Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003096

Physical Location & Seq

Genomic Coordinates Reverse (-)

27314013 .. 27319578

Gene Structure 5566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1182200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGCGGCTGCTGCTGTTCTTTCTGGCTTTCTGCTTCTCTGGAATTCATGTGATATCTTCTTCTACACACATAGATGATGCTAGTGCACTCCAGTCCTTGAGGCAGACATGGAGCAACGTTCCACCCAGCTGGGACAAGTCAAACGATCCATGTGGGGAGCGCTGGGAAGGCATCACTTGCAACAGTTCGAGGGTGACTGCACTGCGATTATCAGGAATGAACGTGCAAGGGGAAATTGAAGGCGACATCGCGGGGCTCTCTGAGTTAAGATCCTTGGATCTTTCATTCAACAAAGGCCTCACCGGTTCTCTCTCTCCACGATTAGGGGATCTGAGCAATCTAAACTTCCTGATCCTAGCTGGTTGCGGCTTCAGTGGAATTATTCCAAGTGAATTGGGGAAACTTGGAGAGCTACTCTTCTTGGCTTTGAATACGAATAACTTCACCGGTAGTATACCTGCTTCTTTGGGTAATCTCTCCAACCTCTATTGGCTGGACCTGGCAGACAATCAGTTGACTGGACCTCTCCCAGTTTCGAGCGGCACTACCCCCGGCTTAGACCAACTGTTTAACGCTAAACATTTCCATTTGAACCAGAACCAGCTTTCAGGTACCATTCCACCCAGACTTTTCAGCTCCAAGATGACACTGATCCATGTATTGTTTGATGGAAATGGATTCACTGGGACTATTCCATCAACAATATTTTCCGTTCAGACTCTCGACATTCTAGACCTTAGTTACAACTCGTTTGATCCGTCAGAAGCTCCGGGTTGGTTCTCAACCTTGCCATCGCTCACCACTATCGTTTTAGAAAATGTAGCACTTCAAGGGACTGTGCCAGAGCAGATGTTCAGCATACAGTCATTGCAGCAAGTGAAACTGAAAAACAATGCGTTTAACGACACATTTAACATGGGTGATTACATCAGTCCGCAACTGACGCTTGTTGATCTGCAGAACAATCAGATTCAGAGAATAACCCAGAGTTATAAGTACAAAAATACATTAATACTTGTTGGGAACCCATTGTGCAATGAATCTAGCTCGAGTCCGTACTGTCAGCCTCCACAATCAAATACAAAACCGTATGCTACTAGCACGGATTGTCCATCCATTACATGCCCCGGGGGTCAGCAGCCAAGCCCTCAGAGTTGTCATTGTGGATATCCTTTTGAAGGAACACTGGATTTCCGAGCGCCCACTTTCAGGGAATTGACAAATGTGACCCTGTTTCAATCCCTAGAACAATGGGTGAAACTTCACCTCACTCCCGGTTCAGTGTCACTTGATAACCCTTTCTTCGACAACGATGACCATCTTAAAGTACATGTGGCTCTCTTTCCACCTACAGGAACATATTTTAATAGGTCTGAGATTATCAGGATTGGTTTTCTTATGAGCAGTCAGCCTTACATGCCGCCAGAAGGGTTTGGACCCTTCGTTTTTATTCCAGCTCAATATAATTTCCCAGACACACATAAAAGCTTTATGAGCACTGGTGTTATTGTTGGGATATCAGTTAGTTGTCTCGTTCTTGTTGTGGGCCTCGTGGTAGTAGGAATTTACGCCATTAGGCAAAGGAAACGTGCGGAAAGAGCCATTGGTTTAAGCAGACCTTTCGCTTCTTGGGTGCCAAGCGGAAATGATAGTGGCGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCGTATGATGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAGTAATGAGATAGGATCTGGCGGCTACGGGAAGGTTTACAGGGGCATGCTTTCTGATGGACAAGTGATAGCAATCAAAAGAGCTCGGCAAGGATCAATGCAGGGAGGCGTCGAGTTCACGACTGAAATTGAGTTGCTCTCTCGCGTTCATCACAAAAATGTTGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTATATGCCTAATGGAACACTTAGGGAAAGCTTGTCAGGGAGATCTGGTATTCATCTCAATTGGAAGAGGAGACTCCAAATCACTCTTGGCTCGGCAAGAGGACTGGCTTACCTACATGAACTTGCAAATCCTCCTATAATTCACAGAGATGTGAAGTCCACCAATATTCTATTAGATGAACATTTAACAGCGAAGGTTGCAGATTTTGGCCTGTCGATGCTGGTCAATGACAGCGGAGAAGAACATGTCTCGACTCATGTCAAAGGAACACTAGGCTATCTGGATCCTGAGTATTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCAGCCAATTGAAAAGGGAAAATACATTGTCCGCGAGGTACGATTGGTGATGAACAAGAATGACGAAGAGCATTACGGTTTGAGGGAGTTAATAGATCGAAACATTCGAAACTCAGGAACTCTTATCGGGTTTGGGAGGTTCTTGGAGCTAGCCATGCAATGCGTCGAAGAATCAGCTGCAGATCGTCCCACGATGAGTGAAGTTGTGAGGGCCATCGAAACCATTCTGCAGAACGATGGGATGAACACAAACTCAACATCAGCATCTTCATCAGCCACGGAGTTTGCAGCGTCAAAAGGTGCTCCGAAACATCCCTACAACGTTGGCTTGCCGAAAAAGGAATTCAATGACAGCACCGGCTCCTTTGATTACAGCGGTGGATGCGCTGTTTCAGCGAAAATTGAACCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 Pkinase_Tyr

Protein Analysis

911

Amino Acids

100.0

Weight (kDa)

5.84

Isoelectric Point (pI)

45.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	25 - 61	1.8e-07	Leucine rich repeat N-terminal domain
Pkinase	PF00069	576 - 843	5.9e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	579 - 844	1.9e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003096)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79620 AT1G79620 AT1G79620 AT1G79620 AT1G79620
fragaria_vesca	FvH4_6g09020 FvH4_6g09020
malus_domestica	MD04G1181800.v1.1 MD04G1182200.v1.1 MD04G1183000.v1.1 MD04G1183100.v1.1 MD12G1196600.v1.1
prunus_persica	Prupe.6G301100_v2.0.a1 Prupe.6G301100_v2.0.a1 Prupe.6G301200_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1
pyrus_communis	pycom04g16070 pycom04g16150 pycom04g16170 pycom12g18280
rosa_chinensis	RchiOBHm_Chr3g0459271
rosa_laevigata	RLG00000025098
rosa_multiflora	Rmu_sc0006220.1_g000001 Rmu_sc0010702.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420350
rosa_rugosa	Rorug03G0034900 Rorug03G0035000
rosa_samantha	Rh3AG092700 Rh3BG095800 Rh3CG096800 Rh3DG096400
rosa_wichuraiana	Rw3G007970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	993
Acc36I	ACCTGC	1 cut(s)	466
Acc65I	GGTACC	1 cut(s)	611
AccB1I	GGYRCC	2 cut(s)	611, 1633
AccBSI	CCGCTC	1 cut(s)	540
AccI	GTMKAC	2 cut(s)	454, 2277
AccII	CGCG	3 cut(s)	251, 1875, 2352
AclI	AACGTT	2 cut(s)	117, 2640
AcsI	RAATTY	3 cut(s)	42, 1563, 2660
AcuI	CTGAAG	1 cut(s)	355
AcyI	GRCGYC	1 cut(s)	1839
AfaI	GTAC	5 cut(s)	613, 998, 1058, 1329, 2358
AfeI	AGCGCT	1 cut(s)	161
AfiI	CCNNNNNNNGG	5 cut(s)	323, 1178, 1209, 1352, 1427
AflII	CTTAAG	1 cut(s)	1703
AflIII	ACRYGT	2 cut(s)	1330, 2191
AgeI	ACCGGT	2 cut(s)	302, 446
AjnI	CCWGG	1 cut(s)	498
AjuI	GAANNNNNNNTTGG	2 cut(s)	1587, 1619
Alw21I	GWGCWC	6 cut(s)	88, 1499, 1663, 1715, 1816, 2623
Alw26I	GTCTC	3 cut(s)	1535, 2011, 2200
Alw44I	GTGCAC	3 cut(s)	84, 1659, 1711
AlwNI	CAGNNNCTG	1 cut(s)	1936
Ama87I	CYCGRG	2 cut(s)	1048, 1127
Aor51HI	AGCGCT	1 cut(s)	161
AoxI	GGCC	4 cut(s)	295, 1546, 2155, 2529
ApaLI	GTGCAC	3 cut(s)	84, 1659, 1711
ApeKI	GCWGC	7 cut(s)	7, 10, 871, 1138, 2320, 2495, 2606
ApoI	RAATTY	3 cut(s)	42, 1563, 2660
AseI	ATTAAT	1 cut(s)	1010
AsiGI	ACCGGT	2 cut(s)	302, 446
Asp700I	GAANNNNTTC	2 cut(s)	440, 2541
Asp718I	GGTACC	1 cut(s)	611
AspLEI	GCGC	3 cut(s)	162, 1201, 2705
AspS9I	GGNCC	5 cut(s)	496, 521, 1436, 1546, 2529
AsuC2I	CCSGG	5 cut(s)	552, 771, 1128, 1129, 1275
AsuHPI	GGTGA	8 cut(s)	205, 292, 436, 790, 932, 1256, 1267, 2377
AsuII	TTCGAA	2 cut(s)	1917, 2425
AsuNHI	GCTAGC	1 cut(s)	2467
AvaI	CYCGRG	2 cut(s)	1048, 1127
AvaII	GGWCC	3 cut(s)	496, 521, 1436
BaeGI	GKGCMC	3 cut(s)	88, 1663, 1715
BamHI	GGATCC	1 cut(s)	2230
BanI	GGYRCC	2 cut(s)	611, 1633
BanII	GRGCYC	2 cut(s)	258, 1816
BarI	GAAGNNNNNNTAC	2 cut(s)	445, 477
BauI	CACGAG	1 cut(s)	1550
Bbv12I	GWGCWC	6 cut(s)	88, 1499, 1663, 1715, 1816, 2623
BbvI	GCAGC	5 cut(s)	883, 1150, 2332, 2482, 2618
BccI	CCATC	9 cut(s)	662, 703, 799, 1120, 1326, 1677, 1781, 2540, 2549
BcgI	CGANNNNNNTGC	2 cut(s)	1603, 1637
BciT130I	CCWGG	1 cut(s)	500
BcnI	CCSGG	5 cut(s)	552, 771, 1128, 1129, 1275
BcoDI	GTCTC	3 cut(s)	1535, 2011, 2200
BfaI	CTAG	9 cut(s)	81, 356, 731, 1044, 1098, 1244, 2219, 2316, 2468
BfmI	CTRYAG	4 cut(s)	956, 1350, 2496, 2546
BfoI	RGCGCY	2 cut(s)	163, 1202
BfrI	CTTAAG	1 cut(s)	1703
BfuAI	ACCTGC	1 cut(s)	466
BglII	AGATCT	1 cut(s)	1988
Bme1390I	CCNGG	6 cut(s)	500, 552, 771, 1128, 1129, 1275
Bme18I	GGWCC	3 cut(s)	496, 521, 1436
BmeT110I	CYCGRG	2 cut(s)	1048, 1127
BmgT120I	GGNCC	5 cut(s)	496, 521, 1436, 1546, 2529
BmiI	GGNNCC	6 cut(s)	613, 1025, 1438, 1635, 2232, 2680
BmrFI	CCNGG	6 cut(s)	500, 552, 771, 1128, 1129, 1275
BmrI	ACTGGG	2 cut(s)	524, 693
BmsI	GCATC	6 cut(s)	67, 180, 1923, 2154, 2591, 2690
BmtI	GCTAGC	1 cut(s)	2471
BmuI	ACTGGG	2 cut(s)	524, 693
BoxI	GACNNNNGTC	1 cut(s)	2204
BplI	GAGNNNNNCTC	4 cut(s)	399, 431, 1853, 1885
BpmI	CTGGAG	1 cut(s)	74
Bpu14I	TTCGAA	2 cut(s)	1917, 2425
BpuEI	CTTGAG	1 cut(s)	118
BpuMI	CCSGG	5 cut(s)	552, 771, 1128, 1129, 1275
BsaBI	GATNNNNATC	2 cut(s)	1317, 1802
BsaHI	GRCGYC	1 cut(s)	1839
BsaJI	CCNNGG	6 cut(s)	273, 550, 1126, 1127, 1128, 2595
BsaWI	WCCGGW	2 cut(s)	302, 446
BsaXI	ACNNNNNCTCC	2 cut(s)	1978, 2008
Bsc4I	CCNNNNNNNGG	5 cut(s)	323, 1178, 1209, 1352, 1427
Bse118I	RCCGGY	3 cut(s)	302, 446, 2675
Bse1I	ACTGG	7 cut(s)	91, 523, 530, 688, 1191, 1504, 2055
Bse3DI	GCAATG	3 cut(s)	866, 1042, 2483
Bse8I	GATNNNNATC	2 cut(s)	1317, 1802
BseBI	CCWGG	1 cut(s)	500
BseDI	CCNNGG	6 cut(s)	273, 550, 1126, 1127, 1128, 2595
BseGI	GGATG	4 cut(s)	1112, 2565, 2629, 2705
BseJI	GATNNNNATC	2 cut(s)	1317, 1802
BseLI	CCNNNNNNNGG	5 cut(s)	323, 1178, 1209, 1352, 1427
BseMI	GCAATG	3 cut(s)	866, 1042, 2483
BseMII	CTCAG	6 cut(s)	252, 323, 1163, 1365, 2226, 2445
BseNI	ACTGG	7 cut(s)	91, 523, 530, 688, 1191, 1504, 2055
BseRI	GAGGAG	1 cut(s)	2029
BseSI	GKGCMC	3 cut(s)	88, 1663, 1715
BseXI	GCAGC	5 cut(s)	883, 1150, 2332, 2482, 2618
BseYI	CCCAGC	3 cut(s)	125, 129, 162
BsgI	GTGCAG	1 cut(s)	183
Bsh1236I	CGCG	3 cut(s)	251, 1875, 2352
BshFI	GGCC	4 cut(s)	297, 1548, 2157, 2531
BshNI	GGYRCC	2 cut(s)	611, 1633
BshTI	ACCGGT	2 cut(s)	302, 446
BsiHKAI	GWGCWC	6 cut(s)	88, 1499, 1663, 1715, 1816, 2623
BsiHKCI	CYCGRG	2 cut(s)	1048, 1127
BsiSI	CCGG	7 cut(s)	303, 447, 552, 770, 1128, 1275, 2676
BslFI	GGGAC	4 cut(s)	146, 700, 847, 2490
BslI	CCNNNNNNNGG	5 cut(s)	323, 1178, 1209, 1352, 1427
BsmAI	GTCTC	3 cut(s)	1535, 2011, 2200
BsmFI	GGGAC	4 cut(s)	146, 700, 847, 2490
BsnI	GGCC	4 cut(s)	297, 1548, 2157, 2531
BsoBI	CYCGRG	2 cut(s)	1048, 1127
Bsp119I	TTCGAA	2 cut(s)	1917, 2425
Bsp1286I	GDGCHC	7 cut(s)	88, 258, 1499, 1663, 1715, 1816, 2623
BspANI	GGCC	4 cut(s)	297, 1548, 2157, 2531
BspCNI	CTCAG	6 cut(s)	253, 324, 1162, 1366, 2227, 2444
BspFNI	CGCG	3 cut(s)	251, 1875, 2352
BspLI	GGNNCC	6 cut(s)	613, 1025, 1438, 1635, 2232, 2680
BspMAI	CTGCAG	3 cut(s)	960, 2500, 2550
BspMI	ACCTGC	1 cut(s)	466
BspOI	GCTAGC	1 cut(s)	2471
BspQI	GCTCTTC	2 cut(s)	2261, 2379
BspT104I	TTCGAA	2 cut(s)	1917, 2425
BspT107I	GGYRCC	2 cut(s)	611, 1633
BspTI	CTTAAG	1 cut(s)	1703
BsrBI	CCGCTC	1 cut(s)	540
BsrDI	GCAATG	3 cut(s)	866, 1042, 2483
BsrFI	RCCGGY	3 cut(s)	302, 446, 2675
BsrI	ACTGG	7 cut(s)	91, 523, 530, 688, 1191, 1504, 2055
BssAI	RCCGGY	3 cut(s)	302, 446, 2675
BssECI	CCNNGG	6 cut(s)	273, 550, 1126, 1127, 1128, 2595
BssNAI	GTATAC	2 cut(s)	455, 2278
BssNI	GRCGYC	1 cut(s)	1839
BssSI	CACGAG	1 cut(s)	1550
BssT1I	CCWWGG	1 cut(s)	273
Bst1107I	GTATAC	2 cut(s)	455, 2278
Bst2BI	CACGAG	1 cut(s)	1550
Bst2UI	CCWGG	1 cut(s)	500
Bst4CI	ACNGT	9 cut(s)	185, 567, 838, 864, 1061, 1089, 1668, 1733, 2396
Bst6I	CTCTTC	4 cut(s)	422, 2006, 2261, 2379
BstACI	GRCGYC	1 cut(s)	1839
BstAFI	CTTAAG	1 cut(s)	1703
BstAPI	GCANNNNNTGC	1 cut(s)	177
BstBI	TTCGAA	2 cut(s)	1917, 2425
BstC8I	GCNNGC	3 cut(s)	1778, 2469, 2648
BstDEI	CTNAG	9 cut(s)	261, 332, 556, 737, 1149, 1374, 1967, 2235, 2431
BstDSI	CCRYGG	1 cut(s)	2595
BstENI	CCTNNNNNAGG	1 cut(s)	1176
BstF5I	GGATG	4 cut(s)	1112, 2565, 2629, 2705
BstFNI	CGCG	3 cut(s)	251, 1875, 2352
BstH2I	RGCGCY	2 cut(s)	163, 1202
BstHHI	GCGC	3 cut(s)	162, 1201, 2705
BstMAI	GTCTC	3 cut(s)	1535, 2011, 2200
BstNI	CCWGG	1 cut(s)	500
BstNSI	RCATGY	5 cut(s)	1125, 1334, 1420, 1780, 2195
BstPAI	GACNNNNGTC	1 cut(s)	2204
BstSCI	CCNGG	6 cut(s)	498, 550, 769, 1126, 1127, 1273
BstSFI	CTRYAG	4 cut(s)	956, 1350, 2496, 2546
BstSLI	GKGCMC	3 cut(s)	88, 1663, 1715
BstUI	CGCG	3 cut(s)	251, 1875, 2352
BstV1I	GCAGC	5 cut(s)	883, 1150, 2332, 2482, 2618
BstX2I	RGATCY	6 cut(s)	269, 277, 328, 1745, 1988, 2230
BstXI	CCANNNNNNTGG	1 cut(s)	129
BstYI	RGATCY	6 cut(s)	269, 277, 328, 1745, 1988, 2230
BstZ17I	GTATAC	2 cut(s)	455, 2278
BsuRI	GGCC	4 cut(s)	297, 1548, 2157, 2531
BtgI	CCRYGG	1 cut(s)	2595
BtgZI	GCGATG	3 cut(s)	232, 777, 2286
BtsCI	GGATG	4 cut(s)	1112, 2565, 2629, 2705
BtsI	GCAGTG	1 cut(s)	200
BtsIMutI	CAGTG	7 cut(s)	200, 379, 647, 681, 1184, 1287, 1497
BveI	ACCTGC	1 cut(s)	466
Cac8I	GCNNGC	3 cut(s)	1778, 2469, 2648
CaiI	CAGNNNCTG	1 cut(s)	1936
CfoI	GCGC	3 cut(s)	162, 1201, 2705
Cfr10I	RCCGGY	3 cut(s)	302, 446, 2675
Cfr13I	GGNCC	5 cut(s)	496, 521, 1436, 1546, 2529
Cfr9I	CCCGGG	1 cut(s)	1127
CseI	GACGC	4 cut(s)	952, 1828, 2470, 2598
Csp6I	GTAC	5 cut(s)	612, 997, 1057, 1328, 2357
CspAI	ACCGGT	2 cut(s)	302, 446
CspCI	CAANNNNNGTGG	2 cut(s)	2584, 2619
CviQI	GTAC	5 cut(s)	612, 997, 1057, 1328, 2357
DdeI	CTNAG	9 cut(s)	261, 332, 556, 737, 1149, 1374, 1967, 2235, 2431
Eam1104I	CTCTTC	4 cut(s)	422, 2006, 2261, 2379
EarI	CTCTTC	4 cut(s)	422, 2006, 2261, 2379
Ecl136II	GAGCTC	1 cut(s)	1814
Eco130I	CCWWGG	1 cut(s)	273
Eco147I	AGGCCT	1 cut(s)	297
Eco24I	GRGCYC	2 cut(s)	258, 1816
Eco32I	GATATC	3 cut(s)	54, 1169, 1518
Eco47I	GGWCC	3 cut(s)	496, 521, 1436
Eco47III	AGCGCT	1 cut(s)	161
Eco53kI	GAGCTC	1 cut(s)	1814
Eco57I	CTGAAG	1 cut(s)	355
Eco88I	CYCGRG	2 cut(s)	1048, 1127
EcoICRI	GAGCTC	1 cut(s)	1814
EcoNI	CCTNNNNNAGG	1 cut(s)	1176
EcoRI	GAATTC	2 cut(s)	42, 2660
EcoRII	CCWGG	1 cut(s)	498
EcoRV	GATATC	3 cut(s)	54, 1169, 1518
EcoT14I	CCWWGG	1 cut(s)	273
EcoT38I	GRGCYC	2 cut(s)	258, 1816
ErhI	CCWWGG	1 cut(s)	273
FalI	AAGNNNNNCTT	2 cut(s)	1603, 1635
FaqI	GGGAC	4 cut(s)	146, 700, 847, 2490
FauI	CCCGC	1 cut(s)	244
FblI	GTMKAC	2 cut(s)	454, 2277
FokI	GGATG	4 cut(s)	1099, 2572, 2616, 2712
FriOI	GRGCYC	2 cut(s)	258, 1816
FspBI	CTAG	9 cut(s)	81, 356, 731, 1044, 1098, 1244, 2219, 2316, 2468
GlaI	GCGC	3 cut(s)	161, 1200, 2704
GsaI	CCCAGC	3 cut(s)	129, 133, 166
GsuI	CTGGAG	1 cut(s)	74
HaeII	RGCGCY	2 cut(s)	163, 1202
HaeIII	GGCC	4 cut(s)	297, 1548, 2157, 2531
HapII	CCGG	7 cut(s)	303, 447, 552, 770, 1128, 1275, 2676
HgaI	GACGC	4 cut(s)	952, 1828, 2470, 2598
HhaI	GCGC	3 cut(s)	162, 1201, 2705
Hin1I	GRCGYC	1 cut(s)	1839
Hin6I	GCGC	3 cut(s)	160, 1199, 2703
HinP1I	GCGC	3 cut(s)	160, 1199, 2703
HincII	GTYRAC	1 cut(s)	516
HindII	GTYRAC	1 cut(s)	516
HindIII	AAGCTT	2 cut(s)	1486, 1975
HpaII	CCGG	7 cut(s)	303, 447, 552, 770, 1128, 1275, 2676
HphI	GGTGA	8 cut(s)	205, 292, 436, 790, 932, 1256, 1267, 2377
Hpy99I	CGWCG	2 cut(s)	1844, 2486
HpyAV	CCTTC	7 cut(s)	161, 233, 1172, 1421, 1450, 1756, 2134
HpyCH4III	ACNGT	9 cut(s)	185, 567, 838, 864, 1061, 1089, 1668, 1733, 2396
HpyCH4IV	ACGT	4 cut(s)	117, 222, 1588, 2640
HpyF3I	CTNAG	9 cut(s)	261, 332, 556, 737, 1149, 1374, 1967, 2235, 2431
HpySE526I	ACGT	4 cut(s)	117, 222, 1588, 2640
Hsp92I	GRCGYC	1 cut(s)	1839
HspAI	GCGC	3 cut(s)	160, 1199, 2703
KpnI	GGTACC	1 cut(s)	615
LguI	GCTCTTC	2 cut(s)	2261, 2379
LmnI	GCTCC	8 cut(s)	111, 157, 641, 772, 1676, 2464, 2626, 2684
Lsp1109I	GCAGC	5 cut(s)	883, 1150, 2332, 2482, 2618
LweI	GCATC	6 cut(s)	67, 180, 1923, 2154, 2591, 2690
MaeI	CTAG	9 cut(s)	81, 356, 731, 1044, 1098, 1244, 2219, 2316, 2468
MaeII	ACGT	4 cut(s)	117, 222, 1588, 2640
MaeIII	GTNAC	4 cut(s)	193, 740, 1225, 1284
MbiI	CCGCTC	1 cut(s)	540
MfeI	CAATTG	3 cut(s)	2005, 2255, 2325
MflI	RGATCY	6 cut(s)	269, 277, 328, 1745, 1988, 2230
MhlI	GDGCHC	7 cut(s)	88, 258, 1499, 1663, 1715, 1816, 2623
MlyI	GAGTC	6 cut(s)	712, 1060, 2013, 2194, 2242, 2298
MmeI	TCCRAC	1 cut(s)	504
MroXI	GAANNNNTTC	2 cut(s)	440, 2541
MslI	CAYNNNNRTG	3 cut(s)	72, 1887, 2094
MspA1I	CMGCKG	4 cut(s)	129, 2181, 2495, 2694
MspCI	CTTAAG	1 cut(s)	1703
MspI	CCGG	7 cut(s)	303, 447, 552, 770, 1128, 1275, 2676
MspR9I	CCNGG	6 cut(s)	500, 552, 771, 1128, 1129, 1275
MunI	CAATTG	3 cut(s)	2005, 2255, 2325
MvaI	CCWGG	1 cut(s)	500
MvnI	CGCG	3 cut(s)	251, 1875, 2352
NciI	CCSGG	5 cut(s)	552, 771, 1128, 1129, 1275
NheI	GCTAGC	1 cut(s)	2467
NlaIV	GGNNCC	6 cut(s)	613, 1025, 1438, 1635, 2232, 2680
NmeAIII	GCCGAG	2 cut(s)	1795, 2018
NmuCI	GTSAC	3 cut(s)	193, 1225, 1284
NspI	RCATGY	5 cut(s)	1125, 1334, 1420, 1780, 2195
NspV	TTCGAA	2 cut(s)	1917, 2425
PaeI	GCATGC	1 cut(s)	1780
PaeR7I	CTCGAG	1 cut(s)	1048
PceI	AGGCCT	1 cut(s)	297
PciI	ACATGT	2 cut(s)	1330, 2191
PciSI	GCTCTTC	2 cut(s)	2261, 2379
PcsI	WCGNNNNNNNCGW	2 cut(s)	755, 2422
PdmI	GAANNNNTTC	2 cut(s)	440, 2541
PfeI	GAWTC	5 cut(s)	678, 970, 1040, 1908, 2489
PinAI	ACCGGT	2 cut(s)	302, 446
PleI	GAGTC	6 cut(s)	712, 1059, 2013, 2194, 2242, 2297
PpsI	GAGTC	6 cut(s)	712, 1059, 2013, 2194, 2242, 2297
PscI	ACATGT	2 cut(s)	1330, 2191
PshAI	GACNNNNGTC	1 cut(s)	2204
PshBI	ATTAAT	1 cut(s)	1010
PsiI	TTATAA	1 cut(s)	993
Psp124BI	GAGCTC	1 cut(s)	1816
Psp1406I	AACGTT	2 cut(s)	117, 2640
Psp6I	CCWGG	1 cut(s)	498
PspFI	CCCAGC	3 cut(s)	125, 129, 162
PspGI	CCWGG	1 cut(s)	498
PspN4I	GGNNCC	6 cut(s)	613, 1025, 1438, 1635, 2232, 2680
PspPI	GGNCC	5 cut(s)	496, 521, 1436, 1546, 2529
PspXI	VCTCGAGB	1 cut(s)	1048
PstI	CTGCAG	3 cut(s)	960, 2500, 2550
PstNI	CAGNNNCTG	1 cut(s)	1936
PsuI	RGATCY	6 cut(s)	269, 277, 328, 1745, 1988, 2230
PvuII	CAGCTG	2 cut(s)	129, 2495
RsaI	GTAC	5 cut(s)	613, 998, 1058, 1329, 2358
RsaNI	GTAC	5 cut(s)	612, 997, 1057, 1328, 2357
RseI	CAYNNNNRTG	3 cut(s)	72, 1887, 2094
SacI	GAGCTC	1 cut(s)	1816
SapI	GCTCTTC	2 cut(s)	2261, 2379
Sau96I	GGNCC	5 cut(s)	496, 521, 1436, 1546, 2529
SchI	GAGTC	6 cut(s)	712, 1060, 2013, 2194, 2242, 2298
ScrFI	CCNGG	6 cut(s)	500, 552, 771, 1128, 1129, 1275
SduI	GDGCHC	7 cut(s)	88, 258, 1499, 1663, 1715, 1816, 2623
SfaNI	GCATC	6 cut(s)	67, 180, 1923, 2154, 2591, 2690
SfcI	CTRYAG	4 cut(s)	956, 1350, 2496, 2546
Sfr274I	CTCGAG	1 cut(s)	1048
SfuI	TTCGAA	2 cut(s)	1917, 2425
SinI	GGWCC	3 cut(s)	496, 521, 1436
SlaI	CTCGAG	1 cut(s)	1048
SmaI	CCCGGG	1 cut(s)	1129
SmiMI	CAYNNNNRTG	3 cut(s)	72, 1887, 2094
SmlI	CTYRAG	3 cut(s)	97, 1048, 1703
SmoI	CTYRAG	3 cut(s)	97, 1048, 1703
SphI	GCATGC	1 cut(s)	1780
SseBI	AGGCCT	1 cut(s)	297
SspI	AATATT	2 cut(s)	705, 2113
SspMI	CTAG	9 cut(s)	81, 356, 731, 1044, 1098, 1244, 2219, 2316, 2468
SstI	GAGCTC	1 cut(s)	1816
StuI	AGGCCT	1 cut(s)	297
StyD4I	CCNGG	6 cut(s)	498, 550, 769, 1126, 1127, 1273
StyI	CCWWGG	1 cut(s)	273
TaaI	ACNGT	9 cut(s)	185, 567, 838, 864, 1061, 1089, 1668, 1733, 2396
TaiI	ACGT	4 cut(s)	120, 225, 1591, 2643
TaqII	GACCGA	1 cut(s)	2276
TatI	WGTACW	2 cut(s)	996, 1327
TauI	GCSGC	5 cut(s)	7, 369, 543, 1423, 1755
TfiI	GAWTC	5 cut(s)	678, 970, 1040, 1908, 2489
TscAI	CASTG	7 cut(s)	207, 379, 654, 688, 1191, 1287, 1504
TseFI	GTSAC	3 cut(s)	193, 1225, 1284
TseI	GCWGC	7 cut(s)	7, 10, 871, 1138, 2320, 2495, 2606
Tsp45I	GTSAC	3 cut(s)	193, 1225, 1284
TspGWI	ACGGA	5 cut(s)	700, 747, 1044, 1118, 2612
TspMI	CCCGGG	1 cut(s)	1127
TspRI	CASTG	7 cut(s)	207, 379, 654, 688, 1191, 1287, 1504
Vha464I	CTTAAG	1 cut(s)	1703
VneI	GTGCAC	3 cut(s)	84, 1659, 1711
VpaK11BI	GGWCC	3 cut(s)	496, 521, 1436
VspI	ATTAAT	1 cut(s)	1010
XagI	CCTNNNNNAGG	1 cut(s)	1176
XapI	RAATTY	3 cut(s)	42, 1563, 2660
XbaI	TCTAGA	1 cut(s)	730
XceI	RCATGY	5 cut(s)	1125, 1334, 1420, 1780, 2195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2030
XhoI	CTCGAG	1 cut(s)	1048
XmaI	CCCGGG	1 cut(s)	1127
XmiI	GTMKAC	2 cut(s)	454, 2277
XmnI	GAANNNNTTC	2 cut(s)	440, 2541
XspI	CTAG	9 cut(s)	81, 356, 731, 1044, 1098, 1244, 2219, 2316, 2468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.