Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Forward (+)

3549388 .. 3554408

Gene Structure 5021 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ42011

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGGACAACATAGCCTCTGATTTAGACCTCGCAGAGACGAAGCAGAGCAATCTGAGCAGAGCCTATGAGAGCCTACACACCCAAGCTTCTCCCTTTATGGTCCTAGGTCCTCAGTGGAAGGACCTCGAGGACCACCTCGACTCGACCCGGACCAAAATCCAGACCCGGTTCCAGGAGCTGCAGGCCCGGGAAGCCCAAATCGCCGACCAGGTGAAAAAGCTTGAGTCTTTGAAGGAGGTTGAGTACGGTAAGAGTCACTTGCAGTCTTTAAAGTTGTTAATTGAAGAAACTAGTGAAGAGCTTGGTGTGAAAGAGAAGAGGTTTTCGGAAGTTGAGAGGTTGATTAGAGTGAAGGAGAGAATGCTTGAGGATAGGAATAAGATGTTGAGTTTGGTTGAGAGGAGGATAGAGGAGAAGGGTAGGGAGGTTGAGGGGAAGGAGAAGAAGGTGAGGGTGGTGCAAGGAGTGTTGGATAAGTACTGTGTTGATATTAGGAAGAGGAAGAAGGAGTGTGGTCTGAGAGGAAAAGAGGTTAAGAGGGTGGAGAGGTTGATTGAGAAGTGTGACAAGGAGTTGAGTTTGAAGTGGGGGGAAATGGAAGGGATTCAGAAATCGATGGTGGCGTATTCGGATAGGATTAAGTCGAAAGAGGGGGTTATTAGGGAGATGGAGTTGAAGGTGAAGGAGTTTGGGATGCATAAGAAGGCAATGGAGGAGTGGTGTTGCAAGGTTGAAGTGAAGAAGAGGGAGCTTGAAGTGTGGGTGGAGAAGGTTGAGCCGAGGGGGAGAGAGTTTGAGCCGAGAGTTGAGGAACTTGATTTGATTGGTAAGAGGGTTAATGAGTGTCTCAATGAGGCTCAGTTGACATTGGAAAGGGATTTCCGTTTGCTTGAGGAAATGAGACAGGAGAATGTGAAGCATTTTGAGTTAGTTGAGAAGTCAGTGCAAGAACGTTCTCATGAACTTGAAATGAAAGAGAGGAGACTTGAAGAAAAGGCAAAAGAGCTTGACTTGAAACAAAAACTATTGGAATCTATTCCGAAAGCTACTGGAGAACAAATGAAATCGAAGGAGAAGGCTAGTATTGTTCATCCTTTAGTGAATATTCCACATATGTTCCTTGCAAACAATGTCGTCGTTCCTTCAACAGCAAGTAATGCTAGAGGTTTGCAGTTGATCGTGAGTGAGCATTTGAAGAGAATTGATTTAATGAGTAGAGAAATATCAGCTCTTCTTCAAGCCTCAAGAGACCCAGCAGGATTGGTTTTGGATGCAATGCAGGGGTTTTACCCTACAAATTCAACGGTGGACAACAGGGAGCTTGATTCGGATCTAAGAGTGATTAGAAGGAGTTGTATTGTATTGCTACAGGAGTTAAAGAGATTCTCGCCACAAATTAATGCTCAGGTCAGAGAAAAGGCAATGAAGTTGGCAGCTGAGTGGAAGGCTAAGTTGTCGGTGACCACTGAGAATAGGCTGGAGGTGTTGGGCTTCTTGCGGCTTCTTACTACGTATGAATTGACCTCCATGTATGATCCAAAGGAGCTTCACAGTCTTCTCTCTATAGTTGTTCAGCCTGAACAAACAACTGAATTCCAGGCCCTTGGTGCCTCAGATAAGGCATTTGTGAGCAGCACAATTTCTTTCCCAGTTAGAATTGAGGAACCGGAATCTTCAGTGGCCAAATTTGTAGCCCCCTTTTCAGCTCCAAATCTTCAACTAAGTGCAACCAGAGAACCAACAAATTTTCAGGGGTTCATAGTTAAGCGTTTGAGTGAGAATAATTCAGTACAGGACAAAATGTTGGCCACTCTTCAAATGTCACCAGACCCAGCACAAATTGTGTTGGAAATGCTGCAAAGCTCTTTTGCTCAGTGTTGGAGAGAAGGAGGTTTTTTTTCAGAAGTAACTGTCATGAAGGGTTACATTTACCTACTGGAGACACTAATGAGAGAGTCAAAGCACATTGGACCTCGTGTGAAAGAAGATGCAAGAAAGCTGGCAGTGCAGTGGAAAACTAGAATGAGAGCCGATGCTGGCAACTCGTTGGAGATTTTACTATTTTTGCAGTTTTTGGCTACATATGAATTGCTCTCTACCATAAATGGAGGTGACATTGTCAATCTTCTTGGGGTGATTTCTCGGCATAGACAGGCTCTAGAACTATGTCAGTCAGTTGGCTTTGCAGATAAGATTCCTGGTTTTATTTGGAATCTCATTGACAGGAAGCAACTAATTGATGCTGTTAGATGCATTTGCATCTTCAAGTTAATAGACAAGTTCCCTGCTGTACAACTTTTGAAAGATCATGTGCATAATGCAAGAAAGCATGCCAGTGAAATTTGCAAAAATCAGTTATCATTTGGTGAAACGGAGAAGGTTGTAGATGGTCTAATAGGTGATCTAAGAGCTGTGCATCAATGCATCAAAGACTACAATCTTGAGTCCGAATATCCATCTGCAGACATCGAAGTACAAGTAATTCAGCTGGGAAGACTGAAGGAGCATTACAGAAGTTTGGCGCCATCTCTTGCCTTCAGGGTCGATCAACAAGATCAGAGAAAAAGGAAGAGACCAAGCACCAGCACTTCTGCCCCCATCACTCAACCACATGAACAGCTTGAACAAAGTAATCACCAGACAGCTGTATCCACTGCTATACCCTATGCACTGCCGATTTCCACTTCAATTTGTCCAGAGTCAAGTTCGTTATCTAGGCTGTATGCAAATTATGGACTCCCTGGACAGTTTGGTATGGCAGCCAATGACCACGCAACCTGTGCTAATTCTGAAGGTGGCTTGATGCTAACATTGACAGGCCAATATGGATCGAATGTCAGAAATCCACTGCAAGTGCACAGTGCTGATCATGACCATGCTCATACAGTCATCCTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

966

Amino Acids

110.32

Weight (kDa)

7.12

Isoelectric Point (pI)

44.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	386 - 531	3.2e-29	Frigida-like protein
Frigida	PF07899	581 - 836	5.1e-57	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	125
AccB1I	GGYRCC	2 cut(s)	1607, 2521
AciI	CCGC	1 cut(s)	1498
AclI	AACGTT	1 cut(s)	952
AclWI	GGATC	3 cut(s)	1338, 1529, 2836
AcoI	YGGCCR	2 cut(s)	1680, 1806
AcsI	RAATTY	5 cut(s)	1297, 1592, 1685, 1744, 2340
AcuI	CTGAAG	4 cut(s)	1659, 2519, 2521, 2811
AcyI	GRCGYC	1 cut(s)	2522
AfaI	GTAC	5 cut(s)	245, 479, 1791, 2292, 2475
AfiI	CCNNNNNNNGG	1 cut(s)	172
AhlI	ACTAGT	1 cut(s)	290
AjnI	CCWGG	5 cut(s)	171, 207, 1596, 2197, 2740
Alw21I	GWGCWC	1 cut(s)	2859
Alw26I	GTCTC	7 cut(s)	29, 851, 895, 976, 1242, 1934, 2566
Alw44I	GTGCAC	1 cut(s)	2855
AlwI	GGATC	3 cut(s)	1338, 1529, 2836
AlwNI	CAGNNNCTG	1 cut(s)	178
Ama87I	CYCGRG	2 cut(s)	125, 186
AoxI	GGCC	5 cut(s)	183, 1599, 1680, 1806, 2818
ApaLI	GTGCAC	1 cut(s)	2855
ApeKI	GCWGC	5 cut(s)	178, 1433, 1632, 1855, 2759
ApoI	RAATTY	5 cut(s)	1297, 1592, 1685, 1744, 2340
AseI	ATTAAT	1 cut(s)	1398
Asp700I	GAANNNNTTC	2 cut(s)	603, 1035
AspA2I	CCTAGG	1 cut(s)	103
AspLEI	GCGC	1 cut(s)	2524
AspS9I	GGNCC	8 cut(s)	100, 107, 121, 130, 150, 184, 1600, 1970
AsuC2I	CCSGG	4 cut(s)	148, 166, 187, 188
AvaI	CYCGRG	2 cut(s)	125, 186
AvaII	GGWCC	6 cut(s)	100, 107, 121, 130, 150, 1970
AvrII	CCTAGG	1 cut(s)	103
BaeGI	GKGCMC	1 cut(s)	2859
BaeI	ACNNNNGTAYC	2 cut(s)	2631, 2664
BalI	TGGCCA	2 cut(s)	1682, 1808
BanI	GGYRCC	2 cut(s)	1607, 2521
BarI	GAAGNNNNNNTAC	2 cut(s)	2492, 2524
BauI	CACGAG	1 cut(s)	1974
BbsI	GAAGAC	2 cut(s)	1547, 2500
Bbv12I	GWGCWC	1 cut(s)	2859
BbvI	GCAGC	5 cut(s)	165, 1445, 1644, 1842, 2771
BccI	CCATC	6 cut(s)	610, 661, 2381, 2464, 2533, 2606
BciT130I	CCWGG	5 cut(s)	173, 209, 1598, 2199, 2742
BciVI	GTATCC	1 cut(s)	2659
BclI	TGATCA	1 cut(s)	2866
BcnI	CCSGG	4 cut(s)	148, 166, 187, 188
BcoDI	GTCTC	7 cut(s)	29, 851, 895, 976, 1242, 1934, 2566
BcuI	ACTAGT	1 cut(s)	290
BfaI	CTAG	7 cut(s)	104, 291, 1080, 1161, 2019, 2159, 2715
BfmI	CTRYAG	4 cut(s)	179, 1367, 1563, 2460
BfoI	RGCGCY	1 cut(s)	2525
BfuI	GTATCC	1 cut(s)	2659
BisI	GCNGC	6 cut(s)	179, 1434, 1499, 1633, 1856, 2760
BlnI	CCTAGG	1 cut(s)	103
BlsI	GCNGC	6 cut(s)	180, 1435, 1500, 1634, 1857, 2761
BmcAI	AGTACT	1 cut(s)	479
Bme1390I	CCNGG	9 cut(s)	148, 166, 173, 187, 188, 209, 1598, 2199, 2742
Bme18I	GGWCC	6 cut(s)	100, 107, 121, 130, 150, 1970
BmeT110I	CYCGRG	2 cut(s)	125, 186
BmgT120I	GGNCC	8 cut(s)	100, 107, 121, 130, 150, 184, 1600, 1970
BmiI	GGNNCC	4 cut(s)	170, 1609, 1665, 2523
BmrFI	CCNGG	9 cut(s)	148, 166, 173, 187, 188, 209, 1598, 2199, 2742
BmrI	ACTGGG	1 cut(s)	1643
BmuI	ACTGGG	1 cut(s)	1643
BpiI	GAAGAC	2 cut(s)	1547, 2500
BpmI	CTGGAG	3 cut(s)	1071, 1499, 1958
Bpu10I	CCTNAGC	1 cut(s)	1404
BpuEI	CTTGAG	5 cut(s)	242, 386, 911, 1228, 2462
BpuMI	CCSGG	4 cut(s)	148, 166, 187, 188
Bsa29I	ATCGAT	1 cut(s)	614
BsaAI	YACGTR	1 cut(s)	1512
BsaBI	GATNNNNATC	1 cut(s)	1329
BsaHI	GRCGYC	1 cut(s)	2522
BsaI	GGTCTC	2 cut(s)	1242, 2566
BsaJI	CCNNGG	5 cut(s)	103, 186, 779, 1603, 2740
BsaWI	WCCGGW	1 cut(s)	1666
BsaXI	ACNNNNNCTCC	6 cut(s)	227, 257, 656, 686, 704, 734
Bsc4I	CCNNNNNNNGG	1 cut(s)	172
Bse1I	ACTGG	4 cut(s)	1054, 1649, 1941, 2334
Bse3DI	GCAATG	3 cut(s)	714, 1281, 1428
Bse8I	GATNNNNATC	1 cut(s)	1329
BseBI	CCWGG	5 cut(s)	173, 209, 1598, 2199, 2742
BseCI	ATCGAT	1 cut(s)	614
BseDI	CCNNGG	5 cut(s)	103, 186, 779, 1603, 2740
BseGI	GGATG	4 cut(s)	699, 1090, 1276, 2889
BseJI	GATNNNNATC	1 cut(s)	1329
BseLI	CCNNNNNNNGG	1 cut(s)	172
BseMI	GCAATG	3 cut(s)	714, 1281, 1428
BseMII	CTCAG	9 cut(s)	44, 125, 509, 872, 1418, 1428, 1458, 1626, 1886
BseNI	ACTGG	4 cut(s)	1054, 1649, 1941, 2334
BseRI	GAGGAG	4 cut(s)	415, 425, 728, 994
BseSI	GKGCMC	1 cut(s)	2859
BseXI	GCAGC	5 cut(s)	165, 1445, 1644, 1842, 2771
BseYI	CCCAGC	3 cut(s)	1252, 1831, 2488
BsgI	GTGCAG	1 cut(s)	2027
BshFI	GGCC	5 cut(s)	185, 1601, 1682, 1808, 2820
BshNI	GGYRCC	2 cut(s)	1607, 2521
BshVI	ATCGAT	1 cut(s)	614
BsiHKAI	GWGCWC	1 cut(s)	2859
BsiHKCI	CYCGRG	2 cut(s)	125, 186
BsiSI	CCGG	4 cut(s)	148, 166, 187, 1667
BslI	CCNNNNNNNGG	1 cut(s)	172
BsmAI	GTCTC	7 cut(s)	29, 851, 895, 976, 1242, 1934, 2566
BsmBI	CGTCTC	1 cut(s)	29
BsmI	GAATGC	1 cut(s)	366
BsnI	GGCC	5 cut(s)	185, 1601, 1682, 1808, 2820
Bso31I	GGTCTC	2 cut(s)	1242, 2566
BsoBI	CYCGRG	2 cut(s)	125, 186
Bsp1286I	GDGCHC	1 cut(s)	2859
Bsp1407I	TGTACA	1 cut(s)	2290
Bsp143I	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
BspACI	CCGC	1 cut(s)	1498
BspANI	GGCC	5 cut(s)	185, 1601, 1682, 1808, 2820
BspCNI	CTCAG	9 cut(s)	45, 124, 510, 871, 1417, 1429, 1459, 1625, 1885
BspDI	ATCGAT	1 cut(s)	614
BspHI	TCATGA	3 cut(s)	958, 1914, 2869
BspLI	GGNNCC	4 cut(s)	170, 1609, 1665, 2523
BspMAI	CTGCAG	2 cut(s)	183, 2464
BspPI	GGATC	3 cut(s)	1338, 1529, 2836
BspQI	GCTCTTC	2 cut(s)	291, 1236
BspT107I	GGYRCC	2 cut(s)	1607, 2521
BspTNI	GGTCTC	2 cut(s)	1242, 2566
BsrDI	GCAATG	3 cut(s)	714, 1281, 1428
BsrGI	TGTACA	1 cut(s)	2290
BsrI	ACTGG	4 cut(s)	1054, 1649, 1941, 2334
BssECI	CCNNGG	5 cut(s)	103, 186, 779, 1603, 2740
BssMI	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
BssNI	GRCGYC	1 cut(s)	2522
BssSI	CACGAG	1 cut(s)	1974
BssT1I	CCWWGG	2 cut(s)	103, 1603
Bst2BI	CACGAG	1 cut(s)	1974
Bst2UI	CCWGG	5 cut(s)	173, 209, 1598, 2199, 2742
Bst4CI	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2748, 2861, 2887
Bst6I	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2564
BstACI	GRCGYC	1 cut(s)	2522
BstAPI	GCANNNNNTGC	2 cut(s)	1157, 2780
BstAUI	TGTACA	1 cut(s)	2290
BstBAI	YACGTR	1 cut(s)	1512
BstC8I	GCNNGC	4 cut(s)	183, 2001, 2038, 2331
BstEII	GGTNACC	1 cut(s)	1459
BstF5I	GGATG	4 cut(s)	699, 1090, 1276, 2889
BstH2I	RGCGCY	1 cut(s)	2525
BstHHI	GCGC	1 cut(s)	2524
BstKTI	GATC	9 cut(s)	1179, 1333, 1537, 2308, 2404, 2548, 2557, 2831, 2869
BstMAI	GTCTC	7 cut(s)	29, 851, 895, 976, 1242, 1934, 2566
BstMBI	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
BstMWI	GCNNNNNNNGC	7 cut(s)	54, 191, 200, 1157, 1607, 2005, 2780
BstNI	CCWGG	5 cut(s)	173, 209, 1598, 2199, 2742
BstNSI	RCATGY	1 cut(s)	2333
BstPI	GGTNACC	1 cut(s)	1459
BstSCI	CCNGG	9 cut(s)	146, 164, 171, 185, 186, 207, 1596, 2197, 2740
BstSFI	CTRYAG	4 cut(s)	179, 1367, 1563, 2460
BstSLI	GKGCMC	1 cut(s)	2859
BstSNI	TACGTA	1 cut(s)	1512
BstV1I	GCAGC	5 cut(s)	165, 1445, 1644, 1842, 2771
BstV2I	GAAGAC	2 cut(s)	1547, 2500
BstX2I	RGATCY	1 cut(s)	1330
BstXI	CCANNNNNNTGG	1 cut(s)	1604
BstYI	RGATCY	1 cut(s)	1330
Bsu15I	ATCGAT	1 cut(s)	614
BsuI	GTATCC	1 cut(s)	2659
BsuRI	GGCC	5 cut(s)	185, 1601, 1682, 1808, 2820
BsuTUI	ATCGAT	1 cut(s)	614
BtsCI	GGATG	4 cut(s)	699, 1090, 1276, 2889
BtsI	GCAGTG	5 cut(s)	2010, 2015, 2652, 2669, 2846
Cac8I	GCNNGC	4 cut(s)	183, 2001, 2038, 2331
CaiI	CAGNNNCTG	1 cut(s)	178
CciI	TCATGA	3 cut(s)	958, 1914, 2869
CfoI	GCGC	1 cut(s)	2524
Cfr13I	GGNCC	8 cut(s)	100, 107, 121, 130, 150, 184, 1600, 1970
Cfr9I	CCCGGG	1 cut(s)	186
ClaI	ATCGAT	1 cut(s)	614
CsiI	ACCWGGT	1 cut(s)	207
Csp6I	GTAC	5 cut(s)	244, 478, 1790, 2291, 2474
CviAII	CATG	8 cut(s)	959, 1528, 1915, 2309, 2330, 2612, 2870, 2876
CviQI	GTAC	5 cut(s)	244, 478, 1790, 2291, 2474
DinI	GGCGCC	1 cut(s)	2523
DpnI	GATC	9 cut(s)	1178, 1332, 1536, 2307, 2403, 2547, 2556, 2830, 2868
DpnII	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
DraI	TTTAAA	1 cut(s)	270
EaeI	YGGCCR	2 cut(s)	1680, 1806
Eam1104I	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2564
EarI	CTCTTC	8 cut(s)	291, 311, 491, 737, 1190, 1236, 1818, 2564
Eco105I	TACGTA	1 cut(s)	1512
Eco130I	CCWWGG	2 cut(s)	103, 1603
Eco31I	GGTCTC	2 cut(s)	1242, 2566
Eco47I	GGWCC	6 cut(s)	100, 107, 121, 130, 150, 1970
Eco57I	CTGAAG	4 cut(s)	1659, 2519, 2521, 2811
Eco88I	CYCGRG	2 cut(s)	125, 186
Eco91I	GGTNACC	1 cut(s)	1459
EcoO109I	RGGNCCY	3 cut(s)	107, 121, 1600
EcoO65I	GGTNACC	1 cut(s)	1459
EcoRI	GAATTC	1 cut(s)	1592
EcoRII	CCWGG	5 cut(s)	171, 207, 1596, 2197, 2740
EcoT14I	CCWWGG	2 cut(s)	103, 1603
EcoT22I	ATGCAT	3 cut(s)	699, 2255, 2426
EgeI	GGCGCC	1 cut(s)	2523
EheI	GGCGCC	1 cut(s)	2523
ErhI	CCWWGG	2 cut(s)	103, 1603
Esp3I	CGTCTC	1 cut(s)	29
FaeI	CATG	8 cut(s)	962, 1531, 1918, 2312, 2333, 2615, 2873, 2879
FalI	AAGNNNNNCTT	2 cut(s)	242, 274
FatI	CATG	8 cut(s)	958, 1527, 1914, 2308, 2329, 2611, 2869, 2875
FauNDI	CATATG	2 cut(s)	1113, 2083
FbaI	TGATCA	1 cut(s)	2866
Fnu4HI	GCNGC	6 cut(s)	179, 1434, 1499, 1633, 1856, 2760
FokI	GGATG	4 cut(s)	706, 1077, 1283, 2876
Fsp4HI	GCNGC	6 cut(s)	179, 1434, 1499, 1633, 1856, 2760
FspBI	CTAG	7 cut(s)	104, 291, 1080, 1161, 2019, 2159, 2715
GlaI	GCGC	1 cut(s)	2523
GluI	GCNGC	6 cut(s)	179, 1434, 1499, 1633, 1856, 2760
GsaI	CCCAGC	3 cut(s)	1256, 1835, 2492
GsuI	CTGGAG	3 cut(s)	1071, 1499, 1958
HaeII	RGCGCY	1 cut(s)	2525
HaeIII	GGCC	5 cut(s)	185, 1601, 1682, 1808, 2820
HapII	CCGG	4 cut(s)	148, 166, 187, 1667
HhaI	GCGC	1 cut(s)	2524
Hin1I	GRCGYC	1 cut(s)	2522
Hin1II	CATG	8 cut(s)	962, 1531, 1918, 2312, 2333, 2615, 2873, 2879
Hin6I	GCGC	1 cut(s)	2522
HinP1I	GCGC	1 cut(s)	2522
HincII	GTYRAC	1 cut(s)	864
HindII	GTYRAC	1 cut(s)	864
HindIII	AAGCTT	2 cut(s)	84, 218
HpaII	CCGG	4 cut(s)	148, 166, 187, 1667
Hpy166II	GTNNAC	3 cut(s)	864, 1309, 2857
Hpy188III	TCNNGA	9 cut(s)	160, 959, 1180, 1245, 1915, 2159, 2441, 2696, 2870
Hpy8I	GTNNAC	3 cut(s)	864, 1309, 2857
Hpy99I	CGWCG	1 cut(s)	1139
HpyCH4III	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2748, 2861, 2887
HpyCH4IV	ACGT	2 cut(s)	952, 1511
HpyF10VI	GCNNNNNNNGC	7 cut(s)	54, 191, 200, 1157, 1607, 2005, 2780
HpySE526I	ACGT	2 cut(s)	952, 1511
Hsp92I	GRCGYC	1 cut(s)	2522
Hsp92II	CATG	8 cut(s)	962, 1531, 1918, 2312, 2333, 2615, 2873, 2879
HspAI	GCGC	1 cut(s)	2522
KasI	GGCGCC	1 cut(s)	2521
Ksp22I	TGATCA	1 cut(s)	2866
Kzo9I	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
LguI	GCTCTTC	2 cut(s)	291, 1236
LmnI	GCTCC	6 cut(s)	175, 748, 1318, 1543, 1711, 2503
Lsp1109I	GCAGC	5 cut(s)	165, 1445, 1644, 1842, 2771
MabI	ACCWGGT	1 cut(s)	207
MaeI	CTAG	7 cut(s)	104, 291, 1080, 1161, 2019, 2159, 2715
MaeII	ACGT	2 cut(s)	952, 1511
MaeIII	GTNAC	7 cut(s)	254, 563, 1459, 1821, 1906, 1922, 2111
MalI	GATC	9 cut(s)	1178, 1332, 1536, 2307, 2403, 2547, 2556, 2830, 2868
MboI	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	1 cut(s)	2859
MlsI	TGGCCA	2 cut(s)	1682, 1808
MluNI	TGGCCA	2 cut(s)	1682, 1808
Mly113I	GGCGCC	1 cut(s)	2522
MlyI	GAGTC	7 cut(s)	134, 233, 262, 1964, 2453, 2708, 2730
MmeI	TCCRAC	4 cut(s)	450, 1827, 1859, 2028
Mox20I	TGGCCA	2 cut(s)	1682, 1808
Mph1103I	ATGCAT	3 cut(s)	699, 2255, 2426
MroXI	GAANNNNTTC	2 cut(s)	603, 1035
MscI	TGGCCA	2 cut(s)	1682, 1808
MslI	CAYNNNNRTG	2 cut(s)	2334, 2874
Msp20I	TGGCCA	2 cut(s)	1682, 1808
MspA1I	CMGCKG	3 cut(s)	1436, 2488, 2645
MspI	CCGG	4 cut(s)	148, 166, 187, 1667
MspR9I	CCNGG	9 cut(s)	148, 166, 173, 187, 188, 209, 1598, 2199, 2742
Mva1269I	GAATGC	1 cut(s)	366
MvaI	CCWGG	5 cut(s)	173, 209, 1598, 2199, 2742
MwoI	GCNNNNNNNGC	7 cut(s)	54, 191, 200, 1157, 1607, 2005, 2780
NarI	GGCGCC	1 cut(s)	2522
NciI	CCSGG	4 cut(s)	148, 166, 187, 188
NdeI	CATATG	2 cut(s)	1113, 2083
NdeII	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
NlaIII	CATG	8 cut(s)	962, 1531, 1918, 2312, 2333, 2615, 2873, 2879
NlaIV	GGNNCC	4 cut(s)	170, 1609, 1665, 2523
NmeAIII	GCCGAG	3 cut(s)	804, 825, 2122
NmuCI	GTSAC	5 cut(s)	254, 563, 1459, 1821, 2111
NsiI	ATGCAT	3 cut(s)	699, 2255, 2426
NspI	RCATGY	1 cut(s)	2333
PaeI	GCATGC	1 cut(s)	2333
PaeR7I	CTCGAG	1 cut(s)	125
PagI	TCATGA	3 cut(s)	958, 1914, 2869
PciSI	GCTCTTC	2 cut(s)	291, 1236
PcsI	WCGNNNNNNNCGW	1 cut(s)	620
PctI	GAATGC	1 cut(s)	366
PdmI	GAANNNNTTC	2 cut(s)	603, 1035
PfeI	GAWTC	7 cut(s)	604, 1031, 1325, 1383, 1670, 2194, 2212
PflFI	GACNNNGTC	1 cut(s)	2117
PfoI	TCCNGGA	1 cut(s)	171
PkrI	GCNGC	6 cut(s)	180, 1435, 1500, 1634, 1857, 2761
PleI	GAGTC	7 cut(s)	134, 232, 261, 1963, 2452, 2707, 2730
PluTI	GGCGCC	1 cut(s)	2525
PpsI	GAGTC	7 cut(s)	134, 232, 261, 1963, 2452, 2707, 2730
Ppu21I	YACGTR	1 cut(s)	1512
PpuMI	RGGWCCY	2 cut(s)	107, 121
PshBI	ATTAAT	1 cut(s)	1398
Psp1406I	AACGTT	1 cut(s)	952
Psp5II	RGGWCCY	2 cut(s)	107, 121
Psp6I	CCWGG	5 cut(s)	171, 207, 1596, 2197, 2740
PspEI	GGTNACC	1 cut(s)	1459
PspFI	CCCAGC	3 cut(s)	1252, 1831, 2488
PspGI	CCWGG	5 cut(s)	171, 207, 1596, 2197, 2740
PspN4I	GGNNCC	4 cut(s)	170, 1609, 1665, 2523
PspPI	GGNCC	8 cut(s)	100, 107, 121, 130, 150, 184, 1600, 1970
PspPPI	RGGWCCY	2 cut(s)	107, 121
PspXI	VCTCGAGB	1 cut(s)	125
PstI	CTGCAG	2 cut(s)	183, 2464
PstNI	CAGNNNCTG	1 cut(s)	178
PsuI	RGATCY	1 cut(s)	1330
PsyI	GACNNNGTC	1 cut(s)	2117
PvuII	CAGCTG	3 cut(s)	1436, 2488, 2645
RsaI	GTAC	5 cut(s)	245, 479, 1791, 2292, 2475
RsaNI	GTAC	5 cut(s)	244, 478, 1790, 2291, 2474
RseI	CAYNNNNRTG	2 cut(s)	2334, 2874
SapI	GCTCTTC	2 cut(s)	291, 1236
SatI	GCNGC	6 cut(s)	179, 1434, 1499, 1633, 1856, 2760
Sau3AI	GATC	9 cut(s)	1176, 1330, 1534, 2305, 2401, 2545, 2554, 2828, 2866
Sau96I	GGNCC	8 cut(s)	100, 107, 121, 130, 150, 184, 1600, 1970
ScaI	AGTACT	1 cut(s)	479
SchI	GAGTC	7 cut(s)	134, 233, 262, 1964, 2453, 2708, 2730
ScrFI	CCNGG	9 cut(s)	148, 166, 173, 187, 188, 209, 1598, 2199, 2742
SduI	GDGCHC	1 cut(s)	2859
SexAI	ACCWGGT	1 cut(s)	207
SfcI	CTRYAG	4 cut(s)	179, 1367, 1563, 2460
SfoI	GGCGCC	1 cut(s)	2523
Sfr274I	CTCGAG	1 cut(s)	125
SinI	GGWCC	6 cut(s)	100, 107, 121, 130, 150, 1970
SlaI	CTCGAG	1 cut(s)	125
SmaI	CCCGGG	1 cut(s)	188
SmiMI	CAYNNNNRTG	2 cut(s)	2334, 2874
SmlI	CTYRAG	6 cut(s)	125, 221, 365, 890, 1243, 2441
SmoI	CTYRAG	6 cut(s)	125, 221, 365, 890, 1243, 2441
SnaBI	TACGTA	1 cut(s)	1512
SpeI	ACTAGT	1 cut(s)	290
SphI	GCATGC	1 cut(s)	2333
SsiI	CCGC	1 cut(s)	1498
SspDI	GGCGCC	1 cut(s)	2521
SspI	AATATT	1 cut(s)	1105
SspMI	CTAG	7 cut(s)	104, 291, 1080, 1161, 2019, 2159, 2715
StyD4I	CCNGG	9 cut(s)	146, 164, 171, 185, 186, 207, 1596, 2197, 2740
StyI	CCWWGG	2 cut(s)	103, 1603
TaaI	ACNGT	8 cut(s)	248, 482, 1306, 1553, 1912, 2748, 2861, 2887
TaiI	ACGT	2 cut(s)	955, 1514
TaqI	TCGA	9 cut(s)	126, 138, 143, 614, 644, 1067, 2469, 2544, 2831
TatI	WGTACW	4 cut(s)	477, 1789, 2290, 2473
TauI	GCSGC	1 cut(s)	1501
TfiI	GAWTC	7 cut(s)	604, 1031, 1325, 1383, 1670, 2194, 2212
TseFI	GTSAC	5 cut(s)	254, 563, 1459, 1821, 2111
TseI	GCWGC	5 cut(s)	178, 1433, 1632, 1855, 2759
Tsp45I	GTSAC	5 cut(s)	254, 563, 1459, 1821, 2111
TspGWI	ACGGA	2 cut(s)	872, 2387
TspMI	CCCGGG	1 cut(s)	186
Tth111I	GACNNNGTC	1 cut(s)	2117
VneI	GTGCAC	1 cut(s)	2855
VpaK11BI	GGWCC	6 cut(s)	100, 107, 121, 130, 150, 1970
VspI	ATTAAT	1 cut(s)	1398
XapI	RAATTY	5 cut(s)	1297, 1592, 1685, 1744, 2340
XbaI	TCTAGA	1 cut(s)	2158
XceI	RCATGY	1 cut(s)	2333
XhoI	CTCGAG	1 cut(s)	125
XmaI	CCCGGG	1 cut(s)	186
XmaJI	CCTAGG	1 cut(s)	103
XmnI	GAANNNNTTC	2 cut(s)	603, 1035
XspI	CTAG	7 cut(s)	104, 291, 1080, 1161, 2019, 2159, 2715
ZrmI	AGTACT	1 cut(s)	479
Zsp2I	ATGCAT	3 cut(s)	699, 2255, 2426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.