Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Forward (+)

50305039 .. 50310076

Gene Structure 5038 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00430100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGGAGTTGAAGGTGAAGGAGTTTGGGATGCATAAGAAGGCAATGGAGGAGTGGTGTTGTAAGGTTGAAGTGAAGAAGAGGGAACTTGAAGTGTTGGTGGAGAAGGTTGAGCCGAGGGAGAGAGAGTTTGAGCCGAGAGTTGAGGAACTTAATTCGATTGGTAAGAGGGTTAATGAATGTCTCAATGAGGCTCAGTTGACATTGGAAAGGGATTTTCGTTTGCTTGAGGAAATGAGACAGGAGAATGTGAAGCATTTTGAGTTAGTTGAGAAGTCAGTGCAAGAACGTTCTCATGAACTTGAAATGAAAGAGAGGAGACTTGAAGAAAAGGCAAAAGAGCTTGACTTGAAACAAAAACTATTGGAATCTATTCCGAAAGCTACTGGAGAACAAATGAAATCAAAGGAGAAGGCTAGTATCCTTCATCCTCTAGTGAATATTCCACATATGTTCCCTGCAAACAATGTCGTTGTTCCTTCAACAGCAAGTAATGCTAGAGGTTTTCAGTTGATCGTGAGTGAGCATTTGAAGAGAATGGATTTAATGAGTAGAGAAATATCAGCTCTTCTTCAAGCCTCAAGAGACCCAGCAGGATTGGTTTTGGATGCAATGCAGGGGTTTTACCCTACAAATTCAACTGGGGACAACAGGGAGCTTGATTCGGATCTAAGAGTGATTAGAAGGAGTTGTATTGTATTGCTACAGGAGTTAAAGAGATTCTCGCCACAAATTAATGCTCAGGTCAGAGAAAAGGCAATGAAGTTAGCAGCTGAGTGGAAGGCTAAGTTGGCGGTGACCACTGAGAATAGGTTGGAGGTGTTGGGCTTCTTGCGGCTTGTTACTACGTATGAATTGACCTCCATGTATGATCCAAAGGAGCTTCACAGTCTTCTCTCAATAGTTGTTCAGCCCAAACAAACAACTGAATTCCAGGCCCTTGGTGTCTCAGATAAGGCATTTGCGAGCAGCACAATTTCTTTCCCAGTTAGAATTGAGGAACCGGAATCTGCAGTGGCCAAATGTGTAGCCCCATTTTCATCTCCAAATCTTCAACTAAGTGCAACCAGAGAACCAACAAATTTTCAGGGGTTCATAGTTGAGCGTTTGAGTGAGAATAATTCAGTACAGGAAAAAATGTTGGCCACTCTTCAAGTGTCACCAGACCCAGCACAAATTGTGTTGGAAATGATGCAAAGCTCTTTTGCTCAGTGTTGGAGAGAAGGAGGTTTTTTTTCAGAAGTAACTGTCATGAAGGGTTACATTTACCTACTGGAGACACTAATGAGAGAGTCAAAGCACATTGGACCTCGTGTGAAAGAAGATGCAAGAAAGCTGGCAGTGCAGTGGAAAGCTAGAATGAGAGCCGATGCTGGCAACTCGTTGGAGATTTTACTATTTTTGCAGTTTATGGCTACATATGAATTGCTCTCTACCATAAATGGAGGTGACATTGTCAATCTTCTTGGGGTGATTTCTCGGCATAGACAGGCTCTAGAACTATGTCAGTCGGTTGGCTTTGCAGATAAGATTCCTGGTTTTATTTGGAATCTCATTGACAGGAAGCAACTAATTGATGCTGCTAGATGCATTTGCATCTTCAAGTTAATAGACAAGTTCCCTGCCGTACAACTTTTGAAAGATCATGTGCATAATGCAAGAAAGCATGCCAGTGAAATTTGCAAAAATCAGTTATCATTTGGTGAAACGGAGAAGGTTGTAGATGGTCTAATAGGTGATCTAAGAGCTGTGCATCAATGCATCAAAGACTACGATCTTGAGTCCGAATATCCACCTGCAGACATCGAAGTACAAGTAATTCAGCTGGGAAGAGTGAAGGAGCATTACAGAAGTTTGGCGCCATCTCTGGCCTCTGGGGTCGATCAACAAGATCGGAGGAAAAGGAAGAGACCAAGCACCAGCACTTCTGCCCCCATCATTCAACCACATGAACAGCTTGAACAAAGTAATCACCAGACAGCTGTATCCACTGCTATACCCCATGCATTGCCGATTTCCACTTCAATTTGTCCAGAGTCAAGTTCGCTATCTAGGCTGTATGCAAATTATGGACTCCCTGGACAGTTTGGTATGACAGCCAATGACCACGCAACCTGTGCTAATTCTGAAGGTGGCTTGATGCTAACATTGACAGGCCAATATGGATCGAATGTCAGAAATCCACTCCAAGTGCACAGTGCTGATCATGACCATGCTCATACAGTCATCCTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

744

Amino Acids

84.08

Weight (kDa)

6.46

Isoelectric Point (pI)

45.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	164 - 310	1.1e-29	Frigida-like protein
Frigida	PF07899	359 - 614	1e-55	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1801
Acc36I	ACCTGC	1 cut(s)	1801
AccB1I	GGYRCC	1 cut(s)	1855
AciI	CCGC	2 cut(s)	791, 832
AclI	AACGTT	1 cut(s)	286
AclWI	GGATC	3 cut(s)	672, 863, 2170
AcoI	YGGCCR	2 cut(s)	1014, 1140
AcsI	RAATTY	4 cut(s)	631, 926, 1078, 1674
AcuI	CTGAAG	1 cut(s)	2145
AcyI	GRCGYC	1 cut(s)	1856
AfaI	GTAC	3 cut(s)	1125, 1626, 1809
AjnI	CCWGG	3 cut(s)	930, 1531, 2074
AjuI	GAANNNNNNNTTGG	2 cut(s)	770, 802
Alw21I	GWGCWC	1 cut(s)	2193
Alw26I	GTCTC	7 cut(s)	185, 229, 310, 576, 949, 1268, 1900
Alw44I	GTGCAC	1 cut(s)	2189
AlwI	GGATC	3 cut(s)	672, 863, 2170
AoxI	GGCC	5 cut(s)	933, 1014, 1140, 1866, 2152
ApaLI	GTGCAC	1 cut(s)	2189
ApeKI	GCWGC	3 cut(s)	767, 966, 1577
ApoI	RAATTY	4 cut(s)	631, 926, 1078, 1674
AseI	ATTAAT	1 cut(s)	732
Asp700I	GAANNNNTTC	1 cut(s)	369
AspLEI	GCGC	1 cut(s)	1858
AspS9I	GGNCC	2 cut(s)	934, 1304
AsuHPI	GGTGA	8 cut(s)	25, 805, 1149, 1457, 1480, 1712, 1745, 1961
AvaII	GGWCC	1 cut(s)	1304
BaeGI	GKGCMC	1 cut(s)	2193
BaeI	ACNNNNGTAYC	2 cut(s)	1965, 1998
BalI	TGGCCA	2 cut(s)	1016, 1142
BanI	GGYRCC	1 cut(s)	1855
BarI	GAAGNNNNNNTAC	2 cut(s)	1826, 1858
BauI	CACGAG	1 cut(s)	1308
BbsI	GAAGAC	1 cut(s)	881
Bbv12I	GWGCWC	1 cut(s)	2193
BbvI	GCAGC	3 cut(s)	779, 978, 1564
BccI	CCATC	3 cut(s)	1715, 1867, 1940
BceAI	ACGGC	1 cut(s)	1607
BciT130I	CCWGG	3 cut(s)	932, 1533, 2076
BciVI	GTATCC	2 cut(s)	428, 1993
BclI	TGATCA	1 cut(s)	2200
BcoDI	GTCTC	7 cut(s)	185, 229, 310, 576, 949, 1268, 1900
BfaI	CTAG	7 cut(s)	414, 431, 495, 1353, 1493, 1581, 2049
BfmI	CTRYAG	3 cut(s)	701, 1008, 1794
BfoI	RGCGCY	1 cut(s)	1859
BfuAI	ACCTGC	1 cut(s)	1801
BfuI	GTATCC	2 cut(s)	428, 1993
BisI	GCNGC	4 cut(s)	768, 833, 967, 1578
BlsI	GCNGC	4 cut(s)	769, 834, 968, 1579
Bme1390I	CCNGG	3 cut(s)	932, 1533, 2076
Bme18I	GGWCC	1 cut(s)	1304
BmgT120I	GGNCC	2 cut(s)	934, 1304
BmiI	GGNNCC	2 cut(s)	999, 1857
BmrFI	CCNGG	3 cut(s)	932, 1533, 2076
BmrI	ACTGGG	2 cut(s)	648, 977
BmuI	ACTGGG	2 cut(s)	648, 977
BpiI	GAAGAC	1 cut(s)	881
BpmI	CTGGAG	2 cut(s)	405, 1292
Bpu10I	CCTNAGC	1 cut(s)	738
BpuEI	CTTGAG	3 cut(s)	245, 562, 1796
BsaAI	YACGTR	1 cut(s)	846
BsaBI	GATNNNNATC	1 cut(s)	663
BsaHI	GRCGYC	1 cut(s)	1856
BsaI	GGTCTC	2 cut(s)	576, 1900
BsaJI	CCNNGG	3 cut(s)	113, 937, 2074
BsaWI	WCCGGW	1 cut(s)	1000
BsaXI	ACNNNNNCTCC	4 cut(s)	38, 68, 110, 140
Bse1I	ACTGG	5 cut(s)	388, 643, 983, 1275, 1668
Bse3DI	GCAATG	4 cut(s)	48, 615, 762, 2003
Bse8I	GATNNNNATC	1 cut(s)	663
BseBI	CCWGG	3 cut(s)	932, 1533, 2076
BseDI	CCNNGG	3 cut(s)	113, 937, 2074
BseGI	GGATG	4 cut(s)	33, 424, 610, 2223
BseJI	GATNNNNATC	1 cut(s)	663
BseMI	GCAATG	4 cut(s)	48, 615, 762, 2003
BseMII	CTCAG	6 cut(s)	206, 752, 762, 792, 960, 1220
BseNI	ACTGG	5 cut(s)	388, 643, 983, 1275, 1668
BseRI	GAGGAG	2 cut(s)	62, 328
BseSI	GKGCMC	1 cut(s)	2193
BseXI	GCAGC	3 cut(s)	779, 978, 1564
BseYI	CCCAGC	3 cut(s)	586, 1165, 1822
BsgI	GTGCAG	1 cut(s)	1361
BshFI	GGCC	5 cut(s)	935, 1016, 1142, 1868, 2154
BshNI	GGYRCC	1 cut(s)	1855
BsiHKAI	GWGCWC	1 cut(s)	2193
BsiSI	CCGG	1 cut(s)	1001
BslFI	GGGAC	1 cut(s)	656
BsmAI	GTCTC	7 cut(s)	185, 229, 310, 576, 949, 1268, 1900
BsmFI	GGGAC	1 cut(s)	656
BsnI	GGCC	5 cut(s)	935, 1016, 1142, 1868, 2154
Bso31I	GGTCTC	2 cut(s)	576, 1900
Bsp1286I	GDGCHC	1 cut(s)	2193
BspACI	CCGC	2 cut(s)	791, 832
BspANI	GGCC	5 cut(s)	935, 1016, 1142, 1868, 2154
BspCNI	CTCAG	6 cut(s)	205, 751, 763, 793, 959, 1219
BspHI	TCATGA	3 cut(s)	292, 1248, 2203
BspLI	GGNNCC	2 cut(s)	999, 1857
BspMAI	CTGCAG	2 cut(s)	1012, 1798
BspMI	ACCTGC	1 cut(s)	1801
BspPI	GGATC	3 cut(s)	672, 863, 2170
BspQI	GCTCTTC	1 cut(s)	570
BspT107I	GGYRCC	1 cut(s)	1855
BspTNI	GGTCTC	2 cut(s)	576, 1900
BsrDI	GCAATG	4 cut(s)	48, 615, 762, 2003
BsrI	ACTGG	5 cut(s)	388, 643, 983, 1275, 1668
BssECI	CCNNGG	3 cut(s)	113, 937, 2074
BssNI	GRCGYC	1 cut(s)	1856
BssSI	CACGAG	1 cut(s)	1308
BssT1I	CCWWGG	1 cut(s)	937
Bst2BI	CACGAG	1 cut(s)	1308
Bst2UI	CCWGG	3 cut(s)	932, 1533, 2076
Bst4CI	ACNGT	5 cut(s)	887, 1246, 2082, 2195, 2221
Bst6I	CTCTTC	6 cut(s)	71, 524, 570, 1152, 1822, 1898
BstACI	GRCGYC	1 cut(s)	1856
BstAPI	GCANNNNNTGC	2 cut(s)	491, 2114
BstBAI	YACGTR	1 cut(s)	846
BstC8I	GCNNGC	4 cut(s)	964, 1335, 1372, 1665
BstEII	GGTNACC	1 cut(s)	793
BstF5I	GGATG	4 cut(s)	33, 424, 610, 2223
BstH2I	RGCGCY	1 cut(s)	1859
BstHHI	GCGC	1 cut(s)	1858
BstMAI	GTCTC	7 cut(s)	185, 229, 310, 576, 949, 1268, 1900
BstMWI	GCNNNNNNNGC	5 cut(s)	491, 788, 1339, 2050, 2114
BstNI	CCWGG	3 cut(s)	932, 1533, 2076
BstNSI	RCATGY	1 cut(s)	1667
BstPI	GGTNACC	1 cut(s)	793
BstSCI	CCNGG	3 cut(s)	930, 1531, 2074
BstSFI	CTRYAG	3 cut(s)	701, 1008, 1794
BstSLI	GKGCMC	1 cut(s)	2193
BstSNI	TACGTA	1 cut(s)	846
BstV1I	GCAGC	3 cut(s)	779, 978, 1564
BstV2I	GAAGAC	1 cut(s)	881
BstX2I	RGATCY	1 cut(s)	664
BstXI	CCANNNNNNTGG	1 cut(s)	938
BstYI	RGATCY	1 cut(s)	664
BsuI	GTATCC	2 cut(s)	428, 1993
BsuRI	GGCC	5 cut(s)	935, 1016, 1142, 1868, 2154
BtsCI	GGATG	4 cut(s)	33, 424, 610, 2223
BtsI	GCAGTG	4 cut(s)	1017, 1344, 1349, 1986
BtsIMutI	CAGTG	9 cut(s)	282, 798, 1017, 1214, 1344, 1349, 1675, 1986, 2200
BveI	ACCTGC	1 cut(s)	1801
Cac8I	GCNNGC	4 cut(s)	964, 1335, 1372, 1665
CciI	TCATGA	3 cut(s)	292, 1248, 2203
CfoI	GCGC	1 cut(s)	1858
Cfr13I	GGNCC	2 cut(s)	934, 1304
Csp6I	GTAC	3 cut(s)	1124, 1625, 1808
CviAII	CATG	9 cut(s)	293, 862, 1249, 1643, 1664, 1946, 2000, 2204, 2210
CviQI	GTAC	3 cut(s)	1124, 1625, 1808
DinI	GGCGCC	1 cut(s)	1857
EaeI	YGGCCR	2 cut(s)	1014, 1140
Eam1104I	CTCTTC	6 cut(s)	71, 524, 570, 1152, 1822, 1898
EarI	CTCTTC	6 cut(s)	71, 524, 570, 1152, 1822, 1898
Eco105I	TACGTA	1 cut(s)	846
Eco130I	CCWWGG	1 cut(s)	937
Eco31I	GGTCTC	2 cut(s)	576, 1900
Eco47I	GGWCC	1 cut(s)	1304
Eco57I	CTGAAG	1 cut(s)	2145
Eco91I	GGTNACC	1 cut(s)	793
EcoO109I	RGGNCCY	1 cut(s)	934
EcoO65I	GGTNACC	1 cut(s)	793
EcoRI	GAATTC	1 cut(s)	926
EcoRII	CCWGG	3 cut(s)	930, 1531, 2074
EcoT14I	CCWWGG	1 cut(s)	937
EcoT22I	ATGCAT	4 cut(s)	33, 1589, 1760, 2005
EgeI	GGCGCC	1 cut(s)	1857
EheI	GGCGCC	1 cut(s)	1857
ErhI	CCWWGG	1 cut(s)	937
FaeI	CATG	9 cut(s)	296, 865, 1252, 1646, 1667, 1949, 2003, 2207, 2213
FaqI	GGGAC	1 cut(s)	656
FatI	CATG	9 cut(s)	292, 861, 1248, 1642, 1663, 1945, 1999, 2203, 2209
FauNDI	CATATG	2 cut(s)	447, 1417
FbaI	TGATCA	1 cut(s)	2200
Fnu4HI	GCNGC	4 cut(s)	768, 833, 967, 1578
FokI	GGATG	4 cut(s)	40, 411, 617, 2210
Fsp4HI	GCNGC	4 cut(s)	768, 833, 967, 1578
FspBI	CTAG	7 cut(s)	414, 431, 495, 1353, 1493, 1581, 2049
GlaI	GCGC	1 cut(s)	1857
GluI	GCNGC	4 cut(s)	768, 833, 967, 1578
GsaI	CCCAGC	3 cut(s)	590, 1169, 1826
GsuI	CTGGAG	2 cut(s)	405, 1292
HaeII	RGCGCY	1 cut(s)	1859
HaeIII	GGCC	5 cut(s)	935, 1016, 1142, 1868, 2154
HapII	CCGG	1 cut(s)	1001
HhaI	GCGC	1 cut(s)	1858
Hin1I	GRCGYC	1 cut(s)	1856
Hin1II	CATG	9 cut(s)	296, 865, 1252, 1646, 1667, 1949, 2003, 2207, 2213
Hin6I	GCGC	1 cut(s)	1856
HinP1I	GCGC	1 cut(s)	1856
HincII	GTYRAC	1 cut(s)	198
HindII	GTYRAC	1 cut(s)	198
HpaII	CCGG	1 cut(s)	1001
HphI	GGTGA	8 cut(s)	25, 805, 1149, 1457, 1480, 1712, 1745, 1961
Hpy166II	GTNNAC	2 cut(s)	198, 2191
Hpy188I	TCNGA	9 cut(s)	375, 664, 746, 949, 1237, 1783, 1893, 2125, 2174
Hpy188III	TCNNGA	8 cut(s)	293, 514, 579, 1249, 1493, 1775, 2030, 2204
Hpy8I	GTNNAC	2 cut(s)	198, 2191
HpyCH4III	ACNGT	5 cut(s)	887, 1246, 2082, 2195, 2221
HpyCH4IV	ACGT	2 cut(s)	286, 845
HpyF10VI	GCNNNNNNNGC	5 cut(s)	491, 788, 1339, 2050, 2114
HpySE526I	ACGT	2 cut(s)	286, 845
Hsp92I	GRCGYC	1 cut(s)	1856
Hsp92II	CATG	9 cut(s)	296, 865, 1252, 1646, 1667, 1949, 2003, 2207, 2213
HspAI	GCGC	1 cut(s)	1856
KasI	GGCGCC	1 cut(s)	1855
Ksp22I	TGATCA	1 cut(s)	2200
LguI	GCTCTTC	1 cut(s)	570
LmnI	GCTCC	3 cut(s)	652, 877, 1837
Lsp1109I	GCAGC	3 cut(s)	779, 978, 1564
MaeI	CTAG	7 cut(s)	414, 431, 495, 1353, 1493, 1581, 2049
MaeII	ACGT	2 cut(s)	286, 845
MaeIII	GTNAC	6 cut(s)	793, 838, 1155, 1240, 1256, 1445
MflI	RGATCY	1 cut(s)	664
MhlI	GDGCHC	1 cut(s)	2193
MlsI	TGGCCA	2 cut(s)	1016, 1142
MluNI	TGGCCA	2 cut(s)	1016, 1142
Mly113I	GGCGCC	1 cut(s)	1856
MlyI	GAGTC	4 cut(s)	1298, 1787, 2042, 2064
MmeI	TCCRAC	4 cut(s)	792, 1161, 1193, 1362
Mox20I	TGGCCA	2 cut(s)	1016, 1142
Mph1103I	ATGCAT	4 cut(s)	33, 1589, 1760, 2005
MroXI	GAANNNNTTC	1 cut(s)	369
MscI	TGGCCA	2 cut(s)	1016, 1142
MseI	TTAA	6 cut(s)	150, 171, 542, 710, 732, 1604
MslI	CAYNNNNRTG	2 cut(s)	1668, 2208
Msp20I	TGGCCA	2 cut(s)	1016, 1142
MspA1I	CMGCKG	3 cut(s)	770, 1822, 1979
MspI	CCGG	1 cut(s)	1001
MspR9I	CCNGG	3 cut(s)	932, 1533, 2076
MvaI	CCWGG	3 cut(s)	932, 1533, 2076
MwoI	GCNNNNNNNGC	5 cut(s)	491, 788, 1339, 2050, 2114
NarI	GGCGCC	1 cut(s)	1856
NdeI	CATATG	2 cut(s)	447, 1417
NlaIII	CATG	9 cut(s)	296, 865, 1252, 1646, 1667, 1949, 2003, 2207, 2213
NlaIV	GGNNCC	2 cut(s)	999, 1857
NmeAIII	GCCGAG	3 cut(s)	138, 159, 1456
NmuCI	GTSAC	3 cut(s)	793, 1155, 1445
NsiI	ATGCAT	4 cut(s)	33, 1589, 1760, 2005
NspI	RCATGY	1 cut(s)	1667
PaeI	GCATGC	1 cut(s)	1667
PagI	TCATGA	3 cut(s)	292, 1248, 2203
PaqCI	CACCTGC	1 cut(s)	1801
PciSI	GCTCTTC	1 cut(s)	570
PdmI	GAANNNNTTC	1 cut(s)	369
PfeI	GAWTC	6 cut(s)	365, 659, 717, 1004, 1528, 1546
PflFI	GACNNNGTC	1 cut(s)	1451
PkrI	GCNGC	4 cut(s)	769, 834, 968, 1579
PleI	GAGTC	4 cut(s)	1297, 1786, 2041, 2064
PluTI	GGCGCC	1 cut(s)	1859
PpsI	GAGTC	4 cut(s)	1297, 1786, 2041, 2064
Ppu21I	YACGTR	1 cut(s)	846
PshBI	ATTAAT	1 cut(s)	732
Psp1406I	AACGTT	1 cut(s)	286
Psp6I	CCWGG	3 cut(s)	930, 1531, 2074
PspEI	GGTNACC	1 cut(s)	793
PspFI	CCCAGC	3 cut(s)	586, 1165, 1822
PspGI	CCWGG	3 cut(s)	930, 1531, 2074
PspN4I	GGNNCC	2 cut(s)	999, 1857
PspPI	GGNCC	2 cut(s)	934, 1304
PstI	CTGCAG	2 cut(s)	1012, 1798
PsuI	RGATCY	1 cut(s)	664
PsyI	GACNNNGTC	1 cut(s)	1451
PvuII	CAGCTG	3 cut(s)	770, 1822, 1979
RsaI	GTAC	3 cut(s)	1125, 1626, 1809
RsaNI	GTAC	3 cut(s)	1124, 1625, 1808
RseI	CAYNNNNRTG	2 cut(s)	1668, 2208
SapI	GCTCTTC	1 cut(s)	570
SaqAI	TTAA	6 cut(s)	150, 171, 542, 710, 732, 1604
SatI	GCNGC	4 cut(s)	768, 833, 967, 1578
Sau96I	GGNCC	2 cut(s)	934, 1304
SchI	GAGTC	4 cut(s)	1298, 1787, 2042, 2064
ScrFI	CCNGG	3 cut(s)	932, 1533, 2076
SduI	GDGCHC	1 cut(s)	2193
SfcI	CTRYAG	3 cut(s)	701, 1008, 1794
SfoI	GGCGCC	1 cut(s)	1857
SinI	GGWCC	1 cut(s)	1304
SmiMI	CAYNNNNRTG	2 cut(s)	1668, 2208
SmlI	CTYRAG	3 cut(s)	224, 577, 1775
SmoI	CTYRAG	3 cut(s)	224, 577, 1775
SnaBI	TACGTA	1 cut(s)	846
SphI	GCATGC	1 cut(s)	1667
SsiI	CCGC	2 cut(s)	791, 832
SspDI	GGCGCC	1 cut(s)	1855
SspI	AATATT	1 cut(s)	439
SspMI	CTAG	7 cut(s)	414, 431, 495, 1353, 1493, 1581, 2049
StyD4I	CCNGG	3 cut(s)	930, 1531, 2074
StyI	CCWWGG	1 cut(s)	937
TaaI	ACNGT	5 cut(s)	887, 1246, 2082, 2195, 2221
TaiI	ACGT	2 cut(s)	289, 848
TaqI	TCGA	4 cut(s)	155, 1803, 1878, 2165
TatI	WGTACW	2 cut(s)	1123, 1807
TauI	GCSGC	1 cut(s)	835
TfiI	GAWTC	6 cut(s)	365, 659, 717, 1004, 1528, 1546
Tru1I	TTAA	6 cut(s)	150, 171, 542, 710, 732, 1604
Tru9I	TTAA	6 cut(s)	150, 171, 542, 710, 732, 1604
TscAI	CASTG	9 cut(s)	282, 805, 1017, 1214, 1344, 1349, 1675, 1993, 2200
TseFI	GTSAC	3 cut(s)	793, 1155, 1445
TseI	GCWGC	3 cut(s)	767, 966, 1577
Tsp45I	GTSAC	3 cut(s)	793, 1155, 1445
TspGWI	ACGGA	1 cut(s)	1721
TspRI	CASTG	9 cut(s)	282, 805, 1017, 1214, 1344, 1349, 1675, 1993, 2200
Tth111I	GACNNNGTC	1 cut(s)	1451
VneI	GTGCAC	1 cut(s)	2189
VpaK11BI	GGWCC	1 cut(s)	1304
VspI	ATTAAT	1 cut(s)	732
XapI	RAATTY	4 cut(s)	631, 926, 1078, 1674
XbaI	TCTAGA	1 cut(s)	1492
XceI	RCATGY	1 cut(s)	1667
XmnI	GAANNNNTTC	1 cut(s)	369
XspI	CTAG	7 cut(s)	414, 431, 495, 1353, 1493, 1581, 2049
Zsp2I	ATGCAT	4 cut(s)	33, 1589, 1760, 2005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.