Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

20776518 .. 20780145

Gene Structure 3628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g18950.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGGAAACGATGGTGTCCGGTTCGGATGAACCGGAGTTGAAGCAAAGGAATTTGGTCCCGCAGTGGAAGCACCTCTTGGACCACATTGACTCGCTCCAGACCCGGTTCGAGGAGCTCCAGGACCGGTTTTACTCGGAGCAGAACTCGCTCCAGACACGGCAGCGGGAGGTTGAGGAGCGCGAGAAGGAGTTGGAGGGCAAAGTTTTGAACTTTAACTCGGAAATGGCGTCTAAAGCTCAGGAATTGTATGAAATCGAGAGATTGGCGAAAGAGAAAAGAAGGGAGGTTGGTGGTAAGGAAAAATGGTTGTTGGAAATCGAGGAATCGGTTAGGGGGAAGGCGAGGGAACTTGATTTGATAAAAAATGATATCGAAGAGCGAACGGAGAAATTGAATTCGATTGTGAAAAGTTTGGATGAGAAGTCGAGAGAGCTGAAGATGAAGAAGGAGGTCACTGAGTTGAACGGAAGGCGATTAAACTCGATGAAGGGGTCTATTGATGATGTCATGAAAGATTTTGTTTCGAAACAAGAGCAGGTTAAGGCGGCGCATAAATCTCTCGAAGAATGTCACAAGGAGATTCAATCGAAAAGGAAGATACTTGGTGCCGTTGAAAAATCGATAAGGGAATGTTACCGTACACTTGAATCCAAAGAGAAAACCATCAGGGCAGTGGAGTTGAAACAACAAGAATTTGAGTTGATGGTTCAAGAGTGCCAAAAGCATTTGGATTCACAAGAAAAGTTGTTGCAACAAGGTTGCCATGGACTCGAAATGAAAGAGAGGCAACTCGAAGAGCAGGTCAGAGTGTTTGAATCTGAACAACGACAATTTGATTTGACGGCTAAAGAACGCCAAAAATGTTTGGATTTGCAAGAAAAGTTGTTACAAGAAGGTTGCCATGGACTTGAAAAGAAAGAAGGGCGACTTGAGGAAAAGGTCAGAGAGTTTGAATCGAATAAAAAAGAATTTGATTCGATGATTCAAGAACGCCAACAACATTTGGATTCTCAAGAGAAGTTGTTACAAGAAGGTTCTCATGGACTTGAAATGAAAGAGAGGCAGCTTGAAGAACAGGTCAGAAAGCTCGAATCAGAAAAACAACAATTTGAGTCGCTGCAAAAATCCAGGGAAGACATCCAAAATCTGAAATCCAAGGAGAAGACTAATTGTTCCATGGATGGCAAATGCTTGCAGGTTCTCATGAATGAGAATTGGACGAGAACTGATCTTGTATGTGGCGAAATCGCAGCTTGTCTTCAGGCGTCATCTGACCCAGCAAAGCTCGTTTTGGATGCAATGCAAGGGTTTTATCCTTCAAATCAGACCGTGGACACCACTGAGTTTGCCTTTGATTTGACAGTTATTAGGAGGAGTTGTATTTTTTTGTTACAGCAGTTGAAGAGATTCTCACCACAAATCAACCCTCAGGTGAGAAAAGAAGCAAGGGCTTTGGCAGCTGAATGGAAGGGTAAGATGACTGTGGCGACTGAGAATGGATTGGAGATTTTGGGTTTTTTGGAGCTTGTTGCTGTGTATGAAATCACAACTGCTTATGATTCGAAGGAGCTTCAGAGTCTTCTTGGTACAGTGGCTGAGCACGAGCAAGGAACTGAACTATGCCAGGCCCTTGGTATCACAAGTAAGGCACCTGCGAGCAACAGCGTTTCTTTCCCTATCAAAATTGAGGAACAAGAATCTTCACAACTTATAAATGTCGCAGCTTCATCTTCTCTGGATCTTCAACCGAGTGCAACCACAGATACAATGAATTTGCAGGGCTCTACGGATGAGCATTTAAGTGAGTACGAGTCAGTGCAGAACGAAATGCTTCTTGATCTACAAATGTCAATGGACCCAGCAGAACTAGTTTTGAAGTTGATTAAAGAATCTTTGTCTCAATGCTGGAGAAAAAGAGATGTTGGCTGTGAATCAGCTGTCGTGGAGAATAGCATTTCCCTGTTGAATGATCTAACGGAAGTCTCCATGAAAATTGGGCCACATGTTAAAAAAAATGCAATAGAGCTAGCAGTACAGTGGAAAGCAAAAATGAGAGTTGATGCTGAAAATTCAATGGAGATTTTGGTGTTTTTGAACTTTGTAGCTACATATGGATTGGTTTCTACCTTTAACAGATATGAGATTGTAAAGCTTCTCGGGACGATTTGTCTGCACAAAAAGGCTTTGGAATCATGCCAAGCAATTGGTTTTGCAGATATGATCCCTGATTTTGTTCAAGATCTTATTGAAAGGAAGCAACTGATTGAAGCTGTTAGATTGATTTGCGGCTTCAACTTATCTGCCAGGTTCCCACCGGTACCACTCTTAAAAGAATATGTGGAAGGTGCAAGGCGGTCTTACTGCACAAAATGGCTGGTAAAAAGATCACTTGATGAAATGAATGAGGTGGTAGACAAGCAAATAGCTGATCTGAGCGCTGCGATTCATTGCATCAAAGACAATCATCTCGAGTCAGAATTCCCATACAAGGGCATCGAAAAAGAGATAGTTAAGCTGGAAAAGCACAAGTTGGATTGGAGACGTTCGGTTCCAGTTGTTGGCTCCAAGGGTGAAGGAGAGCAGGAGAAAGGGAAGAAACCAAATACCAACCTTGTATCCTTCAAGATCAAACCGCCAGAACAGCGTAACTGCAAGCGCCTCAAACAGCTGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

892

Amino Acids

102.57

Weight (kDa)

5.64

Isoelectric Point (pI)

56.52

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1712
AarI	CACCTGC	1 cut(s)	1660
Acc36I	ACCTGC	4 cut(s)	526, 790, 1186, 1660
Acc65I	GGTACC	1 cut(s)	2317
AccB1I	GGYRCC	3 cut(s)	605, 1648, 2317
AccB7I	CCANNNNNTGG	1 cut(s)	2558
AccI	GTMKAC	1 cut(s)	2412
AccII	CGCG	1 cut(s)	180
AciI	CCGC	6 cut(s)	59, 163, 545, 2286, 2353, 2633
AclWI	GGATC	2 cut(s)	1746, 2215
AcsI	RAATTY	7 cut(s)	49, 394, 692, 968, 1771, 2068, 2477
AcuI	CTGAAG	3 cut(s)	455, 1244, 1556
AcyI	GRCGYC	2 cut(s)	227, 1265
AfaI	GTAC	5 cut(s)	640, 1588, 1808, 2034, 2319
AfeI	AGCGCT	1 cut(s)	2437
AfiI	CCNNNNNNNGG	4 cut(s)	109, 2488, 2489, 2558
AflIII	ACRYGT	1 cut(s)	2002
AgeI	ACCGGT	2 cut(s)	123, 2314
AhdI	GACNNNNNGTC	1 cut(s)	2166
AhlI	ACTAGT	1 cut(s)	1867
AjnI	CCWGG	4 cut(s)	117, 1127, 1623, 2303
AjuI	GAANNNNNNNTTGG	2 cut(s)	59, 91
Alw21I	GWGCWC	2 cut(s)	117, 1602
Alw26I	GTCTC	3 cut(s)	1902, 1987, 2533
AlwI	GGATC	2 cut(s)	1746, 2215
AlwNI	CAGNNNCTG	1 cut(s)	1595
Ama87I	CYCGRG	2 cut(s)	2156, 2468
Aor51HI	AGCGCT	1 cut(s)	2437
AoxI	GGCC	3 cut(s)	1626, 1997, 2670
ApeKI	GCWGC	7 cut(s)	160, 1063, 1117, 1250, 1457, 1721, 2438
ApoI	RAATTY	7 cut(s)	49, 394, 692, 968, 1771, 2068, 2477
ArsI	GACNNNNNNTTYG	2 cut(s)	786, 818
AsiGI	ACCGGT	2 cut(s)	123, 2314
Asp700I	GAANNNNTTC	2 cut(s)	1406, 1830
Asp718I	GGTACC	1 cut(s)	2317
AspLEI	GCGC	4 cut(s)	180, 550, 2438, 2658
AspS9I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1855, 1997
AsuC2I	CCSGG	1 cut(s)	103
AsuHPI	GGTGA	3 cut(s)	1404, 1444, 2582
AsuII	TTCGAA	2 cut(s)	524, 1562
AsuNHI	GCTAGC	1 cut(s)	2026
AvaI	CYCGRG	2 cut(s)	2156, 2468
AvaII	GGWCC	4 cut(s)	55, 79, 121, 1855
AxyI	CCTNAGG	1 cut(s)	1428
BanI	GGYRCC	3 cut(s)	605, 1648, 2317
BanII	GRGCYC	2 cut(s)	117, 1784
BauI	CACGAG	1 cut(s)	1601
BbsI	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
Bbv12I	GWGCWC	2 cut(s)	117, 1602
BbvI	GCAGC	7 cut(s)	172, 1075, 1104, 1262, 1469, 1733, 2425
BccI	CCATC	4 cut(s)	4, 671, 697, 1175
BceAI	ACGGC	3 cut(s)	173, 593, 858
BcgI	CGANNNNNNTGC	2 cut(s)	2152, 2186
BciT130I	CCWGG	4 cut(s)	119, 1129, 1625, 2305
BciVI	GTATCC	1 cut(s)	2626
BcnI	CCSGG	1 cut(s)	103
BcoDI	GTCTC	3 cut(s)	1902, 1987, 2533
BcuI	ACTAGT	1 cut(s)	1867
BfaI	CTAG	2 cut(s)	1868, 2027
BfoI	RGCGCY	2 cut(s)	2439, 2659
BfuAI	ACCTGC	4 cut(s)	526, 790, 1186, 1660
BfuI	GTATCC	1 cut(s)	2626
BglII	AGATCT	1 cut(s)	2239
BisI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1722, 2287, 2439
BlpI	GCTNAGC	1 cut(s)	1596
BlsI	GCNGC	9 cut(s)	162, 547, 1065, 1119, 1252, 1459, 1723, 2288, 2440
Bme1390I	CCNGG	5 cut(s)	103, 119, 1129, 1625, 2305
Bme18I	GGWCC	4 cut(s)	55, 79, 121, 1855
BmeRI	GACNNNNNGTC	1 cut(s)	2166
BmeT110I	CYCGRG	2 cut(s)	2156, 2468
BmgT120I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1855, 1997
BmiI	GGNNCC	8 cut(s)	57, 607, 1650, 1857, 2309, 2319, 2550, 2563
BmrFI	CCNGG	5 cut(s)	103, 119, 1129, 1625, 2305
BmsI	GCATC	4 cut(s)	1285, 2050, 2460, 2502
BmtI	GCTAGC	1 cut(s)	2030
BpiI	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
BplI	GAGNNNNNCTC	2 cut(s)	128, 160
BpmI	CTGGAG	4 cut(s)	80, 101, 134, 1927
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu1102I	GCTNAGC	1 cut(s)	1596
Bpu14I	TTCGAA	2 cut(s)	524, 1562
BpuEI	CTTGAG	2 cut(s)	950, 996
BpuMI	CCSGG	1 cut(s)	103
Bsa29I	ATCGAT	1 cut(s)	620
BsaHI	GRCGYC	2 cut(s)	227, 1265
BsaJI	CCNNGG	8 cut(s)	763, 901, 1128, 1155, 1176, 1329, 1630, 2565
BsaWI	WCCGGW	4 cut(s)	17, 31, 123, 2314
BsaXI	ACNNNNNCTCC	2 cut(s)	1363, 1393
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 2488, 2489, 2558
Bse118I	RCCGGY	2 cut(s)	123, 2314
Bse1I	ACTGG	1 cut(s)	2552
Bse21I	CCTNAGG	1 cut(s)	1428
Bse3DI	GCAATG	2 cut(s)	1305, 2446
BseBI	CCWGG	4 cut(s)	119, 1129, 1625, 2305
BseCI	ATCGAT	1 cut(s)	620
BseDI	CCNNGG	8 cut(s)	763, 901, 1128, 1155, 1176, 1329, 1630, 2565
BseGI	GGATG	6 cut(s)	31, 421, 1137, 1186, 1300, 1795
BseLI	CCNNNNNNNGG	4 cut(s)	109, 2488, 2489, 2558
BseMI	GCAATG	2 cut(s)	1305, 2446
BseMII	CTCAG	7 cut(s)	251, 447, 1332, 1442, 1482, 1587, 2423
BseNI	ACTGG	1 cut(s)	2552
BseRI	GAGGAG	3 cut(s)	125, 188, 1387
BseXI	GCAGC	7 cut(s)	172, 1075, 1104, 1262, 1469, 1733, 2425
BseYI	CCCAGC	2 cut(s)	1276, 1858
BsgI	GTGCAG	3 cut(s)	1838, 2156, 2347
Bsh1236I	CGCG	1 cut(s)	180
BshFI	GGCC	3 cut(s)	1628, 1999, 2672
BshNI	GGYRCC	3 cut(s)	605, 1648, 2317
BshTI	ACCGGT	2 cut(s)	123, 2314
BshVI	ATCGAT	1 cut(s)	620
BsiHKAI	GWGCWC	2 cut(s)	117, 1602
BsiHKCI	CYCGRG	2 cut(s)	2156, 2468
BsiSI	CCGG	5 cut(s)	18, 32, 103, 124, 2315
BslFI	GGGAC	2 cut(s)	41, 2173
BslI	CCNNNNNNNGG	4 cut(s)	109, 2488, 2489, 2558
BsmAI	GTCTC	3 cut(s)	1902, 1987, 2533
BsmBI	CGTCTC	1 cut(s)	2533
BsmFI	GGGAC	2 cut(s)	41, 2173
BsnI	GGCC	3 cut(s)	1628, 1999, 2672
BsoBI	CYCGRG	2 cut(s)	2156, 2468
Bsp119I	TTCGAA	2 cut(s)	524, 1562
Bsp1286I	GDGCHC	3 cut(s)	117, 1602, 1784
Bsp143I	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
Bsp1720I	GCTNAGC	1 cut(s)	1596
Bsp19I	CCATGG	3 cut(s)	763, 901, 1176
BspACI	CCGC	6 cut(s)	59, 163, 545, 2286, 2353, 2633
BspANI	GGCC	3 cut(s)	1628, 1999, 2672
BspCNI	CTCAG	7 cut(s)	250, 448, 1333, 1441, 1483, 1588, 2424
BspDI	ATCGAT	1 cut(s)	620
BspFNI	CGCG	1 cut(s)	180
BspHI	TCATGA	2 cut(s)	507, 1203
BspLI	GGNNCC	8 cut(s)	57, 607, 1650, 1857, 2309, 2319, 2550, 2563
BspMI	ACCTGC	4 cut(s)	526, 790, 1186, 1660
BspOI	GCTAGC	1 cut(s)	2030
BspPI	GGATC	2 cut(s)	1746, 2215
BspQI	GCTCTTC	2 cut(s)	369, 789
BspT104I	TTCGAA	2 cut(s)	524, 1562
BspT107I	GGYRCC	3 cut(s)	605, 1648, 2317
BsrDI	GCAATG	2 cut(s)	1305, 2446
BsrFI	RCCGGY	2 cut(s)	123, 2314
BsrI	ACTGG	1 cut(s)	2552
BssAI	RCCGGY	2 cut(s)	123, 2314
BssECI	CCNNGG	8 cut(s)	763, 901, 1128, 1155, 1176, 1329, 1630, 2565
BssMI	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
BssNI	GRCGYC	2 cut(s)	227, 1265
BssSI	CACGAG	1 cut(s)	1601
BssT1I	CCWWGG	6 cut(s)	763, 901, 1155, 1176, 1630, 2565
Bst2BI	CACGAG	1 cut(s)	1601
Bst2UI	CCWGG	4 cut(s)	119, 1129, 1625, 2305
Bst4CI	ACNGT	6 cut(s)	638, 1330, 1363, 1483, 1591, 2037
Bst6I	CTCTTC	3 cut(s)	369, 789, 1397
BstACI	GRCGYC	2 cut(s)	227, 1265
BstBI	TTCGAA	2 cut(s)	524, 1562
BstC8I	GCNNGC	5 cut(s)	1193, 1657, 2028, 2654, 2670
BstDEI	CTNAG	7 cut(s)	237, 456, 1341, 1428, 1491, 1596, 2432
BstDSI	CCRYGG	4 cut(s)	763, 901, 1176, 1329
BstF5I	GGATG	6 cut(s)	31, 421, 1137, 1186, 1300, 1795
BstFNI	CGCG	1 cut(s)	180
BstH2I	RGCGCY	2 cut(s)	2439, 2659
BstHHI	GCGC	4 cut(s)	180, 550, 2438, 2658
BstKTI	GATC	9 cut(s)	1231, 1741, 1840, 1972, 2223, 2242, 2387, 2431, 2628
BstMAI	GTCTC	3 cut(s)	1902, 1987, 2533
BstMBI	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 145, 233, 1457, 2521, 2641
BstNI	CCWGG	4 cut(s)	119, 1129, 1625, 2305
BstNSI	RCATGY	1 cut(s)	2006
BstSCI	CCNGG	5 cut(s)	101, 117, 1127, 1623, 2303
BstUI	CGCG	1 cut(s)	180
BstV1I	GCAGC	7 cut(s)	172, 1075, 1104, 1262, 1469, 1733, 2425
BstV2I	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
BstX2I	RGATCY	2 cut(s)	1738, 2239
BstXI	CCANNNNNNTGG	2 cut(s)	1631, 2204
BstYI	RGATCY	2 cut(s)	1738, 2239
Bsu15I	ATCGAT	1 cut(s)	620
Bsu36I	CCTNAGG	1 cut(s)	1428
BsuI	GTATCC	1 cut(s)	2626
BsuRI	GGCC	3 cut(s)	1628, 1999, 2672
BsuTUI	ATCGAT	1 cut(s)	620
BtgI	CCRYGG	4 cut(s)	763, 901, 1176, 1329
BtsCI	GGATG	6 cut(s)	31, 421, 1137, 1186, 1300, 1795
BtsI	GCAGTG	2 cut(s)	68, 678
BtsIMutI	CAGTG	7 cut(s)	68, 453, 678, 1338, 1596, 1821, 2042
BveI	ACCTGC	4 cut(s)	526, 790, 1186, 1660
Cac8I	GCNNGC	5 cut(s)	1193, 1657, 2028, 2654, 2670
CaiI	CAGNNNCTG	1 cut(s)	1595
CciI	TCATGA	2 cut(s)	507, 1203
CfoI	GCGC	4 cut(s)	180, 550, 2438, 2658
Cfr10I	RCCGGY	2 cut(s)	123, 2314
Cfr13I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1855, 1997
ClaI	ATCGAT	1 cut(s)	620
CseI	GACGC	2 cut(s)	216, 1254
Csp6I	GTAC	5 cut(s)	639, 1587, 1807, 2033, 2318
CspAI	ACCGGT	2 cut(s)	123, 2314
CspCI	CAANNNNNGTGG	2 cut(s)	1327, 1362
CviAII	CATG	9 cut(s)	508, 764, 902, 1040, 1177, 1204, 1987, 2003, 2193
CviQI	GTAC	5 cut(s)	639, 1587, 1807, 2033, 2318
DdeI	CTNAG	7 cut(s)	237, 456, 1341, 1428, 1491, 1596, 2432
DpnI	GATC	9 cut(s)	1230, 1740, 1839, 1971, 2222, 2241, 2386, 2430, 2627
DpnII	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
DriI	GACNNNNNGTC	1 cut(s)	2166
Eam1104I	CTCTTC	3 cut(s)	369, 789, 1397
Eam1105I	GACNNNNNGTC	1 cut(s)	2166
EarI	CTCTTC	3 cut(s)	369, 789, 1397
Ecl136II	GAGCTC	1 cut(s)	115
Eco130I	CCWWGG	6 cut(s)	763, 901, 1155, 1176, 1630, 2565
Eco24I	GRGCYC	2 cut(s)	117, 1784
Eco32I	GATATC	1 cut(s)	370
Eco47I	GGWCC	4 cut(s)	55, 79, 121, 1855
Eco47III	AGCGCT	1 cut(s)	2437
Eco53kI	GAGCTC	1 cut(s)	115
Eco57I	CTGAAG	3 cut(s)	455, 1244, 1556
Eco81I	CCTNAGG	1 cut(s)	1428
Eco88I	CYCGRG	2 cut(s)	2156, 2468
EcoICRI	GAGCTC	1 cut(s)	115
EcoO109I	RGGNCCY	1 cut(s)	1627
EcoRI	GAATTC	2 cut(s)	394, 2477
EcoRII	CCWGG	4 cut(s)	117, 1127, 1623, 2303
EcoRV	GATATC	1 cut(s)	370
EcoT14I	CCWWGG	6 cut(s)	763, 901, 1155, 1176, 1630, 2565
EcoT38I	GRGCYC	2 cut(s)	117, 1784
ErhI	CCWWGG	6 cut(s)	763, 901, 1155, 1176, 1630, 2565
Esp3I	CGTCTC	1 cut(s)	2533
FaeI	CATG	9 cut(s)	511, 767, 905, 1043, 1180, 1207, 1990, 2006, 2196
FalI	AAGNNNNNCTT	6 cut(s)	59, 91, 912, 944, 2341, 2373
FaqI	GGGAC	2 cut(s)	41, 2173
FatI	CATG	9 cut(s)	507, 763, 901, 1039, 1176, 1203, 1986, 2002, 2192
FauI	CCCGC	2 cut(s)	66, 156
FauNDI	CATATG	1 cut(s)	2110
FblI	GTMKAC	1 cut(s)	2412
Fnu4HI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1722, 2287, 2439
FokI	GGATG	6 cut(s)	38, 428, 1124, 1193, 1307, 1802
FriOI	GRGCYC	2 cut(s)	117, 1784
Fsp4HI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1722, 2287, 2439
FspBI	CTAG	2 cut(s)	1868, 2027
GlaI	GCGC	4 cut(s)	179, 549, 2437, 2657
GluI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1722, 2287, 2439
GsaI	CCCAGC	2 cut(s)	1280, 1862
GsuI	CTGGAG	4 cut(s)	80, 101, 134, 1927
HaeII	RGCGCY	2 cut(s)	2439, 2659
HaeIII	GGCC	3 cut(s)	1628, 1999, 2672
HapII	CCGG	5 cut(s)	18, 32, 103, 124, 2315
HgaI	GACGC	2 cut(s)	216, 1254
HhaI	GCGC	4 cut(s)	180, 550, 2438, 2658
Hin1I	GRCGYC	2 cut(s)	227, 1265
Hin1II	CATG	9 cut(s)	511, 767, 905, 1043, 1180, 1207, 1990, 2006, 2196
Hin6I	GCGC	4 cut(s)	178, 548, 2436, 2656
HinP1I	GCGC	4 cut(s)	178, 548, 2436, 2656
HindIII	AAGCTT	1 cut(s)	2150
HpaII	CCGG	5 cut(s)	18, 32, 103, 124, 2315
HphI	GGTGA	3 cut(s)	1404, 1444, 2582
Hpy166II	GTNNAC	3 cut(s)	641, 1333, 2413
Hpy8I	GTNNAC	3 cut(s)	641, 1333, 2413
HpyCH4III	ACNGT	6 cut(s)	638, 1330, 1363, 1483, 1591, 2037
HpyCH4IV	ACGT	1 cut(s)	2542
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 145, 233, 1457, 2521, 2641
HpyF3I	CTNAG	7 cut(s)	237, 456, 1341, 1428, 1491, 1596, 2432
HpySE526I	ACGT	1 cut(s)	2542
Hsp92I	GRCGYC	2 cut(s)	227, 1265
Hsp92II	CATG	9 cut(s)	511, 767, 905, 1043, 1180, 1207, 1990, 2006, 2196
HspAI	GCGC	4 cut(s)	178, 548, 2436, 2656
KpnI	GGTACC	1 cut(s)	2321
Kzo9I	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
LguI	GCTCTTC	2 cut(s)	369, 789
LmnI	GCTCC	9 cut(s)	99, 112, 120, 136, 153, 175, 1522, 1567, 2567
Lsp1109I	GCAGC	7 cut(s)	172, 1075, 1104, 1262, 1469, 1733, 2425
LweI	GCATC	4 cut(s)	1285, 2050, 2460, 2502
MaeI	CTAG	2 cut(s)	1868, 2027
MaeII	ACGT	1 cut(s)	2542
MaeIII	GTNAC	7 cut(s)	451, 569, 632, 885, 1023, 1389, 2645
MalI	GATC	9 cut(s)	1230, 1740, 1839, 1971, 2222, 2241, 2386, 2430, 2627
MboI	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
MfeI	CAATTG	1 cut(s)	2202
MflI	RGATCY	2 cut(s)	1738, 2239
MhlI	GDGCHC	3 cut(s)	117, 1602, 1784
MlyI	GAGTC	6 cut(s)	83, 762, 1121, 1585, 1820, 2480
MmeI	TCCRAC	3 cut(s)	171, 291, 2511
MroXI	GAANNNNTTC	2 cut(s)	1406, 1830
MseI	TTAA	9 cut(s)	213, 476, 540, 1799, 1884, 2007, 2130, 2327, 2511
MslI	CAYNNNNRTG	1 cut(s)	1800
MspA1I	CMGCKG	4 cut(s)	163, 1460, 1937, 2668
MspI	CCGG	5 cut(s)	18, 32, 103, 124, 2315
MspR9I	CCNGG	5 cut(s)	103, 119, 1129, 1625, 2305
MunI	CAATTG	1 cut(s)	2202
MvaI	CCWGG	4 cut(s)	119, 1129, 1625, 2305
MvnI	CGCG	1 cut(s)	180
MwoI	GCNNNNNNNGC	6 cut(s)	67, 145, 233, 1457, 2521, 2641
NciI	CCSGG	1 cut(s)	103
NcoI	CCATGG	3 cut(s)	763, 901, 1176
NdeI	CATATG	1 cut(s)	2110
NdeII	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
NheI	GCTAGC	1 cut(s)	2026
NlaIII	CATG	9 cut(s)	511, 767, 905, 1043, 1180, 1207, 1990, 2006, 2196
NlaIV	GGNNCC	8 cut(s)	57, 607, 1650, 1857, 2309, 2319, 2550, 2563
NmuCI	GTSAC	2 cut(s)	451, 569
NspI	RCATGY	1 cut(s)	2006
NspV	TTCGAA	2 cut(s)	524, 1562
PaeR7I	CTCGAG	1 cut(s)	2468
PagI	TCATGA	2 cut(s)	507, 1203
PaqCI	CACCTGC	1 cut(s)	1660
PciI	ACATGT	1 cut(s)	2002
PciSI	GCTCTTC	2 cut(s)	369, 789
PcsI	WCGNNNNNNNCGW	1 cut(s)	224
PdmI	GAANNNNTTC	2 cut(s)	1406, 1830
PflMI	CCANNNNNTGG	1 cut(s)	2558
PfoI	TCCNGGA	1 cut(s)	117
PinAI	ACCGGT	2 cut(s)	123, 2314
PkrI	GCNGC	9 cut(s)	162, 547, 1065, 1119, 1252, 1459, 1723, 2288, 2440
PleI	GAGTC	6 cut(s)	83, 762, 1120, 1584, 1819, 2479
PpsI	GAGTC	6 cut(s)	83, 762, 1120, 1584, 1819, 2479
PscI	ACATGT	1 cut(s)	2002
PsiI	TTATAA	1 cut(s)	1712
Psp124BI	GAGCTC	1 cut(s)	117
Psp6I	CCWGG	4 cut(s)	117, 1127, 1623, 2303
PspFI	CCCAGC	2 cut(s)	1276, 1858
PspGI	CCWGG	4 cut(s)	117, 1127, 1623, 2303
PspN4I	GGNNCC	8 cut(s)	57, 607, 1650, 1857, 2309, 2319, 2550, 2563
PspPI	GGNCC	6 cut(s)	55, 79, 121, 1627, 1855, 1997
PstNI	CAGNNNCTG	1 cut(s)	1595
PsuI	RGATCY	2 cut(s)	1738, 2239
PvuII	CAGCTG	3 cut(s)	1460, 1937, 2668
RsaI	GTAC	5 cut(s)	640, 1588, 1808, 2034, 2319
RsaNI	GTAC	5 cut(s)	639, 1587, 1807, 2033, 2318
RseI	CAYNNNNRTG	1 cut(s)	1800
SacI	GAGCTC	1 cut(s)	117
SapI	GCTCTTC	2 cut(s)	369, 789
SaqAI	TTAA	9 cut(s)	213, 476, 540, 1799, 1884, 2007, 2130, 2327, 2511
SatI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1722, 2287, 2439
Sau3AI	GATC	9 cut(s)	1228, 1738, 1837, 1969, 2220, 2239, 2384, 2428, 2625
Sau96I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1855, 1997
SchI	GAGTC	6 cut(s)	83, 762, 1121, 1585, 1820, 2480
ScrFI	CCNGG	5 cut(s)	103, 119, 1129, 1625, 2305
SduI	GDGCHC	3 cut(s)	117, 1602, 1784
SfaNI	GCATC	4 cut(s)	1285, 2050, 2460, 2502
Sfr274I	CTCGAG	1 cut(s)	2468
SfuI	TTCGAA	2 cut(s)	524, 1562
SinI	GGWCC	4 cut(s)	55, 79, 121, 1855
SlaI	CTCGAG	1 cut(s)	2468
SmiMI	CAYNNNNRTG	1 cut(s)	1800
SmlI	CTYRAG	3 cut(s)	929, 1011, 2468
SmoI	CTYRAG	3 cut(s)	929, 1011, 2468
SpeI	ACTAGT	1 cut(s)	1867
SsiI	CCGC	6 cut(s)	59, 163, 545, 2286, 2353, 2633
SspMI	CTAG	2 cut(s)	1868, 2027
SstI	GAGCTC	1 cut(s)	117
StyD4I	CCNGG	5 cut(s)	101, 117, 1127, 1623, 2303
StyI	CCWWGG	6 cut(s)	763, 901, 1155, 1176, 1630, 2565
TaaI	ACNGT	6 cut(s)	638, 1330, 1363, 1483, 1591, 2037
TaiI	ACGT	1 cut(s)	2545
TatI	WGTACW	1 cut(s)	2032
TauI	GCSGC	2 cut(s)	548, 2289
Tru1I	TTAA	9 cut(s)	213, 476, 540, 1799, 1884, 2007, 2130, 2327, 2511
Tru9I	TTAA	9 cut(s)	213, 476, 540, 1799, 1884, 2007, 2130, 2327, 2511
TscAI	CASTG	7 cut(s)	68, 460, 678, 1345, 1596, 1821, 2042
TseFI	GTSAC	2 cut(s)	451, 569
TseI	GCWGC	7 cut(s)	160, 1063, 1117, 1250, 1457, 1721, 2438
Tsp45I	GTSAC	2 cut(s)	451, 569
TspGWI	ACGGA	4 cut(s)	398, 480, 1802, 1991
TspRI	CASTG	7 cut(s)	68, 460, 678, 1345, 1596, 1821, 2042
Van91I	CCANNNNNTGG	1 cut(s)	2558
VpaK11BI	GGWCC	4 cut(s)	55, 79, 121, 1855
XapI	RAATTY	7 cut(s)	49, 394, 692, 968, 1771, 2068, 2477
XceI	RCATGY	1 cut(s)	2006
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XhoI	CTCGAG	1 cut(s)	2468
XmiI	GTMKAC	1 cut(s)	2412
XmnI	GAANNNNTTC	2 cut(s)	1406, 1830
XspI	CTAG	2 cut(s)	1868, 2027

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.