MD04G1183100.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003096
Physical Location & Seq
Reverse (-)
27441450 .. 27447131
5682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1183100.v1.1.491

Sequence Viewer

Length: 2868 bp
ATGCGGCTGCTGCTGCTCTTTCTGGCTTTCTGCTTCTCTGGGATTCATGTGATCTCTTCTTCTACCCACCAAGATGATGCTAGTCGACTCCAATCCTTGAGGCAGACATGGAACAACGTTCCACCCAGCTGGGACAAGTCAAACGATCCATGTGGGGAGCGCTGGGAAGGCATCACTTGCAGCGGTTCGAGGGTGACTGCATTGGGATTATCAGGAATGAACGTGGAAGGGAAAATTGAAGGCGACATCGCGGGGCTCTCTGAGTTGAGATCCTTGGATCTTTCATTCAACAAAGGCCTCACCGGTTCTCTCTCTCCACGATTAGGGGAACTGAGCAATCTAAACACCCTGATCCTAGCTGGTTGCAGCTTCAGTGGAATTATTCCAAGTGAATTGGGGAAACTTGGAAAGCTAACCTTCTTGGCTTTGAATACGAATAACTTCACCGGTAGTATACCTGCTTCTTTGGGTAATCTCTCCAACCTCTATTGGCTGGACCTGGCAGACAATCAGTTGACTGGACCTCTCCCAGTTTCCCCCGGCTTAGACCAACTGTTTAAGGCTAAACATTTCCATTTGAACCAGAACCAGCTTTCAGGTACCATTCCACCCAGACTTTTCAGCTCCAAGATGACACTGATCCATGTATTGTTTGATGGAAATAGATTCAATGGGACTATTCCATCAACAATATTTTCCGTTCAGACTCTCGAGGTTCTTCGGCTTGATAGAAATGCGCTGACGGGACCAGTCTCCTCAGATATCTCCAACCTAATATATCTCAGTGAATTAAATTTCGCGTACAACAATTTGAACGGCTCTTTACCCGACCTAACTGGATTGAATGCCCTCAATTATGTAGACCTTAGTAACAACTCGTTTGATCCATCAGAAGCTCCGCGTTGGATCTCAACCCTGCCCTCGCTCACCACTATCGTTTTAGAAAATGTAGCACTTCAAGGGACTGTGCCAGAGCAGATGTTCAGCATACAGTCATTGCAGCAAGTGAAACTGAAAAACAATGCGTTTAACGACACATTTAACATGGGTGATTACATCAGTCCGCAACTGACGCTTGTTGATCTGCAGAACAATCAGATTCAGAGAATAACCCAGAGTTATAAGTACAAAAATACATTAATACTTGTTGGGAACCCATTGTGCAATGAATCTAGCTCGAGTCCGTACTGTCAGCCTCCACAATCAAATACAAAACCGTATGCTACTAGCACGGCTTGTCCATCCATTACATGCCCCGTGGGTCAGCAGCCAAGCCCTCAGAGTTGTCAATGTGGATATCCTTTTGAAGGAACACTGGACTTCCGAGCGCCCTCCTTCAGCGATTTGGAAAATATGACTCTGTATCAATCCCTAGAAAGGAGCCTATGGGAGAAACTTGACCTCACTCCCGGTTCAGTGTCACTTGATAACCCTTTCTTCGACATAGATGACCATCTTAAAGTACATGTGGCTCTCTTTCCACCCACAGGAACATATTTTAATAGGTCTGAGATTATCAGGATTGGTTTTGATATGAGCAATCAGACTTACAAGCCGCCAAAGGAGTTTGGACCCTTCATTTTTAATGCAGCTCAATATAATTTCCCAGACACGCATAAAAGCTCTATGAGCACTGGTGTTATTGTCGGGATATCAGTTAGTTGTCTCGTTCTTGTTGTGGGCCTCGTGGTAGTAGGAATTTACGCCTTTAGGCAAAAGAAACGTGCAGAAAGAGCCATTGGTTTAAGCAGACCTTTCGCTTCTTGGGTGCCAAGCGGAAATGATAGTGGCGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCGTATGATGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAGTAATGAGATAGGATCTGGCGGCTACGGGAAGGTTTACAGGGGCATGCTTTCTGATGGACAAGTGATAGCAATCAAAAGAGCTCAGCAAGGATCAATGCAGGGAGGCCTCGAGTTCAAGACTGAAATTGAGTTGCTCTCTCGCGTTCATCACAAAAATGTCGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTTTATGCCTAATGGAACACTTAGGGAAGGCTTGTCAGGGAGATCTGGTATTCACCTCGATTGGAAGAGGAGACTCCGAATCACTCTTGGCTCGGCAAGAGGACTGGCTTACCTACATGAGCTTGCAAATCCTCCTATAATTCACAGAGATGTGAAGTCCACCAATATTCTATTAGATGAACATTTAACGGCGAAGGTTGCAGATTTTGGCCTCTCGAAGCTGGTCAATGACAGCGGAAAAGGACATGTCTCGACTCAGGTCAAAGGAACAATGGGCTATCTGGATCCTGAGTACTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCAGCCAATTGAGAAGGGAAAATACATTGTCCGCGAGGTACGATTGGTGATGAACAAGAATGACGAAGAGCATTACGGTTTGAGGGAGTTAATAGATCGAAACATTCGAAACTCAGGAACTCTTATTGGGTTTGGGAGGTTCTTGGAGCTAGCCATGCAATGCGTTGAAGAATCAGCTGCAGATCGTCCCACGATGAGTGAAGTTGTGAAGGCTATCGAAACCATTCTGCAGAACGATGGGATGAACACAAACTCAACATCAGCATCTTCATCAGCCACAGAGTTTGCAGCATCAAAAGGTGCTCCGAAACATCCCTACAACGATGGCTTGCCGAAAAAGGAATTCAATGACAGCACCGGCTCCTTTGATTACAGCGGTGGATACGCTGTTTCAGCGAAAATCGAACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase_Tyr

Protein Analysis

956

Amino Acids

105.05

Weight (kDa)

6.15

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 61 1.1e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 81 - 169 7.3e-06 Leucine-rich repeat region
Pkinase PF00069 621 - 888 2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 624 - 889 2.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1122
Acc36I ACCTGC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 599
AccB1I GGYRCC 2 cut(s) 599, 1768
AccI GTMKAC 4 cut(s) 85, 454, 861, 2412
AccII CGCG 5 cut(s) 251, 800, 901, 2010, 2487
AclI AACGTT 1 cut(s) 117
AcsI RAATTY 3 cut(s) 793, 1698, 2795
AcuI CTGAAG 2 cut(s) 355, 1321
AfaI GTAC 7 cut(s) 601, 803, 1127, 1187, 1464, 2375, 2493
AfeI AGCGCT 1 cut(s) 161
AfiI CCNNNNNNNGG 4 cut(s) 323, 1307, 1377, 1487
AflII CTTAAG 1 cut(s) 1838
AflIII ACRYGT 2 cut(s) 1465, 2326
AgeI ACCGGT 2 cut(s) 302, 446
AjnI CCWGG 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 1722, 1754
Alw21I GWGCWC 5 cut(s) 1634, 1798, 1850, 1951, 2758
Alw26I GTCTC 4 cut(s) 757, 1670, 2146, 2335
Alw44I GTGCAC 2 cut(s) 1794, 1846
AlwNI CAGNNNCTG 1 cut(s) 2071
Ama87I CYCGRG 3 cut(s) 710, 1177, 1976
Aor51HI AGCGCT 1 cut(s) 161
AoxI GGCC 4 cut(s) 295, 1681, 1972, 2290
ApaLI GTGCAC 2 cut(s) 1794, 1846
ApoI RAATTY 3 cut(s) 793, 1698, 2795
AseI ATTAAT 1 cut(s) 1139
AsiGI ACCGGT 2 cut(s) 302, 446
Asp700I GAANNNNTTC 2 cut(s) 440, 2676
Asp718I GGTACC 1 cut(s) 599
AspLEI GCGC 3 cut(s) 162, 739, 1330
AspS9I GGNCC 5 cut(s) 496, 521, 746, 1571, 1681
AsuC2I CCSGG 2 cut(s) 540, 1410
AsuHPI GGTGA 7 cut(s) 205, 292, 436, 919, 1061, 2126, 2512
AsuII TTCGAA 2 cut(s) 2052, 2560
AsuNHI GCTAGC 1 cut(s) 2602
AvaI CYCGRG 3 cut(s) 710, 1177, 1976
AvaII GGWCC 4 cut(s) 496, 521, 746, 1571
BaeGI GKGCMC 2 cut(s) 1798, 1850
BamHI GGATCC 1 cut(s) 2365
BanI GGYRCC 2 cut(s) 599, 1768
BanII GRGCYC 2 cut(s) 258, 1951
BarI GAAGNNNNNNTAC 4 cut(s) 445, 477, 2459, 2491
BauI CACGAG 1 cut(s) 1685
Bbv12I GWGCWC 5 cut(s) 1634, 1798, 1850, 1951, 2758
BbvI GCAGC 8 cut(s) 192, 378, 1012, 1279, 1601, 2467, 2617, 2753
BccI CCATC 9 cut(s) 650, 691, 895, 1249, 1461, 1812, 1916, 2684, 2771
BceAI ACGGC 3 cut(s) 832, 1248, 2286
BcgI CGANNNNNNTGC 2 cut(s) 1738, 1772
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 2828
BcnI CCSGG 2 cut(s) 540, 1410
BcoDI GTCTC 4 cut(s) 757, 1670, 2146, 2335
BfaI CTAG 7 cut(s) 81, 356, 1173, 1227, 1373, 2451, 2603
BfmI CTRYAG 3 cut(s) 1085, 2631, 2681
BfoI RGCGCY 2 cut(s) 163, 1331
BfrI CTTAAG 1 cut(s) 1838
BfuAI ACCTGC 1 cut(s) 466
BfuI GTATCC 1 cut(s) 2828
BglII AGATCT 1 cut(s) 2123
BlpI GCTNAGC 1 cut(s) 1950
BmcAI AGTACT 1 cut(s) 2375
Bme1390I CCNGG 3 cut(s) 500, 540, 1410
Bme18I GGWCC 4 cut(s) 496, 521, 746, 1571
BmeT110I CYCGRG 3 cut(s) 710, 1177, 1976
BmgT120I GGNCC 5 cut(s) 496, 521, 746, 1571, 1681
BmiI GGNNCC 8 cut(s) 601, 747, 1154, 1382, 1573, 1770, 2367, 2815
BmrFI CCNGG 3 cut(s) 500, 540, 1410
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 5 cut(s) 67, 180, 2058, 2726, 2753
BmtI GCTAGC 1 cut(s) 2606
BmuI ACTGGG 1 cut(s) 524
BoxI GACNNNNGTC 1 cut(s) 2339
BplI GAGNNNNNCTC 2 cut(s) 1988, 2020
Bpu1102I GCTNAGC 1 cut(s) 1950
Bpu14I TTCGAA 2 cut(s) 2052, 2560
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 2 cut(s) 540, 1410
BsaBI GATNNNNATC 2 cut(s) 1452, 1937
BsaJI CCNNGG 3 cut(s) 273, 538, 1257
BsaWI WCCGGW 2 cut(s) 302, 446
BsaXI ACNNNNNCTCC 2 cut(s) 2113, 2143
Bsc4I CCNNNNNNNGG 4 cut(s) 323, 1307, 1377, 1487
Bse118I RCCGGY 3 cut(s) 302, 446, 2810
Bse1I ACTGG 7 cut(s) 523, 530, 749, 841, 1320, 1639, 2190
Bse3DI GCAATG 3 cut(s) 995, 1171, 2618
Bse8I GATNNNNATC 2 cut(s) 1452, 1937
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 3 cut(s) 273, 538, 1257
BseGI GGATG 3 cut(s) 1241, 2700, 2764
BseJI GATNNNNATC 2 cut(s) 1452, 1937
BseLI CCNNNNNNNGG 4 cut(s) 323, 1307, 1377, 1487
BseMI GCAATG 3 cut(s) 995, 1171, 2618
BseNI ACTGG 7 cut(s) 523, 530, 749, 841, 1320, 1639, 2190
BseRI GAGGAG 2 cut(s) 745, 2164
BseSI GKGCMC 2 cut(s) 1798, 1850
BseXI GCAGC 8 cut(s) 192, 378, 1012, 1279, 1601, 2467, 2617, 2753
BseYI CCCAGC 3 cut(s) 125, 129, 162
BsgI GTGCAG 1 cut(s) 1746
Bsh1236I CGCG 5 cut(s) 251, 800, 901, 2010, 2487
BshFI GGCC 4 cut(s) 297, 1683, 1974, 2292
BshNI GGYRCC 2 cut(s) 599, 1768
BshTI ACCGGT 2 cut(s) 302, 446
BsiHKAI GWGCWC 5 cut(s) 1634, 1798, 1850, 1951, 2758
BsiHKCI CYCGRG 3 cut(s) 710, 1177, 1976
BsiSI CCGG 5 cut(s) 303, 447, 540, 1410, 2811
BslFI GGGAC 5 cut(s) 146, 688, 759, 976, 2625
BslI CCNNNNNNNGG 4 cut(s) 323, 1307, 1377, 1487
BsmAI GTCTC 4 cut(s) 757, 1670, 2146, 2335
BsmFI GGGAC 5 cut(s) 146, 688, 759, 976, 2625
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 4 cut(s) 297, 1683, 1974, 2292
BsoBI CYCGRG 3 cut(s) 710, 1177, 1976
Bsp119I TTCGAA 2 cut(s) 2052, 2560
Bsp1286I GDGCHC 6 cut(s) 258, 1634, 1798, 1850, 1951, 2758
Bsp1720I GCTNAGC 1 cut(s) 1950
BspANI GGCC 4 cut(s) 297, 1683, 1974, 2292
BspFNI CGCG 5 cut(s) 251, 800, 901, 2010, 2487
BspLI GGNNCC 8 cut(s) 601, 747, 1154, 1382, 1573, 1770, 2367, 2815
BspMAI CTGCAG 3 cut(s) 1089, 2635, 2685
BspMI ACCTGC 1 cut(s) 466
BspOI GCTAGC 1 cut(s) 2606
BspQI GCTCTTC 2 cut(s) 2396, 2514
BspT104I TTCGAA 2 cut(s) 2052, 2560
BspT107I GGYRCC 2 cut(s) 599, 1768
BspTI CTTAAG 1 cut(s) 1838
BsrDI GCAATG 3 cut(s) 995, 1171, 2618
BsrFI RCCGGY 3 cut(s) 302, 446, 2810
BsrI ACTGG 7 cut(s) 523, 530, 749, 841, 1320, 1639, 2190
BssAI RCCGGY 3 cut(s) 302, 446, 2810
BssECI CCNNGG 3 cut(s) 273, 538, 1257
BssNAI GTATAC 2 cut(s) 455, 2413
BssSI CACGAG 1 cut(s) 1685
BssT1I CCWWGG 1 cut(s) 273
Bst1107I GTATAC 2 cut(s) 455, 2413
Bst2BI CACGAG 1 cut(s) 1685
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 8 cut(s) 555, 967, 993, 1190, 1218, 1803, 1868, 2531
Bst6I CTCTTC 4 cut(s) 61, 2141, 2396, 2514
BstAFI CTTAAG 1 cut(s) 1838
BstAPI GCANNNNNTGC 1 cut(s) 177
BstBI TTCGAA 2 cut(s) 2052, 2560
BstC8I GCNNGC 4 cut(s) 1913, 2205, 2604, 2783
BstDSI CCRYGG 1 cut(s) 1257
BstENI CCTNNNNNAGG 1 cut(s) 1305
BstF5I GGATG 3 cut(s) 1241, 2700, 2764
BstFNI CGCG 5 cut(s) 251, 800, 901, 2010, 2487
BstH2I RGCGCY 2 cut(s) 163, 1331
BstHHI GCGC 3 cut(s) 162, 739, 1330
BstMAI GTCTC 4 cut(s) 757, 1670, 2146, 2335
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 4 cut(s) 1254, 1469, 1915, 2330
BstPAI GACNNNNGTC 1 cut(s) 2339
BstSCI CCNGG 3 cut(s) 498, 538, 1408
BstSFI CTRYAG 3 cut(s) 1085, 2631, 2681
BstSLI GKGCMC 2 cut(s) 1798, 1850
BstUI CGCG 5 cut(s) 251, 800, 901, 2010, 2487
BstV1I GCAGC 8 cut(s) 192, 378, 1012, 1279, 1601, 2467, 2617, 2753
BstX2I RGATCY 6 cut(s) 269, 277, 906, 1880, 2123, 2365
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 6 cut(s) 269, 277, 906, 1880, 2123, 2365
BstZ17I GTATAC 2 cut(s) 455, 2413
BsuI GTATCC 1 cut(s) 2828
BsuRI GGCC 4 cut(s) 297, 1683, 1974, 2292
BtgI CCRYGG 1 cut(s) 1257
BtgZI GCGATG 2 cut(s) 232, 2421
BtsCI GGATG 3 cut(s) 1241, 2700, 2764
BtsIMutI CAGTG 6 cut(s) 379, 635, 790, 1313, 1422, 1632
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 4 cut(s) 1913, 2205, 2604, 2783
CaiI CAGNNNCTG 1 cut(s) 2071
CfoI GCGC 3 cut(s) 162, 739, 1330
Cfr10I RCCGGY 3 cut(s) 302, 446, 2810
Cfr13I GGNCC 5 cut(s) 496, 521, 746, 1571, 1681
CseI GACGC 1 cut(s) 1081
Csp6I GTAC 7 cut(s) 600, 802, 1126, 1186, 1463, 2374, 2492
CspAI ACCGGT 2 cut(s) 302, 446
CspCI CAANNNNNGTGG 2 cut(s) 2719, 2754
CviQI GTAC 7 cut(s) 600, 802, 1126, 1186, 1463, 2374, 2492
Eam1104I CTCTTC 4 cut(s) 61, 2141, 2396, 2514
EarI CTCTTC 4 cut(s) 61, 2141, 2396, 2514
Ecl136II GAGCTC 1 cut(s) 1949
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 2 cut(s) 297, 1974
Eco24I GRGCYC 2 cut(s) 258, 1951
Eco32I GATATC 3 cut(s) 763, 1298, 1653
Eco47I GGWCC 4 cut(s) 496, 521, 746, 1571
Eco47III AGCGCT 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 1949
Eco57I CTGAAG 2 cut(s) 355, 1321
Eco88I CYCGRG 3 cut(s) 710, 1177, 1976
EcoICRI GAGCTC 1 cut(s) 1949
EcoNI CCTNNNNNAGG 1 cut(s) 1305
EcoRI GAATTC 1 cut(s) 2795
EcoRII CCWGG 1 cut(s) 498
EcoRV GATATC 3 cut(s) 763, 1298, 1653
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 258, 1951
ErhI CCWWGG 1 cut(s) 273
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 1738, 1770
FaqI GGGAC 5 cut(s) 146, 688, 759, 976, 2625
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 4 cut(s) 85, 454, 861, 2412
FokI GGATG 3 cut(s) 1228, 2707, 2751
FriOI GRGCYC 2 cut(s) 258, 1951
FspBI CTAG 7 cut(s) 81, 356, 1173, 1227, 1373, 2451, 2603
GlaI GCGC 3 cut(s) 161, 738, 1329
GsaI CCCAGC 3 cut(s) 129, 133, 166
HaeII RGCGCY 2 cut(s) 163, 1331
HaeIII GGCC 4 cut(s) 297, 1683, 1974, 2292
HapII CCGG 5 cut(s) 303, 447, 540, 1410, 2811
HgaI GACGC 1 cut(s) 1081
HhaI GCGC 3 cut(s) 162, 739, 1330
Hin6I GCGC 3 cut(s) 160, 737, 1328
HinP1I GCGC 3 cut(s) 160, 737, 1328
HincII GTYRAC 2 cut(s) 86, 516
HindII GTYRAC 2 cut(s) 86, 516
HpaII CCGG 5 cut(s) 303, 447, 540, 1410, 2811
HphI GGTGA 7 cut(s) 205, 292, 436, 919, 1061, 2126, 2512
HpyCH4III ACNGT 8 cut(s) 555, 967, 993, 1190, 1218, 1803, 1868, 2531
HpyCH4IV ACGT 3 cut(s) 117, 222, 1723
HpySE526I ACGT 3 cut(s) 117, 222, 1723
HspAI GCGC 3 cut(s) 160, 737, 1328
KpnI GGTACC 1 cut(s) 603
LguI GCTCTTC 2 cut(s) 2396, 2514
LmnI GCTCC 8 cut(s) 157, 629, 901, 1380, 1811, 2599, 2761, 2819
Lsp1109I GCAGC 8 cut(s) 192, 378, 1012, 1279, 1601, 2467, 2617, 2753
LweI GCATC 5 cut(s) 67, 180, 2058, 2726, 2753
MaeI CTAG 7 cut(s) 81, 356, 1173, 1227, 1373, 2451, 2603
MaeII ACGT 3 cut(s) 117, 222, 1723
MaeIII GTNAC 3 cut(s) 193, 869, 1419
MboII GAAGA 9 cut(s) 48, 51, 710, 1429, 2158, 2413, 2531, 2633, 2712
MfeI CAATTG 2 cut(s) 2390, 2460
MflI RGATCY 6 cut(s) 269, 277, 906, 1880, 2123, 2365
MhlI GDGCHC 6 cut(s) 258, 1634, 1798, 1850, 1951, 2758
MlyI GAGTC 8 cut(s) 81, 700, 1189, 1351, 2148, 2329, 2377, 2433
MmeI TCCRAC 3 cut(s) 504, 792, 884
MroXI GAANNNNTTC 2 cut(s) 440, 2676
MslI CAYNNNNRTG 3 cut(s) 72, 2022, 2229
MspA1I CMGCKG 5 cut(s) 129, 183, 2316, 2630, 2829
MspCI CTTAAG 1 cut(s) 1838
MspI CCGG 5 cut(s) 303, 447, 540, 1410, 2811
MspR9I CCNGG 3 cut(s) 500, 540, 1410
MunI CAATTG 2 cut(s) 2390, 2460
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 5 cut(s) 251, 800, 901, 2010, 2487
NciI CCSGG 2 cut(s) 540, 1410
NheI GCTAGC 1 cut(s) 2602
NlaIV GGNNCC 8 cut(s) 601, 747, 1154, 1382, 1573, 1770, 2367, 2815
NmeAIII GCCGAG 1 cut(s) 2153
NmuCI GTSAC 2 cut(s) 193, 1419
NspI RCATGY 4 cut(s) 1254, 1469, 1915, 2330
NspV TTCGAA 2 cut(s) 2052, 2560
PaeI GCATGC 1 cut(s) 1915
PaeR7I CTCGAG 3 cut(s) 710, 1177, 1976
PceI AGGCCT 2 cut(s) 297, 1974
PciI ACATGT 2 cut(s) 1465, 2326
PciSI GCTCTTC 2 cut(s) 2396, 2514
PcsI WCGNNNNNNNCGW 1 cut(s) 2557
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 2 cut(s) 440, 2676
PfeI GAWTC 7 cut(s) 43, 666, 1099, 1169, 2043, 2160, 2624
PinAI ACCGGT 2 cut(s) 302, 446
PleI GAGTC 8 cut(s) 81, 700, 1188, 1351, 2148, 2329, 2377, 2432
PpsI GAGTC 8 cut(s) 81, 700, 1188, 1351, 2148, 2329, 2377, 2432
PscI ACATGT 2 cut(s) 1465, 2326
PshAI GACNNNNGTC 1 cut(s) 2339
PshBI ATTAAT 1 cut(s) 1139
PsiI TTATAA 1 cut(s) 1122
Psp124BI GAGCTC 1 cut(s) 1951
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 3 cut(s) 125, 129, 162
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 8 cut(s) 601, 747, 1154, 1382, 1573, 1770, 2367, 2815
PspPI GGNCC 5 cut(s) 496, 521, 746, 1571, 1681
PspXI VCTCGAGB 2 cut(s) 1177, 1976
PstI CTGCAG 3 cut(s) 1089, 2635, 2685
PstNI CAGNNNCTG 1 cut(s) 2071
PsuI RGATCY 6 cut(s) 269, 277, 906, 1880, 2123, 2365
PvuII CAGCTG 2 cut(s) 129, 2630
RsaI GTAC 7 cut(s) 601, 803, 1127, 1187, 1464, 2375, 2493
RsaNI GTAC 7 cut(s) 600, 802, 1126, 1186, 1463, 2374, 2492
RseI CAYNNNNRTG 3 cut(s) 72, 2022, 2229
SacI GAGCTC 1 cut(s) 1951
SalI GTCGAC 1 cut(s) 84
SapI GCTCTTC 2 cut(s) 2396, 2514
Sau96I GGNCC 5 cut(s) 496, 521, 746, 1571, 1681
ScaI AGTACT 1 cut(s) 2375
SchI GAGTC 8 cut(s) 81, 700, 1189, 1351, 2148, 2329, 2377, 2433
ScrFI CCNGG 3 cut(s) 500, 540, 1410
SduI GDGCHC 6 cut(s) 258, 1634, 1798, 1850, 1951, 2758
SfaNI GCATC 5 cut(s) 67, 180, 2058, 2726, 2753
SfcI CTRYAG 3 cut(s) 1085, 2631, 2681
Sfr274I CTCGAG 3 cut(s) 710, 1177, 1976
SfuI TTCGAA 2 cut(s) 2052, 2560
SinI GGWCC 4 cut(s) 496, 521, 746, 1571
SlaI CTCGAG 3 cut(s) 710, 1177, 1976
SmiMI CAYNNNNRTG 3 cut(s) 72, 2022, 2229
SmlI CTYRAG 5 cut(s) 97, 710, 1177, 1838, 1976
SmoI CTYRAG 5 cut(s) 97, 710, 1177, 1838, 1976
SphI GCATGC 1 cut(s) 1915
SseBI AGGCCT 2 cut(s) 297, 1974
SspI AATATT 2 cut(s) 693, 2248
SspMI CTAG 7 cut(s) 81, 356, 1173, 1227, 1373, 2451, 2603
SstI GAGCTC 1 cut(s) 1951
StuI AGGCCT 2 cut(s) 297, 1974
StyD4I CCNGG 3 cut(s) 498, 538, 1408
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 8 cut(s) 555, 967, 993, 1190, 1218, 1803, 1868, 2531
TaiI ACGT 3 cut(s) 120, 225, 1726
TaqII GACCGA 1 cut(s) 2411
TatI WGTACW 3 cut(s) 1125, 1462, 2373
TauI GCSGC 3 cut(s) 7, 1558, 1890
TfiI GAWTC 7 cut(s) 43, 666, 1099, 1169, 2043, 2160, 2624
TscAI CASTG 6 cut(s) 379, 642, 790, 1320, 1422, 1639
TseFI GTSAC 2 cut(s) 193, 1419
Tsp45I GTSAC 2 cut(s) 193, 1419
TspGWI ACGGA 2 cut(s) 688, 1173
TspRI CASTG 6 cut(s) 379, 642, 790, 1320, 1422, 1639
Vha464I CTTAAG 1 cut(s) 1838
VneI GTGCAC 2 cut(s) 1794, 1846
VpaK11BI GGWCC 4 cut(s) 496, 521, 746, 1571
VspI ATTAAT 1 cut(s) 1139
XagI CCTNNNNNAGG 1 cut(s) 1305
XapI RAATTY 3 cut(s) 793, 1698, 2795
XceI RCATGY 4 cut(s) 1254, 1469, 1915, 2330
XhoI CTCGAG 3 cut(s) 710, 1177, 1976
XmiI GTMKAC 4 cut(s) 85, 454, 861, 2412
XmnI GAANNNNTTC 2 cut(s) 440, 2676
XspI CTAG 7 cut(s) 81, 356, 1173, 1227, 1373, 2451, 2603
ZrmI AGTACT 1 cut(s) 2375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.