Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004327

Physical Location & Seq

Genomic Coordinates Reverse (-)

29709105 .. 29713430

Gene Structure 4326 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1220200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGCTGGACATCAATCTGTTCAGAGAGGAGAAGGGCCACAACCCCGAAATAATCCGGGAATCTCAACGACGACGTTTCAAAGGCGTCGAGATCGTCGACGAGGTCATCCAGCTCGACAAAGAATGGCGACAGCGTCAATTCGAGCTCGAGAGCCTTCGGAAAGAGTTCAACAAGATCAACAAGCGAGTTGCTCAGCTGAGGATTGCAGGAGAGGATGCAACTGAGGTGATTAAAGACACAGAGGAAAATAAAAGGTTGGCAGCAGAGAAAGAAGTTGAAGTGCGAGAAGCTTTAACACAGTTGAATTCAAAACTGGACATCATTGGAAACCTTGTGCATGATTCAGTTCCCTTTAGCAATGATGAGGAAAATAATGCTGTGATTAGATCATGGGGTGACAAACGAGTGGAGCCAAAACTCAAAAACCATGTTGAACTTGTTGAGCTTCTTGGGATCGCAGACTTGAAGAAAGGCGCTGAAGTGGCAGGAGGTAGAGGTTTTTACCTGAAAGGTGTTGGTGTACGCCTTAATCAAGCTCTGATAAACTTTGGTCTTGATTTTCTTGAGAAAAGGGGATATACAGAACTGCATACCCCATTTTTCATGAGAAAAGATATCATGGGAAAATGTGCTCAATTAGCACAATTTGATGAGGAACTTTACAAGGTTACCGGTGAAGGTGATGACAAATATCTGATTGCTACATCTGAACAGCCACTTTGTGCATATCATATAGATGATTGGATCCATCCATCCCAGCTACCCTTAAGATATGCGGGATATTCATCTTGTTTTCGTAAAGAAGCTGGTTCACACGGTCGAGATACCTTGGGAATTTTTAGGGTTCATCAGTTTGAGAAAGTTGAACAGTTCTGTATTACAACCCCAAATGGCAATGATTCTTGGGAAATGCACGAGGAAATGATAAAAAACTCTGAGGAATTTTATCAAGCGTTAGGGCTCCCCTATCATATTGTGTCCATCGTTTCTGGTGCTTTGAATGATGCCGCAGCAAAGAAGTATGATCTGGAAGGATGGTTTCCTGCATCTCAGACATATAGAGAGCTGGTTTCTTGTTCAAACTGTACGGACTACCAGTCAAGAAAGTTAGAAATTCGATATGGGCAGAAAAAGAGCAACGAGCAGACAAAGCAATATGTTCACTTATTGAACTCAACTCTTACTGCGACAGAGAGAACCATGTGCTGCATTCTCGAGAACTACCAGACCGAAGAGGGTGTAAATGTACCAAAAGTTCTTCAACCTTTCATGGGTGGAAAGGAGTTCCTACCGTTCAAGGAGAAACCGGTCCCTGAAGTCAAAGGGAAGAAATCGAAGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004828 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006434 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Seryl_tRNA_N tRNA-synt_2b

Protein Analysis

448

Amino Acids

51.39

Weight (kDa)

5.99

Isoelectric Point (pI)

42.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Seryl_tRNA_N	PF02403	1 - 112	2.8e-24	Seryl-tRNA synthetase N-terminal domain
tRNA-synt_2b	PF00587	221 - 408	3e-44	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27470
fragaria_vesca	FvH4_3g37770 FvH4_6g05710
malus_domestica	MD04G1206100.v1.1 MD04G1206400.v1.1 MD12G1220200.v1.1
prunus_persica	Prupe.6G327000_v2.0.a1
pyrus_communis	pycom04g18250 pycom12g20570
rosa_chinensis	RchiOBHm_Chr2g0115881 RchiOBHm_Chr3g0454601
rosa_laevigata	RLG00000018221 RLG00000019452 RLG00000025430 RLG00000031813
rosa_multiflora	Rmu_co8405619.1_g000001 Rmu_sc0001792.1_g000036 Rmu_ssc0000197.1_g000016
rosa_roxburghii	Rroxscaffold_1G00065260 Rroxscaffold_6G00424940
rosa_rugosa	Rorug02G0200700 Rorug03G0000500 Rorug04G0455300 Rorug05G0211400
rosa_samantha	Rh2BG268600 Rh2CG264100 Rh3AG060400 Rh3BG062500 Rh3CG061300 Rh3DG062100
rosa_wichuraiana	Rw2G020190 Rw3G004700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	96
AciI	CCGC	2 cut(s)	776, 1008
AclWI	GGATC	3 cut(s)	461, 739, 752
AcsI	RAATTY	4 cut(s)	304, 834, 941, 1113
AcuI	CTGAAG	2 cut(s)	498, 1335
AcyI	GRCGYC	1 cut(s)	84
AdeI	CACNNNGTG	1 cut(s)	722
AfaI	GTAC	3 cut(s)	522, 1087, 1248
AfiI	CCNNNNNNNGG	1 cut(s)	1271
AflII	CTTAAG	1 cut(s)	766
AgeI	ACCGGT	2 cut(s)	671, 1306
AhdI	GACNNNNNGTC	1 cut(s)	1096
AluBI	AGCT	9 cut(s)	112, 145, 196, 290, 445, 536, 760, 806, 1066
AluI	AGCT	9 cut(s)	112, 145, 196, 290, 445, 536, 760, 806, 1066
Alw21I	GWGCWC	2 cut(s)	147, 634
AlwI	GGATC	3 cut(s)	461, 739, 752
Ama87I	CYCGRG	2 cut(s)	146, 1214
AoxI	GGCC	2 cut(s)	34, 1338
ApeKI	GCWGC	3 cut(s)	260, 1010, 1206
ApoI	RAATTY	4 cut(s)	304, 834, 941, 1113
AsiGI	ACCGGT	2 cut(s)	671, 1306
Asp700I	GAANNNNTTC	1 cut(s)	164
AspLEI	GCGC	1 cut(s)	476
AspS9I	GGNCC	2 cut(s)	34, 1309
AsuC2I	CCSGG	1 cut(s)	56
AsuHPI	GGTGA	4 cut(s)	238, 407, 686, 692
AvaI	CYCGRG	2 cut(s)	146, 1214
AvaII	GGWCC	1 cut(s)	1309
BamHI	GGATCC	1 cut(s)	744
BanII	GRGCYC	2 cut(s)	147, 963
BauI	CACGAG	1 cut(s)	914
Bbv12I	GWGCWC	2 cut(s)	147, 634
BbvCI	CCTCAGC	1 cut(s)	197
BbvI	GCAGC	3 cut(s)	272, 1022, 1193
BccI	CCATC	4 cut(s)	756, 760, 989, 1029
BcnI	CCSGG	1 cut(s)	56
BfoI	RGCGCY	1 cut(s)	477
BfrI	CTTAAG	1 cut(s)	766
BisI	GCNGC	4 cut(s)	261, 1008, 1011, 1207
BlpI	GCTNAGC	1 cut(s)	192
BlsI	GCNGC	4 cut(s)	262, 1009, 1012, 1208
Bme1390I	CCNGG	1 cut(s)	56
Bme18I	GGWCC	1 cut(s)	1309
BmeRI	GACNNNNNGTC	1 cut(s)	1096
BmeT110I	CYCGRG	2 cut(s)	146, 1214
BmgT120I	GGNCC	2 cut(s)	34, 1309
BmiI	GGNNCC	4 cut(s)	411, 746, 962, 1311
BmrFI	CCNGG	1 cut(s)	56
BmsI	GCATC	3 cut(s)	205, 994, 1055
Bpu10I	CCTNAGC	1 cut(s)	197
Bpu1102I	GCTNAGC	1 cut(s)	192
BpuEI	CTTGAG	1 cut(s)	584
BpuMI	CCSGG	1 cut(s)	56
BsaBI	GATNNNNATC	1 cut(s)	784
BsaHI	GRCGYC	1 cut(s)	84
BsaJI	CCNNGG	1 cut(s)	828
BsaWI	WCCGGW	2 cut(s)	671, 1306
Bsc4I	CCNNNNNNNGG	1 cut(s)	1271
Bse118I	RCCGGY	2 cut(s)	671, 1306
Bse1I	ACTGG	2 cut(s)	318, 1096
Bse3DI	GCAATG	2 cut(s)	364, 901
Bse8I	GATNNNNATC	1 cut(s)	784
BseDI	CCNNGG	1 cut(s)	828
BseGI	GGATG	5 cut(s)	105, 220, 748, 752, 1040
BseJI	GATNNNNATC	1 cut(s)	784
BseLI	CCNNNNNNNGG	1 cut(s)	1271
BseMI	GCAATG	2 cut(s)	364, 901
BseMII	CTCAG	5 cut(s)	188, 206, 213, 927, 1064
BseNI	ACTGG	2 cut(s)	318, 1096
BseRI	GAGGAG	1 cut(s)	41
BseXI	GCAGC	3 cut(s)	272, 1022, 1193
BseYI	CCCAGC	1 cut(s)	756
Bsh1285I	CGRYCG	1 cut(s)	820
BshFI	GGCC	2 cut(s)	36, 1340
BshTI	ACCGGT	2 cut(s)	671, 1306
BsiEI	CGRYCG	1 cut(s)	820
BsiHKAI	GWGCWC	2 cut(s)	147, 634
BsiHKCI	CYCGRG	2 cut(s)	146, 1214
BsiSI	CCGG	3 cut(s)	55, 672, 1307
BslFI	GGGAC	1 cut(s)	1295
BslI	CCNNNNNNNGG	1 cut(s)	1271
BsmFI	GGGAC	1 cut(s)	1295
BsmI	GAATGC	1 cut(s)	1209
BsnI	GGCC	2 cut(s)	36, 1340
BsoBI	CYCGRG	2 cut(s)	146, 1214
Bsp1286I	GDGCHC	3 cut(s)	147, 634, 963
Bsp143I	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
Bsp1720I	GCTNAGC	1 cut(s)	192
BspACI	CCGC	2 cut(s)	776, 1008
BspANI	GGCC	2 cut(s)	36, 1340
BspCNI	CTCAG	5 cut(s)	189, 205, 214, 928, 1063
BspHI	TCATGA	1 cut(s)	603
BspLI	GGNNCC	4 cut(s)	411, 746, 962, 1311
BspPI	GGATC	3 cut(s)	461, 739, 752
BspTI	CTTAAG	1 cut(s)	766
BsrDI	GCAATG	2 cut(s)	364, 901
BsrFI	RCCGGY	2 cut(s)	671, 1306
BsrI	ACTGG	2 cut(s)	318, 1096
BssAI	RCCGGY	2 cut(s)	671, 1306
BssECI	CCNNGG	1 cut(s)	828
BssMI	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
BssNI	GRCGYC	1 cut(s)	84
BssSI	CACGAG	1 cut(s)	914
BssT1I	CCWWGG	1 cut(s)	828
Bst2BI	CACGAG	1 cut(s)	914
Bst4CI	ACNGT	5 cut(s)	300, 818, 870, 1085, 1293
Bst6I	CTCTTC	1 cut(s)	1227
BstACI	GRCGYC	1 cut(s)	84
BstAFI	CTTAAG	1 cut(s)	766
BstDEI	CTNAG	5 cut(s)	192, 197, 222, 936, 1050
BstEII	GGTNACC	1 cut(s)	667
BstF5I	GGATG	5 cut(s)	105, 220, 748, 752, 1040
BstH2I	RGCGCY	1 cut(s)	477
BstHHI	GCGC	1 cut(s)	476
BstKTI	GATC	6 cut(s)	93, 177, 389, 456, 747, 1027
BstMBI	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
BstMCI	CGRYCG	1 cut(s)	820
BstMWI	GCNNNNNNNGC	3 cut(s)	482, 638, 1150
BstPI	GGTNACC	1 cut(s)	667
BstSCI	CCNGG	1 cut(s)	54
BstV1I	GCAGC	3 cut(s)	272, 1022, 1193
BstX2I	RGATCY	1 cut(s)	744
BstYI	RGATCY	1 cut(s)	744
BsuRI	GGCC	2 cut(s)	36, 1340
BtsCI	GGATG	5 cut(s)	105, 220, 748, 752, 1040
CciI	TCATGA	1 cut(s)	603
CfoI	GCGC	1 cut(s)	476
Cfr10I	RCCGGY	2 cut(s)	671, 1306
Cfr13I	GGNCC	2 cut(s)	34, 1309
CseI	GACGC	2 cut(s)	73, 122
Csp6I	GTAC	3 cut(s)	521, 1086, 1247
CspAI	ACCGGT	2 cut(s)	671, 1306
CviAII	CATG	7 cut(s)	338, 390, 428, 604, 619, 1201, 1270
CviQI	GTAC	3 cut(s)	521, 1086, 1247
DdeI	CTNAG	5 cut(s)	192, 197, 222, 936, 1050
DpnI	GATC	6 cut(s)	92, 176, 388, 455, 746, 1026
DpnII	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
DraIII	CACNNNGTG	1 cut(s)	722
DriI	GACNNNNNGTC	1 cut(s)	1096
Eam1104I	CTCTTC	1 cut(s)	1227
Eam1105I	GACNNNNNGTC	1 cut(s)	1096
EarI	CTCTTC	1 cut(s)	1227
Ecl136II	GAGCTC	1 cut(s)	145
Eco130I	CCWWGG	1 cut(s)	828
Eco147I	AGGCCT	1 cut(s)	1340
Eco24I	GRGCYC	2 cut(s)	147, 963
Eco32I	GATATC	1 cut(s)	616
Eco47I	GGWCC	1 cut(s)	1309
Eco53kI	GAGCTC	1 cut(s)	145
Eco57I	CTGAAG	2 cut(s)	498, 1335
Eco88I	CYCGRG	2 cut(s)	146, 1214
Eco91I	GGTNACC	1 cut(s)	667
EcoICRI	GAGCTC	1 cut(s)	145
EcoO65I	GGTNACC	1 cut(s)	667
EcoRI	GAATTC	1 cut(s)	304
EcoRV	GATATC	1 cut(s)	616
EcoT14I	CCWWGG	1 cut(s)	828
EcoT38I	GRGCYC	2 cut(s)	147, 963
ErhI	CCWWGG	1 cut(s)	828
FaeI	CATG	7 cut(s)	341, 393, 431, 607, 622, 1204, 1273
FaqI	GGGAC	1 cut(s)	1295
FatI	CATG	7 cut(s)	337, 389, 427, 603, 618, 1200, 1269
FauI	CCCGC	1 cut(s)	769
FblI	GTMKAC	1 cut(s)	96
Fnu4HI	GCNGC	4 cut(s)	261, 1008, 1011, 1207
FokI	GGATG	5 cut(s)	92, 227, 735, 739, 1047
FriOI	GRGCYC	2 cut(s)	147, 963
Fsp4HI	GCNGC	4 cut(s)	261, 1008, 1011, 1207
GlaI	GCGC	1 cut(s)	475
GluI	GCNGC	4 cut(s)	261, 1008, 1011, 1207
GsaI	CCCAGC	1 cut(s)	760
HaeII	RGCGCY	1 cut(s)	477
HaeIII	GGCC	2 cut(s)	36, 1340
HapII	CCGG	3 cut(s)	55, 672, 1307
HgaI	GACGC	2 cut(s)	73, 122
HhaI	GCGC	1 cut(s)	476
Hin1I	GRCGYC	1 cut(s)	84
Hin1II	CATG	7 cut(s)	341, 393, 431, 607, 622, 1204, 1273
Hin6I	GCGC	1 cut(s)	474
HinP1I	GCGC	1 cut(s)	474
HincII	GTYRAC	1 cut(s)	97
HindII	GTYRAC	1 cut(s)	97
HindIII	AAGCTT	1 cut(s)	288
HinfI	GANTC	3 cut(s)	59, 341, 899
HpaII	CCGG	3 cut(s)	55, 672, 1307
HphI	GGTGA	4 cut(s)	238, 407, 686, 692
Hpy166II	GTNNAC	4 cut(s)	97, 521, 812, 1162
Hpy188I	TCNGA	7 cut(s)	23, 159, 540, 696, 709, 937, 1053
Hpy8I	GTNNAC	4 cut(s)	97, 521, 812, 1162
Hpy99I	CGWCG	5 cut(s)	72, 75, 89, 98, 101
HpyAV	CCTTC	5 cut(s)	25, 164, 671, 1025, 1330
HpyCH4III	ACNGT	5 cut(s)	300, 818, 870, 1085, 1293
HpyCH4IV	ACGT	1 cut(s)	73
HpyCH4V	TGCA	8 cut(s)	206, 218, 337, 589, 725, 913, 1046, 1209
HpyF10VI	GCNNNNNNNGC	3 cut(s)	482, 638, 1150
HpyF3I	CTNAG	5 cut(s)	192, 197, 222, 936, 1050
HpySE526I	ACGT	1 cut(s)	73
Hsp92I	GRCGYC	1 cut(s)	84
Hsp92II	CATG	7 cut(s)	341, 393, 431, 607, 622, 1204, 1273
HspAI	GCGC	1 cut(s)	474
Kzo9I	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
LmnI	GCTCC	2 cut(s)	409, 966
Lsp1109I	GCAGC	3 cut(s)	272, 1022, 1193
LweI	GCATC	3 cut(s)	205, 994, 1055
MaeII	ACGT	1 cut(s)	73
MaeIII	GTNAC	2 cut(s)	395, 667
MalI	GATC	6 cut(s)	92, 176, 388, 455, 746, 1026
MboI	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
MboII	GAAGA	4 cut(s)	478, 1244, 1250, 1339
MflI	RGATCY	1 cut(s)	744
MhlI	GDGCHC	3 cut(s)	147, 634, 963
MluCI	AATT	7 cut(s)	137, 304, 635, 644, 834, 941, 1113
MroXI	GAANNNNTTC	1 cut(s)	164
MseI	TTAA	4 cut(s)	231, 293, 528, 767
MslI	CAYNNNNRTG	1 cut(s)	735
MspA1I	CMGCKG	1 cut(s)	196
MspCI	CTTAAG	1 cut(s)	766
MspI	CCGG	3 cut(s)	55, 672, 1307
MspR9I	CCNGG	1 cut(s)	56
Mva1269I	GAATGC	1 cut(s)	1209
MwoI	GCNNNNNNNGC	3 cut(s)	482, 638, 1150
NciI	CCSGG	1 cut(s)	56
NdeII	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
NlaIII	CATG	7 cut(s)	341, 393, 431, 607, 622, 1204, 1273
NlaIV	GGNNCC	4 cut(s)	411, 746, 962, 1311
NmuCI	GTSAC	1 cut(s)	395
PaeR7I	CTCGAG	2 cut(s)	146, 1214
PagI	TCATGA	1 cut(s)	603
PceI	AGGCCT	1 cut(s)	1340
PcsI	WCGNNNNNNNCGW	1 cut(s)	93
PctI	GAATGC	1 cut(s)	1209
PdmI	GAANNNNTTC	1 cut(s)	164
PfeI	GAWTC	3 cut(s)	59, 341, 899
PflFI	GACNNNGTC	2 cut(s)	101, 132
PfoI	TCCNGGA	1 cut(s)	54
PinAI	ACCGGT	2 cut(s)	671, 1306
PkrI	GCNGC	4 cut(s)	262, 1009, 1012, 1208
Psp124BI	GAGCTC	1 cut(s)	147
PspEI	GGTNACC	1 cut(s)	667
PspFI	CCCAGC	1 cut(s)	756
PspN4I	GGNNCC	4 cut(s)	411, 746, 962, 1311
PspPI	GGNCC	2 cut(s)	34, 1309
PsrI	GAACNNNNNNTAC	2 cut(s)	1239, 1271
PsuI	RGATCY	1 cut(s)	744
PsyI	GACNNNGTC	2 cut(s)	101, 132
PvuII	CAGCTG	1 cut(s)	196
RsaI	GTAC	3 cut(s)	522, 1087, 1248
RsaNI	GTAC	3 cut(s)	521, 1086, 1247
RseI	CAYNNNNRTG	1 cut(s)	735
SacI	GAGCTC	1 cut(s)	147
SalI	GTCGAC	1 cut(s)	95
SaqAI	TTAA	4 cut(s)	231, 293, 528, 767
SatI	GCNGC	4 cut(s)	261, 1008, 1011, 1207
Sau3AI	GATC	6 cut(s)	90, 174, 386, 453, 744, 1024
Sau96I	GGNCC	2 cut(s)	34, 1309
ScrFI	CCNGG	1 cut(s)	56
SduI	GDGCHC	3 cut(s)	147, 634, 963
SfaNI	GCATC	3 cut(s)	205, 994, 1055
Sfr274I	CTCGAG	2 cut(s)	146, 1214
SgrDI	CGTCGACG	1 cut(s)	95
SinI	GGWCC	1 cut(s)	1309
SlaI	CTCGAG	2 cut(s)	146, 1214
SmiMI	CAYNNNNRTG	1 cut(s)	735
SmlI	CTYRAG	4 cut(s)	146, 563, 766, 1214
SmoI	CTYRAG	4 cut(s)	146, 563, 766, 1214
Sse9I	AATT	7 cut(s)	137, 304, 635, 644, 834, 941, 1113
SseBI	AGGCCT	1 cut(s)	1340
SsiI	CCGC	2 cut(s)	776, 1008
SstI	GAGCTC	1 cut(s)	147
StuI	AGGCCT	1 cut(s)	1340
StyD4I	CCNGG	1 cut(s)	54
StyI	CCWWGG	1 cut(s)	828
TaaI	ACNGT	5 cut(s)	300, 818, 870, 1085, 1293
TaiI	ACGT	1 cut(s)	76
TaqI	TCGA	9 cut(s)	87, 96, 114, 141, 147, 820, 1117, 1215, 1334
TaqII	GACCGA	1 cut(s)	1244
TasI	AATT	7 cut(s)	137, 304, 635, 644, 834, 941, 1113
TauI	GCSGC	1 cut(s)	1010
TfiI	GAWTC	3 cut(s)	59, 341, 899
Tru1I	TTAA	4 cut(s)	231, 293, 528, 767
Tru9I	TTAA	4 cut(s)	231, 293, 528, 767
TseFI	GTSAC	1 cut(s)	395
TseI	GCWGC	3 cut(s)	260, 1010, 1206
Tsp45I	GTSAC	1 cut(s)	395
TspDTI	ATGAA	4 cut(s)	592, 774, 836, 1258
TspGWI	ACGGA	1 cut(s)	1103
Tth111I	GACNNNGTC	2 cut(s)	101, 132
Vha464I	CTTAAG	1 cut(s)	766
VpaK11BI	GGWCC	1 cut(s)	1309
XapI	RAATTY	4 cut(s)	304, 834, 941, 1113
XhoI	CTCGAG	2 cut(s)	146, 1214
XmiI	GTMKAC	1 cut(s)	96
XmnI	GAANNNNTTC	1 cut(s)	164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.