Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009476

Physical Location & Seq

Genomic Coordinates Forward (+)

27537076 .. 27539961

Gene Structure 2886 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1193900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGAGCCTGCTCGGCTATCACCTAGCTGCTGGTCTGCGTTTTCAGATGGATGCACCCTCACATAGATTCTTGGAAGAGTTGCTTCAATCCGCTGTCGAAAATCATGAGGATGGTGGCGTTGTCAGTGAACAATCTGTGCAGCCTGCAACATGTGCTCTATGCCAAAGGGCTATTTTGCCCGAGAATGAGGCGACTGAAGATGTTGAAATTATGTGTGGGGACTGTAAATTTCTGTACCTTGAAGATCTTGGTACTCCCTCACATGATTCTTATCAGAGGACGCCGCCTACGAGAAGAAGAGCTAGGACCAGCAGTTCTGAGTCACTTGAGAATACTTTTTTGCAACAATTCTCGCATGTGATTAATCTGGTGCGGCAAAACCAATCCCCTGGCGCTGGGCTTGAGGAACTACCTGTGGATGGTGATTCTGCTGCTACGATACCGCGGCGCACAAGTAACCGTACTACACCAAGTGGGTCTAGAAGATGGCGGCGGGTACTCTCGGATACTGAAAGTGAGGGTTACGAAAATGTGGATTCTCTCTATGGGGAAACTGAATCAAATCTCAGTTTTGGTCGGTACAGGGTATTTCATGGTGAGAATGATGCAAATTCTTTTAGTGCGTATGGAGGGGACTCTGATGCTTCTGTGGACCTACATGGTTTCCTAGATATTGAAACAGTTATTCAACCAGATGAGGGAAGTGCTTATGATAGTGATACTGACATTGACCCAATGCATGCTGGTCTTAGCCAGTGGAACTCAGATGACGCTGAGGACGATGATGATGAGGGGGATGAAGAAGATGATGAATGGGAAGAAGCTGGTGTTGAAGTAAACATAGTTGAATCTAGGGAAACCCCAGGTGGTCGGCTTCGTAATCTTTTAGTTTCGAGTCCCGGCAGAAGTAATGGCCCATCCAATTGGCGCCAACAAATATATTCTCCTGAATCTGAGGGTATGATTCGTTATAGAATGAGGGGAACCAATCAAGTATATGACCTCTTTTCTAACGTGGAAGAATCAGAGTTGCTGCCTTATGTTGGAAATTCTGGTGATTATCTTGATGCTAGAGGCTTTGAAGAATTGCTCGAGCATCTTGCTGAGGTTGATAGCTTAAGACGAGGAGCACCTCCAGCTTCTGTTTCCCTTGTGAATAATCTGCCTCGTGTTGTCATTAGTAAGGAACATAACAAGCATGCTGACTTAGCCTGTGCAATTTGCAAGGATGTATTGCCAATTGGTACCGAAGTGAATCAGCTTCCCTGCTCTCACCTGTATCACTCTTCCTGTATTTTGCCATGGTTGAGTACACGGAATACTTGTCCACTCTGTCGATATGAGCTACCAACAGATGACAGAGACTACGAAGAGGGGAAACAGAACGTTGGTGCTAGAGCGGAGATCCACAACGTCCGGCAGCAGAATGCAAGTGAGGACAGTTCCTCCGGTGCTTTCAACAGAGCTGATGAAGATGAGGATGAAGAGCATGAGCAGAGTGCAGAAAATAGGGAACCAGCCACTGATGGCTCGAGAGGAGAAAATGGGCGGGGGAGATGGTTTTTTCTCGCTGCTGCTCCAATTGTCAGTCTCGTGGGGATTGTTCTTGTGCTGTGGTTAGGAAGTCCCCTGTTGGAAAGAAGACGACTGGCAGGAAACCAGAACTTTGCCAATCAGGCCCGCCACCAGAGTCATGTCACCGGCGCCTCACCCCACCACAGGGAGAACAGAAGCCGACCATGGTGGTCACTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2 zf-RING_2

Protein Analysis

586

Amino Acids

65.27

Weight (kDa)

4.66

Isoelectric Point (pI)

60.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	404 - 446	8.2e-12	Ring finger domain
zf-C3HC4_2	PF13923	405 - 445	2.6e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	405 - 445	3.7e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	405 - 432	5.5e-06	RING-like zinc finger
zf-rbx1	PF12678	406 - 446	1.7e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01980
fragaria_vesca	FvH4_6g09350 FvH4_6g09350 FvH4_6g09350
malus_domestica	MD04G1178400.v1.1 MD12G1193900.v1.1
prunus_persica	Prupe.6G298400_v2.0.a1
pyrus_communis	pycom04g15740 pycom12g18010
rosa_chinensis	RchiOBHm_Chr2g0091181 RchiOBHm_Chr3g0459741
rosa_laevigata	RLG00000016189 RLG00000025061
rosa_multiflora	Rmu_sc0000240.1_g000035
rosa_roxburghii	Rroxscaffold_2G00150420 Rroxscaffold_6G00420010
rosa_rugosa	Rorug02G0014100 Rorug03G0038100
rosa_samantha	Rh3AG096500 Rh3BG099200 Rh3CG101100 Rh3DG100500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1244
AccB1I	GGYRCC	3 cut(s)	927, 1244, 1703
AccBSI	CCGCTC	1 cut(s)	1400
AccII	CGCG	1 cut(s)	445
AclI	AACGTT	1 cut(s)	1386
AclWI	GGATC	1 cut(s)	1399
AcsI	RAATTY	3 cut(s)	227, 610, 1048
AcuI	CTGAAG	1 cut(s)	216
AcyI	GRCGYC	3 cut(s)	281, 928, 1704
AdeI	CACNNNGTG	1 cut(s)	473
AfaI	GTAC	7 cut(s)	236, 253, 463, 498, 581, 1246, 1312
AfiI	CCNNNNNNNGG	6 cut(s)	395, 419, 698, 1043, 1719, 1720
AflII	CTTAAG	1 cut(s)	1117
AflIII	ACRYGT	1 cut(s)	149
AgsI	TTSAA	9 cut(s)	86, 206, 242, 677, 689, 833, 848, 1082, 1459
AjnI	CCWGG	2 cut(s)	388, 862
AloI	GAACNNNNNNTCC	2 cut(s)	298, 330
AluBI	AGCT	8 cut(s)	26, 302, 824, 1116, 1139, 1261, 1345, 1466
AluI	AGCT	8 cut(s)	26, 302, 824, 1116, 1139, 1261, 1345, 1466
Alw21I	GWGCWC	2 cut(s)	157, 1132
Alw26I	GTCTC	2 cut(s)	1356, 1595
AlwI	GGATC	1 cut(s)	1399
AlwNI	CAGNNNCTG	2 cut(s)	1142, 1523
Ama87I	CYCGRG	3 cut(s)	179, 1091, 1531
AoxI	GGCC	2 cut(s)	913, 1677
ApeKI	GCWGC	7 cut(s)	26, 139, 431, 1033, 1420, 1571, 1574
ApoI	RAATTY	3 cut(s)	227, 610, 1048
AseI	ATTAAT	1 cut(s)	363
Asp718I	GGTACC	1 cut(s)	1244
AspLEI	GCGC	4 cut(s)	395, 450, 930, 1706
AspS9I	GGNCC	4 cut(s)	306, 652, 914, 1678
AsuC2I	CCSGG	1 cut(s)	900
AsuHPI	GGTGA	7 cut(s)	11, 434, 608, 1067, 1265, 1690, 1701
AvaI	CYCGRG	3 cut(s)	179, 1091, 1531
AvaII	GGWCC	2 cut(s)	306, 652
BaeI	ACNNNNGTAYC	2 cut(s)	1236, 1269
BanI	GGYRCC	3 cut(s)	927, 1244, 1703
BauI	CACGAG	2 cut(s)	1167, 1592
BbsI	GAAGAC	1 cut(s)	1648
Bbv12I	GWGCWC	2 cut(s)	157, 1132
BbvCI	CCTCAGC	2 cut(s)	774, 1104
BbvI	GCAGC	7 cut(s)	13, 151, 418, 1020, 1432, 1558, 1561
BccI	CCATC	7 cut(s)	40, 104, 413, 480, 925, 1520, 1551
BcgI	CGANNNNNNTGC	2 cut(s)	1082, 1116
BciT130I	CCWGG	2 cut(s)	390, 864
BciVI	GTATCC	1 cut(s)	499
BcnI	CCSGG	1 cut(s)	900
BcoDI	GTCTC	2 cut(s)	1356, 1595
BfaI	CTAG	7 cut(s)	23, 303, 480, 668, 852, 1071, 1395
BfoI	RGCGCY	3 cut(s)	396, 931, 1707
BfrI	CTTAAG	1 cut(s)	1117
BfuI	GTATCC	1 cut(s)	499
BglI	GCCNNNNNGGC	2 cut(s)	12, 1676
BglII	AGATCT	1 cut(s)	244
Bme1390I	CCNGG	3 cut(s)	390, 864, 900
Bme18I	GGWCC	2 cut(s)	306, 652
BmeT110I	CYCGRG	3 cut(s)	179, 1091, 1531
BmgT120I	GGNCC	4 cut(s)	306, 652, 914, 1678
BmiI	GGNNCC	5 cut(s)	929, 985, 1246, 1515, 1705
BmrFI	CCNGG	3 cut(s)	390, 864, 900
BmsI	GCATC	5 cut(s)	40, 595, 631, 1057, 1105
BpiI	GAAGAC	1 cut(s)	1648
BpmI	CTGGAG	1 cut(s)	1119
Bpu10I	CCTNAGC	2 cut(s)	774, 1104
BpuEI	CTTGAG	2 cut(s)	347, 422
BpuMI	CCSGG	1 cut(s)	900
BsaBI	GATNNNNATC	1 cut(s)	270
BsaHI	GRCGYC	3 cut(s)	281, 928, 1704
BsaJI	CCNNGG	5 cut(s)	388, 443, 862, 1301, 1739
BsaWI	WCCGGW	1 cut(s)	1448
BsaXI	ACNNNNNCTCC	2 cut(s)	1430, 1460
Bsc4I	CCNNNNNNNGG	6 cut(s)	395, 419, 698, 1043, 1719, 1720
Bse118I	RCCGGY	1 cut(s)	1700
Bse1I	ACTGG	2 cut(s)	754, 1653
Bse8I	GATNNNNATC	1 cut(s)	270
BseBI	CCWGG	2 cut(s)	390, 864
BseDI	CCNNGG	5 cut(s)	388, 443, 862, 1301, 1739
BseGI	GGATG	7 cut(s)	55, 115, 424, 802, 917, 1234, 1486
BseJI	GATNNNNATC	1 cut(s)	270
BseLI	CCNNNNNNNGG	6 cut(s)	395, 419, 698, 1043, 1719, 1720
BseMII	CTCAG	6 cut(s)	309, 580, 765, 777, 945, 1095
BseNI	ACTGG	2 cut(s)	754, 1653
BseRI	GAGGAG	2 cut(s)	1140, 1551
BseXI	GCAGC	7 cut(s)	13, 151, 418, 1020, 1432, 1558, 1561
BseYI	CCCAGC	1 cut(s)	395
BsgI	GTGCAG	2 cut(s)	158, 1521
Bsh1236I	CGCG	1 cut(s)	445
BshFI	GGCC	2 cut(s)	915, 1679
BshNI	GGYRCC	3 cut(s)	927, 1244, 1703
BsiHKAI	GWGCWC	2 cut(s)	157, 1132
BsiHKCI	CYCGRG	3 cut(s)	179, 1091, 1531
BsiSI	CCGG	4 cut(s)	900, 1417, 1449, 1701
BslFI	GGGAC	4 cut(s)	233, 647, 882, 1611
BslI	CCNNNNNNNGG	6 cut(s)	395, 419, 698, 1043, 1719, 1720
BsmAI	GTCTC	2 cut(s)	1356, 1595
BsmFI	GGGAC	4 cut(s)	233, 647, 882, 1611
BsmI	GAATGC	1 cut(s)	1432
BsnI	GGCC	2 cut(s)	915, 1679
BsoBI	CYCGRG	3 cut(s)	179, 1091, 1531
Bsp1286I	GDGCHC	2 cut(s)	157, 1132
Bsp143I	GATC	2 cut(s)	244, 1404
Bsp19I	CCATGG	2 cut(s)	1301, 1739
BspANI	GGCC	2 cut(s)	915, 1679
BspCNI	CTCAG	6 cut(s)	310, 579, 766, 776, 946, 1096
BspFNI	CGCG	1 cut(s)	445
BspHI	TCATGA	1 cut(s)	103
BspLI	GGNNCC	5 cut(s)	929, 985, 1246, 1515, 1705
BspPI	GGATC	1 cut(s)	1399
BspQI	GCTCTTC	2 cut(s)	292, 1479
BspT107I	GGYRCC	3 cut(s)	927, 1244, 1703
BspTI	CTTAAG	1 cut(s)	1117
BsrBI	CCGCTC	1 cut(s)	1400
BsrFI	RCCGGY	1 cut(s)	1700
BsrI	ACTGG	2 cut(s)	754, 1653
BssAI	RCCGGY	1 cut(s)	1700
BssECI	CCNNGG	5 cut(s)	388, 443, 862, 1301, 1739
BssMI	GATC	2 cut(s)	244, 1404
BssNI	GRCGYC	3 cut(s)	281, 928, 1704
BssSI	CACGAG	2 cut(s)	1167, 1592
BssT1I	CCWWGG	2 cut(s)	1301, 1739
Bst2BI	CACGAG	2 cut(s)	1167, 1592
Bst2UI	CCWGG	2 cut(s)	390, 864
Bst4CI	ACNGT	4 cut(s)	224, 461, 682, 1442
Bst6I	CTCTTC	5 cut(s)	69, 292, 1291, 1365, 1479
BstACI	GRCGYC	3 cut(s)	281, 928, 1704
BstAFI	CTTAAG	1 cut(s)	1117
BstAPI	GCANNNNNTGC	1 cut(s)	152
BstC8I	GCNNGC	5 cut(s)	8, 144, 741, 1200, 1681
BstDEI	CTNAG	8 cut(s)	318, 566, 749, 763, 774, 954, 1104, 1207
BstDSI	CCRYGG	3 cut(s)	443, 1301, 1739
BstF5I	GGATG	7 cut(s)	55, 115, 424, 802, 917, 1234, 1486
BstFNI	CGCG	1 cut(s)	445
BstH2I	RGCGCY	3 cut(s)	396, 931, 1707
BstHHI	GCGC	4 cut(s)	395, 450, 930, 1706
BstKTI	GATC	2 cut(s)	247, 1407
BstMAI	GTCTC	2 cut(s)	1356, 1595
BstMBI	GATC	2 cut(s)	244, 1404
BstMWI	GCNNNNNNNGC	5 cut(s)	12, 152, 1136, 1208, 1676
BstNI	CCWGG	2 cut(s)	390, 864
BstNSI	RCATGY	4 cut(s)	153, 359, 743, 1202
BstSCI	CCNGG	3 cut(s)	388, 862, 898
BstUI	CGCG	1 cut(s)	445
BstV1I	GCAGC	7 cut(s)	13, 151, 418, 1020, 1432, 1558, 1561
BstV2I	GAAGAC	1 cut(s)	1648
BstX2I	RGATCY	2 cut(s)	244, 1404
BstXI	CCANNNNNNTGG	2 cut(s)	389, 924
BstYI	RGATCY	2 cut(s)	244, 1404
BsuI	GTATCC	1 cut(s)	499
BsuRI	GGCC	2 cut(s)	915, 1679
BtgI	CCRYGG	3 cut(s)	443, 1301, 1739
BtsCI	GGATG	7 cut(s)	55, 115, 424, 802, 917, 1234, 1486
BtsIMutI	CAGTG	3 cut(s)	130, 761, 1521
Cac8I	GCNNGC	5 cut(s)	8, 144, 741, 1200, 1681
CaiI	CAGNNNCTG	2 cut(s)	1142, 1523
CciI	TCATGA	1 cut(s)	103
CfoI	GCGC	4 cut(s)	395, 450, 930, 1706
Cfr10I	RCCGGY	1 cut(s)	1700
Cfr13I	GGNCC	4 cut(s)	306, 652, 914, 1678
Cfr42I	CCGCGG	1 cut(s)	446
CseI	GACGC	2 cut(s)	289, 779
Csp6I	GTAC	7 cut(s)	235, 252, 462, 497, 580, 1245, 1311
CviQI	GTAC	7 cut(s)	235, 252, 462, 497, 580, 1245, 1311
DdeI	CTNAG	8 cut(s)	318, 566, 749, 763, 774, 954, 1104, 1207
DinI	GGCGCC	2 cut(s)	929, 1705
DpnI	GATC	2 cut(s)	246, 1406
DpnII	GATC	2 cut(s)	244, 1404
DraIII	CACNNNGTG	1 cut(s)	473
Eam1104I	CTCTTC	5 cut(s)	69, 292, 1291, 1365, 1479
EarI	CTCTTC	5 cut(s)	69, 292, 1291, 1365, 1479
Eco130I	CCWWGG	2 cut(s)	1301, 1739
Eco47I	GGWCC	2 cut(s)	306, 652
Eco57I	CTGAAG	1 cut(s)	216
Eco88I	CYCGRG	3 cut(s)	179, 1091, 1531
EcoRII	CCWGG	2 cut(s)	388, 862
EcoT14I	CCWWGG	2 cut(s)	1301, 1739
EcoT22I	ATGCAT	1 cut(s)	741
EgeI	GGCGCC	2 cut(s)	929, 1705
EheI	GGCGCC	2 cut(s)	929, 1705
ErhI	CCWWGG	2 cut(s)	1301, 1739
FalI	AAGNNNNNCTT	2 cut(s)	66, 98
FaqI	GGGAC	4 cut(s)	233, 647, 882, 1611
FauI	CCCGC	3 cut(s)	486, 1542, 1688
FokI	GGATG	7 cut(s)	62, 122, 431, 809, 904, 1241, 1493
FspBI	CTAG	7 cut(s)	23, 303, 480, 668, 852, 1071, 1395
GlaI	GCGC	4 cut(s)	394, 449, 929, 1705
GsaI	CCCAGC	1 cut(s)	399
GsuI	CTGGAG	1 cut(s)	1119
HaeII	RGCGCY	3 cut(s)	396, 931, 1707
HaeIII	GGCC	2 cut(s)	915, 1679
HapII	CCGG	4 cut(s)	900, 1417, 1449, 1701
HgaI	GACGC	2 cut(s)	289, 779
HhaI	GCGC	4 cut(s)	395, 450, 930, 1706
Hin1I	GRCGYC	3 cut(s)	281, 928, 1704
Hin6I	GCGC	4 cut(s)	393, 448, 928, 1704
HinP1I	GCGC	4 cut(s)	393, 448, 928, 1704
HpaII	CCGG	4 cut(s)	900, 1417, 1449, 1701
HphI	GGTGA	7 cut(s)	11, 434, 608, 1067, 1265, 1690, 1701
Hpy166II	GTNNAC	5 cut(s)	128, 652, 838, 1313, 1328
Hpy188I	TCNGA	9 cut(s)	45, 276, 319, 505, 640, 766, 955, 1027, 1757
Hpy188III	TCNNGA	5 cut(s)	104, 480, 947, 1064, 1533
Hpy8I	GTNNAC	5 cut(s)	128, 652, 838, 1313, 1328
HpyCH4III	ACNGT	4 cut(s)	224, 461, 682, 1442
HpyCH4IV	ACGT	3 cut(s)	1014, 1386, 1413
HpyF10VI	GCNNNNNNNGC	5 cut(s)	12, 152, 1136, 1208, 1676
HpyF3I	CTNAG	8 cut(s)	318, 566, 749, 763, 774, 954, 1104, 1207
HpySE526I	ACGT	3 cut(s)	1014, 1386, 1413
Hsp92I	GRCGYC	3 cut(s)	281, 928, 1704
HspAI	GCGC	4 cut(s)	393, 448, 928, 1704
KasI	GGCGCC	2 cut(s)	927, 1703
KpnI	GGTACC	1 cut(s)	1248
KspI	CCGCGG	1 cut(s)	446
Kzo9I	GATC	2 cut(s)	244, 1404
LguI	GCTCTTC	2 cut(s)	292, 1479
LmnI	GCTCC	2 cut(s)	1127, 1582
Lsp1109I	GCAGC	7 cut(s)	13, 151, 418, 1020, 1432, 1558, 1561
LweI	GCATC	5 cut(s)	40, 595, 631, 1057, 1105
MaeI	CTAG	7 cut(s)	23, 303, 480, 668, 852, 1071, 1395
MaeII	ACGT	3 cut(s)	1014, 1386, 1413
MaeIII	GTNAC	5 cut(s)	321, 455, 521, 1696, 1746
MalI	GATC	2 cut(s)	246, 1406
MbiI	CCGCTC	1 cut(s)	1400
MboI	GATC	2 cut(s)	244, 1404
MfeI	CAATTG	3 cut(s)	922, 1239, 1581
MflI	RGATCY	2 cut(s)	244, 1404
MhlI	GDGCHC	2 cut(s)	157, 1132
Mly113I	GGCGCC	2 cut(s)	928, 1704
MlyI	GAGTC	4 cut(s)	329, 629, 904, 1699
MmeI	TCCRAC	2 cut(s)	1024, 1614
Mph1103I	ATGCAT	1 cut(s)	741
MseI	TTAA	2 cut(s)	363, 1118
MslI	CAYNNNNRTG	1 cut(s)	108
MspA1I	CMGCKG	2 cut(s)	92, 445
MspCI	CTTAAG	1 cut(s)	1117
MspI	CCGG	4 cut(s)	900, 1417, 1449, 1701
MspR9I	CCNGG	3 cut(s)	390, 864, 900
MunI	CAATTG	3 cut(s)	922, 1239, 1581
Mva1269I	GAATGC	1 cut(s)	1432
MvaI	CCWGG	2 cut(s)	390, 864
MvnI	CGCG	1 cut(s)	445
MwoI	GCNNNNNNNGC	5 cut(s)	12, 152, 1136, 1208, 1676
NarI	GGCGCC	2 cut(s)	928, 1704
NciI	CCSGG	1 cut(s)	900
NcoI	CCATGG	2 cut(s)	1301, 1739
NdeII	GATC	2 cut(s)	244, 1404
NlaIV	GGNNCC	5 cut(s)	929, 985, 1246, 1515, 1705
NmuCI	GTSAC	3 cut(s)	321, 1696, 1746
NsiI	ATGCAT	1 cut(s)	741
NspI	RCATGY	4 cut(s)	153, 359, 743, 1202
PaeI	GCATGC	2 cut(s)	743, 1202
PaeR7I	CTCGAG	2 cut(s)	1091, 1531
PagI	TCATGA	1 cut(s)	103
PciI	ACATGT	1 cut(s)	149
PciSI	GCTCTTC	2 cut(s)	292, 1479
PcsI	WCGNNNNNNNCGW	2 cut(s)	287, 777
PctI	GAATGC	1 cut(s)	1432
PleI	GAGTC	4 cut(s)	328, 629, 903, 1698
PluTI	GGCGCC	2 cut(s)	931, 1707
PpsI	GAGTC	4 cut(s)	328, 629, 903, 1698
PscI	ACATGT	1 cut(s)	149
PshBI	ATTAAT	1 cut(s)	363
Psp1406I	AACGTT	1 cut(s)	1386
Psp6I	CCWGG	2 cut(s)	388, 862
PspFI	CCCAGC	1 cut(s)	395
PspGI	CCWGG	2 cut(s)	388, 862
PspN4I	GGNNCC	5 cut(s)	929, 985, 1246, 1515, 1705
PspPI	GGNCC	4 cut(s)	306, 652, 914, 1678
PspXI	VCTCGAGB	1 cut(s)	1091
PstNI	CAGNNNCTG	2 cut(s)	1142, 1523
PsuI	RGATCY	2 cut(s)	244, 1404
RsaI	GTAC	7 cut(s)	236, 253, 463, 498, 581, 1246, 1312
RsaNI	GTAC	7 cut(s)	235, 252, 462, 497, 580, 1245, 1311
RseI	CAYNNNNRTG	1 cut(s)	108
SacII	CCGCGG	1 cut(s)	446
SapI	GCTCTTC	2 cut(s)	292, 1479
SaqAI	TTAA	2 cut(s)	363, 1118
Sau3AI	GATC	2 cut(s)	244, 1404
Sau96I	GGNCC	4 cut(s)	306, 652, 914, 1678
SchI	GAGTC	4 cut(s)	329, 629, 904, 1699
ScrFI	CCNGG	3 cut(s)	390, 864, 900
SduI	GDGCHC	2 cut(s)	157, 1132
SfaNI	GCATC	5 cut(s)	40, 595, 631, 1057, 1105
SfoI	GGCGCC	2 cut(s)	929, 1705
Sfr274I	CTCGAG	2 cut(s)	1091, 1531
Sfr303I	CCGCGG	1 cut(s)	446
SgrAI	CRCCGGYG	1 cut(s)	1700
SgrBI	CCGCGG	1 cut(s)	446
SinI	GGWCC	2 cut(s)	306, 652
SlaI	CTCGAG	2 cut(s)	1091, 1531
SmiMI	CAYNNNNRTG	1 cut(s)	108
SmlI	CTYRAG	5 cut(s)	326, 401, 1091, 1117, 1531
SmoI	CTYRAG	5 cut(s)	326, 401, 1091, 1117, 1531
SphI	GCATGC	2 cut(s)	743, 1202
SspDI	GGCGCC	2 cut(s)	927, 1703
SspMI	CTAG	7 cut(s)	23, 303, 480, 668, 852, 1071, 1395
StyD4I	CCNGG	3 cut(s)	388, 862, 898
StyI	CCWWGG	2 cut(s)	1301, 1739
TaaI	ACNGT	4 cut(s)	224, 461, 682, 1442
TaiI	ACGT	3 cut(s)	1017, 1389, 1416
TaqI	TCGA	5 cut(s)	96, 893, 1092, 1336, 1532
TatI	WGTACW	1 cut(s)	1310
TauI	GCSGC	4 cut(s)	286, 376, 448, 493
Tru1I	TTAA	2 cut(s)	363, 1118
Tru9I	TTAA	2 cut(s)	363, 1118
TscAI	CASTG	3 cut(s)	130, 761, 1528
TseFI	GTSAC	3 cut(s)	321, 1696, 1746
TseI	GCWGC	7 cut(s)	26, 139, 431, 1033, 1420, 1571, 1574
Tsp45I	GTSAC	3 cut(s)	321, 1696, 1746
TspDTI	ATGAA	5 cut(s)	581, 813, 825, 1485, 1497
TspGWI	ACGGA	1 cut(s)	1330
TspRI	CASTG	3 cut(s)	130, 761, 1528
Vha464I	CTTAAG	1 cut(s)	1117
VpaK11BI	GGWCC	2 cut(s)	306, 652
VspI	ATTAAT	1 cut(s)	363
XapI	RAATTY	3 cut(s)	227, 610, 1048
XbaI	TCTAGA	1 cut(s)	479
XceI	RCATGY	4 cut(s)	153, 359, 743, 1202
XhoI	CTCGAG	2 cut(s)	1091, 1531
XspI	CTAG	7 cut(s)	23, 303, 480, 668, 852, 1071, 1395
Zsp2I	ATGCAT	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.