MD12G1196600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003096
Physical Location & Seq
Reverse (-)
27772573 .. 27778391
5819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1196600.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGGCAACCATGCGGTTGCTGCTGCTCTTTCTGGCTTTGTGCTTTTCTAGGCTTCATGTGATCTCCTCTGTTACAGACCCGGATGATGGTATTGCACTCCAATCCTTGAAGCAGACATGGAGCAATGTTCCACCCAGCTGGGACAAGTCAAGCGATCCGTGTGGGGCGCAATGGGAAGGCGTCACTTGCAACGACTCGAGGGTGACTGCATTGGGATTATCAGCAATAAACGTGAAAGGGAAAATTGAAGGCGACATCGGGGGCCTCTCTGAATTAAGATCCTTGGATCTTTCATTCAACAAAGGTCTCACCGGTTCTCTCTCTCCGCGATTAGGGGATCTGAGCAAGCTAAACATCCTGATCCTAGCTGGTTGCAGCTTCACTGGAAGTATTCCGAGTGAATTGGGGAATCTAAAAGAGCTAACCTTCTTGGCTTTGAATACGAATAACTTCACCGGTAGCATACCTGCTTCTTTTGGTAAACTCTCCAACCTCTACTGGCTGGACCTGGCAGACAATCAGTTGACTGGACCTATCCCAGTTTCAACCGCCGTTGCTCCCGGCTTAGACCAACTGGTGAAGGCTAAACATTTCCATTTCAACCAGAACCAGCTTTCAGGTATCATTCCAGCCAGACTTTTCAGCTCCAAGATGGTACTGATCCATATATTGTTTGATGGAAATAGATTCAGTGGGACTATTCCATCAACAATATTTTCTGTTCAGACTCTTGAGGTTCTTCGGCTTGATAGAAATGCTCTGACGGGACCAGTCTCCTCAAATATCACAAACCTTATAAATCTCGGTGAACTAAATTTGGCCTACAACGATTTGAATGGCTCTTTACCCGACTTAACTGGATTGAATTCCCTCAATTATGTAGACCTTAGTAACAACTCCTTTGATCCATCAGAAGCTCCGCTTTGGTTCTCAACCTTACCCTCGCTCACCACTATCGTTTTAGAATATGGAGCACTTCAAGGGACCGTGCCAGAGAAGATGTTCAGCATCCCGTCATTGGAGCAAGTGAAACTGAAAAACAATGCGTTCAACGATACATTAAACATGGGTGATAGTATCAGTCAGCAACTGACGCTTGTTGATTTGCAGAACAATGATATTCCCAGAATAACTCTGGGTTATGAATACCAAAATACATTAATACTTGTGGGGAACCCGGTGTGCACCAATGGGTCAAGCTCAAATGCTTTCTGTCAGCTTCCACAGCCAAATACGAAACCATATACTACCAGCACGAATTGTCCATCTGCTACATGCCCCGGGGGTCAGCAGCAAAGCCCTCAGAGTTGTCAATGTGGATATCCTTTCGAAGGAACATTGTATTTCCGAGCGCCCTCTTTCAGGGATTTGACAAATGTGAATCTGTTTCACTCACTAGAAATGAGCATATGGGTGAAACTTGGCCTCACTCCCGGTTCAGTGTCGCTTGAAAACCCTTTCTTCGACATCAATGACTATCTTCAAATACATCTGGCACTCTTTCCATCTTCAGGAACACATTTTAATAGGTCAGAGGTTATCAGGATTGGTTTTGATTTGAGCAATCAGACTTACAAGCCGCCGAAGGAGTTTGGACCCTACATTTTTATTCCAGCTCAATATAATTTCCCAGACGCGCGTAAAAGCTCTATGAGCACTGGTGTTATTATTGGGATATCAGCTGGTTGTCTCGTTCTTGTTCTGGGCCTTGTGGTATTAGGAATTTACGCCATTAGGCAAAAGAAACGTGCGGAAAGAGCCATTGGATTAAGCAGACCTTTCGCTTCTTGGGCGCCAAGCGGAAAAGATAGTGGCGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCGTATGATGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAGTAATGAAATAGGATCTGGTGGCTACGGGAAGGTTTATAGGGGCATCCTTTCTGATGGACAAGTAATAGCAATCAAAAAAGCTCAGCAAGGATCAATGCAGGGCGGCCTTGAGTTCAAGACTGAAATTGAGTTGCTCTCGCGCGTTCATCACAAAAATGTGGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTTTATGCCTAATGGAACGCTTAGGGAAAGCTTGTTGGGGAGATCTGGTATTTATCTCGATTGGAAGAGGAGACTCCGAATCACTCTTGGCTCGGCAAGAGGACTGGCTTACTTACACGAGCTTGCGAATCCTCCTATAATCCACAGAGATGTTAAGTCCTCCAATATTTTATTAGATGAACATTTAACTGCGAAGGTTGCAGATTTTGGCTTGTCCAAGTTGATCTCTGACAGCGGAAAAGGACATGTCTCGACTCAGGTCAAAGGCACACTGGGATATCTGGATCCTGAGTACTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCAACCGATTGAGAAGGGAAAATACATTGTCCGCGAGGTACGATTAGTGATGGACAAGAATGACGAAGAGCATTGCGGTTTGGGGGACTTAATAGACCGAAGCATTCGAAACTCAGGGACTCTCATCGGGTTTGGGAGGTTCTTGGAGCTAGCCATGCAATGCGTCGAAGATTCAGCTGCAGATCGTCCCACAATGAGTGAACTTGTGAAGGCTATCGAAACCATTCTGCAGAATGATGGGATGAACACAAACTCGACATCAGCATCTTCTTCGGCCACGGATTTTGCAACATCAAAAGGTGCTCCGAAACATCCCTACAACGATGGCTTGCCGAAAAAGGATGTCAATGACAGCTCCGGCACCTTTGATTACAGCGGTGGATATGCAGTTTCAGCGAAGATCGAACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase_Tyr

Protein Analysis

964

Amino Acids

105.0

Weight (kDa)

6.07

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 64 2.6e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 171 8.5e-06 Leucine-rich repeat region
Pkinase PF00069 629 - 896 4.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 632 - 897 2.5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 475
AccB1I GGYRCC 2 cut(s) 1792, 2837
AccI GTMKAC 2 cut(s) 882, 2436
AccII CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
AcoI YGGCCR 1 cut(s) 2751
AcsI RAATTY 3 cut(s) 814, 865, 1722
AcuI CTGAAG 1 cut(s) 1494
AcyI GRCGYC 2 cut(s) 180, 1793
AfaI GTAC 3 cut(s) 657, 2399, 2517
AfiI CCNNNNNNNGG 6 cut(s) 86, 332, 1018, 1331, 1362, 1511
AflII CTTAAG 1 cut(s) 1862
AflIII ACRYGT 1 cut(s) 2350
AgeI ACCGGT 2 cut(s) 311, 455
AjnI CCWGG 1 cut(s) 507
AjuI GAANNNNNNNTTGG 2 cut(s) 1746, 1778
Alw21I GWGCWC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
Alw26I GTCTC 5 cut(s) 311, 778, 1694, 2170, 2359
Alw44I GTGCAC 3 cut(s) 1183, 1818, 1870
AlwNI CAGNNNCTG 2 cut(s) 1090, 2095
Ama87I CYCGRG 2 cut(s) 196, 1280
AoxI GGCC 6 cut(s) 262, 819, 1423, 1705, 1996, 2751
ApaLI GTGCAC 3 cut(s) 1183, 1818, 1870
ApeKI GCWGC 5 cut(s) 19, 22, 375, 1291, 2654
ApoI RAATTY 3 cut(s) 814, 865, 1722
AseI ATTAAT 1 cut(s) 1160
AsiGI ACCGGT 2 cut(s) 311, 455
Asp700I GAANNNNTTC 3 cut(s) 449, 1001, 2700
AspLEI GCGC 5 cut(s) 169, 1354, 1639, 1795, 2034
AspS9I GGNCC 7 cut(s) 262, 505, 530, 767, 984, 1595, 1705
AsuC2I CCSGG 6 cut(s) 80, 561, 1178, 1281, 1282, 1434
AsuHPI GGTGA 8 cut(s) 214, 301, 445, 589, 818, 940, 1082, 1426
AsuII TTCGAA 3 cut(s) 1329, 2076, 2584
AsuNHI GCTAGC 1 cut(s) 2626
AvaI CYCGRG 2 cut(s) 196, 1280
AvaII GGWCC 5 cut(s) 505, 530, 767, 984, 1595
BaeGI GKGCMC 3 cut(s) 1187, 1822, 1874
BamHI GGATCC 1 cut(s) 2389
BanI GGYRCC 2 cut(s) 1792, 2837
BarI GAAGNNNNNNTAC 2 cut(s) 2483, 2515
BauI CACGAG 1 cut(s) 2222
Bbv12I GWGCWC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
BbvI GCAGC 5 cut(s) 6, 9, 387, 1303, 2641
BceAI ACGGC 1 cut(s) 536
BcgI CGANNNNNNTGC 4 cut(s) 1762, 1796, 2730, 2764
BciT130I CCWGG 1 cut(s) 509
BcnI CCSGG 6 cut(s) 80, 561, 1178, 1281, 1282, 1434
BcoDI GTCTC 5 cut(s) 311, 778, 1694, 2170, 2359
BfaI CTAG 5 cut(s) 48, 365, 1397, 2475, 2627
BfmI CTRYAG 2 cut(s) 2655, 2705
BfoI RGCGCY 2 cut(s) 1355, 1796
BfrI CTTAAG 1 cut(s) 1862
BfuAI ACCTGC 1 cut(s) 475
BglII AGATCT 1 cut(s) 2147
BisI GCNGC 7 cut(s) 20, 23, 376, 1292, 1580, 1996, 2655
BlpI GCTNAGC 1 cut(s) 1974
BlsI GCNGC 7 cut(s) 21, 24, 377, 1293, 1581, 1997, 2656
BmcAI AGTACT 1 cut(s) 2399
Bme1390I CCNGG 7 cut(s) 80, 509, 561, 1178, 1281, 1282, 1434
Bme18I GGWCC 5 cut(s) 505, 530, 767, 984, 1595
BmeT110I CYCGRG 2 cut(s) 196, 1280
BmgT120I GGNCC 7 cut(s) 262, 505, 530, 767, 984, 1595, 1705
BmiI GGNNCC 8 cut(s) 263, 768, 985, 1175, 1597, 1794, 2391, 2839
BmrFI CCNGG 7 cut(s) 80, 509, 561, 1178, 1281, 1282, 1434
BmrI ACTGGG 2 cut(s) 533, 2387
BmsI GCATC 4 cut(s) 1017, 1944, 2082, 2750
BmtI GCTAGC 1 cut(s) 2630
BmuI ACTGGG 2 cut(s) 533, 2387
BoxI GACNNNNGTC 1 cut(s) 2363
BplI GAGNNNNNCTC 2 cut(s) 2012, 2044
Bpu10I CCTNAGC 1 cut(s) 2126
Bpu1102I GCTNAGC 1 cut(s) 1974
Bpu14I TTCGAA 3 cut(s) 1329, 2076, 2584
BpuEI CTTGAG 2 cut(s) 752, 2021
BpuMI CCSGG 6 cut(s) 80, 561, 1178, 1281, 1282, 1434
BsaHI GRCGYC 2 cut(s) 180, 1793
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 5 cut(s) 282, 1279, 1280, 1281, 2754
BsaWI WCCGGW 2 cut(s) 311, 455
BsaXI ACNNNNNCTCC 2 cut(s) 2137, 2167
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 332, 1018, 1331, 1362, 1511
Bse118I RCCGGY 2 cut(s) 311, 455
Bse3DI GCAATG 4 cut(s) 130, 176, 2548, 2642
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 5 cut(s) 282, 1279, 1280, 1281, 2754
BseGI GGATG 7 cut(s) 88, 354, 1008, 1935, 2724, 2788, 2824
BseLI CCNNNNNNNGG 6 cut(s) 86, 332, 1018, 1331, 1362, 1511
BseMI GCAATG 4 cut(s) 130, 176, 2548, 2642
BseMII CTCAG 6 cut(s) 332, 1316, 1988, 2375, 2385, 2604
BseRI GAGGAG 3 cut(s) 55, 766, 2188
BseSI GKGCMC 3 cut(s) 1187, 1822, 1874
BseXI GCAGC 5 cut(s) 6, 9, 387, 1303, 2641
BseYI CCCAGC 2 cut(s) 134, 138
Bsh1236I CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
BshFI GGCC 6 cut(s) 264, 821, 1425, 1707, 1998, 2753
BshNI GGYRCC 2 cut(s) 1792, 2837
BshTI ACCGGT 2 cut(s) 311, 455
BsiHKAI GWGCWC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
BsiHKCI CYCGRG 2 cut(s) 196, 1280
BsiSI CCGG 8 cut(s) 80, 312, 456, 561, 1178, 1281, 1434, 2835
BslFI GGGAC 7 cut(s) 155, 709, 780, 997, 2576, 2608, 2649
BslI CCNNNNNNNGG 6 cut(s) 86, 332, 1018, 1331, 1362, 1511
BsmAI GTCTC 5 cut(s) 311, 778, 1694, 2170, 2359
BsmFI GGGAC 7 cut(s) 155, 709, 780, 997, 2576, 2608, 2649
BsmI GAATGC 1 cut(s) 2580
BsnI GGCC 6 cut(s) 264, 821, 1425, 1707, 1998, 2753
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 2 cut(s) 196, 1280
Bsp119I TTCGAA 3 cut(s) 1329, 2076, 2584
Bsp1286I GDGCHC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
Bsp1720I GCTNAGC 1 cut(s) 1974
BspANI GGCC 6 cut(s) 264, 821, 1425, 1707, 1998, 2753
BspCNI CTCAG 6 cut(s) 333, 1315, 1987, 2374, 2386, 2603
BspFNI CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
BspLI GGNNCC 8 cut(s) 263, 768, 985, 1175, 1597, 1794, 2391, 2839
BspMAI CTGCAG 2 cut(s) 2659, 2709
BspMI ACCTGC 1 cut(s) 475
BspOI GCTAGC 1 cut(s) 2630
BspQI GCTCTTC 2 cut(s) 2420, 2538
BspT104I TTCGAA 3 cut(s) 1329, 2076, 2584
BspT107I GGYRCC 2 cut(s) 1792, 2837
BspTI CTTAAG 1 cut(s) 1862
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 4 cut(s) 130, 176, 2548, 2642
BsrFI RCCGGY 2 cut(s) 311, 455
BssAI RCCGGY 2 cut(s) 311, 455
BssECI CCNNGG 5 cut(s) 282, 1279, 1280, 1281, 2754
BssNAI GTATAC 1 cut(s) 2437
BssNI GRCGYC 2 cut(s) 180, 1793
BssSI CACGAG 1 cut(s) 2222
BssT1I CCWWGG 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 2437
Bst2BI CACGAG 1 cut(s) 2222
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 988, 1827, 1892
Bst6I CTCTTC 3 cut(s) 2165, 2420, 2538
BstACI GRCGYC 2 cut(s) 180, 1793
BstAFI CTTAAG 1 cut(s) 1862
BstBI TTCGAA 3 cut(s) 1329, 2076, 2584
BstC8I GCNNGC 4 cut(s) 347, 2229, 2628, 2807
BstDEI CTNAG 9 cut(s) 341, 565, 887, 1302, 1974, 2126, 2361, 2394, 2590
BstDSI CCRYGG 1 cut(s) 2754
BstENI CCTNNNNNAGG 2 cut(s) 1329, 1360
BstF5I GGATG 7 cut(s) 88, 354, 1008, 1935, 2724, 2788, 2824
BstFNI CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
BstH2I RGCGCY 2 cut(s) 1355, 1796
BstHHI GCGC 5 cut(s) 169, 1354, 1639, 1795, 2034
BstMAI GTCTC 5 cut(s) 311, 778, 1694, 2170, 2359
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 2 cut(s) 1278, 2354
BstPAI GACNNNNGTC 1 cut(s) 2363
BstSCI CCNGG 7 cut(s) 78, 507, 559, 1176, 1279, 1280, 1432
BstSFI CTRYAG 2 cut(s) 2655, 2705
BstSLI GKGCMC 3 cut(s) 1187, 1822, 1874
BstUI CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
BstV1I GCAGC 5 cut(s) 6, 9, 387, 1303, 2641
BstX2I RGATCY 6 cut(s) 278, 286, 337, 1904, 2147, 2389
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 6 cut(s) 278, 286, 337, 1904, 2147, 2389
BstZ17I GTATAC 1 cut(s) 2437
BsuRI GGCC 6 cut(s) 264, 821, 1425, 1707, 1998, 2753
BtgI CCRYGG 1 cut(s) 2754
BtgZI GCGATG 1 cut(s) 2445
BtsCI GGATG 7 cut(s) 88, 354, 1008, 1935, 2724, 2788, 2824
BtsIMutI CAGTG 5 cut(s) 381, 697, 1446, 1656, 2375
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 4 cut(s) 347, 2229, 2628, 2807
CaiI CAGNNNCTG 2 cut(s) 1090, 2095
CfoI GCGC 5 cut(s) 169, 1354, 1639, 1795, 2034
Cfr10I RCCGGY 2 cut(s) 311, 455
Cfr13I GGNCC 7 cut(s) 262, 505, 530, 767, 984, 1595, 1705
Cfr9I CCCGGG 1 cut(s) 1280
CseI GACGC 4 cut(s) 169, 1102, 1643, 2629
Csp6I GTAC 3 cut(s) 656, 2398, 2516
CspAI ACCGGT 2 cut(s) 311, 455
CspCI CAANNNNNGTGG 2 cut(s) 2743, 2778
CviAII CATG 8 cut(s) 10, 56, 117, 1066, 1275, 2351, 2404, 2632
CviQI GTAC 3 cut(s) 656, 2398, 2516
DdeI CTNAG 9 cut(s) 341, 565, 887, 1302, 1974, 2126, 2361, 2394, 2590
DinI GGCGCC 1 cut(s) 1794
EaeI YGGCCR 1 cut(s) 2751
Eam1104I CTCTTC 3 cut(s) 2165, 2420, 2538
EarI CTCTTC 3 cut(s) 2165, 2420, 2538
Eco130I CCWWGG 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 311
Eco32I GATATC 3 cut(s) 1322, 1677, 2384
Eco47I GGWCC 5 cut(s) 505, 530, 767, 984, 1595
Eco57I CTGAAG 1 cut(s) 1494
Eco88I CYCGRG 2 cut(s) 196, 1280
EcoNI CCTNNNNNAGG 2 cut(s) 1329, 1360
EcoO109I RGGNCCY 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 3 cut(s) 1322, 1677, 2384
EcoT14I CCWWGG 1 cut(s) 282
EgeI GGCGCC 1 cut(s) 1794
EheI GGCGCC 1 cut(s) 1794
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 8 cut(s) 13, 59, 120, 1069, 1278, 2354, 2407, 2635
FalI AAGNNNNNCTT 4 cut(s) 906, 938, 1762, 1794
FaqI GGGAC 7 cut(s) 155, 709, 780, 997, 2576, 2608, 2649
FatI CATG 8 cut(s) 9, 55, 116, 1065, 1274, 2350, 2403, 2631
FauNDI CATATG 1 cut(s) 1409
FblI GTMKAC 2 cut(s) 882, 2436
Fnu4HI GCNGC 7 cut(s) 20, 23, 376, 1292, 1580, 1996, 2655
FokI GGATG 7 cut(s) 95, 341, 995, 1922, 2731, 2775, 2831
Fsp4HI GCNGC 7 cut(s) 20, 23, 376, 1292, 1580, 1996, 2655
FspBI CTAG 5 cut(s) 48, 365, 1397, 2475, 2627
GlaI GCGC 5 cut(s) 168, 1353, 1638, 1794, 2033
GluI GCNGC 7 cut(s) 20, 23, 376, 1292, 1580, 1996, 2655
GsaI CCCAGC 2 cut(s) 138, 142
HaeII RGCGCY 2 cut(s) 1355, 1796
HaeIII GGCC 6 cut(s) 264, 821, 1425, 1707, 1998, 2753
HapII CCGG 8 cut(s) 80, 312, 456, 561, 1178, 1281, 1434, 2835
HgaI GACGC 4 cut(s) 169, 1102, 1643, 2629
HhaI GCGC 5 cut(s) 169, 1354, 1639, 1795, 2034
Hin1I GRCGYC 2 cut(s) 180, 1793
Hin1II CATG 8 cut(s) 13, 59, 120, 1069, 1278, 2354, 2407, 2635
Hin6I GCGC 5 cut(s) 167, 1352, 1637, 1793, 2032
HinP1I GCGC 5 cut(s) 167, 1352, 1637, 1793, 2032
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 2134
HpaII CCGG 8 cut(s) 80, 312, 456, 561, 1178, 1281, 1434, 2835
HphI GGTGA 8 cut(s) 214, 301, 445, 589, 818, 940, 1082, 1426
Hpy188III TCNNGA 9 cut(s) 358, 731, 1512, 1543, 2008, 2162, 2356, 2387, 2393
Hpy99I CGWCG 1 cut(s) 2645
HpyCH4III ACNGT 3 cut(s) 988, 1827, 1892
HpyCH4IV ACGT 2 cut(s) 231, 1747
HpyF3I CTNAG 9 cut(s) 341, 565, 887, 1302, 1974, 2126, 2361, 2394, 2590
HpySE526I ACGT 2 cut(s) 231, 1747
Hsp92I GRCGYC 2 cut(s) 180, 1793
Hsp92II CATG 8 cut(s) 13, 59, 120, 1069, 1278, 2354, 2407, 2635
HspAI GCGC 5 cut(s) 167, 1352, 1637, 1793, 2032
KasI GGCGCC 1 cut(s) 1792
LguI GCTCTTC 2 cut(s) 2420, 2538
Lsp1109I GCAGC 5 cut(s) 6, 9, 387, 1303, 2641
LweI GCATC 4 cut(s) 1017, 1944, 2082, 2750
MaeI CTAG 5 cut(s) 48, 365, 1397, 2475, 2627
MaeII ACGT 2 cut(s) 231, 1747
MaeIII GTNAC 4 cut(s) 70, 181, 202, 890
MfeI CAATTG 1 cut(s) 2414
MflI RGATCY 6 cut(s) 278, 286, 337, 1904, 2147, 2389
MhlI GDGCHC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
Mly113I GGCGCC 1 cut(s) 1793
MlyI GAGTC 7 cut(s) 188, 721, 2172, 2353, 2401, 2457, 2590
MmeI TCCRAC 1 cut(s) 513
MroXI GAANNNNTTC 3 cut(s) 449, 1001, 2700
MslI CAYNNNNRTG 3 cut(s) 1412, 2046, 2253
MspA1I CMGCKG 5 cut(s) 138, 1682, 2340, 2654, 2853
MspCI CTTAAG 1 cut(s) 1862
MspI CCGG 8 cut(s) 80, 312, 456, 561, 1178, 1281, 1434, 2835
MspR9I CCNGG 7 cut(s) 80, 509, 561, 1178, 1281, 1282, 1434
MunI CAATTG 1 cut(s) 2414
Mva1269I GAATGC 1 cut(s) 2580
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 6 cut(s) 328, 1637, 1639, 2032, 2034, 2511
NarI GGCGCC 1 cut(s) 1793
NciI CCSGG 6 cut(s) 80, 561, 1178, 1281, 1282, 1434
NdeI CATATG 1 cut(s) 1409
NheI GCTAGC 1 cut(s) 2626
NlaIII CATG 8 cut(s) 13, 59, 120, 1069, 1278, 2354, 2407, 2635
NlaIV GGNNCC 8 cut(s) 263, 768, 985, 1175, 1597, 1794, 2391, 2839
NmeAIII GCCGAG 1 cut(s) 2177
NmuCI GTSAC 2 cut(s) 181, 202
NspI RCATGY 2 cut(s) 1278, 2354
NspV TTCGAA 3 cut(s) 1329, 2076, 2584
PaeR7I CTCGAG 1 cut(s) 196
PciI ACATGT 1 cut(s) 2350
PciSI GCTCTTC 2 cut(s) 2420, 2538
PctI GAATGC 1 cut(s) 2580
PdmI GAANNNNTTC 3 cut(s) 449, 1001, 2700
PfeI GAWTC 7 cut(s) 409, 687, 1381, 2067, 2184, 2233, 2648
PinAI ACCGGT 2 cut(s) 311, 455
PkrI GCNGC 7 cut(s) 21, 24, 377, 1293, 1581, 1997, 2656
PleI GAGTC 7 cut(s) 188, 721, 2172, 2353, 2401, 2456, 2590
PluTI GGCGCC 1 cut(s) 1796
PpsI GAGTC 7 cut(s) 188, 721, 2172, 2353, 2401, 2456, 2590
PscI ACATGT 1 cut(s) 2350
PshAI GACNNNNGTC 1 cut(s) 2363
PshBI ATTAAT 1 cut(s) 1160
PsiI TTATAA 1 cut(s) 797
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 2 cut(s) 134, 138
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 8 cut(s) 263, 768, 985, 1175, 1597, 1794, 2391, 2839
PspPI GGNCC 7 cut(s) 262, 505, 530, 767, 984, 1595, 1705
PspXI VCTCGAGB 1 cut(s) 196
PstI CTGCAG 2 cut(s) 2659, 2709
PstNI CAGNNNCTG 2 cut(s) 1090, 2095
PsuI RGATCY 6 cut(s) 278, 286, 337, 1904, 2147, 2389
PvuII CAGCTG 3 cut(s) 138, 1682, 2654
RsaI GTAC 3 cut(s) 657, 2399, 2517
RsaNI GTAC 3 cut(s) 656, 2398, 2516
RseI CAYNNNNRTG 3 cut(s) 1412, 2046, 2253
SapI GCTCTTC 2 cut(s) 2420, 2538
SatI GCNGC 7 cut(s) 20, 23, 376, 1292, 1580, 1996, 2655
Sau96I GGNCC 7 cut(s) 262, 505, 530, 767, 984, 1595, 1705
ScaI AGTACT 1 cut(s) 2399
SchI GAGTC 7 cut(s) 188, 721, 2172, 2353, 2401, 2457, 2590
ScrFI CCNGG 7 cut(s) 80, 509, 561, 1178, 1281, 1282, 1434
SduI GDGCHC 6 cut(s) 976, 1187, 1658, 1822, 1874, 2782
SfaNI GCATC 4 cut(s) 1017, 1944, 2082, 2750
SfcI CTRYAG 2 cut(s) 2655, 2705
SfoI GGCGCC 1 cut(s) 1794
Sfr274I CTCGAG 1 cut(s) 196
SfuI TTCGAA 3 cut(s) 1329, 2076, 2584
SinI GGWCC 5 cut(s) 505, 530, 767, 984, 1595
SlaI CTCGAG 1 cut(s) 196
SmaI CCCGGG 1 cut(s) 1282
SmiMI CAYNNNNRTG 3 cut(s) 1412, 2046, 2253
SmlI CTYRAG 4 cut(s) 196, 731, 1862, 2000
SmoI CTYRAG 4 cut(s) 196, 731, 1862, 2000
SspDI GGCGCC 1 cut(s) 1792
SspI AATATT 2 cut(s) 714, 2272
SspMI CTAG 5 cut(s) 48, 365, 1397, 2475, 2627
StyD4I CCNGG 7 cut(s) 78, 507, 559, 1176, 1279, 1280, 1432
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 3 cut(s) 988, 1827, 1892
TaiI ACGT 2 cut(s) 234, 1750
TaqII GACCGA 2 cut(s) 2435, 2589
TatI WGTACW 1 cut(s) 2397
TauI GCSGC 2 cut(s) 1582, 1998
TfiI GAWTC 7 cut(s) 409, 687, 1381, 2067, 2184, 2233, 2648
TscAI CASTG 5 cut(s) 388, 697, 1446, 1663, 2382
TseFI GTSAC 2 cut(s) 181, 202
TseI GCWGC 5 cut(s) 19, 22, 375, 1291, 2654
Tsp45I GTSAC 2 cut(s) 181, 202
TspDTI ATGAA 7 cut(s) 44, 282, 1158, 1911, 2027, 2298, 2735
TspGWI ACGGA 2 cut(s) 147, 2771
TspMI CCCGGG 1 cut(s) 1280
TspRI CASTG 5 cut(s) 388, 697, 1446, 1663, 2382
Vha464I CTTAAG 1 cut(s) 1862
VneI GTGCAC 3 cut(s) 1183, 1818, 1870
VpaK11BI GGWCC 5 cut(s) 505, 530, 767, 984, 1595
VspI ATTAAT 1 cut(s) 1160
XagI CCTNNNNNAGG 2 cut(s) 1329, 1360
XapI RAATTY 3 cut(s) 814, 865, 1722
XceI RCATGY 2 cut(s) 1278, 2354
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XhoI CTCGAG 1 cut(s) 196
XmaI CCCGGG 1 cut(s) 1280
XmiI GTMKAC 2 cut(s) 882, 2436
XmnI GAANNNNTTC 3 cut(s) 449, 1001, 2700
XspI CTAG 5 cut(s) 48, 365, 1397, 2475, 2627
ZrmI AGTACT 1 cut(s) 2399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.