RLG00000025082

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016409
Physical Location & Seq
Reverse (-)
41836448 .. 41836795
348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025082

Sequence Viewer

Length: 348 bp
ATGGAGCTCGATCGATTCAAATTCAATCGAGTCTATAAGAAGGGGTTTGAGGCTCTCGATGGTGAGACTAGAGACGTTGTATTCGTCAAGAGAGTCGTGAGGTCGAGTTTGAGGCAAGAAGAAGCACATTTGACCCATGCTGAGGCCTCCAGTGGTGCCACCATACTCAGAACTTTGCCGGAGCACATCAATGTGATATCCTACGTCGCGAGTGGCGAGGCAAGTCCTGATTTCTATTGGGTTGTGGATTTCTTCGACGAGGATCTGCGCAGTTTGATCAACGAACCTGGTGGTCACCACGTTAACATGCCGCGTATTCGCTTCATCATGCGTCGAATAGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

13.33

Weight (kDa)

6.51

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 155
AccII CGCG 2 cut(s) 209, 313
AciI CCGC 1 cut(s) 311
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 1 cut(s) 20
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 2 cut(s) 19, 25
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 1 cut(s) 7
AluI AGCT 1 cut(s) 7
Alw21I GWGCWC 2 cut(s) 9, 186
Alw26I GTCTC 2 cut(s) 59, 66
AlwI GGATC 1 cut(s) 270
AoxI GGCC 1 cut(s) 144
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 74, 287
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 9, 186
BbvCI CCTCAGC 1 cut(s) 141
BccI CCATC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 288
BclI TGATCA 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 59, 66
BfaI CTAG 1 cut(s) 69
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
Bme1390I CCNGG 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 13
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 288
BseCI ATCGAT 1 cut(s) 13
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 132, 181
BseNI ACTGG 1 cut(s) 150
Bsh1236I CGCG 2 cut(s) 209, 313
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 13
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 9, 186
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 59, 66
BsmBI CGTCTC 1 cut(s) 66
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 9, 186
Bsp143I GATC 3 cut(s) 10, 262, 276
Bsp68I TCGCGA 1 cut(s) 209
BspACI CCGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 133, 180
BspDI ATCGAT 1 cut(s) 13
BspFNI CGCG 2 cut(s) 209, 313
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 150
BssMI GATC 3 cut(s) 10, 262, 276
Bst2UI CCWGG 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 141, 167
BstEII GGTNACC 1 cut(s) 293
BstFNI CGCG 2 cut(s) 209, 313
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 3 cut(s) 13, 265, 279
BstMAI GTCTC 2 cut(s) 59, 66
BstMBI GATC 3 cut(s) 10, 262, 276
BstMCI CGRYCG 1 cut(s) 13
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 310
BstPI GGTNACC 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 286
BstUI CGCG 2 cut(s) 209, 313
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 1 cut(s) 146
BsuTUI ATCGAT 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 157
BtuMI TCGCGA 1 cut(s) 209
CfoI GCGC 1 cut(s) 270
ClaI ATCGAT 1 cut(s) 13
CseI GACGC 1 cut(s) 320
CsiI ACCWGGT 1 cut(s) 286
CviAII CATG 3 cut(s) 137, 307, 328
CviJI RGCY 3 cut(s) 7, 53, 146
CviKI_1 RGCY 3 cut(s) 7, 53, 146
DdeI CTNAG 2 cut(s) 141, 167
DpnI GATC 3 cut(s) 12, 264, 278
DpnII GATC 3 cut(s) 10, 262, 276
Ecl136II GAGCTC 1 cut(s) 7
Eco147I AGGCCT 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 7
Eco91I GGTNACC 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 286
EcoRV GATATC 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 3 cut(s) 140, 310, 331
FaiI YATR 6 cut(s) 36, 138, 164, 308, 329, 346
FatI CATG 3 cut(s) 136, 306, 327
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 1 cut(s) 69
FspI TGCGCA 1 cut(s) 269
GlaI GCGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 270
Hin1II CATG 3 cut(s) 140, 310, 331
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 15, 30, 93
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 1 cut(s) 179
HphI GGTGA 2 cut(s) 74, 287
Hpy166II GTNNAC 1 cut(s) 304
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 5 cut(s) 56, 88, 97, 208, 227
Hpy8I GTNNAC 1 cut(s) 304
Hpy99I CGWCG 3 cut(s) 209, 260, 336
HpyAV CCTTC 1 cut(s) 34
HpyCH4IV ACGT 3 cut(s) 75, 204, 300
HpyF3I CTNAG 2 cut(s) 141, 167
HpySE526I ACGT 3 cut(s) 75, 204, 300
Hsp92II CATG 3 cut(s) 140, 310, 331
HspAI GCGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 3 cut(s) 10, 262, 276
LmnI GCTCC 2 cut(s) 4, 181
LpnPI CCDG 5 cut(s) 163, 192, 240, 273, 300
MabI ACCWGGT 1 cut(s) 286
MaeI CTAG 1 cut(s) 69
MaeII ACGT 3 cut(s) 75, 204, 300
MaeIII GTNAC 1 cut(s) 293
MalI GATC 3 cut(s) 12, 264, 278
MboI GATC 3 cut(s) 10, 262, 276
MboII GAAGA 2 cut(s) 131, 244
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 9, 186
MluCI AATT 1 cut(s) 20
MlyI GAGTC 2 cut(s) 39, 102
MnlI CCTC 7 cut(s) 43, 93, 105, 136, 157, 211, 253
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 2 cut(s) 189, 191
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 2 cut(s) 209, 313
NdeII GATC 3 cut(s) 10, 262, 276
NlaIII CATG 3 cut(s) 140, 310, 331
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 293
NruI TCGCGA 1 cut(s) 209
NsbI TGCGCA 1 cut(s) 269
NspI RCATGY 1 cut(s) 310
PceI AGGCCT 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 213
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 312
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 2 cut(s) 38, 101
PpsI GAGTC 2 cut(s) 38, 101
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 286
PspEI GGTNACC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 262
PvuI CGATCG 1 cut(s) 13
RruI TCGCGA 1 cut(s) 209
RseI CAYNNNNRTG 2 cut(s) 189, 191
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 10, 262, 276
SchI GAGTC 2 cut(s) 39, 102
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 2 cut(s) 9, 186
SetI ASST 6 cut(s) 9, 78, 104, 207, 289, 303
SexAI ACCWGGT 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 189, 191
Sse9I AATT 1 cut(s) 20
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 1 cut(s) 311
SspMI CTAG 1 cut(s) 69
SstI GAGCTC 1 cut(s) 9
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 286
TaiI ACGT 3 cut(s) 78, 207, 303
TaqI TCGA 7 cut(s) 9, 13, 28, 57, 104, 255, 334
TasI AATT 1 cut(s) 20
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 313
TspRI CASTG 1 cut(s) 157
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 310
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.