Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

29756839 .. 29760352

Gene Structure 3514 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1214300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2913 bp

ATGGAGGTCGTAACAGTTGGGGTTTTTGTTTTGTTTCTTTTCTTTGTTTGGGACAGAAGCGCAACAATGGAGAAAAGGGAGTTGAAGCAGAGCCATCTTGCCCAGCAATGGAAGGAGGTTTTGGGTTCTGACGGTTCGACCCGGAATGTGCAGCTCCTGACCCGATTCCAAGAGCTCCAGGACTACTTTGACTCGGAACGGAACTCGCTTCTGACCCGAAACCGAGAGATTGAGGACCGGGAGAAGGAATGGGAAGCTAAAGTTTTAAACTTTAACTTGGAAGTGGAAGCGAAAGCCCGAAAATTGCAGGAAATCGAGAGTTTGGCAGAGGAGAAAGGAAAAGAGGTTGAGTTTAAAACTATAAAACTTGGTCTCATTCAGAAATCAATGGTGGAATGCTGTAATACACTCGAATCGAAAGAAGAAGTCATCGGGGCAATGGAGAAGAGGACCCGTAAACTCGCAGCGAAAAAGAGGGAACTTGAAGGATTGGTTGATAAGCTCGAATTTAGACGGAGACAAGTTGAGAAACTCGGTTTGGTTGATAGCACGGTCACTGAATGCCTGAATGAGGTTCAATTGGAAGGAAAGCAATTGCATTCGCGTGAAAGGTCGTTGCAAGGATGTTCCGGTGGAGTTGAAAACAAAGAGAGAATTGAAGAACTGGCCAAAGAGCTTGAGTTGGAACGAAAACAAATTGATTCAGTGATTCAAGAACGCCGACAACATTTTGATTCACAAGAAAAGATGTTAACAGAAGGCTCCCATGGACTTGAAATGAAAAAGAGGCAACTTGAAGAAAAGGCCAAAGAGCTTGACTTGAAACAAAAACAGTTTGATTCGGTGATTCAGGAGCGCCAAAAGCATTTGGATTCACAAGAAAGTTCGTTACAAGAGCGTTCCTGTGCACTTGAAGTGAAGGATAGGCTACTTGAGGATCAGATCAGAGAGTTTGAATCAAAGCAAAAACAGTTAGATTTGATGATTCAAGGACGACAACAACGTTTGGATTCCCAAGAAAAGATGTTACTAGTAAGTTCCCACGGACTTGAAATGAAAGAGAAGCAACTTGAAAAACAGGCCAAAGAGCTTGAGTTGAAACAAAAACAGTTTGATTTGATGGTTCATGAACACAAGAAGCATTTGGATTCACAAGAAAAGTTGTTACAAGACTGTTTCCATGAACTTCAAATGAAATGGTGGCAAGTTGAAGAACAGGCCAAAGAGCTCGAGTTGAAACGAAAACAATTTGATGCAATGGCTCAAGAAAGCCAGAAGCAATTCATTTCACAGGAAAAGTTGTTACATGAATTCTCCACTGGACTTGAAATGAAAGAGAGGAACCTTGAAGAACATGTCAAAGAGTTTGAGTCAAATCAAAATGTTTTTGTTTCGATGATTCAAGGAGACCAACGACATTTGGATTCCCAGAAAAAATGGTTGCAAGAACGTTCCCATGGACTCCAAATGAAAGAGAGGCAATTTGAAGAACAAGTCAAAGAGTTTGAATCAAAACAAAAACAATCTTATTCACTGATTCAAGAACGCCAACAACATTTGGATTCACAAGAAAATTTGTTACAGGAAAGTTCACATGGACTTAAAATGAGAGAGATGCAACTCCTAGAACAAGTCAAAGAGCTTGAATCGAAACAGAAAGAATTTGATTCGATTAGAAAATCCACGGAGGAACACATCCAAAACCTGAAATCCAAGGAGAAGACTAATGTCCATCCTGAAGTGGATAATGATCAGCAATTGCTATGTACCCCTGCTACCATTATCAATAGGGATGGAAGAGGCTTGCAGTTGCTCATGAATGAGCATTTGAAAAGAATTGATTTAGTTGGCACTGAAATCTCAACTGTTCTCCAGGCGTCATCTGACCCAGCAAAACTAGTTTTGAATGCAATGCAAGGGTTTTATCCTTCAAATTTGACTGGATACAACTCAGAGACGTCTGATTATGATTTGAGAGTTATTAGGAGGAGTTGTATTCTTTTGTTACAGGAGTTGAAGATATTCTCACCACAAATAAGTCCTCAGGTGAGAGAAGATGCATTGAATTTGGCAGCTGATTGGAAGGCTAAGATGACGATGGCAAGCGAGAATGATATGGAGGTTTTGGGCTTTTTGAAGCTTGTTACTGTGTATGACATCACAACTGCTTGTGATCCAAAGGAGGTTCATAGTCTTCTCGCTATCGTGGCTCAGCATGGACAAGCAACTGAGTTATGCCAGGCACTTAAGACCACAAACAAGGCACCCGCGAGCAACAGCATTTCTTTCCCCGTCAAAGTTGAGGAACCAGAATCTTCACCGGTTAGAAATGTACTTACTTCATCTTCTCCAAATCTTCAACCTAGTGCCACTACAGATGCAACAAATATGCAGGGCTCTCTAAACGAGCATTCAAGTGGTAACCCACCAATGCAGAATGAAATGTTTGTTGCCCTACAAATGTCATTGCACCCGGAAAAACTTGCTTTCAAGTTGATGAAAACTCATTTGGATCAATGCTGGGGGAACAGAGATGTTGGTCTTGGAGCAGTTGTCATGGAGAATACCATTTCCCTGTTGATTGATCTAGCAAAAGTCACACCTCTTGTTGGATCTGATGTGAAAAAAGATGCTAAAGAGCTAGCAGTCCAGTGGAAGGCAAAATTGAGAGCTGATTCTGAAAATTCCTTTGAGATTTTGGGGTTTTTGCAGTTTATATCTACATATGAATTGCTTTCTGCCTTTAACAAAGATGAGATTGTGAAGCTTTTTGGGATGATTTATCTGGATGAAATGGCTCTAAACTCACACCGGACACTTGGTTTTTCAGATAAGATCCCTGGTAAGTTTCTTGAAAATCGACATTCATACATATCGTTATGCTCGTGTTCCACTACCATTGCTCTTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

971

Amino Acids

112.15

Weight (kDa)

5.67

Isoelectric Point (pI)

54.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	598 - 755	3.6e-31	Frigida-like protein
Frigida	PF07899	801 - 948	5.4e-19	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1961
AccB1I	GGYRCC	1 cut(s)	2263
AccII	CGCG	2 cut(s)	604, 2270
AciI	CCGC	1 cut(s)	2268
AclI	AACGTT	2 cut(s)	1003, 1450
AclWI	GGATC	5 cut(s)	945, 2168, 2520, 2620, 2830
AcoI	YGGCCR	1 cut(s)	666
AcsI	RAATTY	7 cut(s)	506, 1310, 1573, 1661, 1933, 2065, 2683
AcuI	CTGAAG	1 cut(s)	1758
AcyI	GRCGYC	2 cut(s)	1877, 1958
AfaI	GTAC	2 cut(s)	1768, 2334
AfiI	CCNNNNNNNGG	5 cut(s)	108, 244, 571, 2609, 2656
AflII	CTTAAG	1 cut(s)	2246
AflIII	ACRYGT	1 cut(s)	1354
AgeI	ACCGGT	1 cut(s)	2320
AhlI	ACTAGT	2 cut(s)	1030, 1897
AjnI	CCWGG	4 cut(s)	177, 1872, 2238, 2839
AjuI	GAANNNNNNNTTGG	6 cut(s)	104, 136, 521, 553, 2492, 2524
Alw21I	GWGCWC	3 cut(s)	177, 910, 1230
Alw26I	GTCTC	4 cut(s)	377, 511, 1401, 1949
Alw44I	GTGCAC	1 cut(s)	906
AlwI	GGATC	5 cut(s)	945, 2168, 2520, 2620, 2830
AlwNI	CAGNNNCTG	1 cut(s)	157
Ama87I	CYCGRG	1 cut(s)	1229
AoxI	GGCC	4 cut(s)	666, 804, 1080, 1218
ApaLI	GTGCAC	1 cut(s)	906
ApeKI	GCWGC	3 cut(s)	151, 464, 2072
ApoI	RAATTY	7 cut(s)	506, 1310, 1573, 1661, 1933, 2065, 2683
AsiGI	ACCGGT	1 cut(s)	2320
Asp700I	GAANNNNTTC	2 cut(s)	1280, 2021
AspLEI	GCGC	2 cut(s)	62, 858
AspS9I	GGNCC	2 cut(s)	235, 450
AsuC2I	CCSGG	3 cut(s)	142, 239, 2474
AsuHPI	GGTGA	4 cut(s)	856, 2019, 2059, 2310
AsuNHI	GCTAGC	1 cut(s)	2641
AvaI	CYCGRG	1 cut(s)	1229
AvaII	GGWCC	2 cut(s)	235, 450
AxyI	CCTNAGG	1 cut(s)	2043
BaeGI	GKGCMC	1 cut(s)	910
BalI	TGGCCA	1 cut(s)	668
BanI	GGYRCC	1 cut(s)	2263
BanII	GRGCYC	3 cut(s)	177, 1230, 2399
BauI	CACGAG	1 cut(s)	2884
BbsI	GAAGAC	2 cut(s)	1727, 2186
Bbv12I	GWGCWC	3 cut(s)	177, 910, 1230
BbvI	GCAGC	3 cut(s)	163, 476, 2084
BccI	CCATC	5 cut(s)	102, 1114, 1740, 1787, 2092
BciT130I	CCWGG	4 cut(s)	179, 1874, 2240, 2841
BciVI	GTATCC	1 cut(s)	1937
BclI	TGATCA	1 cut(s)	1750
BcnI	CCSGG	3 cut(s)	142, 239, 2474
BcoDI	GTCTC	4 cut(s)	377, 511, 1401, 1949
BcuI	ACTAGT	2 cut(s)	1030, 1897
BfaI	CTAG	6 cut(s)	1031, 1625, 1898, 2364, 2588, 2642
BfmI	CTRYAG	1 cut(s)	2373
BfoI	RGCGCY	1 cut(s)	859
BfrI	CTTAAG	1 cut(s)	2246
BfuI	GTATCC	1 cut(s)	1937
BisI	GCNGC	3 cut(s)	152, 465, 2073
BlpI	GCTNAGC	1 cut(s)	2211
BlsI	GCNGC	3 cut(s)	153, 466, 2074
Bme1390I	CCNGG	7 cut(s)	142, 179, 239, 1874, 2240, 2474, 2841
Bme18I	GGWCC	2 cut(s)	235, 450
BmeT110I	CYCGRG	1 cut(s)	1229
BmgT120I	GGNCC	2 cut(s)	235, 450
BmiI	GGNNCC	5 cut(s)	452, 763, 1343, 2265, 2307
BmrFI	CCNGG	7 cut(s)	142, 179, 239, 1874, 2240, 2474, 2841
BmsI	GCATC	5 cut(s)	1243, 1605, 2047, 2368, 2620
BmtI	GCTAGC	1 cut(s)	2645
BoxI	GACNNNNGTC	1 cut(s)	1727
BpiI	GAAGAC	2 cut(s)	1727, 2186
BpmI	CTGGAG	2 cut(s)	161, 1856
Bpu1102I	GCTNAGC	1 cut(s)	2211
BpuEI	CTTGAG	4 cut(s)	698, 953, 1112, 1248
BpuMI	CCSGG	3 cut(s)	142, 239, 2474
BsaBI	GATNNNNATC	1 cut(s)	1749
BsaHI	GRCGYC	2 cut(s)	1877, 1958
BsaI	GGTCTC	2 cut(s)	377, 1401
BsaJI	CCNNGG	6 cut(s)	766, 1042, 1456, 1683, 1713, 2839
BsaWI	WCCGGW	3 cut(s)	629, 2320, 2811
BsaXI	ACNNNNNCTCC	4 cut(s)	1978, 2008, 2538, 2568
Bsc4I	CCNNNNNNNGG	5 cut(s)	108, 244, 571, 2609, 2656
Bse118I	RCCGGY	1 cut(s)	2320
Bse1I	ACTGG	4 cut(s)	669, 1324, 1945, 2650
Bse21I	CCTNAGG	1 cut(s)	2043
Bse3DI	GCAATG	6 cut(s)	113, 444, 1263, 1917, 2465, 2898
Bse8I	GATNNNNATC	1 cut(s)	1749
BseBI	CCWGG	4 cut(s)	179, 1874, 2240, 2841
BseDI	CCNNGG	6 cut(s)	766, 1042, 1456, 1683, 1713, 2839
BseGI	GGATG	6 cut(s)	629, 1695, 1732, 1798, 2781, 2794
BseJI	GATNNNNATC	1 cut(s)	1749
BseLI	CCNNNNNNNGG	5 cut(s)	108, 244, 571, 2609, 2656
BseMI	GCAATG	6 cut(s)	113, 444, 1263, 1917, 2465, 2898
BseMII	CTCAG	4 cut(s)	1965, 2057, 2220, 2225
BseNI	ACTGG	4 cut(s)	669, 1324, 1945, 2650
BseRI	GAGGAG	2 cut(s)	344, 2002
BseSI	GKGCMC	1 cut(s)	910
BseXI	GCAGC	3 cut(s)	163, 476, 2084
BseYI	CCCAGC	3 cut(s)	102, 1888, 2520
BsgI	GTGCAG	1 cut(s)	170
Bsh1236I	CGCG	2 cut(s)	604, 2270
BshFI	GGCC	4 cut(s)	668, 806, 1082, 1220
BshNI	GGYRCC	1 cut(s)	2263
BshTI	ACCGGT	1 cut(s)	2320
BsiHKAI	GWGCWC	3 cut(s)	177, 910, 1230
BsiHKCI	CYCGRG	1 cut(s)	1229
BsiSI	CCGG	6 cut(s)	142, 238, 630, 2321, 2474, 2812
BslFI	GGGAC	1 cut(s)	65
BslI	CCNNNNNNNGG	5 cut(s)	108, 244, 571, 2609, 2656
BsmAI	GTCTC	4 cut(s)	377, 511, 1401, 1949
BsmBI	CGTCTC	1 cut(s)	1949
BsmFI	GGGAC	1 cut(s)	65
BsmI	GAATGC	5 cut(s)	401, 566, 598, 1912, 2410
BsnI	GGCC	4 cut(s)	668, 806, 1082, 1220
Bso31I	GGTCTC	2 cut(s)	377, 1401
BsoBI	CYCGRG	1 cut(s)	1229
Bsp1286I	GDGCHC	4 cut(s)	177, 910, 1230, 2399
Bsp143I	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
Bsp1720I	GCTNAGC	1 cut(s)	2211
Bsp19I	CCATGG	2 cut(s)	766, 1456
BspACI	CCGC	1 cut(s)	2268
BspANI	GGCC	4 cut(s)	668, 806, 1082, 1220
BspCNI	CTCAG	4 cut(s)	1964, 2056, 2221, 2224
BspFNI	CGCG	2 cut(s)	604, 2270
BspHI	TCATGA	2 cut(s)	1126, 1815
BspLI	GGNNCC	5 cut(s)	452, 763, 1343, 2265, 2307
BspOI	GCTAGC	1 cut(s)	2645
BspPI	GGATC	5 cut(s)	945, 2168, 2520, 2620, 2830
BspT107I	GGYRCC	1 cut(s)	2263
BspTI	CTTAAG	1 cut(s)	2246
BspTNI	GGTCTC	2 cut(s)	377, 1401
BsrDI	GCAATG	6 cut(s)	113, 444, 1263, 1917, 2465, 2898
BsrFI	RCCGGY	1 cut(s)	2320
BsrI	ACTGG	4 cut(s)	669, 1324, 1945, 2650
BssAI	RCCGGY	1 cut(s)	2320
BssECI	CCNNGG	6 cut(s)	766, 1042, 1456, 1683, 1713, 2839
BssMI	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
BssNI	GRCGYC	2 cut(s)	1877, 1958
BssSI	CACGAG	1 cut(s)	2884
BssT1I	CCWWGG	3 cut(s)	766, 1456, 1713
Bst2BI	CACGAG	1 cut(s)	2884
Bst2UI	CCWGG	4 cut(s)	179, 1874, 2240, 2841
Bst4CI	ACNGT	9 cut(s)	16, 134, 553, 834, 972, 1110, 1175, 1867, 2149
Bst6I	CTCTTC	2 cut(s)	440, 1792
BstACI	GRCGYC	2 cut(s)	1877, 1958
BstAFI	CTTAAG	1 cut(s)	2246
BstC8I	GCNNGC	4 cut(s)	1805, 2104, 2272, 2643
BstDEI	CTNAG	5 cut(s)	1951, 2043, 2088, 2211, 2229
BstDSI	CCRYGG	4 cut(s)	766, 1042, 1456, 1683
BstEII	GGTNACC	1 cut(s)	2420
BstENI	CCTNNNNNAGG	1 cut(s)	569
BstF5I	GGATG	6 cut(s)	629, 1695, 1732, 1798, 2781, 2794
BstFNI	CGCG	2 cut(s)	604, 2270
BstH2I	RGCGCY	1 cut(s)	859
BstHHI	GCGC	2 cut(s)	62, 858
BstKTI	GATC	8 cut(s)	940, 945, 1753, 2176, 2515, 2587, 2615, 2838
BstMAI	GTCTC	4 cut(s)	377, 511, 1401, 1949
BstMBI	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
BstMWI	GCNNNNNNNGC	2 cut(s)	862, 2207
BstNI	CCWGG	4 cut(s)	179, 1874, 2240, 2841
BstNSI	RCATGY	1 cut(s)	1358
BstPAI	GACNNNNGTC	1 cut(s)	1727
BstPI	GGTNACC	1 cut(s)	2420
BstSCI	CCNGG	7 cut(s)	140, 177, 237, 1872, 2238, 2472, 2839
BstSFI	CTRYAG	1 cut(s)	2373
BstSLI	GKGCMC	1 cut(s)	910
BstUI	CGCG	2 cut(s)	604, 2270
BstV1I	GCAGC	3 cut(s)	163, 476, 2084
BstV2I	GAAGAC	2 cut(s)	1727, 2186
BstX2I	RGATCY	2 cut(s)	2612, 2835
BstYI	RGATCY	2 cut(s)	2612, 2835
Bsu36I	CCTNAGG	1 cut(s)	2043
BsuI	GTATCC	1 cut(s)	1937
BsuRI	GGCC	4 cut(s)	668, 806, 1082, 1220
BtgI	CCRYGG	4 cut(s)	766, 1042, 1456, 1683
BtsCI	GGATG	6 cut(s)	629, 1695, 1732, 1798, 2781, 2794
BtsIMutI	CAGTG	6 cut(s)	555, 711, 1317, 1532, 1851, 2657
Cac8I	GCNNGC	4 cut(s)	1805, 2104, 2272, 2643
CaiI	CAGNNNCTG	1 cut(s)	157
CciI	TCATGA	2 cut(s)	1126, 1815
CfoI	GCGC	2 cut(s)	62, 858
Cfr10I	RCCGGY	1 cut(s)	2320
Cfr13I	GGNCC	2 cut(s)	235, 450
CseI	GACGC	1 cut(s)	1866
Csp6I	GTAC	2 cut(s)	1767, 2333
CspAI	ACCGGT	1 cut(s)	2320
CspCI	CAANNNNNGTGG	3 cut(s)	1306, 1341, 2881
CviQI	GTAC	2 cut(s)	1767, 2333
DdeI	CTNAG	5 cut(s)	1951, 2043, 2088, 2211, 2229
DpnI	GATC	8 cut(s)	939, 944, 1752, 2175, 2514, 2586, 2614, 2837
DpnII	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
DraI	TTTAAA	2 cut(s)	267, 355
EaeI	YGGCCR	1 cut(s)	666
Eam1104I	CTCTTC	2 cut(s)	440, 1792
EarI	CTCTTC	2 cut(s)	440, 1792
Ecl136II	GAGCTC	2 cut(s)	175, 1228
Eco130I	CCWWGG	3 cut(s)	766, 1456, 1713
Eco24I	GRGCYC	3 cut(s)	177, 1230, 2399
Eco31I	GGTCTC	2 cut(s)	377, 1401
Eco47I	GGWCC	2 cut(s)	235, 450
Eco53kI	GAGCTC	2 cut(s)	175, 1228
Eco57I	CTGAAG	1 cut(s)	1758
Eco81I	CCTNAGG	1 cut(s)	2043
Eco88I	CYCGRG	1 cut(s)	1229
Eco91I	GGTNACC	1 cut(s)	2420
EcoICRI	GAGCTC	2 cut(s)	175, 1228
EcoNI	CCTNNNNNAGG	1 cut(s)	569
EcoO109I	RGGNCCY	1 cut(s)	450
EcoO65I	GGTNACC	1 cut(s)	2420
EcoRI	GAATTC	1 cut(s)	1310
EcoRII	CCWGG	4 cut(s)	177, 1872, 2238, 2839
EcoT14I	CCWWGG	3 cut(s)	766, 1456, 1713
EcoT22I	ATGCAT	1 cut(s)	2062
EcoT38I	GRGCYC	3 cut(s)	177, 1230, 2399
ErhI	CCWWGG	3 cut(s)	766, 1456, 1713
Esp3I	CGTCTC	1 cut(s)	1949
FaqI	GGGAC	1 cut(s)	65
FauI	CCCGC	1 cut(s)	2275
FauNDI	CATATG	1 cut(s)	2725
FbaI	TGATCA	1 cut(s)	1750
Fnu4HI	GCNGC	3 cut(s)	152, 465, 2073
FokI	GGATG	6 cut(s)	636, 1682, 1719, 1805, 2788, 2801
FriOI	GRGCYC	3 cut(s)	177, 1230, 2399
Fsp4HI	GCNGC	3 cut(s)	152, 465, 2073
FspBI	CTAG	6 cut(s)	1031, 1625, 1898, 2364, 2588, 2642
GlaI	GCGC	2 cut(s)	61, 857
GluI	GCNGC	3 cut(s)	152, 465, 2073
GsaI	CCCAGC	3 cut(s)	106, 1892, 2524
GsuI	CTGGAG	2 cut(s)	161, 1856
HaeII	RGCGCY	1 cut(s)	859
HaeIII	GGCC	4 cut(s)	668, 806, 1082, 1220
HapII	CCGG	6 cut(s)	142, 238, 630, 2321, 2474, 2812
HgaI	GACGC	1 cut(s)	1866
HhaI	GCGC	2 cut(s)	62, 858
Hin1I	GRCGYC	2 cut(s)	1877, 1958
Hin6I	GCGC	2 cut(s)	60, 856
HinP1I	GCGC	2 cut(s)	60, 856
HincII	GTYRAC	1 cut(s)	753
HindII	GTYRAC	1 cut(s)	753
HindIII	AAGCTT	2 cut(s)	2138, 2765
HpaI	GTTAAC	1 cut(s)	753
HpaII	CCGG	6 cut(s)	142, 238, 630, 2321, 2474, 2812
HphI	GGTGA	4 cut(s)	856, 2019, 2059, 2310
Hpy166II	GTNNAC	4 cut(s)	458, 753, 908, 1592
Hpy8I	GTNNAC	4 cut(s)	458, 753, 908, 1592
HpyAV	CCTTC	9 cut(s)	106, 238, 479, 578, 752, 913, 1938, 2077, 2650
HpyCH4III	ACNGT	9 cut(s)	16, 134, 553, 834, 972, 1110, 1175, 1867, 2149
HpyCH4IV	ACGT	3 cut(s)	1003, 1450, 1958
HpyF10VI	GCNNNNNNNGC	2 cut(s)	862, 2207
HpyF3I	CTNAG	5 cut(s)	1951, 2043, 2088, 2211, 2229
HpySE526I	ACGT	3 cut(s)	1003, 1450, 1958
Hsp92I	GRCGYC	2 cut(s)	1877, 1958
HspAI	GCGC	2 cut(s)	60, 856
Ksp22I	TGATCA	1 cut(s)	1750
KspAI	GTTAAC	1 cut(s)	753
Kzo9I	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
LmnI	GCTCC	5 cut(s)	159, 180, 767, 853, 2546
Lsp1109I	GCAGC	3 cut(s)	163, 476, 2084
LweI	GCATC	5 cut(s)	1243, 1605, 2047, 2368, 2620
MaeI	CTAG	6 cut(s)	1031, 1625, 1898, 2364, 2588, 2642
MaeII	ACGT	3 cut(s)	1003, 1450, 1958
MalI	GATC	8 cut(s)	939, 944, 1752, 2175, 2514, 2586, 2614, 2837
MboI	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
MfeI	CAATTG	3 cut(s)	578, 593, 1757
MflI	RGATCY	2 cut(s)	2612, 2835
MhlI	GDGCHC	4 cut(s)	177, 910, 1230, 2399
MlsI	TGGCCA	1 cut(s)	668
MluNI	TGGCCA	1 cut(s)	668
MlyI	GAGTC	3 cut(s)	185, 1379, 1455
MmeI	TCCRAC	2 cut(s)	663, 2590
Mox20I	TGGCCA	1 cut(s)	668
Mph1103I	ATGCAT	1 cut(s)	2062
MroXI	GAANNNNTTC	2 cut(s)	1280, 2021
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	7 cut(s)	266, 273, 354, 752, 1602, 2247, 2745
MslI	CAYNNNNRTG	2 cut(s)	603, 2415
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	1 cut(s)	2075
MspCI	CTTAAG	1 cut(s)	2246
MspI	CCGG	6 cut(s)	142, 238, 630, 2321, 2474, 2812
MspR9I	CCNGG	7 cut(s)	142, 179, 239, 1874, 2240, 2474, 2841
MunI	CAATTG	3 cut(s)	578, 593, 1757
Mva1269I	GAATGC	5 cut(s)	401, 566, 598, 1912, 2410
MvaI	CCWGG	4 cut(s)	179, 1874, 2240, 2841
MvnI	CGCG	2 cut(s)	604, 2270
MwoI	GCNNNNNNNGC	2 cut(s)	862, 2207
NciI	CCSGG	3 cut(s)	142, 239, 2474
NcoI	CCATGG	2 cut(s)	766, 1456
NdeI	CATATG	1 cut(s)	2725
NdeII	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
NheI	GCTAGC	1 cut(s)	2641
NlaIV	GGNNCC	5 cut(s)	452, 763, 1343, 2265, 2307
NmuCI	GTSAC	2 cut(s)	553, 2596
NsiI	ATGCAT	1 cut(s)	2062
NspI	RCATGY	1 cut(s)	1358
PaeR7I	CTCGAG	1 cut(s)	1229
PagI	TCATGA	2 cut(s)	1126, 1815
PciI	ACATGT	1 cut(s)	1354
PcsI	WCGNNNNNNNCGW	2 cut(s)	1000, 2882
PctI	GAATGC	5 cut(s)	401, 566, 598, 1912, 2410
PdmI	GAANNNNTTC	2 cut(s)	1280, 2021
PfoI	TCCNGGA	1 cut(s)	177
PinAI	ACCGGT	1 cut(s)	2320
PkrI	GCNGC	3 cut(s)	153, 466, 2074
PleI	GAGTC	3 cut(s)	185, 1378, 1455
PpsI	GAGTC	3 cut(s)	185, 1378, 1455
PpuMI	RGGWCCY	1 cut(s)	450
PscI	ACATGT	1 cut(s)	1354
PshAI	GACNNNNGTC	1 cut(s)	1727
Psp124BI	GAGCTC	2 cut(s)	177, 1230
Psp1406I	AACGTT	2 cut(s)	1003, 1450
Psp5II	RGGWCCY	1 cut(s)	450
Psp6I	CCWGG	4 cut(s)	177, 1872, 2238, 2839
PspEI	GGTNACC	1 cut(s)	2420
PspFI	CCCAGC	3 cut(s)	102, 1888, 2520
PspGI	CCWGG	4 cut(s)	177, 1872, 2238, 2839
PspN4I	GGNNCC	5 cut(s)	452, 763, 1343, 2265, 2307
PspPI	GGNCC	2 cut(s)	235, 450
PspPPI	RGGWCCY	1 cut(s)	450
PspXI	VCTCGAGB	1 cut(s)	1229
PstNI	CAGNNNCTG	1 cut(s)	157
PsuI	RGATCY	2 cut(s)	2612, 2835
PvuII	CAGCTG	1 cut(s)	2075
RsaI	GTAC	2 cut(s)	1768, 2334
RsaNI	GTAC	2 cut(s)	1767, 2333
RseI	CAYNNNNRTG	2 cut(s)	603, 2415
SacI	GAGCTC	2 cut(s)	177, 1230
SaqAI	TTAA	7 cut(s)	266, 273, 354, 752, 1602, 2247, 2745
SatI	GCNGC	3 cut(s)	152, 465, 2073
Sau3AI	GATC	8 cut(s)	937, 942, 1750, 2173, 2512, 2584, 2612, 2835
Sau96I	GGNCC	2 cut(s)	235, 450
SchI	GAGTC	3 cut(s)	185, 1379, 1455
ScrFI	CCNGG	7 cut(s)	142, 179, 239, 1874, 2240, 2474, 2841
SduI	GDGCHC	4 cut(s)	177, 910, 1230, 2399
SfaNI	GCATC	5 cut(s)	1243, 1605, 2047, 2368, 2620
SfcI	CTRYAG	1 cut(s)	2373
Sfr274I	CTCGAG	1 cut(s)	1229
SinI	GGWCC	2 cut(s)	235, 450
SlaI	CTCGAG	1 cut(s)	1229
SmiMI	CAYNNNNRTG	2 cut(s)	603, 2415
SmlI	CTYRAG	6 cut(s)	677, 932, 1091, 1229, 1263, 2246
SmoI	CTYRAG	6 cut(s)	677, 932, 1091, 1229, 1263, 2246
SpeI	ACTAGT	2 cut(s)	1030, 1897
SsiI	CCGC	1 cut(s)	2268
SspMI	CTAG	6 cut(s)	1031, 1625, 1898, 2364, 2588, 2642
SstI	GAGCTC	2 cut(s)	177, 1230
StyD4I	CCNGG	7 cut(s)	140, 177, 237, 1872, 2238, 2472, 2839
StyI	CCWWGG	3 cut(s)	766, 1456, 1713
TaaI	ACNGT	9 cut(s)	16, 134, 553, 834, 972, 1110, 1175, 1867, 2149
TaiI	ACGT	3 cut(s)	1006, 1453, 1961
TatI	WGTACW	1 cut(s)	2332
Tru1I	TTAA	7 cut(s)	266, 273, 354, 752, 1602, 2247, 2745
Tru9I	TTAA	7 cut(s)	266, 273, 354, 752, 1602, 2247, 2745
TscAI	CASTG	6 cut(s)	562, 711, 1324, 1539, 1858, 2657
TseFI	GTSAC	2 cut(s)	553, 2596
TseI	GCWGC	3 cut(s)	151, 464, 2072
Tsp45I	GTSAC	2 cut(s)	553, 2596
TspGWI	ACGGA	4 cut(s)	214, 529, 1059, 1700
TspRI	CASTG	6 cut(s)	562, 711, 1324, 1539, 1858, 2657
Vha464I	CTTAAG	1 cut(s)	2246
VneI	GTGCAC	1 cut(s)	906
VpaK11BI	GGWCC	2 cut(s)	235, 450
XagI	CCTNNNNNAGG	1 cut(s)	569
XapI	RAATTY	7 cut(s)	506, 1310, 1573, 1661, 1933, 2065, 2683
XceI	RCATGY	1 cut(s)	1358
XhoI	CTCGAG	1 cut(s)	1229
XmnI	GAANNNNTTC	2 cut(s)	1280, 2021
XspI	CTAG	6 cut(s)	1031, 1625, 1898, 2364, 2588, 2642
ZraI	GACGTC	1 cut(s)	1959
Zsp2I	ATGCAT	1 cut(s)	2062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.