Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003096

Physical Location & Seq

Genomic Coordinates Reverse (-)

27404103 .. 27407919

Gene Structure 3817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1183000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGATGTTTTTCTTTTCCCAAGTTTATAAAACTTTGGTTTTTCTCAGGGCTTTGAATACGAATAACTTCACCGGTAGTATACCTGCTTCTTTGGGTAATCTCTCCAACCTCTATTGGCTGGACCTGGCAGACAATCAGTTGACTGGACCTCTCCCAGTTTCGAGCGGCACTACCCCCGGCTTAGACCAACTGTTTAACGCTAAACATTTCCATTTGAACCAGAACCAGCTTTCAGGTACCATTCCACCCAGACTTTTCAGCTCCAAGAAGACACTGATCCATGTATTGTTTGATGGAAATGGATTCACTGGGACTATTCCATCAACAATATTTTCCGTTCAGACTCTCGACATTCTGCGGCTTGATAGGAATGCTCTGACGGGACCAGTCTCCTCAAATATCTCCAACCTAAAAAATCTCAGTAAATTAGACCTTAGTTACAACTCGTTTGATCCGTCAGAAGCTCCGGGTTGGTTCTCAACCTTGCCATCGCTCACCACTATCGTTTTAGAAGATGTAGCTCTTCAAGGGACTGTGCCAGAGAAGATGTTCAGCATACCGTCATTGCAGCAAGTGAAAATGAAAAACAATGCGTTTAACGAGACATTTAACATGGGTGATACAATCAGTCCGCAACTGAGGCTTGTTGATCTGCAGAACAATAAGATTCAGAAAATAACACCGGGTTATGAGTACAAACATACATTAATACTTGTTGGGAACCCATTCTGCAATGAATCTAGCTCGAGTCAGTACTGTCAGCCTCTACAATCAAATACAAAAACGTATGCTACTAGAACGGATTGTCCATCCATTACATGCCCCGGGGGTCAGCAGCCAAGCCCTCAGAGTTGTCAATGTGGATATCCTTTTGAAGGAACACTGGATTTCCGAGCGCCCACTTTCAGGGATTTGACAAATGGGACTCTGTTTCAATCCCTAGAACAATGGGTGAAACTTCACCTCACTCCCGGTTCAGTGTCACTTGATAACCCTTTATTCGACAACAATGACCATCTTAAAGTACATGTGGCTCTCTTTCCACCCACTGGAACATATTTTAATAGGTCTGAGATTAGCAGGATTGGTTTTGTTATGAGCAGTCAGCCTAACATGCCGCCAGATGGGTTTGGACCCTTCGTTTTTATTCCAGCTCAATATAATTTCCCAGTTAGTTGTCTCGTTCTTGTTGTGGGCCTCGTGGTAGTAGGAATTTACGCCATTAGGCAAAGGAAACGTGCGGAAAGAGCCATTGGTTTAAGCAGACCTTTCGCTTCTTGGGTGCCAAGCGGAAATGATAGTGGCGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCGTATGATGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAGTAATGAGATAGGATCTGGCGGCTACGGGAAGGTTTACAGGGGCATGCTTTCTGATGGACAAGTGATAGCAATCAAAAGAGCTCAGCAAGGATCAATGCAGGGAGGTGTCGAGTTCACGACTGAAATTGAGTTGCTCTCTCGCGTTCATCACAAAAATGTTGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTATATGCCTAATGGAACACTTAGGGAAAGCTTGTCAGGGAGATCTGGTATTCATCTCGATTGGAAGAGGCGACTCCAAATCACTCTTGGCTCGGCAAGAGGACTGGCTTACCTACATGAGCTTGCAAATCCTCCTATAATTCACAGAGATGTGAAGTCCACCAATATTCTATTAGATGAACATTTAACGGCGAAGGTTGCAGATTTTGGCCTGTCGAAGCTGGTCAATGACAGTGGAAAAGGACATGTATCGACTCAGGTCAAAGGAACAATGGGCTATCTGGATCCTGAGTACTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCAGCCAATCGAGAAGGGAAAATACATTGTCCGCGAGGTACAGTTGGTGATGGACAAGAATGACGAAGAGCATTACGGTTTGAGGGAGTTAATAGATCGAAACATTCGAAACTCAGGAACTCTTATTGGGTTTGGGAGGTTCTTGGAGCTAGCCATGCAATGCGTCGAAGAATCAGCTGCAGATCGTCCCACGATGAGTGAAGTTGTGAAGGCTATCGAAACCATTCTGCAGAACGGTGGGATGAACACAAACTCAACATCAGCATCTTCATCAGCCACAGAGTTTGCAGCATCAAAAGGTGCTCCGAAACATCCCTACAACGATGGCTTGCCGAAAAAGGAATTCAATGACAGCACCGGCTCCTTTGATTACAGCGGTGGATACGCTGTTTCAGCGAAAATCAAACCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 Pkinase_Tyr

Protein Analysis

794

Amino Acids

87.52

Weight (kDa)

7.24

Isoelectric Point (pI)

42.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	459 - 726	6.8e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	462 - 727	9e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003096)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79620 AT1G79620 AT1G79620 AT1G79620 AT1G79620
fragaria_vesca	FvH4_6g09020 FvH4_6g09020
malus_domestica	MD04G1181800.v1.1 MD04G1182200.v1.1 MD04G1183000.v1.1 MD04G1183100.v1.1 MD12G1196600.v1.1
prunus_persica	Prupe.6G301100_v2.0.a1 Prupe.6G301100_v2.0.a1 Prupe.6G301200_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301500_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1 Prupe.6G301600_v2.0.a1
pyrus_communis	pycom04g16070 pycom04g16150 pycom04g16170 pycom12g18280
rosa_chinensis	RchiOBHm_Chr3g0459271
rosa_laevigata	RLG00000025098
rosa_multiflora	Rmu_sc0006220.1_g000001 Rmu_sc0010702.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420350
rosa_rugosa	Rorug03G0034900 Rorug03G0035000
rosa_samantha	Rh3AG092700 Rh3BG095800 Rh3CG096800 Rh3DG096400
rosa_wichuraiana	Rw3G007970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	27
Acc36I	ACCTGC	1 cut(s)	91
Acc65I	GGTACC	1 cut(s)	236
AccB1I	GGYRCC	2 cut(s)	236, 1282
AccB7I	CCANNNNNTGG	1 cut(s)	1049
AccBSI	CCGCTC	1 cut(s)	165
AccI	GTMKAC	2 cut(s)	79, 1926
AccII	CGCG	2 cut(s)	1524, 2001
AclWI	GGATC	6 cut(s)	271, 446, 1402, 1480, 1874, 1887
AcsI	RAATTY	2 cut(s)	1212, 2309
AfaI	GTAC	6 cut(s)	238, 695, 755, 1026, 1889, 2007
AfiI	CCNNNNNNNGG	4 cut(s)	875, 906, 1049, 1124
AflII	CTTAAG	1 cut(s)	1352
AflIII	ACRYGT	2 cut(s)	1027, 1840
AgeI	ACCGGT	1 cut(s)	71
AgsI	TTSAA	7 cut(s)	55, 217, 527, 875, 935, 1952, 2314
AjnI	CCWGG	1 cut(s)	123
AjuI	GAANNNNNNNTTGG	2 cut(s)	1236, 1268
AloI	GAACNNNNNNTCC	2 cut(s)	790, 822
Alw21I	GWGCWC	4 cut(s)	1312, 1364, 1465, 2272
Alw26I	GTCTC	3 cut(s)	394, 596, 1184
Alw44I	GTGCAC	2 cut(s)	1308, 1360
AlwI	GGATC	6 cut(s)	271, 446, 1402, 1480, 1874, 1887
AlwNI	CAGNNNCTG	1 cut(s)	1585
Ama87I	CYCGRG	2 cut(s)	745, 824
AoxI	GGCC	2 cut(s)	1195, 1804
ApaLI	GTGCAC	2 cut(s)	1308, 1360
ApeKI	GCWGC	5 cut(s)	568, 835, 1969, 2144, 2255
ApoI	RAATTY	2 cut(s)	1212, 2309
AseI	ATTAAT	1 cut(s)	707
AsiGI	ACCGGT	1 cut(s)	71
Asp700I	GAANNNNTTC	4 cut(s)	65, 548, 725, 2190
Asp718I	GGTACC	1 cut(s)	236
AspLEI	GCGC	1 cut(s)	898
AspS9I	GGNCC	5 cut(s)	121, 146, 383, 1133, 1195
AsuC2I	CCSGG	6 cut(s)	177, 468, 684, 825, 826, 972
AsuHPI	GGTGA	6 cut(s)	61, 487, 629, 953, 964, 2026
AsuII	TTCGAA	2 cut(s)	1566, 2074
AsuNHI	GCTAGC	1 cut(s)	2116
AvaI	CYCGRG	2 cut(s)	745, 824
AvaII	GGWCC	4 cut(s)	121, 146, 383, 1133
BaeGI	GKGCMC	2 cut(s)	1312, 1364
BamHI	GGATCC	1 cut(s)	1879
BanI	GGYRCC	2 cut(s)	236, 1282
BanII	GRGCYC	1 cut(s)	1465
BarI	GAAGNNNNNNTAC	4 cut(s)	70, 102, 1973, 2005
BauI	CACGAG	1 cut(s)	1199
BbsI	GAAGAC	1 cut(s)	275
Bbv12I	GWGCWC	4 cut(s)	1312, 1364, 1465, 2272
BbvI	GCAGC	5 cut(s)	580, 847, 1981, 2131, 2267
BceAI	ACGGC	1 cut(s)	1800
BcgI	CGANNNNNNTGC	2 cut(s)	1252, 1286
BciT130I	CCWGG	1 cut(s)	125
BciVI	GTATCC	1 cut(s)	2342
BcnI	CCSGG	6 cut(s)	177, 468, 684, 825, 826, 972
BcoDI	GTCTC	3 cut(s)	394, 596, 1184
BfaI	CTAG	5 cut(s)	741, 795, 941, 1965, 2117
BfmI	CTRYAG	3 cut(s)	653, 2145, 2195
BfoI	RGCGCY	1 cut(s)	899
BfrI	CTTAAG	1 cut(s)	1352
BfuAI	ACCTGC	1 cut(s)	91
BfuI	GTATCC	1 cut(s)	2342
BglII	AGATCT	1 cut(s)	1637
BisI	GCNGC	9 cut(s)	166, 359, 569, 836, 1118, 1402, 1970, 2145, 2256
BlpI	GCTNAGC	1 cut(s)	1464
BlsI	GCNGC	9 cut(s)	167, 360, 570, 837, 1119, 1403, 1971, 2146, 2257
BmcAI	AGTACT	2 cut(s)	755, 1889
Bme1390I	CCNGG	7 cut(s)	125, 177, 468, 684, 825, 826, 972
Bme18I	GGWCC	4 cut(s)	121, 146, 383, 1133
BmeT110I	CYCGRG	2 cut(s)	745, 824
BmgT120I	GGNCC	5 cut(s)	121, 146, 383, 1133, 1195
BmiI	GGNNCC	7 cut(s)	238, 384, 722, 1135, 1284, 1881, 2329
BmrFI	CCNGG	7 cut(s)	125, 177, 468, 684, 825, 826, 972
BmrI	ACTGGG	3 cut(s)	149, 318, 1163
BmsI	GCATC	3 cut(s)	1572, 2240, 2267
BmtI	GCTAGC	1 cut(s)	2120
BmuI	ACTGGG	3 cut(s)	149, 318, 1163
BoxI	GACNNNNGTC	1 cut(s)	1853
BpiI	GAAGAC	1 cut(s)	275
BplI	GAGNNNNNCTC	2 cut(s)	1502, 1534
Bpu1102I	GCTNAGC	1 cut(s)	1464
Bpu14I	TTCGAA	2 cut(s)	1566, 2074
BpuMI	CCSGG	6 cut(s)	177, 468, 684, 825, 826, 972
BsaBI	GATNNNNATC	1 cut(s)	1451
BsaJI	CCNNGG	4 cut(s)	175, 823, 824, 825
BsaWI	WCCGGW	1 cut(s)	71
BsaXI	ACNNNNNCTCC	2 cut(s)	1627, 1657
Bsc4I	CCNNNNNNNGG	4 cut(s)	875, 906, 1049, 1124
Bse118I	RCCGGY	2 cut(s)	71, 2324
Bse1I	ACTGG	8 cut(s)	148, 155, 313, 386, 888, 1054, 1169, 1704
Bse3DI	GCAATG	3 cut(s)	563, 739, 2132
Bse8I	GATNNNNATC	1 cut(s)	1451
BseBI	CCWGG	1 cut(s)	125
BseDI	CCNNGG	4 cut(s)	175, 823, 824, 825
BseGI	GGATG	3 cut(s)	809, 2214, 2278
BseJI	GATNNNNATC	1 cut(s)	1451
BseLI	CCNNNNNNNGG	4 cut(s)	875, 906, 1049, 1124
BseMI	GCAATG	3 cut(s)	563, 739, 2132
BseMII	CTCAG	9 cut(s)	58, 433, 629, 860, 1062, 1478, 1865, 1875, 2094
BseNI	ACTGG	8 cut(s)	148, 155, 313, 386, 888, 1054, 1169, 1704
BseRI	GAGGAG	1 cut(s)	382
BseSI	GKGCMC	2 cut(s)	1312, 1364
BseXI	GCAGC	5 cut(s)	580, 847, 1981, 2131, 2267
Bsh1236I	CGCG	2 cut(s)	1524, 2001
BshFI	GGCC	2 cut(s)	1197, 1806
BshNI	GGYRCC	2 cut(s)	236, 1282
BshTI	ACCGGT	1 cut(s)	71
BsiHKAI	GWGCWC	4 cut(s)	1312, 1364, 1465, 2272
BsiHKCI	CYCGRG	2 cut(s)	745, 824
BsiSI	CCGG	7 cut(s)	72, 177, 467, 683, 825, 972, 2325
BslFI	GGGAC	5 cut(s)	325, 396, 544, 937, 2139
BslI	CCNNNNNNNGG	4 cut(s)	875, 906, 1049, 1124
BsmAI	GTCTC	3 cut(s)	394, 596, 1184
BsmFI	GGGAC	5 cut(s)	325, 396, 544, 937, 2139
BsmI	GAATGC	1 cut(s)	376
BsnI	GGCC	2 cut(s)	1197, 1806
BsoBI	CYCGRG	2 cut(s)	745, 824
Bsp119I	TTCGAA	2 cut(s)	1566, 2074
Bsp1286I	GDGCHC	4 cut(s)	1312, 1364, 1465, 2272
Bsp143I	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
Bsp1720I	GCTNAGC	1 cut(s)	1464
BspANI	GGCC	2 cut(s)	1197, 1806
BspCNI	CTCAG	9 cut(s)	57, 432, 630, 859, 1063, 1477, 1864, 1876, 2093
BspFNI	CGCG	2 cut(s)	1524, 2001
BspLI	GGNNCC	7 cut(s)	238, 384, 722, 1135, 1284, 1881, 2329
BspMAI	CTGCAG	3 cut(s)	657, 2149, 2199
BspMI	ACCTGC	1 cut(s)	91
BspOI	GCTAGC	1 cut(s)	2120
BspPI	GGATC	6 cut(s)	271, 446, 1402, 1480, 1874, 1887
BspQI	GCTCTTC	3 cut(s)	528, 1910, 2028
BspT104I	TTCGAA	2 cut(s)	1566, 2074
BspT107I	GGYRCC	2 cut(s)	236, 1282
BspTI	CTTAAG	1 cut(s)	1352
BsrBI	CCGCTC	1 cut(s)	165
BsrDI	GCAATG	3 cut(s)	563, 739, 2132
BsrFI	RCCGGY	2 cut(s)	71, 2324
BsrI	ACTGG	8 cut(s)	148, 155, 313, 386, 888, 1054, 1169, 1704
BssAI	RCCGGY	2 cut(s)	71, 2324
BssECI	CCNNGG	4 cut(s)	175, 823, 824, 825
BssMI	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
BssNAI	GTATAC	2 cut(s)	80, 1927
BssSI	CACGAG	1 cut(s)	1199
Bst1107I	GTATAC	2 cut(s)	80, 1927
Bst2BI	CACGAG	1 cut(s)	1199
Bst2UI	CCWGG	1 cut(s)	125
Bst6I	CTCTTC	4 cut(s)	528, 1655, 1910, 2028
BstAFI	CTTAAG	1 cut(s)	1352
BstBI	TTCGAA	2 cut(s)	1566, 2074
BstC8I	GCNNGC	4 cut(s)	1427, 1719, 2118, 2297
BstENI	CCTNNNNNAGG	1 cut(s)	873
BstF5I	GGATG	3 cut(s)	809, 2214, 2278
BstFNI	CGCG	2 cut(s)	1524, 2001
BstH2I	RGCGCY	1 cut(s)	899
BstHHI	GCGC	1 cut(s)	898
BstKTI	GATC	9 cut(s)	279, 454, 652, 1397, 1475, 1640, 1882, 2065, 2152
BstMAI	GTCTC	3 cut(s)	394, 596, 1184
BstMBI	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
BstMWI	GCNNNNNNNGC	6 cut(s)	640, 1114, 1247, 1793, 2122, 2360
BstNI	CCWGG	1 cut(s)	125
BstNSI	RCATGY	5 cut(s)	822, 1031, 1117, 1429, 1844
BstPAI	GACNNNNGTC	1 cut(s)	1853
BstSCI	CCNGG	7 cut(s)	123, 175, 466, 682, 823, 824, 970
BstSFI	CTRYAG	3 cut(s)	653, 2145, 2195
BstSLI	GKGCMC	2 cut(s)	1312, 1364
BstUI	CGCG	2 cut(s)	1524, 2001
BstV1I	GCAGC	5 cut(s)	580, 847, 1981, 2131, 2267
BstV2I	GAAGAC	1 cut(s)	275
BstX2I	RGATCY	3 cut(s)	1394, 1637, 1879
BstYI	RGATCY	3 cut(s)	1394, 1637, 1879
BstZ17I	GTATAC	2 cut(s)	80, 1927
BsuI	GTATCC	1 cut(s)	2342
BsuRI	GGCC	2 cut(s)	1197, 1806
BtgZI	GCGATG	2 cut(s)	474, 1935
BtsCI	GGATG	3 cut(s)	809, 2214, 2278
BtsIMutI	CAGTG	6 cut(s)	272, 306, 881, 984, 1047, 1834
BveI	ACCTGC	1 cut(s)	91
Cac8I	GCNNGC	4 cut(s)	1427, 1719, 2118, 2297
CaiI	CAGNNNCTG	1 cut(s)	1585
CfoI	GCGC	1 cut(s)	898
Cfr10I	RCCGGY	2 cut(s)	71, 2324
Cfr13I	GGNCC	5 cut(s)	121, 146, 383, 1133, 1195
Cfr9I	CCCGGG	1 cut(s)	824
CseI	GACGC	1 cut(s)	2119
Csp6I	GTAC	6 cut(s)	237, 694, 754, 1025, 1888, 2006
CspAI	ACCGGT	1 cut(s)	71
CspCI	CAANNNNNGTGG	4 cut(s)	1810, 1845, 2233, 2268
CviQI	GTAC	6 cut(s)	237, 694, 754, 1025, 1888, 2006
DpnI	GATC	9 cut(s)	278, 453, 651, 1396, 1474, 1639, 1881, 2064, 2151
DpnII	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
Eam1104I	CTCTTC	4 cut(s)	528, 1655, 1910, 2028
EarI	CTCTTC	4 cut(s)	528, 1655, 1910, 2028
Ecl136II	GAGCTC	1 cut(s)	1463
Eco24I	GRGCYC	1 cut(s)	1465
Eco32I	GATATC	1 cut(s)	866
Eco47I	GGWCC	4 cut(s)	121, 146, 383, 1133
Eco53kI	GAGCTC	1 cut(s)	1463
Eco88I	CYCGRG	2 cut(s)	745, 824
EcoICRI	GAGCTC	1 cut(s)	1463
EcoNI	CCTNNNNNAGG	1 cut(s)	873
EcoRI	GAATTC	1 cut(s)	2309
EcoRII	CCWGG	1 cut(s)	123
EcoRV	GATATC	1 cut(s)	866
EcoT38I	GRGCYC	1 cut(s)	1465
FalI	AAGNNNNNCTT	2 cut(s)	1252, 1284
FaqI	GGGAC	5 cut(s)	325, 396, 544, 937, 2139
FblI	GTMKAC	2 cut(s)	79, 1926
Fnu4HI	GCNGC	9 cut(s)	166, 359, 569, 836, 1118, 1402, 1970, 2145, 2256
FokI	GGATG	3 cut(s)	796, 2221, 2265
FriOI	GRGCYC	1 cut(s)	1465
Fsp4HI	GCNGC	9 cut(s)	166, 359, 569, 836, 1118, 1402, 1970, 2145, 2256
FspBI	CTAG	5 cut(s)	741, 795, 941, 1965, 2117
GlaI	GCGC	1 cut(s)	897
GluI	GCNGC	9 cut(s)	166, 359, 569, 836, 1118, 1402, 1970, 2145, 2256
HaeII	RGCGCY	1 cut(s)	899
HaeIII	GGCC	2 cut(s)	1197, 1806
HapII	CCGG	7 cut(s)	72, 177, 467, 683, 825, 972, 2325
HgaI	GACGC	1 cut(s)	2119
HhaI	GCGC	1 cut(s)	898
Hin6I	GCGC	1 cut(s)	896
HinP1I	GCGC	1 cut(s)	896
HincII	GTYRAC	1 cut(s)	141
HindII	GTYRAC	1 cut(s)	141
HindIII	AAGCTT	1 cut(s)	1624
HpaII	CCGG	7 cut(s)	72, 177, 467, 683, 825, 972, 2325
HphI	GGTGA	6 cut(s)	61, 487, 629, 953, 964, 2026
Hpy166II	GTNNAC	9 cut(s)	80, 141, 1310, 1362, 1417, 1497, 1591, 1755, 1927
Hpy188III	TCNNGA	7 cut(s)	347, 1498, 1652, 1877, 1883, 1978, 2082
Hpy8I	GTNNAC	9 cut(s)	80, 141, 1310, 1362, 1417, 1497, 1591, 1755, 1927
Hpy99I	CGWCG	1 cut(s)	2135
HpyAV	CCTTC	6 cut(s)	869, 1147, 1405, 1783, 1975, 2170
HpyCH4IV	ACGT	2 cut(s)	785, 1237
HpyF10VI	GCNNNNNNNGC	6 cut(s)	640, 1114, 1247, 1793, 2122, 2360
HpySE526I	ACGT	2 cut(s)	785, 1237
HspAI	GCGC	1 cut(s)	896
KpnI	GGTACC	1 cut(s)	240
Kzo9I	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
LguI	GCTCTTC	3 cut(s)	528, 1910, 2028
LmnI	GCTCC	6 cut(s)	266, 469, 1325, 2113, 2275, 2333
Lsp1109I	GCAGC	5 cut(s)	580, 847, 1981, 2131, 2267
LweI	GCATC	3 cut(s)	1572, 2240, 2267
MaeI	CTAG	5 cut(s)	741, 795, 941, 1965, 2117
MaeII	ACGT	2 cut(s)	785, 1237
MaeIII	GTNAC	2 cut(s)	437, 981
MalI	GATC	9 cut(s)	278, 453, 651, 1396, 1474, 1639, 1881, 2064, 2151
MbiI	CCGCTC	1 cut(s)	165
MboI	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
MboII	GAAGA	9 cut(s)	280, 515, 524, 556, 1672, 1927, 2045, 2147, 2226
MfeI	CAATTG	1 cut(s)	1904
MflI	RGATCY	3 cut(s)	1394, 1637, 1879
MhlI	GDGCHC	4 cut(s)	1312, 1364, 1465, 2272
MluCI	AATT	8 cut(s)	425, 1162, 1212, 1369, 1506, 1734, 1904, 2309
MlyI	GAGTC	7 cut(s)	337, 757, 919, 1662, 1843, 1891, 1947
MmeI	TCCRAC	2 cut(s)	129, 429
MroXI	GAANNNNTTC	4 cut(s)	65, 548, 725, 2190
MslI	CAYNNNNRTG	2 cut(s)	1536, 1743
MspA1I	CMGCKG	2 cut(s)	2144, 2343
MspCI	CTTAAG	1 cut(s)	1352
MspI	CCGG	7 cut(s)	72, 177, 467, 683, 825, 972, 2325
MspR9I	CCNGG	7 cut(s)	125, 177, 468, 684, 825, 826, 972
MunI	CAATTG	1 cut(s)	1904
Mva1269I	GAATGC	1 cut(s)	376
MvaI	CCWGG	1 cut(s)	125
MvnI	CGCG	2 cut(s)	1524, 2001
MwoI	GCNNNNNNNGC	6 cut(s)	640, 1114, 1247, 1793, 2122, 2360
NciI	CCSGG	6 cut(s)	177, 468, 684, 825, 826, 972
NdeII	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
NheI	GCTAGC	1 cut(s)	2116
NlaIV	GGNNCC	7 cut(s)	238, 384, 722, 1135, 1284, 1881, 2329
NmeAIII	GCCGAG	1 cut(s)	1667
NmuCI	GTSAC	1 cut(s)	981
NspI	RCATGY	5 cut(s)	822, 1031, 1117, 1429, 1844
NspV	TTCGAA	2 cut(s)	1566, 2074
PaeI	GCATGC	1 cut(s)	1429
PaeR7I	CTCGAG	1 cut(s)	745
PciI	ACATGT	2 cut(s)	1027, 1840
PciSI	GCTCTTC	3 cut(s)	528, 1910, 2028
PcsI	WCGNNNNNNNCGW	2 cut(s)	452, 2071
PctI	GAATGC	1 cut(s)	376
PdmI	GAANNNNTTC	4 cut(s)	65, 548, 725, 2190
PfeI	GAWTC	5 cut(s)	303, 667, 737, 1557, 2138
PflMI	CCANNNNNTGG	1 cut(s)	1049
PinAI	ACCGGT	1 cut(s)	71
PkrI	GCNGC	9 cut(s)	167, 360, 570, 837, 1119, 1403, 1971, 2146, 2257
PleI	GAGTC	7 cut(s)	337, 756, 919, 1662, 1843, 1891, 1946
PpsI	GAGTC	7 cut(s)	337, 756, 919, 1662, 1843, 1891, 1946
PscI	ACATGT	2 cut(s)	1027, 1840
PshAI	GACNNNNGTC	1 cut(s)	1853
PshBI	ATTAAT	1 cut(s)	707
PsiI	TTATAA	1 cut(s)	27
Psp124BI	GAGCTC	1 cut(s)	1465
Psp6I	CCWGG	1 cut(s)	123
PspGI	CCWGG	1 cut(s)	123
PspN4I	GGNNCC	7 cut(s)	238, 384, 722, 1135, 1284, 1881, 2329
PspPI	GGNCC	5 cut(s)	121, 146, 383, 1133, 1195
PspXI	VCTCGAGB	1 cut(s)	745
PstI	CTGCAG	3 cut(s)	657, 2149, 2199
PstNI	CAGNNNCTG	1 cut(s)	1585
PsuI	RGATCY	3 cut(s)	1394, 1637, 1879
PvuII	CAGCTG	1 cut(s)	2144
RsaI	GTAC	6 cut(s)	238, 695, 755, 1026, 1889, 2007
RsaNI	GTAC	6 cut(s)	237, 694, 754, 1025, 1888, 2006
RseI	CAYNNNNRTG	2 cut(s)	1536, 1743
SacI	GAGCTC	1 cut(s)	1465
SapI	GCTCTTC	3 cut(s)	528, 1910, 2028
SatI	GCNGC	9 cut(s)	166, 359, 569, 836, 1118, 1402, 1970, 2145, 2256
Sau3AI	GATC	9 cut(s)	276, 451, 649, 1394, 1472, 1637, 1879, 2062, 2149
Sau96I	GGNCC	5 cut(s)	121, 146, 383, 1133, 1195
ScaI	AGTACT	2 cut(s)	755, 1889
SchI	GAGTC	7 cut(s)	337, 757, 919, 1662, 1843, 1891, 1947
ScrFI	CCNGG	7 cut(s)	125, 177, 468, 684, 825, 826, 972
SduI	GDGCHC	4 cut(s)	1312, 1364, 1465, 2272
SfaNI	GCATC	3 cut(s)	1572, 2240, 2267
SfcI	CTRYAG	3 cut(s)	653, 2145, 2195
Sfr274I	CTCGAG	1 cut(s)	745
SfuI	TTCGAA	2 cut(s)	1566, 2074
SinI	GGWCC	4 cut(s)	121, 146, 383, 1133
SlaI	CTCGAG	1 cut(s)	745
SmaI	CCCGGG	1 cut(s)	826
SmiMI	CAYNNNNRTG	2 cut(s)	1536, 1743
SmlI	CTYRAG	2 cut(s)	745, 1352
SmoI	CTYRAG	2 cut(s)	745, 1352
SphI	GCATGC	1 cut(s)	1429
Sse9I	AATT	8 cut(s)	425, 1162, 1212, 1369, 1506, 1734, 1904, 2309
SspI	AATATT	2 cut(s)	330, 1762
SspMI	CTAG	5 cut(s)	741, 795, 941, 1965, 2117
SstI	GAGCTC	1 cut(s)	1465
StyD4I	CCNGG	7 cut(s)	123, 175, 466, 682, 823, 824, 970
TaiI	ACGT	2 cut(s)	788, 1240
TaqII	GACCGA	1 cut(s)	1925
TasI	AATT	8 cut(s)	425, 1162, 1212, 1369, 1506, 1734, 1904, 2309
TatI	WGTACW	4 cut(s)	693, 753, 1024, 1887
TauI	GCSGC	4 cut(s)	168, 361, 1120, 1404
TfiI	GAWTC	5 cut(s)	303, 667, 737, 1557, 2138
TscAI	CASTG	6 cut(s)	279, 313, 888, 984, 1054, 1834
TseFI	GTSAC	1 cut(s)	981
TseI	GCWGC	5 cut(s)	568, 835, 1969, 2144, 2255
Tsp45I	GTSAC	1 cut(s)	981
TspDTI	ATGAA	7 cut(s)	596, 750, 1517, 1637, 1788, 2225, 2226
TspGWI	ACGGA	3 cut(s)	325, 444, 815
TspMI	CCCGGG	1 cut(s)	824
TspRI	CASTG	6 cut(s)	279, 313, 888, 984, 1054, 1834
Van91I	CCANNNNNTGG	1 cut(s)	1049
Vha464I	CTTAAG	1 cut(s)	1352
VneI	GTGCAC	2 cut(s)	1308, 1360
VpaK11BI	GGWCC	4 cut(s)	121, 146, 383, 1133
VspI	ATTAAT	1 cut(s)	707
XagI	CCTNNNNNAGG	1 cut(s)	873
XapI	RAATTY	2 cut(s)	1212, 2309
XceI	RCATGY	5 cut(s)	822, 1031, 1117, 1429, 1844
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1679
XhoI	CTCGAG	1 cut(s)	745
XmaI	CCCGGG	1 cut(s)	824
XmiI	GTMKAC	2 cut(s)	79, 1926
XmnI	GAANNNNTTC	4 cut(s)	65, 548, 725, 2190
XspI	CTAG	5 cut(s)	741, 795, 941, 1965, 2117
ZrmI	AGTACT	2 cut(s)	755, 1889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.