PleI GAGTC 3 cut(s) 118, 786, 1488
PluTI GGCGCC 1 cut(s) 721
PpsI GAGTC 3 cut(s) 118, 786, 1488
PshBI ATTAAT 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 928
Psp1406I AACGTT 1 cut(s) 1182
Psp6I CCWGG 4 cut(s) 178, 456, 652, 688
PspGI CCWGG 4 cut(s) 178, 456, 652, 688
PspN4I GGNNCC 3 cut(s) 43, 719, 1305
PspPI GGNCC 3 cut(s) 442, 704, 1468
PspXI VCTCGAGB 1 cut(s) 887
PsuI RGATCY 2 cut(s) 34, 1200
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 7 cut(s) 253, 371, 751, 796, 1042, 1108, 1291
RsaNI GTAC 7 cut(s) 252, 370, 750, 795, 1041, 1107, 1290
SacI GAGCTC 1 cut(s) 928
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 153, 407
SatI GCNGC 9 cut(s) 74, 164, 222, 236, 281, 830, 1217, 1362, 1365
Sau3AI GATC 3 cut(s) 34, 520, 1200
Sau96I GGNCC 3 cut(s) 442, 704, 1468
SchI GAGTC 3 cut(s) 119, 787, 1489
ScrFI CCNGG 5 cut(s) 180, 458, 654, 690, 1340
SduI GDGCHC 2 cut(s) 291, 928
SfaNI GCATC 4 cut(s) 385, 421, 853, 901
SfoI GGCGCC 1 cut(s) 719
Sfr274I CTCGAG 2 cut(s) 887, 1321
SinI GGWCC 2 cut(s) 442, 704
SlaI CTCGAG 2 cut(s) 887, 1321
SmlI CTYRAG 4 cut(s) 116, 887, 913, 1321
SmoI CTYRAG 4 cut(s) 116, 887, 913, 1321
SphI GCATGC 1 cut(s) 533
Sse9I AATT 8 cut(s) 17, 137, 399, 844, 881, 1014, 1035, 1371
SsiI CCGC 7 cut(s) 74, 163, 233, 280, 283, 1418, 1471
SspDI GGCGCC 1 cut(s) 717
SspMI CTAG 5 cut(s) 93, 225, 270, 642, 1191
SstI GAGCTC 1 cut(s) 928
StyD4I CCNGG 5 cut(s) 178, 456, 652, 688, 1338
StyI CCWWGG 1 cut(s) 1097
TaaI ACNGT 3 cut(s) 14, 251, 1238
TaiI ACGT 5 cut(s) 621, 688, 755, 813, 1185
TaqI TCGA 3 cut(s) 888, 1132, 1322
TasI AATT 8 cut(s) 17, 137, 399, 844, 881, 1014, 1035, 1371
TatI WGTACW 2 cut(s) 1106, 1289
TauI GCSGC 3 cut(s) 76, 166, 283
TfiI GAWTC 8 cut(s) 56, 215, 326, 347, 425, 638, 740, 818
Tru1I TTAA 2 cut(s) 153, 407
Tru9I TTAA 2 cut(s) 153, 407
TscAI CASTG 2 cut(s) 551, 568
TseFI GTSAC 2 cut(s) 111, 1536
TseI GCWGC 6 cut(s) 221, 235, 829, 1216, 1361, 1364
Tsp45I GTSAC 2 cut(s) 111, 1536
TspDTI ATGAA 4 cut(s) 371, 603, 746, 1281
TspGWI ACGGA 2 cut(s) 1126, 1192
TspRI CASTG 2 cut(s) 551, 568
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 2 cut(s) 442, 704
VspI ATTAAT 1 cut(s) 153
XapI RAATTY 2 cut(s) 17, 844
XbaI TCTAGA 2 cut(s) 269, 641
XceI RCATGY 1 cut(s) 533
XhoI CTCGAG 2 cut(s) 887, 1321
XspI CTAG 5 cut(s) 93, 225, 270, 642, 1191
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.