Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0004327

Physical Location & Seq

Genomic Coordinates Reverse (-)

86995772 .. 86998575

Gene Structure 2804 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00065260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGCTGGACTTAAATCTGTTTAGAGAAAGGAAGGACCACGACCCCGACGTCATCCTCAAGTCGCAGGCCGTCGTTTCAAGAGCGCTGATCTTGTTGACAAGGACAATTCGAGCTCGTGAATCTCAGGAAAGAGTTCAACCAGACCAACAAGGAAATTGGCGCAAACACAGAAAGGGTTTAGGAGCTGAAGTTCAAGAAGCTTTAGCACAACTGAATTCAAGCTTGGAAAAAATTGGGAACCTTGTCCATGATTCAGTTCCCGTCAGCGAAGATGAGGCAAACAATGAAGTGGTTAGATCATGGGGAGAGAACCGGATGGAGCCAGCACTGAAAAGTCATGTCGAGCTTTGCAAGCTTCTTGGGATTGCTGACTTTGAGAAAGGTGCTAAAGTTTCTGGATCCAGAGGTTATTACATTGAAGGCGTTGGCATAGATCTTCATGAAGCTCTCAAAAACTTTGCTCTAGATTTTCTTGAGGACAAGGGATTCAAAAAAATGATTCCTCCATTTTCTATGAGACAAGATGTCATGGCCAAATGTGCTCAACTACAGGAATTCGATAATGAACTCTATAAGGTAACTGGTGAAGGAGACGATAAGTATTTGATTGCTACCGCAGAACAGCCTCTATGTGCTCGTCACAAGGATGGATGGTTCCATCCCTCCCAGCTACCCATAAAATATGCTGGATTTTCATGTTGCTTCCGGACAGAAGCTGGCTCGCATGGAAGAGATACATTGGGGCTTTTTAGGACTCATGAGTTTGCCAAAGTAGAACAATTCTGTATTACCAGCCCAAATGAAAATGCCTCTTGGGATATGCATGATGAGATGATCAGAAACTCAGAGGAGTTCTATAAAGCTCTCAAGATTCCATATCGTGTAGTCAATATTGTTTCTGGTGCTTTGAATGGTGCAGCAGCAAAGAAGTATGACTTAGAAGGTTGGTTTCCTGCATCTCAGACGTATAGGGAACTAGTCTCATGTTCAAACTGTACAGACTATCAGTCCAGAAAATTAGAAATTCGATATGGACAGACAGGCAATGAGAAGACAGAGCAGTACGTTCACATGTTGAACTCCACTCTTACCGCAATTGGGAGAACGATGTGCTGCATTCTCGAAAACTACCAAACGAAAGATGGTGTCGAGCTACCAGAACGGGAATATTGGGCCCAGCATGAATTGCCAACCCTGAAGGATACCATTCCTCCTGCTTTCCGCTATTTCATCAATAAGTTCTATAGTGTTTTTGTAACATCAATGCCTGATGATCTTCACAGTAATGTCGTTTTTGGTGCATTATCAGACCCATTAAAAACTATGAATGATCTGCTACACACTCGCAAGGGCAAGAAGCAAGCATTGAGGATACTGAGGAAAAAAGCCTTGAATCGTGCAAAATTAACAGAAGATCAAGTATTGCAGAAAATCGAGGAAAAAACTGCAGCAAGGAAGAACAAAAAGTACGAAGATTCAGATAAAATTAGAAAAGATTTAGCTGCTGTGGTAATCACTCTTACAGACAACCCAGATGGGACAACATGTCTCCCTCTTGCAATTCAAGAGCAGCAGGTGGCATCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004828 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006434 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Seryl_tRNA_N tRNA-synt_2b

Protein Analysis

529

Amino Acids

60.19

Weight (kDa)

6.72

Isoelectric Point (pI)

36.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	191 - 377	6.1e-40	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27470
fragaria_vesca	FvH4_3g37770 FvH4_6g05710
malus_domestica	MD04G1206100.v1.1 MD04G1206400.v1.1 MD12G1220200.v1.1
prunus_persica	Prupe.6G327000_v2.0.a1
pyrus_communis	pycom04g18250 pycom12g20570
rosa_chinensis	RchiOBHm_Chr2g0115881 RchiOBHm_Chr3g0454601
rosa_laevigata	RLG00000018221 RLG00000019452 RLG00000025430 RLG00000031813
rosa_multiflora	Rmu_co8405619.1_g000001 Rmu_sc0001792.1_g000036 Rmu_ssc0000197.1_g000016
rosa_roxburghii	Rroxscaffold_1G00065260 Rroxscaffold_6G00424940
rosa_rugosa	Rorug02G0200700 Rorug03G0000500 Rorug04G0455300 Rorug05G0211400
rosa_samantha	Rh2BG268600 Rh2CG264100 Rh3AG060400 Rh3BG062500 Rh3CG061300 Rh3DG062100
rosa_wichuraiana	Rw2G020190 Rw3G004700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1564
AasI	GACNNNNNNGTC	1 cut(s)	47
AatII	GACGTC	1 cut(s)	51
Acc36I	ACCTGC	1 cut(s)	1564
AccIII	TCCGGA	1 cut(s)	705
AciI	CCGC	3 cut(s)	615, 1092, 1222
AclWI	GGATC	2 cut(s)	393, 406
AcoI	YGGCCR	1 cut(s)	531
AcsI	RAATTY	3 cut(s)	214, 554, 1023
AcuI	CTGAAG	2 cut(s)	207, 1217
AcyI	GRCGYC	1 cut(s)	48
AfaI	GTAC	3 cut(s)	997, 1064, 1469
AfeI	AGCGCT	1 cut(s)	84
AfiI	CCNNNNNNNGG	1 cut(s)	1098
AflIII	ACRYGT	2 cut(s)	1071, 1544
AhdI	GACNNNNNGTC	3 cut(s)	524, 1006, 1545
AhlI	ACTAGT	1 cut(s)	976
AjuI	GAANNNNNNNTTGG	2 cut(s)	206, 238
AloI	GAACNNNNNNTCC	4 cut(s)	174, 206, 638, 670
Alw21I	GWGCWC	3 cut(s)	115, 544, 637
Alw26I	GTCTC	4 cut(s)	511, 585, 985, 1553
AlwI	GGATC	2 cut(s)	393, 406
AlwNI	CAGNNNCTG	1 cut(s)	716
Aor13HI	TCCGGA	1 cut(s)	705
Aor51HI	AGCGCT	1 cut(s)	84
AoxI	GGCC	3 cut(s)	66, 531, 1173
ApaI	GGGCCC	1 cut(s)	1177
ApeKI	GCWGC	6 cut(s)	917, 920, 1113, 1448, 1502, 1570
ApoI	RAATTY	3 cut(s)	214, 554, 1023
Asp700I	GAANNNNTTC	1 cut(s)	132
AspLEI	GCGC	2 cut(s)	85, 162
AspS9I	GGNCC	3 cut(s)	34, 1173, 1174
AsuHPI	GGTGA	1 cut(s)	596
AvaII	GGWCC	1 cut(s)	34
BaeGI	GKGCMC	1 cut(s)	1177
BalI	TGGCCA	1 cut(s)	533
BamHI	GGATCC	1 cut(s)	398
BanII	GRGCYC	2 cut(s)	115, 1177
BauI	CACGAG	1 cut(s)	114
BbsI	GAAGAC	1 cut(s)	1058
Bbv12I	GWGCWC	3 cut(s)	115, 544, 637
BbvI	GCAGC	6 cut(s)	929, 932, 1100, 1460, 1489, 1582
BccI	CCATC	6 cut(s)	310, 641, 645, 666, 1136, 1529
BceAI	ACGGC	1 cut(s)	53
BciVI	GTATCC	2 cut(s)	1195, 1365
BclI	TGATCA	1 cut(s)	834
BcoDI	GTCTC	4 cut(s)	511, 585, 985, 1553
BcuI	ACTAGT	1 cut(s)	976
BfaI	CTAG	2 cut(s)	464, 977
BfmI	CTRYAG	3 cut(s)	548, 1243, 1446
BfoI	RGCGCY	1 cut(s)	86
BfuAI	ACCTGC	1 cut(s)	1564
BfuI	GTATCC	2 cut(s)	1195, 1365
BglII	AGATCT	1 cut(s)	433
BisI	GCNGC	6 cut(s)	918, 921, 1114, 1449, 1503, 1571
BlsI	GCNGC	6 cut(s)	919, 922, 1115, 1450, 1504, 1572
Bme18I	GGWCC	1 cut(s)	34
BmeRI	GACNNNNNGTC	3 cut(s)	524, 1006, 1545
BmgT120I	GGNCC	3 cut(s)	34, 1173, 1174
BmiI	GGNNCC	5 cut(s)	239, 321, 400, 656, 1175
BmsI	GCATC	1 cut(s)	965
BpiI	GAAGAC	1 cut(s)	1058
BpuEI	CTTGAG	3 cut(s)	41, 494, 851
BsaHI	GRCGYC	1 cut(s)	48
BsaWI	WCCGGW	2 cut(s)	312, 705
BsaXI	ACNNNNNCTCC	4 cut(s)	174, 204, 1195, 1225
Bsc4I	CCNNNNNNNGG	1 cut(s)	1098
Bse1I	ACTGG	1 cut(s)	586
Bse3DI	GCAATG	1 cut(s)	1051
BseAI	TCCGGA	1 cut(s)	705
BseGI	GGATG	5 cut(s)	51, 321, 652, 656, 658
BseLI	CCNNNNNNNGG	1 cut(s)	1098
BseMI	GCAATG	1 cut(s)	1051
BseMII	CTCAG	4 cut(s)	137, 858, 974, 1367
BseNI	ACTGG	1 cut(s)	586
BseRI	GAGGAG	1 cut(s)	863
BseSI	GKGCMC	1 cut(s)	1177
BseXI	GCAGC	6 cut(s)	929, 932, 1100, 1460, 1489, 1582
BseYI	CCCAGC	2 cut(s)	666, 1176
BsgI	GTGCAG	1 cut(s)	936
BshFI	GGCC	3 cut(s)	68, 533, 1175
BsiHKAI	GWGCWC	3 cut(s)	115, 544, 637
BsiSI	CCGG	2 cut(s)	313, 706
BslFI	GGGAC	1 cut(s)	1552
BslI	CCNNNNNNNGG	1 cut(s)	1098
BsmAI	GTCTC	4 cut(s)	511, 585, 985, 1553
BsmBI	CGTCTC	1 cut(s)	585
BsmFI	GGGAC	1 cut(s)	1552
BsmI	GAATGC	1 cut(s)	1116
BsnI	GGCC	3 cut(s)	68, 533, 1175
Bsp120I	GGGCCC	1 cut(s)	1173
Bsp1286I	GDGCHC	4 cut(s)	115, 544, 637, 1177
Bsp13I	TCCGGA	1 cut(s)	705
Bsp1407I	TGTACA	1 cut(s)	995
Bsp143I	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
BspACI	CCGC	3 cut(s)	615, 1092, 1222
BspANI	GGCC	3 cut(s)	68, 533, 1175
BspCNI	CTCAG	4 cut(s)	136, 857, 973, 1368
BspEI	TCCGGA	1 cut(s)	705
BspHI	TCATGA	2 cut(s)	439, 757
BspLI	GGNNCC	5 cut(s)	239, 321, 400, 656, 1175
BspMAI	CTGCAG	1 cut(s)	1450
BspMI	ACCTGC	1 cut(s)	1564
BspPI	GGATC	2 cut(s)	393, 406
BsrDI	GCAATG	1 cut(s)	1051
BsrGI	TGTACA	1 cut(s)	995
BsrI	ACTGG	1 cut(s)	586
BssMI	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
BssNI	GRCGYC	1 cut(s)	48
BssSI	CACGAG	1 cut(s)	114
Bst2BI	CACGAG	1 cut(s)	114
Bst4CI	ACNGT	2 cut(s)	995, 1283
Bst6I	CTCTTC	1 cut(s)	724
BstACI	GRCGYC	1 cut(s)	48
BstAPI	GCANNNNNTGC	1 cut(s)	1186
BstAUI	TGTACA	1 cut(s)	995
BstC8I	GCNNGC	6 cut(s)	66, 324, 353, 718, 722, 1362
BstDEI	CTNAG	5 cut(s)	123, 844, 937, 960, 1376
BstF5I	GGATG	5 cut(s)	51, 321, 652, 656, 658
BstH2I	RGCGCY	1 cut(s)	86
BstHHI	GCGC	2 cut(s)	85, 162
BstKTI	GATC	8 cut(s)	90, 299, 401, 436, 837, 1276, 1333, 1417
BstMAI	GTCTC	4 cut(s)	511, 585, 985, 1553
BstMBI	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
BstMWI	GCNNNNNNNGC	3 cut(s)	352, 539, 1186
BstNSI	RCATGY	2 cut(s)	1075, 1548
BstSFI	CTRYAG	3 cut(s)	548, 1243, 1446
BstSLI	GKGCMC	1 cut(s)	1177
BstV1I	GCAGC	6 cut(s)	929, 932, 1100, 1460, 1489, 1582
BstV2I	GAAGAC	1 cut(s)	1058
BstX2I	RGATCY	2 cut(s)	398, 433
BstYI	RGATCY	2 cut(s)	398, 433
BsuI	GTATCC	2 cut(s)	1195, 1365
BsuRI	GGCC	3 cut(s)	68, 533, 1175
BtsCI	GGATG	5 cut(s)	51, 321, 652, 656, 658
BtsIMutI	CAGTG	1 cut(s)	326
BveI	ACCTGC	1 cut(s)	1564
Cac8I	GCNNGC	6 cut(s)	66, 324, 353, 718, 722, 1362
CaiI	CAGNNNCTG	1 cut(s)	716
CciI	TCATGA	2 cut(s)	439, 757
CfoI	GCGC	2 cut(s)	85, 162
Cfr13I	GGNCC	3 cut(s)	34, 1173, 1174
Csp6I	GTAC	3 cut(s)	996, 1063, 1468
CviQI	GTAC	3 cut(s)	996, 1063, 1468
DdeI	CTNAG	5 cut(s)	123, 844, 937, 960, 1376
DpnI	GATC	8 cut(s)	89, 298, 400, 435, 836, 1275, 1332, 1416
DpnII	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
DrdI	GACNNNNNNGTC	1 cut(s)	47
DriI	GACNNNNNGTC	3 cut(s)	524, 1006, 1545
DseDI	GACNNNNNNGTC	1 cut(s)	47
EaeI	YGGCCR	1 cut(s)	531
Eam1104I	CTCTTC	1 cut(s)	724
Eam1105I	GACNNNNNGTC	3 cut(s)	524, 1006, 1545
EarI	CTCTTC	1 cut(s)	724
Ecl136II	GAGCTC	1 cut(s)	113
Eco24I	GRGCYC	2 cut(s)	115, 1177
Eco47I	GGWCC	1 cut(s)	34
Eco47III	AGCGCT	1 cut(s)	84
Eco53kI	GAGCTC	1 cut(s)	113
Eco57I	CTGAAG	2 cut(s)	207, 1217
EcoICRI	GAGCTC	1 cut(s)	113
EcoRI	GAATTC	2 cut(s)	214, 554
EcoT22I	ATGCAT	1 cut(s)	825
EcoT38I	GRGCYC	2 cut(s)	115, 1177
Esp3I	CGTCTC	1 cut(s)	585
FalI	AAGNNNNNCTT	2 cut(s)	920, 952
FaqI	GGGAC	1 cut(s)	1552
FbaI	TGATCA	1 cut(s)	834
Fnu4HI	GCNGC	6 cut(s)	918, 921, 1114, 1449, 1503, 1571
FokI	GGATG	5 cut(s)	38, 328, 645, 659, 663
FriOI	GRGCYC	2 cut(s)	115, 1177
Fsp4HI	GCNGC	6 cut(s)	918, 921, 1114, 1449, 1503, 1571
FspBI	CTAG	2 cut(s)	464, 977
GlaI	GCGC	2 cut(s)	84, 161
GluI	GCNGC	6 cut(s)	918, 921, 1114, 1449, 1503, 1571
GsaI	CCCAGC	2 cut(s)	670, 1180
HaeII	RGCGCY	1 cut(s)	86
HaeIII	GGCC	3 cut(s)	68, 533, 1175
HapII	CCGG	2 cut(s)	313, 706
HhaI	GCGC	2 cut(s)	85, 162
Hin1I	GRCGYC	1 cut(s)	48
Hin6I	GCGC	2 cut(s)	83, 160
HinP1I	GCGC	2 cut(s)	83, 160
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HindIII	AAGCTT	3 cut(s)	198, 220, 353
HinfI	GANTC	8 cut(s)	119, 251, 486, 499, 754, 871, 1393, 1475
HpaII	CCGG	2 cut(s)	313, 706
HphI	GGTGA	1 cut(s)	596
Hpy166II	GTNNAC	2 cut(s)	96, 1069
Hpy188I	TCNGA	5 cut(s)	839, 847, 963, 1309, 1480
Hpy8I	GTNNAC	2 cut(s)	96, 1069
Hpy99I	CGWCG	2 cut(s)	50, 74
HpyAV	CCTTC	5 cut(s)	25, 413, 581, 935, 1192
HpyCH4III	ACNGT	2 cut(s)	995, 1283
HpyCH4IV	ACGT	3 cut(s)	48, 965, 1065
HpyF10VI	GCNNNNNNNGC	3 cut(s)	352, 539, 1186
HpyF3I	CTNAG	5 cut(s)	123, 844, 937, 960, 1376
HpySE526I	ACGT	3 cut(s)	48, 965, 1065
Hsp92I	GRCGYC	1 cut(s)	48
HspAI	GCGC	2 cut(s)	83, 160
Kpn2I	TCCGGA	1 cut(s)	705
Ksp22I	TGATCA	1 cut(s)	834
Kzo9I	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
LmnI	GCTCC	2 cut(s)	182, 319
Lsp1109I	GCAGC	6 cut(s)	929, 932, 1100, 1460, 1489, 1582
LweI	GCATC	1 cut(s)	965
MaeI	CTAG	2 cut(s)	464, 977
MaeII	ACGT	3 cut(s)	48, 965, 1065
MaeIII	GTNAC	3 cut(s)	577, 638, 1255
MalI	GATC	8 cut(s)	89, 298, 400, 435, 836, 1275, 1332, 1416
MboI	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
MboII	GAAGA	8 cut(s)	281, 428, 741, 1063, 1268, 1424, 1468, 1484
MfeI	CAATTG	1 cut(s)	1095
MflI	RGATCY	2 cut(s)	398, 433
MhlI	GDGCHC	4 cut(s)	115, 544, 637, 1177
MlsI	TGGCCA	1 cut(s)	533
MluNI	TGGCCA	1 cut(s)	533
MlyI	GAGTC	1 cut(s)	748
Mox20I	TGGCCA	1 cut(s)	533
Mph1103I	ATGCAT	1 cut(s)	825
MroI	TCCGGA	1 cut(s)	705
MroXI	GAANNNNTTC	1 cut(s)	132
MscI	TGGCCA	1 cut(s)	533
MseI	TTAA	3 cut(s)	11, 1316, 1406
MslI	CAYNNNNRTG	2 cut(s)	645, 1284
Msp20I	TGGCCA	1 cut(s)	533
MspI	CCGG	2 cut(s)	313, 706
MunI	CAATTG	1 cut(s)	1095
Mva1269I	GAATGC	1 cut(s)	1116
MwoI	GCNNNNNNNGC	3 cut(s)	352, 539, 1186
NdeII	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
NlaIV	GGNNCC	5 cut(s)	239, 321, 400, 656, 1175
NmuCI	GTSAC	1 cut(s)	638
NsiI	ATGCAT	1 cut(s)	825
NspI	RCATGY	2 cut(s)	1075, 1548
PagI	TCATGA	2 cut(s)	439, 757
PaqCI	CACCTGC	1 cut(s)	1564
PciI	ACATGT	2 cut(s)	1071, 1544
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	1116
PdmI	GAANNNNTTC	1 cut(s)	132
PfeI	GAWTC	7 cut(s)	119, 251, 486, 499, 871, 1393, 1475
PkrI	GCNGC	6 cut(s)	919, 922, 1115, 1450, 1504, 1572
PleI	GAGTC	1 cut(s)	748
PpsI	GAGTC	1 cut(s)	748
PscI	ACATGT	2 cut(s)	1071, 1544
Psp124BI	GAGCTC	1 cut(s)	115
PspFI	CCCAGC	2 cut(s)	666, 1176
PspN4I	GGNNCC	5 cut(s)	239, 321, 400, 656, 1175
PspOMI	GGGCCC	1 cut(s)	1173
PspPI	GGNCC	3 cut(s)	34, 1173, 1174
PsrI	GAACNNNNNNTAC	2 cut(s)	1451, 1483
PstI	CTGCAG	1 cut(s)	1450
PstNI	CAGNNNCTG	1 cut(s)	716
PsuI	RGATCY	2 cut(s)	398, 433
RsaI	GTAC	3 cut(s)	997, 1064, 1469
RsaNI	GTAC	3 cut(s)	996, 1063, 1468
RseI	CAYNNNNRTG	2 cut(s)	645, 1284
SacI	GAGCTC	1 cut(s)	115
SaqAI	TTAA	3 cut(s)	11, 1316, 1406
SatI	GCNGC	6 cut(s)	918, 921, 1114, 1449, 1503, 1571
Sau3AI	GATC	8 cut(s)	87, 296, 398, 433, 834, 1273, 1330, 1414
Sau96I	GGNCC	3 cut(s)	34, 1173, 1174
SchI	GAGTC	1 cut(s)	748
SduI	GDGCHC	4 cut(s)	115, 544, 637, 1177
SfaNI	GCATC	1 cut(s)	965
SfcI	CTRYAG	3 cut(s)	548, 1243, 1446
SinI	GGWCC	1 cut(s)	34
SmiMI	CAYNNNNRTG	2 cut(s)	645, 1284
SmlI	CTYRAG	3 cut(s)	56, 473, 866
SmoI	CTYRAG	3 cut(s)	56, 473, 866
SpeI	ACTAGT	1 cut(s)	976
SsiI	CCGC	3 cut(s)	615, 1092, 1222
SspI	AATATT	2 cut(s)	892, 1169
SspMI	CTAG	2 cut(s)	464, 977
SstI	GAGCTC	1 cut(s)	115
TaaI	ACNGT	2 cut(s)	995, 1283
TaiI	ACGT	3 cut(s)	51, 968, 1068
TaqI	TCGA	7 cut(s)	109, 342, 558, 1027, 1122, 1149, 1434
TatI	WGTACW	1 cut(s)	995
TfiI	GAWTC	7 cut(s)	119, 251, 486, 499, 871, 1393, 1475
Tru1I	TTAA	3 cut(s)	11, 1316, 1406
Tru9I	TTAA	3 cut(s)	11, 1316, 1406
TscAI	CASTG	1 cut(s)	333
TseFI	GTSAC	1 cut(s)	638
TseI	GCWGC	6 cut(s)	917, 920, 1113, 1448, 1502, 1570
Tsp45I	GTSAC	1 cut(s)	638
TspDTI	ATGAA	9 cut(s)	300, 428, 456, 579, 684, 816, 1197, 1219, 1340
TspRI	CASTG	1 cut(s)	333
VpaK11BI	GGWCC	1 cut(s)	34
XapI	RAATTY	3 cut(s)	214, 554, 1023
XbaI	TCTAGA	1 cut(s)	463
XceI	RCATGY	2 cut(s)	1075, 1548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1139
XmnI	GAANNNNTTC	1 cut(s)	132
XspI	CTAG	2 cut(s)	464, 977
ZraI	GACGTC	1 cut(s)	49
Zsp2I	ATGCAT	1 cut(s)	825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.