RLG00000025098

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003096
Physical Location & Seq
Reverse (-)
41956663 .. 41961989
5327 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025098

Sequence Viewer

Length: 2862 bp
ATGGCAACCATGGGGTTGCTGCTGTTTCTGGCTTTGTGCTCTGGGATTCAAGTGGTGTTCTCCGATGACGCTGCTGGGCTTCAATCCTTAAAGGCTGCATGGCGAAATTATCCACCCAGCTGGGACGGGTCAAGTGATTACTGTGGGTGGGAAGGAATCACCTGCAATCAGGAATCCAGTGTGACTGCAATGAGATTATCAGGCATGGGCCTGGTAGGGGATCTTAGTGGCGACATTGGGGCATTTACTGAATTAATAACCTTGGACCTTTCATTCAACCCTGGACTTACTGGTTCTCTCTCTTCACAATTAGGGCAGCTGCAAAAGTTAAATATTCTGATCCTAGCTGGCTGCAAATTCACTGGCAATATTCCAGATGAATTGGGAAATCTTGGAGAGCTAACCTTCTTGGCTCTGAATACAAACAATTTGACTGGTAAAATACCTGCTTCTTTGGGTAAACTCTCCAAACTCTACTGGCTGGACCTTGCAGAAAATCAGTTGACTGGACCTATCCCAGTTTCATCCATGACATCCCCAGGCTTGGACCAACTAGTGAAAGCTAAACATTTCCATTTCAACAAGAACCAGCTTTCAGGTTCAATTCCAGCCACACTATTCAGCGCTAAGATGGCCCTGATACATGTGTTGTTTGATGGAAACAAATTAAGTGGGACTATTCCATCAACAATAGGACAAGTTCAGACTCTTGAGGCTCTTCGGCTTGATAGAAATGCTCTGACGGGACCAGTCCCAAAAAGTATTAACAACCTTACAAATGTCAATGAATTAAATTTGGCCTTCAACAATTTGACAGGCCCTTTGCCCGACCTGACTGGAATGAACTCCCTCAATTATGTAGACCTTAGCAACAACTCATTTGACCCATCAGAAGCACCAAGTTGGTTCTCAGCTTTACCCGTACTCACCACTCTGGTTGTAGAATATGGACCACTTCAGGGTACTGTGCCAGAGAAGCTTTTCAGCCTACAAGATCTACAGCAAGTGAAACTGAAAAATAATGCATTTAACGACACACTGAACTTGGGTGATAGCATCAGTGCACAACTAGATGTTGTTGATCTGCAGAACAATGATATTTCCCAAGTCACTATAGGTTATGAGTACAAAAATTCATTAATACTTGTAGGGAACCCTGTGTGCAACACAACCACTGGTCTCCCTTATTGTCAGATTCCGCAAACTACAACATATTCTACTACCGTTAGTTGTGGAAACGCTAAATGCCCCGAGGATCAGAAGCTCAGCCCTCAGAGTTGTCAGTGTGCATATCCGTTTGAAGGAACACTGTACTTTAGAGCACCCACTTTCAGGGATTTATCAGATGCAAATAAGTTTCACTCCCTAGAAATGAGCCTGTGGTCGAAACTTGGCCTCAATCCTGGTTCAGTTGCTCTTGAGAACCCTTTCTTCAACATTGATGACTATCTTCAAATACAGTTGGCACTCTTTCCATCTATGGGAACCTATTTTAATAGGTCAGAGATTCTGAGGATTGGTTTTGATCTGAGCAATCAAACTTACAAGCCCCCCGCTGAGTTTGGTCCCTATATTTTTATTCCAGCTCCTTATACTTTCCGGGCCGCGCATAAGAAATCTGTAAGCACTGGTGTTGTCATTGGGATAGCAATTGGTTGTACCTTTCTGGTTTTGGGCTTAGTGGGAGTCGGAATATATGCCATTCGGCAAAAAAAACGTGCAGAAAGAGCCATTGGATTAAGCAGACCATTTGCTTCTTGGGCACCCAGCGGAAAAGATAGTGGGGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCTTATGAGGAGCTTAAGAAGTGCACCAATAATTTCTCTGAAAATAATGAGATAGGATCCGGCGGCTACGGCAAGGTTTACAGGGGCATGTTTGCTGATGGACTAGTTGTGGCAATCAAAAGAGCTCAGCAAGGATCAATGCAGGGTGGTCTCGAGTTCAAGACTGAAATTGAATTGCTCTCACGAGTTCATCACAAAAATCTTGTCGGTCTTGTCGGGTTTTGTTTTGAACAAGGAGAACAGATGCTGGTTTACGAGTATATGCCTAATGGAACACTTAGGGAAAGCTTGTCAGGGAGATCTGGCATTCATCTCGACTGGAAGAGAAGACTCCGCATCACTCTGGGCTCAGCAAGAGGACTAGCATACCTACATGAGCTTGCCAACCCTCCTATAATCCATAGAGATATTAAGACCACCAATATTCTATTGGATGAACATTTAACGGCAAAGGTTGCAGATTTTGGCTTGTCCAAGCTGGTTTCTGACAGTGCAAAAGGGCATGTTTCAACTCAGGTCAAAGGCACACTGGGATATCTGGATCCTGAATACTACATGACTCAACAGTTAACTGAGAAGAGTGATGTATACAGTTTTGGAGTGGTTATGCTTGAATTGATAACTGCAAAGCAACCGATAGAGAAGGGAAAATACATTGTCCGCGAGGTACGCATGATAATGAACAAGGATGATGAAGAGTACTACGGGTTGAGGGAGTTAATAGATCGAAACATTAGAAACTCAGGACCCCTTATAGGTTTTGCAAGGTTCTTGGAGTTGGCCATGCAATGCGTTGAAGAATCAGCTGCAGATCGTCCAACAATGAGTGATCTTGTCAAGGCTGTTGAAACCATTCTACAGAATGATGGGATGAATACAAACTCAACATCAGCCTCCTCATCTGCCACTGACTTTAATTCTTCAAAAAGTGCTCCTAAACATCCATACAACGACGGTTTGGCAAAGAAGGATGTGACTGACACTAATAGCGCCTTTGAATACAGTGGCGGATACACCGTTTCTGCGAAAGTAGAACCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

104.03

Weight (kDa)

5.84

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 56 2.3e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 236 - 293 5.9e-06 Leucine rich repeat
Pkinase PF00069 619 - 886 5.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 621 - 886 6.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 2 cut(s) 170, 454
AccB1I GGYRCC 1 cut(s) 1762
AccI GTMKAC 2 cut(s) 861, 2406
AccII CGCG 2 cut(s) 1607, 2481
AciI CCGC 8 cut(s) 1199, 1554, 1605, 1770, 1881, 2152, 2479, 2826
AclWI GGATC 8 cut(s) 228, 334, 1263, 1869, 1882, 1960, 2354, 2367
AcoI YGGCCR 1 cut(s) 2598
AcsI RAATTY 3 cut(s) 356, 793, 1132
AcuI CTGAAG 1 cut(s) 941
AfaI GTAC 7 cut(s) 924, 964, 1127, 1313, 1660, 2487, 2519
AfeI AGCGCT 1 cut(s) 625
AfiI CCNNNNNNNGG 7 cut(s) 217, 544, 959, 1301, 1332, 1481, 2573
AflII CTTAAG 1 cut(s) 1832
AflIII ACRYGT 1 cut(s) 643
AhlI ACTAGT 2 cut(s) 553, 1921
AjnI CCWGG 4 cut(s) 210, 280, 538, 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 1716, 1748
Alw21I GWGCWC 7 cut(s) 41, 1066, 1324, 1792, 1844, 1945, 2752
Alw26I GTCTC 2 cut(s) 1184, 1973
Alw44I GTGCAC 3 cut(s) 1062, 1788, 1840
AlwI GGATC 8 cut(s) 228, 334, 1263, 1869, 1882, 1960, 2354, 2367
AlwNI CAGNNNCTG 1 cut(s) 2065
Ama87I CYCGRG 2 cut(s) 1250, 1970
Aor51HI AGCGCT 1 cut(s) 625
AoxI GGCC 7 cut(s) 208, 633, 798, 817, 1393, 1602, 2598
ApaLI GTGCAC 3 cut(s) 1062, 1788, 1840
ApeKI GCWGC 7 cut(s) 19, 71, 95, 316, 319, 351, 2624
ApoI RAATTY 3 cut(s) 356, 793, 1132
ArsI GACNNNNNNTTYG 4 cut(s) 805, 837, 2007, 2039
AseI ATTAAT 2 cut(s) 254, 1139
Asp700I GAANNNNTTC 3 cut(s) 980, 1427, 2670
AspLEI GCGC 3 cut(s) 626, 1609, 2810
AsuC2I CCSGG 1 cut(s) 1601
AsuHPI GGTGA 3 cut(s) 151, 919, 1061
AvaI CYCGRG 2 cut(s) 1250, 1970
AvaII GGWCC 8 cut(s) 265, 484, 509, 547, 746, 950, 1565, 2564
BaeGI GKGCMC 4 cut(s) 1066, 1765, 1792, 1844
BaeI ACNNNNGTAYC 2 cut(s) 632, 665
BalI TGGCCA 1 cut(s) 2600
BamHI GGATCC 2 cut(s) 1874, 2359
BanI GGYRCC 1 cut(s) 1762
BanII GRGCYC 2 cut(s) 1945, 2168
BarI GAAGNNNNNNTAC 2 cut(s) 2453, 2485
BauI CACGAG 1 cut(s) 2001
BbsI GAAGAC 1 cut(s) 2152
Bbv12I GWGCWC 7 cut(s) 41, 1066, 1324, 1792, 1844, 1945, 2752
BbvI GCAGC 7 cut(s) 6, 58, 82, 306, 328, 338, 2611
BccI CCATC 8 cut(s) 625, 650, 691, 895, 1483, 1806, 1910, 2678
BceAI ACGGC 2 cut(s) 1903, 2280
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 2113, 2147
BciT130I CCWGG 4 cut(s) 212, 282, 540, 1404
BciVI GTATCC 1 cut(s) 2822
BcnI CCSGG 1 cut(s) 1601
BcoDI GTCTC 2 cut(s) 1184, 1973
BcuI ACTAGT 2 cut(s) 553, 1921
BfaI CTAG 6 cut(s) 344, 554, 1070, 1367, 1922, 2180
BfmI CTRYAG 5 cut(s) 998, 1085, 1113, 2625, 2675
BfoI RGCGCY 2 cut(s) 627, 2811
BfrI CTTAAG 1 cut(s) 1832
BfuAI ACCTGC 2 cut(s) 170, 454
BfuI GTATCC 1 cut(s) 2822
BglII AGATCT 2 cut(s) 994, 2117
BisI GCNGC 9 cut(s) 20, 72, 96, 317, 320, 352, 1605, 1882, 2625
BlpI GCTNAGC 3 cut(s) 1265, 1944, 2167
BlsI GCNGC 9 cut(s) 21, 73, 97, 318, 321, 353, 1606, 1883, 2626
BmcAI AGTACT 1 cut(s) 2519
Bme1390I CCNGG 5 cut(s) 212, 282, 540, 1404, 1601
Bme18I GGWCC 8 cut(s) 265, 484, 509, 547, 746, 950, 1565, 2564
BmeT110I CYCGRG 2 cut(s) 1250, 1970
BmiI GGNNCC 8 cut(s) 747, 1154, 1486, 1567, 1764, 1876, 2361, 2566
BmrFI CCNGG 5 cut(s) 212, 282, 540, 1404, 1601
BmrI ACTGGG 2 cut(s) 512, 2357
BmsI GCATC 4 cut(s) 1065, 1336, 2052, 2163
BmuI ACTGGG 2 cut(s) 512, 2357
BpiI GAAGAC 1 cut(s) 2152
Bpu10I CCTNAGC 1 cut(s) 866
Bpu1102I GCTNAGC 3 cut(s) 1265, 1944, 2167
BpuEI CTTGAG 2 cut(s) 731, 1439
BpuMI CCSGG 1 cut(s) 1601
BsaBI GATNNNNATC 1 cut(s) 1446
BsaI GGTCTC 2 cut(s) 1184, 1973
BsaJI CCNNGG 5 cut(s) 9, 261, 280, 538, 1251
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 544, 959, 1301, 1332, 1481, 2573
Bse3DI GCAATG 2 cut(s) 195, 2612
Bse8I GATNNNNATC 1 cut(s) 1446
BseBI CCWGG 4 cut(s) 212, 282, 540, 1404
BseDI CCNNGG 5 cut(s) 9, 261, 280, 538, 1251
BseGI GGATG 7 cut(s) 524, 533, 2257, 2512, 2694, 2758, 2794
BseJI GATNNNNATC 1 cut(s) 1446
BseLI CCNNNNNNNGG 7 cut(s) 217, 544, 959, 1301, 1332, 1481, 2573
BseMI GCAATG 2 cut(s) 195, 2612
BseRI GAGGAG 2 cut(s) 1841, 2704
BseSI GKGCMC 4 cut(s) 1066, 1765, 1792, 1844
BseXI GCAGC 7 cut(s) 6, 58, 82, 306, 328, 338, 2611
BseYI CCCAGC 4 cut(s) 74, 116, 120, 1766
BsgI GTGCAG 1 cut(s) 1740
Bsh1236I CGCG 2 cut(s) 1607, 2481
BshFI GGCC 7 cut(s) 210, 635, 800, 819, 1395, 1604, 2600
BshNI GGYRCC 1 cut(s) 1762
BsiHKAI GWGCWC 7 cut(s) 41, 1066, 1324, 1792, 1844, 1945, 2752
BsiHKCI CYCGRG 2 cut(s) 1250, 1970
BsiSI CCGG 2 cut(s) 1600, 1878
BslFI GGGAC 5 cut(s) 137, 688, 737, 759, 1551
BslI CCNNNNNNNGG 7 cut(s) 217, 544, 959, 1301, 1332, 1481, 2573
BsmAI GTCTC 2 cut(s) 1184, 1973
BsmFI GGGAC 5 cut(s) 137, 688, 737, 759, 1551
BsmI GAATGC 1 cut(s) 2124
BsnI GGCC 7 cut(s) 210, 635, 800, 819, 1395, 1604, 2600
Bso31I GGTCTC 2 cut(s) 1184, 1973
BsoBI CYCGRG 2 cut(s) 1250, 1970
Bsp1286I GDGCHC 9 cut(s) 41, 1066, 1324, 1765, 1792, 1844, 1945, 2168, 2752
Bsp1720I GCTNAGC 3 cut(s) 1265, 1944, 2167
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 8 cut(s) 1199, 1554, 1605, 1770, 1881, 2152, 2479, 2826
BspANI GGCC 7 cut(s) 210, 635, 800, 819, 1395, 1604, 2600
BspFNI CGCG 2 cut(s) 1607, 2481
BspLI GGNNCC 8 cut(s) 747, 1154, 1486, 1567, 1764, 1876, 2361, 2566
BspMAI CTGCAG 2 cut(s) 1089, 2629
BspMI ACCTGC 2 cut(s) 170, 454
BspPI GGATC 8 cut(s) 228, 334, 1263, 1869, 1882, 1960, 2354, 2367
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 1762
BspTI CTTAAG 1 cut(s) 1832
BspTNI GGTCTC 2 cut(s) 1184, 1973
BsrDI GCAATG 2 cut(s) 195, 2612
BssECI CCNNGG 5 cut(s) 9, 261, 280, 538, 1251
BssNAI GTATAC 1 cut(s) 2407
BssSI CACGAG 1 cut(s) 2001
BssT1I CCWWGG 2 cut(s) 9, 261
Bst1107I GTATAC 1 cut(s) 2407
Bst2BI CACGAG 1 cut(s) 2001
Bst2UI CCWGG 4 cut(s) 212, 282, 540, 1404
Bst6I CTCTTC 5 cut(s) 307, 723, 2135, 2390, 2508
BstAFI CTTAAG 1 cut(s) 1832
BstAPI GCANNNNNTGC 1 cut(s) 2273
BstC8I GCNNGC 2 cut(s) 349, 2199
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 7 cut(s) 524, 533, 2257, 2512, 2694, 2758, 2794
BstFNI CGCG 2 cut(s) 1607, 2481
BstH2I RGCGCY 2 cut(s) 627, 2811
BstHHI GCGC 3 cut(s) 626, 1609, 2810
BstMAI GTCTC 2 cut(s) 1184, 1973
BstMWI GCNNNNNNNGC 7 cut(s) 632, 976, 1727, 1760, 1887, 2273, 2487
BstNI CCWGG 4 cut(s) 212, 282, 540, 1404
BstNSI RCATGY 3 cut(s) 647, 1909, 2324
BstSCI CCNGG 5 cut(s) 210, 280, 538, 1402, 1599
BstSFI CTRYAG 5 cut(s) 998, 1085, 1113, 2625, 2675
BstSLI GKGCMC 4 cut(s) 1066, 1765, 1792, 1844
BstUI CGCG 2 cut(s) 1607, 2481
BstV1I GCAGC 7 cut(s) 6, 58, 82, 306, 328, 338, 2611
BstV2I GAAGAC 1 cut(s) 2152
BstX2I RGATCY 5 cut(s) 220, 994, 1874, 2117, 2359
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 5 cut(s) 220, 994, 1874, 2117, 2359
BstZ17I GTATAC 1 cut(s) 2407
BsuI GTATCC 1 cut(s) 2822
BsuRI GGCC 7 cut(s) 210, 635, 800, 819, 1395, 1604, 2600
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 7 cut(s) 524, 533, 2257, 2512, 2694, 2758, 2794
BveI ACCTGC 2 cut(s) 170, 454
Cac8I GCNNGC 2 cut(s) 349, 2199
CaiI CAGNNNCTG 1 cut(s) 2065
CfoI GCGC 3 cut(s) 626, 1609, 2810
CseI GACGC 1 cut(s) 77
Csp6I GTAC 7 cut(s) 923, 963, 1126, 1312, 1659, 2486, 2518
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 919, 954
CviQI GTAC 7 cut(s) 923, 963, 1126, 1312, 1659, 2486, 2518
EaeI YGGCCR 1 cut(s) 2598
Eam1104I CTCTTC 5 cut(s) 307, 723, 2135, 2390, 2508
EarI CTCTTC 5 cut(s) 307, 723, 2135, 2390, 2508
EciI GGCGGA 1 cut(s) 2841
Ecl136II GAGCTC 1 cut(s) 1943
Eco130I CCWWGG 2 cut(s) 9, 261
Eco24I GRGCYC 2 cut(s) 1945, 2168
Eco31I GGTCTC 2 cut(s) 1184, 1973
Eco32I GATATC 1 cut(s) 2354
Eco47I GGWCC 8 cut(s) 265, 484, 509, 547, 746, 950, 1565, 2564
Eco47III AGCGCT 1 cut(s) 625
Eco53kI GAGCTC 1 cut(s) 1943
Eco57I CTGAAG 1 cut(s) 941
Eco88I CYCGRG 2 cut(s) 1250, 1970
EcoICRI GAGCTC 1 cut(s) 1943
EcoO109I RGGNCCY 2 cut(s) 818, 2564
EcoRII CCWGG 4 cut(s) 210, 280, 538, 1402
EcoRV GATATC 1 cut(s) 2354
EcoT14I CCWWGG 2 cut(s) 9, 261
EcoT22I ATGCAT 1 cut(s) 1027
EcoT38I GRGCYC 2 cut(s) 1945, 2168
ErhI CCWWGG 2 cut(s) 9, 261
FaqI GGGAC 5 cut(s) 137, 688, 737, 759, 1551
FauI CCCGC 1 cut(s) 1561
FblI GTMKAC 2 cut(s) 861, 2406
Fnu4HI GCNGC 9 cut(s) 20, 72, 96, 317, 320, 352, 1605, 1882, 2625
FokI GGATG 7 cut(s) 511, 520, 2264, 2519, 2701, 2745, 2801
FriOI GRGCYC 2 cut(s) 1945, 2168
Fsp4HI GCNGC 9 cut(s) 20, 72, 96, 317, 320, 352, 1605, 1882, 2625
FspBI CTAG 6 cut(s) 344, 554, 1070, 1367, 1922, 2180
GlaI GCGC 3 cut(s) 625, 1608, 2809
GluI GCNGC 9 cut(s) 20, 72, 96, 317, 320, 352, 1605, 1882, 2625
GsaI CCCAGC 4 cut(s) 78, 120, 124, 1770
HaeII RGCGCY 2 cut(s) 627, 2811
HaeIII GGCC 7 cut(s) 210, 635, 800, 819, 1395, 1604, 2600
HapII CCGG 2 cut(s) 1600, 1878
HgaI GACGC 1 cut(s) 77
HhaI GCGC 3 cut(s) 626, 1609, 2810
Hin6I GCGC 3 cut(s) 624, 1607, 2808
HinP1I GCGC 3 cut(s) 624, 1607, 2808
HincII GTYRAC 2 cut(s) 504, 2388
HindII GTYRAC 2 cut(s) 504, 2388
HindIII AAGCTT 2 cut(s) 977, 2104
HpaI GTTAAC 1 cut(s) 2388
HpaII CCGG 2 cut(s) 1600, 1878
HphI GGTGA 3 cut(s) 151, 919, 1061
Hpy99I CGWCG 1 cut(s) 2774
HpyAV CCTTC 6 cut(s) 146, 415, 811, 1295, 2455, 2779
HpyCH4IV ACGT 1 cut(s) 1717
HpyF10VI GCNNNNNNNGC 7 cut(s) 632, 976, 1727, 1760, 1887, 2273, 2487
HpySE526I ACGT 1 cut(s) 1717
HspAI GCGC 3 cut(s) 624, 1607, 2808
KspAI GTTAAC 1 cut(s) 2388
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 4 cut(s) 1591, 1805, 1828, 2755
Lsp1109I GCAGC 7 cut(s) 6, 58, 82, 306, 328, 338, 2611
LweI GCATC 4 cut(s) 1065, 1336, 2052, 2163
MaeI CTAG 6 cut(s) 344, 554, 1070, 1367, 1922, 2180
MaeII ACGT 1 cut(s) 1717
MaeIII GTNAC 3 cut(s) 181, 1108, 2791
MfeI CAATTG 1 cut(s) 1650
MflI RGATCY 5 cut(s) 220, 994, 1874, 2117, 2359
MhlI GDGCHC 9 cut(s) 41, 1066, 1324, 1765, 1792, 1844, 1945, 2168, 2752
MlsI TGGCCA 1 cut(s) 2600
MluNI TGGCCA 1 cut(s) 2600
MlyI GAGTC 4 cut(s) 700, 1695, 2142, 2371
MmeI TCCRAC 2 cut(s) 1669, 2660
Mox20I TGGCCA 1 cut(s) 2600
Mph1103I ATGCAT 1 cut(s) 1027
MroXI GAANNNNTTC 3 cut(s) 980, 1427, 2670
MscI TGGCCA 1 cut(s) 2600
MslI CAYNNNNRTG 1 cut(s) 2495
Msp20I TGGCCA 1 cut(s) 2600
MspA1I CMGCKG 5 cut(s) 120, 319, 1556, 1770, 2624
MspCI CTTAAG 1 cut(s) 1832
MspI CCGG 2 cut(s) 1600, 1878
MspR9I CCNGG 5 cut(s) 212, 282, 540, 1404, 1601
MunI CAATTG 1 cut(s) 1650
Mva1269I GAATGC 1 cut(s) 2124
MvaI CCWGG 4 cut(s) 212, 282, 540, 1404
MvnI CGCG 2 cut(s) 1607, 2481
MwoI GCNNNNNNNGC 7 cut(s) 632, 976, 1727, 1760, 1887, 2273, 2487
NciI CCSGG 1 cut(s) 1601
NcoI CCATGG 1 cut(s) 9
NlaIV GGNNCC 8 cut(s) 747, 1154, 1486, 1567, 1764, 1876, 2361, 2566
NmuCI GTSAC 3 cut(s) 181, 1108, 2791
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 3 cut(s) 647, 1909, 2324
PaeR7I CTCGAG 1 cut(s) 1970
PaqCI CACCTGC 1 cut(s) 170
PciI ACATGT 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 2124
PdmI GAANNNNTTC 3 cut(s) 980, 1427, 2670
PfeI GAWTC 6 cut(s) 46, 156, 173, 1195, 1507, 2618
PkrI GCNGC 9 cut(s) 21, 73, 97, 318, 321, 353, 1606, 1883, 2626
PleI GAGTC 4 cut(s) 700, 1694, 2142, 2371
PpsI GAGTC 4 cut(s) 700, 1694, 2142, 2371
PpuMI RGGWCCY 1 cut(s) 2564
PscI ACATGT 1 cut(s) 643
PshBI ATTAAT 2 cut(s) 254, 1139
Psp124BI GAGCTC 1 cut(s) 1945
Psp5II RGGWCCY 1 cut(s) 2564
Psp6I CCWGG 4 cut(s) 210, 280, 538, 1402
PspFI CCCAGC 4 cut(s) 74, 116, 120, 1766
PspGI CCWGG 4 cut(s) 210, 280, 538, 1402
PspN4I GGNNCC 8 cut(s) 747, 1154, 1486, 1567, 1764, 1876, 2361, 2566
PspPPI RGGWCCY 1 cut(s) 2564
PstI CTGCAG 2 cut(s) 1089, 2629
PstNI CAGNNNCTG 1 cut(s) 2065
PsuI RGATCY 5 cut(s) 220, 994, 1874, 2117, 2359
PvuII CAGCTG 3 cut(s) 120, 319, 2624
RsaI GTAC 7 cut(s) 924, 964, 1127, 1313, 1660, 2487, 2519
RsaNI GTAC 7 cut(s) 923, 963, 1126, 1312, 1659, 2486, 2518
RseI CAYNNNNRTG 1 cut(s) 2495
SacI GAGCTC 1 cut(s) 1945
SapI GCTCTTC 1 cut(s) 723
SatI GCNGC 9 cut(s) 20, 72, 96, 317, 320, 352, 1605, 1882, 2625
ScaI AGTACT 1 cut(s) 2519
SchI GAGTC 4 cut(s) 700, 1695, 2142, 2371
ScrFI CCNGG 5 cut(s) 212, 282, 540, 1404, 1601
SduI GDGCHC 9 cut(s) 41, 1066, 1324, 1765, 1792, 1844, 1945, 2168, 2752
SfaNI GCATC 4 cut(s) 1065, 1336, 2052, 2163
SfcI CTRYAG 5 cut(s) 998, 1085, 1113, 2625, 2675
Sfr274I CTCGAG 1 cut(s) 1970
SinI GGWCC 8 cut(s) 265, 484, 509, 547, 746, 950, 1565, 2564
SlaI CTCGAG 1 cut(s) 1970
SmiMI CAYNNNNRTG 1 cut(s) 2495
SmlI CTYRAG 4 cut(s) 710, 1418, 1832, 1970
SmoI CTYRAG 4 cut(s) 710, 1418, 1832, 1970
SpeI ACTAGT 2 cut(s) 553, 1921
SsiI CCGC 8 cut(s) 1199, 1554, 1605, 1770, 1881, 2152, 2479, 2826
SspI AATATT 3 cut(s) 334, 370, 2242
SspMI CTAG 6 cut(s) 344, 554, 1070, 1367, 1922, 2180
SstI GAGCTC 1 cut(s) 1945
StyD4I CCNGG 5 cut(s) 210, 280, 538, 1402, 1599
StyI CCWWGG 2 cut(s) 9, 261
TaiI ACGT 1 cut(s) 1720
TaqI TCGA 4 cut(s) 1385, 1971, 2133, 2545
TaqII GACCGA 1 cut(s) 2015
TatI WGTACW 3 cut(s) 1125, 1311, 2517
TauI GCSGC 2 cut(s) 1607, 1884
TfiI GAWTC 6 cut(s) 46, 156, 173, 1195, 1507, 2618
TseFI GTSAC 3 cut(s) 181, 1108, 2791
TseI GCWGC 7 cut(s) 19, 71, 95, 316, 319, 351, 2624
Tsp45I GTSAC 3 cut(s) 181, 1108, 2791
TspGWI ACGGA 1 cut(s) 1284
Vha464I CTTAAG 1 cut(s) 1832
VneI GTGCAC 3 cut(s) 1062, 1788, 1840
VpaK11BI GGWCC 8 cut(s) 265, 484, 509, 547, 746, 950, 1565, 2564
VspI ATTAAT 2 cut(s) 254, 1139
XapI RAATTY 3 cut(s) 356, 793, 1132
XceI RCATGY 3 cut(s) 647, 1909, 2324
XcmI CCANNNNNNNNNTGG 2 cut(s) 1755, 2245
XhoI CTCGAG 1 cut(s) 1970
XmiI GTMKAC 2 cut(s) 861, 2406
XmnI GAANNNNTTC 3 cut(s) 980, 1427, 2670
XspI CTAG 6 cut(s) 344, 554, 1070, 1367, 1922, 2180
ZrmI AGTACT 1 cut(s) 2519
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.