MD04G1180000.v1.1

Cell division control protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016409
Physical Location & Seq
Reverse (-)
27097204 .. 27098888
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1180000.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGGGCGTGGCGTGCGCACGTCGCGCTCCTCCAGATGCTCCATTCTCCGCTGCAGTCGCGAAAGGCCACCGCATTGTCGATTGCTCGAAGCGTCGAAAGCGAAGAAGGGGGAACCCGTCTACTTGGTGTTCGAGTATTTGGACACCGATCTCAAGAAGTTCATCGATTCACACAGAAAGGGGCCGAATCCTGGGCCGATGCCACCGTCGCTGGTGCAGAGCTTTATGTACCAGCTGTGCAAGGGGGTTGCGCACTGCCACTCCCACGGCGTCCTCCACCGCGATCTGAAGCCGCAGAATCTGCTTCTTGACAAGGAAAGGGGGATTCTGAAGATCGCTGATCTCGGACTCGGCCGCGCATTCACTGTGCCTCTCAAGAGCTACACGCACGAAATTGTTACTCTCTGGTACAGAGCGCCCGAGGTTCTGCTCGGTTCGGCTCACTACTCCACCGGCGTTGATATGTGGTCCGTAGGATGCATCTTCGCGGAGATGGCGAGGAGGCAGGCTCTTTTTCCGGGAGATTCTGAGTTCCAGCAGTTGCTTCACATTTTCAGGCTGCTAGGAACACCATCTGACAAGCAGTGGCCAGGAGTTTCTTCTCTGCGAGATTGGCACGTGTATCCGCAGTGGGAGCCTCAGAACTTGGCTCGCGCTGTTCCTGCTTTGGAGCCTGAAGGAGTTGACCTCCTAGCCAAAATGCTTAAATACGATCCAGCTGAGCGAATCTCCGCGAAAGCTGCCCTTGACCACCCCTATTTCGACACCCTCGACAAGTCTCAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

263

Amino Acids

29.72

Weight (kDa)

9.08

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 36 - 254 2.6e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 36 - 168 5.5e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 17, 252
AccI GTMKAC 1 cut(s) 120
AccII CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
AciI CCGC 8 cut(s) 49, 71, 280, 293, 355, 488, 626, 732
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 2 cut(s) 352, 587
AcuI CTGAAG 3 cut(s) 308, 350, 696
AcvI CACGTG 1 cut(s) 619
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 2 cut(s) 230, 410
AfiI CCNNNNNNNGG 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 618
AjiI CACGTC 1 cut(s) 21
AjnI CCWGG 2 cut(s) 190, 589
AluBI AGCT 5 cut(s) 222, 235, 381, 719, 740
AluI AGCT 5 cut(s) 222, 235, 381, 719, 740
Alw26I GTCTC 1 cut(s) 783
AlwI GGATC 1 cut(s) 707
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 419
AoxI GGCC 5 cut(s) 65, 182, 194, 352, 587
ApeKI GCWGC 3 cut(s) 51, 559, 740
AspLEI GCGC 6 cut(s) 18, 27, 253, 359, 418, 656
AspS9I GGNCC 3 cut(s) 182, 194, 468
AsuC2I CCSGG 1 cut(s) 519
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 589
BbrPI CACGTG 1 cut(s) 619
BbvI GCAGC 3 cut(s) 38, 546, 727
BccI CCATC 2 cut(s) 487, 580
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 192, 591
BciVI GTATCC 1 cut(s) 633
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 783
BfaI CTAG 2 cut(s) 563, 692
BfmI CTRYAG 1 cut(s) 52
BfoI RGCGCY 1 cut(s) 419
BfuI GTATCC 1 cut(s) 633
BisI GCNGC 5 cut(s) 52, 293, 355, 560, 741
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 5 cut(s) 53, 294, 356, 561, 742
Bme1390I CCNGG 3 cut(s) 192, 519, 591
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 419
BmgBI CACGTC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 182, 194, 468
BmiI GGNNCC 4 cut(s) 114, 183, 636, 672
BmrFI CCNGG 3 cut(s) 192, 519, 591
BmsI GCATC 4 cut(s) 26, 189, 467, 489
BplI GAGNNNNNCTC 6 cut(s) 493, 525, 672, 704, 713, 745
BpmI CTGGAG 1 cut(s) 16
Bpu1102I GCTNAGC 1 cut(s) 720
BpuEI CTTGAG 2 cut(s) 137, 359
BpuMI CCSGG 1 cut(s) 519
Bsa29I ATCGAT 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 619
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 191, 265, 420
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse118I RCCGGY 1 cut(s) 452
BseBI CCWGG 2 cut(s) 192, 591
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 191, 265, 420
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMII CTCAG 3 cut(s) 519, 653, 711
BseRI GAGGAG 2 cut(s) 19, 514
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 3 cut(s) 38, 546, 727
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 5 cut(s) 67, 184, 196, 354, 589
BshVI ATCGAT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 355
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 2 cut(s) 453, 518
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 359
BsnI GGCC 5 cut(s) 67, 184, 196, 354, 589
BsoBI CYCGRG 1 cut(s) 419
Bsp143I GATC 5 cut(s) 148, 283, 333, 340, 712
Bsp1720I GCTNAGC 1 cut(s) 720
Bsp68I TCGCGA 1 cut(s) 60
BspACI CCGC 8 cut(s) 49, 71, 280, 293, 355, 488, 626, 732
BspANI GGCC 5 cut(s) 67, 184, 196, 354, 589
BspCNI CTCAG 3 cut(s) 520, 652, 712
BspDI ATCGAT 1 cut(s) 165
BspFNI CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
BspLI GGNNCC 4 cut(s) 114, 183, 636, 672
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 1 cut(s) 707
BsrFI RCCGGY 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 3 cut(s) 191, 265, 420
BssMI GATC 5 cut(s) 148, 283, 333, 340, 712
BssNI GRCGYC 1 cut(s) 270
Bst2UI CCWGG 2 cut(s) 192, 591
Bst4CI ACNGT 2 cut(s) 207, 367
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 301
BstBAI YACGTR 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 14, 507, 652
BstDEI CTNAG 4 cut(s) 528, 639, 720, 780
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 482
BstFNI CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 6 cut(s) 18, 27, 253, 359, 418, 656
BstKTI GATC 5 cut(s) 151, 286, 336, 343, 715
BstMAI GTCTC 1 cut(s) 783
BstMBI GATC 5 cut(s) 148, 283, 333, 340, 712
BstMCI CGRYCG 1 cut(s) 355
BstNI CCWGG 2 cut(s) 192, 591
BstSCI CCNGG 3 cut(s) 190, 517, 589
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
BstV1I GCAGC 3 cut(s) 38, 546, 727
BstZI CGGCCG 1 cut(s) 352
Bsu15I ATCGAT 1 cut(s) 165
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 5 cut(s) 67, 184, 196, 354, 589
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 21
BtsCI GGATG 1 cut(s) 482
BtsI GCAGTG 3 cut(s) 253, 590, 635
BtsIMutI CAGTG 4 cut(s) 253, 363, 590, 635
BtuMI TCGCGA 1 cut(s) 60
Cac8I GCNNGC 3 cut(s) 14, 507, 652
CaiI CAGNNNCTG 1 cut(s) 301
CfoI GCGC 6 cut(s) 18, 27, 253, 359, 418, 656
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 182, 194, 468
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 2 cut(s) 81, 259
Csp6I GTAC 2 cut(s) 229, 409
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 2 cut(s) 229, 409
DdeI CTNAG 4 cut(s) 528, 639, 720, 780
DpnI GATC 5 cut(s) 150, 285, 335, 342, 714
DpnII GATC 5 cut(s) 148, 283, 333, 340, 712
EaeI YGGCCR 2 cut(s) 352, 587
EagI CGGCCG 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 468
Eco52I CGGCCG 1 cut(s) 352
Eco57I CTGAAG 3 cut(s) 308, 350, 696
Eco72I CACGTG 1 cut(s) 619
Eco88I CYCGRG 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 190, 589
EcoT22I ATGCAT 1 cut(s) 482
FaiI YATR 2 cut(s) 227, 464
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 52, 293, 355, 560, 741
FokI GGATG 1 cut(s) 489
Fsp4HI GCNGC 5 cut(s) 52, 293, 355, 560, 741
FspAI RTGCGCAY 1 cut(s) 17
FspBI CTAG 2 cut(s) 563, 692
FspI TGCGCA 2 cut(s) 17, 252
GlaI GCGC 6 cut(s) 17, 26, 252, 358, 417, 655
GluI GCNGC 5 cut(s) 52, 293, 355, 560, 741
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 419
HaeIII GGCC 5 cut(s) 67, 184, 196, 354, 589
HapII CCGG 2 cut(s) 453, 518
HgaI GACGC 2 cut(s) 81, 259
HhaI GCGC 6 cut(s) 18, 27, 253, 359, 418, 656
Hin1I GRCGYC 1 cut(s) 270
Hin6I GCGC 6 cut(s) 16, 25, 251, 357, 416, 654
HinP1I GCGC 6 cut(s) 16, 25, 251, 357, 416, 654
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 7 cut(s) 167, 187, 298, 325, 348, 524, 726
HpaII CCGG 2 cut(s) 453, 518
Hpy166II GTNNAC 2 cut(s) 121, 685
Hpy188I TCNGA 7 cut(s) 288, 330, 347, 529, 577, 642, 788
Hpy188III TCNNGA 5 cut(s) 33, 59, 154, 308, 376
Hpy8I GTNNAC 2 cut(s) 121, 685
Hpy99I CGWCG 3 cut(s) 25, 97, 211
HpyAV CCTTC 2 cut(s) 100, 671
HpyCH4III ACNGT 2 cut(s) 207, 367
HpyCH4IV ACGT 2 cut(s) 20, 618
HpyCH4V TGCA 4 cut(s) 54, 217, 240, 480
HpyF3I CTNAG 4 cut(s) 528, 639, 720, 780
HpySE526I ACGT 2 cut(s) 20, 618
Hsp92I GRCGYC 1 cut(s) 270
HspAI GCGC 6 cut(s) 16, 25, 251, 357, 416, 654
Kzo9I GATC 5 cut(s) 148, 283, 333, 340, 712
LmnI GCTCC 4 cut(s) 32, 44, 634, 670
Lsp1109I GCAGC 3 cut(s) 38, 546, 727
LweI GCATC 4 cut(s) 26, 189, 467, 489
MaeI CTAG 2 cut(s) 563, 692
MaeII ACGT 2 cut(s) 20, 618
MaeIII GTNAC 1 cut(s) 397
MalI GATC 5 cut(s) 150, 285, 335, 342, 714
MboI GATC 5 cut(s) 148, 283, 333, 340, 712
MboII GAAGA 4 cut(s) 115, 343, 475, 591
MlsI TGGCCA 1 cut(s) 589
MluCI AATT 1 cut(s) 393
MluNI TGGCCA 1 cut(s) 589
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 9 cut(s) 40, 284, 381, 415, 492, 495, 648, 698, 779
Mox20I TGGCCA 1 cut(s) 589
Mph1103I ATGCAT 1 cut(s) 482
MscI TGGCCA 1 cut(s) 589
MseI TTAA 1 cut(s) 705
Msp20I TGGCCA 1 cut(s) 589
MspA1I CMGCKG 3 cut(s) 51, 235, 719
MspI CCGG 2 cut(s) 453, 518
MspR9I CCNGG 3 cut(s) 192, 519, 591
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 2 cut(s) 192, 591
MvnI CGCG 7 cut(s) 25, 60, 282, 357, 488, 654, 734
NciI CCSGG 1 cut(s) 519
NdeII GATC 5 cut(s) 148, 283, 333, 340, 712
NlaIV GGNNCC 4 cut(s) 114, 183, 636, 672
NmeAIII GCCGAG 1 cut(s) 330
NruI TCGCGA 1 cut(s) 60
NsbI TGCGCA 2 cut(s) 17, 252
NsiI ATGCAT 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 768
PctI GAATGC 1 cut(s) 359
PfeI GAWTC 6 cut(s) 167, 187, 298, 325, 524, 726
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 5 cut(s) 53, 294, 356, 561, 742
PleI GAGTC 1 cut(s) 342
PmaCI CACGTG 1 cut(s) 619
PmlI CACGTG 1 cut(s) 619
PpsI GAGTC 1 cut(s) 342
Ppu21I YACGTR 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 190, 589
PspCI CACGTG 1 cut(s) 619
PspGI CCWGG 2 cut(s) 190, 589
PspN4I GGNNCC 4 cut(s) 114, 183, 636, 672
PspPI GGNCC 3 cut(s) 182, 194, 468
PstI CTGCAG 1 cut(s) 56
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 235, 719
RruI TCGCGA 1 cut(s) 60
RsaI GTAC 2 cut(s) 230, 410
RsaNI GTAC 2 cut(s) 229, 409
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 5 cut(s) 52, 293, 355, 560, 741
Sau3AI GATC 5 cut(s) 148, 283, 333, 340, 712
Sau96I GGNCC 3 cut(s) 182, 194, 468
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 3 cut(s) 192, 519, 591
SetI ASST 9 cut(s) 23, 224, 237, 383, 426, 621, 690, 721, 742
SfaNI GCATC 4 cut(s) 26, 189, 467, 489
SfcI CTRYAG 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 452
SinI GGWCC 1 cut(s) 468
SmlI CTYRAG 2 cut(s) 152, 374
SmoI CTYRAG 2 cut(s) 152, 374
Sse9I AATT 1 cut(s) 393
SsiI CCGC 8 cut(s) 49, 71, 280, 293, 355, 488, 626, 732
SspMI CTAG 2 cut(s) 563, 692
StyD4I CCNGG 3 cut(s) 190, 517, 589
TaaI ACNGT 2 cut(s) 207, 367
TaiI ACGT 2 cut(s) 23, 621
TaqI TCGA 7 cut(s) 79, 87, 95, 132, 165, 762, 771
TasI AATT 1 cut(s) 393
TauI GCSGC 2 cut(s) 295, 357
TfiI GAWTC 6 cut(s) 167, 187, 298, 325, 524, 726
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 4 cut(s) 260, 370, 590, 635
TseI GCWGC 3 cut(s) 51, 559, 740
TspDTI ATGAA 1 cut(s) 151
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 4 cut(s) 260, 370, 590, 635
VpaK11BI GGWCC 1 cut(s) 468
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 2 cut(s) 563, 692
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.