Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004327

Physical Location & Seq

Genomic Coordinates Reverse (-)

29211652 .. 29212249

Gene Structure 598 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1206100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 291 bp

ATGCTGGCTTCTTGTTCAAACTGTACGGACTACCAGTCAAGAAAGTTAGAAATTCGATATGGGCAGAAAAAGAGCAAAGAGCAGACAAAGCAATATGTTCACTTATTGAACTCGACTCTTACAGCGACTGAGAGAACTGTATGCTGCATTCTCGAGAACTACCAGACTGAAGAGGGTGTAAACGTACCAAAAGTTCTTCAACCTTTCATGGGTGGAAAGGAGTTCCTACCATTCAAGGAGAAACCGCCCCCTGAAGTCGAAGGGAAGAAATCGAAGGCCTACAATTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004828 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006434 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Seryl_tRNA_N tRNA-synt_2b

Protein Analysis

97

Amino Acids

10.96

Weight (kDa)

8.9

Isoelectric Point (pI)

51.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	2 - 54	1.5e-07	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27470
fragaria_vesca	FvH4_3g37770 FvH4_6g05710
malus_domestica	MD04G1206100.v1.1 MD04G1206400.v1.1 MD12G1220200.v1.1
prunus_persica	Prupe.6G327000_v2.0.a1
pyrus_communis	pycom04g18250 pycom12g20570
rosa_chinensis	RchiOBHm_Chr2g0115881 RchiOBHm_Chr3g0454601
rosa_laevigata	RLG00000018221 RLG00000019452 RLG00000025430 RLG00000031813
rosa_multiflora	Rmu_co8405619.1_g000001 Rmu_sc0001792.1_g000036 Rmu_ssc0000197.1_g000016
rosa_roxburghii	Rroxscaffold_1G00065260 Rroxscaffold_6G00424940
rosa_rugosa	Rorug02G0200700 Rorug03G0000500 Rorug04G0455300 Rorug05G0211400
rosa_samantha	Rh2BG268600 Rh2CG264100 Rh3AG060400 Rh3BG062500 Rh3CG061300 Rh3DG062100
rosa_wichuraiana	Rw2G020190 Rw3G004700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	245
AcsI	RAATTY	1 cut(s)	51
AcuI	CTGAAG	2 cut(s)	189, 273
AfaI	GTAC	2 cut(s)	25, 186
AfiI	CCNNNNNNNGG	1 cut(s)	209
AgsI	TTSAA	4 cut(s)	18, 109, 200, 235
AhdI	GACNNNNNGTC	1 cut(s)	34
AlwNI	CAGNNNCTG	1 cut(s)	128
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	1 cut(s)	276
ApeKI	GCWGC	1 cut(s)	144
ApoI	RAATTY	1 cut(s)	51
AvaI	CYCGRG	1 cut(s)	152
BbvI	GCAGC	1 cut(s)	131
BisI	GCNGC	1 cut(s)	145
BlsI	GCNGC	1 cut(s)	146
BmeRI	GACNNNNNGTC	1 cut(s)	34
BmeT110I	CYCGRG	1 cut(s)	152
Bsc4I	CCNNNNNNNGG	1 cut(s)	209
Bse1I	ACTGG	1 cut(s)	34
BseLI	CCNNNNNNNGG	1 cut(s)	209
BseMII	CTCAG	1 cut(s)	120
BseNI	ACTGG	1 cut(s)	34
BseXI	GCAGC	1 cut(s)	131
BshFI	GGCC	1 cut(s)	278
BsiHKCI	CYCGRG	1 cut(s)	152
BslI	CCNNNNNNNGG	1 cut(s)	209
BsmI	GAATGC	1 cut(s)	147
BsnI	GGCC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	152
BspACI	CCGC	1 cut(s)	245
BspANI	GGCC	1 cut(s)	278
BspCNI	CTCAG	1 cut(s)	121
BsrI	ACTGG	1 cut(s)	34
Bst4CI	ACNGT	2 cut(s)	23, 139
Bst6I	CTCTTC	1 cut(s)	165
BstC8I	GCNNGC	1 cut(s)	6
BstDEI	CTNAG	1 cut(s)	129
BstMWI	GCNNNNNNNGC	1 cut(s)	88
BstV1I	GCAGC	1 cut(s)	131
BsuRI	GGCC	1 cut(s)	278
Cac8I	GCNNGC	1 cut(s)	6
CaiI	CAGNNNCTG	1 cut(s)	128
Csp6I	GTAC	2 cut(s)	24, 185
CviAII	CATG	1 cut(s)	208
CviJI	RGCY	2 cut(s)	8, 278
CviKI_1	RGCY	2 cut(s)	8, 278
CviQI	GTAC	2 cut(s)	24, 185
DdeI	CTNAG	1 cut(s)	129
DriI	GACNNNNNGTC	1 cut(s)	34
Eam1104I	CTCTTC	1 cut(s)	165
Eam1105I	GACNNNNNGTC	1 cut(s)	34
EarI	CTCTTC	1 cut(s)	165
Eco147I	AGGCCT	1 cut(s)	278
Eco57I	CTGAAG	2 cut(s)	189, 273
Eco88I	CYCGRG	1 cut(s)	152
FaeI	CATG	1 cut(s)	211
FaiI	YATR	4 cut(s)	60, 96, 142, 209
FatI	CATG	1 cut(s)	207
Fnu4HI	GCNGC	1 cut(s)	145
Fsp4HI	GCNGC	1 cut(s)	145
GluI	GCNGC	1 cut(s)	145
HaeIII	GGCC	1 cut(s)	278
Hin1II	CATG	1 cut(s)	211
HinfI	GANTC	1 cut(s)	115
Hpy166II	GTNNAC	2 cut(s)	100, 181
Hpy188III	TCNNGA	3 cut(s)	39, 152, 154
Hpy8I	GTNNAC	2 cut(s)	100, 181
HpyAV	CCTTC	2 cut(s)	254, 268
HpyCH4III	ACNGT	2 cut(s)	23, 139
HpyCH4IV	ACGT	1 cut(s)	183
HpyCH4V	TGCA	1 cut(s)	147
HpyF10VI	GCNNNNNNNGC	1 cut(s)	88
HpyF3I	CTNAG	1 cut(s)	129
HpySE526I	ACGT	1 cut(s)	183
Hsp92II	CATG	1 cut(s)	211
LpnPI	CCDG	3 cut(s)	47, 176, 264
Lsp1109I	GCAGC	1 cut(s)	131
MaeII	ACGT	1 cut(s)	183
MboII	GAAGA	3 cut(s)	182, 188, 277
MluCI	AATT	2 cut(s)	51, 283
MlyI	GAGTC	1 cut(s)	109
MnlI	CCTC	1 cut(s)	166
Mva1269I	GAATGC	1 cut(s)	147
MwoI	GCNNNNNNNGC	1 cut(s)	88
NlaIII	CATG	1 cut(s)	211
PaeR7I	CTCGAG	1 cut(s)	152
PceI	AGGCCT	1 cut(s)	278
PctI	GAATGC	1 cut(s)	147
PkrI	GCNGC	1 cut(s)	146
PleI	GAGTC	1 cut(s)	109
PpsI	GAGTC	1 cut(s)	109
PsrI	GAACNNNNNNTAC	2 cut(s)	177, 209
PstNI	CAGNNNCTG	1 cut(s)	128
RsaI	GTAC	2 cut(s)	25, 186
RsaNI	GTAC	2 cut(s)	24, 185
SatI	GCNGC	1 cut(s)	145
SchI	GAGTC	1 cut(s)	109
SetI	ASST	2 cut(s)	186, 205
Sfr274I	CTCGAG	1 cut(s)	152
SlaI	CTCGAG	1 cut(s)	152
SmlI	CTYRAG	1 cut(s)	152
SmoI	CTYRAG	1 cut(s)	152
Sse9I	AATT	2 cut(s)	51, 283
SseBI	AGGCCT	1 cut(s)	278
SsiI	CCGC	1 cut(s)	245
StuI	AGGCCT	1 cut(s)	278
TaaI	ACNGT	2 cut(s)	23, 139
TaiI	ACGT	1 cut(s)	186
TaqI	TCGA	5 cut(s)	55, 113, 153, 258, 272
TasI	AATT	2 cut(s)	51, 283
TseI	GCWGC	1 cut(s)	144
TspDTI	ATGAA	1 cut(s)	196
TspGWI	ACGGA	1 cut(s)	41
XapI	RAATTY	1 cut(s)	51
XhoI	CTCGAG	1 cut(s)	152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.