Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

29767376 .. 29772476

Gene Structure 5101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1214500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2994 bp

ATGGAAGATAAAGGTTTGAAGTTGGAAGAATCTGAGTTGAGGCAGGGAAAGCTGGCCCTGCAATTTGAGGGCCTTTTGGACTGTATTGATTCCGCCCGGAACGAGATCCTTGGCCTATTTCAAGAGGTCCAGGATAAGGTTAAGAAAATTGGAGTTTTACAGAGCCAAGTCGAAGTCAAATCTGAGGAATTGCGTGGAATTGAGAGGTTGATTGAAAAGAAATCAAATGAGCTCGAATCACTCGAGTCCTCCTTGTGGGACTGTCAGTCATCGATTGAAGAGCACAATGAGGAGCTTTCTGCTAAGGTGAAGCGGCTGGAAGAAGTTCAGAGATGGGTTAGGGAGAATGAGAGGGAGTATGATTCGATCAAAAAAGGGATTGAACATGGAAGAAGTAAATTGAATTGGTATGAGTCCAAGGTGGAAAAATTGAAAGGGGTTCTACGAACATTAGAGATATACAATGAGGAAATTGAGTTTCAAAAGAAGCGATTAAATGAGATTCGGGGGTCAATTGAGGAGCAGAAGGAAGAACTTGCTTTGAAAAAGGAGCAGACTAAAGAGGCGGAAAGAAGGGTTGATCAATGTGACAGAGAGACGAAGGATAAAGAGAAGAAACTTAGTACGATTAAGAAGTCGATTGAGGAATCCTCTAAGAAAATTGAATCAAGAGAGAAAATTATTGATGAAATGGATTTGAAAGTGAAAGATTTTTTCTTGCTGAAGAAATCCATGGAGGAGTGGTCCACCAAACTCGAATTGAAAGAGAGAGAATTCGAGTCCAAAGTTGAGGATCTTACTTTGATTCATAAGAGAGTGGATGAATGCGTCAATGAGGTTCAGTTGAAGGAACAACATTTGGATTCACTTGAAAAATTGATTCGAGAAGGAAAAATGAATTTGGATTCACAAGAAAGGTTGTTAAAAGAATTCTCCAATGGTCTTGAATTAAAAGAAAGGCAACTTGAGCAGCGGGATAAAGAACTTGAATCAAAACGACAAGATATTGATTCGATCCGAAATTCTACCCATGCTAACAGTGTCATTGTTTCTTCTTCCACAAGTAATCACTTCAGCATCGATAGGGATGGTGGAGGCTTGCAGTTACTCATGAAAGAGAGTGTAAAGAGAATTGATTTATTGAGTAGTGAATTGGCAGCTTTTCTTCAAGCGTCATCAGACCCAGCAAAATTGGTTTTGGATGCAATGCAAGGGTTTTACCATTCAAATTCGATTCAGGACAACAAGGGGTTTGATTATGATCTGACAGTTATAAGAAGAAGTTGTGTTCTTTTGTTACAGGAGTTGAAGAGGTTATCACCACCGATCAATCCTCCGGTGAGAGAACAAGCAATTAAGTTGGTGGATGACTGGAAGGAAAAGATGACAGTGGTGGTGGAAAATAGTTTGGAGGTTTTGGGTCTTTTGTGGCTTCTTACAGCATTTGAGTTGACCTCAACTTATGATGCGAGGGAGCTTCAAAGTCTTCTTGCTTTAGTTTCTCACCCAGAACATGCACCTCAACTGCGCCAGGCCCTTGGCATCACATACAATGCATCTGTATATGGCGCCCTGTCAGTCCCTGTCAAAATTGAGGAACCAGAACCTTCAACAGTCAGAAATGTAGCTACTTCTCCTTCTCCAATTCTTCAACTAAGTGCAACTGCCACCACAGATGCAAGGAATTCGCAGGACTGTCTAGATGAGCATTTAAGTGGGAATGACTCAGCTAATAACAAATTGCTTGATGTTCTACGACTGCCAGTAGACCGAGAAAAAGTAGTTTTGAAGCTGATGCAAAAGTCTCTTGATCACTACTGGAGCAATAGAAATGTTGGTTTCATAGCCAATCTCAAGAAGACCTACATTCCCATGTTGAATGAGCTGATGAAAATCTCAACAAATGTTGCCCTTGATGTGAAAGAAGATGCAGTCAAGCTAGCAGTCCAGTGGCAAGCAAAAATGAGAACCGATACAGAAAACTCGTGGGAGATTTTGGGTTTTTTGCAATTTGTGGCTGCATATGGATTGCTTTCTACCTTGAGTGTAGATGAGACTGTGATGCTTCTTGGAAACATATGTCAGAATAAACCGGTCCTACAATTATGTCGGATACTTAGTTTTGCAGATAAGATCCCCGGCTTTATTCGGGATCTGATTGAAAGGAAACAGATGATTAAGGCTGTTAGATTGATTTGCACCTTCAAGTTAACTGACAGGTTCCCGCCAGTAGTACTCTTAAATAATTATGTGGAGGATCTAAGGAAGTCTTTCCGTGCAATATTCCCGGGAAAGAAAAGAATTTATGAAAAGGATAATGTAATGAACAACCTAATAGCTGATCTGAGAGGTGTACTTCAGTGCATAAAGGATCACAATCTTGAGTCCGAATATCCATATAAGGACATTGAATTGGAAATAGTTCAGCTGGAAAGACTAAAGGAGAATTTGAGATTCTTGCCGACGGGGATTGCCTCCAGGTGTGAACAGCAAGAACAGGAAAGAAGGAAGAGATGCGGTACTAGTTCTACCGCCCAGTTTCAACCACCAGAGAAACGTCAAAATAGAAGTCATCCGACAGCTCTAGCAGCTGCTGCTAGATCCTACACAACTTTCCGTTCGCAGTTAAATCCGTCATCTCAGCTATTTGGAAATAATGATGGACACCCTGGGCAGTTTAGCATGTTTGCCAACCACCATGAAACTGGTTGCAGTTTTGTTTCCGAGCAGTTAACTTCATCGTCTCAGCTGTATGAAAACTATGGACACCCTTTGCAGTTTGCCATGGACCATGAAATCGGTGCTAATTTTGGTCCCATGCAGTTAAGGTCGTCCTCTCAGCTGGCTGAAAATTTTGGATTACCTGCGCCGCTTCCTTTGGCTTCCAACGAGCATGAGATTGGCACTGATTTCAGTTGTATGCAGGATGCCGGATTATATAACTCTAATCAGTTCTTGCCCGGCACTCATGGATTTGGCCGTAGAGGAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

998

Amino Acids

114.16

Weight (kDa)

5.77

Isoelectric Point (pI)

53.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	357 - 516	2.5e-29	Frigida-like protein
Frigida	PF07899	559 - 816	3.1e-50	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1274
Acc36I	ACCTGC	1 cut(s)	2872
AccB1I	GGYRCC	1 cut(s)	1568
AccI	GTMKAC	1 cut(s)	1767
AciI	CCGC	8 cut(s)	93, 313, 566, 973, 2225, 2517, 2532, 2870
AclWI	GGATC	8 cut(s)	100, 801, 1009, 2128, 2160, 2265, 2379, 2595
AcoI	YGGCCR	1 cut(s)	2977
AcsI	RAATTY	9 cut(s)	773, 898, 929, 1021, 1228, 1684, 2301, 2446, 2851
AcuI	CTGAAG	3 cut(s)	743, 1057, 2342
AcyI	GRCGYC	1 cut(s)	1569
AfaI	GTAC	4 cut(s)	625, 2235, 2355, 2521
AfiI	CCNNNNNNNGG	2 cut(s)	136, 255
AgeI	ACCGGT	1 cut(s)	2092
AhdI	GACNNNNNGTC	1 cut(s)	265
AhlI	ACTAGT	1 cut(s)	2522
AjnI	CCWGG	4 cut(s)	129, 1530, 2477, 2668
Alw21I	GWGCWC	2 cut(s)	234, 285
Alw26I	GTCTC	4 cut(s)	590, 1809, 2048, 2748
AlwI	GGATC	8 cut(s)	100, 801, 1009, 2128, 2160, 2265, 2379, 2595
AlwNI	CAGNNNCTG	2 cut(s)	1583, 2594
Ama87I	CYCGRG	2 cut(s)	242, 2287
AoxI	GGCC	5 cut(s)	54, 70, 112, 1533, 2977
ApeKI	GCWGC	6 cut(s)	970, 1157, 2018, 2588, 2591, 2594
ApoI	RAATTY	9 cut(s)	773, 898, 929, 1021, 1228, 1684, 2301, 2446, 2851
ArsI	GACNNNNNNTTYG	2 cut(s)	2554, 2586
AsiGI	ACCGGT	1 cut(s)	2092
Asp700I	GAANNNNTTC	3 cut(s)	324, 2270, 2421
AspLEI	GCGC	3 cut(s)	1530, 1571, 2869
AspS9I	GGNCC	8 cut(s)	55, 70, 127, 744, 1534, 2095, 2788, 2813
AsuC2I	CCSGG	5 cut(s)	97, 2139, 2288, 2289, 2961
AsuHPI	GGTGA	4 cut(s)	319, 1311, 1351, 1496
AsuNHI	GCTAGC	1 cut(s)	1939
AvaI	CYCGRG	2 cut(s)	242, 2287
AvaII	GGWCC	5 cut(s)	127, 744, 2095, 2788, 2813
BanI	GGYRCC	1 cut(s)	1568
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	1 cut(s)	1984
BbsI	GAAGAC	2 cut(s)	1478, 1865
Bbv12I	GWGCWC	2 cut(s)	234, 285
BbvI	GCAGC	6 cut(s)	982, 1169, 2005, 2578, 2581, 2600
BccI	CCATC	3 cut(s)	327, 1082, 2654
BceAI	ACGGC	1 cut(s)	2964
BcgI	CGANNNNNNTGC	2 cut(s)	1668, 1702
BciT130I	CCWGG	4 cut(s)	131, 1532, 2479, 2670
BciVI	GTATCC	1 cut(s)	2106
BclI	TGATCA	2 cut(s)	580, 1810
BcnI	CCSGG	5 cut(s)	97, 2139, 2288, 2289, 2961
BcoDI	GTCTC	4 cut(s)	590, 1809, 2048, 2748
BcuI	ACTAGT	1 cut(s)	2522
BfaI	CTAG	6 cut(s)	1700, 1940, 2523, 2585, 2598, 2992
BfoI	RGCGCY	1 cut(s)	1572
BfuAI	ACCTGC	1 cut(s)	2872
BfuI	GTATCC	1 cut(s)	2106
BisI	GCNGC	8 cut(s)	314, 971, 1158, 2019, 2589, 2592, 2595, 2870
BlsI	GCNGC	8 cut(s)	315, 972, 1159, 2020, 2590, 2593, 2596, 2871
BmcAI	AGTACT	1 cut(s)	2235
Bme1390I	CCNGG	9 cut(s)	97, 131, 1532, 2139, 2288, 2289, 2479, 2670, 2961
Bme18I	GGWCC	5 cut(s)	127, 744, 2095, 2788, 2813
BmeRI	GACNNNNNGTC	1 cut(s)	265
BmeT110I	CYCGRG	2 cut(s)	242, 2287
BmgT120I	GGNCC	8 cut(s)	55, 70, 127, 744, 1534, 2095, 2788, 2813
BmiI	GGNNCC	5 cut(s)	1570, 1599, 2222, 2815, 2989
BmrFI	CCNGG	9 cut(s)	97, 131, 1532, 2139, 2288, 2289, 2479, 2670, 2961
BmrI	ACTGGG	1 cut(s)	2530
BmtI	GCTAGC	1 cut(s)	1943
BmuI	ACTGGG	1 cut(s)	2530
BpiI	GAAGAC	2 cut(s)	1478, 1865
BplI	GAGNNNNNCTC	4 cut(s)	635, 667, 1439, 1471
BpmI	CTGGAG	2 cut(s)	1840, 2461
Bpu10I	CCTNAGC	1 cut(s)	303
BpuEI	CTTGAG	4 cut(s)	986, 1838, 2062, 2402
BpuMI	CCSGG	5 cut(s)	97, 2139, 2288, 2289, 2961
Bsa29I	ATCGAT	2 cut(s)	272, 1080
BsaBI	GATNNNNATC	3 cut(s)	1260, 1892, 2376
BsaHI	GRCGYC	1 cut(s)	1569
BsaJI	CCNNGG	9 cut(s)	109, 417, 732, 1537, 2137, 2287, 2668, 2669, 2784
BsaWI	WCCGGW	2 cut(s)	1336, 2092
BsaXI	ACNNNNNCTCC	2 cut(s)	728, 758
Bsc4I	CCNNNNNNNGG	2 cut(s)	136, 255
Bse118I	RCCGGY	1 cut(s)	2092
Bse1I	ACTGG	7 cut(s)	1376, 1763, 1823, 1948, 2228, 2536, 2710
Bse3DI	GCAATG	1 cut(s)	1212
Bse8I	GATNNNNATC	3 cut(s)	1260, 1892, 2376
BseBI	CCWGG	4 cut(s)	131, 1532, 2479, 2670
BseCI	ATCGAT	2 cut(s)	272, 1080
BseDI	CCNNGG	9 cut(s)	109, 417, 732, 1537, 2137, 2287, 2668, 2669, 2784
BseGI	GGATG	6 cut(s)	826, 1093, 1207, 1372, 2572, 2932
BseJI	GATNNNNATC	3 cut(s)	1260, 1892, 2376
BseLI	CCNNNNNNNGG	2 cut(s)	136, 255
BseMI	GCAATG	1 cut(s)	1212
BseMII	CTCAG	7 cut(s)	24, 174, 1740, 2336, 2654, 2759, 2852
BseNI	ACTGG	7 cut(s)	1376, 1763, 1823, 1948, 2228, 2536, 2710
BseRI	GAGGAG	3 cut(s)	305, 533, 752
BseXI	GCAGC	6 cut(s)	982, 1169, 2005, 2578, 2581, 2600
BseYI	CCCAGC	1 cut(s)	1183
BshFI	GGCC	5 cut(s)	56, 72, 114, 1535, 2979
BshNI	GGYRCC	1 cut(s)	1568
BshTI	ACCGGT	1 cut(s)	2092
BshVI	ATCGAT	2 cut(s)	272, 1080
BsiHKAI	GWGCWC	2 cut(s)	234, 285
BsiHKCI	CYCGRG	2 cut(s)	242, 2287
BsiSI	CCGG	7 cut(s)	97, 1337, 2093, 2139, 2288, 2931, 2961
BslFI	GGGAC	3 cut(s)	272, 1565, 2799
BslI	CCNNNNNNNGG	2 cut(s)	136, 255
BsmAI	GTCTC	4 cut(s)	590, 1809, 2048, 2748
BsmBI	CGTCTC	2 cut(s)	590, 2748
BsmFI	GGGAC	3 cut(s)	272, 1565, 2799
BsmI	GAATGC	1 cut(s)	830
BsnI	GGCC	5 cut(s)	56, 72, 114, 1535, 2979
BsoBI	CYCGRG	2 cut(s)	242, 2287
Bsp1286I	GDGCHC	2 cut(s)	234, 285
Bsp19I	CCATGG	2 cut(s)	732, 2784
BspACI	CCGC	8 cut(s)	93, 313, 566, 973, 2225, 2517, 2532, 2870
BspANI	GGCC	5 cut(s)	56, 72, 114, 1535, 2979
BspCNI	CTCAG	7 cut(s)	25, 175, 1739, 2337, 2653, 2758, 2851
BspDI	ATCGAT	2 cut(s)	272, 1080
BspHI	TCATGA	1 cut(s)	1110
BspLI	GGNNCC	5 cut(s)	1570, 1599, 2222, 2815, 2989
BspMI	ACCTGC	1 cut(s)	2872
BspOI	GCTAGC	1 cut(s)	1943
BspPI	GGATC	8 cut(s)	100, 801, 1009, 2128, 2160, 2265, 2379, 2595
BspQI	GCTCTTC	1 cut(s)	273
BspT107I	GGYRCC	1 cut(s)	1568
BsrDI	GCAATG	1 cut(s)	1212
BsrFI	RCCGGY	1 cut(s)	2092
BsrI	ACTGG	7 cut(s)	1376, 1763, 1823, 1948, 2228, 2536, 2710
BssAI	RCCGGY	1 cut(s)	2092
BssECI	CCNNGG	9 cut(s)	109, 417, 732, 1537, 2137, 2287, 2668, 2669, 2784
BssNI	GRCGYC	1 cut(s)	1569
BssSI	CACGAG	1 cut(s)	1984
BssT1I	CCWWGG	5 cut(s)	109, 417, 732, 1537, 2784
Bst2BI	CACGAG	1 cut(s)	1984
Bst2UI	CCWGG	4 cut(s)	131, 1532, 2479, 2670
Bst4CI	ACNGT	8 cut(s)	83, 263, 1040, 1270, 1390, 1615, 1697, 2059
Bst6I	CTCTTC	3 cut(s)	273, 1304, 2504
BstACI	GRCGYC	1 cut(s)	1569
BstAPI	GCANNNNNTGC	1 cut(s)	2594
BstC8I	GCNNGC	5 cut(s)	54, 1100, 1941, 1956, 2844
BstDSI	CCRYGG	2 cut(s)	732, 2784
BstF5I	GGATG	6 cut(s)	826, 1093, 1207, 1372, 2572, 2932
BstH2I	RGCGCY	1 cut(s)	1572
BstHHI	GCGC	3 cut(s)	1530, 1571, 2869
BstMAI	GTCTC	4 cut(s)	590, 1809, 2048, 2748
BstMWI	GCNNNNNNNGC	5 cut(s)	49, 58, 967, 2588, 2594
BstNI	CCWGG	4 cut(s)	131, 1532, 2479, 2670
BstNSI	RCATGY	2 cut(s)	1517, 2686
BstSCI	CCNGG	9 cut(s)	95, 129, 1530, 2137, 2286, 2287, 2477, 2668, 2959
BstV1I	GCAGC	6 cut(s)	982, 1169, 2005, 2578, 2581, 2600
BstV2I	GAAGAC	2 cut(s)	1478, 1865
BstX2I	RGATCY	6 cut(s)	105, 793, 2133, 2152, 2257, 2600
BstXI	CCANNNNNNTGG	2 cut(s)	1538, 2705
BstYI	RGATCY	6 cut(s)	105, 793, 2133, 2152, 2257, 2600
Bsu15I	ATCGAT	2 cut(s)	272, 1080
BsuI	GTATCC	1 cut(s)	2106
BsuRI	GGCC	5 cut(s)	56, 72, 114, 1535, 2979
BsuTUI	ATCGAT	2 cut(s)	272, 1080
BtgI	CCRYGG	2 cut(s)	732, 2784
BtsCI	GGATG	6 cut(s)	826, 1093, 1207, 1372, 2572, 2932
BtsIMutI	CAGTG	5 cut(s)	1045, 1395, 1955, 2366, 2904
BveI	ACCTGC	1 cut(s)	2872
Cac8I	GCNNGC	5 cut(s)	54, 1100, 1941, 1956, 2844
CaiI	CAGNNNCTG	2 cut(s)	1583, 2594
CciI	TCATGA	1 cut(s)	1110
CfoI	GCGC	3 cut(s)	1530, 1571, 2869
Cfr10I	RCCGGY	1 cut(s)	2092
Cfr13I	GGNCC	8 cut(s)	55, 70, 127, 744, 1534, 2095, 2788, 2813
Cfr9I	CCCGGG	1 cut(s)	2287
ClaI	ATCGAT	2 cut(s)	272, 1080
CseI	GACGC	2 cut(s)	817, 1161
Csp6I	GTAC	4 cut(s)	624, 2234, 2354, 2520
CspAI	ACCGGT	1 cut(s)	2092
CviQI	GTAC	4 cut(s)	624, 2234, 2354, 2520
DinI	GGCGCC	1 cut(s)	1570
DriI	GACNNNNNGTC	1 cut(s)	265
EaeI	YGGCCR	1 cut(s)	2977
Eam1104I	CTCTTC	3 cut(s)	273, 1304, 2504
Eam1105I	GACNNNNNGTC	1 cut(s)	265
EarI	CTCTTC	3 cut(s)	273, 1304, 2504
EciI	GGCGGA	2 cut(s)	82, 581
Ecl136II	GAGCTC	1 cut(s)	232
Eco130I	CCWWGG	5 cut(s)	109, 417, 732, 1537, 2784
Eco24I	GRGCYC	1 cut(s)	234
Eco47I	GGWCC	5 cut(s)	127, 744, 2095, 2788, 2813
Eco53kI	GAGCTC	1 cut(s)	232
Eco57I	CTGAAG	3 cut(s)	743, 1057, 2342
Eco88I	CYCGRG	2 cut(s)	242, 2287
EcoICRI	GAGCTC	1 cut(s)	232
EcoO109I	RGGNCCY	2 cut(s)	70, 1534
EcoRI	GAATTC	3 cut(s)	773, 929, 1684
EcoRII	CCWGG	4 cut(s)	129, 1530, 2477, 2668
EcoT14I	CCWWGG	5 cut(s)	109, 417, 732, 1537, 2784
EcoT22I	ATGCAT	1 cut(s)	1558
EcoT38I	GRGCYC	1 cut(s)	234
EgeI	GGCGCC	1 cut(s)	1570
EheI	GGCGCC	1 cut(s)	1570
ErhI	CCWWGG	5 cut(s)	109, 417, 732, 1537, 2784
Esp3I	CGTCTC	2 cut(s)	590, 2748
FalI	AAGNNNNNCTT	2 cut(s)	2254, 2286
FaqI	GGGAC	3 cut(s)	272, 1565, 2799
FauI	CCCGC	2 cut(s)	966, 2232
FauNDI	CATATG	2 cut(s)	2023, 2078
FbaI	TGATCA	2 cut(s)	580, 1810
FblI	GTMKAC	1 cut(s)	1767
Fnu4HI	GCNGC	8 cut(s)	314, 971, 1158, 2019, 2589, 2592, 2595, 2870
FokI	GGATG	6 cut(s)	833, 1100, 1214, 1379, 2559, 2939
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	8 cut(s)	314, 971, 1158, 2019, 2589, 2592, 2595, 2870
FspBI	CTAG	6 cut(s)	1700, 1940, 2523, 2585, 2598, 2992
GlaI	GCGC	3 cut(s)	1529, 1570, 2868
GluI	GCNGC	8 cut(s)	314, 971, 1158, 2019, 2589, 2592, 2595, 2870
GsaI	CCCAGC	1 cut(s)	1187
GsuI	CTGGAG	2 cut(s)	1840, 2461
HaeII	RGCGCY	1 cut(s)	1572
HaeIII	GGCC	5 cut(s)	56, 72, 114, 1535, 2979
HapII	CCGG	7 cut(s)	97, 1337, 2093, 2139, 2288, 2931, 2961
HgaI	GACGC	2 cut(s)	817, 1161
HhaI	GCGC	3 cut(s)	1530, 1571, 2869
Hin1I	GRCGYC	1 cut(s)	1569
Hin6I	GCGC	3 cut(s)	1528, 1569, 2867
HinP1I	GCGC	3 cut(s)	1528, 1569, 2867
HincII	GTYRAC	3 cut(s)	1452, 2211, 2733
HindII	GTYRAC	3 cut(s)	1452, 2211, 2733
HpaI	GTTAAC	2 cut(s)	2211, 2733
HpaII	CCGG	7 cut(s)	97, 1337, 2093, 2139, 2288, 2931, 2961
HphI	GGTGA	4 cut(s)	319, 1311, 1351, 1496
Hpy166II	GTNNAC	7 cut(s)	747, 1452, 1768, 2211, 2354, 2486, 2733
Hpy8I	GTNNAC	7 cut(s)	747, 1452, 1768, 2211, 2354, 2486, 2733
Hpy99I	CGWCG	1 cut(s)	2467
HpyCH4III	ACNGT	8 cut(s)	83, 263, 1040, 1270, 1390, 1615, 1697, 2059
HpyCH4IV	ACGT	1 cut(s)	2557
HpyF10VI	GCNNNNNNNGC	5 cut(s)	49, 58, 967, 2588, 2594
HpySE526I	ACGT	1 cut(s)	2557
Hsp92I	GRCGYC	1 cut(s)	1569
HspAI	GCGC	3 cut(s)	1528, 1569, 2867
KasI	GGCGCC	1 cut(s)	1568
Ksp22I	TGATCA	2 cut(s)	580, 1810
KspAI	GTTAAC	2 cut(s)	2211, 2733
LguI	GCTCTTC	1 cut(s)	273
LmnI	GCTCC	5 cut(s)	292, 520, 550, 1474, 1821
Lsp1109I	GCAGC	6 cut(s)	982, 1169, 2005, 2578, 2581, 2600
MaeI	CTAG	6 cut(s)	1700, 1940, 2523, 2585, 2598, 2992
MaeII	ACGT	1 cut(s)	2557
MaeIII	GTNAC	3 cut(s)	587, 1104, 1296
MfeI	CAATTG	1 cut(s)	513
MflI	RGATCY	6 cut(s)	105, 793, 2133, 2152, 2257, 2600
MhlI	GDGCHC	2 cut(s)	234, 285
Mly113I	GGCGCC	1 cut(s)	1569
MlyI	GAGTC	5 cut(s)	254, 422, 788, 1718, 2393
MmeI	TCCRAC	3 cut(s)	2090, 2600, 2910
Mph1103I	ATGCAT	1 cut(s)	1558
MroXI	GAANNNNTTC	3 cut(s)	324, 2270, 2421
MslI	CAYNNNNRTG	2 cut(s)	1713, 1871
MspA1I	CMGCKG	5 cut(s)	973, 2428, 2591, 2749, 2842
MspI	CCGG	7 cut(s)	97, 1337, 2093, 2139, 2288, 2931, 2961
MspR9I	CCNGG	9 cut(s)	97, 131, 1532, 2139, 2288, 2289, 2479, 2670, 2961
MunI	CAATTG	1 cut(s)	513
Mva1269I	GAATGC	1 cut(s)	830
MvaI	CCWGG	4 cut(s)	131, 1532, 2479, 2670
MwoI	GCNNNNNNNGC	5 cut(s)	49, 58, 967, 2588, 2594
NarI	GGCGCC	1 cut(s)	1569
NciI	CCSGG	5 cut(s)	97, 2139, 2288, 2289, 2961
NcoI	CCATGG	2 cut(s)	732, 2784
NdeI	CATATG	2 cut(s)	2023, 2078
NheI	GCTAGC	1 cut(s)	1939
NlaIV	GGNNCC	5 cut(s)	1570, 1599, 2222, 2815, 2989
NmuCI	GTSAC	1 cut(s)	587
NsiI	ATGCAT	1 cut(s)	1558
NspI	RCATGY	2 cut(s)	1517, 2686
PaeR7I	CTCGAG	1 cut(s)	242
PagI	TCATGA	1 cut(s)	1110
PasI	CCCWGGG	1 cut(s)	2669
PciSI	GCTCTTC	1 cut(s)	273
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PctI	GAATGC	1 cut(s)	830
PdmI	GAANNNNTTC	3 cut(s)	324, 2270, 2421
PfoI	TCCNGGA	1 cut(s)	129
PinAI	ACCGGT	1 cut(s)	2092
PkrI	GCNGC	8 cut(s)	315, 972, 1159, 2020, 2590, 2593, 2596, 2871
PleI	GAGTC	5 cut(s)	253, 421, 787, 1718, 2392
PluTI	GGCGCC	1 cut(s)	1572
PpsI	GAGTC	5 cut(s)	253, 421, 787, 1718, 2392
PsiI	TTATAA	1 cut(s)	1274
Psp124BI	GAGCTC	1 cut(s)	234
Psp6I	CCWGG	4 cut(s)	129, 1530, 2477, 2668
PspFI	CCCAGC	1 cut(s)	1183
PspGI	CCWGG	4 cut(s)	129, 1530, 2477, 2668
PspN4I	GGNNCC	5 cut(s)	1570, 1599, 2222, 2815, 2989
PspPI	GGNCC	8 cut(s)	55, 70, 127, 744, 1534, 2095, 2788, 2813
PspXI	VCTCGAGB	1 cut(s)	242
PstNI	CAGNNNCTG	2 cut(s)	1583, 2594
PsuI	RGATCY	6 cut(s)	105, 793, 2133, 2152, 2257, 2600
PvuII	CAGCTG	4 cut(s)	2428, 2591, 2749, 2842
RsaI	GTAC	4 cut(s)	625, 2235, 2355, 2521
RsaNI	GTAC	4 cut(s)	624, 2234, 2354, 2520
RseI	CAYNNNNRTG	2 cut(s)	1713, 1871
SacI	GAGCTC	1 cut(s)	234
SapI	GCTCTTC	1 cut(s)	273
SatI	GCNGC	8 cut(s)	314, 971, 1158, 2019, 2589, 2592, 2595, 2870
Sau96I	GGNCC	8 cut(s)	55, 70, 127, 744, 1534, 2095, 2788, 2813
ScaI	AGTACT	1 cut(s)	2235
SchI	GAGTC	5 cut(s)	254, 422, 788, 1718, 2393
ScrFI	CCNGG	9 cut(s)	97, 131, 1532, 2139, 2288, 2289, 2479, 2670, 2961
SduI	GDGCHC	2 cut(s)	234, 285
SfoI	GGCGCC	1 cut(s)	1570
Sfr274I	CTCGAG	1 cut(s)	242
SinI	GGWCC	5 cut(s)	127, 744, 2095, 2788, 2813
SlaI	CTCGAG	1 cut(s)	242
SmaI	CCCGGG	1 cut(s)	2289
SmiMI	CAYNNNNRTG	2 cut(s)	1713, 1871
SmlI	CTYRAG	5 cut(s)	242, 965, 1853, 2041, 2381
SmoI	CTYRAG	5 cut(s)	242, 965, 1853, 2041, 2381
SpeI	ACTAGT	1 cut(s)	2522
SsiI	CCGC	8 cut(s)	93, 313, 566, 973, 2225, 2517, 2532, 2870
SspDI	GGCGCC	1 cut(s)	1568
SspI	AATATT	1 cut(s)	2283
SspMI	CTAG	6 cut(s)	1700, 1940, 2523, 2585, 2598, 2992
SstI	GAGCTC	1 cut(s)	234
StyD4I	CCNGG	9 cut(s)	95, 129, 1530, 2137, 2286, 2287, 2477, 2668, 2959
StyI	CCWWGG	5 cut(s)	109, 417, 732, 1537, 2784
TaaI	ACNGT	8 cut(s)	83, 263, 1040, 1270, 1390, 1615, 1697, 2059
TaiI	ACGT	1 cut(s)	2560
TaqII	GACCGA	1 cut(s)	1785
TatI	WGTACW	2 cut(s)	2233, 2353
TauI	GCSGC	2 cut(s)	316, 2872
TscAI	CASTG	5 cut(s)	1045, 1395, 1955, 2366, 2911
TseFI	GTSAC	1 cut(s)	587
TseI	GCWGC	6 cut(s)	970, 1157, 2018, 2588, 2591, 2594
Tsp45I	GTSAC	1 cut(s)	587
TspGWI	ACGGA	3 cut(s)	2264, 2606, 2622
TspMI	CCCGGG	1 cut(s)	2287
TspRI	CASTG	5 cut(s)	1045, 1395, 1955, 2366, 2911
VpaK11BI	GGWCC	5 cut(s)	127, 744, 2095, 2788, 2813
XapI	RAATTY	9 cut(s)	773, 898, 929, 1021, 1228, 1684, 2301, 2446, 2851
XbaI	TCTAGA	1 cut(s)	1699
XceI	RCATGY	2 cut(s)	1517, 2686
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2702
XhoI	CTCGAG	1 cut(s)	242
XmaI	CCCGGG	1 cut(s)	2287
XmiI	GTMKAC	1 cut(s)	1767
XmnI	GAANNNNTTC	3 cut(s)	324, 2270, 2421
XspI	CTAG	6 cut(s)	1700, 1940, 2523, 2585, 2598, 2992
ZrmI	AGTACT	1 cut(s)	2235
Zsp2I	ATGCAT	1 cut(s)	1558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.