Rw3G007970

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003096
Physical Location & Seq
Forward (+)
7407776 .. 7413676
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007970.1

Sequence Viewer

Length: 2775 bp
ATGGCTTTTGTTTATGGTGCAAGTTGTATAAGAAGGGCTTCTTTGGGTGCTGCTGGGCTTCAATCCTTCAAGGCCGTGTGGCGAAATTATCCACCCAGCTGGGACCGGTCAAGTGATTACTGTGGGTGGGAAGGAATCACCTGCAATCAGGAATCCAGTGTGACTGCAATGAGATTATCAGGCATGGGCCTGGTAGGGGATCTTAGTGGTGACATTGGGGCATTTACTGAATTAACATCCTTGGACCTTTCATTCAACCCTGGACTTACTGGTCCTCTCTCTCCACAATTAGGGCAGCTGCAAAAGTTAAATATTCTGATCCTAGCTGGCTGCAAATTCACTGGCAGTATTCCAGATGAATTGGGAAATCTTGGAGAGCTAACCTTCTTGGCTCTGAATACAAACAATTTGACCGGTAAAATACCTGCTTCTTTGGGTAAACTCTCCAAACTCTACTGGCTGGACCTTGCAGAAAATCAGTTGACTGGACCTATCCCAGTTTCATCCGCAACATCCCCAGGCTTGGACCAACTAGTGAAAGCTAAACATTTCCATTTCAACAAGAACCAGCTTTCAGGTTCAATTCCAGCCACACTATTCAGCGCTGAGATGGTCCTGATACATGTGTTGTTTGATGGAAACAAATTAAGTGGGACTATTCCATCAACAATAGGACAAGTTCAGACTCTTGAGGCTCTTCGGCTTGATAGAAATACTCTGACGGGACCAGTCCCAAAAAGTATTAACAACCTTACAAATGTCAATGAATTAAATTTGGCCCTTAACAATTTGACAGGCCCTCTGCCCGACTTGACTGGAATGAACTCCCTCAATTATTTAGACCTTAGTAACAACTCATTTGATCCATCAGAAGCACCAAGTTGGTTCTCAGCTTTACCCGTACTCACCACTCTGGTTGTAGAATATGGACCACTTCAAGGTACTGTGCCAGAGAAGCTTTTCAGCCTACAAGATCTACAGCAAGTGAAACTGAAAAATAATGCATTTAACGACACACTGAACTTGGGTGATAGTATCGGTGCACAACTAAATGTTGTTGATCTGCAGAACAATGATATTTCCCAAGTCACTATAGGTTATGAGTACAAAAATTCATTAATACTTGTAGGGAACCCTGTGTGCAACACAACCACTGGTCTCCCTTATTGTCAGATTCCGCAAACTACAGTATATTCTACCACCATTAGTTGTGGAAACGCTAAATGCCCCGAGGATCAGAAGCTCAGCCCTCAGAGTTGTCAGTGTGCATATCCGTTTGAAGGAACACTGTACTTTAGAGCACCCACTTTCAGGGATTTATCAGATGCAAATAAGTTTCACTCCCTAGAAATGAGCCTGTGGTCGAAACTTGGCCTCAATCCTGGTTCAGTTGCTCTTGAGAACCCTTTCTTCAACATTGATGACTATCTTCAAATACAGTTGGCACTCTTTCCATCTATGGGAACCTATTTTAATAGGTCAGAGATTCTGAGGATTGGTTTTGATCTGAGCAATCAAACTTACAAGCCGCCTGCTGAGTTTGGTCCCTATATTTTTATTCCAGCTCCTTATACTTTCCGGGCCGCGCATAAGAAATCTGTAAGCACTGGTGTTGTCATTGGGATAGCAATTGGTTGTACCTTTCTGGTTTTGGGCTTAGTGGGAGTCGGAATATATGCCATTCGGCAAAAAAAACGTGCAGAAAGAGCCATTGGATTAAGCAGACCATTTGCGTCTTGGGCACCCAGCGGAAAAGATAGTGGGGGTGCACCACAGTTAAAGGGAGCAAGATGGTTTTCTTATGAGGAGCTTAAGAAGTGCACCAATAATTTCTCTGACAATAATGAGATAGGATCCGGCGGCTACGGCAAGGTTTACAGGGGCATGTTTGCTGATGGACTAGTTGTGGCAATCAAAAGAGCTCAGCAAGGATCAATGCAGGGTGGTCTTGAGTTCAAGACTGAAATTGAATTGCTCTCACGAGTTCATCACAAAAATCTTGTCGGTCTTGTCGGGTTTTGTTTTGAACAAGGGAACAGATGCTGGTTTACGAATATATGCCCAATGGAACACTTAGGGAAAGCTTGTCAGGTCAGGAGATCTGGCATTCATCTCGACTGGAAGCGAAGACTCCGCATCACTCTTGGCTCAGCAAGAGGACTAGCATACCTACATGAGCTTGCAAACCCTCCGATAATCCATAGAGATATTAAAACCACCAATATTCTATTGGATGAACATTTAACGGCAAAGGTTGCAGATTTTGGCTTGTCCAAGCTGGTTTCTGACAGTGCAAAAGGACATGTTTCAACTCAGGTCAAAGGCACACTGGGATATCTGGATCCTGAATACTACATGACTCAACAGTTAACTGAGAAGAGTGATGTATACAGTTTCGGAGTGGTTATGCTTGAATTGATAACTGCAAAGCAACCGATTGAGAAGGGCAAATACATTGTCCGCGAGGTACGCATGATAATGAACAAGGATGATGAAGAGTACTATGGGTTGGGGGAGTTAATAGATCGAAACATTAGAAACTCAGGACCCCTTATAGGTTTTGCGAGGTTCTTGGAGTTGGCCATGCAATGCGTTGAAGAATCAGCTGCAGATCGTCCAACAATGAGTGATCTTGTCAAGGCTGTTGAAACCATTCTACAGAATGATGGGATGAATACAAACTCAACATCAGCCTCTTCATCTGCCACTGACTTTAATTCTTCAAAAGTGCTCCTAAACATCCATACAACGACGGTTTGCCAAAGAAGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

924

Amino Acids

101.16

Weight (kDa)

6.91

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 19 - 49 4.5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 229 - 286 5.5e-06 Leucine rich repeat
Pkinase PF00069 612 - 880 7.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 614 - 880 7e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
AasI GACNNNNNNGTC 1 cut(s) 270
Acc36I ACCTGC 2 cut(s) 149, 433
AccB1I GGYRCC 1 cut(s) 1741
AccI GTMKAC 1 cut(s) 2388
AccII CGCG 2 cut(s) 1586, 2463
AciI CCGC 8 cut(s) 507, 1178, 1529, 1584, 1749, 1860, 2134, 2461
AclWI GGATC 9 cut(s) 207, 313, 857, 1242, 1848, 1861, 1939, 2336, 2349
AcoI YGGCCR 1 cut(s) 2580
AcsI RAATTY 3 cut(s) 335, 772, 1111
AfaI GTAC 7 cut(s) 903, 943, 1106, 1292, 1639, 2469, 2501
AfeI AGCGCT 1 cut(s) 604
AfiI CCNNNNNNNGG 8 cut(s) 196, 290, 523, 938, 1280, 1311, 1460, 2555
AflII CTTAAG 1 cut(s) 1811
AflIII ACRYGT 2 cut(s) 622, 2302
AgeI ACCGGT 2 cut(s) 105, 413
AhlI ACTAGT 2 cut(s) 532, 1900
AjnI CCWGG 4 cut(s) 189, 259, 517, 1381
AjuI GAANNNNNNNTTGG 2 cut(s) 1695, 1727
Alw21I GWGCWC 6 cut(s) 1045, 1303, 1771, 1823, 1924, 2733
Alw26I GTCTC 1 cut(s) 1163
Alw44I GTGCAC 3 cut(s) 1041, 1767, 1819
AlwI GGATC 9 cut(s) 207, 313, 857, 1242, 1848, 1861, 1939, 2336, 2349
AlwNI CAGNNNCTG 1 cut(s) 2043
Ama87I CYCGRG 1 cut(s) 1229
Aor51HI AGCGCT 1 cut(s) 604
AoxI GGCC 7 cut(s) 72, 187, 777, 796, 1372, 1581, 2580
ApaLI GTGCAC 3 cut(s) 1041, 1767, 1819
ApeKI GCWGC 5 cut(s) 50, 295, 298, 330, 2606
ApoI RAATTY 3 cut(s) 335, 772, 1111
ArsI GACNNNNNNTTYG 4 cut(s) 1986, 2018, 2442, 2474
AseI ATTAAT 1 cut(s) 1118
AsiGI ACCGGT 2 cut(s) 105, 413
Asp700I GAANNNNTTC 4 cut(s) 37, 959, 1406, 2652
AspLEI GCGC 2 cut(s) 605, 1588
AsuC2I CCSGG 1 cut(s) 1580
AsuHPI GGTGA 4 cut(s) 130, 221, 898, 1040
AvaI CYCGRG 1 cut(s) 1229
BaeGI GKGCMC 4 cut(s) 1045, 1744, 1771, 1823
BaeI ACNNNNGTAYC 2 cut(s) 611, 644
BalI TGGCCA 1 cut(s) 2582
BamHI GGATCC 2 cut(s) 1853, 2341
BanI GGYRCC 1 cut(s) 1741
BanII GRGCYC 1 cut(s) 1924
BarI GAAGNNNNNNTAC 2 cut(s) 2435, 2467
BauI CACGAG 1 cut(s) 1980
BbsI GAAGAC 1 cut(s) 2134
Bbv12I GWGCWC 6 cut(s) 1045, 1303, 1771, 1823, 1924, 2733
BbvI GCAGC 5 cut(s) 37, 285, 307, 317, 2593
BccI CCATC 8 cut(s) 604, 629, 670, 874, 1462, 1785, 1889, 2660
BceAI ACGGC 3 cut(s) 59, 1882, 2262
BcgI CGANNNNNNTGC 2 cut(s) 2095, 2129
BciT130I CCWGG 4 cut(s) 191, 261, 519, 1383
BcnI CCSGG 1 cut(s) 1580
BcoDI GTCTC 1 cut(s) 1163
BcuI ACTAGT 2 cut(s) 532, 1900
BfaI CTAG 5 cut(s) 323, 533, 1346, 1901, 2162
BfmI CTRYAG 6 cut(s) 977, 1064, 1092, 1185, 2607, 2657
BfoI RGCGCY 1 cut(s) 606
BfrI CTTAAG 1 cut(s) 1811
BfuAI ACCTGC 2 cut(s) 149, 433
BglII AGATCT 2 cut(s) 973, 2099
BisI GCNGC 8 cut(s) 51, 296, 299, 331, 1529, 1584, 1861, 2607
BlpI GCTNAGC 3 cut(s) 1244, 1923, 2149
BlsI GCNGC 8 cut(s) 52, 297, 300, 332, 1530, 1585, 1862, 2608
BmcAI AGTACT 1 cut(s) 2501
Bme1390I CCNGG 5 cut(s) 191, 261, 519, 1383, 1580
BmeT110I CYCGRG 1 cut(s) 1229
BmiI GGNNCC 9 cut(s) 104, 726, 1133, 1465, 1546, 1743, 1855, 2343, 2548
BmrFI CCNGG 5 cut(s) 191, 261, 519, 1383, 1580
BmrI ACTGGG 2 cut(s) 491, 2339
BmsI GCATC 3 cut(s) 1315, 2030, 2145
BmuI ACTGGG 2 cut(s) 491, 2339
BpiI GAAGAC 1 cut(s) 2134
Bpu1102I GCTNAGC 3 cut(s) 1244, 1923, 2149
BpuEI CTTGAG 3 cut(s) 710, 1418, 1970
BpuMI CCSGG 1 cut(s) 1580
BsaBI GATNNNNATC 1 cut(s) 1425
BsaI GGTCTC 1 cut(s) 1163
BsaJI CCNNGG 4 cut(s) 240, 259, 517, 1230
BsaWI WCCGGW 2 cut(s) 105, 413
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 290, 523, 938, 1280, 1311, 1460, 2555
Bse118I RCCGGY 2 cut(s) 105, 413
Bse3DI GCAATG 2 cut(s) 174, 2594
Bse8I GATNNNNATC 1 cut(s) 1425
BseBI CCWGG 4 cut(s) 191, 261, 519, 1383
BseDI CCNNGG 4 cut(s) 240, 259, 517, 1230
BseGI GGATG 8 cut(s) 236, 503, 512, 2239, 2494, 2676, 2739, 2775
BseJI GATNNNNATC 1 cut(s) 1425
BseLI CCNNNNNNNGG 8 cut(s) 196, 290, 523, 938, 1280, 1311, 1460, 2555
BseMI GCAATG 2 cut(s) 174, 2594
BseRI GAGGAG 1 cut(s) 1820
BseSI GKGCMC 4 cut(s) 1045, 1744, 1771, 1823
BseXI GCAGC 5 cut(s) 37, 285, 307, 317, 2593
BseYI CCCAGC 4 cut(s) 53, 95, 99, 1745
BsgI GTGCAG 1 cut(s) 1719
Bsh1236I CGCG 2 cut(s) 1586, 2463
BshFI GGCC 7 cut(s) 74, 189, 779, 798, 1374, 1583, 2582
BshNI GGYRCC 1 cut(s) 1741
BshTI ACCGGT 2 cut(s) 105, 413
BsiHKAI GWGCWC 6 cut(s) 1045, 1303, 1771, 1823, 1924, 2733
BsiHKCI CYCGRG 1 cut(s) 1229
BsiSI CCGG 4 cut(s) 106, 414, 1579, 1857
BslFI GGGAC 5 cut(s) 116, 667, 716, 738, 1530
BslI CCNNNNNNNGG 8 cut(s) 196, 290, 523, 938, 1280, 1311, 1460, 2555
BsmAI GTCTC 1 cut(s) 1163
BsmFI GGGAC 5 cut(s) 116, 667, 716, 738, 1530
BsmI GAATGC 1 cut(s) 2106
BsnI GGCC 7 cut(s) 74, 189, 779, 798, 1374, 1583, 2582
Bso31I GGTCTC 1 cut(s) 1163
BsoBI CYCGRG 1 cut(s) 1229
Bsp1286I GDGCHC 7 cut(s) 1045, 1303, 1744, 1771, 1823, 1924, 2733
Bsp1720I GCTNAGC 3 cut(s) 1244, 1923, 2149
BspACI CCGC 8 cut(s) 507, 1178, 1529, 1584, 1749, 1860, 2134, 2461
BspANI GGCC 7 cut(s) 74, 189, 779, 798, 1374, 1583, 2582
BspFNI CGCG 2 cut(s) 1586, 2463
BspLI GGNNCC 9 cut(s) 104, 726, 1133, 1465, 1546, 1743, 1855, 2343, 2548
BspMAI CTGCAG 2 cut(s) 1068, 2611
BspMI ACCTGC 2 cut(s) 149, 433
BspPI GGATC 9 cut(s) 207, 313, 857, 1242, 1848, 1861, 1939, 2336, 2349
BspQI GCTCTTC 1 cut(s) 702
BspT107I GGYRCC 1 cut(s) 1741
BspTI CTTAAG 1 cut(s) 1811
BspTNI GGTCTC 1 cut(s) 1163
BsrDI GCAATG 2 cut(s) 174, 2594
BsrFI RCCGGY 2 cut(s) 105, 413
BssAI RCCGGY 2 cut(s) 105, 413
BssECI CCNNGG 4 cut(s) 240, 259, 517, 1230
BssNAI GTATAC 1 cut(s) 2389
BssSI CACGAG 1 cut(s) 1980
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 2389
Bst2BI CACGAG 1 cut(s) 1980
Bst2UI CCWGG 4 cut(s) 191, 261, 519, 1383
Bst6I CTCTTC 4 cut(s) 702, 2372, 2490, 2701
BstAFI CTTAAG 1 cut(s) 1811
BstAPI GCANNNNNTGC 1 cut(s) 2255
BstC8I GCNNGC 3 cut(s) 328, 1533, 2181
BstF5I GGATG 8 cut(s) 236, 503, 512, 2239, 2494, 2676, 2739, 2775
BstFNI CGCG 2 cut(s) 1586, 2463
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 2 cut(s) 605, 1588
BstMAI GTCTC 1 cut(s) 1163
BstMWI GCNNNNNNNGC 6 cut(s) 955, 1706, 1739, 1866, 2255, 2469
BstNI CCWGG 4 cut(s) 191, 261, 519, 1383
BstNSI RCATGY 3 cut(s) 626, 1888, 2306
BstSCI CCNGG 5 cut(s) 189, 259, 517, 1381, 1578
BstSFI CTRYAG 6 cut(s) 977, 1064, 1092, 1185, 2607, 2657
BstSLI GKGCMC 4 cut(s) 1045, 1744, 1771, 1823
BstUI CGCG 2 cut(s) 1586, 2463
BstV1I GCAGC 5 cut(s) 37, 285, 307, 317, 2593
BstV2I GAAGAC 1 cut(s) 2134
BstX2I RGATCY 5 cut(s) 199, 973, 1853, 2099, 2341
BstXI CCANNNNNNTGG 1 cut(s) 99
BstYI RGATCY 5 cut(s) 199, 973, 1853, 2099, 2341
BstZ17I GTATAC 1 cut(s) 2389
BsuRI GGCC 7 cut(s) 74, 189, 779, 798, 1374, 1583, 2582
BtsCI GGATG 8 cut(s) 236, 503, 512, 2239, 2494, 2676, 2739, 2775
BveI ACCTGC 2 cut(s) 149, 433
Cac8I GCNNGC 3 cut(s) 328, 1533, 2181
CaiI CAGNNNCTG 1 cut(s) 2043
CfoI GCGC 2 cut(s) 605, 1588
Cfr10I RCCGGY 2 cut(s) 105, 413
CseI GACGC 1 cut(s) 1722
Csp6I GTAC 7 cut(s) 902, 942, 1105, 1291, 1638, 2468, 2500
CspAI ACCGGT 2 cut(s) 105, 413
CspCI CAANNNNNGTGG 4 cut(s) 631, 666, 898, 933
CviAII CATG 8 cut(s) 184, 623, 1885, 2174, 2303, 2356, 2473, 2584
CviQI GTAC 7 cut(s) 902, 942, 1105, 1291, 1638, 2468, 2500
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 2580
Eam1104I CTCTTC 4 cut(s) 702, 2372, 2490, 2701
EarI CTCTTC 4 cut(s) 702, 2372, 2490, 2701
Ecl136II GAGCTC 1 cut(s) 1922
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 1924
Eco31I GGTCTC 1 cut(s) 1163
Eco32I GATATC 1 cut(s) 2336
Eco47III AGCGCT 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 1922
Eco88I CYCGRG 1 cut(s) 1229
EcoICRI GAGCTC 1 cut(s) 1922
EcoO109I RGGNCCY 2 cut(s) 797, 2546
EcoRII CCWGG 4 cut(s) 189, 259, 517, 1381
EcoRV GATATC 1 cut(s) 2336
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 1006
EcoT38I GRGCYC 1 cut(s) 1924
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 8 cut(s) 187, 626, 1888, 2177, 2306, 2359, 2476, 2587
FalI AAGNNNNNCTT 4 cut(s) 22, 54, 25, 57
FaqI GGGAC 5 cut(s) 116, 667, 716, 738, 1530
FatI CATG 8 cut(s) 183, 622, 1884, 2173, 2302, 2355, 2472, 2583
FblI GTMKAC 1 cut(s) 2388
Fnu4HI GCNGC 8 cut(s) 51, 296, 299, 331, 1529, 1584, 1861, 2607
FokI GGATG 7 cut(s) 223, 490, 499, 2246, 2501, 2683, 2726
FriOI GRGCYC 1 cut(s) 1924
Fsp4HI GCNGC 8 cut(s) 51, 296, 299, 331, 1529, 1584, 1861, 2607
FspBI CTAG 5 cut(s) 323, 533, 1346, 1901, 2162
GlaI GCGC 2 cut(s) 604, 1587
GluI GCNGC 8 cut(s) 51, 296, 299, 331, 1529, 1584, 1861, 2607
GsaI CCCAGC 4 cut(s) 57, 99, 103, 1749
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 7 cut(s) 74, 189, 779, 798, 1374, 1583, 2582
HapII CCGG 4 cut(s) 106, 414, 1579, 1857
HgaI GACGC 1 cut(s) 1722
HhaI GCGC 2 cut(s) 605, 1588
Hin1II CATG 8 cut(s) 187, 626, 1888, 2177, 2306, 2359, 2476, 2587
Hin6I GCGC 2 cut(s) 603, 1586
HinP1I GCGC 2 cut(s) 603, 1586
HincII GTYRAC 2 cut(s) 483, 2370
HindII GTYRAC 2 cut(s) 483, 2370
HindIII AAGCTT 2 cut(s) 956, 2082
HinfI GANTC 9 cut(s) 135, 152, 685, 1174, 1486, 1665, 2130, 2359, 2600
HpaI GTTAAC 1 cut(s) 2370
HpaII CCGG 4 cut(s) 106, 414, 1579, 1857
HphI GGTGA 4 cut(s) 130, 221, 898, 1040
Hpy166II GTNNAC 9 cut(s) 440, 483, 1043, 1769, 1821, 1876, 2049, 2370, 2389
Hpy8I GTNNAC 9 cut(s) 440, 483, 1043, 1769, 1821, 1876, 2049, 2370, 2389
Hpy99I CGWCG 1 cut(s) 2755
HpyAV CCTTC 7 cut(s) 27, 76, 125, 394, 1274, 2437, 2760
HpyCH4IV ACGT 1 cut(s) 1696
HpyF10VI GCNNNNNNNGC 6 cut(s) 955, 1706, 1739, 1866, 2255, 2469
HpySE526I ACGT 1 cut(s) 1696
Hsp92II CATG 8 cut(s) 187, 626, 1888, 2177, 2306, 2359, 2476, 2587
HspAI GCGC 2 cut(s) 603, 1586
KspAI GTTAAC 1 cut(s) 2370
LguI GCTCTTC 1 cut(s) 702
LmnI GCTCC 4 cut(s) 1570, 1784, 1807, 2736
Lsp1109I GCAGC 5 cut(s) 37, 285, 307, 317, 2593
LweI GCATC 3 cut(s) 1315, 2030, 2145
MaeI CTAG 5 cut(s) 323, 533, 1346, 1901, 2162
MaeII ACGT 1 cut(s) 1696
MaeIII GTNAC 4 cut(s) 160, 209, 848, 1087
MboII GAAGA 9 cut(s) 689, 1402, 1421, 2139, 2389, 2507, 2609, 2688, 2712
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 5 cut(s) 199, 973, 1853, 2099, 2341
MhlI GDGCHC 7 cut(s) 1045, 1303, 1744, 1771, 1823, 1924, 2733
MlsI TGGCCA 1 cut(s) 2582
MluNI TGGCCA 1 cut(s) 2582
MlyI GAGTC 4 cut(s) 679, 1674, 2124, 2353
MmeI TCCRAC 2 cut(s) 1648, 2642
Mox20I TGGCCA 1 cut(s) 2582
Mph1103I ATGCAT 1 cut(s) 1006
MroXI GAANNNNTTC 4 cut(s) 37, 959, 1406, 2652
MscI TGGCCA 1 cut(s) 2582
MslI CAYNNNNRTG 1 cut(s) 2477
Msp20I TGGCCA 1 cut(s) 2582
MspA1I CMGCKG 4 cut(s) 99, 298, 1749, 2606
MspCI CTTAAG 1 cut(s) 1811
MspI CCGG 4 cut(s) 106, 414, 1579, 1857
MspR9I CCNGG 5 cut(s) 191, 261, 519, 1383, 1580
MunI CAATTG 1 cut(s) 1629
Mva1269I GAATGC 1 cut(s) 2106
MvaI CCWGG 4 cut(s) 191, 261, 519, 1383
MvnI CGCG 2 cut(s) 1586, 2463
MwoI GCNNNNNNNGC 6 cut(s) 955, 1706, 1739, 1866, 2255, 2469
NciI CCSGG 1 cut(s) 1580
NlaIII CATG 8 cut(s) 187, 626, 1888, 2177, 2306, 2359, 2476, 2587
NlaIV GGNNCC 9 cut(s) 104, 726, 1133, 1465, 1546, 1743, 1855, 2343, 2548
NmuCI GTSAC 3 cut(s) 160, 209, 1087
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 3 cut(s) 626, 1888, 2306
PaqCI CACCTGC 1 cut(s) 149
PciI ACATGT 2 cut(s) 622, 2302
PciSI GCTCTTC 1 cut(s) 702
PctI GAATGC 1 cut(s) 2106
PdmI GAANNNNTTC 4 cut(s) 37, 959, 1406, 2652
PfeI GAWTC 5 cut(s) 135, 152, 1174, 1486, 2600
PinAI ACCGGT 2 cut(s) 105, 413
PkrI GCNGC 8 cut(s) 52, 297, 300, 332, 1530, 1585, 1862, 2608
PleI GAGTC 4 cut(s) 679, 1673, 2124, 2353
PpsI GAGTC 4 cut(s) 679, 1673, 2124, 2353
PpuMI RGGWCCY 1 cut(s) 2546
PscI ACATGT 2 cut(s) 622, 2302
PshBI ATTAAT 1 cut(s) 1118
Psp124BI GAGCTC 1 cut(s) 1924
Psp5II RGGWCCY 1 cut(s) 2546
Psp6I CCWGG 4 cut(s) 189, 259, 517, 1381
PspFI CCCAGC 4 cut(s) 53, 95, 99, 1745
PspGI CCWGG 4 cut(s) 189, 259, 517, 1381
PspN4I GGNNCC 9 cut(s) 104, 726, 1133, 1465, 1546, 1743, 1855, 2343, 2548
PspPPI RGGWCCY 1 cut(s) 2546
PstI CTGCAG 2 cut(s) 1068, 2611
PstNI CAGNNNCTG 1 cut(s) 2043
PsuI RGATCY 5 cut(s) 199, 973, 1853, 2099, 2341
PvuII CAGCTG 3 cut(s) 99, 298, 2606
RsaI GTAC 7 cut(s) 903, 943, 1106, 1292, 1639, 2469, 2501
RsaNI GTAC 7 cut(s) 902, 942, 1105, 1291, 1638, 2468, 2500
RseI CAYNNNNRTG 1 cut(s) 2477
SacI GAGCTC 1 cut(s) 1924
SapI GCTCTTC 1 cut(s) 702
SatI GCNGC 8 cut(s) 51, 296, 299, 331, 1529, 1584, 1861, 2607
ScaI AGTACT 1 cut(s) 2501
SchI GAGTC 4 cut(s) 679, 1674, 2124, 2353
ScrFI CCNGG 5 cut(s) 191, 261, 519, 1383, 1580
SduI GDGCHC 7 cut(s) 1045, 1303, 1744, 1771, 1823, 1924, 2733
SfaNI GCATC 3 cut(s) 1315, 2030, 2145
SfcI CTRYAG 6 cut(s) 977, 1064, 1092, 1185, 2607, 2657
SmiMI CAYNNNNRTG 1 cut(s) 2477
SmlI CTYRAG 4 cut(s) 689, 1397, 1811, 1949
SmoI CTYRAG 4 cut(s) 689, 1397, 1811, 1949
SpeI ACTAGT 2 cut(s) 532, 1900
SsiI CCGC 8 cut(s) 507, 1178, 1529, 1584, 1749, 1860, 2134, 2461
SspI AATATT 2 cut(s) 313, 2224
SspMI CTAG 5 cut(s) 323, 533, 1346, 1901, 2162
SstI GAGCTC 1 cut(s) 1924
StyD4I CCNGG 5 cut(s) 189, 259, 517, 1381, 1578
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 1699
TaqI TCGA 3 cut(s) 1364, 2115, 2527
TaqII GACCGA 1 cut(s) 1994
TatI WGTACW 3 cut(s) 1104, 1290, 2499
TauI GCSGC 3 cut(s) 1531, 1586, 1863
TfiI GAWTC 5 cut(s) 135, 152, 1174, 1486, 2600
TseFI GTSAC 3 cut(s) 160, 209, 1087
TseI GCWGC 5 cut(s) 50, 295, 298, 330, 2606
Tsp45I GTSAC 3 cut(s) 160, 209, 1087
TspGWI ACGGA 1 cut(s) 1263
Vha464I CTTAAG 1 cut(s) 1811
VneI GTGCAC 3 cut(s) 1041, 1767, 1819
VspI ATTAAT 1 cut(s) 1118
XapI RAATTY 3 cut(s) 335, 772, 1111
XceI RCATGY 3 cut(s) 626, 1888, 2306
XcmI CCANNNNNNNNNTGG 2 cut(s) 1734, 2227
XmiI GTMKAC 1 cut(s) 2388
XmnI GAANNNNTTC 4 cut(s) 37, 959, 1406, 2652
XspI CTAG 5 cut(s) 323, 533, 1346, 1901, 2162
ZrmI AGTACT 1 cut(s) 2501
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.