Rmu_co8146304.1_g000001

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8146304.1
OG0003973
Physical Location & Seq
Reverse (-)
2 .. 516
515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8146304.1_g000001.1.cds

Sequence Viewer

Length: 515 bp
atggacaacatagcctctgatttagacctcgcagagacgaagcagagcaatctgagcagagcctatgagagcctacacacccaagcttctccctttatggtcctaggtcttcagtggaaggacctcgaggaccacctcgactcgacccggaccaaaatccagacccggttccaggagctccaggcccgggaagcccaaatcgccgaccaggtgaaaaagctcgagtctttgaaggaggttgagtacggtaagagtcacttgcagtctttaaagttgttaattgaagaaactagtgaagagcttggtgtgaaagagaagaggttttcggaagttgagaggttgattagagtgaaggagagaatgcttgaggataggaataagatgttgagtttggttgagaggaggatagaggagaagggtagggaggttgaggggaaggagaagaaggtgagggtggtgcaaggagtgttggataagtactgtgttgatattaggaagaggaagaaggagtgtgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

172

Amino Acids

20.16

Weight (kDa)

7.73

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 95
AfaI GTAC 2 cut(s) 245, 479
AfiI CCNNNNNNNGG 2 cut(s) 172, 187
AgsI TTSAA 2 cut(s) 232, 284
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 3 cut(s) 171, 180, 207
AluBI AGCT 4 cut(s) 86, 178, 220, 301
AluI AGCT 4 cut(s) 86, 178, 220, 301
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 29
Ama87I CYCGRG 3 cut(s) 125, 186, 221
AoxI GGCC 1 cut(s) 183
AspA2I CCTAGG 1 cut(s) 103
AspS9I GGNCC 5 cut(s) 100, 121, 130, 150, 184
AsuC2I CCSGG 4 cut(s) 148, 166, 187, 188
AsuHPI GGTGA 2 cut(s) 223, 460
AvaI CYCGRG 3 cut(s) 125, 186, 221
AvaII GGWCC 4 cut(s) 100, 121, 130, 150
AvrII CCTAGG 1 cut(s) 103
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 180
BciT130I CCWGG 3 cut(s) 173, 182, 209
BcnI CCSGG 4 cut(s) 148, 166, 187, 188
BcoDI GTCTC 1 cut(s) 29
BcuI ACTAGT 1 cut(s) 290
BfaI CTAG 2 cut(s) 104, 291
BlnI CCTAGG 1 cut(s) 103
BmcAI AGTACT 1 cut(s) 479
Bme1390I CCNGG 7 cut(s) 148, 166, 173, 182, 187, 188, 209
Bme18I GGWCC 4 cut(s) 100, 121, 130, 150
BmeT110I CYCGRG 3 cut(s) 125, 186, 221
BmgT120I GGNCC 5 cut(s) 100, 121, 130, 150, 184
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 7 cut(s) 148, 166, 173, 182, 187, 188, 209
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 4 cut(s) 148, 166, 187, 188
BsaJI CCNNGG 2 cut(s) 103, 186
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 187
BseBI CCWGG 3 cut(s) 173, 182, 209
BseDI CCNNGG 2 cut(s) 103, 186
BseLI CCNNNNNNNGG 2 cut(s) 172, 187
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 2 cut(s) 415, 425
BshFI GGCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 3 cut(s) 125, 186, 221
BsiSI CCGG 3 cut(s) 148, 166, 187
BslI CCNNNNNNNGG 2 cut(s) 172, 187
BsmAI GTCTC 1 cut(s) 29
BsmBI CGTCTC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 1 cut(s) 185
BsoBI CYCGRG 3 cut(s) 125, 186, 221
Bsp1286I GDGCHC 1 cut(s) 180
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 170
BspQI GCTCTTC 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 103, 186
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 3 cut(s) 173, 182, 209
Bst4CI ACNGT 2 cut(s) 248, 482
Bst6I CTCTTC 3 cut(s) 291, 311, 491
BstDEI CTNAG 1 cut(s) 53
BstMAI GTCTC 1 cut(s) 29
BstMWI GCNNNNNNNGC 3 cut(s) 54, 191, 200
BstNI CCWGG 3 cut(s) 173, 182, 209
BstSCI CCNGG 7 cut(s) 146, 164, 171, 180, 185, 186, 207
BstV2I GAAGAC 1 cut(s) 101
BsuRI GGCC 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 119
Cfr13I GGNCC 5 cut(s) 100, 121, 130, 150, 184
Cfr9I CCCGGG 1 cut(s) 186
CsiI ACCWGGT 1 cut(s) 207
Csp6I GTAC 2 cut(s) 244, 478
CviJI RGCY 9 cut(s) 14, 62, 72, 86, 178, 185, 194, 220, 301
CviKI_1 RGCY 9 cut(s) 14, 62, 72, 86, 178, 185, 194, 220, 301
CviQI GTAC 2 cut(s) 244, 478
DdeI CTNAG 1 cut(s) 53
DraI TTTAAA 1 cut(s) 270
Eam1104I CTCTTC 3 cut(s) 291, 311, 491
EarI CTCTTC 3 cut(s) 291, 311, 491
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 4 cut(s) 100, 121, 130, 150
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 95
Eco88I CYCGRG 3 cut(s) 125, 186, 221
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 171, 180, 207
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 29
FaiI YATR 3 cut(s) 11, 66, 98
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 2 cut(s) 104, 291
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 1 cut(s) 185
HapII CCGG 3 cut(s) 148, 166, 187
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 3 cut(s) 140, 224, 253
HpaII CCGG 3 cut(s) 148, 166, 187
HphI GGTGA 2 cut(s) 223, 460
Hpy188I TCNGA 3 cut(s) 19, 54, 328
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 7 cut(s) 112, 226, 346, 409, 430, 439, 499
HpyCH4III ACNGT 2 cut(s) 248, 482
HpyCH4V TGCA 2 cut(s) 262, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 191, 200
HpyF3I CTNAG 1 cut(s) 53
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 2 cut(s) 175, 183
MabI ACCWGGT 1 cut(s) 207
MaeI CTAG 2 cut(s) 104, 291
MaeIII GTNAC 1 cut(s) 254
MboII GAAGA 7 cut(s) 101, 296, 308, 328, 454, 508, 514
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 1 cut(s) 279
MlyI GAGTC 3 cut(s) 134, 233, 262
MmeI TCCRAC 1 cut(s) 450
MseI TTAA 2 cut(s) 269, 278
MspI CCGG 3 cut(s) 148, 166, 187
MspR9I CCNGG 7 cut(s) 148, 166, 173, 182, 187, 188, 209
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 3 cut(s) 173, 182, 209
MwoI GCNNNNNNNGC 3 cut(s) 54, 191, 200
NciI CCSGG 4 cut(s) 148, 166, 187, 188
NlaIV GGNNCC 1 cut(s) 170
NmuCI GTSAC 1 cut(s) 254
PaeR7I CTCGAG 2 cut(s) 125, 221
PciSI GCTCTTC 1 cut(s) 291
PctI GAATGC 1 cut(s) 366
PfoI TCCNGGA 1 cut(s) 171
PleI GAGTC 3 cut(s) 134, 232, 261
PpsI GAGTC 3 cut(s) 134, 232, 261
PpuMI RGGWCCY 1 cut(s) 121
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 3 cut(s) 171, 180, 207
PspGI CCWGG 3 cut(s) 171, 180, 207
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 5 cut(s) 100, 121, 130, 150, 184
PspPPI RGGWCCY 1 cut(s) 121
PspXI VCTCGAGB 2 cut(s) 125, 221
RsaI GTAC 2 cut(s) 245, 479
RsaNI GTAC 2 cut(s) 244, 478
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 269, 278
Sau96I GGNCC 5 cut(s) 100, 121, 130, 150, 184
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 3 cut(s) 134, 233, 262
ScrFI CCNGG 7 cut(s) 148, 166, 173, 182, 187, 188, 209
SduI GDGCHC 1 cut(s) 180
SexAI ACCWGGT 1 cut(s) 207
Sfr274I CTCGAG 2 cut(s) 125, 221
SinI GGWCC 4 cut(s) 100, 121, 130, 150
SlaI CTCGAG 2 cut(s) 125, 221
SmaI CCCGGG 1 cut(s) 188
SmlI CTYRAG 3 cut(s) 125, 221, 365
SmoI CTYRAG 3 cut(s) 125, 221, 365
SpeI ACTAGT 1 cut(s) 290
Sse9I AATT 1 cut(s) 279
SspMI CTAG 2 cut(s) 104, 291
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 7 cut(s) 146, 164, 171, 180, 185, 186, 207
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 248, 482
TaqI TCGA 4 cut(s) 126, 138, 143, 222
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 477
Tru1I TTAA 2 cut(s) 269, 278
Tru9I TTAA 2 cut(s) 269, 278
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 254
TspMI CCCGGG 1 cut(s) 186
TspRI CASTG 1 cut(s) 119
VpaK11BI GGWCC 4 cut(s) 100, 121, 130, 150
XhoI CTCGAG 2 cut(s) 125, 221
XmaI CCCGGG 1 cut(s) 186
XmaJI CCTAGG 1 cut(s) 103
XspI CTAG 2 cut(s) 104, 291
ZrmI AGTACT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.