Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

46616774 .. 46630760

Gene Structure 13987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025551

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGGACAACATAACCTCTGATTTAGACCTCGCAGAGACGAAGCAGAGCAATCTGAGTAGAGCCTATGAGAGCCTACACACCCAAGCTTCTCCCTTTATGGTGCTAGGTCTTCAGTGGAAGGACCTCGAGGACCACCTGGACTCGACCCGGACCAAAATCCAGACCCGGTTCCACGAGCTCCAGGCCCGGGAAGCCCAAATCGCCGACCAGGTGAAAAAGCTTGAGTCTTTGAAGGAGGTTGAGTACGGTAAGAGTCACTTGCAGTCTTTAAAGTTGTTAATTGAAGAAACTAGTGAAGAGCTTGGTGTGAAAGAGAAGAGGTTTTCGGAAGTTGAGAGGTTGATTAGAGTGAAGGAGAGAATGCTTGAGGATAGGAATAAGATGTTGAGTTTGGTTGAGAGGAGGATAGAGGAGAAGGGTAGGGAGGTTGAGGGGAAGGAGAAGAAGGTGAGGGTGGTGCAAGGAGTGTTGGATAAGTACTGTGTTGATATTAGGAAGAGGAAGAAGGAGTGTGGTCTGAAAGGAAAGGAGGTTAAGAGGGTGGAGAGGTTGATTGATAAGTGTGATAAGGAGTTGAGTTTGAAGTGGGGGGAAATGGAAGGGATTCAGAAATCGATGGTGGCATATTCGGATAGGATTAAGTCGAAAGAGGGGGTTGTTAGGGAGATGGAGTTGAAGGTGAAGGAGTTTGGGATGCATAAGAAGGCAATGGAGGAGTGGTGTTGCAAGGTTGAAGTGAAGAAGAGGGAACTTGAAGTGTGGGTGGAGAAGGTTGAGCCGAGGGAGAGAGAGTTTGAGCCGAGAGTTGAGGAACTTGATTTGATTGGTAAGAGGGTTAATGAATGTCTCAATGAGGCTCAGTTGACTTTGGAAAGGGATTTTCGTTTGCTTGAGGAAATGAGACAGGAGAATGTGAAGCATTTTGAGTTAGTTGAGAAGTCAGTGCAAGAACGTTCTCTTGAACTTGAAATGAAAGAGAGGAGACTTGAAGAAAAGGCAAAAGAGCTTGACTTGAAACAAAAACTATTGGAATCTATTCCGAAAGCTACTGGAGAACAAATGAAATCGAAGGAGAAGGCTAGTATCCTTCATCCTCTAGTGAATATTCCACATATGTTCCCTGCAAACAATGTCGTCGTTCCTTCAACGGCAAGTAATGCTAGAGGTTTGCAGTTGATCATGAGGCAGCATTTGAAGAGAATTGATTTAATGAGTAGAGAAATATCAGCTCTTCGTCAAGCCTCAAGAGACCCAGCAGGATTGGTTTTGGATGCAATGCAGGGTTTTTACCCTACAAATTCAACTGTGGACAACAGGGAGCTTGATTCGGATCTAAGAGTGATTAGAAGGAGTTGTATTGTATTGCTACAGGAGTTAAAGAGATTCTCGCCACAAATTAATGCTCAGGTCAGAGAAAAGGCAATAAAGTTAGCCGCTGAGTGGAAGGCTAAGTTGGCGGTGACCACTGAGAATAGGTTGGAGGTGTTGGGCTTCTTGAGGCTTGTTACTACGTACGAATTGACCTCCATGTATGATCCAAAGGAGCTTCACAGTCTTCTCTCTATAGTTGTTCAGCCCGAACAAACAACTGAATTCCAGGCCCTTGGTGTCTCAGATAAGGCATTTGCGAGCAGCACAATTTCTTTCCCAGTTAGAATTGAGGAACCGGAATCTTCAGTGGCCAAATGTGTAGCCCCCTTTTCATCTCCAAATCTTCAACTAAGTGCAACCAGAGAACCAACAAATTTTCAGGGGTTCATAGTTGAGCGTTTGAGTGAGAATAATTCAGTACAGGACAAAATGTTGGCCACTCTTCAAGTATCACCAGACCCAGCACAAATTGTGTTGGAAATGATGCAAAGCTCTTTTGCTCAGTGTTGGAGAGAAGGAGGTTTTTTTTCAGAAGTAACTGTCATGAAGGGTTACATGTACCTACTGGAGACACTAATGAGAGAGTCAAAGCACATTGGGCCTCGTGTGAAAGAAGATGCAAGAAAGCTGGCAGTGCAGTGGAAAGCTAGAATGAGAGCCGATGCTGGCAACTCGTTGGAGATTTTACTATTTTTGCAGTTTATGGCTACATATGAATTGCTTTCTACCATAAATGGAGGTGACATTGTCAATCTTCTTGGGGTGATTTCTCGGCATAGACAGGCTCTAGAACTATGTCAGTTGGTTGGCTTTGCAGATAAGATTCCTGGTTTTATTTGGAATCTCATTGACAGGAAGCAACTAATTGATGCTGTTAGATGCATTTGCATCTTCAAGTTAATAGACAAGTTCCCTGCTGAGAAGGTTGTAGATGGTCTAATAGGTGATCTAAGAGCTGTGCATCAATGCATCAAAGACTACAATCTTGAGTCCGAATATCGATCTGCAGACATCGAAGTACAAGTAATTCAGCTGGGAAGACTGAAGGAGCATTACAGAAGTTTGGCGCCATCTCTTGCCTCCAGGGTCGATCAACAAGATCAGAGAAAAAGGAAGAGACCAAGCACCAGCACTTCTGCCCCCATCATTCAACCACATGAACAGCTTGAACAAAGTAATCACCAGACAGCTGTATCCACTGCTATACCCTATGCATTGCCGATTTCCACTTCAATTTGCCCAGAGTCAAGTTCGTTATCTAGGCTGTATGCAAATTATGGACTTCCTGGACAGTTTGGTATGGCAGCCAATGACCACGCAACCTGTGCTAATGCTGAAGGTGGCTTGATGCTAACATTGACAGGCCAATATGGATCGAATGTCCGAAATCCACTCCAAGTGCACAGTGCTGATCATGACCAATCTCATACAGTCATCCTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

939

Amino Acids

107.27

Weight (kDa)

7.06

Isoelectric Point (pI)

45.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	386 - 532	1.7e-26	Frigida-like protein
Frigida	PF07899	581 - 764	5.4e-38	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	125
AccB1I	GGYRCC	1 cut(s)	2437
AciI	CCGC	2 cut(s)	1434, 1457
AclI	AACGTT	1 cut(s)	952
AclWI	GGATC	3 cut(s)	1338, 1529, 2752
AcoI	YGGCCR	2 cut(s)	1680, 1806
AcsI	RAATTY	3 cut(s)	1297, 1592, 1744
AcuI	CTGAAG	4 cut(s)	95, 1659, 2435, 2727
AcyI	GRCGYC	1 cut(s)	2438
AfaI	GTAC	6 cut(s)	245, 479, 1514, 1791, 1931, 2391
AfiI	CCNNNNNNNGG	2 cut(s)	187, 1440
AflIII	ACRYGT	1 cut(s)	1926
AhlI	ACTAGT	1 cut(s)	290
AjnI	CCWGG	7 cut(s)	135, 180, 207, 1596, 2197, 2453, 2656
AjuI	GAANNNNNNNTTGG	2 cut(s)	1436, 1468
Alw21I	GWGCWC	2 cut(s)	180, 2775
Alw26I	GTCTC	8 cut(s)	29, 851, 895, 976, 1242, 1615, 1934, 2482
Alw44I	GTGCAC	1 cut(s)	2771
AlwI	GGATC	3 cut(s)	1338, 1529, 2752
Ama87I	CYCGRG	2 cut(s)	125, 186
AoxI	GGCC	6 cut(s)	183, 1599, 1680, 1806, 1970, 2734
ApaLI	GTGCAC	1 cut(s)	2771
ApeKI	GCWGC	3 cut(s)	1186, 1632, 2675
ApoI	RAATTY	3 cut(s)	1297, 1592, 1744
AseI	ATTAAT	1 cut(s)	1398
Asp700I	GAANNNNTTC	2 cut(s)	603, 1035
AspLEI	GCGC	1 cut(s)	2440
AspS9I	GGNCC	6 cut(s)	121, 130, 150, 184, 1600, 1970
AsuC2I	CCSGG	4 cut(s)	148, 166, 187, 188
AsuHPI	GGTGA	9 cut(s)	223, 460, 691, 1471, 1815, 2123, 2146, 2327, 2543
AvaI	CYCGRG	2 cut(s)	125, 186
AvaII	GGWCC	3 cut(s)	121, 130, 150
BaeGI	GKGCMC	1 cut(s)	2775
BaeI	ACNNNNGTAYC	2 cut(s)	2547, 2580
BalI	TGGCCA	2 cut(s)	1682, 1808
BanI	GGYRCC	1 cut(s)	2437
BanII	GRGCYC	1 cut(s)	180
BarI	GAAGNNNNNNTAC	2 cut(s)	2408, 2440
BauI	CACGAG	2 cut(s)	173, 1974
BbsI	GAAGAC	3 cut(s)	101, 1547, 2416
Bbv12I	GWGCWC	2 cut(s)	180, 2775
BbvI	GCAGC	3 cut(s)	1198, 1644, 2687
BccI	CCATC	5 cut(s)	610, 661, 2297, 2449, 2522
BceAI	ACGGC	1 cut(s)	1164
BciT130I	CCWGG	7 cut(s)	137, 182, 209, 1598, 2199, 2455, 2658
BciVI	GTATCC	2 cut(s)	1094, 2575
BclI	TGATCA	2 cut(s)	1176, 2782
BcnI	CCSGG	4 cut(s)	148, 166, 187, 188
BcoDI	GTCTC	8 cut(s)	29, 851, 895, 976, 1242, 1615, 1934, 2482
BcuI	ACTAGT	1 cut(s)	290
BfaI	CTAG	8 cut(s)	104, 291, 1080, 1097, 1161, 2019, 2159, 2631
BfmI	CTRYAG	3 cut(s)	1367, 1563, 2376
BfoI	RGCGCY	1 cut(s)	2441
BfuI	GTATCC	2 cut(s)	1094, 2575
BisI	GCNGC	4 cut(s)	1187, 1434, 1633, 2676
BlsI	GCNGC	4 cut(s)	1188, 1435, 1634, 2677
BmcAI	AGTACT	1 cut(s)	479
Bme18I	GGWCC	3 cut(s)	121, 130, 150
BmeT110I	CYCGRG	2 cut(s)	125, 186
BmgT120I	GGNCC	6 cut(s)	121, 130, 150, 184, 1600, 1970
BmiI	GGNNCC	3 cut(s)	170, 1665, 2439
BmrI	ACTGGG	1 cut(s)	1643
BmuI	ACTGGG	1 cut(s)	1643
BpiI	GAAGAC	3 cut(s)	101, 1547, 2416
BpmI	CTGGAG	4 cut(s)	164, 1071, 1958, 2437
Bpu10I	CCTNAGC	1 cut(s)	1404
BpuEI	CTTGAG	6 cut(s)	242, 386, 911, 1228, 1516, 2378
BpuMI	CCSGG	4 cut(s)	148, 166, 187, 188
Bsa29I	ATCGAT	2 cut(s)	614, 2371
BsaAI	YACGTR	1 cut(s)	1512
BsaBI	GATNNNNATC	1 cut(s)	1329
BsaHI	GRCGYC	1 cut(s)	2438
BsaI	GGTCTC	2 cut(s)	1242, 2482
BsaJI	CCNNGG	4 cut(s)	186, 779, 1603, 2454
BsaWI	WCCGGW	1 cut(s)	1666
BsaXI	ACNNNNNCTCC	8 cut(s)	227, 257, 656, 686, 704, 734, 776, 806
Bsc4I	CCNNNNNNNGG	2 cut(s)	187, 1440
Bse1I	ACTGG	3 cut(s)	1054, 1649, 1941
Bse3DI	GCAATG	3 cut(s)	714, 1281, 2585
Bse8I	GATNNNNATC	1 cut(s)	1329
BseBI	CCWGG	7 cut(s)	137, 182, 209, 1598, 2199, 2455, 2658
BseCI	ATCGAT	2 cut(s)	614, 2371
BseDI	CCNNGG	4 cut(s)	186, 779, 1603, 2454
BseGI	GGATG	4 cut(s)	699, 1090, 1276, 2805
BseJI	GATNNNNATC	1 cut(s)	1329
BseLI	CCNNNNNNNGG	2 cut(s)	187, 1440
BseMI	GCAATG	3 cut(s)	714, 1281, 2585
BseMII	CTCAG	8 cut(s)	44, 872, 1418, 1428, 1458, 1626, 1886, 2280
BseNI	ACTGG	3 cut(s)	1054, 1649, 1941
BseRI	GAGGAG	4 cut(s)	415, 425, 728, 994
BseSI	GKGCMC	1 cut(s)	2775
BseXI	GCAGC	3 cut(s)	1198, 1644, 2687
BseYI	CCCAGC	3 cut(s)	1252, 1831, 2404
BsgI	GTGCAG	1 cut(s)	2027
BshFI	GGCC	6 cut(s)	185, 1601, 1682, 1808, 1972, 2736
BshNI	GGYRCC	1 cut(s)	2437
BshVI	ATCGAT	2 cut(s)	614, 2371
BsiHKAI	GWGCWC	2 cut(s)	180, 2775
BsiHKCI	CYCGRG	2 cut(s)	125, 186
BsiSI	CCGG	4 cut(s)	148, 166, 187, 1667
BsiWI	CGTACG	1 cut(s)	1512
BslI	CCNNNNNNNGG	2 cut(s)	187, 1440
BsmAI	GTCTC	8 cut(s)	29, 851, 895, 976, 1242, 1615, 1934, 2482
BsmBI	CGTCTC	1 cut(s)	29
BsmI	GAATGC	1 cut(s)	366
BsnI	GGCC	6 cut(s)	185, 1601, 1682, 1808, 1972, 2736
Bso31I	GGTCTC	2 cut(s)	1242, 2482
BsoBI	CYCGRG	2 cut(s)	125, 186
Bsp1286I	GDGCHC	2 cut(s)	180, 2775
Bsp143I	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
BspACI	CCGC	2 cut(s)	1434, 1457
BspANI	GGCC	6 cut(s)	185, 1601, 1682, 1808, 1972, 2736
BspCNI	CTCAG	8 cut(s)	45, 871, 1417, 1429, 1459, 1625, 1885, 2281
BspDI	ATCGAT	2 cut(s)	614, 2371
BspHI	TCATGA	3 cut(s)	1179, 1914, 2785
BspLI	GGNNCC	3 cut(s)	170, 1665, 2439
BspMAI	CTGCAG	1 cut(s)	2380
BspPI	GGATC	3 cut(s)	1338, 1529, 2752
BspQI	GCTCTTC	2 cut(s)	291, 1236
BspT107I	GGYRCC	1 cut(s)	2437
BspTNI	GGTCTC	2 cut(s)	1242, 2482
BsrDI	GCAATG	3 cut(s)	714, 1281, 2585
BsrI	ACTGG	3 cut(s)	1054, 1649, 1941
BssECI	CCNNGG	4 cut(s)	186, 779, 1603, 2454
BssMI	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
BssNI	GRCGYC	1 cut(s)	2438
BssSI	CACGAG	2 cut(s)	173, 1974
BssT1I	CCWWGG	1 cut(s)	1603
Bst2BI	CACGAG	2 cut(s)	173, 1974
Bst2UI	CCWGG	7 cut(s)	137, 182, 209, 1598, 2199, 2455, 2658
Bst4CI	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2664, 2777, 2803
Bst6I	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2480
BstACI	GRCGYC	1 cut(s)	2438
BstAPI	GCANNNNNTGC	2 cut(s)	1157, 2696
BstBAI	YACGTR	1 cut(s)	1512
BstC8I	GCNNGC	3 cut(s)	1630, 2001, 2038
BstEII	GGTNACC	1 cut(s)	1459
BstF5I	GGATG	4 cut(s)	699, 1090, 1276, 2805
BstH2I	RGCGCY	1 cut(s)	2441
BstHHI	GCGC	1 cut(s)	2440
BstKTI	GATC	9 cut(s)	1179, 1333, 1537, 2320, 2375, 2464, 2473, 2747, 2785
BstMAI	GTCTC	8 cut(s)	29, 851, 895, 976, 1242, 1615, 1934, 2482
BstMBI	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
BstMWI	GCNNNNNNNGC	7 cut(s)	191, 200, 1157, 1454, 1969, 2005, 2696
BstNI	CCWGG	7 cut(s)	137, 182, 209, 1598, 2199, 2455, 2658
BstNSI	RCATGY	1 cut(s)	1930
BstPI	GGTNACC	1 cut(s)	1459
BstSFI	CTRYAG	3 cut(s)	1367, 1563, 2376
BstSLI	GKGCMC	1 cut(s)	2775
BstSNI	TACGTA	1 cut(s)	1512
BstV1I	GCAGC	3 cut(s)	1198, 1644, 2687
BstV2I	GAAGAC	3 cut(s)	101, 1547, 2416
BstX2I	RGATCY	1 cut(s)	1330
BstXI	CCANNNNNNTGG	1 cut(s)	1604
BstYI	RGATCY	1 cut(s)	1330
Bsu15I	ATCGAT	2 cut(s)	614, 2371
BsuI	GTATCC	2 cut(s)	1094, 2575
BsuRI	GGCC	6 cut(s)	185, 1601, 1682, 1808, 1972, 2736
BsuTUI	ATCGAT	2 cut(s)	614, 2371
BtsCI	GGATG	4 cut(s)	699, 1090, 1276, 2805
BtsI	GCAGTG	3 cut(s)	2010, 2015, 2568
BtsIMutI	CAGTG	9 cut(s)	119, 948, 1464, 1683, 1880, 2010, 2015, 2568, 2782
Cac8I	GCNNGC	3 cut(s)	1630, 2001, 2038
CciI	TCATGA	3 cut(s)	1179, 1914, 2785
CfoI	GCGC	1 cut(s)	2440
Cfr13I	GGNCC	6 cut(s)	121, 130, 150, 184, 1600, 1970
Cfr9I	CCCGGG	1 cut(s)	186
ClaI	ATCGAT	2 cut(s)	614, 2371
CsiI	ACCWGGT	1 cut(s)	207
Csp6I	GTAC	6 cut(s)	244, 478, 1513, 1790, 1930, 2390
CviAII	CATG	6 cut(s)	1180, 1528, 1915, 1927, 2528, 2786
CviQI	GTAC	6 cut(s)	244, 478, 1513, 1790, 1930, 2390
DinI	GGCGCC	1 cut(s)	2439
DpnI	GATC	9 cut(s)	1178, 1332, 1536, 2319, 2374, 2463, 2472, 2746, 2784
DpnII	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
DraI	TTTAAA	1 cut(s)	270
EaeI	YGGCCR	2 cut(s)	1680, 1806
Eam1104I	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2480
EarI	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2480
Ecl136II	GAGCTC	1 cut(s)	178
Eco105I	TACGTA	1 cut(s)	1512
Eco130I	CCWWGG	1 cut(s)	1603
Eco24I	GRGCYC	1 cut(s)	180
Eco31I	GGTCTC	2 cut(s)	1242, 2482
Eco47I	GGWCC	3 cut(s)	121, 130, 150
Eco53kI	GAGCTC	1 cut(s)	178
Eco57I	CTGAAG	4 cut(s)	95, 1659, 2435, 2727
Eco88I	CYCGRG	2 cut(s)	125, 186
Eco91I	GGTNACC	1 cut(s)	1459
EcoICRI	GAGCTC	1 cut(s)	178
EcoO109I	RGGNCCY	2 cut(s)	121, 1600
EcoO65I	GGTNACC	1 cut(s)	1459
EcoRI	GAATTC	1 cut(s)	1592
EcoRII	CCWGG	7 cut(s)	135, 180, 207, 1596, 2197, 2453, 2656
EcoT14I	CCWWGG	1 cut(s)	1603
EcoT22I	ATGCAT	4 cut(s)	699, 2255, 2342, 2587
EcoT38I	GRGCYC	1 cut(s)	180
EgeI	GGCGCC	1 cut(s)	2439
EheI	GGCGCC	1 cut(s)	2439
ErhI	CCWWGG	1 cut(s)	1603
Esp3I	CGTCTC	1 cut(s)	29
FaeI	CATG	6 cut(s)	1183, 1531, 1918, 1930, 2531, 2789
FalI	AAGNNNNNCTT	2 cut(s)	242, 274
FatI	CATG	6 cut(s)	1179, 1527, 1914, 1926, 2527, 2785
FauNDI	CATATG	2 cut(s)	1113, 2083
FbaI	TGATCA	2 cut(s)	1176, 2782
Fnu4HI	GCNGC	4 cut(s)	1187, 1434, 1633, 2676
FokI	GGATG	4 cut(s)	706, 1077, 1283, 2792
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	4 cut(s)	1187, 1434, 1633, 2676
FspBI	CTAG	8 cut(s)	104, 291, 1080, 1097, 1161, 2019, 2159, 2631
GlaI	GCGC	1 cut(s)	2439
GluI	GCNGC	4 cut(s)	1187, 1434, 1633, 2676
GsaI	CCCAGC	3 cut(s)	1256, 1835, 2408
GsuI	CTGGAG	4 cut(s)	164, 1071, 1958, 2437
HaeII	RGCGCY	1 cut(s)	2441
HaeIII	GGCC	6 cut(s)	185, 1601, 1682, 1808, 1972, 2736
HapII	CCGG	4 cut(s)	148, 166, 187, 1667
HhaI	GCGC	1 cut(s)	2440
Hin1I	GRCGYC	1 cut(s)	2438
Hin1II	CATG	6 cut(s)	1183, 1531, 1918, 1930, 2531, 2789
Hin6I	GCGC	1 cut(s)	2438
HinP1I	GCGC	1 cut(s)	2438
HincII	GTYRAC	1 cut(s)	864
HindII	GTYRAC	1 cut(s)	864
HindIII	AAGCTT	2 cut(s)	84, 218
HpaII	CCGG	4 cut(s)	148, 166, 187, 1667
HphI	GGTGA	9 cut(s)	223, 460, 691, 1471, 1815, 2123, 2146, 2327, 2543
Hpy166II	GTNNAC	3 cut(s)	864, 1309, 2773
Hpy188III	TCNNGA	9 cut(s)	160, 959, 1180, 1245, 1495, 1915, 2159, 2357, 2786
Hpy8I	GTNNAC	3 cut(s)	864, 1309, 2773
Hpy99I	CGWCG	1 cut(s)	1139
HpyCH4III	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2664, 2777, 2803
HpyCH4IV	ACGT	2 cut(s)	952, 1511
HpyF10VI	GCNNNNNNNGC	7 cut(s)	191, 200, 1157, 1454, 1969, 2005, 2696
HpySE526I	ACGT	2 cut(s)	952, 1511
Hsp92I	GRCGYC	1 cut(s)	2438
Hsp92II	CATG	6 cut(s)	1183, 1531, 1918, 1930, 2531, 2789
HspAI	GCGC	1 cut(s)	2438
KasI	GGCGCC	1 cut(s)	2437
Ksp22I	TGATCA	2 cut(s)	1176, 2782
Kzo9I	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
LguI	GCTCTTC	2 cut(s)	291, 1236
LmnI	GCTCC	4 cut(s)	183, 1318, 1543, 2419
Lsp1109I	GCAGC	3 cut(s)	1198, 1644, 2687
MabI	ACCWGGT	1 cut(s)	207
MaeI	CTAG	8 cut(s)	104, 291, 1080, 1097, 1161, 2019, 2159, 2631
MaeII	ACGT	2 cut(s)	952, 1511
MaeIII	GTNAC	6 cut(s)	254, 1459, 1504, 1906, 1922, 2111
MalI	GATC	9 cut(s)	1178, 1332, 1536, 2319, 2374, 2463, 2472, 2746, 2784
MboI	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	2 cut(s)	180, 2775
MlsI	TGGCCA	2 cut(s)	1682, 1808
MluNI	TGGCCA	2 cut(s)	1682, 1808
Mly113I	GGCGCC	1 cut(s)	2438
MlyI	GAGTC	6 cut(s)	134, 233, 262, 1964, 2369, 2624
MmeI	TCCRAC	5 cut(s)	450, 1458, 1827, 1859, 2028
Mox20I	TGGCCA	2 cut(s)	1682, 1808
Mph1103I	ATGCAT	4 cut(s)	699, 2255, 2342, 2587
MroXI	GAANNNNTTC	2 cut(s)	603, 1035
MscI	TGGCCA	2 cut(s)	1682, 1808
MseI	TTAA	9 cut(s)	269, 278, 534, 639, 837, 1208, 1376, 1398, 2270
Msp20I	TGGCCA	2 cut(s)	1682, 1808
MspA1I	CMGCKG	3 cut(s)	1436, 2404, 2561
MspI	CCGG	4 cut(s)	148, 166, 187, 1667
Mva1269I	GAATGC	1 cut(s)	366
MvaI	CCWGG	7 cut(s)	137, 182, 209, 1598, 2199, 2455, 2658
MwoI	GCNNNNNNNGC	7 cut(s)	191, 200, 1157, 1454, 1969, 2005, 2696
NarI	GGCGCC	1 cut(s)	2438
NciI	CCSGG	4 cut(s)	148, 166, 187, 188
NdeI	CATATG	2 cut(s)	1113, 2083
NdeII	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
NlaIII	CATG	6 cut(s)	1183, 1531, 1918, 1930, 2531, 2789
NlaIV	GGNNCC	3 cut(s)	170, 1665, 2439
NmeAIII	GCCGAG	3 cut(s)	804, 825, 2122
NmuCI	GTSAC	3 cut(s)	254, 1459, 2111
NsiI	ATGCAT	4 cut(s)	699, 2255, 2342, 2587
NspI	RCATGY	1 cut(s)	1930
PaeR7I	CTCGAG	1 cut(s)	125
PagI	TCATGA	3 cut(s)	1179, 1914, 2785
PciI	ACATGT	1 cut(s)	1926
PciSI	GCTCTTC	2 cut(s)	291, 1236
PctI	GAATGC	1 cut(s)	366
PdmI	GAANNNNTTC	2 cut(s)	603, 1035
PfeI	GAWTC	7 cut(s)	604, 1031, 1325, 1383, 1670, 2194, 2212
Pfl23II	CGTACG	1 cut(s)	1512
PflFI	GACNNNGTC	1 cut(s)	2117
PfoI	TCCNGGA	1 cut(s)	2656
PkrI	GCNGC	4 cut(s)	1188, 1435, 1634, 2677
PleI	GAGTC	6 cut(s)	134, 232, 261, 1963, 2368, 2623
PluTI	GGCGCC	1 cut(s)	2441
PpsI	GAGTC	6 cut(s)	134, 232, 261, 1963, 2368, 2623
Ppu21I	YACGTR	1 cut(s)	1512
PpuMI	RGGWCCY	1 cut(s)	121
PscI	ACATGT	1 cut(s)	1926
PshBI	ATTAAT	1 cut(s)	1398
Psp124BI	GAGCTC	1 cut(s)	180
Psp1406I	AACGTT	1 cut(s)	952
Psp5II	RGGWCCY	1 cut(s)	121
Psp6I	CCWGG	7 cut(s)	135, 180, 207, 1596, 2197, 2453, 2656
PspEI	GGTNACC	1 cut(s)	1459
PspFI	CCCAGC	3 cut(s)	1252, 1831, 2404
PspGI	CCWGG	7 cut(s)	135, 180, 207, 1596, 2197, 2453, 2656
PspLI	CGTACG	1 cut(s)	1512
PspN4I	GGNNCC	3 cut(s)	170, 1665, 2439
PspPI	GGNCC	6 cut(s)	121, 130, 150, 184, 1600, 1970
PspPPI	RGGWCCY	1 cut(s)	121
PspXI	VCTCGAGB	1 cut(s)	125
PstI	CTGCAG	1 cut(s)	2380
PsuI	RGATCY	1 cut(s)	1330
PsyI	GACNNNGTC	1 cut(s)	2117
PvuII	CAGCTG	2 cut(s)	2404, 2561
RsaI	GTAC	6 cut(s)	245, 479, 1514, 1791, 1931, 2391
RsaNI	GTAC	6 cut(s)	244, 478, 1513, 1790, 1930, 2390
SacI	GAGCTC	1 cut(s)	180
SapI	GCTCTTC	2 cut(s)	291, 1236
SaqAI	TTAA	9 cut(s)	269, 278, 534, 639, 837, 1208, 1376, 1398, 2270
SatI	GCNGC	4 cut(s)	1187, 1434, 1633, 2676
Sau3AI	GATC	9 cut(s)	1176, 1330, 1534, 2317, 2372, 2461, 2470, 2744, 2782
Sau96I	GGNCC	6 cut(s)	121, 130, 150, 184, 1600, 1970
ScaI	AGTACT	1 cut(s)	479
SchI	GAGTC	6 cut(s)	134, 233, 262, 1964, 2369, 2624
SduI	GDGCHC	2 cut(s)	180, 2775
SexAI	ACCWGGT	1 cut(s)	207
SfcI	CTRYAG	3 cut(s)	1367, 1563, 2376
SfoI	GGCGCC	1 cut(s)	2439
Sfr274I	CTCGAG	1 cut(s)	125
SinI	GGWCC	3 cut(s)	121, 130, 150
SlaI	CTCGAG	1 cut(s)	125
SmaI	CCCGGG	1 cut(s)	188
SmlI	CTYRAG	7 cut(s)	125, 221, 365, 890, 1243, 1495, 2357
SmoI	CTYRAG	7 cut(s)	125, 221, 365, 890, 1243, 1495, 2357
SnaBI	TACGTA	1 cut(s)	1512
SpeI	ACTAGT	1 cut(s)	290
SsiI	CCGC	2 cut(s)	1434, 1457
SspDI	GGCGCC	1 cut(s)	2437
SspI	AATATT	1 cut(s)	1105
SspMI	CTAG	8 cut(s)	104, 291, 1080, 1097, 1161, 2019, 2159, 2631
SstI	GAGCTC	1 cut(s)	180
StyI	CCWWGG	1 cut(s)	1603
TaaI	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2664, 2777, 2803
TaiI	ACGT	2 cut(s)	955, 1514
TaqI	TCGA	9 cut(s)	126, 143, 614, 644, 1067, 2371, 2385, 2460, 2747
TatI	WGTACW	3 cut(s)	477, 1789, 2389
TauI	GCSGC	1 cut(s)	1436
TfiI	GAWTC	7 cut(s)	604, 1031, 1325, 1383, 1670, 2194, 2212
Tru1I	TTAA	9 cut(s)	269, 278, 534, 639, 837, 1208, 1376, 1398, 2270
Tru9I	TTAA	9 cut(s)	269, 278, 534, 639, 837, 1208, 1376, 1398, 2270
TscAI	CASTG	9 cut(s)	119, 948, 1471, 1683, 1880, 2010, 2015, 2575, 2782
TseFI	GTSAC	3 cut(s)	254, 1459, 2111
TseI	GCWGC	3 cut(s)	1186, 1632, 2675
Tsp45I	GTSAC	3 cut(s)	254, 1459, 2111
TspDTI	ATGAA	9 cut(s)	855, 986, 1076, 1079, 1692, 1747, 1931, 2100, 2544
TspMI	CCCGGG	1 cut(s)	186
TspRI	CASTG	9 cut(s)	119, 948, 1471, 1683, 1880, 2010, 2015, 2575, 2782
Tth111I	GACNNNGTC	1 cut(s)	2117
VneI	GTGCAC	1 cut(s)	2771
VpaK11BI	GGWCC	3 cut(s)	121, 130, 150
VspI	ATTAAT	1 cut(s)	1398
XapI	RAATTY	3 cut(s)	1297, 1592, 1744
XbaI	TCTAGA	1 cut(s)	2158
XceI	RCATGY	1 cut(s)	1930
XhoI	CTCGAG	1 cut(s)	125
XmaI	CCCGGG	1 cut(s)	186
XmnI	GAANNNNTTC	2 cut(s)	603, 1035
XspI	CTAG	8 cut(s)	104, 291, 1080, 1097, 1161, 2019, 2159, 2631
ZrmI	AGTACT	1 cut(s)	479
Zsp2I	ATGCAT	4 cut(s)	699, 2255, 2342, 2587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.