Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003096

Physical Location & Seq

Genomic Coordinates Reverse (-)

27273902 .. 27278398

Gene Structure 4497 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1181800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGGTTCGAGGGGGATTATCAGGAATGAACGTGAAAGGGGAAATTGAAAGTGACATTGCGGGGCTCTCTGAGTTAAGATCCTTGGATCTTTCATTCAACAAAGGCCTCACCGGTTCTCTCTCTCCACGATTAGGGGATCTGAGCAATCTAAACATCCTGATCCTAGCTGGTTGCAGCTTCAGTGGAATTATTCCAAGTGAATTGGGGAAACTTGGAAAGCTAACCTTCTTGGCTTTGAATACGAATAACTTCACCGGTAGTATACCTGCTTCTTTGGGTAATCTCTCCAACCTCTATTGGCTGGACCTGGCAGACAATCAGTTGACTGGACCTCTCCCAGTTTCGAGCGGCACTACCCCCGGCTTAGACCAACTGTTTCAGGCTAAACATTTCCATTTGAACCAGAACCAGCTTTCAGGTACCATTCCACCCAGACTTTTCAGCTCCAAGATGACACTGATCCATGTATTGTTTGATGGAAATAGATTCACTGGGACTATTCCATCAACAATATTTTCCGTTCAGACTCTCGAGGTTCTTCGGCTTGATAGAAATGCTCTGACGGGATCAGTCTCCTCAAATATCTCCAACCTAATAAATCTCAGTAACTTAAATATCGCGCACAACAATTTGGACGGCCCTTTACCCGACCTAACTGGATTGAATGCCCTCAATTATGTAGACCTTAGTTACAACTCATTTGATCCATCAGAAGCTCCGGGTTGGATCTCAACCTTGCCATCGCTCACCACTATCGTTTTAGAAGATGTGGCTCTTCAAGGGACTGTGCCAGAGAAGATGTTCAGCATACCGTCATTGCAGCAAGTACTTGTTGGGAACCCATTGTGCAATGAACCTAGCTCGAGTCCGTACTGTCAGCCTCCACAATCAAATACAAAACCGTATGCTACTAGCACGGATTGTCCATCCATTACATGCCCCGGGGGTCAGCAGCCAAGCCCTCAGAGTTGTCAATGTGGATATCCTTTTGAAGGAACACTGGATTTCCGAGCGCCCACTTTCAGGGAATTGACAAATGTGACTCTGTTTCAATCCCTAGAACAATGGGTGAAACTTCACCTCACTCCCGGTTCAGTGTCACTTGATAACCCTTTCTTCGACAACGATGACCATCTTAAAGTACATGTGGCTCTCTTTCCACCTACAGGAACATATTTTAATAGGTCTGAGATTATCAGGATTGGTTTTGATATGAGCAGTCAGCCTTACATGCCGCCAGACGGGTTTGGACCCTTCGTTTTTATTCCAGCTCAATATAATTTCCCAGTTAGTTGTCTCGTTCTTGTTGTGGGCCTCGTGGTAGTAGGAATTTACGCCATTAGGCAAAGGAAACGTGCGGAAAGAGCCATTGGTTTAAGCAGACCTTTCGCTTCTTGGGTGCCAAGCGGAAATGATAGTGGCGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCGTATGATGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAGTAATGAGATAGGATCTGGCGGCTACGGGAAGGTTTACAGGGGCATGCTTTCTGATGGACAAGTGATAGCAATCAAAAGAGCTCGGCAAGGATCAATGCAGGGAGGCGTCGAGTTCACGACTGAAATTGAGTTGCTCTCTCGCGTTCATCACAAAAATGTTGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTATATGCCTAATGGAACACTTAGGGAAAGCTTGTCAGGGAGATCTGGTATTCATCTCAATTGGAAGAGGAGACTCCAAATCACTCTTGGCTCAGCAAGAGGACTGGCTTACCTACATGAGCTTGCAAATCCTCCTATAATTCACAGAGATGTGAAGTCCACCAATATTCTATTAGATGAACATTTAACGGCGAAGGTTACAGATTTTGGCCTGTCGAAGCTGGTCAATGACAGCGGAAAGGGACATGTCTCGACTCAGGTCAAAGGAACACTAGGCTATATGGATCCTGAGTACTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCAGCCAATCGAGAAGGGAAAATACATTGTCCGTGAGGTACAATTGGTGATGGACAAGAATGACGAGGAGCATTACGGTTTGAGGGAGTTAACAGATCGAAACATTCGAAACTCAGGAACTCTTATTGGGTTTGGGAGGTTCTTGGAGCTAGCCATGCAATGCGTCGAAGAATCAGCTGCAGATCGTCCCACGATGAGTGAACTTGTGAAGGCTATCGAAACCATTCTGCAGAACGATGGGATGAACACAAACTCAACATCAGCATCTTCATCAGCCACGGAGTTTGCAGCATCAAAAGGTGCTCCGAAACATCCCTACAACGTTGGCTTGCCGAAAAAGGAATTCAATGACAGCACCGGCTCGTTTGATTACAGCGGTGGATGCGCTGTTTCAGCGAAAATTGAACCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 Pkinase_Tyr

Protein Analysis

833

Amino Acids

90.76

Weight (kDa)

5.87

Isoelectric Point (pI)

45.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	498 - 765	1.4e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	501 - 766	4.4e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003096)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79620 AT1G79620 AT1G79620 AT1G79620 AT1G79620
fragaria_vesca	FvH4_6g09020 FvH4_6g09020
malus_domestica	MD04G1181800.v1.1 MD04G1182200.v1.1 MD04G1183000.v1.1 MD04G1183100.v1.1 MD12G1196600.v1.1
prunus_persica	Prupe.6G301100_v2.0.a1 Prupe.6G301100_v2.0.a1 Prupe.6G301200_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1
pyrus_communis	pycom04g16070 pycom04g16150 pycom04g16170 pycom12g18280
rosa_chinensis	RchiOBHm_Chr3g0459271
rosa_laevigata	RLG00000025098
rosa_multiflora	Rmu_sc0006220.1_g000001 Rmu_sc0010702.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420350
rosa_rugosa	Rorug03G0034900 Rorug03G0035000
rosa_samantha	Rh3AG092700 Rh3BG095800 Rh3CG096800 Rh3DG096400
rosa_wichuraiana	Rw3G007970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	274
Acc65I	GGTACC	1 cut(s)	419
AccB1I	GGYRCC	2 cut(s)	419, 1399
AccBSI	CCGCTC	1 cut(s)	348
AccI	GTMKAC	3 cut(s)	262, 681, 2043
AccII	CGCG	2 cut(s)	620, 1641
AciI	CCGC	9 cut(s)	59, 348, 1235, 1358, 1407, 1422, 1518, 1947, 2460
AclI	AACGTT	1 cut(s)	2406
AcsI	RAATTY	2 cut(s)	1329, 2426
AcuI	CTGAAG	1 cut(s)	163
AcyI	GRCGYC	1 cut(s)	1605
AfaI	GTAC	6 cut(s)	421, 828, 872, 1143, 2006, 2124
AfiI	CCNNNNNNNGG	5 cut(s)	131, 992, 1023, 1166, 1241
AflII	CTTAAG	1 cut(s)	1469
AflIII	ACRYGT	2 cut(s)	1144, 1957
AgeI	ACCGGT	2 cut(s)	110, 254
AjnI	CCWGG	1 cut(s)	306
AjuI	GAANNNNNNNTTGG	2 cut(s)	1353, 1385
Alw21I	GWGCWC	4 cut(s)	1429, 1481, 1582, 2389
Alw26I	GTCTC	4 cut(s)	577, 1301, 1777, 1966
Alw44I	GTGCAC	2 cut(s)	1425, 1477
AlwNI	CAGNNNCTG	1 cut(s)	1702
Ama87I	CYCGRG	3 cut(s)	530, 862, 941
AoxI	GGCC	4 cut(s)	103, 637, 1312, 1921
ApaLI	GTGCAC	2 cut(s)	1425, 1477
ApeKI	GCWGC	6 cut(s)	174, 820, 952, 2086, 2261, 2372
ApoI	RAATTY	2 cut(s)	1329, 2426
AsiGI	ACCGGT	2 cut(s)	110, 254
Asp700I	GAANNNNTTC	3 cut(s)	248, 800, 2307
Asp718I	GGTACC	1 cut(s)	419
AspLEI	GCGC	3 cut(s)	622, 1015, 2471
AspS9I	GGNCC	5 cut(s)	304, 329, 638, 1250, 1312
AsuC2I	CCSGG	5 cut(s)	360, 720, 942, 943, 1089
AsuHPI	GGTGA	6 cut(s)	100, 244, 739, 1070, 1081, 2143
AsuII	TTCGAA	2 cut(s)	1683, 2191
AsuNHI	GCTAGC	1 cut(s)	2233
AvaI	CYCGRG	3 cut(s)	530, 862, 941
AvaII	GGWCC	3 cut(s)	304, 329, 1250
BaeGI	GKGCMC	2 cut(s)	1429, 1481
BamHI	GGATCC	1 cut(s)	1996
BanI	GGYRCC	2 cut(s)	419, 1399
BanII	GRGCYC	2 cut(s)	66, 1582
BarI	GAAGNNNNNNTAC	4 cut(s)	253, 285, 2090, 2122
BauI	CACGAG	1 cut(s)	1316
Bbv12I	GWGCWC	4 cut(s)	1429, 1481, 1582, 2389
BbvI	GCAGC	6 cut(s)	186, 832, 964, 2098, 2248, 2384
BceAI	ACGGC	2 cut(s)	652, 1917
BcgI	CGANNNNNNTGC	2 cut(s)	1369, 1403
BciT130I	CCWGG	1 cut(s)	308
BcnI	CCSGG	5 cut(s)	360, 720, 942, 943, 1089
BcoDI	GTCTC	4 cut(s)	577, 1301, 1777, 1966
BfaI	CTAG	7 cut(s)	164, 858, 912, 1058, 1985, 2082, 2234
BfmI	CTRYAG	3 cut(s)	1164, 2262, 2312
BfoI	RGCGCY	1 cut(s)	1016
BfrI	CTTAAG	1 cut(s)	1469
BfuAI	ACCTGC	1 cut(s)	274
BglII	AGATCT	1 cut(s)	1754
BisI	GCNGC	9 cut(s)	175, 349, 821, 953, 1235, 1519, 2087, 2262, 2373
BlpI	GCTNAGC	1 cut(s)	1804
BlsI	GCNGC	9 cut(s)	176, 350, 822, 954, 1236, 1520, 2088, 2263, 2374
BmcAI	AGTACT	2 cut(s)	828, 2006
Bme1390I	CCNGG	6 cut(s)	308, 360, 720, 942, 943, 1089
Bme18I	GGWCC	3 cut(s)	304, 329, 1250
BmeT110I	CYCGRG	3 cut(s)	530, 862, 941
BmgT120I	GGNCC	5 cut(s)	304, 329, 638, 1250, 1312
BmiI	GGNNCC	5 cut(s)	421, 839, 1252, 1401, 1998
BmrFI	CCNGG	6 cut(s)	308, 360, 720, 942, 943, 1089
BmrI	ACTGGG	3 cut(s)	332, 501, 1280
BmsI	GCATC	4 cut(s)	1689, 2357, 2384, 2456
BmtI	GCTAGC	1 cut(s)	2237
BmuI	ACTGGG	3 cut(s)	332, 501, 1280
BoxI	GACNNNNGTC	1 cut(s)	1970
BplI	GAGNNNNNCTC	2 cut(s)	1619, 1651
Bpu1102I	GCTNAGC	1 cut(s)	1804
Bpu14I	TTCGAA	2 cut(s)	1683, 2191
BpuMI	CCSGG	5 cut(s)	360, 720, 942, 943, 1089
BsaBI	GATNNNNATC	2 cut(s)	1131, 1568
BsaHI	GRCGYC	1 cut(s)	1605
BsaJI	CCNNGG	6 cut(s)	81, 358, 940, 941, 942, 2361
BsaWI	WCCGGW	2 cut(s)	110, 254
BsaXI	ACNNNNNCTCC	2 cut(s)	1744, 1774
Bsc4I	CCNNNNNNNGG	5 cut(s)	131, 992, 1023, 1166, 1241
Bse118I	RCCGGY	3 cut(s)	110, 254, 2441
Bse1I	ACTGG	7 cut(s)	331, 338, 496, 661, 1005, 1286, 1821
Bse3DI	GCAATG	4 cut(s)	54, 815, 856, 2249
Bse8I	GATNNNNATC	2 cut(s)	1131, 1568
BseBI	CCWGG	1 cut(s)	308
BseDI	CCNNGG	6 cut(s)	81, 358, 940, 941, 942, 2361
BseGI	GGATG	5 cut(s)	153, 926, 2331, 2395, 2471
BseJI	GATNNNNATC	2 cut(s)	1131, 1568
BseLI	CCNNNNNNNGG	5 cut(s)	131, 992, 1023, 1166, 1241
BseMI	GCAATG	4 cut(s)	54, 815, 856, 2249
BseMII	CTCAG	9 cut(s)	60, 131, 616, 977, 1179, 1818, 1982, 1992, 2211
BseNI	ACTGG	7 cut(s)	331, 338, 496, 661, 1005, 1286, 1821
BseRI	GAGGAG	3 cut(s)	565, 1795, 2165
BseSI	GKGCMC	2 cut(s)	1429, 1481
BseXI	GCAGC	6 cut(s)	186, 832, 964, 2098, 2248, 2384
Bsh1236I	CGCG	2 cut(s)	620, 1641
BshFI	GGCC	4 cut(s)	105, 639, 1314, 1923
BshNI	GGYRCC	2 cut(s)	419, 1399
BshTI	ACCGGT	2 cut(s)	110, 254
BsiHKAI	GWGCWC	4 cut(s)	1429, 1481, 1582, 2389
BsiHKCI	CYCGRG	3 cut(s)	530, 862, 941
BsiSI	CCGG	7 cut(s)	111, 255, 360, 719, 942, 1089, 2442
BslFI	GGGAC	4 cut(s)	508, 796, 1968, 2256
BslI	CCNNNNNNNGG	5 cut(s)	131, 992, 1023, 1166, 1241
BsmAI	GTCTC	4 cut(s)	577, 1301, 1777, 1966
BsmFI	GGGAC	4 cut(s)	508, 796, 1968, 2256
BsmI	GAATGC	1 cut(s)	670
BsnI	GGCC	4 cut(s)	105, 639, 1314, 1923
BsoBI	CYCGRG	3 cut(s)	530, 862, 941
Bsp119I	TTCGAA	2 cut(s)	1683, 2191
Bsp1286I	GDGCHC	5 cut(s)	66, 1429, 1481, 1582, 2389
Bsp1720I	GCTNAGC	1 cut(s)	1804
BspACI	CCGC	9 cut(s)	59, 348, 1235, 1358, 1407, 1422, 1518, 1947, 2460
BspANI	GGCC	4 cut(s)	105, 639, 1314, 1923
BspCNI	CTCAG	9 cut(s)	61, 132, 615, 976, 1180, 1817, 1981, 1993, 2210
BspFNI	CGCG	2 cut(s)	620, 1641
BspLI	GGNNCC	5 cut(s)	421, 839, 1252, 1401, 1998
BspMAI	CTGCAG	2 cut(s)	2266, 2316
BspMI	ACCTGC	1 cut(s)	274
BspOI	GCTAGC	1 cut(s)	2237
BspQI	GCTCTTC	2 cut(s)	780, 2027
BspT104I	TTCGAA	2 cut(s)	1683, 2191
BspT107I	GGYRCC	2 cut(s)	419, 1399
BspTI	CTTAAG	1 cut(s)	1469
BsrBI	CCGCTC	1 cut(s)	348
BsrDI	GCAATG	4 cut(s)	54, 815, 856, 2249
BsrFI	RCCGGY	3 cut(s)	110, 254, 2441
BsrI	ACTGG	7 cut(s)	331, 338, 496, 661, 1005, 1286, 1821
BssAI	RCCGGY	3 cut(s)	110, 254, 2441
BssECI	CCNNGG	6 cut(s)	81, 358, 940, 941, 942, 2361
BssNAI	GTATAC	2 cut(s)	263, 2044
BssNI	GRCGYC	1 cut(s)	1605
BssSI	CACGAG	1 cut(s)	1316
BssT1I	CCWWGG	1 cut(s)	81
Bst1107I	GTATAC	2 cut(s)	263, 2044
Bst2BI	CACGAG	1 cut(s)	1316
Bst2UI	CCWGG	1 cut(s)	308
Bst4CI	ACNGT	8 cut(s)	375, 787, 813, 875, 903, 1434, 1499, 2162
Bst6I	CTCTTC	3 cut(s)	780, 1772, 2027
BstACI	GRCGYC	1 cut(s)	1605
BstAFI	CTTAAG	1 cut(s)	1469
BstBI	TTCGAA	2 cut(s)	1683, 2191
BstC8I	GCNNGC	4 cut(s)	1544, 1836, 2235, 2414
BstDSI	CCRYGG	1 cut(s)	2361
BstENI	CCTNNNNNAGG	1 cut(s)	990
BstF5I	GGATG	5 cut(s)	153, 926, 2331, 2395, 2471
BstFNI	CGCG	2 cut(s)	620, 1641
BstH2I	RGCGCY	1 cut(s)	1016
BstHHI	GCGC	3 cut(s)	622, 1015, 2471
BstMAI	GTCTC	4 cut(s)	577, 1301, 1777, 1966
BstMWI	GCNNNNNNNGC	5 cut(s)	1231, 1364, 2239, 2466, 2477
BstNI	CCWGG	1 cut(s)	308
BstNSI	RCATGY	5 cut(s)	939, 1148, 1234, 1546, 1961
BstPAI	GACNNNNGTC	1 cut(s)	1970
BstSCI	CCNGG	6 cut(s)	306, 358, 718, 940, 941, 1087
BstSFI	CTRYAG	3 cut(s)	1164, 2262, 2312
BstSLI	GKGCMC	2 cut(s)	1429, 1481
BstUI	CGCG	2 cut(s)	620, 1641
BstV1I	GCAGC	6 cut(s)	186, 832, 964, 2098, 2248, 2384
BstX2I	RGATCY	7 cut(s)	77, 85, 136, 726, 1511, 1754, 1996
BstYI	RGATCY	7 cut(s)	77, 85, 136, 726, 1511, 1754, 1996
BstZ17I	GTATAC	2 cut(s)	263, 2044
BsuRI	GGCC	4 cut(s)	105, 639, 1314, 1923
BtgI	CCRYGG	1 cut(s)	2361
BtgZI	GCGATG	2 cut(s)	726, 2052
BtsCI	GGATG	5 cut(s)	153, 926, 2331, 2395, 2471
BtsIMutI	CAGTG	5 cut(s)	187, 455, 489, 998, 1101
BveI	ACCTGC	1 cut(s)	274
Cac8I	GCNNGC	4 cut(s)	1544, 1836, 2235, 2414
CaiI	CAGNNNCTG	1 cut(s)	1702
CfoI	GCGC	3 cut(s)	622, 1015, 2471
Cfr10I	RCCGGY	3 cut(s)	110, 254, 2441
Cfr13I	GGNCC	5 cut(s)	304, 329, 638, 1250, 1312
Cfr9I	CCCGGG	1 cut(s)	941
CseI	GACGC	2 cut(s)	1594, 2236
Csp6I	GTAC	6 cut(s)	420, 827, 871, 1142, 2005, 2123
CspAI	ACCGGT	2 cut(s)	110, 254
CspCI	CAANNNNNGTGG	2 cut(s)	2350, 2385
CviAII	CATG	9 cut(s)	464, 936, 1145, 1231, 1543, 1829, 1958, 2011, 2239
CviQI	GTAC	6 cut(s)	420, 827, 871, 1142, 2005, 2123
Eam1104I	CTCTTC	3 cut(s)	780, 1772, 2027
EarI	CTCTTC	3 cut(s)	780, 1772, 2027
Ecl136II	GAGCTC	1 cut(s)	1580
Eco130I	CCWWGG	1 cut(s)	81
Eco147I	AGGCCT	1 cut(s)	105
Eco24I	GRGCYC	2 cut(s)	66, 1582
Eco32I	GATATC	1 cut(s)	983
Eco47I	GGWCC	3 cut(s)	304, 329, 1250
Eco53kI	GAGCTC	1 cut(s)	1580
Eco57I	CTGAAG	1 cut(s)	163
Eco88I	CYCGRG	3 cut(s)	530, 862, 941
EcoICRI	GAGCTC	1 cut(s)	1580
EcoNI	CCTNNNNNAGG	1 cut(s)	990
EcoRI	GAATTC	1 cut(s)	2426
EcoRII	CCWGG	1 cut(s)	306
EcoRV	GATATC	1 cut(s)	983
EcoT14I	CCWWGG	1 cut(s)	81
EcoT38I	GRGCYC	2 cut(s)	66, 1582
ErhI	CCWWGG	1 cut(s)	81
FaeI	CATG	9 cut(s)	467, 939, 1148, 1234, 1546, 1832, 1961, 2014, 2242
FalI	AAGNNNNNCTT	4 cut(s)	209, 241, 1369, 1401
FaqI	GGGAC	4 cut(s)	508, 796, 1968, 2256
FatI	CATG	9 cut(s)	463, 935, 1144, 1230, 1542, 1828, 1957, 2010, 2238
FauI	CCCGC	1 cut(s)	52
FblI	GTMKAC	3 cut(s)	262, 681, 2043
Fnu4HI	GCNGC	9 cut(s)	175, 349, 821, 953, 1235, 1519, 2087, 2262, 2373
FokI	GGATG	5 cut(s)	140, 913, 2338, 2382, 2478
FriOI	GRGCYC	2 cut(s)	66, 1582
Fsp4HI	GCNGC	9 cut(s)	175, 349, 821, 953, 1235, 1519, 2087, 2262, 2373
FspBI	CTAG	7 cut(s)	164, 858, 912, 1058, 1985, 2082, 2234
GlaI	GCGC	3 cut(s)	621, 1014, 2470
GluI	GCNGC	9 cut(s)	175, 349, 821, 953, 1235, 1519, 2087, 2262, 2373
HaeII	RGCGCY	1 cut(s)	1016
HaeIII	GGCC	4 cut(s)	105, 639, 1314, 1923
HapII	CCGG	7 cut(s)	111, 255, 360, 719, 942, 1089, 2442
HgaI	GACGC	2 cut(s)	1594, 2236
HhaI	GCGC	3 cut(s)	622, 1015, 2471
Hin1I	GRCGYC	1 cut(s)	1605
Hin1II	CATG	9 cut(s)	467, 939, 1148, 1234, 1546, 1832, 1961, 2014, 2242
Hin6I	GCGC	3 cut(s)	620, 1013, 2469
HinP1I	GCGC	3 cut(s)	620, 1013, 2469
HincII	GTYRAC	2 cut(s)	324, 2175
HindII	GTYRAC	2 cut(s)	324, 2175
HindIII	AAGCTT	1 cut(s)	1741
HpaI	GTTAAC	1 cut(s)	2175
HpaII	CCGG	7 cut(s)	111, 255, 360, 719, 942, 1089, 2442
HphI	GGTGA	6 cut(s)	100, 244, 739, 1070, 1081, 2143
Hpy188III	TCNNGA	9 cut(s)	21, 157, 530, 1198, 1615, 1963, 2000, 2095, 2199
Hpy99I	CGWCG	2 cut(s)	1610, 2252
HpyAV	CCTTC	7 cut(s)	235, 986, 1264, 1522, 1900, 2092, 2287
HpyCH4III	ACNGT	8 cut(s)	375, 787, 813, 875, 903, 1434, 1499, 2162
HpyCH4IV	ACGT	3 cut(s)	30, 1354, 2406
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1231, 1364, 2239, 2466, 2477
HpySE526I	ACGT	3 cut(s)	30, 1354, 2406
Hsp92I	GRCGYC	1 cut(s)	1605
Hsp92II	CATG	9 cut(s)	467, 939, 1148, 1234, 1546, 1832, 1961, 2014, 2242
HspAI	GCGC	3 cut(s)	620, 1013, 2469
KpnI	GGTACC	1 cut(s)	423
KspAI	GTTAAC	1 cut(s)	2175
LguI	GCTCTTC	2 cut(s)	780, 2027
LmnI	GCTCC	6 cut(s)	449, 721, 1442, 2152, 2230, 2392
Lsp1109I	GCAGC	6 cut(s)	186, 832, 964, 2098, 2248, 2384
LweI	GCATC	4 cut(s)	1689, 2357, 2384, 2456
MaeI	CTAG	7 cut(s)	164, 858, 912, 1058, 1985, 2082, 2234
MaeII	ACGT	3 cut(s)	30, 1354, 2406
MaeIII	GTNAC	6 cut(s)	50, 605, 689, 1039, 1098, 1909
MbiI	CCGCTC	1 cut(s)	348
MboII	GAAGA	9 cut(s)	530, 767, 776, 808, 1108, 1789, 2044, 2264, 2343
MfeI	CAATTG	3 cut(s)	1771, 2021, 2126
MflI	RGATCY	7 cut(s)	77, 85, 136, 726, 1511, 1754, 1996
MhlI	GDGCHC	5 cut(s)	66, 1429, 1481, 1582, 2389
MlyI	GAGTC	7 cut(s)	520, 874, 1036, 1779, 1960, 2008, 2064
MmeI	TCCRAC	3 cut(s)	312, 612, 704
MroXI	GAANNNNTTC	3 cut(s)	248, 800, 2307
MseI	TTAA	9 cut(s)	74, 611, 1137, 1179, 1376, 1436, 1470, 1898, 2174
MslI	CAYNNNNRTG	2 cut(s)	1653, 1860
MspA1I	CMGCKG	3 cut(s)	1947, 2261, 2460
MspCI	CTTAAG	1 cut(s)	1469
MspI	CCGG	7 cut(s)	111, 255, 360, 719, 942, 1089, 2442
MspR9I	CCNGG	6 cut(s)	308, 360, 720, 942, 943, 1089
MunI	CAATTG	3 cut(s)	1771, 2021, 2126
Mva1269I	GAATGC	1 cut(s)	670
MvaI	CCWGG	1 cut(s)	308
MvnI	CGCG	2 cut(s)	620, 1641
MwoI	GCNNNNNNNGC	5 cut(s)	1231, 1364, 2239, 2466, 2477
NciI	CCSGG	5 cut(s)	360, 720, 942, 943, 1089
NheI	GCTAGC	1 cut(s)	2233
NlaIII	CATG	9 cut(s)	467, 939, 1148, 1234, 1546, 1832, 1961, 2014, 2242
NlaIV	GGNNCC	5 cut(s)	421, 839, 1252, 1401, 1998
NmeAIII	GCCGAG	1 cut(s)	1561
NmuCI	GTSAC	3 cut(s)	50, 1039, 1098
NspI	RCATGY	5 cut(s)	939, 1148, 1234, 1546, 1961
NspV	TTCGAA	2 cut(s)	1683, 2191
PaeI	GCATGC	1 cut(s)	1546
PaeR7I	CTCGAG	2 cut(s)	530, 862
PceI	AGGCCT	1 cut(s)	105
PciI	ACATGT	2 cut(s)	1144, 1957
PciSI	GCTCTTC	2 cut(s)	780, 2027
PcsI	WCGNNNNNNNCGW	1 cut(s)	2188
PctI	GAATGC	1 cut(s)	670
PdmI	GAANNNNTTC	3 cut(s)	248, 800, 2307
PfeI	GAWTC	3 cut(s)	486, 1674, 2255
PinAI	ACCGGT	2 cut(s)	110, 254
PkrI	GCNGC	9 cut(s)	176, 350, 822, 954, 1236, 1520, 2088, 2263, 2374
PleI	GAGTC	7 cut(s)	520, 873, 1036, 1779, 1960, 2008, 2063
PpsI	GAGTC	7 cut(s)	520, 873, 1036, 1779, 1960, 2008, 2063
PscI	ACATGT	2 cut(s)	1144, 1957
PshAI	GACNNNNGTC	1 cut(s)	1970
Psp124BI	GAGCTC	1 cut(s)	1582
Psp1406I	AACGTT	1 cut(s)	2406
Psp6I	CCWGG	1 cut(s)	306
PspGI	CCWGG	1 cut(s)	306
PspN4I	GGNNCC	5 cut(s)	421, 839, 1252, 1401, 1998
PspPI	GGNCC	5 cut(s)	304, 329, 638, 1250, 1312
PspXI	VCTCGAGB	1 cut(s)	862
PstI	CTGCAG	2 cut(s)	2266, 2316
PstNI	CAGNNNCTG	1 cut(s)	1702
PsuI	RGATCY	7 cut(s)	77, 85, 136, 726, 1511, 1754, 1996
PvuII	CAGCTG	1 cut(s)	2261
RsaI	GTAC	6 cut(s)	421, 828, 872, 1143, 2006, 2124
RsaNI	GTAC	6 cut(s)	420, 827, 871, 1142, 2005, 2123
RseI	CAYNNNNRTG	2 cut(s)	1653, 1860
SacI	GAGCTC	1 cut(s)	1582
SapI	GCTCTTC	2 cut(s)	780, 2027
SaqAI	TTAA	9 cut(s)	74, 611, 1137, 1179, 1376, 1436, 1470, 1898, 2174
SatI	GCNGC	9 cut(s)	175, 349, 821, 953, 1235, 1519, 2087, 2262, 2373
Sau96I	GGNCC	5 cut(s)	304, 329, 638, 1250, 1312
ScaI	AGTACT	2 cut(s)	828, 2006
SchI	GAGTC	7 cut(s)	520, 874, 1036, 1779, 1960, 2008, 2064
ScrFI	CCNGG	6 cut(s)	308, 360, 720, 942, 943, 1089
SduI	GDGCHC	5 cut(s)	66, 1429, 1481, 1582, 2389
SfaNI	GCATC	4 cut(s)	1689, 2357, 2384, 2456
SfcI	CTRYAG	3 cut(s)	1164, 2262, 2312
Sfr274I	CTCGAG	2 cut(s)	530, 862
SfuI	TTCGAA	2 cut(s)	1683, 2191
SinI	GGWCC	3 cut(s)	304, 329, 1250
SlaI	CTCGAG	2 cut(s)	530, 862
SmaI	CCCGGG	1 cut(s)	943
SmiMI	CAYNNNNRTG	2 cut(s)	1653, 1860
SmlI	CTYRAG	3 cut(s)	530, 862, 1469
SmoI	CTYRAG	3 cut(s)	530, 862, 1469
SphI	GCATGC	1 cut(s)	1546
SseBI	AGGCCT	1 cut(s)	105
SsiI	CCGC	9 cut(s)	59, 348, 1235, 1358, 1407, 1422, 1518, 1947, 2460
SspI	AATATT	2 cut(s)	513, 1879
SspMI	CTAG	7 cut(s)	164, 858, 912, 1058, 1985, 2082, 2234
SstI	GAGCTC	1 cut(s)	1582
StuI	AGGCCT	1 cut(s)	105
StyD4I	CCNGG	6 cut(s)	306, 358, 718, 940, 941, 1087
StyI	CCWWGG	1 cut(s)	81
TaaI	ACNGT	8 cut(s)	375, 787, 813, 875, 903, 1434, 1499, 2162
TaiI	ACGT	3 cut(s)	33, 1357, 2409
TaqII	GACCGA	1 cut(s)	2042
TatI	WGTACW	3 cut(s)	826, 1141, 2004
TauI	GCSGC	3 cut(s)	351, 1237, 1521
TfiI	GAWTC	3 cut(s)	486, 1674, 2255
Tru1I	TTAA	9 cut(s)	74, 611, 1137, 1179, 1376, 1436, 1470, 1898, 2174
Tru9I	TTAA	9 cut(s)	74, 611, 1137, 1179, 1376, 1436, 1470, 1898, 2174
TscAI	CASTG	5 cut(s)	187, 462, 496, 1005, 1101
TseFI	GTSAC	3 cut(s)	50, 1039, 1098
TseI	GCWGC	6 cut(s)	174, 820, 952, 2086, 2261, 2372
Tsp45I	GTSAC	3 cut(s)	50, 1039, 1098
TspDTI	ATGAA	8 cut(s)	41, 81, 867, 1634, 1754, 1905, 2342, 2343
TspGWI	ACGGA	5 cut(s)	508, 858, 932, 2105, 2378
TspMI	CCCGGG	1 cut(s)	941
TspRI	CASTG	5 cut(s)	187, 462, 496, 1005, 1101
Vha464I	CTTAAG	1 cut(s)	1469
VneI	GTGCAC	2 cut(s)	1425, 1477
VpaK11BI	GGWCC	3 cut(s)	304, 329, 1250
XagI	CCTNNNNNAGG	1 cut(s)	990
XapI	RAATTY	2 cut(s)	1329, 2426
XceI	RCATGY	5 cut(s)	939, 1148, 1234, 1546, 1961
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1796
XhoI	CTCGAG	2 cut(s)	530, 862
XmaI	CCCGGG	1 cut(s)	941
XmiI	GTMKAC	3 cut(s)	262, 681, 2043
XmnI	GAANNNNTTC	3 cut(s)	248, 800, 2307
XspI	CTAG	7 cut(s)	164, 858, 912, 1058, 1985, 2082, 2234
ZrmI	AGTACT	2 cut(s)	828, 2006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.